Comprehensive antibody epitope mapping of the nucleocapsid protein of severe acute respiratory syndrome (SARS) coronavirus : Insight into the humoral immunity of SARS
Identifieur interne : 000358 ( PascalFrancis/Curation ); précédent : 000357; suivant : 000359Comprehensive antibody epitope mapping of the nucleocapsid protein of severe acute respiratory syndrome (SARS) coronavirus : Insight into the humoral immunity of SARS
Auteurs : YUNFEI LIANG [République populaire de Chine] ; YING WAN [République populaire de Chine] ; Li-Wen Qiu [République populaire de Chine] ; JINGRAN ZHOU [République populaire de Chine] ; BING NI [République populaire de Chine] ; BO GUO [République populaire de Chine] ; QIANG ZOU [République populaire de Chine] ; LIYUN ZOU [République populaire de Chine] ; WEI ZHOU [République populaire de Chine] ; ZHENGCAI JIA [République populaire de Chine] ; Xiao-Yan Che [République populaire de Chine] ; YUZHANG WU [République populaire de Chine]Source :
- Clinical chemistry : (Baltimore, Md.) [ 0009-9147 ] ; 2005.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Cartographie, Biochimie.
English descriptors
- KwdEn :
Abstract
Background: The epidemic outbreak of severe acute respiratory syndrome (SARS) posed a worldwide threat to public health and economic stability. Although the pandemic has been contained, concerns over its recurrence remain. It is essential to identify specific diagnostic agents and antiviral vaccine candidates to fight this highly contagious disease. Methods: We generated 14 monoclonal antibodies (mAbs) specific to the SARS coronavirus (SARS-CoV) nucleocapsid (N) protein and used these to thoroughly map the N protein antigenic determinants. We identified the immunodominant antigenic sites responsible for the antibodies in sera from SARS patients and antisera from small animals and differentiated the linear from the conformational antibody-combining sites comprising the natural epitopes by use of yeast surface display. Results: We identified 5 conformational and 3 linear epitopes within the entire N protein; 3 conformational and 3 linear epitopes were immunodominant. The antibody responses to the N protein fragments in mammalian sera revealed that 3 regions of the N protein are strong antigenic domains. We expanded the specificity of the N protein epitope and identified 4 novel conformational epitopes (amino acids 1-69, 68-213, 212-341, and 337-422). Conclusion: The antigenic structures identified for the SARS-CoV N protein, the epitope-specific mAbs, and the serum antibody profile in SARS patients have potential use in the clinical diagnosis and understanding of the protective immunity to SARS-CoV.
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Comprehensive antibody epitope mapping of the nucleocapsid protein of severe acute respiratory syndrome (SARS) coronavirus : Insight into the humoral immunity of SARS</title><author><name sortKey="Yunfei Liang" sort="Yunfei Liang" uniqKey="Yunfei Liang" last="Yunfei Liang">YUNFEI LIANG</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>The Institute of Immunology, PLA, The Third Military Medical University</s1><s2>Shapingba District, Chongqing</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>République populaire de Chine</country></affiliation></author><author><name sortKey="Ying Wan" sort="Ying Wan" uniqKey="Ying Wan" last="Ying Wan">YING WAN</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>The Institute of Immunology, PLA, The Third Military Medical 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aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>République populaire de Chine</country></affiliation></author><author><name sortKey="Bing Ni" sort="Bing Ni" uniqKey="Bing Ni" last="Bing Ni">BING NI</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>The Institute of Immunology, PLA, The Third Military Medical University</s1><s2>Shapingba District, Chongqing</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>République populaire de Chine</country></affiliation></author><author><name sortKey="Bo Guo" sort="Bo Guo" uniqKey="Bo Guo" last="Bo Guo">BO GUO</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>The Institute of Immunology, PLA, The Third Military Medical University</s1><s2>Shapingba District, Chongqing</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>République populaire de Chine</country></affiliation></author><author><name sortKey="Qiang Zou" sort="Qiang Zou" uniqKey="Qiang Zou" last="Qiang Zou">QIANG ZOU</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>The Institute of Immunology, PLA, The Third Military Medical University</s1><s2>Shapingba District, Chongqing</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>République populaire de Chine</country></affiliation></author><author><name sortKey="Liyun Zou" sort="Liyun Zou" uniqKey="Liyun Zou" last="Liyun Zou">LIYUN ZOU</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>The Institute of Immunology, PLA, The Third Military Medical University</s1><s2>Shapingba District, Chongqing</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>République populaire de Chine</country></affiliation></author><author><name sortKey="Wei Zhou" sort="Wei Zhou" uniqKey="Wei Zhou" last="Wei Zhou">WEI ZHOU</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>The Institute of Immunology, PLA, The Third Military Medical University</s1><s2>Shapingba District, Chongqing</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>République populaire de Chine</country></affiliation></author><author><name sortKey="Zhengcai Jia" sort="Zhengcai Jia" uniqKey="Zhengcai Jia" last="Zhengcai Jia">ZHENGCAI JIA</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>The Institute of Immunology, PLA, The Third Military Medical University</s1><s2>Shapingba District, Chongqing</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>République populaire de Chine</country></affiliation></author><author><name sortKey="Che, Xiao Yan" sort="Che, Xiao Yan" uniqKey="Che X" first="Xiao-Yan" last="Che">Xiao-Yan Che</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Central Laboratory, Zhujiang Hospital, The Southern Medical University</s1><s2>Guangzhou</s2><s3>CHN</s3><sZ>3 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>République populaire de Chine</country></affiliation></author><author><name sortKey="Yuzhang Wu" sort="Yuzhang Wu" uniqKey="Yuzhang Wu" last="Yuzhang Wu">YUZHANG WU</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>The Institute of Immunology, PLA, The Third Military Medical University</s1><s2>Shapingba District, Chongqing</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>République populaire de Chine</country></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">05-0347652</idno><date when="2005">2005</date><idno type="stanalyst">PASCAL 05-0347652 INIST</idno><idno type="RBID">Pascal:05-0347652</idno><idno type="wicri:Area/PascalFrancis/Corpus">000632</idno><idno type="wicri:Area/PascalFrancis/Curation">000358</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Comprehensive antibody epitope mapping of the nucleocapsid protein of severe acute respiratory syndrome (SARS) coronavirus : Insight into the humoral immunity of SARS</title><author><name sortKey="Yunfei Liang" sort="Yunfei Liang" uniqKey="Yunfei Liang" last="Yunfei Liang">YUNFEI LIANG</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>The Institute of Immunology, PLA, The Third Military Medical University</s1><s2>Shapingba District, Chongqing</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>République populaire de Chine</country></affiliation></author><author><name sortKey="Ying Wan" sort="Ying Wan" uniqKey="Ying Wan" last="Ying Wan">YING WAN</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>The Institute of Immunology, PLA, The Third Military Medical University</s1><s2>Shapingba District, Chongqing</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>République populaire de Chine</country></affiliation></author><author><name sortKey="Qiu, Li Wen" sort="Qiu, Li Wen" uniqKey="Qiu L" first="Li-Wen" last="Qiu">Li-Wen Qiu</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Central Laboratory, Zhujiang Hospital, The Southern Medical University</s1><s2>Guangzhou</s2><s3>CHN</s3><sZ>3 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>République populaire de Chine</country></affiliation></author><author><name sortKey="Jingran Zhou" sort="Jingran Zhou" uniqKey="Jingran Zhou" last="Jingran Zhou">JINGRAN ZHOU</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>The Institute of Immunology, PLA, The Third Military Medical University</s1><s2>Shapingba District, Chongqing</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>République populaire de Chine</country></affiliation></author><author><name sortKey="Bing Ni" sort="Bing Ni" uniqKey="Bing Ni" last="Bing Ni">BING NI</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>The Institute of Immunology, PLA, The Third Military Medical University</s1><s2>Shapingba District, Chongqing</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>République populaire de Chine</country></affiliation></author><author><name sortKey="Bo Guo" sort="Bo Guo" uniqKey="Bo Guo" last="Bo Guo">BO GUO</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>The Institute of Immunology, PLA, The Third Military Medical University</s1><s2>Shapingba District, Chongqing</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>République populaire de Chine</country></affiliation></author><author><name sortKey="Qiang Zou" sort="Qiang Zou" uniqKey="Qiang Zou" last="Qiang Zou">QIANG ZOU</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>The Institute of Immunology, PLA, The Third Military Medical University</s1><s2>Shapingba District, Chongqing</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>République populaire de Chine</country></affiliation></author><author><name sortKey="Liyun Zou" sort="Liyun Zou" uniqKey="Liyun Zou" last="Liyun Zou">LIYUN ZOU</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>The Institute of Immunology, PLA, The Third Military Medical University</s1><s2>Shapingba District, Chongqing</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>République populaire de Chine</country></affiliation></author><author><name sortKey="Wei Zhou" sort="Wei Zhou" uniqKey="Wei Zhou" last="Wei Zhou">WEI ZHOU</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>The Institute of Immunology, PLA, The Third Military Medical University</s1><s2>Shapingba District, Chongqing</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>République populaire de Chine</country></affiliation></author><author><name sortKey="Zhengcai Jia" sort="Zhengcai Jia" uniqKey="Zhengcai Jia" last="Zhengcai Jia">ZHENGCAI JIA</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>The Institute of Immunology, PLA, The Third Military Medical University</s1><s2>Shapingba District, Chongqing</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>République populaire de Chine</country></affiliation></author><author><name sortKey="Che, Xiao Yan" sort="Che, Xiao Yan" uniqKey="Che X" first="Xiao-Yan" last="Che">Xiao-Yan Che</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Central Laboratory, Zhujiang Hospital, The Southern Medical University</s1><s2>Guangzhou</s2><s3>CHN</s3><sZ>3 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>République populaire de Chine</country></affiliation></author><author><name sortKey="Yuzhang Wu" sort="Yuzhang Wu" uniqKey="Yuzhang Wu" last="Yuzhang Wu">YUZHANG WU</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>The Institute of Immunology, PLA, The Third Military Medical University</s1><s2>Shapingba District, Chongqing</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>République populaire de Chine</country></affiliation></author></analytic><series><title level="j" type="main">Clinical chemistry : (Baltimore, Md.)</title><title level="j" type="abbreviated">Clin. chem. : (Baltim. Md.)</title><idno type="ISSN">0009-9147</idno><imprint><date when="2005">2005</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Clinical chemistry : (Baltimore, Md.)</title><title level="j" type="abbreviated">Clin. chem. : (Baltim. Md.)</title><idno type="ISSN">0009-9147</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibody</term><term>Antigenic determinant</term><term>Biochemistry</term><term>Cartography</term><term>Clinical biology</term><term>Coronavirus</term><term>Humoral immunity</term><term>Molecular biology</term><term>Nucleocapsid</term><term>Protein</term><term>Severe acute respiratory syndrome</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Anticorps</term><term>Déterminant antigénique</term><term>Cartographie</term><term>Nucléocapside</term><term>Protéine</term><term>Syndrome respiratoire aigu sévère</term><term>Coronavirus</term><term>Immunité humorale</term><term>Biochimie</term><term>Biologie clinique</term><term>Biologie moléculaire</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Cartographie</term><term>Biochimie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Background: The epidemic outbreak of severe acute respiratory syndrome (SARS) posed a worldwide threat to public health and economic stability. Although the pandemic has been contained, concerns over its recurrence remain. It is essential to identify specific diagnostic agents and antiviral vaccine candidates to fight this highly contagious disease. Methods: We generated 14 monoclonal antibodies (mAbs) specific to the SARS coronavirus (SARS-CoV) nucleocapsid (N) protein and used these to thoroughly map the N protein antigenic determinants. We identified the immunodominant antigenic sites responsible for the antibodies in sera from SARS patients and antisera from small animals and differentiated the linear from the conformational antibody-combining sites comprising the natural epitopes by use of yeast surface display. Results: We identified 5 conformational and 3 linear epitopes within the entire N protein; 3 conformational and 3 linear epitopes were immunodominant. The antibody responses to the N protein fragments in mammalian sera revealed that 3 regions of the N protein are strong antigenic domains. We expanded the specificity of the N protein epitope and identified 4 novel conformational epitopes (amino acids 1-69, 68-213, 212-341, and 337-422). Conclusion: The antigenic structures identified for the SARS-CoV N protein, the epitope-specific mAbs, and the serum antibody profile in SARS patients have potential use in the clinical diagnosis and understanding of the protective immunity to SARS-CoV.</div></front></TEI><inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0009-9147</s0></fA01><fA02 i1="01"><s0>CLCHAU</s0></fA02><fA03 i2="1"><s0>Clin. chem. : (Baltim. Md.)</s0></fA03><fA05><s2>51</s2></fA05><fA06><s2>8</s2></fA06><fA08 i1="01" i2="1" l="ENG"><s1>Comprehensive antibody epitope mapping of the nucleocapsid protein of severe acute respiratory syndrome (SARS) coronavirus : Insight into the humoral immunity of SARS</s1></fA08><fA11 i1="01" i2="1"><s1>YUNFEI LIANG</s1></fA11><fA11 i1="02" i2="1"><s1>YING WAN</s1></fA11><fA11 i1="03" i2="1"><s1>QIU (Li-Wen)</s1></fA11><fA11 i1="04" i2="1"><s1>JINGRAN ZHOU</s1></fA11><fA11 i1="05" i2="1"><s1>BING NI</s1></fA11><fA11 i1="06" i2="1"><s1>BO GUO</s1></fA11><fA11 i1="07" i2="1"><s1>QIANG ZOU</s1></fA11><fA11 i1="08" i2="1"><s1>LIYUN ZOU</s1></fA11><fA11 i1="09" i2="1"><s1>WEI ZHOU</s1></fA11><fA11 i1="10" i2="1"><s1>ZHENGCAI JIA</s1></fA11><fA11 i1="11" i2="1"><s1>CHE (Xiao-Yan)</s1></fA11><fA11 i1="12" i2="1"><s1>YUZHANG WU</s1></fA11><fA14 i1="01"><s1>The Institute of Immunology, PLA, The Third Military Medical University</s1><s2>Shapingba District, Chongqing</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>12 aut.</sZ></fA14><fA14 i1="02"><s1>Central Laboratory, Zhujiang Hospital, The Southern Medical University</s1><s2>Guangzhou</s2><s3>CHN</s3><sZ>3 aut.</sZ><sZ>11 aut.</sZ></fA14><fA20><s1>1382-1396</s1></fA20><fA21><s1>2005</s1></fA21><fA23 i1="01"><s0>ENG</s0></fA23><fA43 i1="01"><s1>INIST</s1><s2>7603</s2><s5>354000138358560100</s5></fA43><fA44><s0>0000</s0><s1>© 2005 INIST-CNRS. All rights reserved.</s1></fA44><fA45><s0>53 ref.</s0></fA45><fA47 i1="01" i2="1"><s0>05-0347652</s0></fA47><fA60><s1>P</s1></fA60><fA61><s0>A</s0></fA61><fA64 i1="01" i2="1"><s0>Clinical chemistry : (Baltimore, Md.)</s0></fA64><fA66 i1="01"><s0>USA</s0></fA66><fC01 i1="01" l="ENG"><s0>Background: The epidemic outbreak of severe acute respiratory syndrome (SARS) posed a worldwide threat to public health and economic stability. Although the pandemic has been contained, concerns over its recurrence remain. It is essential to identify specific diagnostic agents and antiviral vaccine candidates to fight this highly contagious disease. Methods: We generated 14 monoclonal antibodies (mAbs) specific to the SARS coronavirus (SARS-CoV) nucleocapsid (N) protein and used these to thoroughly map the N protein antigenic determinants. We identified the immunodominant antigenic sites responsible for the antibodies in sera from SARS patients and antisera from small animals and differentiated the linear from the conformational antibody-combining sites comprising the natural epitopes by use of yeast surface display. Results: We identified 5 conformational and 3 linear epitopes within the entire N protein; 3 conformational and 3 linear epitopes were immunodominant. The antibody responses to the N protein fragments in mammalian sera revealed that 3 regions of the N protein are strong antigenic domains. We expanded the specificity of the N protein epitope and identified 4 novel conformational epitopes (amino acids 1-69, 68-213, 212-341, and 337-422). Conclusion: The antigenic structures identified for the SARS-CoV N protein, the epitope-specific mAbs, and the serum antibody profile in SARS patients have potential use in the clinical diagnosis and understanding of the protective immunity to SARS-CoV.</s0></fC01><fC02 i1="01" i2="X"><s0>002B24</s0></fC02><fC02 i1="02" i2="X"><s0>002A02</s0></fC02><fC03 i1="01" i2="X" l="FRE"><s0>Anticorps</s0><s5>02</s5></fC03><fC03 i1="01" i2="X" l="ENG"><s0>Antibody</s0><s5>02</s5></fC03><fC03 i1="01" i2="X" l="SPA"><s0>Anticuerpo</s0><s5>02</s5></fC03><fC03 i1="02" i2="X" l="FRE"><s0>Déterminant antigénique</s0><s5>03</s5></fC03><fC03 i1="02" i2="X" l="ENG"><s0>Antigenic determinant</s0><s5>03</s5></fC03><fC03 i1="02" i2="X" l="SPA"><s0>Determinante antigénico</s0><s5>03</s5></fC03><fC03 i1="03" i2="X" l="FRE"><s0>Cartographie</s0><s5>05</s5></fC03><fC03 i1="03" i2="X" l="ENG"><s0>Cartography</s0><s5>05</s5></fC03><fC03 i1="03" i2="X" l="SPA"><s0>Cartografía</s0><s5>05</s5></fC03><fC03 i1="04" i2="X" l="FRE"><s0>Nucléocapside</s0><s5>06</s5></fC03><fC03 i1="04" i2="X" l="ENG"><s0>Nucleocapsid</s0><s5>06</s5></fC03><fC03 i1="04" i2="X" l="SPA"><s0>Nucleocápside</s0><s5>06</s5></fC03><fC03 i1="05" i2="X" l="FRE"><s0>Protéine</s0><s5>08</s5></fC03><fC03 i1="05" i2="X" l="ENG"><s0>Protein</s0><s5>08</s5></fC03><fC03 i1="05" i2="X" l="SPA"><s0>Proteína</s0><s5>08</s5></fC03><fC03 i1="06" i2="X" l="FRE"><s0>Syndrome respiratoire aigu sévère</s0><s2>NM</s2><s5>09</s5></fC03><fC03 i1="06" i2="X" l="ENG"><s0>Severe acute respiratory syndrome</s0><s2>NM</s2><s5>09</s5></fC03><fC03 i1="06" i2="X" l="SPA"><s0>Síndrome respiratorio agudo severo</s0><s2>NM</s2><s5>09</s5></fC03><fC03 i1="07" i2="X" l="FRE"><s0>Coronavirus</s0><s2>NW</s2><s5>11</s5></fC03><fC03 i1="07" i2="X" l="ENG"><s0>Coronavirus</s0><s2>NW</s2><s5>11</s5></fC03><fC03 i1="07" i2="X" l="SPA"><s0>Coronavirus</s0><s2>NW</s2><s5>11</s5></fC03><fC03 i1="08" i2="X" l="FRE"><s0>Immunité humorale</s0><s5>12</s5></fC03><fC03 i1="08" i2="X" l="ENG"><s0>Humoral immunity</s0><s5>12</s5></fC03><fC03 i1="08" i2="X" l="SPA"><s0>Inmunidad humoral</s0><s5>12</s5></fC03><fC03 i1="09" i2="X" l="FRE"><s0>Biochimie</s0><s5>14</s5></fC03><fC03 i1="09" i2="X" l="ENG"><s0>Biochemistry</s0><s5>14</s5></fC03><fC03 i1="09" i2="X" l="SPA"><s0>Bioquímica</s0><s5>14</s5></fC03><fC03 i1="10" i2="X" l="FRE"><s0>Biologie clinique</s0><s5>15</s5></fC03><fC03 i1="10" i2="X" l="ENG"><s0>Clinical biology</s0><s5>15</s5></fC03><fC03 i1="10" i2="X" l="SPA"><s0>Biología clínica</s0><s5>15</s5></fC03><fC03 i1="11" i2="X" l="FRE"><s0>Biologie moléculaire</s0><s5>17</s5></fC03><fC03 i1="11" i2="X" l="ENG"><s0>Molecular biology</s0><s5>17</s5></fC03><fC03 i1="11" i2="X" l="SPA"><s0>Biología molecular</s0><s5>17</s5></fC03><fC07 i1="01" i2="X" l="FRE"><s0>Virose</s0></fC07><fC07 i1="01" i2="X" l="ENG"><s0>Viral disease</s0></fC07><fC07 i1="01" i2="X" l="SPA"><s0>Virosis</s0></fC07><fC07 i1="02" i2="X" l="FRE"><s0>Infection</s0></fC07><fC07 i1="02" i2="X" l="ENG"><s0>Infection</s0></fC07><fC07 i1="02" i2="X" l="SPA"><s0>Infección</s0></fC07><fC07 i1="03" i2="X" l="FRE"><s0>Coronaviridae</s0><s2>NW</s2></fC07><fC07 i1="03" i2="X" l="ENG"><s0>Coronaviridae</s0><s2>NW</s2></fC07><fC07 i1="03" i2="X" l="SPA"><s0>Coronaviridae</s0><s2>NW</s2></fC07><fC07 i1="04" i2="X" l="FRE"><s0>Nidovirales</s0><s2>NW</s2></fC07><fC07 i1="04" i2="X" l="ENG"><s0>Nidovirales</s0><s2>NW</s2></fC07><fC07 i1="04" i2="X" l="SPA"><s0>Nidovirales</s0><s2>NW</s2></fC07><fC07 i1="05" i2="X" l="FRE"><s0>Virus</s0><s2>NW</s2></fC07><fC07 i1="05" i2="X" l="ENG"><s0>Virus</s0><s2>NW</s2></fC07><fC07 i1="05" i2="X" l="SPA"><s0>Virus</s0><s2>NW</s2></fC07><fC07 i1="06" i2="X" l="FRE"><s0>Appareil respiratoire pathologie</s0><s5>37</s5></fC07><fC07 i1="06" i2="X" l="ENG"><s0>Respiratory disease</s0><s5>37</s5></fC07><fC07 i1="06" i2="X" l="SPA"><s0>Aparato respiratorio patología</s0><s5>37</s5></fC07><fC07 i1="07" i2="X" l="FRE"><s0>Poumon pathologie</s0><s5>38</s5></fC07><fC07 i1="07" i2="X" l="ENG"><s0>Lung disease</s0><s5>38</s5></fC07><fC07 i1="07" i2="X" l="SPA"><s0>Pulmón patología</s0><s5>38</s5></fC07><fN21><s1>241</s1></fN21><fN44 i1="01"><s1>OTO</s1></fN44><fN82><s1>OTO</s1></fN82></pA></standard></inist></record>Pour manipuler ce document sous Unix (Dilib)
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