Identification of two neutralizing regions on the severe acute respiratory syndrome coronavirus Spike glycoprotein produced from the mammalian expression system
Identifieur interne : 000720 ( PascalFrancis/Corpus ); précédent : 000719; suivant : 000721Identification of two neutralizing regions on the severe acute respiratory syndrome coronavirus Spike glycoprotein produced from the mammalian expression system
Auteurs : SHIXIA WANG ; Te-Hui W. Chou ; Pavlo V. Sakhatskyy ; SONG HUANG ; John M. Lawrence ; HONG CAO ; XIAOYUN HUANG ; SHAN LUSource :
- Journal of virology [ 0022-538X ] ; 2005.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
The Spike (S) protein of the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) plays important roles in viral pathogenesis and potentially in the development of an effective vaccine against this virulent infectious disease. In this study, the codon-optimized S gene of SARS-CoV was synthesized to construct DNA vaccine plasmids expressing either the full-length or segments of the S protein. High titer S-specific immunoglobulin G antibody responses were elicited in rabbits immunized with DNA against various segments of the S protein. Two neutralizing domains were identified on the S protein, one at the N terminus (Ser12-Thr535) and the other near the C terminus (Arg797-Ile1192).
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Format Inist (serveur)
NO : | PASCAL 05-0133038 INIST |
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ET : | Identification of two neutralizing regions on the severe acute respiratory syndrome coronavirus Spike glycoprotein produced from the mammalian expression system |
AU : | SHIXIA WANG; CHOU (Te-Hui W.); SAKHATSKYY (Pavlo V.); SONG HUANG; LAWRENCE (John M.); HONG CAO; XIAOYUN HUANG; SHAN LU |
AF : | Laboratory of Nucleic Acid Vaccines, Department of Medicine, University of Massachusetts Medical School/Worcester, Massachusetts/Etats-Unis (1 aut., 2 aut., 3 aut., 4 aut., 5 aut., 6 aut., 7 aut., 8 aut.) |
DT : | Publication en série; Niveau analytique |
SO : | Journal of virology; ISSN 0022-538X; Etats-Unis; Da. 2005; Vol. 79; No. 3; Pp. 1906-1910; Bibl. 29 ref. |
LA : | Anglais |
EA : | The Spike (S) protein of the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) plays important roles in viral pathogenesis and potentially in the development of an effective vaccine against this virulent infectious disease. In this study, the codon-optimized S gene of SARS-CoV was synthesized to construct DNA vaccine plasmids expressing either the full-length or segments of the S protein. High titer S-specific immunoglobulin G antibody responses were elicited in rabbits immunized with DNA against various segments of the S protein. Two neutralizing domains were identified on the S protein, one at the N terminus (Ser12-Thr535) and the other near the C terminus (Arg797-Ile1192). |
CC : | 002A05C10 |
FD : | Coronavirus; Mammalia; Identification; Glycoprotéine; Microbiologie; Virologie; Syndrome respiratoire aigu sévère |
FG : | Coronaviridae; Nidovirales; Virus; Vertebrata; Appareil respiratoire pathologie; Virose; Infection; Poumon pathologie |
ED : | Coronavirus; Mammalia; Identification; Glycoprotein; Microbiology; Virology; Severe acute respiratory syndrome |
EG : | Coronaviridae; Nidovirales; Virus; Vertebrata; Respiratory disease; Viral disease; Infection; Lung disease |
SD : | Coronavirus; Mammalia; Identificación; Glicoproteína; Microbiología; Virología; Síndrome respiratorio agudo severo |
LO : | INIST-13592.354000127060900550 |
ID : | 05-0133038 |
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Pascal:05-0133038Le document en format XML
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<front><div type="abstract" xml:lang="en">The Spike (S) protein of the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) plays important roles in viral pathogenesis and potentially in the development of an effective vaccine against this virulent infectious disease. In this study, the codon-optimized S gene of SARS-CoV was synthesized to construct DNA vaccine plasmids expressing either the full-length or segments of the S protein. High titer S-specific immunoglobulin G antibody responses were elicited in rabbits immunized with DNA against various segments of the S protein. Two neutralizing domains were identified on the S protein, one at the N terminus (Ser12-Thr535) and the other near the C terminus (Arg797-Ile1192).</div>
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</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
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<s2>NW</s2>
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<fC07 i1="03" i2="X" l="SPA"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Vertebrata</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Vertebrata</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Vertebrata</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Appareil respiratoire pathologie</s0>
<s5>13</s5>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Respiratory disease</s0>
<s5>13</s5>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Aparato respiratorio patología</s0>
<s5>13</s5>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Virose</s0>
</fC07>
<fC07 i1="06" i2="X" l="ENG"><s0>Viral disease</s0>
</fC07>
<fC07 i1="06" i2="X" l="SPA"><s0>Virosis</s0>
</fC07>
<fC07 i1="07" i2="X" l="FRE"><s0>Infection</s0>
</fC07>
<fC07 i1="07" i2="X" l="ENG"><s0>Infection</s0>
</fC07>
<fC07 i1="07" i2="X" l="SPA"><s0>Infección</s0>
</fC07>
<fC07 i1="08" i2="X" l="FRE"><s0>Poumon pathologie</s0>
<s5>16</s5>
</fC07>
<fC07 i1="08" i2="X" l="ENG"><s0>Lung disease</s0>
<s5>16</s5>
</fC07>
<fC07 i1="08" i2="X" l="SPA"><s0>Pulmón patología</s0>
<s5>16</s5>
</fC07>
<fN21><s1>087</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
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<server><NO>PASCAL 05-0133038 INIST</NO>
<ET>Identification of two neutralizing regions on the severe acute respiratory syndrome coronavirus Spike glycoprotein produced from the mammalian expression system</ET>
<AU>SHIXIA WANG; CHOU (Te-Hui W.); SAKHATSKYY (Pavlo V.); SONG HUANG; LAWRENCE (John M.); HONG CAO; XIAOYUN HUANG; SHAN LU</AU>
<AF>Laboratory of Nucleic Acid Vaccines, Department of Medicine, University of Massachusetts Medical School/Worcester, Massachusetts/Etats-Unis (1 aut., 2 aut., 3 aut., 4 aut., 5 aut., 6 aut., 7 aut., 8 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Journal of virology; ISSN 0022-538X; Etats-Unis; Da. 2005; Vol. 79; No. 3; Pp. 1906-1910; Bibl. 29 ref.</SO>
<LA>Anglais</LA>
<EA>The Spike (S) protein of the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) plays important roles in viral pathogenesis and potentially in the development of an effective vaccine against this virulent infectious disease. In this study, the codon-optimized S gene of SARS-CoV was synthesized to construct DNA vaccine plasmids expressing either the full-length or segments of the S protein. High titer S-specific immunoglobulin G antibody responses were elicited in rabbits immunized with DNA against various segments of the S protein. Two neutralizing domains were identified on the S protein, one at the N terminus (Ser12-Thr535) and the other near the C terminus (Arg797-Ile1192).</EA>
<CC>002A05C10</CC>
<FD>Coronavirus; Mammalia; Identification; Glycoprotéine; Microbiologie; Virologie; Syndrome respiratoire aigu sévère</FD>
<FG>Coronaviridae; Nidovirales; Virus; Vertebrata; Appareil respiratoire pathologie; Virose; Infection; Poumon pathologie</FG>
<ED>Coronavirus; Mammalia; Identification; Glycoprotein; Microbiology; Virology; Severe acute respiratory syndrome</ED>
<EG>Coronaviridae; Nidovirales; Virus; Vertebrata; Respiratory disease; Viral disease; Infection; Lung disease</EG>
<SD>Coronavirus; Mammalia; Identificación; Glicoproteína; Microbiología; Virología; Síndrome respiratorio agudo severo</SD>
<LO>INIST-13592.354000127060900550</LO>
<ID>05-0133038</ID>
</server>
</inist>
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