SrasV1, PascalFrancis, Curation, bibRecord, 000571

Immunogenicity of a receptor-binding domain of SARS coronavirus spike protein in mice : Implications for a subunit vaccine

Identifieur interne : 000571 ( PascalFrancis/Curation ); précédent : 000570; suivant : 000572

Immunogenicity of a receptor-binding domain of SARS coronavirus spike protein in mice : Implications for a subunit vaccine

Auteurs : Alexander N. Zakhartchouk [Canada] ; Chetna Sharon [Canada] ; Malathy Satkunarajah [Canada] ; Thierry Auperin [Canada] ; Sathiyanarayanan Viswanathan [Canada] ; George Mutwiri [Canada] ; Martin Petric [Canada] ; Raymond H. See [Canada] ; Robert C. Brunham [Canada] ; B. Brett Finlay [Canada] ; Cheryl Cameron [Canada] ; David J. Kelvin [Canada] ; Alan Cochrane [Canada] ; James M. Rini [Canada] ; Lorne A. Babiuk [Canada]

Source :

Vaccine [ 0264-410X ] ; 2007.

RBID : Pascal:07-0078280

Descripteurs français

Pascal (Inist)
- Virus syndrome respiratoire aigu sévère, Souris, Immunogénicité, Protéine, Sousunité, Vaccin.
Wicri :
- topic : Vaccin.

English descriptors

KwdEn :
- Immunogenicity, Mouse, Protein, Severe acute respiratory syndrome virus, Subunit, Vaccine.

Abstract

We studied the immunogenicity of an anti-SARS subunit vaccine comprised of the fragment of the SARS coronavirus (SARS-CoV) spike protein amino acids 318-510 (S318-510) containing the receptor-binding domain. The S protein fragment was purified from the culture supernatant of stably transformed HEK293T cells secreting a tagged version of the protein. The vaccine was given subcutaneously to 129S6/SvEv mice in saline, with alum adjuvant or with alum plus CpG oligodeoxynucleotides (ODN). Mice immunized with the adjuvanted antigen elicited strong antibody and cellular immune responses; furthermore, adding the CpG ODN to the alum resulted in increased IgG2a antibody titers and a higher number of INF-γ-secreting murine splenocytes. Mice vaccinated with S318-510 deglycosylated by PNGase F (dgS318-510) showed a lower neutralizing antibody response but had similar numbers of INF-γ-producing cells in the spleen. This finding suggests that carbohydrate is important for the immunogenicity of the S318-510 protein fragment and provide useful information for designing an effective and safe SARS subunit vaccine.

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C01	`01`		`ENG`	@0 We studied the immunogenicity of an anti-SARS subunit vaccine comprised of the fragment of the SARS coronavirus (SARS-CoV) spike protein amino acids 318-510 (S318-510) containing the receptor-binding domain. The S protein fragment was purified from the culture supernatant of stably transformed HEK293T cells secreting a tagged version of the protein. The vaccine was given subcutaneously to 129S6/SvEv mice in saline, with alum adjuvant or with alum plus CpG oligodeoxynucleotides (ODN). Mice immunized with the adjuvanted antigen elicited strong antibody and cellular immune responses; furthermore, adding the CpG ODN to the alum resulted in increased IgG2a antibody titers and a higher number of INF-γ-secreting murine splenocytes. Mice vaccinated with S318-510 deglycosylated by PNGase F (dgS318-510) showed a lower neutralizing antibody response but had similar numbers of INF-γ-producing cells in the spleen. This finding suggests that carbohydrate is important for the immunogenicity of the S318-510 protein fragment and provide useful information for designing an effective and safe SARS subunit vaccine.
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Links toward previous steps (curation, corpus...)

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Links to Exploration step

Pascal:07-0078280

Le document en format XML

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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Immunogenicity of a receptor-binding domain of SARS coronavirus spike protein in mice : Implications for a subunit vaccine</title>
<author><name sortKey="Zakhartchouk, Alexander N" sort="Zakhartchouk, Alexander N" uniqKey="Zakhartchouk A" first="Alexander N." last="Zakhartchouk">Alexander N. Zakhartchouk</name>
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<author><name sortKey="Sharon, Chetna" sort="Sharon, Chetna" uniqKey="Sharon C" first="Chetna" last="Sharon">Chetna Sharon</name>
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<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Biochemistry, University of Toronto</s1>
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<author><name sortKey="Satkunarajah, Malathy" sort="Satkunarajah, Malathy" uniqKey="Satkunarajah M" first="Malathy" last="Satkunarajah">Malathy Satkunarajah</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Departments of Molecular and Medical Genetics and Microbiology, University of Toronto</s1>
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<author><name sortKey="Auperin, Thierry" sort="Auperin, Thierry" uniqKey="Auperin T" first="Thierry" last="Auperin">Thierry Auperin</name>
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<series><title level="j" type="main">Vaccine</title>
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<front><div type="abstract" xml:lang="en">We studied the immunogenicity of an anti-SARS subunit vaccine comprised of the fragment of the SARS coronavirus (SARS-CoV) spike protein amino acids 318-510 (S318-510) containing the receptor-binding domain. The S protein fragment was purified from the culture supernatant of stably transformed HEK293T cells secreting a tagged version of the protein. The vaccine was given subcutaneously to 129S6/SvEv mice in saline, with alum adjuvant or with alum plus CpG oligodeoxynucleotides (ODN). Mice immunized with the adjuvanted antigen elicited strong antibody and cellular immune responses; furthermore, adding the CpG ODN to the alum resulted in increased IgG2a antibody titers and a higher number of INF-γ-secreting murine splenocytes. Mice vaccinated with S318-510 deglycosylated by PNGase F (dgS318-510) showed a lower neutralizing antibody response but had similar numbers of INF-γ-producing cells in the spleen. This finding suggests that carbohydrate is important for the immunogenicity of the S318-510 protein fragment and provide useful information for designing an effective and safe SARS subunit vaccine.</div>
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Immunogenicity of a receptor-binding domain of SARS coronavirus spike protein in mice : Implications for a subunit vaccine

Immunogenicity of a receptor-binding domain of SARS coronavirus spike protein in mice : Implications for a subunit vaccine

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