Experience of using convalescent plasma for severe acute respiratory syndrome among healthcare workers in a Taiwan hospital
Identifieur interne : 000426 ( PascalFrancis/Curation ); précédent : 000425; suivant : 000427Experience of using convalescent plasma for severe acute respiratory syndrome among healthcare workers in a Taiwan hospital
Auteurs : Kuo-Ming Yeh [Taïwan] ; Tzong-Shi Chiueh [Taïwan] ; L. K. Siu [Taïwan] ; Jung-Chung Lin [Taïwan] ; Paul K. S. Chan [Hong Kong] ; Ming-Yieh Peng [Taïwan] ; Hsiang-Lin Wan [Taïwan] ; Jenn-Han Chen [Taïwan] ; Bor-Shen Hu [Taïwan] ; Cherng-Lih Perng [Taïwan] ; Jang-Jih Lu [Taïwan] ; Feng-Yee Chang [Taïwan]Source :
- Journal of antimicrobial chemotherapy : (Print) [ 0305-7453 ] ; 2005.
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Abstract
Objectives: To describe the immunological responses and clinical outcome of coronavirus (SARS) infected healthcare workers (HCW) who had been administered with convalescent plasma as a treatment. Methods: Convalescent plasma (500 mL) was obtained from each of three SARS patients and transfused into the three infected HCW. Donors were blood type O and seronegative for hepatitis B and C, HIV, syphilis and human T-cell lymphotropic virus types I and II (HTLV-I and -II). Serum antibody (IgG) titre was >640. Apharesis was performed with a CS 3000 plus cell separator followed by the forming of the convalescent phase plasma. As part of the routine check with donated plasma, the convalescent plasma was confirmed free of residual SARS-CoV by RT-PCR. Serial serum samples obtained from the recipients of the convalescent plasma were collected to undertake real-time quantitative RT-PCR for SARS-CoV for direct measurement of viral concentration. Specific immunoglobulin IgM and IgG concentrations were titrated using an antigen microarray developed in-house. Results: Viral load dropped from 495 x 103, 76 x 103 or 650 x 103 copies/mL to zero or 1 copy/mL one day after transfusion. Anti-SARS-CoV IgM and IgG also increased in a time-dependent manner following transfusion. All three patients survived. One HCW became pregnant subsequently, delivering 13 months after discharge. Positive anti-SARS-CoV IgG was detected in the newborn. Passive transfer of anti-SARS-CoV antibody from the mother was considered as a possibility. Conclusions: All infected HCW whose condition had progressed severely and who had failed to respond to the available treatment, survived after transfusion with convalescent plasma.
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Experience of using convalescent plasma for severe acute respiratory syndrome among healthcare workers in a Taiwan hospital</title><author><name sortKey="Yeh, Kuo Ming" sort="Yeh, Kuo Ming" uniqKey="Yeh K" first="Kuo-Ming" last="Yeh">Kuo-Ming Yeh</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Division of Infectious Diseases and Tropical Medicine, Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center, No. 325, Cheng-Kung Road, Sec. 2</s1><s2>Neihu, Taipei, 114</s2><s3>TWN</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Taïwan</country></affiliation></author><author><name sortKey="Chiueh, Tzong Shi" sort="Chiueh, Tzong Shi" uniqKey="Chiueh T" first="Tzong-Shi" last="Chiueh">Tzong-Shi Chiueh</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Division of Clinical Pathology, Department of Pathology, Tri-Service General Hospital, National Defense Medical Center</s1><s2>Taipei</s2><s3>TWN</s3><sZ>2 aut.</sZ><sZ>7 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Taïwan</country></affiliation></author><author><name sortKey="Siu, L K" sort="Siu, L K" uniqKey="Siu L" first="L. K." last="Siu">L. K. Siu</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Division of Clinical Research, National Health Research Institutes</s1><s2>Taipei</s2><s3>TWN</s3><sZ>3 aut.</sZ></inist:fA14><country>Taïwan</country></affiliation></author><author><name sortKey="Lin, Jung Chung" sort="Lin, Jung Chung" uniqKey="Lin J" first="Jung-Chung" last="Lin">Jung-Chung Lin</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Division of Infectious Diseases and Tropical Medicine, Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center, No. 325, Cheng-Kung Road, Sec. 2</s1><s2>Neihu, Taipei, 114</s2><s3>TWN</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Taïwan</country></affiliation></author><author><name sortKey="Chan, Paul K S" sort="Chan, Paul K S" uniqKey="Chan P" first="Paul K. S." last="Chan">Paul K. S. Chan</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Department of Microbiology, Faculty of Medicine, The Chinese University of Hong Kong</s1><s3>HKG</s3><sZ>5 aut.</sZ></inist:fA14><country>Hong Kong</country></affiliation></author><author><name sortKey="Peng, Ming Yieh" sort="Peng, Ming Yieh" uniqKey="Peng M" first="Ming-Yieh" last="Peng">Ming-Yieh Peng</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Division of Infectious Diseases and Tropical Medicine, Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center, No. 325, Cheng-Kung Road, Sec. 2</s1><s2>Neihu, Taipei, 114</s2><s3>TWN</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Taïwan</country></affiliation></author><author><name sortKey="Wan, Hsiang Lin" sort="Wan, Hsiang Lin" uniqKey="Wan H" first="Hsiang-Lin" last="Wan">Hsiang-Lin Wan</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Division of Clinical Pathology, Department of Pathology, Tri-Service General Hospital, National Defense Medical Center</s1><s2>Taipei</s2><s3>TWN</s3><sZ>2 aut.</sZ><sZ>7 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Taïwan</country></affiliation></author><author><name sortKey="Chen, Jenn Han" sort="Chen, Jenn Han" uniqKey="Chen J" first="Jenn-Han" last="Chen">Jenn-Han Chen</name><affiliation wicri:level="1"><inist:fA14 i1="05"><s1>Biochip R & D Center, School of Dentistry, Tri-Service General Hospital, National Defense Medical Center</s1><s2>Taipei</s2><s3>TWN</s3><sZ>8 aut.</sZ></inist:fA14><country>Taïwan</country></affiliation></author><author><name sortKey="Hu, Bor Shen" sort="Hu, Bor Shen" uniqKey="Hu B" first="Bor-Shen" last="Hu">Bor-Shen Hu</name><affiliation wicri:level="1"><inist:fA14 i1="06"><s1>Division of Infectious Diseases, Taipei Municipal Hoping Hospital</s1><s2>Taipei</s2><s3>TWN</s3><sZ>9 aut.</sZ></inist:fA14><country>Taïwan</country></affiliation></author><author><name sortKey="Perng, Cherng Lih" sort="Perng, Cherng Lih" uniqKey="Perng C" first="Cherng-Lih" last="Perng">Cherng-Lih Perng</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Division of Clinical Pathology, Department of Pathology, Tri-Service General Hospital, National Defense Medical Center</s1><s2>Taipei</s2><s3>TWN</s3><sZ>2 aut.</sZ><sZ>7 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Taïwan</country></affiliation></author><author><name sortKey="Lu, Jang Jih" sort="Lu, Jang Jih" uniqKey="Lu J" first="Jang-Jih" last="Lu">Jang-Jih Lu</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Division of Clinical Pathology, Department of Pathology, Tri-Service General Hospital, National Defense Medical Center</s1><s2>Taipei</s2><s3>TWN</s3><sZ>2 aut.</sZ><sZ>7 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Taïwan</country></affiliation></author><author><name sortKey="Chang, Feng Yee" sort="Chang, Feng Yee" uniqKey="Chang F" first="Feng-Yee" last="Chang">Feng-Yee Chang</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Division of Infectious Diseases and Tropical Medicine, Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center, No. 325, Cheng-Kung Road, Sec. 2</s1><s2>Neihu, Taipei, 114</s2><s3>TWN</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Taïwan</country></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">06-0039651</idno><date when="2005">2005</date><idno type="stanalyst">PASCAL 06-0039651 INIST</idno><idno type="RBID">Pascal:06-0039651</idno><idno type="wicri:Area/PascalFrancis/Corpus">000564</idno><idno type="wicri:Area/PascalFrancis/Curation">000426</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Experience of using convalescent plasma for severe acute respiratory syndrome among healthcare workers in a Taiwan hospital</title><author><name sortKey="Yeh, Kuo Ming" sort="Yeh, Kuo Ming" uniqKey="Yeh K" first="Kuo-Ming" last="Yeh">Kuo-Ming Yeh</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Division of Infectious Diseases and Tropical Medicine, Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center, No. 325, Cheng-Kung Road, Sec. 2</s1><s2>Neihu, Taipei, 114</s2><s3>TWN</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Taïwan</country></affiliation></author><author><name sortKey="Chiueh, Tzong Shi" sort="Chiueh, Tzong Shi" uniqKey="Chiueh T" first="Tzong-Shi" last="Chiueh">Tzong-Shi Chiueh</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Division of Clinical Pathology, Department of Pathology, Tri-Service General Hospital, National Defense Medical Center</s1><s2>Taipei</s2><s3>TWN</s3><sZ>2 aut.</sZ><sZ>7 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Taïwan</country></affiliation></author><author><name sortKey="Siu, L K" sort="Siu, L K" uniqKey="Siu L" first="L. K." last="Siu">L. K. Siu</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Division of Clinical Research, National Health Research Institutes</s1><s2>Taipei</s2><s3>TWN</s3><sZ>3 aut.</sZ></inist:fA14><country>Taïwan</country></affiliation></author><author><name sortKey="Lin, Jung Chung" sort="Lin, Jung Chung" uniqKey="Lin J" first="Jung-Chung" last="Lin">Jung-Chung Lin</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Division of Infectious Diseases and Tropical Medicine, Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center, No. 325, Cheng-Kung Road, Sec. 2</s1><s2>Neihu, Taipei, 114</s2><s3>TWN</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Taïwan</country></affiliation></author><author><name sortKey="Chan, Paul K S" sort="Chan, Paul K S" uniqKey="Chan P" first="Paul K. S." last="Chan">Paul K. S. Chan</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Department of Microbiology, Faculty of Medicine, The Chinese University of Hong Kong</s1><s3>HKG</s3><sZ>5 aut.</sZ></inist:fA14><country>Hong Kong</country></affiliation></author><author><name sortKey="Peng, Ming Yieh" sort="Peng, Ming Yieh" uniqKey="Peng M" first="Ming-Yieh" last="Peng">Ming-Yieh Peng</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Division of Infectious Diseases and Tropical Medicine, Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center, No. 325, Cheng-Kung Road, Sec. 2</s1><s2>Neihu, Taipei, 114</s2><s3>TWN</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Taïwan</country></affiliation></author><author><name sortKey="Wan, Hsiang Lin" sort="Wan, Hsiang Lin" uniqKey="Wan H" first="Hsiang-Lin" last="Wan">Hsiang-Lin Wan</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Division of Clinical Pathology, Department of Pathology, Tri-Service General Hospital, National Defense Medical Center</s1><s2>Taipei</s2><s3>TWN</s3><sZ>2 aut.</sZ><sZ>7 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Taïwan</country></affiliation></author><author><name sortKey="Chen, Jenn Han" sort="Chen, Jenn Han" uniqKey="Chen J" first="Jenn-Han" last="Chen">Jenn-Han Chen</name><affiliation wicri:level="1"><inist:fA14 i1="05"><s1>Biochip R & D Center, School of Dentistry, Tri-Service General Hospital, National Defense Medical Center</s1><s2>Taipei</s2><s3>TWN</s3><sZ>8 aut.</sZ></inist:fA14><country>Taïwan</country></affiliation></author><author><name sortKey="Hu, Bor Shen" sort="Hu, Bor Shen" uniqKey="Hu B" first="Bor-Shen" last="Hu">Bor-Shen Hu</name><affiliation wicri:level="1"><inist:fA14 i1="06"><s1>Division of Infectious Diseases, Taipei Municipal Hoping Hospital</s1><s2>Taipei</s2><s3>TWN</s3><sZ>9 aut.</sZ></inist:fA14><country>Taïwan</country></affiliation></author><author><name sortKey="Perng, Cherng Lih" sort="Perng, Cherng Lih" uniqKey="Perng C" first="Cherng-Lih" last="Perng">Cherng-Lih Perng</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Division of Clinical Pathology, Department of Pathology, Tri-Service General Hospital, National Defense Medical Center</s1><s2>Taipei</s2><s3>TWN</s3><sZ>2 aut.</sZ><sZ>7 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Taïwan</country></affiliation></author><author><name sortKey="Lu, Jang Jih" sort="Lu, Jang Jih" uniqKey="Lu J" first="Jang-Jih" last="Lu">Jang-Jih Lu</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Division of Clinical Pathology, Department of Pathology, Tri-Service General Hospital, National Defense Medical Center</s1><s2>Taipei</s2><s3>TWN</s3><sZ>2 aut.</sZ><sZ>7 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Taïwan</country></affiliation></author><author><name sortKey="Chang, Feng Yee" sort="Chang, Feng Yee" uniqKey="Chang F" first="Feng-Yee" last="Chang">Feng-Yee Chang</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Division of Infectious Diseases and Tropical Medicine, Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center, No. 325, Cheng-Kung Road, Sec. 2</s1><s2>Neihu, Taipei, 114</s2><s3>TWN</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Taïwan</country></affiliation></author></analytic><series><title level="j" type="main">Journal of antimicrobial chemotherapy : (Print)</title><title level="j" type="abbreviated">J. antimicrob. chemother. : (Print)</title><idno type="ISSN">0305-7453</idno><imprint><date when="2005">2005</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of antimicrobial chemotherapy : (Print)</title><title level="j" type="abbreviated">J. antimicrob. chemother. : (Print)</title><idno type="ISSN">0305-7453</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biological fluid</term><term>Blood plasma</term><term>Coronavirus</term><term>Health care staff</term><term>Health staff</term><term>Hospital</term><term>Severe acute respiratory syndrome</term><term>Taiwan</term><term>Treatment</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Liquide biologique</term><term>Plasma sanguin</term><term>Syndrome respiratoire aigu sévère</term><term>Personnel sanitaire</term><term>Equipe soignante</term><term>Hôpital</term><term>Taiwan</term><term>Coronavirus</term><term>Traitement</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Objectives: To describe the immunological responses and clinical outcome of coronavirus (SARS) infected healthcare workers (HCW) who had been administered with convalescent plasma as a treatment. Methods: Convalescent plasma (500 mL) was obtained from each of three SARS patients and transfused into the three infected HCW. Donors were blood type O and seronegative for hepatitis B and C, HIV, syphilis and human T-cell lymphotropic virus types I and II (HTLV-I and -II). Serum antibody (IgG) titre was >640. Apharesis was performed with a CS 3000 plus cell separator followed by the forming of the convalescent phase plasma. As part of the routine check with donated plasma, the convalescent plasma was confirmed free of residual SARS-CoV by RT-PCR. Serial serum samples obtained from the recipients of the convalescent plasma were collected to undertake real-time quantitative RT-PCR for SARS-CoV for direct measurement of viral concentration. Specific immunoglobulin IgM and IgG concentrations were titrated using an antigen microarray developed in-house. Results: Viral load dropped from 495 x 10<sup>3</sup>, 76 x 10<sup>3</sup> or 650 x 10<sup>3</sup> copies/mL to zero or 1 copy/mL one day after transfusion. Anti-SARS-CoV IgM and IgG also increased in a time-dependent manner following transfusion. All three patients survived. One HCW became pregnant subsequently, delivering 13 months after discharge. Positive anti-SARS-CoV IgG was detected in the newborn. Passive transfer of anti-SARS-CoV antibody from the mother was considered as a possibility. Conclusions: All infected HCW whose condition had progressed severely and who had failed to respond to the available treatment, survived after transfusion with convalescent plasma.</div></front></TEI><inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0305-7453</s0></fA01><fA02 i1="01"><s0>JACHDX</s0></fA02><fA03 i2="1"><s0>J. antimicrob. chemother. : (Print)</s0></fA03><fA05><s2>56</s2></fA05><fA06><s2>5</s2></fA06><fA08 i1="01" i2="1" l="ENG"><s1>Experience of using convalescent plasma for severe acute respiratory syndrome among healthcare workers in a Taiwan hospital</s1></fA08><fA11 i1="01" i2="1"><s1>YEH (Kuo-Ming)</s1></fA11><fA11 i1="02" i2="1"><s1>CHIUEH (Tzong-Shi)</s1></fA11><fA11 i1="03" i2="1"><s1>SIU (L. K.)</s1></fA11><fA11 i1="04" i2="1"><s1>LIN (Jung-Chung)</s1></fA11><fA11 i1="05" i2="1"><s1>CHAN (Paul K. S.)</s1></fA11><fA11 i1="06" i2="1"><s1>PENG (Ming-Yieh)</s1></fA11><fA11 i1="07" i2="1"><s1>WAN (Hsiang-Lin)</s1></fA11><fA11 i1="08" i2="1"><s1>CHEN (Jenn-Han)</s1></fA11><fA11 i1="09" i2="1"><s1>HU (Bor-Shen)</s1></fA11><fA11 i1="10" i2="1"><s1>PERNG (Cherng-Lih)</s1></fA11><fA11 i1="11" i2="1"><s1>LU (Jang-Jih)</s1></fA11><fA11 i1="12" i2="1"><s1>CHANG (Feng-Yee)</s1></fA11><fA14 i1="01"><s1>Division of Infectious Diseases and Tropical Medicine, Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center, No. 325, Cheng-Kung Road, Sec. 2</s1><s2>Neihu, Taipei, 114</s2><s3>TWN</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>12 aut.</sZ></fA14><fA14 i1="02"><s1>Division of Clinical Pathology, Department of Pathology, Tri-Service General Hospital, National Defense Medical Center</s1><s2>Taipei</s2><s3>TWN</s3><sZ>2 aut.</sZ><sZ>7 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></fA14><fA14 i1="03"><s1>Division of Clinical Research, National Health Research Institutes</s1><s2>Taipei</s2><s3>TWN</s3><sZ>3 aut.</sZ></fA14><fA14 i1="04"><s1>Department of Microbiology, Faculty of Medicine, The Chinese University of Hong Kong</s1><s3>HKG</s3><sZ>5 aut.</sZ></fA14><fA14 i1="05"><s1>Biochip R & D Center, School of Dentistry, Tri-Service General Hospital, National Defense Medical Center</s1><s2>Taipei</s2><s3>TWN</s3><sZ>8 aut.</sZ></fA14><fA14 i1="06"><s1>Division of Infectious Diseases, Taipei Municipal Hoping Hospital</s1><s2>Taipei</s2><s3>TWN</s3><sZ>9 aut.</sZ></fA14><fA20><s1>919-922</s1></fA20><fA21><s1>2005</s1></fA21><fA23 i1="01"><s0>ENG</s0></fA23><fA43 i1="01"><s1>INIST</s1><s2>17084</s2><s5>354000135205650200</s5></fA43><fA44><s0>0000</s0><s1>© 2006 INIST-CNRS. All rights reserved.</s1></fA44><fA45><s0>18 ref.</s0></fA45><fA47 i1="01" i2="1"><s0>06-0039651</s0></fA47><fA60><s1>P</s1></fA60><fA61><s0>A</s0></fA61><fA64 i1="01" i2="1"><s0>Journal of antimicrobial chemotherapy : (Print)</s0></fA64><fA66 i1="01"><s0>GBR</s0></fA66><fC01 i1="01" l="ENG"><s0>Objectives: To describe the immunological responses and clinical outcome of coronavirus (SARS) infected healthcare workers (HCW) who had been administered with convalescent plasma as a treatment. Methods: Convalescent plasma (500 mL) was obtained from each of three SARS patients and transfused into the three infected HCW. Donors were blood type O and seronegative for hepatitis B and C, HIV, syphilis and human T-cell lymphotropic virus types I and II (HTLV-I and -II). Serum antibody (IgG) titre was >640. Apharesis was performed with a CS 3000 plus cell separator followed by the forming of the convalescent phase plasma. As part of the routine check with donated plasma, the convalescent plasma was confirmed free of residual SARS-CoV by RT-PCR. Serial serum samples obtained from the recipients of the convalescent plasma were collected to undertake real-time quantitative RT-PCR for SARS-CoV for direct measurement of viral concentration. Specific immunoglobulin IgM and IgG concentrations were titrated using an antigen microarray developed in-house. Results: Viral load dropped from 495 x 10<sup>3</sup>, 76 x 10<sup>3</sup> or 650 x 10<sup>3</sup> copies/mL to zero or 1 copy/mL one day after transfusion. Anti-SARS-CoV IgM and IgG also increased in a time-dependent manner following transfusion. All three patients survived. One HCW became pregnant subsequently, delivering 13 months after discharge. Positive anti-SARS-CoV IgG was detected in the newborn. Passive transfer of anti-SARS-CoV antibody from the mother was considered as a possibility. Conclusions: All infected HCW whose condition had progressed severely and who had failed to respond to the available treatment, survived after transfusion with convalescent plasma.</s0></fC01><fC02 i1="01" i2="X"><s0>002B02S05</s0></fC02><fC03 i1="01" i2="X" l="FRE"><s0>Liquide biologique</s0><s5>01</s5></fC03><fC03 i1="01" i2="X" l="ENG"><s0>Biological fluid</s0><s5>01</s5></fC03><fC03 i1="01" i2="X" l="SPA"><s0>Líquido biológico</s0><s5>01</s5></fC03><fC03 i1="02" i2="X" l="FRE"><s0>Plasma sanguin</s0><s5>02</s5></fC03><fC03 i1="02" i2="X" l="ENG"><s0>Blood plasma</s0><s5>02</s5></fC03><fC03 i1="02" i2="X" l="SPA"><s0>Plasma sanguíneo</s0><s5>02</s5></fC03><fC03 i1="03" i2="X" l="FRE"><s0>Syndrome respiratoire aigu sévère</s0><s2>NM</s2><s5>03</s5></fC03><fC03 i1="03" i2="X" l="ENG"><s0>Severe acute respiratory syndrome</s0><s2>NM</s2><s5>03</s5></fC03><fC03 i1="03" i2="X" l="SPA"><s0>Síndrome respiratorio agudo severo</s0><s2>NM</s2><s5>03</s5></fC03><fC03 i1="04" i2="X" l="FRE"><s0>Personnel sanitaire</s0><s5>04</s5></fC03><fC03 i1="04" i2="X" l="ENG"><s0>Health staff</s0><s5>04</s5></fC03><fC03 i1="04" i2="X" l="SPA"><s0>Personal sanitario</s0><s5>04</s5></fC03><fC03 i1="05" i2="X" l="FRE"><s0>Equipe soignante</s0><s5>05</s5></fC03><fC03 i1="05" i2="X" l="ENG"><s0>Health care staff</s0><s5>05</s5></fC03><fC03 i1="05" i2="X" l="SPA"><s0>Equipo de salud</s0><s5>05</s5></fC03><fC03 i1="06" i2="X" l="FRE"><s0>Hôpital</s0><s5>06</s5></fC03><fC03 i1="06" i2="X" l="ENG"><s0>Hospital</s0><s5>06</s5></fC03><fC03 i1="06" i2="X" l="SPA"><s0>Hospital</s0><s5>06</s5></fC03><fC03 i1="07" i2="X" l="FRE"><s0>Taiwan</s0><s2>NG</s2><s5>07</s5></fC03><fC03 i1="07" i2="X" l="ENG"><s0>Taiwan</s0><s2>NG</s2><s5>07</s5></fC03><fC03 i1="07" i2="X" l="SPA"><s0>Taiwan</s0><s2>NG</s2><s5>07</s5></fC03><fC03 i1="08" i2="X" l="FRE"><s0>Coronavirus</s0><s2>NW</s2><s5>09</s5></fC03><fC03 i1="08" i2="X" l="ENG"><s0>Coronavirus</s0><s2>NW</s2><s5>09</s5></fC03><fC03 i1="08" i2="X" l="SPA"><s0>Coronavirus</s0><s2>NW</s2><s5>09</s5></fC03><fC03 i1="09" i2="X" l="FRE"><s0>Traitement</s0><s5>10</s5></fC03><fC03 i1="09" i2="X" l="ENG"><s0>Treatment</s0><s5>10</s5></fC03><fC03 i1="09" i2="X" l="SPA"><s0>Tratamiento</s0><s5>10</s5></fC03><fC07 i1="01" i2="X" l="FRE"><s0>Virose</s0></fC07><fC07 i1="01" i2="X" l="ENG"><s0>Viral disease</s0></fC07><fC07 i1="01" i2="X" l="SPA"><s0>Virosis</s0></fC07><fC07 i1="02" i2="X" l="FRE"><s0>Infection</s0></fC07><fC07 i1="02" i2="X" l="ENG"><s0>Infection</s0></fC07><fC07 i1="02" i2="X" l="SPA"><s0>Infección</s0></fC07><fC07 i1="03" i2="X" l="FRE"><s0>Asie</s0><s2>NG</s2></fC07><fC07 i1="03" i2="X" l="ENG"><s0>Asia</s0><s2>NG</s2></fC07><fC07 i1="03" i2="X" l="SPA"><s0>Asia</s0><s2>NG</s2></fC07><fC07 i1="04" i2="X" l="FRE"><s0>Coronaviridae</s0><s2>NW</s2></fC07><fC07 i1="04" i2="X" l="ENG"><s0>Coronaviridae</s0><s2>NW</s2></fC07><fC07 i1="04" i2="X" l="SPA"><s0>Coronaviridae</s0><s2>NW</s2></fC07><fC07 i1="05" i2="X" l="FRE"><s0>Nidovirales</s0><s2>NW</s2></fC07><fC07 i1="05" i2="X" l="ENG"><s0>Nidovirales</s0><s2>NW</s2></fC07><fC07 i1="05" i2="X" l="SPA"><s0>Nidovirales</s0><s2>NW</s2></fC07><fC07 i1="06" i2="X" l="FRE"><s0>Virus</s0><s2>NW</s2></fC07><fC07 i1="06" i2="X" l="ENG"><s0>Virus</s0><s2>NW</s2></fC07><fC07 i1="06" i2="X" l="SPA"><s0>Virus</s0><s2>NW</s2></fC07><fC07 i1="07" i2="X" l="FRE"><s0>Appareil respiratoire pathologie</s0><s5>37</s5></fC07><fC07 i1="07" i2="X" l="ENG"><s0>Respiratory disease</s0><s5>37</s5></fC07><fC07 i1="07" i2="X" l="SPA"><s0>Aparato respiratorio patología</s0><s5>37</s5></fC07><fC07 i1="08" i2="X" l="FRE"><s0>Poumon pathologie</s0><s5>38</s5></fC07><fC07 i1="08" i2="X" l="ENG"><s0>Lung disease</s0><s5>38</s5></fC07><fC07 i1="08" i2="X" l="SPA"><s0>Pulmón patología</s0><s5>38</s5></fC07><fN21><s1>016</s1></fN21></pA></standard></inist></record>Pour manipuler ce document sous Unix (Dilib)
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