Serveur d'exploration SRAS

Attention, ce site est en cours de développement !
Attention, site généré par des moyens informatiques à partir de corpus bruts.
Les informations ne sont donc pas validées.

Evaluation of affymetrix severe acute respiratory syndrome resequencing GeneChips in characterization of the genomes of two strains of coronavirus infecting humans

Identifieur interne : 000492 ( PascalFrancis/Curation ); précédent : 000491; suivant : 000493

Evaluation of affymetrix severe acute respiratory syndrome resequencing GeneChips in characterization of the genomes of two strains of coronavirus infecting humans

Auteurs : Irshad M. Sulaiman [États-Unis] ; XIN LIU [États-Unis] ; Michael Frace [États-Unis] ; Nikhat Sulaiman [États-Unis] ; Melissa Olsen-Rasmussen [États-Unis] ; Elizabeth Neuhaus [États-Unis] ; Paul A. Rota [États-Unis] ; Robert M. Wohlhueter [États-Unis]

Source :

RBID : Pascal:06-0260043

Descripteurs français

English descriptors

Abstract

Severe acute respiratory syndrome (SARS) was discovered during a recent global outbreak of atypical pneumonia. A number of immunologic and molecular studies of the clinical samples led to the conclusion that a novel coronavirus (SARS-CoV) was associated with the outbreak. Later, a SARS resequencing GeneChip was developed by Aflymetrix to characterize the complete genome of SARS-CoV on a single GeneChip. The present study was carried out to evaluate the performance of SARS resequencing GeneChips. Two human SARS-CoV strains (CDC#200301157 and Urbani) were resequenced by the SARS GeneChips. Five overlapping PCR amplicons were generated for each strain and hybridized with these GeneChips. The successfully hybridized GeneChips generated nucleotide sequences of nearly complete genomes for the two SARS-CoV strains with an average call rate of 94.6%. Multiple alignments of nucleotide sequences obtained from SARS GeneChips and conventional sequencing revealed full concordance. Furthermore, the GeneChip-based analysis revealed no additional polymorphic sites. The results of this study suggest that GeneChip-based genome characterization is fast and reproducible. Thus, SARS resequencing GeneChips may be employed as an alternate tool to obtain genome sequences of SARS-CoV strains pathogenic for humans in order to further understand the transmission dynamics of these viruses.
pA  
A01 01  1    @0 0099-2240
A02 01      @0 AEMIDF
A03   1    @0 Appl. environ. microbiol. : (Print)
A05       @2 72
A06       @2 1
A08 01  1  ENG  @1 Evaluation of affymetrix severe acute respiratory syndrome resequencing GeneChips in characterization of the genomes of two strains of coronavirus infecting humans
A11 01  1    @1 SULAIMAN (Irshad M.)
A11 02  1    @1 XIN LIU
A11 03  1    @1 FRACE (Michael)
A11 04  1    @1 SULAIMAN (Nikhat)
A11 05  1    @1 OLSEN-RASMUSSEN (Melissa)
A11 06  1    @1 NEUHAUS (Elizabeth)
A11 07  1    @1 ROTA (Paul A.)
A11 08  1    @1 WOHLHUETER (Robert M.)
A14 01      @1 Biotechnology Core Facility Branch, Scientific Resources Program, National Center for Infectious Diseases, Centers for Disease Control and Prevention @2 Atlanta, Georgia 30333 @3 USA @Z 1 aut. @Z 3 aut. @Z 4 aut. @Z 5 aut. @Z 6 aut. @Z 8 aut.
A14 02      @1 Respiratory and Enteric Viruses Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention @2 Atlanta, Georgia 30333 @3 USA @Z 2 aut. @Z 7 aut.
A20       @1 207-211
A21       @1 2006
A23 01      @0 ENG
A43 01      @1 INIST @2 7195 @5 354000133228220250
A44       @0 0000 @1 © 2006 INIST-CNRS. All rights reserved.
A45       @0 15 ref.
A47 01  1    @0 06-0260043
A60       @1 P
A61       @0 A
A64 01  1    @0 Applied and environmental microbiology : (Print)
A66 01      @0 USA
C01 01    ENG  @0 Severe acute respiratory syndrome (SARS) was discovered during a recent global outbreak of atypical pneumonia. A number of immunologic and molecular studies of the clinical samples led to the conclusion that a novel coronavirus (SARS-CoV) was associated with the outbreak. Later, a SARS resequencing GeneChip was developed by Aflymetrix to characterize the complete genome of SARS-CoV on a single GeneChip. The present study was carried out to evaluate the performance of SARS resequencing GeneChips. Two human SARS-CoV strains (CDC#200301157 and Urbani) were resequenced by the SARS GeneChips. Five overlapping PCR amplicons were generated for each strain and hybridized with these GeneChips. The successfully hybridized GeneChips generated nucleotide sequences of nearly complete genomes for the two SARS-CoV strains with an average call rate of 94.6%. Multiple alignments of nucleotide sequences obtained from SARS GeneChips and conventional sequencing revealed full concordance. Furthermore, the GeneChip-based analysis revealed no additional polymorphic sites. The results of this study suggest that GeneChip-based genome characterization is fast and reproducible. Thus, SARS resequencing GeneChips may be employed as an alternate tool to obtain genome sequences of SARS-CoV strains pathogenic for humans in order to further understand the transmission dynamics of these viruses.
C02 01  X    @0 002A05
C03 01  X  FRE  @0 Caractérisation @5 10
C03 01  X  ENG  @0 Characterization @5 10
C03 01  X  SPA  @0 Caracterización @5 10
C03 02  X  FRE  @0 Génome @5 11
C03 02  X  ENG  @0 Genome @5 11
C03 02  X  SPA  @0 Genoma @5 11
C03 03  X  FRE  @0 Homme @5 12
C03 03  X  ENG  @0 Human @5 12
C03 03  X  SPA  @0 Hombre @5 12
C03 04  X  FRE  @0 Coronavirus @2 NW @5 13
C03 04  X  ENG  @0 Coronavirus @2 NW @5 13
C03 04  X  SPA  @0 Coronavirus @2 NW @5 13
C03 05  X  FRE  @0 Syndrome respiratoire aigu sévère @2 NM @5 19
C03 05  X  ENG  @0 Severe acute respiratory syndrome @2 NM @5 19
C03 05  X  SPA  @0 Síndrome respiratorio agudo severo @2 NM @5 19
C07 01  X  FRE  @0 Coronaviridae @2 NW
C07 01  X  ENG  @0 Coronaviridae @2 NW
C07 01  X  SPA  @0 Coronaviridae @2 NW
C07 02  X  FRE  @0 Nidovirales @2 NW
C07 02  X  ENG  @0 Nidovirales @2 NW
C07 02  X  SPA  @0 Nidovirales @2 NW
C07 03  X  FRE  @0 Virus @2 NW
C07 03  X  ENG  @0 Virus @2 NW
C07 03  X  SPA  @0 Virus @2 NW
C07 04  X  FRE  @0 Virose
C07 04  X  ENG  @0 Viral disease
C07 04  X  SPA  @0 Virosis
C07 05  X  FRE  @0 Infection
C07 05  X  ENG  @0 Infection
C07 05  X  SPA  @0 Infección
N21       @1 163

Links toward previous steps (curation, corpus...)


Links to Exploration step

Pascal:06-0260043

Le document en format XML

<record>
<TEI>
<teiHeader>
<fileDesc>
<titleStmt>
<title xml:lang="en" level="a">Evaluation of affymetrix severe acute respiratory syndrome resequencing GeneChips in characterization of the genomes of two strains of coronavirus infecting humans</title>
<author>
<name sortKey="Sulaiman, Irshad M" sort="Sulaiman, Irshad M" uniqKey="Sulaiman I" first="Irshad M." last="Sulaiman">Irshad M. Sulaiman</name>
<affiliation wicri:level="1">
<inist:fA14 i1="01">
<s1>Biotechnology Core Facility Branch, Scientific Resources Program, National Center for Infectious Diseases, Centers for Disease Control and Prevention</s1>
<s2>Atlanta, Georgia 30333</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
</affiliation>
</author>
<author>
<name sortKey="Xin Liu" sort="Xin Liu" uniqKey="Xin Liu" last="Xin Liu">XIN LIU</name>
<affiliation wicri:level="1">
<inist:fA14 i1="02">
<s1>Respiratory and Enteric Viruses Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention</s1>
<s2>Atlanta, Georgia 30333</s2>
<s3>USA</s3>
<sZ>2 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
</affiliation>
</author>
<author>
<name sortKey="Frace, Michael" sort="Frace, Michael" uniqKey="Frace M" first="Michael" last="Frace">Michael Frace</name>
<affiliation wicri:level="1">
<inist:fA14 i1="01">
<s1>Biotechnology Core Facility Branch, Scientific Resources Program, National Center for Infectious Diseases, Centers for Disease Control and Prevention</s1>
<s2>Atlanta, Georgia 30333</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
</affiliation>
</author>
<author>
<name sortKey="Sulaiman, Nikhat" sort="Sulaiman, Nikhat" uniqKey="Sulaiman N" first="Nikhat" last="Sulaiman">Nikhat Sulaiman</name>
<affiliation wicri:level="1">
<inist:fA14 i1="01">
<s1>Biotechnology Core Facility Branch, Scientific Resources Program, National Center for Infectious Diseases, Centers for Disease Control and Prevention</s1>
<s2>Atlanta, Georgia 30333</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
</affiliation>
</author>
<author>
<name sortKey="Olsen Rasmussen, Melissa" sort="Olsen Rasmussen, Melissa" uniqKey="Olsen Rasmussen M" first="Melissa" last="Olsen-Rasmussen">Melissa Olsen-Rasmussen</name>
<affiliation wicri:level="1">
<inist:fA14 i1="01">
<s1>Biotechnology Core Facility Branch, Scientific Resources Program, National Center for Infectious Diseases, Centers for Disease Control and Prevention</s1>
<s2>Atlanta, Georgia 30333</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
</affiliation>
</author>
<author>
<name sortKey="Neuhaus, Elizabeth" sort="Neuhaus, Elizabeth" uniqKey="Neuhaus E" first="Elizabeth" last="Neuhaus">Elizabeth Neuhaus</name>
<affiliation wicri:level="1">
<inist:fA14 i1="01">
<s1>Biotechnology Core Facility Branch, Scientific Resources Program, National Center for Infectious Diseases, Centers for Disease Control and Prevention</s1>
<s2>Atlanta, Georgia 30333</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
</affiliation>
</author>
<author>
<name sortKey="Rota, Paul A" sort="Rota, Paul A" uniqKey="Rota P" first="Paul A." last="Rota">Paul A. Rota</name>
<affiliation wicri:level="1">
<inist:fA14 i1="02">
<s1>Respiratory and Enteric Viruses Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention</s1>
<s2>Atlanta, Georgia 30333</s2>
<s3>USA</s3>
<sZ>2 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
</affiliation>
</author>
<author>
<name sortKey="Wohlhueter, Robert M" sort="Wohlhueter, Robert M" uniqKey="Wohlhueter R" first="Robert M." last="Wohlhueter">Robert M. Wohlhueter</name>
<affiliation wicri:level="1">
<inist:fA14 i1="01">
<s1>Biotechnology Core Facility Branch, Scientific Resources Program, National Center for Infectious Diseases, Centers for Disease Control and Prevention</s1>
<s2>Atlanta, Georgia 30333</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
</affiliation>
</author>
</titleStmt>
<publicationStmt>
<idno type="wicri:source">INIST</idno>
<idno type="inist">06-0260043</idno>
<date when="2006">2006</date>
<idno type="stanalyst">PASCAL 06-0260043 INIST</idno>
<idno type="RBID">Pascal:06-0260043</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000498</idno>
<idno type="wicri:Area/PascalFrancis/Curation">000492</idno>
</publicationStmt>
<sourceDesc>
<biblStruct>
<analytic>
<title xml:lang="en" level="a">Evaluation of affymetrix severe acute respiratory syndrome resequencing GeneChips in characterization of the genomes of two strains of coronavirus infecting humans</title>
<author>
<name sortKey="Sulaiman, Irshad M" sort="Sulaiman, Irshad M" uniqKey="Sulaiman I" first="Irshad M." last="Sulaiman">Irshad M. Sulaiman</name>
<affiliation wicri:level="1">
<inist:fA14 i1="01">
<s1>Biotechnology Core Facility Branch, Scientific Resources Program, National Center for Infectious Diseases, Centers for Disease Control and Prevention</s1>
<s2>Atlanta, Georgia 30333</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
</affiliation>
</author>
<author>
<name sortKey="Xin Liu" sort="Xin Liu" uniqKey="Xin Liu" last="Xin Liu">XIN LIU</name>
<affiliation wicri:level="1">
<inist:fA14 i1="02">
<s1>Respiratory and Enteric Viruses Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention</s1>
<s2>Atlanta, Georgia 30333</s2>
<s3>USA</s3>
<sZ>2 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
</affiliation>
</author>
<author>
<name sortKey="Frace, Michael" sort="Frace, Michael" uniqKey="Frace M" first="Michael" last="Frace">Michael Frace</name>
<affiliation wicri:level="1">
<inist:fA14 i1="01">
<s1>Biotechnology Core Facility Branch, Scientific Resources Program, National Center for Infectious Diseases, Centers for Disease Control and Prevention</s1>
<s2>Atlanta, Georgia 30333</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
</affiliation>
</author>
<author>
<name sortKey="Sulaiman, Nikhat" sort="Sulaiman, Nikhat" uniqKey="Sulaiman N" first="Nikhat" last="Sulaiman">Nikhat Sulaiman</name>
<affiliation wicri:level="1">
<inist:fA14 i1="01">
<s1>Biotechnology Core Facility Branch, Scientific Resources Program, National Center for Infectious Diseases, Centers for Disease Control and Prevention</s1>
<s2>Atlanta, Georgia 30333</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
</affiliation>
</author>
<author>
<name sortKey="Olsen Rasmussen, Melissa" sort="Olsen Rasmussen, Melissa" uniqKey="Olsen Rasmussen M" first="Melissa" last="Olsen-Rasmussen">Melissa Olsen-Rasmussen</name>
<affiliation wicri:level="1">
<inist:fA14 i1="01">
<s1>Biotechnology Core Facility Branch, Scientific Resources Program, National Center for Infectious Diseases, Centers for Disease Control and Prevention</s1>
<s2>Atlanta, Georgia 30333</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
</affiliation>
</author>
<author>
<name sortKey="Neuhaus, Elizabeth" sort="Neuhaus, Elizabeth" uniqKey="Neuhaus E" first="Elizabeth" last="Neuhaus">Elizabeth Neuhaus</name>
<affiliation wicri:level="1">
<inist:fA14 i1="01">
<s1>Biotechnology Core Facility Branch, Scientific Resources Program, National Center for Infectious Diseases, Centers for Disease Control and Prevention</s1>
<s2>Atlanta, Georgia 30333</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
</affiliation>
</author>
<author>
<name sortKey="Rota, Paul A" sort="Rota, Paul A" uniqKey="Rota P" first="Paul A." last="Rota">Paul A. Rota</name>
<affiliation wicri:level="1">
<inist:fA14 i1="02">
<s1>Respiratory and Enteric Viruses Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention</s1>
<s2>Atlanta, Georgia 30333</s2>
<s3>USA</s3>
<sZ>2 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
</affiliation>
</author>
<author>
<name sortKey="Wohlhueter, Robert M" sort="Wohlhueter, Robert M" uniqKey="Wohlhueter R" first="Robert M." last="Wohlhueter">Robert M. Wohlhueter</name>
<affiliation wicri:level="1">
<inist:fA14 i1="01">
<s1>Biotechnology Core Facility Branch, Scientific Resources Program, National Center for Infectious Diseases, Centers for Disease Control and Prevention</s1>
<s2>Atlanta, Georgia 30333</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
</affiliation>
</author>
</analytic>
<series>
<title level="j" type="main">Applied and environmental microbiology : (Print)</title>
<title level="j" type="abbreviated">Appl. environ. microbiol. : (Print)</title>
<idno type="ISSN">0099-2240</idno>
<imprint>
<date when="2006">2006</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt>
<title level="j" type="main">Applied and environmental microbiology : (Print)</title>
<title level="j" type="abbreviated">Appl. environ. microbiol. : (Print)</title>
<idno type="ISSN">0099-2240</idno>
</seriesStmt>
</fileDesc>
<profileDesc>
<textClass>
<keywords scheme="KwdEn" xml:lang="en">
<term>Characterization</term>
<term>Coronavirus</term>
<term>Genome</term>
<term>Human</term>
<term>Severe acute respiratory syndrome</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr">
<term>Caractérisation</term>
<term>Génome</term>
<term>Homme</term>
<term>Coronavirus</term>
<term>Syndrome respiratoire aigu sévère</term>
</keywords>
<keywords scheme="Wicri" type="topic" xml:lang="fr">
<term>Homme</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front>
<div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) was discovered during a recent global outbreak of atypical pneumonia. A number of immunologic and molecular studies of the clinical samples led to the conclusion that a novel coronavirus (SARS-CoV) was associated with the outbreak. Later, a SARS resequencing GeneChip was developed by Aflymetrix to characterize the complete genome of SARS-CoV on a single GeneChip. The present study was carried out to evaluate the performance of SARS resequencing GeneChips. Two human SARS-CoV strains (CDC#200301157 and Urbani) were resequenced by the SARS GeneChips. Five overlapping PCR amplicons were generated for each strain and hybridized with these GeneChips. The successfully hybridized GeneChips generated nucleotide sequences of nearly complete genomes for the two SARS-CoV strains with an average call rate of 94.6%. Multiple alignments of nucleotide sequences obtained from SARS GeneChips and conventional sequencing revealed full concordance. Furthermore, the GeneChip-based analysis revealed no additional polymorphic sites. The results of this study suggest that GeneChip-based genome characterization is fast and reproducible. Thus, SARS resequencing GeneChips may be employed as an alternate tool to obtain genome sequences of SARS-CoV strains pathogenic for humans in order to further understand the transmission dynamics of these viruses.</div>
</front>
</TEI>
<inist>
<standard h6="B">
<pA>
<fA01 i1="01" i2="1">
<s0>0099-2240</s0>
</fA01>
<fA02 i1="01">
<s0>AEMIDF</s0>
</fA02>
<fA03 i2="1">
<s0>Appl. environ. microbiol. : (Print)</s0>
</fA03>
<fA05>
<s2>72</s2>
</fA05>
<fA06>
<s2>1</s2>
</fA06>
<fA08 i1="01" i2="1" l="ENG">
<s1>Evaluation of affymetrix severe acute respiratory syndrome resequencing GeneChips in characterization of the genomes of two strains of coronavirus infecting humans</s1>
</fA08>
<fA11 i1="01" i2="1">
<s1>SULAIMAN (Irshad M.)</s1>
</fA11>
<fA11 i1="02" i2="1">
<s1>XIN LIU</s1>
</fA11>
<fA11 i1="03" i2="1">
<s1>FRACE (Michael)</s1>
</fA11>
<fA11 i1="04" i2="1">
<s1>SULAIMAN (Nikhat)</s1>
</fA11>
<fA11 i1="05" i2="1">
<s1>OLSEN-RASMUSSEN (Melissa)</s1>
</fA11>
<fA11 i1="06" i2="1">
<s1>NEUHAUS (Elizabeth)</s1>
</fA11>
<fA11 i1="07" i2="1">
<s1>ROTA (Paul A.)</s1>
</fA11>
<fA11 i1="08" i2="1">
<s1>WOHLHUETER (Robert M.)</s1>
</fA11>
<fA14 i1="01">
<s1>Biotechnology Core Facility Branch, Scientific Resources Program, National Center for Infectious Diseases, Centers for Disease Control and Prevention</s1>
<s2>Atlanta, Georgia 30333</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
</fA14>
<fA14 i1="02">
<s1>Respiratory and Enteric Viruses Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention</s1>
<s2>Atlanta, Georgia 30333</s2>
<s3>USA</s3>
<sZ>2 aut.</sZ>
<sZ>7 aut.</sZ>
</fA14>
<fA20>
<s1>207-211</s1>
</fA20>
<fA21>
<s1>2006</s1>
</fA21>
<fA23 i1="01">
<s0>ENG</s0>
</fA23>
<fA43 i1="01">
<s1>INIST</s1>
<s2>7195</s2>
<s5>354000133228220250</s5>
</fA43>
<fA44>
<s0>0000</s0>
<s1>© 2006 INIST-CNRS. All rights reserved.</s1>
</fA44>
<fA45>
<s0>15 ref.</s0>
</fA45>
<fA47 i1="01" i2="1">
<s0>06-0260043</s0>
</fA47>
<fA60>
<s1>P</s1>
</fA60>
<fA61>
<s0>A</s0>
</fA61>
<fA64 i1="01" i2="1">
<s0>Applied and environmental microbiology : (Print)</s0>
</fA64>
<fA66 i1="01">
<s0>USA</s0>
</fA66>
<fC01 i1="01" l="ENG">
<s0>Severe acute respiratory syndrome (SARS) was discovered during a recent global outbreak of atypical pneumonia. A number of immunologic and molecular studies of the clinical samples led to the conclusion that a novel coronavirus (SARS-CoV) was associated with the outbreak. Later, a SARS resequencing GeneChip was developed by Aflymetrix to characterize the complete genome of SARS-CoV on a single GeneChip. The present study was carried out to evaluate the performance of SARS resequencing GeneChips. Two human SARS-CoV strains (CDC#200301157 and Urbani) were resequenced by the SARS GeneChips. Five overlapping PCR amplicons were generated for each strain and hybridized with these GeneChips. The successfully hybridized GeneChips generated nucleotide sequences of nearly complete genomes for the two SARS-CoV strains with an average call rate of 94.6%. Multiple alignments of nucleotide sequences obtained from SARS GeneChips and conventional sequencing revealed full concordance. Furthermore, the GeneChip-based analysis revealed no additional polymorphic sites. The results of this study suggest that GeneChip-based genome characterization is fast and reproducible. Thus, SARS resequencing GeneChips may be employed as an alternate tool to obtain genome sequences of SARS-CoV strains pathogenic for humans in order to further understand the transmission dynamics of these viruses.</s0>
</fC01>
<fC02 i1="01" i2="X">
<s0>002A05</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE">
<s0>Caractérisation</s0>
<s5>10</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG">
<s0>Characterization</s0>
<s5>10</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA">
<s0>Caracterización</s0>
<s5>10</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE">
<s0>Génome</s0>
<s5>11</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG">
<s0>Genome</s0>
<s5>11</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA">
<s0>Genoma</s0>
<s5>11</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE">
<s0>Homme</s0>
<s5>12</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG">
<s0>Human</s0>
<s5>12</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA">
<s0>Hombre</s0>
<s5>12</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE">
<s0>Coronavirus</s0>
<s2>NW</s2>
<s5>13</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG">
<s0>Coronavirus</s0>
<s2>NW</s2>
<s5>13</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA">
<s0>Coronavirus</s0>
<s2>NW</s2>
<s5>13</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE">
<s0>Syndrome respiratoire aigu sévère</s0>
<s2>NM</s2>
<s5>19</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG">
<s0>Severe acute respiratory syndrome</s0>
<s2>NM</s2>
<s5>19</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA">
<s0>Síndrome respiratorio agudo severo</s0>
<s2>NM</s2>
<s5>19</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE">
<s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG">
<s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA">
<s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE">
<s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG">
<s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA">
<s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE">
<s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG">
<s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA">
<s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE">
<s0>Virose</s0>
</fC07>
<fC07 i1="04" i2="X" l="ENG">
<s0>Viral disease</s0>
</fC07>
<fC07 i1="04" i2="X" l="SPA">
<s0>Virosis</s0>
</fC07>
<fC07 i1="05" i2="X" l="FRE">
<s0>Infection</s0>
</fC07>
<fC07 i1="05" i2="X" l="ENG">
<s0>Infection</s0>
</fC07>
<fC07 i1="05" i2="X" l="SPA">
<s0>Infección</s0>
</fC07>
<fN21>
<s1>163</s1>
</fN21>
</pA>
</standard>
</inist>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/PascalFrancis/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000492 | SxmlIndent | more

Ou

HfdSelect -h $EXPLOR_AREA/Data/PascalFrancis/Curation/biblio.hfd -nk 000492 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    SrasV1
   |flux=    PascalFrancis
   |étape=   Curation
   |type=    RBID
   |clé=     Pascal:06-0260043
   |texte=   Evaluation of affymetrix severe acute respiratory syndrome resequencing GeneChips in characterization of the genomes of two strains of coronavirus infecting humans
}}

Wicri

This area was generated with Dilib version V0.6.33.
Data generation: Tue Apr 28 14:49:16 2020. Site generation: Sat Mar 27 22:06:49 2021