A novel peptide with potent and broad-spectrum antiviral activities against multiple respiratory viruses
Identifieur interne : 000742 ( Pmc/Checkpoint ); précédent : 000741; suivant : 000743A novel peptide with potent and broad-spectrum antiviral activities against multiple respiratory viruses
Auteurs : Hanjun Zhao [République populaire de Chine] ; Jie Zhou [République populaire de Chine] ; Ke Zhang [République populaire de Chine] ; Hin Chu [République populaire de Chine] ; Dabin Liu [République populaire de Chine] ; Vincent Kwok-Man Poon [République populaire de Chine] ; Chris Chung-Sing Chan [République populaire de Chine] ; Ho-Chuen Leung [République populaire de Chine] ; Ng Fai [République populaire de Chine] ; Yong-Ping Lin [République populaire de Chine] ; Anna Jin-Xia Zhang [République populaire de Chine] ; Dong-Yan Jin [République populaire de Chine] ; Kwok-Yung Yuen [République populaire de Chine] ; Bo-Jian Zheng [République populaire de Chine]Source :
- Scientific Reports [ 2045-2322 ] ; 2016.
Abstract
A safe, potent and broad-spectrum antiviral is urgently needed to combat emerging respiratory viruses. In light of the broad antiviral activity of β-defensins, we tested the antiviral activity of 11 peptides derived from mouse β-defensin-4 and found that a short peptide, P9, exhibited potent and broad-spectrum antiviral effects against multiple respiratory viruses
The online version of this article (doi:10.1038/srep22008) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1038/srep22008
PubMed: 26911565
PubMed Central: 4766503
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
PMC:4766503Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A novel peptide with potent and broad-spectrum antiviral activities against multiple respiratory viruses</title><author><name sortKey="Zhao, Hanjun" sort="Zhao, Hanjun" uniqKey="Zhao H" first="Hanjun" last="Zhao">Hanjun Zhao</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.194645.b</institution-id><institution-id institution-id-type="ISNI">0000000121742757</institution-id><institution>Department of Microbiology,</institution><institution>The University of Hong Kong,</institution></institution-wrap>Hong Kong, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Zhou, Jie" sort="Zhou, Jie" uniqKey="Zhou J" first="Jie" last="Zhou">Jie Zhou</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.194645.b</institution-id><institution-id institution-id-type="ISNI">0000000121742757</institution-id><institution>Department of Microbiology,</institution><institution>The University of Hong Kong,</institution></institution-wrap>Hong Kong, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Zhang, Ke" sort="Zhang, Ke" uniqKey="Zhang K" first="Ke" last="Zhang">Ke Zhang</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.194645.b</institution-id><institution-id institution-id-type="ISNI">0000000121742757</institution-id><institution>Department of Microbiology,</institution><institution>The University of Hong Kong,</institution></institution-wrap>Hong Kong, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Chu, Hin" sort="Chu, Hin" uniqKey="Chu H" first="Hin" last="Chu">Hin Chu</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.194645.b</institution-id><institution-id institution-id-type="ISNI">0000000121742757</institution-id><institution>Department of Microbiology,</institution><institution>The University of Hong Kong,</institution></institution-wrap>Hong Kong, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Liu, Dabin" sort="Liu, Dabin" uniqKey="Liu D" first="Dabin" last="Liu">Dabin Liu</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.194645.b</institution-id><institution-id institution-id-type="ISNI">0000000121742757</institution-id><institution>Department of Microbiology,</institution><institution>The University of Hong Kong,</institution></institution-wrap>Hong Kong, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Poon, Vincent Kwok Man" sort="Poon, Vincent Kwok Man" uniqKey="Poon V" first="Vincent Kwok-Man" last="Poon">Vincent Kwok-Man Poon</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.194645.b</institution-id><institution-id institution-id-type="ISNI">0000000121742757</institution-id><institution>Department of Microbiology,</institution><institution>The University of Hong Kong,</institution></institution-wrap>Hong Kong, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Chan, Chris Chung Sing" sort="Chan, Chris Chung Sing" uniqKey="Chan C" first="Chris Chung-Sing" last="Chan">Chris Chung-Sing Chan</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.194645.b</institution-id><institution-id institution-id-type="ISNI">0000000121742757</institution-id><institution>Department of Microbiology,</institution><institution>The University of Hong Kong,</institution></institution-wrap>Hong Kong, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Leung, Ho Chuen" sort="Leung, Ho Chuen" uniqKey="Leung H" first="Ho-Chuen" last="Leung">Ho-Chuen Leung</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.194645.b</institution-id><institution-id institution-id-type="ISNI">0000000121742757</institution-id><institution>Department of Microbiology,</institution><institution>The University of Hong Kong,</institution></institution-wrap>Hong Kong, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Fai, Ng" sort="Fai, Ng" uniqKey="Fai N" first="Ng" last="Fai">Ng Fai</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.194645.b</institution-id><institution-id institution-id-type="ISNI">0000000121742757</institution-id><institution>Department of Microbiology,</institution><institution>The University of Hong Kong,</institution></institution-wrap>Hong Kong, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Lin, Yong Ping" sort="Lin, Yong Ping" uniqKey="Lin Y" first="Yong-Ping" last="Lin">Yong-Ping Lin</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.194645.b</institution-id><institution-id institution-id-type="ISNI">0000000121742757</institution-id><institution>Department of Microbiology,</institution><institution>The University of Hong Kong,</institution></institution-wrap>Hong Kong, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Zhang, Anna Jin Xia" sort="Zhang, Anna Jin Xia" uniqKey="Zhang A" first="Anna Jin-Xia" last="Zhang">Anna Jin-Xia Zhang</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.194645.b</institution-id><institution-id institution-id-type="ISNI">0000000121742757</institution-id><institution>Department of Microbiology,</institution><institution>The University of Hong Kong,</institution></institution-wrap>Hong Kong, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Jin, Dong Yan" sort="Jin, Dong Yan" uniqKey="Jin D" first="Dong-Yan" last="Jin">Dong-Yan Jin</name><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.194645.b</institution-id><institution-id institution-id-type="ISNI">0000000121742757</institution-id><institution>School of Biomedical Sciences, The University of Hong Kong,</institution></institution-wrap>Hong Kong, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.194645.b</institution-id><institution-id institution-id-type="ISNI">0000000121742757</institution-id><institution>Department of Microbiology,</institution><institution>The University of Hong Kong,</institution></institution-wrap>Hong Kong, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Zheng, Bo Jian" sort="Zheng, Bo Jian" uniqKey="Zheng B" first="Bo-Jian" last="Zheng">Bo-Jian Zheng</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.194645.b</institution-id><institution-id institution-id-type="ISNI">0000000121742757</institution-id><institution>Department of Microbiology,</institution><institution>The University of Hong Kong,</institution></institution-wrap>Hong Kong, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hong Kong</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">26911565</idno><idno type="pmc">4766503</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4766503</idno><idno type="RBID">PMC:4766503</idno><idno type="doi">10.1038/srep22008</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">000472</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000472</idno><idno type="wicri:Area/Pmc/Curation">000472</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000472</idno><idno type="wicri:Area/Pmc/Checkpoint">000742</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000742</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">A novel peptide with potent and broad-spectrum antiviral activities against multiple respiratory viruses</title><author><name sortKey="Zhao, Hanjun" sort="Zhao, Hanjun" uniqKey="Zhao H" first="Hanjun" last="Zhao">Hanjun Zhao</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.194645.b</institution-id><institution-id institution-id-type="ISNI">0000000121742757</institution-id><institution>Department of Microbiology,</institution><institution>The University of Hong Kong,</institution></institution-wrap>Hong Kong, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Zhou, Jie" sort="Zhou, Jie" uniqKey="Zhou J" first="Jie" last="Zhou">Jie Zhou</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.194645.b</institution-id><institution-id institution-id-type="ISNI">0000000121742757</institution-id><institution>Department of Microbiology,</institution><institution>The University of Hong Kong,</institution></institution-wrap>Hong Kong, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Zhang, Ke" sort="Zhang, Ke" uniqKey="Zhang K" first="Ke" last="Zhang">Ke Zhang</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.194645.b</institution-id><institution-id institution-id-type="ISNI">0000000121742757</institution-id><institution>Department of Microbiology,</institution><institution>The University of Hong Kong,</institution></institution-wrap>Hong Kong, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Chu, Hin" sort="Chu, Hin" uniqKey="Chu H" first="Hin" last="Chu">Hin Chu</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.194645.b</institution-id><institution-id institution-id-type="ISNI">0000000121742757</institution-id><institution>Department of Microbiology,</institution><institution>The University of Hong Kong,</institution></institution-wrap>Hong Kong, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Liu, Dabin" sort="Liu, Dabin" uniqKey="Liu D" first="Dabin" last="Liu">Dabin Liu</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.194645.b</institution-id><institution-id institution-id-type="ISNI">0000000121742757</institution-id><institution>Department of Microbiology,</institution><institution>The University of Hong Kong,</institution></institution-wrap>Hong Kong, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Poon, Vincent Kwok Man" sort="Poon, Vincent Kwok Man" uniqKey="Poon V" first="Vincent Kwok-Man" last="Poon">Vincent Kwok-Man Poon</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.194645.b</institution-id><institution-id institution-id-type="ISNI">0000000121742757</institution-id><institution>Department of Microbiology,</institution><institution>The University of Hong Kong,</institution></institution-wrap>Hong Kong, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Chan, Chris Chung Sing" sort="Chan, Chris Chung Sing" uniqKey="Chan C" first="Chris Chung-Sing" last="Chan">Chris Chung-Sing Chan</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.194645.b</institution-id><institution-id institution-id-type="ISNI">0000000121742757</institution-id><institution>Department of Microbiology,</institution><institution>The University of Hong Kong,</institution></institution-wrap>Hong Kong, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Leung, Ho Chuen" sort="Leung, Ho Chuen" uniqKey="Leung H" first="Ho-Chuen" last="Leung">Ho-Chuen Leung</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.194645.b</institution-id><institution-id institution-id-type="ISNI">0000000121742757</institution-id><institution>Department of Microbiology,</institution><institution>The University of Hong Kong,</institution></institution-wrap>Hong Kong, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Fai, Ng" sort="Fai, Ng" uniqKey="Fai N" first="Ng" last="Fai">Ng Fai</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.194645.b</institution-id><institution-id institution-id-type="ISNI">0000000121742757</institution-id><institution>Department of Microbiology,</institution><institution>The University of Hong Kong,</institution></institution-wrap>Hong Kong, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Lin, Yong Ping" sort="Lin, Yong Ping" uniqKey="Lin Y" first="Yong-Ping" last="Lin">Yong-Ping Lin</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.194645.b</institution-id><institution-id institution-id-type="ISNI">0000000121742757</institution-id><institution>Department of Microbiology,</institution><institution>The University of Hong Kong,</institution></institution-wrap>Hong Kong, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Zhang, Anna Jin Xia" sort="Zhang, Anna Jin Xia" uniqKey="Zhang A" first="Anna Jin-Xia" last="Zhang">Anna Jin-Xia Zhang</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.194645.b</institution-id><institution-id institution-id-type="ISNI">0000000121742757</institution-id><institution>Department of Microbiology,</institution><institution>The University of Hong Kong,</institution></institution-wrap>Hong Kong, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Jin, Dong Yan" sort="Jin, Dong Yan" uniqKey="Jin D" first="Dong-Yan" last="Jin">Dong-Yan Jin</name><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.194645.b</institution-id><institution-id institution-id-type="ISNI">0000000121742757</institution-id><institution>School of Biomedical Sciences, The University of Hong Kong,</institution></institution-wrap>Hong Kong, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.194645.b</institution-id><institution-id institution-id-type="ISNI">0000000121742757</institution-id><institution>Department of Microbiology,</institution><institution>The University of Hong Kong,</institution></institution-wrap>Hong Kong, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hong Kong</wicri:regionArea></affiliation></author><author><name sortKey="Zheng, Bo Jian" sort="Zheng, Bo Jian" uniqKey="Zheng B" first="Bo-Jian" last="Zheng">Bo-Jian Zheng</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.194645.b</institution-id><institution-id institution-id-type="ISNI">0000000121742757</institution-id><institution>Department of Microbiology,</institution><institution>The University of Hong Kong,</institution></institution-wrap>Hong Kong, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hong Kong</wicri:regionArea></affiliation></author></analytic><series><title level="j">Scientific Reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>A safe, potent and broad-spectrum antiviral is urgently needed to combat emerging respiratory viruses. In light of the broad antiviral activity of β-defensins, we tested the antiviral activity of 11 peptides derived from mouse β-defensin-4 and found that a short peptide, P9, exhibited potent and broad-spectrum antiviral effects against multiple respiratory viruses <italic>in vitro</italic> and <italic>in vivo</italic>, including influenza A virus H1N1, H3N2, H5N1, H7N7, H7N9, SARS-CoV and MERS-CoV. The antiviral activity of P9 was attributed to its high-affinity binding to viral glycoproteins, as well as the abundance of basic amino acids in its composition. After binding viral particles through viral surface glycoproteins, P9 entered into cells together with the viruses via endocytosis and prevented endosomal acidification, which blocked membrane fusion and subsequent viral RNA release. This study has paved the avenue for developing new prophylactic and therapeutic agents with broad-spectrum antiviral activities.</p><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1038/srep22008) contains supplementary material, which is available to authorized users.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Horimoto, T" uniqKey="Horimoto T">T Horimoto</name></author><author><name sortKey="Kawaoka, Y" uniqKey="Kawaoka Y">Y Kawaoka</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Watanabe, T" uniqKey="Watanabe T">T Watanabe</name></author><author><name sortKey="Watanabe, S" uniqKey="Watanabe S">S Watanabe</name></author><author><name sortKey="Maher, Ea" uniqKey="Maher E">EA Maher</name></author><author><name sortKey="Neumann, G" uniqKey="Neumann G">G Neumann</name></author><author><name sortKey="Kawaoka, Y" uniqKey="Kawaoka Y">Y Kawaoka</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Du, L" uniqKey="Du L">L Du</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Castle, Sc" uniqKey="Castle S">SC Castle</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Luscher Mattli, M" uniqKey="Luscher Mattli M">M Luscher-Mattli</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zimmermann, Gr" uniqKey="Zimmermann G">GR Zimmermann</name></author><author><name sortKey="Legault, P" uniqKey="Legault P">P Legault</name></author><author><name sortKey="Selsted, Me" uniqKey="Selsted M">ME Selsted</name></author><author><name sortKey="Pardi, A" uniqKey="Pardi A">A Pardi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Valore, Ev" uniqKey="Valore E">EV Valore</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bals, R" uniqKey="Bals R">R Bals</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Quinones Mateu, Me" uniqKey="Quinones Mateu M">ME Quinones-Mateu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Leikina, E" uniqKey="Leikina E">E Leikina</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ryan, Lk" uniqKey="Ryan L">LK Ryan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Klotman, Me" uniqKey="Klotman M">ME Klotman</name></author><author><name sortKey="Chang, Tl" uniqKey="Chang T">TL Chang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ding, J" uniqKey="Ding J">J Ding</name></author><author><name sortKey="Chou, Yy" uniqKey="Chou Y">YY Chou</name></author><author><name sortKey="Chang, Tl" uniqKey="Chang T">TL Chang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Feng, Z" uniqKey="Feng Z">Z Feng</name></author><author><name sortKey="Dubyak, Gr" uniqKey="Dubyak G">GR Dubyak</name></author><author><name sortKey="Lederman, Mm" uniqKey="Lederman M">MM Lederman</name></author><author><name sortKey="Weinberg, A" uniqKey="Weinberg A">A Weinberg</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sun, L" uniqKey="Sun L">L Sun</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kota, S" uniqKey="Kota S">S Kota</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zasloff, M" uniqKey="Zasloff M">M Zasloff</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bai, Y" uniqKey="Bai Y">Y Bai</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Matlin, Ks" uniqKey="Matlin K">KS Matlin</name></author><author><name sortKey="Reggio, H" uniqKey="Reggio H">H Reggio</name></author><author><name sortKey="Helenius, A" uniqKey="Helenius A">A Helenius</name></author><author><name sortKey="Simons, K" uniqKey="Simons K">K Simons</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lakadamyali, M" uniqKey="Lakadamyali M">M Lakadamyali</name></author><author><name sortKey="Rust, Mj" uniqKey="Rust M">MJ Rust</name></author><author><name sortKey="Babcock, Hp" uniqKey="Babcock H">HP Babcock</name></author><author><name sortKey="Zhuang, X" uniqKey="Zhuang X">X Zhuang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kuznik, A" uniqKey="Kuznik A">A Kuznik</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Schmid, S" uniqKey="Schmid S">S Schmid</name></author><author><name sortKey="Fuchs, R" uniqKey="Fuchs R">R Fuchs</name></author><author><name sortKey="Kielian, M" uniqKey="Kielian M">M Kielian</name></author><author><name sortKey="Helenius, A" uniqKey="Helenius A">A Helenius</name></author><author><name sortKey="Mellman, I" uniqKey="Mellman I">I Mellman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Perez, L" uniqKey="Perez L">L Perez</name></author><author><name sortKey="Carrasco, L" uniqKey="Carrasco L">L Carrasco</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, Ks" uniqKey="Li K">KS Li</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gao, R" uniqKey="Gao R">R Gao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gubareva, L" uniqKey="Gubareva L">L Gubareva</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Smith, Ae" uniqKey="Smith A">AE Smith</name></author><author><name sortKey="Helenius, A" uniqKey="Helenius A">A Helenius</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Das, K" uniqKey="Das K">K Das</name></author><author><name sortKey="Aramini, Jm" uniqKey="Aramini J">JM Aramini</name></author><author><name sortKey="Ma, Lc" uniqKey="Ma L">LC Ma</name></author><author><name sortKey="Krug, Rm" uniqKey="Krug R">RM Krug</name></author><author><name sortKey="Arnold, E" uniqKey="Arnold E">E Arnold</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zheng, B" uniqKey="Zheng B">B Zheng</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zheng, Bj" uniqKey="Zheng B">BJ Zheng</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhong, Ns" uniqKey="Zhong N">NS Zhong</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Puigbo, P" uniqKey="Puigbo P">P Puigbo</name></author><author><name sortKey="Guzman, E" uniqKey="Guzman E">E Guzman</name></author><author><name sortKey="Romeu, A" uniqKey="Romeu A">A Romeu</name></author><author><name sortKey="Garcia Vallve, S" uniqKey="Garcia Vallve S">S Garcia-Vallve</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Gong, T" uniqKey="Gong T">T Gong</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sui, Hy" uniqKey="Sui H">HY Sui</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pauwels, R" uniqKey="Pauwels R">R Pauwels</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Guan, Y" uniqKey="Guan Y">Y Guan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Du, L" uniqKey="Du L">L Du</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Sci Rep</journal-id><journal-id journal-id-type="iso-abbrev">Sci Rep</journal-id><journal-title-group><journal-title>Scientific Reports</journal-title></journal-title-group><issn pub-type="epub">2045-2322</issn><publisher><publisher-name>Nature Publishing Group UK</publisher-name><publisher-loc>London</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">26911565</article-id><article-id pub-id-type="pmc">4766503</article-id><article-id pub-id-type="publisher-id">BFsrep22008</article-id><article-id pub-id-type="doi">10.1038/srep22008</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>A novel peptide with potent and broad-spectrum antiviral activities against multiple respiratory viruses</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Zhao</surname><given-names>Hanjun</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Zhou</surname><given-names>Jie</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Zhang</surname><given-names>Ke</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Chu</surname><given-names>Hin</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Liu</surname><given-names>Dabin</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Poon</surname><given-names>Vincent Kwok-Man</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Chan</surname><given-names>Chris Chung-Sing</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Leung</surname><given-names>Ho-Chuen</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Fai</surname><given-names>Ng</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Lin</surname><given-names>Yong-Ping</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Zhang</surname><given-names>Anna Jin-Xia</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Jin</surname><given-names>Dong-Yan</given-names></name><xref ref-type="aff" rid="Aff2">2</xref></contrib><contrib contrib-type="author"><name><surname>Yuen</surname><given-names>Kwok-Yung</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Zheng</surname><given-names>Bo-Jian</given-names></name><address><email>bzheng@hkucc.hku.hk</email></address><xref ref-type="aff" rid="Aff1">1</xref></contrib><aff id="Aff1"><label>1</label><institution-wrap><institution-id institution-id-type="GRID">grid.194645.b</institution-id><institution-id institution-id-type="ISNI">0000000121742757</institution-id><institution>Department of Microbiology,</institution><institution>The University of Hong Kong,</institution></institution-wrap>Hong Kong, China</aff><aff id="Aff2"><label>2</label><institution-wrap><institution-id institution-id-type="GRID">grid.194645.b</institution-id><institution-id institution-id-type="ISNI">0000000121742757</institution-id><institution>School of Biomedical Sciences, The University of Hong Kong,</institution></institution-wrap>Hong Kong, China</aff></contrib-group><pub-date pub-type="epub"><day>25</day><month>2</month><year>2016</year></pub-date><pub-date pub-type="pmc-release"><day>25</day><month>2</month><year>2016</year></pub-date><pub-date pub-type="collection"><year>2016</year></pub-date><volume>6</volume><elocation-id>22008</elocation-id><history><date date-type="received"><day>4</day><month>11</month><year>2015</year></date><date date-type="accepted"><day>3</day><month>2</month><year>2016</year></date></history><permissions><copyright-statement>© The Author(s) 2016</copyright-statement><license license-type="OpenAccess"><license-p>This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">http://creativecommons.org/licenses/by/4.0/</ext-link></license-p></license></permissions><abstract id="Abs1"><p>A safe, potent and broad-spectrum antiviral is urgently needed to combat emerging respiratory viruses. In light of the broad antiviral activity of β-defensins, we tested the antiviral activity of 11 peptides derived from mouse β-defensin-4 and found that a short peptide, P9, exhibited potent and broad-spectrum antiviral effects against multiple respiratory viruses <italic>in vitro</italic> and <italic>in vivo</italic>, including influenza A virus H1N1, H3N2, H5N1, H7N7, H7N9, SARS-CoV and MERS-CoV. The antiviral activity of P9 was attributed to its high-affinity binding to viral glycoproteins, as well as the abundance of basic amino acids in its composition. After binding viral particles through viral surface glycoproteins, P9 entered into cells together with the viruses via endocytosis and prevented endosomal acidification, which blocked membrane fusion and subsequent viral RNA release. This study has paved the avenue for developing new prophylactic and therapeutic agents with broad-spectrum antiviral activities.</p><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1038/srep22008) contains supplementary material, which is available to authorized users.</p></sec></abstract><kwd-group kwd-group-type="npg-subject"><title>Subject terms</title><kwd>Drug discovery</kwd><kwd>Antiviral agents</kwd></kwd-group><custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name><meta-value>© The Author(s) 2016</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Zhao, Hanjun" sort="Zhao, Hanjun" uniqKey="Zhao H" first="Hanjun" last="Zhao">Hanjun Zhao</name></noRegion><name sortKey="Chan, Chris Chung Sing" sort="Chan, Chris Chung Sing" uniqKey="Chan C" first="Chris Chung-Sing" last="Chan">Chris Chung-Sing Chan</name><name sortKey="Chu, Hin" sort="Chu, Hin" uniqKey="Chu H" first="Hin" last="Chu">Hin Chu</name><name sortKey="Fai, Ng" sort="Fai, Ng" uniqKey="Fai N" first="Ng" last="Fai">Ng Fai</name><name sortKey="Jin, Dong Yan" sort="Jin, Dong Yan" uniqKey="Jin D" first="Dong-Yan" last="Jin">Dong-Yan Jin</name><name sortKey="Leung, Ho Chuen" sort="Leung, Ho Chuen" uniqKey="Leung H" first="Ho-Chuen" last="Leung">Ho-Chuen Leung</name><name sortKey="Lin, Yong Ping" sort="Lin, Yong Ping" uniqKey="Lin Y" first="Yong-Ping" last="Lin">Yong-Ping Lin</name><name sortKey="Liu, Dabin" sort="Liu, Dabin" uniqKey="Liu D" first="Dabin" last="Liu">Dabin Liu</name><name sortKey="Poon, Vincent Kwok Man" sort="Poon, Vincent Kwok Man" uniqKey="Poon V" first="Vincent Kwok-Man" last="Poon">Vincent Kwok-Man Poon</name><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name><name sortKey="Zhang, Anna Jin Xia" sort="Zhang, Anna Jin Xia" uniqKey="Zhang A" first="Anna Jin-Xia" last="Zhang">Anna Jin-Xia Zhang</name><name sortKey="Zhang, Ke" sort="Zhang, Ke" uniqKey="Zhang K" first="Ke" last="Zhang">Ke Zhang</name><name sortKey="Zheng, Bo Jian" sort="Zheng, Bo Jian" uniqKey="Zheng B" first="Bo-Jian" last="Zheng">Bo-Jian Zheng</name><name sortKey="Zhou, Jie" sort="Zhou, Jie" uniqKey="Zhou J" first="Jie" last="Zhou">Jie Zhou</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Pmc/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000742 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd -nk 000742 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Pmc
|étape= Checkpoint
|type= RBID
|clé= PMC:4766503
|texte= A novel peptide with potent and broad-spectrum antiviral activities against multiple respiratory viruses
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/RBID.i -Sk "pubmed:26911565" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |