SrasV1, PascalFrancis, Curation, bibRecord, 000097

Detection of specific antibodies to severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein for serodiagnosis of SARS coronavirus pneumonia

Identifieur interne : 000097 ( PascalFrancis/Curation ); précédent : 000096; suivant : 000098

Detection of specific antibodies to severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein for serodiagnosis of SARS coronavirus pneumonia

Auteurs : Patrick C. Y. Woo [Hong Kong] ; Susanna K. P. Lau [Hong Kong] ; Beatrice H. L. Wong [Hong Kong] ; Hoi-Wah Tsoi [Hong Kong] ; Ami M. Y. Fung [Hong Kong] ; Kwok-Hung Chan [Hong Kong] ; Victoria K. P. Tam [Hong Kong] ; J. S. Malik Peiris [Hong Kong] ; Kwok-Yung Yuen [Hong Kong]

Source :

Journal of clinical microbiology : (Print) [ 0095-1137 ] ; 2004.

RBID : Pascal:04-0283850

Descripteurs français

Pascal (Inist)
- Coronavirus, Détection, Anticorps, Nucléocapside, Protéine, Microbiologie, Pneumonie, Syndrome respiratoire aigu sévère.

English descriptors

KwdEn :
- Antibody, Coronavirus, Detection, Microbiology, Nucleocapsid, Pneumonia, Protein, Severe acute respiratory syndrome.

Abstract

We report the evaluation of recombinant severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) nucleocapsid protein enzyme-linked immunosorbent assay (ELISA)-based antibody tests for serodiagnosis of SARS-CoV pneumonia and compare the sensitivities and specificities of this ELISA for detection of immunoglobulin G (IgG), IgM, IgA, and their combinations with serum samples from 149 healthy blood donors who donated blood 3 years ago as controls and 106 SARS-CoV pneumonia patients in Hong Kong. The specificities of the ELISA for IgG, IgM, and IgA detection were 95.3, 96.6, and 96.6%, respectively, with corresponding sensitivities of 943, 59.4, and 60.4%, respectively. The present ELISA appears to be a sensitive test for serodiagnosis of SARS-CoV pneumonia, is much more economical and less labor-intensive than the indirect immunofluorescence assay, and does not require cultivation of SARS-CoV.

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A02	`01`			`@0 JCMIDW`
A03		`1`		`@0 J. clin. microbiol. : (Print)`
A05				`@2 42`
A06				`@2 5`
A08	`01`	`1`	`ENG`	`@1 Detection of specific antibodies to severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein for serodiagnosis of SARS coronavirus pneumonia`
A11	`01`	`1`		`@1 WOO (Patrick C. Y.)`
A11	`02`	`1`		`@1 LAU (Susanna K. P.)`
A11	`03`	`1`		`@1 WONG (Beatrice H. L.)`
A11	`04`	`1`		`@1 TSOI (Hoi-Wah)`
A11	`05`	`1`		`@1 FUNG (Ami M. Y.)`
A11	`06`	`1`		`@1 CHAN (Kwok-Hung)`
A11	`07`	`1`		`@1 TAM (Victoria K. P.)`
A11	`08`	`1`		`@1 PEIRIS (J. S. Malik)`
A11	`09`	`1`		`@1 YUEN (Kwok-Yung)`
A14	`01`			`@1 Department of Microbiology, University of Hong Kong, Queen Mary Hospital @3 HKG @Z 1 aut. @Z 2 aut. @Z 3 aut. @Z 4 aut. @Z 5 aut. @Z 6 aut. @Z 7 aut. @Z 8 aut. @Z 9 aut.`
A20				`@1 2306-2309`
A21				`@1 2004`
A23	`01`			`@0 ENG`
A43	`01`			`@1 INIST @2 17088 @5 354000111957410800`
A44				`@0 0000 @1 © 2004 INIST-CNRS. All rights reserved.`
A45				`@0 23 ref.`
A47	`01`	`1`		`@0 04-0283850`
A60				`@1 P`
A61				`@0 A`
A64	`01`	`1`		`@0 Journal of clinical microbiology : (Print)`
A66	`01`			`@0 USA`
C01	`01`		`ENG`	@0 We report the evaluation of recombinant severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) nucleocapsid protein enzyme-linked immunosorbent assay (ELISA)-based antibody tests for serodiagnosis of SARS-CoV pneumonia and compare the sensitivities and specificities of this ELISA for detection of immunoglobulin G (IgG), IgM, IgA, and their combinations with serum samples from 149 healthy blood donors who donated blood 3 years ago as controls and 106 SARS-CoV pneumonia patients in Hong Kong. The specificities of the ELISA for IgG, IgM, and IgA detection were 95.3, 96.6, and 96.6%, respectively, with corresponding sensitivities of 943, 59.4, and 60.4%, respectively. The present ELISA appears to be a sensitive test for serodiagnosis of SARS-CoV pneumonia, is much more economical and less labor-intensive than the indirect immunofluorescence assay, and does not require cultivation of SARS-CoV.
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C03	`01`	`X`	`FRE`	`@0 Coronavirus @2 NW @5 01`
C03	`01`	`X`	`ENG`	`@0 Coronavirus @2 NW @5 01`
C03	`01`	`X`	`SPA`	`@0 Coronavirus @2 NW @5 01`
C03	`02`	`X`	`FRE`	`@0 Détection @5 05`
C03	`02`	`X`	`ENG`	`@0 Detection @5 05`
C03	`02`	`X`	`SPA`	`@0 Detección @5 05`
C03	`03`	`X`	`FRE`	`@0 Anticorps @5 06`
C03	`03`	`X`	`ENG`	`@0 Antibody @5 06`
C03	`03`	`X`	`SPA`	`@0 Anticuerpo @5 06`
C03	`04`	`X`	`FRE`	`@0 Nucléocapside @5 07`
C03	`04`	`X`	`ENG`	`@0 Nucleocapsid @5 07`
C03	`04`	`X`	`SPA`	`@0 Nucleocápside @5 07`
C03	`05`	`X`	`FRE`	`@0 Protéine @5 08`
C03	`05`	`X`	`ENG`	`@0 Protein @5 08`
C03	`05`	`X`	`SPA`	`@0 Proteína @5 08`
C03	`06`	`X`	`FRE`	`@0 Microbiologie @5 09`
C03	`06`	`X`	`ENG`	`@0 Microbiology @5 09`
C03	`06`	`X`	`SPA`	`@0 Microbiología @5 09`
C03	`07`	`X`	`FRE`	`@0 Pneumonie @5 14`
C03	`07`	`X`	`ENG`	`@0 Pneumonia @5 14`
C03	`07`	`X`	`SPA`	`@0 Neumonía @5 14`
C03	`08`	`X`	`FRE`	`@0 Syndrome respiratoire aigu sévère @4 CD @5 96`
C03	`08`	`X`	`ENG`	`@0 Severe acute respiratory syndrome @4 CD @5 96`
C03	`08`	`X`	`SPA`	`@0 Síndrome respiratorio agudo severo @4 CD @5 96`
C07	`01`	`X`	`FRE`	`@0 Coronaviridae @2 NW`
C07	`01`	`X`	`ENG`	`@0 Coronaviridae @2 NW`
C07	`01`	`X`	`SPA`	`@0 Coronaviridae @2 NW`
C07	`02`	`X`	`FRE`	`@0 Nidovirales @2 NW`
C07	`02`	`X`	`ENG`	`@0 Nidovirales @2 NW`
C07	`02`	`X`	`SPA`	`@0 Nidovirales @2 NW`
C07	`03`	`X`	`FRE`	`@0 Virus @2 NW`
C07	`03`	`X`	`ENG`	`@0 Virus @2 NW`
C07	`03`	`X`	`SPA`	`@0 Virus @2 NW`
C07	`04`	`X`	`FRE`	`@0 Appareil respiratoire pathologie @5 19`
C07	`04`	`X`	`ENG`	`@0 Respiratory disease @5 19`
C07	`04`	`X`	`SPA`	`@0 Aparato respiratorio patología @5 19`
C07	`05`	`X`	`FRE`	`@0 Poumon pathologie @5 20`
C07	`05`	`X`	`ENG`	`@0 Lung disease @5 20`
C07	`05`	`X`	`SPA`	`@0 Pulmón patología @5 20`
C07	`06`	`X`	`FRE`	`@0 Virose @2 NM @5 21`
C07	`06`	`X`	`ENG`	`@0 Viral disease @2 NM @5 21`
C07	`06`	`X`	`SPA`	`@0 Virosis @2 NM @5 21`
C07	`07`	`X`	`FRE`	`@0 Infection @2 NM`
C07	`07`	`X`	`ENG`	`@0 Infection @2 NM`
C07	`07`	`X`	`SPA`	`@0 Infección @2 NM`
N21				`@1 173`
N44	`01`			`@1 OTO`
N82				`@1 OTO`

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Pascal:04-0283850

Le document en format XML

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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibody</term>
<term>Coronavirus</term>
<term>Detection</term>
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<term>Nucleocapsid</term>
<term>Pneumonia</term>
<term>Protein</term>
<term>Severe acute respiratory syndrome</term>
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<keywords scheme="Pascal" xml:lang="fr"><term>Coronavirus</term>
<term>Détection</term>
<term>Anticorps</term>
<term>Nucléocapside</term>
<term>Protéine</term>
<term>Microbiologie</term>
<term>Pneumonie</term>
<term>Syndrome respiratoire aigu sévère</term>
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</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">We report the evaluation of recombinant severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) nucleocapsid protein enzyme-linked immunosorbent assay (ELISA)-based antibody tests for serodiagnosis of SARS-CoV pneumonia and compare the sensitivities and specificities of this ELISA for detection of immunoglobulin G (IgG), IgM, IgA, and their combinations with serum samples from 149 healthy blood donors who donated blood 3 years ago as controls and 106 SARS-CoV pneumonia patients in Hong Kong. The specificities of the ELISA for IgG, IgM, and IgA detection were 95.3, 96.6, and 96.6%, respectively, with corresponding sensitivities of 943, 59.4, and 60.4%, respectively. The present ELISA appears to be a sensitive test for serodiagnosis of SARS-CoV pneumonia, is much more economical and less labor-intensive than the indirect immunofluorescence assay, and does not require cultivation of SARS-CoV.</div>
</front>
</TEI>
<inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0095-1137</s0>
</fA01>
<fA02 i1="01"><s0>JCMIDW</s0>
</fA02>
<fA03 i2="1"><s0>J. clin. microbiol. : (Print)</s0>
</fA03>
<fA05><s2>42</s2>
</fA05>
<fA06><s2>5</s2>
</fA06>
<fA08 i1="01" i2="1" l="ENG"><s1>Detection of specific antibodies to severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein for serodiagnosis of SARS coronavirus pneumonia</s1>
</fA08>
<fA11 i1="01" i2="1"><s1>WOO (Patrick C. Y.)</s1>
</fA11>
<fA11 i1="02" i2="1"><s1>LAU (Susanna K. P.)</s1>
</fA11>
<fA11 i1="03" i2="1"><s1>WONG (Beatrice H. L.)</s1>
</fA11>
<fA11 i1="04" i2="1"><s1>TSOI (Hoi-Wah)</s1>
</fA11>
<fA11 i1="05" i2="1"><s1>FUNG (Ami M. Y.)</s1>
</fA11>
<fA11 i1="06" i2="1"><s1>CHAN (Kwok-Hung)</s1>
</fA11>
<fA11 i1="07" i2="1"><s1>TAM (Victoria K. P.)</s1>
</fA11>
<fA11 i1="08" i2="1"><s1>PEIRIS (J. S. Malik)</s1>
</fA11>
<fA11 i1="09" i2="1"><s1>YUEN (Kwok-Yung)</s1>
</fA11>
<fA14 i1="01"><s1>Department of Microbiology, University of Hong Kong, Queen Mary Hospital</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
</fA14>
<fA20><s1>2306-2309</s1>
</fA20>
<fA21><s1>2004</s1>
</fA21>
<fA23 i1="01"><s0>ENG</s0>
</fA23>
<fA43 i1="01"><s1>INIST</s1>
<s2>17088</s2>
<s5>354000111957410800</s5>
</fA43>
<fA44><s0>0000</s0>
<s1>© 2004 INIST-CNRS. All rights reserved.</s1>
</fA44>
<fA45><s0>23 ref.</s0>
</fA45>
<fA47 i1="01" i2="1"><s0>04-0283850</s0>
</fA47>
<fA60><s1>P</s1>
</fA60>
<fA61><s0>A</s0>
</fA61>
<fA64 i1="01" i2="1"><s0>Journal of clinical microbiology : (Print)</s0>
</fA64>
<fA66 i1="01"><s0>USA</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>We report the evaluation of recombinant severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) nucleocapsid protein enzyme-linked immunosorbent assay (ELISA)-based antibody tests for serodiagnosis of SARS-CoV pneumonia and compare the sensitivities and specificities of this ELISA for detection of immunoglobulin G (IgG), IgM, IgA, and their combinations with serum samples from 149 healthy blood donors who donated blood 3 years ago as controls and 106 SARS-CoV pneumonia patients in Hong Kong. The specificities of the ELISA for IgG, IgM, and IgA detection were 95.3, 96.6, and 96.6%, respectively, with corresponding sensitivities of 943, 59.4, and 60.4%, respectively. The present ELISA appears to be a sensitive test for serodiagnosis of SARS-CoV pneumonia, is much more economical and less labor-intensive than the indirect immunofluorescence assay, and does not require cultivation of SARS-CoV.</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>002A05C10</s0>
</fC02>
<fC02 i1="02" i2="X"><s0>002B05</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Détection</s0>
<s5>05</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Detection</s0>
<s5>05</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Detección</s0>
<s5>05</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Anticorps</s0>
<s5>06</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Antibody</s0>
<s5>06</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Anticuerpo</s0>
<s5>06</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Nucléocapside</s0>
<s5>07</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Nucleocapsid</s0>
<s5>07</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Nucleocápside</s0>
<s5>07</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Protéine</s0>
<s5>08</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Protein</s0>
<s5>08</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Proteína</s0>
<s5>08</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Microbiologie</s0>
<s5>09</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Microbiology</s0>
<s5>09</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Microbiología</s0>
<s5>09</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE"><s0>Pneumonie</s0>
<s5>14</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG"><s0>Pneumonia</s0>
<s5>14</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA"><s0>Neumonía</s0>
<s5>14</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE"><s0>Syndrome respiratoire aigu sévère</s0>
<s4>CD</s4>
<s5>96</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG"><s0>Severe acute respiratory syndrome</s0>
<s4>CD</s4>
<s5>96</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA"><s0>Síndrome respiratorio agudo severo</s0>
<s4>CD</s4>
<s5>96</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Appareil respiratoire pathologie</s0>
<s5>19</s5>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Respiratory disease</s0>
<s5>19</s5>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Aparato respiratorio patología</s0>
<s5>19</s5>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Poumon pathologie</s0>
<s5>20</s5>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Lung disease</s0>
<s5>20</s5>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Pulmón patología</s0>
<s5>20</s5>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Virose</s0>
<s2>NM</s2>
<s5>21</s5>
</fC07>
<fC07 i1="06" i2="X" l="ENG"><s0>Viral disease</s0>
<s2>NM</s2>
<s5>21</s5>
</fC07>
<fC07 i1="06" i2="X" l="SPA"><s0>Virosis</s0>
<s2>NM</s2>
<s5>21</s5>
</fC07>
<fC07 i1="07" i2="X" l="FRE"><s0>Infection</s0>
<s2>NM</s2>
</fC07>
<fC07 i1="07" i2="X" l="ENG"><s0>Infection</s0>
<s2>NM</s2>
</fC07>
<fC07 i1="07" i2="X" l="SPA"><s0>Infección</s0>
<s2>NM</s2>
</fC07>
<fN21><s1>173</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
</standard>
</inist>
</record>

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Detection of specific antibodies to severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein for serodiagnosis of SARS coronavirus pneumonia

Detection of specific antibodies to severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein for serodiagnosis of SARS coronavirus pneumonia

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