Cryo-EM structures of MERS-CoV and SARS-CoV spike glycoproteins reveal the dynamic receptor binding domains
Identifieur interne : 000637 ( Pmc/Checkpoint ); précédent : 000636; suivant : 000638Cryo-EM structures of MERS-CoV and SARS-CoV spike glycoproteins reveal the dynamic receptor binding domains
Auteurs : Yuan Yuan [République populaire de Chine] ; Duanfang Cao [République populaire de Chine] ; Yanfang Zhang [République populaire de Chine] ; Jun Ma [République populaire de Chine] ; Jianxun Qi [République populaire de Chine] ; Qihui Wang [République populaire de Chine] ; Guangwen Lu ; Ying Wu [République populaire de Chine] ; Jinghua Yan [République populaire de Chine] ; Yi Shi [République populaire de Chine] ; Xinzheng Zhang [République populaire de Chine] ; George F. Gao [République populaire de Chine]Source :
- Nature Communications [ 2041-1723 ] ; 2017.
Abstract
The envelope spike (S) proteins of MERS-CoV and SARS-CoV determine the virus host tropism and entry into host cells, and constitute a promising target for the development of prophylactics and therapeutics. Here, we present high-resolution structures of the trimeric MERS-CoV and SARS-CoV S proteins in its pre-fusion conformation by single particle cryo-electron microscopy. The overall structures resemble that from other coronaviruses including HKU1, MHV and NL63 reported recently, with the exception of the receptor binding domain (RBD). We captured two states of the RBD with receptor binding region either buried (lying state) or exposed (standing state), demonstrating an inherently flexible RBD readily recognized by the receptor. Further sequence conservation analysis of six human-infecting coronaviruses revealed that the fusion peptide, HR1 region and the central helix are potential targets for eliciting broadly neutralizing antibodies.
The online version of this article (doi:10.1038/ncomms15092) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1038/ncomms15092
PubMed: 28393837
PubMed Central: 5394239
Affiliations:
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institution-id-type="ISNI">0000 0001 2331 6153</institution-id><institution>School of Basic Medical Sciences, Wuhan University,</institution></institution-wrap>Wuhan, 430071 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Wuhan</wicri:regionArea></affiliation></author><author><name sortKey="Yan, Jinghua" sort="Yan, Jinghua" uniqKey="Yan J" first="Jinghua" last="Yan">Jinghua Yan</name><affiliation wicri:level="1"><nlm:aff id="Aff5"><institution-wrap><institution-id institution-id-type="GRID">grid.458488.d</institution-id><institution-id institution-id-type="ISNI">0000 0004 0627 1442</institution-id><institution>CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences,</institution></institution-wrap>Beijing, 100101 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing</wicri:regionArea></affiliation><affiliation 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institution-id-type="GRID">grid.410726.6</institution-id><institution-id institution-id-type="ISNI">0000 0004 1797 8419</institution-id><institution>Medical School, University of Chinese Academy of Sciences,</institution></institution-wrap>Beijing, 101408 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Shi, Yi" sort="Shi, Yi" uniqKey="Shi Y" first="Yi" last="Shi">Yi Shi</name><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.458488.d</institution-id><institution-id institution-id-type="ISNI">0000 0004 0627 1442</institution-id><institution>CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences,</institution></institution-wrap>Beijing, 100101 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff8"><institution-wrap><institution-id institution-id-type="GRID">grid.410741.7</institution-id><institution>Shenzhen Key Laboratory of Pathogen and Immunity, Shenzhen Third People's Hospital,</institution></institution-wrap>Shenzhen, 518112 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Shenzhen</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff9"><institution-wrap><institution-id institution-id-type="GRID">grid.9227.e</institution-id><institution-id institution-id-type="ISNI">0000000119573309</institution-id><institution>Center for Influenza Research and Early-warning, Chinese Academy of Sciences (CASCIRE),</institution></institution-wrap>Beijing, 100101 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff10"><institution-wrap><institution-id institution-id-type="GRID">grid.410726.6</institution-id><institution-id institution-id-type="ISNI">0000 0004 1797 8419</institution-id><institution>Medical School, University of Chinese Academy of Sciences,</institution></institution-wrap>Beijing, 101408 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Zhang, Xinzheng" sort="Zhang, Xinzheng" uniqKey="Zhang X" first="Xinzheng" last="Zhang">Xinzheng Zhang</name><affiliation wicri:level="1"><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="GRID">grid.418856.6</institution-id><institution-id institution-id-type="ISNI">0000 0004 1792 5640</institution-id><institution>National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences,</institution></institution-wrap>Beijing, 100101 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff11"><institution-wrap><institution-id institution-id-type="GRID">grid.418856.6</institution-id><institution-id institution-id-type="ISNI">0000 0004 1792 5640</institution-id><institution>Center for Biological Imaging, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences,</institution></institution-wrap>Beijing, 100101 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Gao, George F" sort="Gao, George F" uniqKey="Gao G" first="George F." last="Gao">George F. Gao</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.59053.3a</institution-id><institution-id institution-id-type="ISNI">0000000121679639</institution-id><institution>School of Life Sciences, University of Science and Technology of China,</institution></institution-wrap>Hefei, 230026 Anhui China</nlm:aff><wicri:noCountry code="subfield">230026 Anhui China</wicri:noCountry></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.458488.d</institution-id><institution-id institution-id-type="ISNI">0000 0004 0627 1442</institution-id><institution>CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences,</institution></institution-wrap>Beijing, 100101 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing</wicri:regionArea></affiliation><affiliation wicri:level="3"><nlm:aff id="Aff4">Laboratory of Protein Engineering and Vaccines, Tianjin Institute of Biotechnology, Tianjin, 300308 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Laboratory of Protein Engineering and Vaccines, Tianjin Institute of Biotechnology, Tianjin</wicri:regionArea><placeName><settlement type="city">Tianjin</settlement></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff8"><institution-wrap><institution-id institution-id-type="GRID">grid.410741.7</institution-id><institution>Shenzhen Key Laboratory of Pathogen and Immunity, Shenzhen Third People's Hospital,</institution></institution-wrap>Shenzhen, 518112 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Shenzhen</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff9"><institution-wrap><institution-id institution-id-type="GRID">grid.9227.e</institution-id><institution-id institution-id-type="ISNI">0000000119573309</institution-id><institution>Center for Influenza Research and Early-warning, Chinese Academy of Sciences (CASCIRE),</institution></institution-wrap>Beijing, 100101 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff10"><institution-wrap><institution-id institution-id-type="GRID">grid.410726.6</institution-id><institution-id institution-id-type="ISNI">0000 0004 1797 8419</institution-id><institution>Medical School, University of Chinese Academy of Sciences,</institution></institution-wrap>Beijing, 101408 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff12"><institution-wrap><institution-id institution-id-type="GRID">grid.198530.6</institution-id><institution-id institution-id-type="ISNI">0000 0000 8803 2373</institution-id><institution>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (China CDC),</institution></institution-wrap>Beijing, 102206 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing</wicri:regionArea></affiliation></author></analytic><series><title level="j">Nature Communications</title><idno type="eISSN">2041-1723</idno><imprint><date when="2017">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="Par1">The envelope spike (S) proteins of MERS-CoV and SARS-CoV determine the virus host tropism and entry into host cells, and constitute a promising target for the development of prophylactics and therapeutics. Here, we present high-resolution structures of the trimeric MERS-CoV and SARS-CoV S proteins in its pre-fusion conformation by single particle cryo-electron microscopy. The overall structures resemble that from other coronaviruses including HKU1, MHV and NL63 reported recently, with the exception of the receptor binding domain (RBD). We captured two states of the RBD with receptor binding region either buried (lying state) or exposed (standing state), demonstrating an inherently flexible RBD readily recognized by the receptor. Further sequence conservation analysis of six human-infecting coronaviruses revealed that the fusion peptide, HR1 region and the central helix are potential targets for eliciting broadly neutralizing antibodies.</p><sec><title>Supplementary information</title><p>The online version of this article (doi:10.1038/ncomms15092) contains supplementary material, which is available to authorized users.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Weinstein, Ra" uniqKey="Weinstein R">RA Weinstein</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hijawi, B" uniqKey="Hijawi B">B Hijawi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zaki, Am" uniqKey="Zaki A">AM Zaki</name></author><author><name sortKey="Van Boheemen, S" uniqKey="Van Boheemen S">S van Boheemen</name></author><author><name sortKey="Bestebroer, Tm" uniqKey="Bestebroer T">TM 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Nat Commun</journal-id><journal-id journal-id-type="iso-abbrev">Nat Commun</journal-id><journal-title-group><journal-title>Nature Communications</journal-title></journal-title-group><issn pub-type="epub">2041-1723</issn><publisher><publisher-name>Nature Publishing Group UK</publisher-name><publisher-loc>London</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">28393837</article-id><article-id pub-id-type="pmc">5394239</article-id><article-id pub-id-type="publisher-id">BFncomms15092</article-id><article-id pub-id-type="doi">10.1038/ncomms15092</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Cryo-EM structures of MERS-CoV and SARS-CoV spike glycoproteins reveal the dynamic receptor binding domains</article-title></title-group><contrib-group><contrib contrib-type="author" equal-contrib="yes"><name><surname>Yuan</surname><given-names>Yuan</given-names></name><xref ref-type="aff" rid="Aff1">1</xref><xref ref-type="aff" rid="Aff2">2</xref></contrib><contrib contrib-type="author" equal-contrib="yes"><name><surname>Cao</surname><given-names>Duanfang</given-names></name><xref ref-type="aff" rid="Aff3">3</xref></contrib><contrib contrib-type="author" equal-contrib="yes"><name><surname>Zhang</surname><given-names>Yanfang</given-names></name><xref ref-type="aff" rid="Aff2">2</xref><xref ref-type="aff" rid="Aff4">4</xref></contrib><contrib contrib-type="author" equal-contrib="yes"><name><surname>Ma</surname><given-names>Jun</given-names></name><xref ref-type="aff" rid="Aff3">3</xref></contrib><contrib contrib-type="author"><name><surname>Qi</surname><given-names>Jianxun</given-names></name><xref ref-type="aff" rid="Aff2">2</xref></contrib><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Qihui</given-names></name><xref ref-type="aff" rid="Aff5">5</xref></contrib><contrib contrib-type="author"><name><surname>Lu</surname><given-names>Guangwen</given-names></name><xref ref-type="aff" rid="Aff6">6</xref></contrib><contrib contrib-type="author"><name><surname>Wu</surname><given-names>Ying</given-names></name><xref ref-type="aff" rid="Aff7">7</xref></contrib><contrib contrib-type="author"><name><surname>Yan</surname><given-names>Jinghua</given-names></name><xref ref-type="aff" rid="Aff5">5</xref><xref ref-type="aff" rid="Aff8">8</xref><xref ref-type="aff" rid="Aff9">9</xref><xref ref-type="aff" rid="Aff10">10</xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Shi</surname><given-names>Yi</given-names></name><address><email>shiyi@im.ac.cn</email></address><xref ref-type="aff" rid="Aff2">2</xref><xref ref-type="aff" rid="Aff8">8</xref><xref ref-type="aff" rid="Aff9">9</xref><xref ref-type="aff" rid="Aff10">10</xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Zhang</surname><given-names>Xinzheng</given-names></name><address><email>shiyi@im.ac.cn</email></address><xref ref-type="aff" rid="Aff3">3</xref><xref ref-type="aff" rid="Aff11">11</xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Gao</surname><given-names>George F.</given-names></name><address><email>shiyi@im.ac.cn</email></address><xref ref-type="aff" rid="Aff1">1</xref><xref ref-type="aff" rid="Aff2">2</xref><xref ref-type="aff" rid="Aff4">4</xref><xref ref-type="aff" rid="Aff8">8</xref><xref ref-type="aff" rid="Aff9">9</xref><xref ref-type="aff" rid="Aff10">10</xref><xref ref-type="aff" rid="Aff12">12</xref></contrib><aff id="Aff1"><label>1</label><institution-wrap><institution-id institution-id-type="GRID">grid.59053.3a</institution-id><institution-id institution-id-type="ISNI">0000000121679639</institution-id><institution>School of Life Sciences, University of Science and Technology of China,</institution></institution-wrap>Hefei, 230026 Anhui China</aff><aff id="Aff2"><label>2</label><institution-wrap><institution-id institution-id-type="GRID">grid.458488.d</institution-id><institution-id institution-id-type="ISNI">0000 0004 0627 1442</institution-id><institution>CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences,</institution></institution-wrap>Beijing, 100101 China</aff><aff id="Aff3"><label>3</label><institution-wrap><institution-id institution-id-type="GRID">grid.418856.6</institution-id><institution-id institution-id-type="ISNI">0000 0004 1792 5640</institution-id><institution>National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences,</institution></institution-wrap>Beijing, 100101 China</aff><aff id="Aff4"><label>4</label>Laboratory of Protein Engineering and Vaccines, Tianjin Institute of Biotechnology, Tianjin, 300308 China</aff><aff id="Aff5"><label>5</label><institution-wrap><institution-id institution-id-type="GRID">grid.458488.d</institution-id><institution-id institution-id-type="ISNI">0000 0004 0627 1442</institution-id><institution>CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences,</institution></institution-wrap>Beijing, 100101 China</aff><aff id="Aff6"><label>6</label>West China Hospital Emergency Department (WCHED), State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, and Collaborative Innovation Center of Biotherapy, Chengdu, 610041 Sichuan China</aff><aff id="Aff7"><label>7</label><institution-wrap><institution-id institution-id-type="GRID">grid.49470.3e</institution-id><institution-id institution-id-type="ISNI">0000 0001 2331 6153</institution-id><institution>School of Basic Medical Sciences, Wuhan University,</institution></institution-wrap>Wuhan, 430071 China</aff><aff id="Aff8"><label>8</label><institution-wrap><institution-id institution-id-type="GRID">grid.410741.7</institution-id><institution>Shenzhen Key Laboratory of Pathogen and Immunity, Shenzhen Third People's Hospital,</institution></institution-wrap>Shenzhen, 518112 China</aff><aff id="Aff9"><label>9</label><institution-wrap><institution-id institution-id-type="GRID">grid.9227.e</institution-id><institution-id institution-id-type="ISNI">0000000119573309</institution-id><institution>Center for Influenza Research and Early-warning, Chinese Academy of Sciences (CASCIRE),</institution></institution-wrap>Beijing, 100101 China</aff><aff id="Aff10"><label>10</label><institution-wrap><institution-id institution-id-type="GRID">grid.410726.6</institution-id><institution-id institution-id-type="ISNI">0000 0004 1797 8419</institution-id><institution>Medical School, University of Chinese Academy of Sciences,</institution></institution-wrap>Beijing, 101408 China</aff><aff id="Aff11"><label>11</label><institution-wrap><institution-id institution-id-type="GRID">grid.418856.6</institution-id><institution-id institution-id-type="ISNI">0000 0004 1792 5640</institution-id><institution>Center for Biological Imaging, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences,</institution></institution-wrap>Beijing, 100101 China</aff><aff id="Aff12"><label>12</label><institution-wrap><institution-id institution-id-type="GRID">grid.198530.6</institution-id><institution-id institution-id-type="ISNI">0000 0000 8803 2373</institution-id><institution>National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (China CDC),</institution></institution-wrap>Beijing, 102206 China</aff></contrib-group><pub-date pub-type="epub"><day>10</day><month>4</month><year>2017</year></pub-date><pub-date pub-type="pmc-release"><day>10</day><month>4</month><year>2017</year></pub-date><pub-date pub-type="collection"><year>2017</year></pub-date><volume>8</volume><elocation-id>15092</elocation-id><history><date date-type="received"><day>6</day><month>1</month><year>2017</year></date><date date-type="accepted"><day>24</day><month>2</month><year>2017</year></date></history><permissions><copyright-statement>© The Author(s) 2017</copyright-statement><license license-type="OpenAccess"><license-p>This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">http://creativecommons.org/licenses/by/4.0/</ext-link></license-p></license></permissions><abstract id="Abs1"><p id="Par1">The envelope spike (S) proteins of MERS-CoV and SARS-CoV determine the virus host tropism and entry into host cells, and constitute a promising target for the development of prophylactics and therapeutics. Here, we present high-resolution structures of the trimeric MERS-CoV and SARS-CoV S proteins in its pre-fusion conformation by single particle cryo-electron microscopy. The overall structures resemble that from other coronaviruses including HKU1, MHV and NL63 reported recently, with the exception of the receptor binding domain (RBD). We captured two states of the RBD with receptor binding region either buried (lying state) or exposed (standing state), demonstrating an inherently flexible RBD readily recognized by the receptor. Further sequence conservation analysis of six human-infecting coronaviruses revealed that the fusion peptide, HR1 region and the central helix are potential targets for eliciting broadly neutralizing antibodies.</p><sec><title>Supplementary information</title><p>The online version of this article (doi:10.1038/ncomms15092) contains supplementary material, which is available to authorized users.</p></sec></abstract><abstract id="Abs2" abstract-type="web-summary"><p id="Par2">Host tropism and cell entry of pathogenic coronaviruses are mediated by their envelope spike (S) proteins. Here the authors present structural analyses of trimeric MERS-CoV and SARS-CoV S proteins in pre-fusion conformation, and reveal two states of the receptor binding domain that suggest new avenues for the generation of neutralizing antibodies.</p><sec><title>Supplementary information</title><p>The online version of this article (doi:10.1038/ncomms15092) contains supplementary material, which is available to authorized users.</p></sec></abstract><kwd-group kwd-group-type="npg-subject"><title>Subject terms</title><kwd>Virus structures</kwd><kwd>Cryoelectron microscopy</kwd><kwd>Applied immunology</kwd></kwd-group><custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name><meta-value>© The Author(s) 2017</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Tianjin</li></settlement></list><tree><noCountry><name sortKey="Lu, Guangwen" sort="Lu, Guangwen" uniqKey="Lu G" first="Guangwen" last="Lu">Guangwen Lu</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Yuan, Yuan" sort="Yuan, Yuan" uniqKey="Yuan Y" first="Yuan" last="Yuan">Yuan Yuan</name></noRegion><name sortKey="Cao, Duanfang" sort="Cao, Duanfang" uniqKey="Cao D" first="Duanfang" last="Cao">Duanfang Cao</name><name sortKey="Gao, George F" sort="Gao, George F" uniqKey="Gao G" first="George F." last="Gao">George F. Gao</name><name sortKey="Gao, George F" sort="Gao, George F" uniqKey="Gao G" first="George F." last="Gao">George F. Gao</name><name sortKey="Gao, George F" sort="Gao, George F" uniqKey="Gao G" first="George F." last="Gao">George F. Gao</name><name sortKey="Gao, George F" sort="Gao, George F" uniqKey="Gao G" first="George F." last="Gao">George F. Gao</name><name sortKey="Gao, George F" sort="Gao, George F" uniqKey="Gao G" first="George F." last="Gao">George F. Gao</name><name sortKey="Gao, George F" sort="Gao, George F" uniqKey="Gao G" first="George F." last="Gao">George F. Gao</name><name sortKey="Ma, Jun" sort="Ma, Jun" uniqKey="Ma J" first="Jun" last="Ma">Jun Ma</name><name sortKey="Qi, Jianxun" sort="Qi, Jianxun" uniqKey="Qi J" first="Jianxun" last="Qi">Jianxun Qi</name><name sortKey="Shi, Yi" sort="Shi, Yi" uniqKey="Shi Y" first="Yi" last="Shi">Yi Shi</name><name sortKey="Shi, Yi" sort="Shi, Yi" uniqKey="Shi Y" first="Yi" last="Shi">Yi Shi</name><name sortKey="Shi, Yi" sort="Shi, Yi" uniqKey="Shi Y" first="Yi" last="Shi">Yi Shi</name><name sortKey="Shi, Yi" sort="Shi, Yi" uniqKey="Shi Y" first="Yi" last="Shi">Yi Shi</name><name sortKey="Wang, Qihui" sort="Wang, Qihui" uniqKey="Wang Q" first="Qihui" last="Wang">Qihui Wang</name><name sortKey="Wu, Ying" sort="Wu, Ying" uniqKey="Wu Y" first="Ying" last="Wu">Ying Wu</name><name sortKey="Yan, Jinghua" sort="Yan, Jinghua" uniqKey="Yan J" first="Jinghua" last="Yan">Jinghua Yan</name><name sortKey="Yan, Jinghua" sort="Yan, Jinghua" uniqKey="Yan J" first="Jinghua" last="Yan">Jinghua Yan</name><name sortKey="Yan, Jinghua" sort="Yan, Jinghua" uniqKey="Yan J" first="Jinghua" last="Yan">Jinghua Yan</name><name sortKey="Yan, Jinghua" sort="Yan, Jinghua" uniqKey="Yan J" first="Jinghua" last="Yan">Jinghua Yan</name><name sortKey="Zhang, Xinzheng" sort="Zhang, Xinzheng" uniqKey="Zhang X" first="Xinzheng" last="Zhang">Xinzheng Zhang</name><name sortKey="Zhang, Xinzheng" sort="Zhang, Xinzheng" uniqKey="Zhang X" first="Xinzheng" last="Zhang">Xinzheng Zhang</name><name sortKey="Zhang, Yanfang" sort="Zhang, Yanfang" uniqKey="Zhang Y" first="Yanfang" last="Zhang">Yanfang Zhang</name><name sortKey="Zhang, Yanfang" sort="Zhang, Yanfang" uniqKey="Zhang Y" first="Yanfang" last="Zhang">Yanfang Zhang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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