SrasV1, PascalFrancis, Curation, bibRecord, 000120

Detection of SARS coronavirus in patients with severe acute respiratory syndrome by conventional and real-time quantitative reverse transcription-PCR assays

Identifieur interne : 000120 ( PascalFrancis/Curation ); précédent : 000119; suivant : 000121

Detection of SARS coronavirus in patients with severe acute respiratory syndrome by conventional and real-time quantitative reverse transcription-PCR assays

Auteurs : Leo L. M. Poon [Hong Kong] ; KWOK HUNG CHAN [Hong Kong] ; ON KEI WONG [Hong Kong] ; Timothy K. W. Cheung [Hong Kong] ; Iris Ng [Hong Kong] ; BOJIAN ZHENG [Hong Kong] ; WING HONG SETO [Hong Kong] ; KWOK YUNG YUEN [Hong Kong] ; YI GUAN [Hong Kong] ; Joseph S. M. Peiris [Hong Kong]

Source :

Clinical chemistry : (Baltimore, Md.) [ 0009-9147 ] ; 2004.

RBID : Pascal:04-0330063

Descripteurs français

Pascal (Inist)
- Coronavirus, Homme, Temps réel, Analyse quantitative, Réaction chaîne polymérase RT, Analyse biochimique, Biochimie, Biologie clinique, Syndrome respiratoire aigu sévère.
Wicri :
- topic : Homme, Analyse quantitative, Biochimie.

English descriptors

KwdEn :
- Biochemical analysis, Biochemistry, Clinical biology, Coronavirus, Human, Quantitative analysis, Real time, Reverse transcription polymerase chain reaction, Severe acute respiratory syndrome.

Abstract

Background: A novel coronavirus (CoV) was recently identified as the agent for severe acute respiratory syndrome (SARS). We compared the abilities of conventional and real-time reverse transcription-PCR (RT-PCR) assays to detect SARS CoV in clinical specimens. Methods: RNA samples isolated from nasopharyngeal aspirate (NPA; n = 170) and stool (n = 44) were reverse-transcribed and tested by our in-house conventional RT-PCR assay. We selected 98 NPA and 37 stool samples collected at different times after the onset of disease and tested them in a real-time quantitative RT-PCR specific for the open reading frame (ORF) 1b region of SARS CoV. Detection rates for the conventional and real-time quantitative RT-PCR assays were compared. To investigate the nature of viral RNA molecules in these clinical samples, we determined copy numbers of ORF 1b and nucleocapsid (N) gene sequences of SARS CoV. Results: The quantitative real-time RT-PCR assay was more sensitive than the conventional RT-PCR assay for detecting SARS CoV in samples collected early in the course of the disease. Real-time assays targeted at the ORF 1b region and the N gene revealed that copy numbers of ORF 1b and N gene sequences in clinical samples were similar. Conclusions: NPA and stool samples can be used for early diagnosis of SARS. The real-time quantitative RT-PCR assay for SARS CoV is potentially useful for early detection of SARS CoV. Our results suggest that genomic RNA is the predominant viral RNA species in clinical samples.

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A11	`01`	`1`		`@1 POON (Leo L. M.)`
A11	`02`	`1`		`@1 KWOK HUNG CHAN`
A11	`03`	`1`		`@1 ON KEI WONG`
A11	`04`	`1`		`@1 CHEUNG (Timothy K. W.)`
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A11	`09`	`1`		`@1 YI GUAN`
A11	`10`	`1`		`@1 PEIRIS (Joseph S. M.)`
A14	`01`			`@1 Department of Microbiology, The University of Hong Kong @3 HKG @Z 1 aut. @Z 3 aut. @Z 4 aut. @Z 5 aut. @Z 6 aut. @Z 8 aut. @Z 9 aut. @Z 10 aut.`
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C01	`01`		`ENG`	@0 Background: A novel coronavirus (CoV) was recently identified as the agent for severe acute respiratory syndrome (SARS). We compared the abilities of conventional and real-time reverse transcription-PCR (RT-PCR) assays to detect SARS CoV in clinical specimens. Methods: RNA samples isolated from nasopharyngeal aspirate (NPA; n = 170) and stool (n = 44) were reverse-transcribed and tested by our in-house conventional RT-PCR assay. We selected 98 NPA and 37 stool samples collected at different times after the onset of disease and tested them in a real-time quantitative RT-PCR specific for the open reading frame (ORF) 1b region of SARS CoV. Detection rates for the conventional and real-time quantitative RT-PCR assays were compared. To investigate the nature of viral RNA molecules in these clinical samples, we determined copy numbers of ORF 1b and nucleocapsid (N) gene sequences of SARS CoV. Results: The quantitative real-time RT-PCR assay was more sensitive than the conventional RT-PCR assay for detecting SARS CoV in samples collected early in the course of the disease. Real-time assays targeted at the ORF 1b region and the N gene revealed that copy numbers of ORF 1b and N gene sequences in clinical samples were similar. Conclusions: NPA and stool samples can be used for early diagnosis of SARS. The real-time quantitative RT-PCR assay for SARS CoV is potentially useful for early detection of SARS CoV. Our results suggest that genomic RNA is the predominant viral RNA species in clinical samples.
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Pascal:04-0330063

Le document en format XML

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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biochemical analysis</term>
<term>Biochemistry</term>
<term>Clinical biology</term>
<term>Coronavirus</term>
<term>Human</term>
<term>Quantitative analysis</term>
<term>Real time</term>
<term>Reverse transcription polymerase chain reaction</term>
<term>Severe acute respiratory syndrome</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Coronavirus</term>
<term>Homme</term>
<term>Temps réel</term>
<term>Analyse quantitative</term>
<term>Réaction chaîne polymérase RT</term>
<term>Analyse biochimique</term>
<term>Biochimie</term>
<term>Biologie clinique</term>
<term>Syndrome respiratoire aigu sévère</term>
</keywords>
<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Homme</term>
<term>Analyse quantitative</term>
<term>Biochimie</term>
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<front><div type="abstract" xml:lang="en">Background: A novel coronavirus (CoV) was recently identified as the agent for severe acute respiratory syndrome (SARS). We compared the abilities of conventional and real-time reverse transcription-PCR (RT-PCR) assays to detect SARS CoV in clinical specimens. Methods: RNA samples isolated from nasopharyngeal aspirate (NPA; n = 170) and stool (n = 44) were reverse-transcribed and tested by our in-house conventional RT-PCR assay. We selected 98 NPA and 37 stool samples collected at different times after the onset of disease and tested them in a real-time quantitative RT-PCR specific for the open reading frame (ORF) 1b region of SARS CoV. Detection rates for the conventional and real-time quantitative RT-PCR assays were compared. To investigate the nature of viral RNA molecules in these clinical samples, we determined copy numbers of ORF 1b and nucleocapsid (N) gene sequences of SARS CoV. Results: The quantitative real-time RT-PCR assay was more sensitive than the conventional RT-PCR assay for detecting SARS CoV in samples collected early in the course of the disease. Real-time assays targeted at the ORF 1b region and the N gene revealed that copy numbers of ORF 1b and N gene sequences in clinical samples were similar. Conclusions: NPA and stool samples can be used for early diagnosis of SARS. The real-time quantitative RT-PCR assay for SARS CoV is potentially useful for early detection of SARS CoV. Our results suggest that genomic RNA is the predominant viral RNA species in clinical samples.</div>
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<fA03 i2="1"><s0>Clin. chem. : (Baltim. Md.)</s0>
</fA03>
<fA05><s2>50</s2>
</fA05>
<fA06><s2>1</s2>
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<fA08 i1="01" i2="1" l="ENG"><s1>Detection of SARS coronavirus in patients with severe acute respiratory syndrome by conventional and real-time quantitative reverse transcription-PCR assays</s1>
</fA08>
<fA11 i1="01" i2="1"><s1>POON (Leo L. M.)</s1>
</fA11>
<fA11 i1="02" i2="1"><s1>KWOK HUNG CHAN</s1>
</fA11>
<fA11 i1="03" i2="1"><s1>ON KEI WONG</s1>
</fA11>
<fA11 i1="04" i2="1"><s1>CHEUNG (Timothy K. W.)</s1>
</fA11>
<fA11 i1="05" i2="1"><s1>NG (Iris)</s1>
</fA11>
<fA11 i1="06" i2="1"><s1>BOJIAN ZHENG</s1>
</fA11>
<fA11 i1="07" i2="1"><s1>WING HONG SETO</s1>
</fA11>
<fA11 i1="08" i2="1"><s1>KWOK YUNG YUEN</s1>
</fA11>
<fA11 i1="09" i2="1"><s1>YI GUAN</s1>
</fA11>
<fA11 i1="10" i2="1"><s1>PEIRIS (Joseph S. M.)</s1>
</fA11>
<fA14 i1="01"><s1>Department of Microbiology, The University of Hong Kong</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
</fA14>
<fA14 i1="02"><s1>Department of Microbiology, Queen Mary Hospital</s1>
<s3>HKG</s3>
<sZ>2 aut.</sZ>
<sZ>7 aut.</sZ>
</fA14>
<fA20><s1>67-72</s1>
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<fA60><s1>P</s1>
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<fA61><s0>A</s0>
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<fA66 i1="01"><s0>USA</s0>
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<fC01 i1="01" l="ENG"><s0>Background: A novel coronavirus (CoV) was recently identified as the agent for severe acute respiratory syndrome (SARS). We compared the abilities of conventional and real-time reverse transcription-PCR (RT-PCR) assays to detect SARS CoV in clinical specimens. Methods: RNA samples isolated from nasopharyngeal aspirate (NPA; n = 170) and stool (n = 44) were reverse-transcribed and tested by our in-house conventional RT-PCR assay. We selected 98 NPA and 37 stool samples collected at different times after the onset of disease and tested them in a real-time quantitative RT-PCR specific for the open reading frame (ORF) 1b region of SARS CoV. Detection rates for the conventional and real-time quantitative RT-PCR assays were compared. To investigate the nature of viral RNA molecules in these clinical samples, we determined copy numbers of ORF 1b and nucleocapsid (N) gene sequences of SARS CoV. Results: The quantitative real-time RT-PCR assay was more sensitive than the conventional RT-PCR assay for detecting SARS CoV in samples collected early in the course of the disease. Real-time assays targeted at the ORF 1b region and the N gene revealed that copy numbers of ORF 1b and N gene sequences in clinical samples were similar. Conclusions: NPA and stool samples can be used for early diagnosis of SARS. The real-time quantitative RT-PCR assay for SARS CoV is potentially useful for early detection of SARS CoV. Our results suggest that genomic RNA is the predominant viral RNA species in clinical samples.</s0>
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Detection of SARS coronavirus in patients with severe acute respiratory syndrome by conventional and real-time quantitative reverse transcription-PCR assays

Detection of SARS coronavirus in patients with severe acute respiratory syndrome by conventional and real-time quantitative reverse transcription-PCR assays

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