SrasV1, PascalFrancis, Curation, bibRecord, 000387

SARS coronavirus spike polypeptide DNA vaccine priming with recombinant spike polypeptide from Escherichia coli as booster induces high titer of neutralizing antibody against SARS coronavirus

Identifieur interne : 000387 ( PascalFrancis/Curation ); précédent : 000386; suivant : 000388

SARS coronavirus spike polypeptide DNA vaccine priming with recombinant spike polypeptide from Escherichia coli as booster induces high titer of neutralizing antibody against SARS coronavirus

Auteurs : Patrick C. Y. Woo [Hong Kong] ; Susanna K. P. Lau [Hong Kong] ; Hoi-Wah Tsoi [Hong Kong] ; Zhi-Wei Chen [États-Unis] ; Beatrice H. L. Wong [Hong Kong] ; LINQI ZHANG [États-Unis] ; Jim K. H. Chan [Hong Kong] ; Lei-Po Wong [Hong Kong] ; WEI HE [République populaire de Chine] ; CHI MA [République populaire de Chine] ; Kwok-Hung Chan [Hong Kong] ; David D. Ho [États-Unis] ; Kwok-Yung Yuen [Hong Kong]

Source :

Vaccine [ 0264-410X ] ; 2005.

RBID : Pascal:05-0436229

Descripteurs français

Pascal (Inist)
- Virus syndrome respiratoire aigu sévère, Escherichia coli, Polypeptide, Vaccin génétique, DNA recombinant, Rappel vaccination, Anticorps neutralisant.

English descriptors

KwdEn :
- Booster vaccination, Escherichia coli, Genetic vaccine, Neutralizing antibody, Polypeptide, Recombinant DNA, Severe acute respiratory syndrome virus.

Abstract

Different forms of SARS coronavirus (SARS-CoV) spike protein-based vaccines for generation of neutralizing antibody response against SARS-CoV were compared using a mouse model. High IgG levels were detected in mice immunized with intraperitoneal (i.p.) recombinant spike polypeptide generated by Escherichia coli (S-peptide), mice primed with intramuscular (i.m.) tPA-optimize800 DNA vaccine (tPA-S-DNA) and boosted with i.p. S-peptide, mice primed with i.m. CTLA4HingeSARS800 DNA vaccine (CTLA4-S-DNA) and boosted with i.p. S-peptide, mice primed with oral live-attenuated Salmonella typhimurium (Salmonella-S-DNA-control) and boosted with i.p. S-peptide, mice primed with oral live-attenuated S. typhimurium that contained tPA-optimize800 DNA vaccine (Salmonella-tPA-S-DNA) and boosted with i.p. S-peptide, and mice primed with oral live-attenuated S. typhimurium that contained CTLA4HingeSARS800 DNA vaccine (Salmonella-tPA-S-DNA) and boosted with i.p. S-peptide. No statistical significant difference was observed among the Thl/Th2 index among these six groups of mice with high IgG levels. Sera of all six mice immunized with i.p. S-peptide, i.m. DNA vaccine control and oral Salmonella-S-DNA-control showed no neutralizing antibody against SARS-CoV. Sera of the mice immunized with i.m. tPA-S-DNA, i.m. CTLA4-S-DNA, oral Salmonella-S-DNA-control boosted with i.p. S-peptide, oral Salmonella-tPA-S-DNA, oral Salmonella-tPA-S-DNA boosted with i.p S-peptide, oral Salmonella-CTLA4-S-DNA and oral Salmonella-CTLA4-S-DNA boosted with i.p. S-peptide showed neutralizing antibody titers of <1:20-1:160. Sera of all the mice immunized with i.m. tPA-S-DNA boosted with i.p. S-peptide and i.m. CTLA4-S-DNA boosted with i.p. S-peptide showed neutralizing antibody titers of ≥1:1280. The present observation may have major practical value, such as immunization of civet cats, since production of recombinant proteins from E. coli is far less expensive than production of recombinant proteins using eukaryotic systems.

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Pascal:05-0436229

Le document en format XML

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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">SARS coronavirus spike polypeptide DNA vaccine priming with recombinant spike polypeptide from Escherichia coli as booster induces high titer of neutralizing antibody against SARS coronavirus</title>
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<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, The University of Hong Kong, Room 423, University Pathology Building, Queen Mary Hospital</s1>
<s3>HKG</s3>
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<author><name sortKey="Tsoi, Hoi Wah" sort="Tsoi, Hoi Wah" uniqKey="Tsoi H" first="Hoi-Wah" last="Tsoi">Hoi-Wah Tsoi</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, The University of Hong Kong, Room 423, University Pathology Building, Queen Mary Hospital</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
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<author><name sortKey="Chen, Zhi Wei" sort="Chen, Zhi Wei" uniqKey="Chen Z" first="Zhi-Wei" last="Chen">Zhi-Wei Chen</name>
<affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Aaron Diamond AIDS Research Center</s1>
<s2>NY</s2>
<s3>USA</s3>
<sZ>4 aut.</sZ>
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<author><name sortKey="Wong, Beatrice H L" sort="Wong, Beatrice H L" uniqKey="Wong B" first="Beatrice H. L." last="Wong">Beatrice H. L. Wong</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, The University of Hong Kong, Room 423, University Pathology Building, Queen Mary Hospital</s1>
<s3>HKG</s3>
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<author><name sortKey="Linqi Zhang" sort="Linqi Zhang" uniqKey="Linqi Zhang" last="Linqi Zhang">LINQI ZHANG</name>
<affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Aaron Diamond AIDS Research Center</s1>
<s2>NY</s2>
<s3>USA</s3>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>12 aut.</sZ>
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<author><name sortKey="Chan, Jim K H" sort="Chan, Jim K H" uniqKey="Chan J" first="Jim K. H." last="Chan">Jim K. H. Chan</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, The University of Hong Kong, Room 423, University Pathology Building, Queen Mary Hospital</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
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<country>Hong Kong</country>
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<author><name sortKey="Wong, Lei Po" sort="Wong, Lei Po" uniqKey="Wong L" first="Lei-Po" last="Wong">Lei-Po Wong</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, The University of Hong Kong, Room 423, University Pathology Building, Queen Mary Hospital</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
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<country>Hong Kong</country>
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<author><name sortKey="Wei He" sort="Wei He" uniqKey="Wei He" last="Wei He">WEI HE</name>
<affiliation wicri:level="1"><inist:fA14 i1="05"><s1>Peking Union Medical College</s1>
<s2>Beijing</s2>
<s3>CHN</s3>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
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<country>République populaire de Chine</country>
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</author>
<author><name sortKey="Chi Ma" sort="Chi Ma" uniqKey="Chi Ma" last="Chi Ma">CHI MA</name>
<affiliation wicri:level="1"><inist:fA14 i1="05"><s1>Peking Union Medical College</s1>
<s2>Beijing</s2>
<s3>CHN</s3>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
</affiliation>
</author>
<author><name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok-Hung" last="Chan">Kwok-Hung Chan</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, The University of Hong Kong, Room 423, University Pathology Building, Queen Mary Hospital</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>5 aut.</sZ>
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<sZ>8 aut.</sZ>
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<sZ>13 aut.</sZ>
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<country>Hong Kong</country>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Research Centre of Infection and Immunology, Faculty of Medicine</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>13 aut.</sZ>
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<country>Hong Kong</country>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>State Key Laboratory for Emerging Infectious Diseases, The University of Hong Kong</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>11 aut.</sZ>
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</affiliation>
</author>
<author><name sortKey="Ho, David D" sort="Ho, David D" uniqKey="Ho D" first="David D." last="Ho">David D. Ho</name>
<affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Aaron Diamond AIDS Research Center</s1>
<s2>NY</s2>
<s3>USA</s3>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>12 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
</affiliation>
</author>
<author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, The University of Hong Kong, Room 423, University Pathology Building, Queen Mary Hospital</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>11 aut.</sZ>
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<country>Hong Kong</country>
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<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Research Centre of Infection and Immunology, Faculty of Medicine</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>13 aut.</sZ>
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<country>Hong Kong</country>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>State Key Laboratory for Emerging Infectious Diseases, The University of Hong Kong</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>13 aut.</sZ>
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<country>Hong Kong</country>
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</author>
</analytic>
<series><title level="j" type="main">Vaccine</title>
<title level="j" type="abbreviated">Vaccine</title>
<idno type="ISSN">0264-410X</idno>
<imprint><date when="2005">2005</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Vaccine</title>
<title level="j" type="abbreviated">Vaccine</title>
<idno type="ISSN">0264-410X</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Booster vaccination</term>
<term>Escherichia coli</term>
<term>Genetic vaccine</term>
<term>Neutralizing antibody</term>
<term>Polypeptide</term>
<term>Recombinant DNA</term>
<term>Severe acute respiratory syndrome virus</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Virus syndrome respiratoire aigu sévère</term>
<term>Escherichia coli</term>
<term>Polypeptide</term>
<term>Vaccin génétique</term>
<term>DNA recombinant</term>
<term>Rappel vaccination</term>
<term>Anticorps neutralisant</term>
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<front><div type="abstract" xml:lang="en">Different forms of SARS coronavirus (SARS-CoV) spike protein-based vaccines for generation of neutralizing antibody response against SARS-CoV were compared using a mouse model. High IgG levels were detected in mice immunized with intraperitoneal (i.p.) recombinant spike polypeptide generated by Escherichia coli (S-peptide), mice primed with intramuscular (i.m.) tPA-optimize800 DNA vaccine (tPA-S-DNA) and boosted with i.p. S-peptide, mice primed with i.m. CTLA4HingeSARS800 DNA vaccine (CTLA4-S-DNA) and boosted with i.p. S-peptide, mice primed with oral live-attenuated Salmonella typhimurium (Salmonella-S-DNA-control) and boosted with i.p. S-peptide, mice primed with oral live-attenuated S. typhimurium that contained tPA-optimize800 DNA vaccine (Salmonella-tPA-S-DNA) and boosted with i.p. S-peptide, and mice primed with oral live-attenuated S. typhimurium that contained CTLA4HingeSARS800 DNA vaccine (Salmonella-tPA-S-DNA) and boosted with i.p. S-peptide. No statistical significant difference was observed among the Thl/Th2 index among these six groups of mice with high IgG levels. Sera of all six mice immunized with i.p. S-peptide, i.m. DNA vaccine control and oral Salmonella-S-DNA-control showed no neutralizing antibody against SARS-CoV. Sera of the mice immunized with i.m. tPA-S-DNA, i.m. CTLA4-S-DNA, oral Salmonella-S-DNA-control boosted with i.p. S-peptide, oral Salmonella-tPA-S-DNA, oral Salmonella-tPA-S-DNA boosted with i.p S-peptide, oral Salmonella-CTLA4-S-DNA and oral Salmonella-CTLA4-S-DNA boosted with i.p. S-peptide showed neutralizing antibody titers of <1:20-1:160. Sera of all the mice immunized with i.m. tPA-S-DNA boosted with i.p. S-peptide and i.m. CTLA4-S-DNA boosted with i.p. S-peptide showed neutralizing antibody titers of ≥1:1280. The present observation may have major practical value, such as immunization of civet cats, since production of recombinant proteins from E. coli is far less expensive than production of recombinant proteins using eukaryotic systems.</div>
</front>
</TEI>
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<fA08 i1="01" i2="1" l="ENG"><s1>SARS coronavirus spike polypeptide DNA vaccine priming with recombinant spike polypeptide from Escherichia coli as booster induces high titer of neutralizing antibody against SARS coronavirus</s1>
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<fA11 i1="01" i2="1"><s1>WOO (Patrick C. Y.)</s1>
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<fA11 i1="04" i2="1"><s1>CHEN (Zhi-Wei)</s1>
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<fA11 i1="05" i2="1"><s1>WONG (Beatrice H. L.)</s1>
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<fA11 i1="06" i2="1"><s1>LINQI ZHANG</s1>
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<fA11 i1="07" i2="1"><s1>CHAN (Jim K. H.)</s1>
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<fA11 i1="08" i2="1"><s1>WONG (Lei-Po)</s1>
</fA11>
<fA11 i1="09" i2="1"><s1>WEI HE</s1>
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<fA11 i1="10" i2="1"><s1>CHI MA</s1>
</fA11>
<fA11 i1="11" i2="1"><s1>CHAN (Kwok-Hung)</s1>
</fA11>
<fA11 i1="12" i2="1"><s1>HO (David D.)</s1>
</fA11>
<fA11 i1="13" i2="1"><s1>YUEN (Kwok-Yung)</s1>
</fA11>
<fA14 i1="01"><s1>Department of Microbiology, The University of Hong Kong, Room 423, University Pathology Building, Queen Mary Hospital</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
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<sZ>13 aut.</sZ>
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<fA14 i1="02"><s1>Research Centre of Infection and Immunology, Faculty of Medicine</s1>
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<sZ>11 aut.</sZ>
<sZ>13 aut.</sZ>
</fA14>
<fA14 i1="03"><s1>State Key Laboratory for Emerging Infectious Diseases, The University of Hong Kong</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>13 aut.</sZ>
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<fA14 i1="04"><s1>Aaron Diamond AIDS Research Center</s1>
<s2>NY</s2>
<s3>USA</s3>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>12 aut.</sZ>
</fA14>
<fA14 i1="05"><s1>Peking Union Medical College</s1>
<s2>Beijing</s2>
<s3>CHN</s3>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
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<fA20><s1>4959-4968</s1>
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<fA23 i1="01"><s0>ENG</s0>
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<s2>20289</s2>
<s5>354000131788440010</s5>
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<fA44><s0>0000</s0>
<s1>© 2005 INIST-CNRS. All rights reserved.</s1>
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<fA47 i1="01" i2="1"><s0>05-0436229</s0>
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<fA60><s1>P</s1>
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<fC01 i1="01" l="ENG"><s0>Different forms of SARS coronavirus (SARS-CoV) spike protein-based vaccines for generation of neutralizing antibody response against SARS-CoV were compared using a mouse model. High IgG levels were detected in mice immunized with intraperitoneal (i.p.) recombinant spike polypeptide generated by Escherichia coli (S-peptide), mice primed with intramuscular (i.m.) tPA-optimize800 DNA vaccine (tPA-S-DNA) and boosted with i.p. S-peptide, mice primed with i.m. CTLA4HingeSARS800 DNA vaccine (CTLA4-S-DNA) and boosted with i.p. S-peptide, mice primed with oral live-attenuated Salmonella typhimurium (Salmonella-S-DNA-control) and boosted with i.p. S-peptide, mice primed with oral live-attenuated S. typhimurium that contained tPA-optimize800 DNA vaccine (Salmonella-tPA-S-DNA) and boosted with i.p. S-peptide, and mice primed with oral live-attenuated S. typhimurium that contained CTLA4HingeSARS800 DNA vaccine (Salmonella-tPA-S-DNA) and boosted with i.p. S-peptide. No statistical significant difference was observed among the Thl/Th2 index among these six groups of mice with high IgG levels. Sera of all six mice immunized with i.p. S-peptide, i.m. DNA vaccine control and oral Salmonella-S-DNA-control showed no neutralizing antibody against SARS-CoV. Sera of the mice immunized with i.m. tPA-S-DNA, i.m. CTLA4-S-DNA, oral Salmonella-S-DNA-control boosted with i.p. S-peptide, oral Salmonella-tPA-S-DNA, oral Salmonella-tPA-S-DNA boosted with i.p S-peptide, oral Salmonella-CTLA4-S-DNA and oral Salmonella-CTLA4-S-DNA boosted with i.p. S-peptide showed neutralizing antibody titers of <1:20-1:160. Sera of all the mice immunized with i.m. tPA-S-DNA boosted with i.p. S-peptide and i.m. CTLA4-S-DNA boosted with i.p. S-peptide showed neutralizing antibody titers of ≥1:1280. The present observation may have major practical value, such as immunization of civet cats, since production of recombinant proteins from E. coli is far less expensive than production of recombinant proteins using eukaryotic systems.</s0>
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<fC02 i1="01" i2="X"><s0>002A05F04</s0>
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<s2>NW</s2>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>Severe acute respiratory syndrome virus</s0>
<s2>NW</s2>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Severe acute respiratory syndrome virus</s0>
<s2>NW</s2>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Escherichia coli</s0>
<s2>NS</s2>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Escherichia coli</s0>
<s2>NS</s2>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Escherichia coli</s0>
<s2>NS</s2>
<s5>02</s5>
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<s5>07</s5>
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<s5>07</s5>
</fC03>
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<s5>07</s5>
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<fC03 i1="06" i2="X" l="FRE"><s0>Rappel vaccination</s0>
<s5>08</s5>
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<fC03 i1="06" i2="X" l="ENG"><s0>Booster vaccination</s0>
<s5>08</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Vacunación de refuerzo</s0>
<s5>08</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE"><s0>Anticorps neutralisant</s0>
<s5>09</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG"><s0>Neutralizing antibody</s0>
<s5>09</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA"><s0>anticuerpo neutralizante</s0>
<s5>09</s5>
</fC03>
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<s2>NW</s2>
</fC07>
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<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Coronavirus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Enterobacteriaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Enterobacteriaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Enterobacteriaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Bactérie</s0>
</fC07>
<fC07 i1="06" i2="X" l="ENG"><s0>Bacteria</s0>
</fC07>
<fC07 i1="06" i2="X" l="SPA"><s0>Bacteria</s0>
</fC07>
<fN21><s1>305</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
</standard>
</inist>
</record>

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{{Explor lien
   |wiki=    Sante
   |area=    SrasV1
   |flux=    PascalFrancis
   |étape=   Curation
   |type=    RBID
   |clé=     Pascal:05-0436229
   |texte=   SARS coronavirus spike polypeptide DNA vaccine priming with recombinant spike polypeptide from Escherichia coli as booster induces high titer of neutralizing antibody against SARS coronavirus
}}

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SARS coronavirus spike polypeptide DNA vaccine priming with recombinant spike polypeptide from Escherichia coli as booster induces high titer of neutralizing antibody against SARS coronavirus

SARS coronavirus spike polypeptide DNA vaccine priming with recombinant spike polypeptide from Escherichia coli as booster induces high titer of neutralizing antibody against SARS coronavirus

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