Establishment and validation of a pseudovirus neutralization assay for SARS-CoV-2
Identifieur interne : 000261 ( Pmc/Checkpoint ); précédent : 000260; suivant : 000262Establishment and validation of a pseudovirus neutralization assay for SARS-CoV-2
Auteurs : Jianhui Nie [République populaire de Chine] ; Qianqian Li [République populaire de Chine] ; Jiajing Wu [République populaire de Chine] ; Chenyan Zhao [République populaire de Chine] ; Huan Hao [République populaire de Chine] ; Huan Liu [République populaire de Chine] ; Li Zhang [République populaire de Chine] ; Lingling Nie [République populaire de Chine] ; Haiyang Qin [République populaire de Chine] ; Meng Wang [République populaire de Chine] ; Qiong Lu [République populaire de Chine] ; Xiaoyu Li [République populaire de Chine] ; Qiyu Sun [République populaire de Chine] ; Junkai Liu [République populaire de Chine] ; Changfa Fan [République populaire de Chine] ; Weijin Huang [République populaire de Chine] ; Miao Xu [République populaire de Chine] ; Youchun Wang [République populaire de Chine]Source :
- Emerging Microbes & Infections [ 2222-1751 ] ; 2020.
Abstract
Pseudoviruses are useful virological tools because of their safety and versatility, especially for emerging and re-emerging viruses. Due to its high pathogenicity and infectivity and the lack of effective vaccines and therapeutics, live SARS-CoV-2 has to be handled under biosafety level 3 conditions, which has hindered the development of vaccines and therapeutics. Based on a VSV pseudovirus production system, a pseudovirus-based neutralization assay has been developed for evaluating neutralizing antibodies against SARS-CoV-2 in biosafety level 2 facilities. The key parameters for this assay were optimized, including cell types, cell numbers, virus inoculum. When tested against the SARS-CoV-2 pseudovirus, SARS-CoV-2 convalescent patient sera showed high neutralizing potency, which underscore its potential as therapeutics. The limit of detection for this assay was determined as 22.1 and 43.2 for human and mouse serum samples respectively using a panel of 120 negative samples. The cutoff values were set as 30 and 50 for human and mouse serum samples, respectively. This assay showed relatively low coefficient of variations with 15.9% and 16.2% for the intra- and inter-assay analyses respectively. Taken together, we established a robust pseudovirus-based neutralization assay for SARS-CoV-2 and are glad to share pseudoviruses and related protocols with the developers of vaccines or therapeutics to fight against this lethal virus.
Url:
DOI: 10.1080/22221751.2020.1743767
PubMed: 32207377
PubMed Central: 7144318
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
PMC:7144318Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Establishment and validation of a pseudovirus neutralization assay for SARS-CoV-2</title><author><name sortKey="Nie, Jianhui" sort="Nie, Jianhui" uniqKey="Nie J" first="Jianhui" last="Nie">Jianhui Nie</name><affiliation wicri:level="1"><nlm:aff id="AF1"><institution>Division of HIV/AIDS and Sex-transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC)</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Li, Qianqian" sort="Li, Qianqian" uniqKey="Li Q" first="Qianqian" last="Li">Qianqian Li</name><affiliation wicri:level="1"><nlm:aff id="AF1"><institution>Division of HIV/AIDS and Sex-transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC)</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="AF2"><institution>Graduate School of Peking Union Medical College</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Wu, Jiajing" sort="Wu, Jiajing" uniqKey="Wu J" first="Jiajing" last="Wu">Jiajing Wu</name><affiliation wicri:level="1"><nlm:aff id="AF1"><institution>Division of HIV/AIDS and Sex-transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC)</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="AF3"><institution>Wuhan Institute of Biological Products</institution>, Wuhan,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Zhao, Chenyan" sort="Zhao, Chenyan" uniqKey="Zhao C" first="Chenyan" last="Zhao">Chenyan Zhao</name><affiliation wicri:level="1"><nlm:aff id="AF1"><institution>Division of HIV/AIDS and Sex-transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC)</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Hao, Huan" sort="Hao, Huan" uniqKey="Hao H" first="Huan" last="Hao">Huan Hao</name><affiliation wicri:level="1"><nlm:aff id="AF1"><institution>Division of HIV/AIDS and Sex-transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC)</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Liu, Huan" sort="Liu, Huan" uniqKey="Liu H" first="Huan" last="Liu">Huan Liu</name><affiliation wicri:level="1"><nlm:aff id="AF1"><institution>Division of HIV/AIDS and Sex-transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC)</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Zhang, Li" sort="Zhang, Li" uniqKey="Zhang L" first="Li" last="Zhang">Li Zhang</name><affiliation wicri:level="1"><nlm:aff id="AF1"><institution>Division of HIV/AIDS and Sex-transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC)</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Nie, Lingling" sort="Nie, Lingling" uniqKey="Nie L" first="Lingling" last="Nie">Lingling Nie</name><affiliation wicri:level="1"><nlm:aff id="AF1"><institution>Division of HIV/AIDS and Sex-transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC)</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Qin, Haiyang" sort="Qin, Haiyang" uniqKey="Qin H" first="Haiyang" last="Qin">Haiyang Qin</name><affiliation wicri:level="1"><nlm:aff id="AF1"><institution>Division of HIV/AIDS and Sex-transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC)</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Wang, Meng" sort="Wang, Meng" uniqKey="Wang M" first="Meng" last="Wang">Meng Wang</name><affiliation wicri:level="1"><nlm:aff id="AF1"><institution>Division of HIV/AIDS and Sex-transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC)</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Lu, Qiong" sort="Lu, Qiong" uniqKey="Lu Q" first="Qiong" last="Lu">Qiong Lu</name><affiliation wicri:level="1"><nlm:aff id="AF1"><institution>Division of HIV/AIDS and Sex-transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC)</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Li, Xiaoyu" sort="Li, Xiaoyu" uniqKey="Li X" first="Xiaoyu" last="Li">Xiaoyu Li</name><affiliation wicri:level="1"><nlm:aff id="AF1"><institution>Division of HIV/AIDS and Sex-transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC)</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Sun, Qiyu" sort="Sun, Qiyu" uniqKey="Sun Q" first="Qiyu" last="Sun">Qiyu Sun</name><affiliation wicri:level="1"><nlm:aff id="AF1"><institution>Division of HIV/AIDS and Sex-transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC)</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Liu, Junkai" sort="Liu, Junkai" uniqKey="Liu J" first="Junkai" last="Liu">Junkai Liu</name><affiliation wicri:level="1"><nlm:aff id="AF1"><institution>Division of HIV/AIDS and Sex-transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC)</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Fan, Changfa" sort="Fan, Changfa" uniqKey="Fan C" first="Changfa" last="Fan">Changfa Fan</name><affiliation wicri:level="1"><nlm:aff id="AF4"><institution>Division of Animal Model Research, Institute for Laboratory Animal Resources, National Institutes for Food and Drug Control</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Huang, Weijin" sort="Huang, Weijin" uniqKey="Huang W" first="Weijin" last="Huang">Weijin Huang</name><affiliation wicri:level="1"><nlm:aff id="AF1"><institution>Division of HIV/AIDS and Sex-transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC)</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Xu, Miao" sort="Xu, Miao" uniqKey="Xu M" first="Miao" last="Xu">Miao Xu</name><affiliation wicri:level="1"><nlm:aff id="AF5"><institution>Institute for Biological Product Control, National Institutes for Food and Drug Control</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Wang, Youchun" sort="Wang, Youchun" uniqKey="Wang Y" first="Youchun" last="Wang">Youchun Wang</name><affiliation wicri:level="1"><nlm:aff id="AF1"><institution>Division of HIV/AIDS and Sex-transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC)</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="AF2"><institution>Graduate School of Peking Union Medical College</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">32207377</idno><idno type="pmc">7144318</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7144318</idno><idno type="RBID">PMC:7144318</idno><idno type="doi">10.1080/22221751.2020.1743767</idno><date when="2020">2020</date><idno type="wicri:Area/Pmc/Corpus">000F84</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000F84</idno><idno type="wicri:Area/Pmc/Curation">000F84</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000F84</idno><idno type="wicri:Area/Pmc/Checkpoint">000261</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000261</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Establishment and validation of a pseudovirus neutralization assay for SARS-CoV-2</title><author><name sortKey="Nie, Jianhui" sort="Nie, Jianhui" uniqKey="Nie J" first="Jianhui" last="Nie">Jianhui Nie</name><affiliation wicri:level="1"><nlm:aff id="AF1"><institution>Division of HIV/AIDS and Sex-transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC)</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Li, Qianqian" sort="Li, Qianqian" uniqKey="Li Q" first="Qianqian" last="Li">Qianqian Li</name><affiliation wicri:level="1"><nlm:aff id="AF1"><institution>Division of HIV/AIDS and Sex-transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC)</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="AF2"><institution>Graduate School of Peking Union Medical College</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Wu, Jiajing" sort="Wu, Jiajing" uniqKey="Wu J" first="Jiajing" last="Wu">Jiajing Wu</name><affiliation wicri:level="1"><nlm:aff id="AF1"><institution>Division of HIV/AIDS and Sex-transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC)</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="AF3"><institution>Wuhan Institute of Biological Products</institution>, Wuhan,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Zhao, Chenyan" sort="Zhao, Chenyan" uniqKey="Zhao C" first="Chenyan" last="Zhao">Chenyan Zhao</name><affiliation wicri:level="1"><nlm:aff id="AF1"><institution>Division of HIV/AIDS and Sex-transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC)</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Hao, Huan" sort="Hao, Huan" uniqKey="Hao H" first="Huan" last="Hao">Huan Hao</name><affiliation wicri:level="1"><nlm:aff id="AF1"><institution>Division of HIV/AIDS and Sex-transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC)</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Liu, Huan" sort="Liu, Huan" uniqKey="Liu H" first="Huan" last="Liu">Huan Liu</name><affiliation wicri:level="1"><nlm:aff id="AF1"><institution>Division of HIV/AIDS and Sex-transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC)</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Zhang, Li" sort="Zhang, Li" uniqKey="Zhang L" first="Li" last="Zhang">Li Zhang</name><affiliation wicri:level="1"><nlm:aff id="AF1"><institution>Division of HIV/AIDS and Sex-transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC)</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Nie, Lingling" sort="Nie, Lingling" uniqKey="Nie L" first="Lingling" last="Nie">Lingling Nie</name><affiliation wicri:level="1"><nlm:aff id="AF1"><institution>Division of HIV/AIDS and Sex-transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC)</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Qin, Haiyang" sort="Qin, Haiyang" uniqKey="Qin H" first="Haiyang" last="Qin">Haiyang Qin</name><affiliation wicri:level="1"><nlm:aff id="AF1"><institution>Division of HIV/AIDS and Sex-transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC)</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Wang, Meng" sort="Wang, Meng" uniqKey="Wang M" first="Meng" last="Wang">Meng Wang</name><affiliation wicri:level="1"><nlm:aff id="AF1"><institution>Division of HIV/AIDS and Sex-transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC)</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Lu, Qiong" sort="Lu, Qiong" uniqKey="Lu Q" first="Qiong" last="Lu">Qiong Lu</name><affiliation wicri:level="1"><nlm:aff id="AF1"><institution>Division of HIV/AIDS and Sex-transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC)</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Li, Xiaoyu" sort="Li, Xiaoyu" uniqKey="Li X" first="Xiaoyu" last="Li">Xiaoyu Li</name><affiliation wicri:level="1"><nlm:aff id="AF1"><institution>Division of HIV/AIDS and Sex-transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC)</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Sun, Qiyu" sort="Sun, Qiyu" uniqKey="Sun Q" first="Qiyu" last="Sun">Qiyu Sun</name><affiliation wicri:level="1"><nlm:aff id="AF1"><institution>Division of HIV/AIDS and Sex-transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC)</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Liu, Junkai" sort="Liu, Junkai" uniqKey="Liu J" first="Junkai" last="Liu">Junkai Liu</name><affiliation wicri:level="1"><nlm:aff id="AF1"><institution>Division of HIV/AIDS and Sex-transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC)</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Fan, Changfa" sort="Fan, Changfa" uniqKey="Fan C" first="Changfa" last="Fan">Changfa Fan</name><affiliation wicri:level="1"><nlm:aff id="AF4"><institution>Division of Animal Model Research, Institute for Laboratory Animal Resources, National Institutes for Food and Drug Control</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Huang, Weijin" sort="Huang, Weijin" uniqKey="Huang W" first="Weijin" last="Huang">Weijin Huang</name><affiliation wicri:level="1"><nlm:aff id="AF1"><institution>Division of HIV/AIDS and Sex-transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC)</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Xu, Miao" sort="Xu, Miao" uniqKey="Xu M" first="Miao" last="Xu">Miao Xu</name><affiliation wicri:level="1"><nlm:aff id="AF5"><institution>Institute for Biological Product Control, National Institutes for Food and Drug Control</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Wang, Youchun" sort="Wang, Youchun" uniqKey="Wang Y" first="Youchun" last="Wang">Youchun Wang</name><affiliation wicri:level="1"><nlm:aff id="AF1"><institution>Division of HIV/AIDS and Sex-transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC)</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="AF2"><institution>Graduate School of Peking Union Medical College</institution>, Beijing,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author></analytic><series><title level="j">Emerging Microbes & Infections</title><idno type="eISSN">2222-1751</idno><imprint><date when="2020">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>ABSTRACT</title><p>Pseudoviruses are useful virological tools because of their safety and versatility, especially for emerging and re-emerging viruses. Due to its high pathogenicity and infectivity and the lack of effective vaccines and therapeutics, live SARS-CoV-2 has to be handled under biosafety level 3 conditions, which has hindered the development of vaccines and therapeutics. Based on a VSV pseudovirus production system, a pseudovirus-based neutralization assay has been developed for evaluating neutralizing antibodies against SARS-CoV-2 in biosafety level 2 facilities. The key parameters for this assay were optimized, including cell types, cell numbers, virus inoculum. When tested against the SARS-CoV-2 pseudovirus, SARS-CoV-2 convalescent patient sera showed high neutralizing potency, which underscore its potential as therapeutics. The limit of detection for this assay was determined as 22.1 and 43.2 for human and mouse serum samples respectively using a panel of 120 negative samples. The cutoff values were set as 30 and 50 for human and mouse serum samples, respectively. This assay showed relatively low coefficient of variations with 15.9% and 16.2% for the intra- and inter-assay analyses respectively. Taken together, we established a robust pseudovirus-based neutralization assay for SARS-CoV-2 and are glad to share pseudoviruses and related protocols with the developers of vaccines or therapeutics to fight against this lethal virus.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Hui Ds, Eia" uniqKey="Hui Ds E">EIA Hui DS</name></author><author><name sortKey="Madani, Ta" uniqKey="Madani T">TA Madani</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, Q" uniqKey="Li Q">Q Li</name></author><author><name sortKey="Guan, X" uniqKey="Guan X">X Guan</name></author><author><name sortKey="Wu, P" uniqKey="Wu P">P Wu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Phelan, Al" uniqKey="Phelan A">AL Phelan</name></author><author><name sortKey="Katz, R" uniqKey="Katz R">R Katz</name></author><author><name sortKey="Gostin, Lo" uniqKey="Gostin L">LO. Gostin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhu, N" uniqKey="Zhu N">N Zhu</name></author><author><name sortKey="Zhang, D" uniqKey="Zhang D">D Zhang</name></author><author><name sortKey="Wang, W" uniqKey="Wang W">W Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chan, Jf" uniqKey="Chan J">JF Chan</name></author><author><name sortKey="Kok, Kh" uniqKey="Kok K">KH Kok</name></author><author><name sortKey="Zhu, Z" uniqKey="Zhu Z">Z Zhu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, Y" uniqKey="Chen Y">Y Chen</name></author><author><name sortKey="Liu, Q" uniqKey="Liu Q">Q Liu</name></author><author><name sortKey="Guo, D" uniqKey="Guo D">D. Guo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wu, A" uniqKey="Wu A">A Wu</name></author><author><name sortKey="Peng, Y" uniqKey="Peng Y">Y Peng</name></author><author><name sortKey="Huang, B" uniqKey="Huang B">B Huang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jiang, S" uniqKey="Jiang S">S Jiang</name></author><author><name sortKey="He, Y" uniqKey="He Y">Y He</name></author><author><name sortKey="Liu, S" uniqKey="Liu S">S. Liu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Du, L" uniqKey="Du L">L Du</name></author><author><name sortKey="He, Y" uniqKey="He Y">Y He</name></author><author><name sortKey="Zhou, Y" uniqKey="Zhou Y">Y Zhou</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Roper, Rl" uniqKey="Roper R">RL Roper</name></author><author><name sortKey="Rehm, Ke" uniqKey="Rehm K">KE. Rehm</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Enjuanes, L" uniqKey="Enjuanes L">L Enjuanes</name></author><author><name sortKey="Zuniga, S" uniqKey="Zuniga S">S Zuniga</name></author><author><name sortKey="Castano Rodriguez, C" uniqKey="Castano Rodriguez C">C Castano-Rodriguez</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fan, C" uniqKey="Fan C">C Fan</name></author><author><name sortKey="Wu, X" uniqKey="Wu X">X Wu</name></author><author><name sortKey="Liu, Q" uniqKey="Liu Q">Q Liu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nie, J" uniqKey="Nie J">J Nie</name></author><author><name sortKey="Wu, X" uniqKey="Wu X">X Wu</name></author><author><name sortKey="Ma, J" uniqKey="Ma J">J Ma</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Liu, Q" uniqKey="Liu Q">Q Liu</name></author><author><name sortKey="Fan, C" uniqKey="Fan C">C Fan</name></author><author><name sortKey="Li, Q" uniqKey="Li Q">Q Li</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, L" uniqKey="Zhang L">L Zhang</name></author><author><name sortKey="Li, Q" uniqKey="Li Q">Q Li</name></author><author><name sortKey="Liu, Q" uniqKey="Liu Q">Q Liu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, Q" uniqKey="Li Q">Q Li</name></author><author><name sortKey="Liu, Q" uniqKey="Liu Q">Q Liu</name></author><author><name sortKey="Huang, W" uniqKey="Huang W">W Huang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wu, J" uniqKey="Wu J">J Wu</name></author><author><name sortKey="Zhao, C" uniqKey="Zhao C">C Zhao</name></author><author><name sortKey="Liu, Q" uniqKey="Liu Q">Q Liu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nie, J" uniqKey="Nie J">J Nie</name></author><author><name sortKey="Liu, L" uniqKey="Liu L">L Liu</name></author><author><name sortKey="Wang, Q" uniqKey="Wang Q">Q Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ma, J" uniqKey="Ma J">J Ma</name></author><author><name sortKey="Chen, R" uniqKey="Chen R">R Chen</name></author><author><name sortKey="Huang, W" uniqKey="Huang W">W Huang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Whitt, Ma" uniqKey="Whitt M">MA. Whitt</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, Q" uniqKey="Chen Q">Q Chen</name></author><author><name sortKey="Nie, J" uniqKey="Nie J">J Nie</name></author><author><name sortKey="Huang, W" uniqKey="Huang W">W Huang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhou, P" uniqKey="Zhou P">P Zhou</name></author><author><name sortKey="Yang, Xl" uniqKey="Yang X">XL Yang</name></author><author><name sortKey="Wang, Xg" uniqKey="Wang X">XG Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kuo, Cc" uniqKey="Kuo C">CC Kuo</name></author><author><name sortKey="Chiang, Aw" uniqKey="Chiang A">AW Chiang</name></author><author><name sortKey="Shamie, I" uniqKey="Shamie I">I Shamie</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nakamura, K" uniqKey="Nakamura K">K Nakamura</name></author><author><name sortKey="Harada, Y" uniqKey="Harada Y">Y Harada</name></author><author><name sortKey="Takahashi, H" uniqKey="Takahashi H">H Takahashi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hofmann, H" uniqKey="Hofmann H">H Hofmann</name></author><author><name sortKey="Hattermann, K" uniqKey="Hattermann K">K Hattermann</name></author><author><name sortKey="Marzi, A" uniqKey="Marzi A">A Marzi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, Q" uniqKey="Li Q">Q Li</name></author><author><name sortKey="Liu, Q" uniqKey="Liu Q">Q Liu</name></author><author><name sortKey="Huang, W" uniqKey="Huang W">W Huang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nie, J" uniqKey="Nie J">J Nie</name></author><author><name sortKey="Huang, W" uniqKey="Huang W">W Huang</name></author><author><name sortKey="Liu, Q" uniqKey="Liu Q">Q Liu</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Emerg Microbes Infect</journal-id><journal-id journal-id-type="iso-abbrev">Emerg Microbes Infect</journal-id><journal-id journal-id-type="publisher-id">TEMI</journal-id><journal-id journal-id-type="publisher-id">temi20</journal-id><journal-title-group><journal-title>Emerging Microbes & Infections</journal-title></journal-title-group><issn pub-type="epub">2222-1751</issn><publisher><publisher-name>Taylor & Francis</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">32207377</article-id><article-id pub-id-type="pmc">7144318</article-id><article-id pub-id-type="publisher-id">1743767</article-id><article-id pub-id-type="doi">10.1080/22221751.2020.1743767</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Establishment and validation of a pseudovirus neutralization assay for SARS-CoV-2</article-title><alt-title alt-title-type="running-title">Emerging Microbes & Infections</alt-title><alt-title alt-title-type="running-authors">J. Nie et al.</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Nie</surname><given-names>Jianhui</given-names></name><xref ref-type="aff" rid="AF1"><sup>a</sup></xref><xref ref-type="author-notes" rid="an1"><sup>*</sup></xref></contrib><contrib contrib-type="author"><name><surname>Li</surname><given-names>Qianqian</given-names></name><xref ref-type="aff" rid="AF1"><sup>a</sup></xref><xref ref-type="aff" rid="AF2"><sup>b</sup></xref><xref ref-type="author-notes" rid="an1"><sup>*</sup></xref></contrib><contrib contrib-type="author"><name><surname>Wu</surname><given-names>Jiajing</given-names></name><xref ref-type="aff" rid="AF1"><sup>a</sup></xref><xref ref-type="aff" rid="AF3"><sup>c</sup></xref><xref ref-type="author-notes" rid="an1"><sup>*</sup></xref></contrib><contrib contrib-type="author"><name><surname>Zhao</surname><given-names>Chenyan</given-names></name><xref ref-type="aff" rid="AF1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Hao</surname><given-names>Huan</given-names></name><xref ref-type="aff" rid="AF1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Liu</surname><given-names>Huan</given-names></name><xref ref-type="aff" rid="AF1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Zhang</surname><given-names>Li</given-names></name><xref ref-type="aff" rid="AF1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Nie</surname><given-names>Lingling</given-names></name><xref ref-type="aff" rid="AF1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Qin</surname><given-names>Haiyang</given-names></name><xref ref-type="aff" rid="AF1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Meng</given-names></name><xref ref-type="aff" rid="AF1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Lu</surname><given-names>Qiong</given-names></name><xref ref-type="aff" rid="AF1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Li</surname><given-names>Xiaoyu</given-names></name><xref ref-type="aff" rid="AF1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Sun</surname><given-names>Qiyu</given-names></name><xref ref-type="aff" rid="AF1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Liu</surname><given-names>Junkai</given-names></name><xref ref-type="aff" rid="AF1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Fan</surname><given-names>Changfa</given-names></name><xref ref-type="aff" rid="AF4"><sup>d</sup></xref></contrib><contrib contrib-type="author"><name><surname>Huang</surname><given-names>Weijin</given-names></name><xref ref-type="aff" rid="AF1"><sup>a</sup></xref><xref ref-type="corresp" rid="cor2"></xref></contrib><contrib contrib-type="author"><name><surname>Xu</surname><given-names>Miao</given-names></name><xref ref-type="aff" rid="AF5"><sup>e</sup></xref><xref ref-type="corresp" rid="cor3"></xref></contrib><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Youchun</given-names></name><xref ref-type="aff" rid="AF1"><sup>a</sup></xref><xref ref-type="aff" rid="AF2"><sup>b</sup></xref><xref ref-type="corresp" rid="cor1"><sup>CONTACT</sup></xref></contrib><aff id="AF1"><label>a</label><institution>Division of HIV/AIDS and Sex-transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC)</institution>, Beijing,<country>People’s Republic of China</country></aff><aff id="AF2"><label>b</label><institution>Graduate School of Peking Union Medical College</institution>, Beijing,<country>People’s Republic of China</country></aff><aff id="AF3"><label>c</label><institution>Wuhan Institute of Biological Products</institution>, Wuhan,<country>People’s Republic of China</country></aff><aff id="AF4"><label>d</label><institution>Division of Animal Model Research, Institute for Laboratory Animal Resources, National Institutes for Food and Drug Control</institution>, Beijing,<country>People’s Republic of China</country></aff><aff id="AF5"><label>e</label><institution>Institute for Biological Product Control, National Institutes for Food and Drug Control</institution>, Beijing,<country>People’s Republic of China</country></aff></contrib-group><author-notes><corresp id="cor1"><label>CONTACT</label> Youchun Wang <email xlink:href="wangyc@nifdc.org.cns">wangyc@nifdc.org.cns</email><institution>Division of HIV/AIDS and Sex-transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC)</institution>, <addr-line>No. 31 Huatuo Street, Daxing District, Beijing 102629, People’s Republic of China; Graduate School of Peking Union Medical College, No. 9 Dongdan Santiao, Dongcheng District</addr-line>, Beijing 100730, <country>People’s Republic of China</country></corresp><corresp id="cor2">Weijin Huang <email xlink:href="huangweijin@nifdc.org.cn">huangweijin@nifdc.org.cn</email><institution>Division of HIV/AIDS and Sex-transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC)</institution>, <addr-line>No. 31 Huatuo Street, Daxing District</addr-line>, Beijing 102629, <country>People’s Republic of China</country></corresp><corresp id="cor3">Miao Xu <email xlink:href="xumiaobj@126.com">xumiaobj@126.com</email><institution>Institute for Biological Product Control, National Institutes for Food and Drug Control</institution>, Beijing 100050, <country>People’s Republic of China</country></corresp><fn id="an1"><label>*</label><p>These authors contributed equally to this work.</p></fn></author-notes><pub-date pub-type="collection"><year>2020</year></pub-date><pub-date pub-type="epub"><day>24</day><month>3</month><year>2020</year></pub-date><volume>9</volume><issue>1</issue><fpage seq="76">680</fpage><lpage>686</lpage><history><date date-type="received"><day>24</day><month>2</month><year>2020</year></date><date date-type="rev-recd"><day>10</day><month>3</month><year>2020</year></date><date date-type="accepted"><day>11</day><month>3</month><year>2020</year></date></history><permissions><copyright-statement>© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd</copyright-statement><copyright-year>2020</copyright-year><copyright-holder>The Author(s)</copyright-holder><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by/4.0/"><license-p>This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">http://creativecommons.org/licenses/by/4.0/</ext-link>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p></license></permissions><self-uri content-type="pdf" xlink:href="TEMI_9_1743767.pdf"></self-uri><abstract><title>ABSTRACT</title><p>Pseudoviruses are useful virological tools because of their safety and versatility, especially for emerging and re-emerging viruses. Due to its high pathogenicity and infectivity and the lack of effective vaccines and therapeutics, live SARS-CoV-2 has to be handled under biosafety level 3 conditions, which has hindered the development of vaccines and therapeutics. Based on a VSV pseudovirus production system, a pseudovirus-based neutralization assay has been developed for evaluating neutralizing antibodies against SARS-CoV-2 in biosafety level 2 facilities. The key parameters for this assay were optimized, including cell types, cell numbers, virus inoculum. When tested against the SARS-CoV-2 pseudovirus, SARS-CoV-2 convalescent patient sera showed high neutralizing potency, which underscore its potential as therapeutics. The limit of detection for this assay was determined as 22.1 and 43.2 for human and mouse serum samples respectively using a panel of 120 negative samples. The cutoff values were set as 30 and 50 for human and mouse serum samples, respectively. This assay showed relatively low coefficient of variations with 15.9% and 16.2% for the intra- and inter-assay analyses respectively. Taken together, we established a robust pseudovirus-based neutralization assay for SARS-CoV-2 and are glad to share pseudoviruses and related protocols with the developers of vaccines or therapeutics to fight against this lethal virus.</p></abstract><kwd-group kwd-group-type="author"><title>KEYWORDS</title><kwd>SARS-CoV-2</kwd><kwd>COVID-19</kwd><kwd>neutralizing antibody</kwd><kwd>pseudovirus</kwd><kwd>neutralization assay</kwd></kwd-group><funding-group><award-group><funding-source><named-content content-type="funder-name">National Science and Technology Major Projects of Drug Discovery</named-content></funding-source><award-id>2018ZX09101001</award-id></award-group><funding-statement>This work was supported by National Science and Technology Major Projects of Drug Discovery [grant number 2018ZX09101001].</funding-statement></funding-group><counts><fig-count count="5"></fig-count><table-count count="0"></table-count><equation-count count="0"></equation-count><ref-count count="27"></ref-count><page-count count="7"></page-count></counts></article-meta></front></pmc><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Nie, Jianhui" sort="Nie, Jianhui" uniqKey="Nie J" first="Jianhui" last="Nie">Jianhui Nie</name></noRegion><name sortKey="Fan, Changfa" sort="Fan, Changfa" uniqKey="Fan C" first="Changfa" last="Fan">Changfa Fan</name><name sortKey="Hao, Huan" sort="Hao, Huan" uniqKey="Hao H" first="Huan" last="Hao">Huan Hao</name><name sortKey="Huang, Weijin" sort="Huang, Weijin" uniqKey="Huang W" first="Weijin" last="Huang">Weijin Huang</name><name sortKey="Li, Qianqian" sort="Li, Qianqian" uniqKey="Li Q" first="Qianqian" last="Li">Qianqian Li</name><name sortKey="Li, Qianqian" sort="Li, Qianqian" uniqKey="Li Q" first="Qianqian" last="Li">Qianqian Li</name><name sortKey="Li, Xiaoyu" sort="Li, Xiaoyu" uniqKey="Li X" first="Xiaoyu" last="Li">Xiaoyu Li</name><name sortKey="Liu, Huan" sort="Liu, Huan" uniqKey="Liu H" first="Huan" last="Liu">Huan Liu</name><name sortKey="Liu, Junkai" sort="Liu, Junkai" uniqKey="Liu J" first="Junkai" last="Liu">Junkai Liu</name><name sortKey="Lu, Qiong" sort="Lu, Qiong" uniqKey="Lu Q" first="Qiong" last="Lu">Qiong Lu</name><name sortKey="Nie, Lingling" sort="Nie, Lingling" uniqKey="Nie L" first="Lingling" last="Nie">Lingling Nie</name><name sortKey="Qin, Haiyang" sort="Qin, Haiyang" uniqKey="Qin H" first="Haiyang" last="Qin">Haiyang Qin</name><name sortKey="Sun, Qiyu" sort="Sun, Qiyu" uniqKey="Sun Q" first="Qiyu" last="Sun">Qiyu Sun</name><name sortKey="Wang, Meng" sort="Wang, Meng" uniqKey="Wang M" first="Meng" last="Wang">Meng Wang</name><name sortKey="Wang, Youchun" sort="Wang, Youchun" uniqKey="Wang Y" first="Youchun" last="Wang">Youchun Wang</name><name sortKey="Wang, Youchun" sort="Wang, Youchun" uniqKey="Wang Y" first="Youchun" last="Wang">Youchun Wang</name><name sortKey="Wu, Jiajing" sort="Wu, Jiajing" uniqKey="Wu J" first="Jiajing" last="Wu">Jiajing Wu</name><name sortKey="Wu, Jiajing" sort="Wu, Jiajing" uniqKey="Wu J" first="Jiajing" last="Wu">Jiajing Wu</name><name sortKey="Xu, Miao" sort="Xu, Miao" uniqKey="Xu M" first="Miao" last="Xu">Miao Xu</name><name sortKey="Zhang, Li" sort="Zhang, Li" uniqKey="Zhang L" first="Li" last="Zhang">Li Zhang</name><name sortKey="Zhao, Chenyan" sort="Zhao, Chenyan" uniqKey="Zhao C" first="Chenyan" last="Zhao">Chenyan Zhao</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Pmc/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000261 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd -nk 000261 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Pmc
|étape= Checkpoint
|type= RBID
|clé= PMC:7144318
|texte= Establishment and validation of a pseudovirus neutralization assay for SARS-CoV-2
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/RBID.i -Sk "pubmed:32207377" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |