SrasV1, PascalFrancis, Curation, bibRecord, 000877

Development of a molecular-beacon-based multi-allelic real-time RT-PCR assay for the detection of human coronavirus causing severe acute respiratory syndrome (SARS-CoV): a general methodology for detecting rapidly mutating viruses

Identifieur interne : 000877 ( PascalFrancis/Curation ); précédent : 000876; suivant : 000878

Development of a molecular-beacon-based multi-allelic real-time RT-PCR assay for the detection of human coronavirus causing severe acute respiratory syndrome (SARS-CoV): a general methodology for detecting rapidly mutating viruses

Auteurs : Andreas V. Hadjinicolaou [Chypre (pays)] ; Gabriella A. Farcas [Canada] ; Victoria L. Demetriou [Chypre (pays)] ; Tony Mazzulli [Canada] ; Susan M. Poutanen [Canada] ; Barbara M. Willey [Canada] ; Donald E. Low [Canada] ; Jagdish Butany [Canada] ; Sylvia L. Asa [Canada] ; Kevin C. Kain [Canada] ; Leondios G. Kostrikis [Chypre (pays)]

Source :

Archives of virology [ 0304-8608 ] ; 2011.

RBID : Pascal:11-0216070

Descripteurs français

Pascal (Inist)
- Homme, Coronavirus, Virus syndrome respiratoire aigu sévère, Temps réel, Réaction chaîne polymérase RT, Détection, Syndrome respiratoire aigu sévère.
Wicri :
- topic : Homme.

English descriptors

KwdEn :
- Coronavirus, Detection, Human, Real time, Reverse transcription polymerase chain reaction, Severe acute respiratory syndrome, Severe acute respiratory syndrome virus.

Abstract

Emerging infectious diseases have caused a global effort for development of fast and accurate detection techniques. The rapidly mutating nature of viruses presents a major difficulty, highlighting the need for specific detection of genetically diverse strains. One such infectious agent is SARS-associated coronavirus (SARS-CoV), which emerged in 2003. This study aimed to develop a real-time RT-PCR detection assay specific for SARS-CoV, taking into account its intrinsic polymorphic nature due to genetic drift and recombination and the possibility of continuous and multiple introductions of genetically non-identical strains into the human population, by using mismatch-tolerant molecular beacons designed to specifically detect the SARS-CoV S, E, M and N genes. These were applied in simple, reproducible duplex and multiplex real-time PCR assays on 25 post-mortem samples and constructed RNA controls, and they demonstrated high target detection ability and specificity. This assay can readily be adapted for detection of other emerging and rapidly mutating pathogens.

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A11	`01`	`1`		`@1 HADJINICOLAOU (Andreas V.)`
A11	`02`	`1`		`@1 FARCAS (Gabriella A.)`
A11	`03`	`1`		`@1 DEMETRIOU (Victoria L.)`
A11	`04`	`1`		`@1 MAZZULLI (Tony)`
A11	`05`	`1`		`@1 POUTANEN (Susan M.)`
A11	`06`	`1`		`@1 WILLEY (Barbara M.)`
A11	`07`	`1`		`@1 LOW (Donald E.)`
A11	`08`	`1`		`@1 BUTANY (Jagdish)`
A11	`09`	`1`		`@1 ASA (Sylvia L.)`
A11	`10`	`1`		`@1 KAIN (Kevin C.)`
A11	`11`	`1`		`@1 KOSTRIKIS (Leondios G.)`
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C07	`05`	`X`	`FRE`	`@0 Virose`
C07	`05`	`X`	`ENG`	`@0 Viral disease`
C07	`05`	`X`	`SPA`	`@0 Virosis`
C07	`06`	`X`	`FRE`	`@0 Infection`
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C07	`07`	`X`	`FRE`	`@0 Pathologie des poumons @5 16`
C07	`07`	`X`	`ENG`	`@0 Lung disease @5 16`
C07	`07`	`X`	`SPA`	`@0 Pulmón patología @5 16`
N21				`@1 143`
N44	`01`			`@1 OTO`
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Pascal:11-0216070

Le document en format XML

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<author><name sortKey="Kostrikis, Leondios G" sort="Kostrikis, Leondios G" uniqKey="Kostrikis L" first="Leondios G." last="Kostrikis">Leondios G. Kostrikis</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Development of a molecular-beacon-based multi-allelic real-time RT-PCR assay for the detection of human coronavirus causing severe acute respiratory syndrome (SARS-CoV): a general methodology for detecting rapidly mutating viruses</title>
<author><name sortKey="Hadjinicolaou, Andreas V" sort="Hadjinicolaou, Andreas V" uniqKey="Hadjinicolaou A" first="Andreas V." last="Hadjinicolaou">Andreas V. Hadjinicolaou</name>
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<author><name sortKey="Kostrikis, Leondios G" sort="Kostrikis, Leondios G" uniqKey="Kostrikis L" first="Leondios G." last="Kostrikis">Leondios G. Kostrikis</name>
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<series><title level="j" type="main">Archives of virology</title>
<title level="j" type="abbreviated">Arch. virol.</title>
<idno type="ISSN">0304-8608</idno>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Coronavirus</term>
<term>Detection</term>
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<term>Real time</term>
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<term>Severe acute respiratory syndrome</term>
<term>Severe acute respiratory syndrome virus</term>
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<front><div type="abstract" xml:lang="en">Emerging infectious diseases have caused a global effort for development of fast and accurate detection techniques. The rapidly mutating nature of viruses presents a major difficulty, highlighting the need for specific detection of genetically diverse strains. One such infectious agent is SARS-associated coronavirus (SARS-CoV), which emerged in 2003. This study aimed to develop a real-time RT-PCR detection assay specific for SARS-CoV, taking into account its intrinsic polymorphic nature due to genetic drift and recombination and the possibility of continuous and multiple introductions of genetically non-identical strains into the human population, by using mismatch-tolerant molecular beacons designed to specifically detect the SARS-CoV S, E, M and N genes. These were applied in simple, reproducible duplex and multiplex real-time PCR assays on 25 post-mortem samples and constructed RNA controls, and they demonstrated high target detection ability and specificity. This assay can readily be adapted for detection of other emerging and rapidly mutating pathogens.</div>
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<fA08 i1="01" i2="1" l="ENG"><s1>Development of a molecular-beacon-based multi-allelic real-time RT-PCR assay for the detection of human coronavirus causing severe acute respiratory syndrome (SARS-CoV): a general methodology for detecting rapidly mutating viruses</s1>
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<fA11 i1="01" i2="1"><s1>HADJINICOLAOU (Andreas V.)</s1>
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<fA11 i1="02" i2="1"><s1>FARCAS (Gabriella A.)</s1>
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<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Pathologie de l'appareil respiratoire</s0>
<s5>13</s5>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Respiratory disease</s0>
<s5>13</s5>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Aparato respiratorio patología</s0>
<s5>13</s5>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Virose</s0>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Viral disease</s0>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Virosis</s0>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Infection</s0>
</fC07>
<fC07 i1="06" i2="X" l="ENG"><s0>Infection</s0>
</fC07>
<fC07 i1="06" i2="X" l="SPA"><s0>Infección</s0>
</fC07>
<fC07 i1="07" i2="X" l="FRE"><s0>Pathologie des poumons</s0>
<s5>16</s5>
</fC07>
<fC07 i1="07" i2="X" l="ENG"><s0>Lung disease</s0>
<s5>16</s5>
</fC07>
<fC07 i1="07" i2="X" l="SPA"><s0>Pulmón patología</s0>
<s5>16</s5>
</fC07>
<fN21><s1>143</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
</standard>
</inist>
</record>

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   |wiki=    Sante
   |area=    SrasV1
   |flux=    PascalFrancis
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   |clé=     Pascal:11-0216070
   |texte=   Development of a molecular-beacon-based multi-allelic real-time RT-PCR assay for the detection of human coronavirus causing severe acute respiratory syndrome (SARS-CoV): a general methodology for detecting rapidly mutating viruses
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Development of a molecular-beacon-based multi-allelic real-time RT-PCR assay for the detection of human coronavirus causing severe acute respiratory syndrome (SARS-CoV): a general methodology for detecting rapidly mutating viruses

Development of a molecular-beacon-based multi-allelic real-time RT-PCR assay for the detection of human coronavirus causing severe acute respiratory syndrome (SARS-CoV): a general methodology for detecting rapidly mutating viruses

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