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Detection of severe acute respiratory syndrome coronavirus in blood of infected patients

Identifieur interne : 000072 ( PascalFrancis/Curation ); précédent : 000071; suivant : 000073

Detection of severe acute respiratory syndrome coronavirus in blood of infected patients

Auteurs : Lisa F. P. Ng [Singapour] ; Michelle Wong [Singapour] ; Susie Koh [Singapour] ; Eng-Eong Ooi [Singapour] ; King-Fai Tang [Singapour] ; Hoe-Nam Leong [Singapour] ; Ai-Ee Ling [Singapour] ; Lora V. Agathe [Singapour] ; Jenny Tan [Singapour] ; Edison T. Liu [Singapour] ; Ee-Chee Ren [Singapour] ; Lee-Ching Ng [Singapour] ; Martin L. Hibberd [Singapour]

Source :

RBID : Pascal:04-0200626

Descripteurs français

English descriptors

Abstract

Severe acute respiratory syndrome (SARS) has caused major outbreaks worldwide, resulting in an urgent need to obtain sensitive and accurate diagnosis of this disease. PCR-based detection methods were developed for use on a variety of samples, including blood. Eighteen subjects were investigated, and results indicated that blood samples contain sufficient virus for detection by using quantitative real-time PCR.
pA  
A01 01  1    @0 0095-1137
A02 01      @0 JCMIDW
A03   1    @0 J. clin. microbiol. : (Print)
A05       @2 42
A06       @2 1
A08 01  1  ENG  @1 Detection of severe acute respiratory syndrome coronavirus in blood of infected patients
A11 01  1    @1 NG (Lisa F. P.)
A11 02  1    @1 WONG (Michelle)
A11 03  1    @1 KOH (Susie)
A11 04  1    @1 OOI (Eng-Eong)
A11 05  1    @1 TANG (King-Fai)
A11 06  1    @1 LEONG (Hoe-Nam)
A11 07  1    @1 LING (Ai-Ee)
A11 08  1    @1 AGATHE (Lora V.)
A11 09  1    @1 TAN (Jenny)
A11 10  1    @1 LIU (Edison T.)
A11 11  1    @1 REN (Ee-Chee)
A11 12  1    @1 NG (Lee-Ching)
A11 13  1    @1 HIBBERD (Martin L.)
A14 01      @1 Genome Institute of Singapore, National University of Singapore @2 Singapore @3 SGP @Z 1 aut. @Z 8 aut. @Z 9 aut. @Z 10 aut. @Z 11 aut. @Z 13 aut.
A14 02      @1 DSO National Laboratories, National University of Singapore @2 Singapore @3 SGP @Z 2 aut. @Z 3 aut. @Z 12 aut.
A14 03      @1 Environmental Health Institute, National Environment Agency, National University of Singapore @2 Singapore @3 SGP @Z 4 aut. @Z 5 aut.
A14 04      @1 Tan Tock Seng Hospital, National University of Singapore @2 Singapore @3 SGP @Z 6 aut.
A14 05      @1 Department of Pathology, Singapore General Hospital, National University of Singapore @2 Singapore @3 SGP @Z 7 aut.
A14 06      @1 Department of Microbiology, National University of Singapore @2 Singapore @3 SGP @Z 11 aut.
A20       @1 347-350
A21       @1 2004
A23 01      @0 ENG
A43 01      @1 INIST @2 17088 @5 354000111456540530
A44       @0 0000 @1 © 2004 INIST-CNRS. All rights reserved.
A45       @0 10 ref.
A47 01  1    @0 04-0200626
A60       @1 P
A61       @0 A
A64 01  1    @0 Journal of clinical microbiology : (Print)
A66 01      @0 USA
C01 01    ENG  @0 Severe acute respiratory syndrome (SARS) has caused major outbreaks worldwide, resulting in an urgent need to obtain sensitive and accurate diagnosis of this disease. PCR-based detection methods were developed for use on a variety of samples, including blood. Eighteen subjects were investigated, and results indicated that blood samples contain sufficient virus for detection by using quantitative real-time PCR.
C02 01  X    @0 002A05C10
C02 02  X    @0 002B01
C02 03  X    @0 002B05
C03 01  X  FRE  @0 Coronavirus @2 NW @5 01
C03 01  X  ENG  @0 Coronavirus @2 NW @5 01
C03 01  X  SPA  @0 Coronavirus @2 NW @5 01
C03 02  X  FRE  @0 Homme @5 02
C03 02  X  ENG  @0 Human @5 02
C03 02  X  SPA  @0 Hombre @5 02
C03 03  X  FRE  @0 Détection @5 05
C03 03  X  ENG  @0 Detection @5 05
C03 03  X  SPA  @0 Detección @5 05
C03 04  X  FRE  @0 Sang @5 06
C03 04  X  ENG  @0 Blood @5 06
C03 04  X  SPA  @0 Sangre @5 06
C03 05  X  FRE  @0 Infection @2 NM @5 07
C03 05  X  ENG  @0 Infection @2 NM @5 07
C03 05  X  SPA  @0 Infección @2 NM @5 07
C03 06  X  FRE  @0 Médecine tropicale @5 08
C03 06  X  ENG  @0 Tropical medicine @5 08
C03 06  X  SPA  @0 Medicina tropical @5 08
C03 07  X  FRE  @0 Microbiologie @5 09
C03 07  X  ENG  @0 Microbiology @5 09
C03 07  X  SPA  @0 Microbiología @5 09
C03 08  X  FRE  @0 Syndrome respiratoire aigu sévère @4 CD @5 96
C03 08  X  ENG  @0 Severe acute respiratory syndrome @4 CD @5 96
C03 08  X  SPA  @0 Síndrome respiratorio agudo severo @4 CD @5 96
C07 01  X  FRE  @0 Coronaviridae @2 NW
C07 01  X  ENG  @0 Coronaviridae @2 NW
C07 01  X  SPA  @0 Coronaviridae @2 NW
C07 02  X  FRE  @0 Nidovirales @2 NW
C07 02  X  ENG  @0 Nidovirales @2 NW
C07 02  X  SPA  @0 Nidovirales @2 NW
C07 03  X  FRE  @0 Virus @2 NW
C07 03  X  ENG  @0 Virus @2 NW
C07 03  X  SPA  @0 Virus @2 NW
C07 04  X  FRE  @0 Appareil respiratoire pathologie @5 19
C07 04  X  ENG  @0 Respiratory disease @5 19
C07 04  X  SPA  @0 Aparato respiratorio patología @5 19
C07 05  X  FRE  @0 Poumon pathologie @5 20
C07 05  X  ENG  @0 Lung disease @5 20
C07 05  X  SPA  @0 Pulmón patología @5 20
C07 06  X  FRE  @0 Virose @2 NM @5 21
C07 06  X  ENG  @0 Viral disease @2 NM @5 21
C07 06  X  SPA  @0 Virosis @2 NM @5 21
N21       @1 138
N82       @1 OTO

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Pascal:04-0200626

Le document en format XML

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<title level="j" type="main">Journal of clinical microbiology : (Print)</title>
<title level="j" type="abbreviated">J. clin. microbiol. : (Print)</title>
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<title level="j" type="main">Journal of clinical microbiology : (Print)</title>
<title level="j" type="abbreviated">J. clin. microbiol. : (Print)</title>
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<term>Blood</term>
<term>Coronavirus</term>
<term>Detection</term>
<term>Human</term>
<term>Infection</term>
<term>Microbiology</term>
<term>Severe acute respiratory syndrome</term>
<term>Tropical medicine</term>
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<term>Coronavirus</term>
<term>Homme</term>
<term>Détection</term>
<term>Sang</term>
<term>Infection</term>
<term>Médecine tropicale</term>
<term>Microbiologie</term>
<term>Syndrome respiratoire aigu sévère</term>
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<div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) has caused major outbreaks worldwide, resulting in an urgent need to obtain sensitive and accurate diagnosis of this disease. PCR-based detection methods were developed for use on a variety of samples, including blood. Eighteen subjects were investigated, and results indicated that blood samples contain sufficient virus for detection by using quantitative real-time PCR.</div>
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