Generation of synthetic severe acute respiratory syndrome coronavirus pseudoparticles: Implications for assembly and vaccine production
Identifieur interne : 000760 ( PascalFrancis/Corpus ); précédent : 000759; suivant : 000761Generation of synthetic severe acute respiratory syndrome coronavirus pseudoparticles: Implications for assembly and vaccine production
Auteurs : YUE HUANG ; Zhi-Yong Yang ; Wing-Pui Kong ; Gary J. NabelSource :
- Journal of virology [ 0022-538X ] ; 2004.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
The recently emerged severe acute respiratory syndrome coronavirus (SARS-CoV) contains four structural genes, two replicase-transcriptase open reading frames, and more than five potential genes of unknown function. Despite this relative simplicity, the molecular regulation of SARS-CoV replication and assembly is not understood. Here, we report that two viral genes, encoding the SARS-CoV membrane (M) and nucleocapsid (N) proteins, are necessary and sufficient for formation of virus-like particles. Expression vectors encoding these two proteins were synthesized by using preferred human codons. When M and N expression plasmids were cotransfected into human 293 renal epithelial cells, pseudoparticles formed readily. The addition of a third gene, encoding the spike (S) glycoprotein, facilitated budding of particles that contained a corona-like halo resembling SARS-CoV when examined by transmission electron microscopy, with a buoyant density characteristic of coronaviruses. Specific biochemical interactions of these proteins were also shown in vitro. The S, M, and N proteins of the SARS-CoV are, therefore, necessary and sufficient for pseudovirus assembly. These findings advance the understanding of the morphogenesis of SARS-CoV and enable the generation of safe, conformational mimetics of the SARS virus that may facilitate the development of vaccines and antiviral drugs.
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Format Inist (serveur)
NO : | PASCAL 05-0000571 INIST |
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ET : | Generation of synthetic severe acute respiratory syndrome coronavirus pseudoparticles: Implications for assembly and vaccine production |
AU : | YUE HUANG; YANG (Zhi-Yong); KONG (Wing-Pui); NABEL (Gary J.) |
AF : | Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health/Bethesda, Maryland/Etats-Unis (1 aut., 2 aut., 3 aut., 4 aut.) |
DT : | Publication en série; Niveau analytique |
SO : | Journal of virology; ISSN 0022-538X; Etats-Unis; Da. 2004; Vol. 78; No. 22; Pp. 12557-12565; Bibl. 35 ref. |
LA : | Anglais |
EA : | The recently emerged severe acute respiratory syndrome coronavirus (SARS-CoV) contains four structural genes, two replicase-transcriptase open reading frames, and more than five potential genes of unknown function. Despite this relative simplicity, the molecular regulation of SARS-CoV replication and assembly is not understood. Here, we report that two viral genes, encoding the SARS-CoV membrane (M) and nucleocapsid (N) proteins, are necessary and sufficient for formation of virus-like particles. Expression vectors encoding these two proteins were synthesized by using preferred human codons. When M and N expression plasmids were cotransfected into human 293 renal epithelial cells, pseudoparticles formed readily. The addition of a third gene, encoding the spike (S) glycoprotein, facilitated budding of particles that contained a corona-like halo resembling SARS-CoV when examined by transmission electron microscopy, with a buoyant density characteristic of coronaviruses. Specific biochemical interactions of these proteins were also shown in vitro. The S, M, and N proteins of the SARS-CoV are, therefore, necessary and sufficient for pseudovirus assembly. These findings advance the understanding of the morphogenesis of SARS-CoV and enable the generation of safe, conformational mimetics of the SARS virus that may facilitate the development of vaccines and antiviral drugs. |
CC : | 002A05C10; 002A05C07 |
FD : | Coronavirus; Grave; Malade état grave; Aigu; Voie respiratoire; Vaccin; Prévention; Immunoprophylaxie; Microbiologie; Syndrome respiratoire aigu sévère; Virologie; Forme grave |
FG : | Coronaviridae; Nidovirales; Virus; Virose; Infection; Appareil respiratoire pathologie; Poumon pathologie; Appareil respiratoire |
ED : | Coronavirus; Severe; Critically ill; Acute; Respiratory tract; Vaccine; Prevention; Immunoprophylaxis; Microbiology; Severe acute respiratory syndrome; Virology |
EG : | Coronaviridae; Nidovirales; Virus; Viral disease; Infection; Respiratory disease; Lung disease; Respiratory system |
SD : | Coronavirus; Grave; Enfermo estado grave; Agudo; Vía respiratoria; Vacuna; Prevención; Inmunoprofilaxia; Microbiología; Síndrome respiratorio agudo severo; Virología |
LO : | INIST-13592.354000122578960480 |
ID : | 05-0000571 |
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<front><div type="abstract" xml:lang="en">The recently emerged severe acute respiratory syndrome coronavirus (SARS-CoV) contains four structural genes, two replicase-transcriptase open reading frames, and more than five potential genes of unknown function. Despite this relative simplicity, the molecular regulation of SARS-CoV replication and assembly is not understood. Here, we report that two viral genes, encoding the SARS-CoV membrane (M) and nucleocapsid (N) proteins, are necessary and sufficient for formation of virus-like particles. Expression vectors encoding these two proteins were synthesized by using preferred human codons. When M and N expression plasmids were cotransfected into human 293 renal epithelial cells, pseudoparticles formed readily. The addition of a third gene, encoding the spike (S) glycoprotein, facilitated budding of particles that contained a corona-like halo resembling SARS-CoV when examined by transmission electron microscopy, with a buoyant density characteristic of coronaviruses. Specific biochemical interactions of these proteins were also shown in vitro. The S, M, and N proteins of the SARS-CoV are, therefore, necessary and sufficient for pseudovirus assembly. These findings advance the understanding of the morphogenesis of SARS-CoV and enable the generation of safe, conformational mimetics of the SARS virus that may facilitate the development of vaccines and antiviral drugs.</div>
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<server><NO>PASCAL 05-0000571 INIST</NO>
<ET>Generation of synthetic severe acute respiratory syndrome coronavirus pseudoparticles: Implications for assembly and vaccine production</ET>
<AU>YUE HUANG; YANG (Zhi-Yong); KONG (Wing-Pui); NABEL (Gary J.)</AU>
<AF>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health/Bethesda, Maryland/Etats-Unis (1 aut., 2 aut., 3 aut., 4 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Journal of virology; ISSN 0022-538X; Etats-Unis; Da. 2004; Vol. 78; No. 22; Pp. 12557-12565; Bibl. 35 ref.</SO>
<LA>Anglais</LA>
<EA>The recently emerged severe acute respiratory syndrome coronavirus (SARS-CoV) contains four structural genes, two replicase-transcriptase open reading frames, and more than five potential genes of unknown function. Despite this relative simplicity, the molecular regulation of SARS-CoV replication and assembly is not understood. Here, we report that two viral genes, encoding the SARS-CoV membrane (M) and nucleocapsid (N) proteins, are necessary and sufficient for formation of virus-like particles. Expression vectors encoding these two proteins were synthesized by using preferred human codons. When M and N expression plasmids were cotransfected into human 293 renal epithelial cells, pseudoparticles formed readily. The addition of a third gene, encoding the spike (S) glycoprotein, facilitated budding of particles that contained a corona-like halo resembling SARS-CoV when examined by transmission electron microscopy, with a buoyant density characteristic of coronaviruses. Specific biochemical interactions of these proteins were also shown in vitro. The S, M, and N proteins of the SARS-CoV are, therefore, necessary and sufficient for pseudovirus assembly. These findings advance the understanding of the morphogenesis of SARS-CoV and enable the generation of safe, conformational mimetics of the SARS virus that may facilitate the development of vaccines and antiviral drugs.</EA>
<CC>002A05C10; 002A05C07</CC>
<FD>Coronavirus; Grave; Malade état grave; Aigu; Voie respiratoire; Vaccin; Prévention; Immunoprophylaxie; Microbiologie; Syndrome respiratoire aigu sévère; Virologie; Forme grave</FD>
<FG>Coronaviridae; Nidovirales; Virus; Virose; Infection; Appareil respiratoire pathologie; Poumon pathologie; Appareil respiratoire</FG>
<ED>Coronavirus; Severe; Critically ill; Acute; Respiratory tract; Vaccine; Prevention; Immunoprophylaxis; Microbiology; Severe acute respiratory syndrome; Virology</ED>
<EG>Coronaviridae; Nidovirales; Virus; Viral disease; Infection; Respiratory disease; Lung disease; Respiratory system</EG>
<SD>Coronavirus; Grave; Enfermo estado grave; Agudo; Vía respiratoria; Vacuna; Prevención; Inmunoprofilaxia; Microbiología; Síndrome respiratorio agudo severo; Virología</SD>
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