SrasV1, PascalFrancis, Curation, bibRecord, 000305

Comparative host gene transcription by microarray analysis early after infection of the Huh7 cell line by severe acute respiratory syndrome coronavirus and human coronavirus 229E

Identifieur interne : 000305 ( PascalFrancis/Curation ); précédent : 000304; suivant : 000306

Comparative host gene transcription by microarray analysis early after infection of the Huh7 cell line by severe acute respiratory syndrome coronavirus and human coronavirus 229E

Auteurs : Bone S. F. Tang [Hong Kong] ; Kwok-Hung Chan [Hong Kong] ; Vincent C. C. Cheng [Hong Kong] ; Patrick C. Y. Woo [Hong Kong] ; Susanna K. P. Lau [Hong Kong] ; Clarence C. K. Lam [Hong Kong] ; Tsun-Leung Chan [Hong Kong] ; Alan K. L. Wu [Hong Kong] ; Ivan F. N. Hung [Hong Kong] ; Suet-Yi Leung [Hong Kong] ; Kwok-Yung Yuen [Hong Kong]

Source :

Journal of virology [ 0022-538X ] ; 2005.

RBID : Pascal:05-0231505

Descripteurs français

Pascal (Inist)
- Coronavirus, Homme, Gène, Transcription, Lignée cellulaire, Microbiologie, Virologie, Syndrome respiratoire aigu sévère.
Wicri :
- topic : Homme.

English descriptors

KwdEn :
- Cell line, Coronavirus, Gene, Human, Microbiology, Severe acute respiratory syndrome, Transcription, Virology.

Abstract

The pathogenesis of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) at the cellular level is unclear. No human cell line was previously known to be susceptible to both SARS-CoV and other human coronaviruses. Huh7 cells were found to be susceptible to both SARS-CoV, associated with SARS, and human coronavirus 229E (HCoV-229E), usually associated with the common cold. Highly lytic and productive rates of infections within 48 h of inoculation were reproducible with both viruses. The early transcriptional profiles of host cell response to both types of infection at 2 and 4 h postinoculation were determined by using the Affymetrix HG-U133A microarray (about 22,000 genes). Much more perturbation of cellular gene transcription was observed after infection by SARS-CoV than after infection by HCoV-229E. Besides the upregulation of genes associated with apoptosis, which was exactly opposite to the previously reported effect of SARS-CoV in a colonic carcinoma cell line, genes related to inflammation, stress response, and procoagulation were also upregulated. These findings were confirmed by semiquantitative reverse transcription-PCR, reverse transcription-quantitative PCR for mRNA of genes, and immunoassays for some encoded proteins. These transcriptomal changes are compatible with the histological changes of pulmonary vasculitis and microvascular thrombosis in addition to the diffuse alveolar damage involving the pneumocytes.

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A03		`1`		`@0 J. virol.`
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A08	`01`	`1`	`ENG`	`@1 Comparative host gene transcription by microarray analysis early after infection of the Huh7 cell line by severe acute respiratory syndrome coronavirus and human coronavirus 229E`
A11	`01`	`1`		`@1 TANG (Bone S. F.)`
A11	`02`	`1`		`@1 CHAN (Kwok-Hung)`
A11	`03`	`1`		`@1 CHENG (Vincent C. C.)`
A11	`04`	`1`		`@1 WOO (Patrick C. Y.)`
A11	`05`	`1`		`@1 LAU (Susanna K. P.)`
A11	`06`	`1`		`@1 LAM (Clarence C. K.)`
A11	`07`	`1`		`@1 CHAN (Tsun-Leung)`
A11	`08`	`1`		`@1 WU (Alan K. L.)`
A11	`09`	`1`		`@1 HUNG (Ivan F. N.)`
A11	`10`	`1`		`@1 LEUNG (Suet-Yi)`
A11	`11`	`1`		`@1 YUEN (Kwok-Yung)`
A14	`01`			`@1 Department of Microbiology, Centre of Infection and Immunology, State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong @3 HKG @Z 1 aut. @Z 2 aut. @Z 3 aut. @Z 4 aut. @Z 5 aut. @Z 8 aut. @Z 9 aut. @Z 11 aut.`
A14	`02`			`@1 Division of Haematology, Department of Pathology, The University of Hong Kong @3 HKG @Z 6 aut.`
A14	`03`			`@1 Division of Anatomical Pathology, Department of Pathology, The University of Hong Kong @3 HKG @Z 7 aut. @Z 10 aut.`
A20				`@1 6180-6193`
A21				`@1 2005`
A23	`01`			`@0 ENG`
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A44				`@0 0000 @1 © 2005 INIST-CNRS. All rights reserved.`
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A64	`01`	`1`		`@0 Journal of virology`
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C01	`01`		`ENG`	@0 The pathogenesis of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) at the cellular level is unclear. No human cell line was previously known to be susceptible to both SARS-CoV and other human coronaviruses. Huh7 cells were found to be susceptible to both SARS-CoV, associated with SARS, and human coronavirus 229E (HCoV-229E), usually associated with the common cold. Highly lytic and productive rates of infections within 48 h of inoculation were reproducible with both viruses. The early transcriptional profiles of host cell response to both types of infection at 2 and 4 h postinoculation were determined by using the Affymetrix HG-U133A microarray (about 22,000 genes). Much more perturbation of cellular gene transcription was observed after infection by SARS-CoV than after infection by HCoV-229E. Besides the upregulation of genes associated with apoptosis, which was exactly opposite to the previously reported effect of SARS-CoV in a colonic carcinoma cell line, genes related to inflammation, stress response, and procoagulation were also upregulated. These findings were confirmed by semiquantitative reverse transcription-PCR, reverse transcription-quantitative PCR for mRNA of genes, and immunoassays for some encoded proteins. These transcriptomal changes are compatible with the histological changes of pulmonary vasculitis and microvascular thrombosis in addition to the diffuse alveolar damage involving the pneumocytes.
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C03	`05`	`X`	`FRE`	`@0 Lignée cellulaire @5 07`
C03	`05`	`X`	`ENG`	`@0 Cell line @5 07`
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C03	`08`	`X`	`FRE`	`@0 Syndrome respiratoire aigu sévère @2 NM @5 14`
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C03	`08`	`X`	`SPA`	`@0 Síndrome respiratorio agudo severo @2 NM @5 14`
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C07	`07`	`X`	`FRE`	`@0 Poumon pathologie @5 16`
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N44	`01`			`@1 OTO`
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Le document en format XML

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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Comparative host gene transcription by microarray analysis early after infection of the Huh7 cell line by severe acute respiratory syndrome coronavirus and human coronavirus 229E</title>
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<author><name sortKey="Chan, Tsun Leung" sort="Chan, Tsun Leung" uniqKey="Chan T" first="Tsun-Leung" last="Chan">Tsun-Leung Chan</name>
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<author><name sortKey="Wu, Alan K L" sort="Wu, Alan K L" uniqKey="Wu A" first="Alan K. L." last="Wu">Alan K. L. Wu</name>
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<author><name sortKey="Hung, Ivan F N" sort="Hung, Ivan F N" uniqKey="Hung I" first="Ivan F. N." last="Hung">Ivan F. N. Hung</name>
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<author><name sortKey="Leung, Suet Yi" sort="Leung, Suet Yi" uniqKey="Leung S" first="Suet-Yi" last="Leung">Suet-Yi Leung</name>
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<country>Hong Kong</country>
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<author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, Centre of Infection and Immunology, State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong</s1>
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<country>Hong Kong</country>
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<series><title level="j" type="main">Journal of virology</title>
<title level="j" type="abbreviated">J. virol.</title>
<idno type="ISSN">0022-538X</idno>
<imprint><date when="2005">2005</date>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell line</term>
<term>Coronavirus</term>
<term>Gene</term>
<term>Human</term>
<term>Microbiology</term>
<term>Severe acute respiratory syndrome</term>
<term>Transcription</term>
<term>Virology</term>
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<front><div type="abstract" xml:lang="en">The pathogenesis of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) at the cellular level is unclear. No human cell line was previously known to be susceptible to both SARS-CoV and other human coronaviruses. Huh7 cells were found to be susceptible to both SARS-CoV, associated with SARS, and human coronavirus 229E (HCoV-229E), usually associated with the common cold. Highly lytic and productive rates of infections within 48 h of inoculation were reproducible with both viruses. The early transcriptional profiles of host cell response to both types of infection at 2 and 4 h postinoculation were determined by using the Affymetrix HG-U133A microarray (about 22,000 genes). Much more perturbation of cellular gene transcription was observed after infection by SARS-CoV than after infection by HCoV-229E. Besides the upregulation of genes associated with apoptosis, which was exactly opposite to the previously reported effect of SARS-CoV in a colonic carcinoma cell line, genes related to inflammation, stress response, and procoagulation were also upregulated. These findings were confirmed by semiquantitative reverse transcription-PCR, reverse transcription-quantitative PCR for mRNA of genes, and immunoassays for some encoded proteins. These transcriptomal changes are compatible with the histological changes of pulmonary vasculitis and microvascular thrombosis in addition to the diffuse alveolar damage involving the pneumocytes.</div>
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<fA11 i1="07" i2="1"><s1>CHAN (Tsun-Leung)</s1>
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<fA11 i1="08" i2="1"><s1>WU (Alan K. L.)</s1>
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<fA14 i1="01"><s1>Department of Microbiology, Centre of Infection and Immunology, State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong</s1>
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<fA14 i1="02"><s1>Division of Haematology, Department of Pathology, The University of Hong Kong</s1>
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Comparative host gene transcription by microarray analysis early after infection of the Huh7 cell line by severe acute respiratory syndrome coronavirus and human coronavirus 229E

Comparative host gene transcription by microarray analysis early after infection of the Huh7 cell line by severe acute respiratory syndrome coronavirus and human coronavirus 229E

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