SrasV1, PascalFrancis, Corpus, bibRecord, 000880

Evaluation of reverse transcription-PCR assays for rapid diagnosis of severe acute respiratory syndrome associated with a novel coronavirus

Identifieur interne : 000880 ( PascalFrancis/Corpus ); précédent : 000879; suivant : 000881

Evaluation of reverse transcription-PCR assays for rapid diagnosis of severe acute respiratory syndrome associated with a novel coronavirus

Auteurs : W. C. Yam ; K. H. Chan ; L. L. M. Poon ; Y. Guan ; K. Y. Yuen ; W. H. Seto ; J. S. M. Peiris

Source :

Journal of clinical microbiology : (Print) [ 0095-1137 ] ; 2003.

RBID : Pascal:04-0313747

Descripteurs français

Pascal (Inist)
- Coronavirus, Réaction chaîne polymérase RT, Diagnostic, Microbiologie, Syndrome respiratoire aigu sévère.

English descriptors

KwdEn :
- Coronavirus, Diagnosis, Microbiology, Reverse transcription polymerase chain reaction, Severe acute respiratory syndrome.

Abstract

The reverse transcription (RT)-PCR protocols of two World Health Organization (WHO) severe acute respiratory syndrome (SARS) network laboratories (WHO SARS network laboratories at The University of Hong Kong [WHO-HKU] and at the Bernhard-Nocht Institute in Hamburg, Germany [WHO-Hamburg]) were evaluated for rapid diagnosis of a novel coronavirus (CoV) associated with SARS in Hong Kong. A total of 303 clinical specimens were collected from 163 patients suspected to have SARS. The end point of both WHO-HKU and WHO-Hamburg RT-PCR assays was determined to be 0.1 50% tissue culture infective dose. Using seroconversion to CoV as the "gold standard" for SARS CoV diagnosis, WHO-HKU and WHO-Hamburg RT-PCR assays exhibited diagnostic sensitivities of 61 and 68% (nasopharyngeal aspirate specimens), 65 and 72% (throat swab specimens), 50 and 54% (urine specimens), and 58 and 63% (stool specimens), respectively, with an overall specificity of 100%. For patients confirmed to have SARS CoV and from whom two or more respiratory specimens were collected, testing the second specimen increased the sensitivity from 64 and 71% to 75 and 79% for the WHO-HKU and WHO-Hamburg RT-PCR assays, respectively. Testing more than one respiratory specimen will maximize the sensitivity of PCR assays for SARS CoV.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

A01	`01`	`1`		`@0 0095-1137`
A02	`01`			`@0 JCMIDW`
A03		`1`		`@0 J. clin. microbiol. : (Print)`
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A11	`01`	`1`		`@1 YAM (W. C.)`
A11	`02`	`1`		`@1 CHAN (K. H.)`
A11	`03`	`1`		`@1 POON (L. L. M.)`
A11	`04`	`1`		`@1 GUAN (Y.)`
A11	`05`	`1`		`@1 YUEN (K. Y.)`
A11	`06`	`1`		`@1 SETO (W. H.)`
A11	`07`	`1`		`@1 PEIRIS (J. S. M.)`
A14	`01`			`@1 Department of Microbiology, Queen Mary Hospital, The University of Hong Kong @3 HKG @Z 1 aut. @Z 2 aut. @Z 3 aut. @Z 4 aut. @Z 5 aut. @Z 6 aut. @Z 7 aut.`
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A21				`@1 2003`
A23	`01`			`@0 ENG`
A43	`01`			`@1 INIST @2 17088 @5 354000113348600040`
A44				`@0 0000 @1 © 2004 INIST-CNRS. All rights reserved.`
A45				`@0 13 ref.`
A47	`01`	`1`		`@0 04-0313747`
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A61				`@0 A`
A64	`01`	`1`		`@0 Journal of clinical microbiology : (Print)`
A66	`01`			`@0 USA`
C01	`01`		`ENG`	@0 The reverse transcription (RT)-PCR protocols of two World Health Organization (WHO) severe acute respiratory syndrome (SARS) network laboratories (WHO SARS network laboratories at The University of Hong Kong [WHO-HKU] and at the Bernhard-Nocht Institute in Hamburg, Germany [WHO-Hamburg]) were evaluated for rapid diagnosis of a novel coronavirus (CoV) associated with SARS in Hong Kong. A total of 303 clinical specimens were collected from 163 patients suspected to have SARS. The end point of both WHO-HKU and WHO-Hamburg RT-PCR assays was determined to be 0.1 50% tissue culture infective dose. Using seroconversion to CoV as the "gold standard" for SARS CoV diagnosis, WHO-HKU and WHO-Hamburg RT-PCR assays exhibited diagnostic sensitivities of 61 and 68% (nasopharyngeal aspirate specimens), 65 and 72% (throat swab specimens), 50 and 54% (urine specimens), and 58 and 63% (stool specimens), respectively, with an overall specificity of 100%. For patients confirmed to have SARS CoV and from whom two or more respiratory specimens were collected, testing the second specimen increased the sensitivity from 64 and 71% to 75 and 79% for the WHO-HKU and WHO-Hamburg RT-PCR assays, respectively. Testing more than one respiratory specimen will maximize the sensitivity of PCR assays for SARS CoV.
C02	`01`	`X`		`@0 002A05C10`
C02	`02`	`X`		`@0 002B05`
C03	`01`	`X`	`FRE`	`@0 Coronavirus @2 NW @5 01`
C03	`01`	`X`	`ENG`	`@0 Coronavirus @2 NW @5 01`
C03	`01`	`X`	`SPA`	`@0 Coronavirus @2 NW @5 01`
C03	`02`	`X`	`FRE`	`@0 Réaction chaîne polymérase RT @5 05`
C03	`02`	`X`	`ENG`	`@0 Reverse transcription polymerase chain reaction @5 05`
C03	`02`	`X`	`SPA`	`@0 Reacción cadena polimerasa transcripción inversa @5 05`
C03	`03`	`X`	`FRE`	`@0 Diagnostic @5 06`
C03	`03`	`X`	`ENG`	`@0 Diagnosis @5 06`
C03	`03`	`X`	`SPA`	`@0 Diagnóstico @5 06`
C03	`04`	`X`	`FRE`	`@0 Microbiologie @5 07`
C03	`04`	`X`	`ENG`	`@0 Microbiology @5 07`
C03	`04`	`X`	`SPA`	`@0 Microbiología @5 07`
C03	`05`	`X`	`FRE`	`@0 Syndrome respiratoire aigu sévère @4 CD @5 96`
C03	`05`	`X`	`ENG`	`@0 Severe acute respiratory syndrome @4 CD @5 96`
C03	`05`	`X`	`SPA`	`@0 Síndrome respiratorio agudo severo @4 CD @5 96`
C07	`01`	`X`	`FRE`	`@0 Coronaviridae @2 NW`
C07	`01`	`X`	`ENG`	`@0 Coronaviridae @2 NW`
C07	`01`	`X`	`SPA`	`@0 Coronaviridae @2 NW`
C07	`02`	`X`	`FRE`	`@0 Nidovirales @2 NW`
C07	`02`	`X`	`ENG`	`@0 Nidovirales @2 NW`
C07	`02`	`X`	`SPA`	`@0 Nidovirales @2 NW`
C07	`03`	`X`	`FRE`	`@0 Virus @2 NW`
C07	`03`	`X`	`ENG`	`@0 Virus @2 NW`
C07	`03`	`X`	`SPA`	`@0 Virus @2 NW`
C07	`04`	`X`	`FRE`	`@0 Appareil respiratoire pathologie @5 19`
C07	`04`	`X`	`ENG`	`@0 Respiratory disease @5 19`
C07	`04`	`X`	`SPA`	`@0 Aparato respiratorio patología @5 19`
C07	`05`	`X`	`FRE`	`@0 Poumon pathologie @5 20`
C07	`05`	`X`	`ENG`	`@0 Lung disease @5 20`
C07	`05`	`X`	`SPA`	`@0 Pulmón patología @5 20`
C07	`06`	`X`	`FRE`	`@0 Virose @2 NM @5 21`
C07	`06`	`X`	`ENG`	`@0 Viral disease @2 NM @5 21`
C07	`06`	`X`	`SPA`	`@0 Virosis @2 NM @5 21`
C07	`07`	`X`	`FRE`	`@0 Infection @2 NM`
C07	`07`	`X`	`ENG`	`@0 Infection @2 NM`
C07	`07`	`X`	`SPA`	`@0 Infección @2 NM`
N21				`@1 187`
N44	`01`			`@1 OTO`
N82				`@1 OTO`

Format Inist (serveur)

NO :	PASCAL 04-0313747 INIST
ET :	Evaluation of reverse transcription-PCR assays for rapid diagnosis of severe acute respiratory syndrome associated with a novel coronavirus
AU :	YAM (W. C.); CHAN (K. H.); POON (L. L. M.); GUAN (Y.); YUEN (K. Y.); SETO (W. H.); PEIRIS (J. S. M.)
AF :	Department of Microbiology, Queen Mary Hospital, The University of Hong Kong/Hong-Kong (1 aut., 2 aut., 3 aut., 4 aut., 5 aut., 6 aut., 7 aut.)
DT :	Publication en série; Niveau analytique
SO :	Journal of clinical microbiology : (Print); ISSN 0095-1137; Coden JCMIDW; Etats-Unis; Da. 2003; Vol. 41; No. 10; Pp. 4521-4524; Bibl. 13 ref.
LA :	Anglais
EA :	The reverse transcription (RT)-PCR protocols of two World Health Organization (WHO) severe acute respiratory syndrome (SARS) network laboratories (WHO SARS network laboratories at The University of Hong Kong [WHO-HKU] and at the Bernhard-Nocht Institute in Hamburg, Germany [WHO-Hamburg]) were evaluated for rapid diagnosis of a novel coronavirus (CoV) associated with SARS in Hong Kong. A total of 303 clinical specimens were collected from 163 patients suspected to have SARS. The end point of both WHO-HKU and WHO-Hamburg RT-PCR assays was determined to be 0.1 50% tissue culture infective dose. Using seroconversion to CoV as the "gold standard" for SARS CoV diagnosis, WHO-HKU and WHO-Hamburg RT-PCR assays exhibited diagnostic sensitivities of 61 and 68% (nasopharyngeal aspirate specimens), 65 and 72% (throat swab specimens), 50 and 54% (urine specimens), and 58 and 63% (stool specimens), respectively, with an overall specificity of 100%. For patients confirmed to have SARS CoV and from whom two or more respiratory specimens were collected, testing the second specimen increased the sensitivity from 64 and 71% to 75 and 79% for the WHO-HKU and WHO-Hamburg RT-PCR assays, respectively. Testing more than one respiratory specimen will maximize the sensitivity of PCR assays for SARS CoV.
CC :	002A05C10; 002B05
FD :	Coronavirus; Réaction chaîne polymérase RT; Diagnostic; Microbiologie; Syndrome respiratoire aigu sévère
FG :	Coronaviridae; Nidovirales; Virus; Appareil respiratoire pathologie; Poumon pathologie; Virose; Infection
ED :	Coronavirus; Reverse transcription polymerase chain reaction; Diagnosis; Microbiology; Severe acute respiratory syndrome
EG :	Coronaviridae; Nidovirales; Virus; Respiratory disease; Lung disease; Viral disease; Infection
SD :	Coronavirus; Reacción cadena polimerasa transcripción inversa; Diagnóstico; Microbiología; Síndrome respiratorio agudo severo
LO :	INIST-17088.354000113348600040
ID :	04-0313747

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Pascal:04-0313747

Le document en format XML

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<front><div type="abstract" xml:lang="en">The reverse transcription (RT)-PCR protocols of two World Health Organization (WHO) severe acute respiratory syndrome (SARS) network laboratories (WHO SARS network laboratories at The University of Hong Kong [WHO-HKU] and at the Bernhard-Nocht Institute in Hamburg, Germany [WHO-Hamburg]) were evaluated for rapid diagnosis of a novel coronavirus (CoV) associated with SARS in Hong Kong. A total of 303 clinical specimens were collected from 163 patients suspected to have SARS. The end point of both WHO-HKU and WHO-Hamburg RT-PCR assays was determined to be 0.1 50% tissue culture infective dose. Using seroconversion to CoV as the "gold standard" for SARS CoV diagnosis, WHO-HKU and WHO-Hamburg RT-PCR assays exhibited diagnostic sensitivities of 61 and 68% (nasopharyngeal aspirate specimens), 65 and 72% (throat swab specimens), 50 and 54% (urine specimens), and 58 and 63% (stool specimens), respectively, with an overall specificity of 100%. For patients confirmed to have SARS CoV and from whom two or more respiratory specimens were collected, testing the second specimen increased the sensitivity from 64 and 71% to 75 and 79% for the WHO-HKU and WHO-Hamburg RT-PCR assays, respectively. Testing more than one respiratory specimen will maximize the sensitivity of PCR assays for SARS CoV.</div>
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<server><NO>PASCAL 04-0313747 INIST</NO>
<ET>Evaluation of reverse transcription-PCR assays for rapid diagnosis of severe acute respiratory syndrome associated with a novel coronavirus</ET>
<AU>YAM (W. C.); CHAN (K. H.); POON (L. L. M.); GUAN (Y.); YUEN (K. Y.); SETO (W. H.); PEIRIS (J. S. M.)</AU>
<AF>Department of Microbiology, Queen Mary Hospital, The University of Hong Kong/Hong-Kong (1 aut., 2 aut., 3 aut., 4 aut., 5 aut., 6 aut., 7 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Journal of clinical microbiology : (Print); ISSN 0095-1137; Coden JCMIDW; Etats-Unis; Da. 2003; Vol. 41; No. 10; Pp. 4521-4524; Bibl. 13 ref.</SO>
<LA>Anglais</LA>
<EA>The reverse transcription (RT)-PCR protocols of two World Health Organization (WHO) severe acute respiratory syndrome (SARS) network laboratories (WHO SARS network laboratories at The University of Hong Kong [WHO-HKU] and at the Bernhard-Nocht Institute in Hamburg, Germany [WHO-Hamburg]) were evaluated for rapid diagnosis of a novel coronavirus (CoV) associated with SARS in Hong Kong. A total of 303 clinical specimens were collected from 163 patients suspected to have SARS. The end point of both WHO-HKU and WHO-Hamburg RT-PCR assays was determined to be 0.1 50% tissue culture infective dose. Using seroconversion to CoV as the "gold standard" for SARS CoV diagnosis, WHO-HKU and WHO-Hamburg RT-PCR assays exhibited diagnostic sensitivities of 61 and 68% (nasopharyngeal aspirate specimens), 65 and 72% (throat swab specimens), 50 and 54% (urine specimens), and 58 and 63% (stool specimens), respectively, with an overall specificity of 100%. For patients confirmed to have SARS CoV and from whom two or more respiratory specimens were collected, testing the second specimen increased the sensitivity from 64 and 71% to 75 and 79% for the WHO-HKU and WHO-Hamburg RT-PCR assays, respectively. Testing more than one respiratory specimen will maximize the sensitivity of PCR assays for SARS CoV.</EA>
<CC>002A05C10; 002B05</CC>
<FD>Coronavirus; Réaction chaîne polymérase RT; Diagnostic; Microbiologie; Syndrome respiratoire aigu sévère</FD>
<FG>Coronaviridae; Nidovirales; Virus; Appareil respiratoire pathologie; Poumon pathologie; Virose; Infection</FG>
<ED>Coronavirus; Reverse transcription polymerase chain reaction; Diagnosis; Microbiology; Severe acute respiratory syndrome</ED>
<EG>Coronaviridae; Nidovirales; Virus; Respiratory disease; Lung disease; Viral disease; Infection</EG>
<SD>Coronavirus; Reacción cadena polimerasa transcripción inversa; Diagnóstico; Microbiología; Síndrome respiratorio agudo severo</SD>
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Evaluation of reverse transcription-PCR assays for rapid diagnosis of severe acute respiratory syndrome associated with a novel coronavirus

Evaluation of reverse transcription-PCR assays for rapid diagnosis of severe acute respiratory syndrome associated with a novel coronavirus

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