Curation
PascalFrancis
Racine
Curation
Checkpoint
PascalFrancis
Racine
PascalFrancis
Exploration
Accueil
Racine
Racine

Serveur d'exploration SRAS

Attention, ce site est en cours de développement !
Attention, site généré par des moyens informatiques à partir de corpus bruts.
Les informations ne sont donc pas validées.

Preclinical evaluation of two real-time, reverse transcription-PCR assays for detection of the severe acute respiratory syndrome coronavirus

Identifieur interne : 000070 ( PascalFrancis/Curation ); précédent : 000069; suivant : 000071

Preclinical evaluation of two real-time, reverse transcription-PCR assays for detection of the severe acute respiratory syndrome coronavirus

Auteurs : Adam M. Bressler [États-Unis] ; Frederick S. Nolte

Source :

RBID : Pascal:04-0195367

Descripteurs français

English descriptors

Abstract

We verified the analytical performance characteristics of a previously described real-time reverse transcription-PCR (RT-PCR) assay targeting the open reading frame (ORF) 1b region of the severe acute respiratory syndrome coronavirus (SARS-CoV) with RNA transcripts. We then compared it to a novel nucleocapsid gene real-time RT-PCR assay with genomic RNA. The assays differed only in the primer and probe sequences and final concentrations. A commercially available armored RNA (Ambion, Austin, Tex.) was evaluated as positive control for the ORF 1b assay. The analytical sensitivity, reproducibility, amplification efficiency, and dynamic range of the assays were similar. Both were specific for SARS-CoV as determined by testing against human CoV 229E and OC43, specimens from patients without SARS, and by BLAST searches of GenBank for primer and probe sequence homology. The armored RNA was found to be a suitable positive control for the ORF 1b assay that could be reliably recovered and amplified from a variety of clinical specimens.
pA  
A01 01  1    @0 0095-1137
A02 01      @0 JCMIDW
A03   1    @0 J. clin. microbiol. : (Print)
A05       @2 42
A06       @2 3
A08 01  1  ENG  @1 Preclinical evaluation of two real-time, reverse transcription-PCR assays for detection of the severe acute respiratory syndrome coronavirus
A11 01  1    @1 BRESSLER (Adam M.)
A11 02  1    @1 NOLTE (Frederick S.)
A14 01      @1 Departments of Medicine (Infectious Diseases) and Pathology and Laboratory Medicine, Emory University School of Medicine @2 Atlanta, Georgia @3 USA
A20       @1 987-991
A21       @1 2004
A23 01      @0 ENG
A43 01      @1 INIST @2 17088 @5 354000119339680070
A44       @0 0000 @1 © 2004 INIST-CNRS. All rights reserved.
A45       @0 12 ref.
A47 01  1    @0 04-0195367
A60       @1 P
A61       @0 A
A64 01  1    @0 Journal of clinical microbiology : (Print)
A66 01      @0 USA
C01 01    ENG  @0 We verified the analytical performance characteristics of a previously described real-time reverse transcription-PCR (RT-PCR) assay targeting the open reading frame (ORF) 1b region of the severe acute respiratory syndrome coronavirus (SARS-CoV) with RNA transcripts. We then compared it to a novel nucleocapsid gene real-time RT-PCR assay with genomic RNA. The assays differed only in the primer and probe sequences and final concentrations. A commercially available armored RNA (Ambion, Austin, Tex.) was evaluated as positive control for the ORF 1b assay. The analytical sensitivity, reproducibility, amplification efficiency, and dynamic range of the assays were similar. Both were specific for SARS-CoV as determined by testing against human CoV 229E and OC43, specimens from patients without SARS, and by BLAST searches of GenBank for primer and probe sequence homology. The armored RNA was found to be a suitable positive control for the ORF 1b assay that could be reliably recovered and amplified from a variety of clinical specimens.
C02 01  X    @0 002A05
C02 02  X    @0 002B05
C02 03  X    @0 002A05C10
C03 01  X  FRE  @0 Coronavirus @2 NW @5 01
C03 01  X  ENG  @0 Coronavirus @2 NW @5 01
C03 01  X  SPA  @0 Coronavirus @2 NW @5 01
C03 02  X  FRE  @0 Temps réel @5 05
C03 02  X  ENG  @0 Real time @5 05
C03 02  X  SPA  @0 Tiempo real @5 05
C03 03  X  FRE  @0 Réaction chaîne polymérase RT @5 06
C03 03  X  ENG  @0 Reverse transcription polymerase chain reaction @5 06
C03 03  X  SPA  @0 Reacción cadena polimerasa transcripción inversa @5 06
C03 04  X  FRE  @0 Détection @5 07
C03 04  X  ENG  @0 Detection @5 07
C03 04  X  SPA  @0 Detección @5 07
C03 05  X  FRE  @0 Microbiologie @5 08
C03 05  X  ENG  @0 Microbiology @5 08
C03 05  X  SPA  @0 Microbiología @5 08
C03 06  X  FRE  @0 Syndrome respiratoire aigu sévère @4 CD @5 96
C03 06  X  ENG  @0 Severe acute respiratory syndrome @4 CD @5 96
C03 06  X  SPA  @0 Síndrome respiratorio agudo severo @4 CD @5 96
C07 01  X  FRE  @0 Coronaviridae @2 NW
C07 01  X  ENG  @0 Coronaviridae @2 NW
C07 01  X  SPA  @0 Coronaviridae @2 NW
C07 02  X  FRE  @0 Nidovirales @2 NW
C07 02  X  ENG  @0 Nidovirales @2 NW
C07 02  X  SPA  @0 Nidovirales @2 NW
C07 03  X  FRE  @0 Virus @2 NW
C07 03  X  ENG  @0 Virus @2 NW
C07 03  X  SPA  @0 Virus @2 NW
C07 04  X  FRE  @0 Appareil respiratoire pathologie @5 19
C07 04  X  ENG  @0 Respiratory disease @5 19
C07 04  X  SPA  @0 Aparato respiratorio patología @5 19
C07 05  X  FRE  @0 Poumon pathologie @5 20
C07 05  X  ENG  @0 Lung disease @5 20
C07 05  X  SPA  @0 Pulmón patología @5 20
C07 06  X  FRE  @0 Virose @2 NM @5 21
C07 06  X  ENG  @0 Viral disease @2 NM @5 21
C07 06  X  SPA  @0 Virosis @2 NM @5 21
C07 07  X  FRE  @0 Infection @2 NM
C07 07  X  ENG  @0 Infection @2 NM
C07 07  X  SPA  @0 Infección @2 NM
N21       @1 131
N82       @1 OTO

Links toward previous steps (curation, corpus...)

Links to Exploration step

Pascal:04-0195367

Le document en format XML

<record>
<TEI>
<teiHeader>
<fileDesc>
<titleStmt>
<title xml:lang="en" level="a">Preclinical evaluation of two real-time, reverse transcription-PCR assays for detection of the severe acute respiratory syndrome coronavirus</title>
<author>
<name sortKey="Bressler, Adam M" sort="Bressler, Adam M" uniqKey="Bressler A" first="Adam M." last="Bressler">Adam M. Bressler</name>
<affiliation wicri:level="1">
<inist:fA14 i1="01">
<s1>Departments of Medicine (Infectious Diseases) and Pathology and Laboratory Medicine, Emory University School of Medicine</s1>
<s2>Atlanta, Georgia</s2>
<s3>USA</s3>
</inist:fA14>
<country>États-Unis</country>
</affiliation>
</author>
<author>
<name sortKey="Nolte, Frederick S" sort="Nolte, Frederick S" uniqKey="Nolte F" first="Frederick S." last="Nolte">Frederick S. Nolte</name>
</author>
</titleStmt>
<publicationStmt>
<idno type="wicri:source">INIST</idno>
<idno type="inist">04-0195367</idno>
<date when="2004">2004</date>
<idno type="stanalyst">PASCAL 04-0195367 INIST</idno>
<idno type="RBID">Pascal:04-0195367</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000920</idno>
<idno type="wicri:Area/PascalFrancis/Curation">000070</idno>
</publicationStmt>
<sourceDesc>
<biblStruct>
<analytic>
<title xml:lang="en" level="a">Preclinical evaluation of two real-time, reverse transcription-PCR assays for detection of the severe acute respiratory syndrome coronavirus</title>
<author>
<name sortKey="Bressler, Adam M" sort="Bressler, Adam M" uniqKey="Bressler A" first="Adam M." last="Bressler">Adam M. Bressler</name>
<affiliation wicri:level="1">
<inist:fA14 i1="01">
<s1>Departments of Medicine (Infectious Diseases) and Pathology and Laboratory Medicine, Emory University School of Medicine</s1>
<s2>Atlanta, Georgia</s2>
<s3>USA</s3>
</inist:fA14>
<country>États-Unis</country>
</affiliation>
</author>
<author>
<name sortKey="Nolte, Frederick S" sort="Nolte, Frederick S" uniqKey="Nolte F" first="Frederick S." last="Nolte">Frederick S. Nolte</name>
</author>
</analytic>
<series>
<title level="j" type="main">Journal of clinical microbiology : (Print)</title>
<title level="j" type="abbreviated">J. clin. microbiol. : (Print)</title>
<idno type="ISSN">0095-1137</idno>
<imprint>
<date when="2004">2004</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt>
<title level="j" type="main">Journal of clinical microbiology : (Print)</title>
<title level="j" type="abbreviated">J. clin. microbiol. : (Print)</title>
<idno type="ISSN">0095-1137</idno>
</seriesStmt>
</fileDesc>
<profileDesc>
<textClass>
<keywords scheme="KwdEn" xml:lang="en">
<term>Coronavirus</term>
<term>Detection</term>
<term>Microbiology</term>
<term>Real time</term>
<term>Reverse transcription polymerase chain reaction</term>
<term>Severe acute respiratory syndrome</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr">
<term>Coronavirus</term>
<term>Temps réel</term>
<term>Réaction chaîne polymérase RT</term>
<term>Détection</term>
<term>Microbiologie</term>
<term>Syndrome respiratoire aigu sévère</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front>
<div type="abstract" xml:lang="en">We verified the analytical performance characteristics of a previously described real-time reverse transcription-PCR (RT-PCR) assay targeting the open reading frame (ORF) 1b region of the severe acute respiratory syndrome coronavirus (SARS-CoV) with RNA transcripts. We then compared it to a novel nucleocapsid gene real-time RT-PCR assay with genomic RNA. The assays differed only in the primer and probe sequences and final concentrations. A commercially available armored RNA (Ambion, Austin, Tex.) was evaluated as positive control for the ORF 1b assay. The analytical sensitivity, reproducibility, amplification efficiency, and dynamic range of the assays were similar. Both were specific for SARS-CoV as determined by testing against human CoV 229E and OC43, specimens from patients without SARS, and by BLAST searches of GenBank for primer and probe sequence homology. The armored RNA was found to be a suitable positive control for the ORF 1b assay that could be reliably recovered and amplified from a variety of clinical specimens.</div>
</front>
</TEI>
<inist>
<standard h6="B">
<pA>
<fA01 i1="01" i2="1">
<s0>0095-1137</s0>
</fA01>
<fA02 i1="01">
<s0>JCMIDW</s0>
</fA02>
<fA03 i2="1">
<s0>J. clin. microbiol. : (Print)</s0>
</fA03>
<fA05>
<s2>42</s2>
</fA05>
<fA06>
<s2>3</s2>
</fA06>
<fA08 i1="01" i2="1" l="ENG">
<s1>Preclinical evaluation of two real-time, reverse transcription-PCR assays for detection of the severe acute respiratory syndrome coronavirus</s1>
</fA08>
<fA11 i1="01" i2="1">
<s1>BRESSLER (Adam M.)</s1>
</fA11>
<fA11 i1="02" i2="1">
<s1>NOLTE (Frederick S.)</s1>
</fA11>
<fA14 i1="01">
<s1>Departments of Medicine (Infectious Diseases) and Pathology and Laboratory Medicine, Emory University School of Medicine</s1>
<s2>Atlanta, Georgia</s2>
<s3>USA</s3>
</fA14>
<fA20>
<s1>987-991</s1>
</fA20>
<fA21>
<s1>2004</s1>
</fA21>
<fA23 i1="01">
<s0>ENG</s0>
</fA23>
<fA43 i1="01">
<s1>INIST</s1>
<s2>17088</s2>
<s5>354000119339680070</s5>
</fA43>
<fA44>
<s0>0000</s0>
<s1>© 2004 INIST-CNRS. All rights reserved.</s1>
</fA44>
<fA45>
<s0>12 ref.</s0>
</fA45>
<fA47 i1="01" i2="1">
<s0>04-0195367</s0>
</fA47>
<fA60>
<s1>P</s1>
</fA60>
<fA61>
<s0>A</s0>
</fA61>
<fA64 i1="01" i2="1">
<s0>Journal of clinical microbiology : (Print)</s0>
</fA64>
<fA66 i1="01">
<s0>USA</s0>
</fA66>
<fC01 i1="01" l="ENG">
<s0>We verified the analytical performance characteristics of a previously described real-time reverse transcription-PCR (RT-PCR) assay targeting the open reading frame (ORF) 1b region of the severe acute respiratory syndrome coronavirus (SARS-CoV) with RNA transcripts. We then compared it to a novel nucleocapsid gene real-time RT-PCR assay with genomic RNA. The assays differed only in the primer and probe sequences and final concentrations. A commercially available armored RNA (Ambion, Austin, Tex.) was evaluated as positive control for the ORF 1b assay. The analytical sensitivity, reproducibility, amplification efficiency, and dynamic range of the assays were similar. Both were specific for SARS-CoV as determined by testing against human CoV 229E and OC43, specimens from patients without SARS, and by BLAST searches of GenBank for primer and probe sequence homology. The armored RNA was found to be a suitable positive control for the ORF 1b assay that could be reliably recovered and amplified from a variety of clinical specimens.</s0>
</fC01>
<fC02 i1="01" i2="X">
<s0>002A05</s0>
</fC02>
<fC02 i1="02" i2="X">
<s0>002B05</s0>
</fC02>
<fC02 i1="03" i2="X">
<s0>002A05C10</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE">
<s0>Coronavirus</s0>
<s2>NW</s2>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG">
<s0>Coronavirus</s0>
<s2>NW</s2>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA">
<s0>Coronavirus</s0>
<s2>NW</s2>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE">
<s0>Temps réel</s0>
<s5>05</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG">
<s0>Real time</s0>
<s5>05</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA">
<s0>Tiempo real</s0>
<s5>05</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE">
<s0>Réaction chaîne polymérase RT</s0>
<s5>06</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG">
<s0>Reverse transcription polymerase chain reaction</s0>
<s5>06</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA">
<s0>Reacción cadena polimerasa transcripción inversa</s0>
<s5>06</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE">
<s0>Détection</s0>
<s5>07</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG">
<s0>Detection</s0>
<s5>07</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA">
<s0>Detección</s0>
<s5>07</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE">
<s0>Microbiologie</s0>
<s5>08</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG">
<s0>Microbiology</s0>
<s5>08</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA">
<s0>Microbiología</s0>
<s5>08</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE">
<s0>Syndrome respiratoire aigu sévère</s0>
<s4>CD</s4>
<s5>96</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG">
<s0>Severe acute respiratory syndrome</s0>
<s4>CD</s4>
<s5>96</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA">
<s0>Síndrome respiratorio agudo severo</s0>
<s4>CD</s4>
<s5>96</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE">
<s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG">
<s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA">
<s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE">
<s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG">
<s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA">
<s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE">
<s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG">
<s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA">
<s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE">
<s0>Appareil respiratoire pathologie</s0>
<s5>19</s5>
</fC07>
<fC07 i1="04" i2="X" l="ENG">
<s0>Respiratory disease</s0>
<s5>19</s5>
</fC07>
<fC07 i1="04" i2="X" l="SPA">
<s0>Aparato respiratorio patología</s0>
<s5>19</s5>
</fC07>
<fC07 i1="05" i2="X" l="FRE">
<s0>Poumon pathologie</s0>
<s5>20</s5>
</fC07>
<fC07 i1="05" i2="X" l="ENG">
<s0>Lung disease</s0>
<s5>20</s5>
</fC07>
<fC07 i1="05" i2="X" l="SPA">
<s0>Pulmón patología</s0>
<s5>20</s5>
</fC07>
<fC07 i1="06" i2="X" l="FRE">
<s0>Virose</s0>
<s2>NM</s2>
<s5>21</s5>
</fC07>
<fC07 i1="06" i2="X" l="ENG">
<s0>Viral disease</s0>
<s2>NM</s2>
<s5>21</s5>
</fC07>
<fC07 i1="06" i2="X" l="SPA">
<s0>Virosis</s0>
<s2>NM</s2>
<s5>21</s5>
</fC07>
<fC07 i1="07" i2="X" l="FRE">
<s0>Infection</s0>
<s2>NM</s2>
</fC07>
<fC07 i1="07" i2="X" l="ENG">
<s0>Infection</s0>
<s2>NM</s2>
</fC07>
<fC07 i1="07" i2="X" l="SPA">
<s0>Infección</s0>
<s2>NM</s2>
</fC07>
<fN21>
<s1>131</s1>
</fN21>
<fN82>
<s1>OTO</s1>
</fN82>
</pA>
</standard>
</inist>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/PascalFrancis/Curation

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000070 | SxmlIndent | more

Ou

HfdSelect -h $EXPLOR_AREA/Data/PascalFrancis/Curation/biblio.hfd -nk 000070 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    SrasV1
   |flux=    PascalFrancis
   |étape=   Curation
   |type=    RBID
   |clé=     Pascal:04-0195367
   |texte=   Preclinical evaluation of two real-time, reverse transcription-PCR assays for detection of the severe acute respiratory syndrome coronavirus
}}
Wicri

This area was generated with Dilib version V0.6.33.
Data generation: Tue Apr 28 14:49:16 2020. Site generation: Sat Mar 27 22:06:49 2021