SrasV1, PascalFrancis, Corpus, bibRecord, 000699

Clinical evaluation of real-time PCR assays for rapid diagnosis of SARS coronavims during outbreak and post-epidemic periods

Identifieur interne : 000699 ( PascalFrancis/Corpus ); précédent : 000698; suivant : 000700

Clinical evaluation of real-time PCR assays for rapid diagnosis of SARS coronavims during outbreak and post-epidemic periods

Auteurs : W. C. Yam ; K. H. Chan ; K. H. Chow ; L. L. M. Poon ; H. Y. Lam ; K. Y. Yuen ; W. H. Seto ; J. S. M. Peiris

Source :

Journal of clinical virology [ 1386-6532 ] ; 2005.

RBID : Pascal:05-0205858

Descripteurs français

Pascal (Inist)
- Virus syndrome respiratoire aigu sévère, Temps réel, Réaction chaîne polymérase, Diagnostic, Epidémie, Microbiologie, Virologie, Syndrome respiratoire aigu sévère.

English descriptors

KwdEn :
- Diagnosis, Epidemic, Microbiology, Polymerase chain reaction, Real time, Severe acute respiratory syndrome, Severe acute respiratory syndrome virus, Virology.

Abstract

Background: The protocols of WHO network laboratories facilitated development of rapid diagnosis for SARS coronavirus (CoV) using reverse transcription (RT)-PCR assays. However, several reports have shown that conventional and real-time PCR assays were very specific for SARS CoV but lack sensitivity depending on the assay, specimen, and time course of disease. Objective: To evaluate an automatic nucleic acid extraction system and two standardized real-time PCR assays for rapid diagnosis of SARS CoV during outbreak and post-epidemic periods in Hong Kong. Study design: Specimens from clinically suspected SARS patients collected during outbreak and post-epidemic periods were tested by an automatic nucleic acid extraction system followed by our first generation conventional RT-PCR and two standardized real-time PCR assays (Artus GmbH, Germany and Roche Diagnostics, Germany). Paired serum samples were assayed for increasing titer against SARS CoV. Results: In the SARS epidemic, Artus and Roche PCR assays exhibited sensitivities of 87% and 85% for respiratory specimens (n = 64), 91% and 88% for stool (n=44), and 82% for urine (n = 29). A specificity of 100% was exhibited by both PCR assays except Artus attained only a 92% specificity for stool. For post-epidemic period, no SARS CoV was identified among 56 respiratory specimens by all PCR assays. Inhibitors to PCR assays were detected at an average rate of 7-8% among 202 clinical specimens. Conclusion: This study highlights the high throughput and performance of automatic RNA extraction in coordination with standardized real-time PCR assays suitable for large-scale routine diagnosis in case of future SARS epidemic. As no SARS CoV was detected among specimens collected during post-epidemic period, the positive predictive value of real-time PCR assays for detection of SARS CoV during low epidemic requires further evaluation.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

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A03		`1`		`@0 J. clin. virol.`
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A11	`01`	`1`		`@1 YAM (W. C.)`
A11	`02`	`1`		`@1 CHAN (K. H.)`
A11	`03`	`1`		`@1 CHOW (K. H.)`
A11	`04`	`1`		`@1 POON (L. L. M.)`
A11	`05`	`1`		`@1 LAM (H. Y.)`
A11	`06`	`1`		`@1 YUEN (K. Y.)`
A11	`07`	`1`		`@1 SETO (W. H.)`
A11	`08`	`1`		`@1 PEIRIS (J. S. M.)`
A14	`01`			`@1 Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital Compound @2 Pokfulam @3 HKG @Z 1 aut. @Z 2 aut. @Z 3 aut. @Z 4 aut. @Z 5 aut. @Z 6 aut. @Z 7 aut. @Z 8 aut.`
A20				`@1 19-24`
A21				`@1 2005`
A23	`01`			`@0 ENG`
A43	`01`			`@1 INIST @2 26272 @5 354000125656290040`
A44				`@0 0000 @1 © 2005 INIST-CNRS. All rights reserved.`
A45				`@0 15 ref.`
A47	`01`	`1`		`@0 05-0205858`
A60				`@1 P`
A61				`@0 A`
A64	`01`	`1`		`@0 Journal of clinical virology`
A66	`01`			`@0 NLD`
C01	`01`		`ENG`	@0 Background: The protocols of WHO network laboratories facilitated development of rapid diagnosis for SARS coronavirus (CoV) using reverse transcription (RT)-PCR assays. However, several reports have shown that conventional and real-time PCR assays were very specific for SARS CoV but lack sensitivity depending on the assay, specimen, and time course of disease. Objective: To evaluate an automatic nucleic acid extraction system and two standardized real-time PCR assays for rapid diagnosis of SARS CoV during outbreak and post-epidemic periods in Hong Kong. Study design: Specimens from clinically suspected SARS patients collected during outbreak and post-epidemic periods were tested by an automatic nucleic acid extraction system followed by our first generation conventional RT-PCR and two standardized real-time PCR assays (Artus GmbH, Germany and Roche Diagnostics, Germany). Paired serum samples were assayed for increasing titer against SARS CoV. Results: In the SARS epidemic, Artus and Roche PCR assays exhibited sensitivities of 87% and 85% for respiratory specimens (n = 64), 91% and 88% for stool (n=44), and 82% for urine (n = 29). A specificity of 100% was exhibited by both PCR assays except Artus attained only a 92% specificity for stool. For post-epidemic period, no SARS CoV was identified among 56 respiratory specimens by all PCR assays. Inhibitors to PCR assays were detected at an average rate of 7-8% among 202 clinical specimens. Conclusion: This study highlights the high throughput and performance of automatic RNA extraction in coordination with standardized real-time PCR assays suitable for large-scale routine diagnosis in case of future SARS epidemic. As no SARS CoV was detected among specimens collected during post-epidemic period, the positive predictive value of real-time PCR assays for detection of SARS CoV during low epidemic requires further evaluation.
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C02	`02`	`X`		`@0 002B05C02J`
C03	`01`	`X`	`FRE`	`@0 Virus syndrome respiratoire aigu sévère @2 NW @5 01`
C03	`01`	`X`	`ENG`	`@0 Severe acute respiratory syndrome virus @2 NW @5 01`
C03	`01`	`X`	`SPA`	`@0 Severe acute respiratory syndrome virus @2 NW @5 01`
C03	`02`	`X`	`FRE`	`@0 Temps réel @5 05`
C03	`02`	`X`	`ENG`	`@0 Real time @5 05`
C03	`02`	`X`	`SPA`	`@0 Tiempo real @5 05`
C03	`03`	`X`	`FRE`	`@0 Réaction chaîne polymérase @5 06`
C03	`03`	`X`	`ENG`	`@0 Polymerase chain reaction @5 06`
C03	`03`	`X`	`SPA`	`@0 Reacción cadena polimerasa @5 06`
C03	`04`	`X`	`FRE`	`@0 Diagnostic @5 07`
C03	`04`	`X`	`ENG`	`@0 Diagnosis @5 07`
C03	`04`	`X`	`SPA`	`@0 Diagnóstico @5 07`
C03	`05`	`X`	`FRE`	`@0 Epidémie @5 08`
C03	`05`	`X`	`ENG`	`@0 Epidemic @5 08`
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C03	`06`	`X`	`FRE`	`@0 Microbiologie @5 09`
C03	`06`	`X`	`ENG`	`@0 Microbiology @5 09`
C03	`06`	`X`	`SPA`	`@0 Microbiología @5 09`
C03	`07`	`X`	`FRE`	`@0 Virologie @5 10`
C03	`07`	`X`	`ENG`	`@0 Virology @5 10`
C03	`07`	`X`	`SPA`	`@0 Virología @5 10`
C03	`08`	`X`	`FRE`	`@0 Syndrome respiratoire aigu sévère @2 NM @5 14`
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C07	`01`	`X`	`FRE`	`@0 Coronavirus @2 NW`
C07	`01`	`X`	`ENG`	`@0 Coronavirus @2 NW`
C07	`01`	`X`	`SPA`	`@0 Coronavirus @2 NW`
C07	`02`	`X`	`FRE`	`@0 Coronaviridae @2 NW`
C07	`02`	`X`	`ENG`	`@0 Coronaviridae @2 NW`
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C07	`03`	`X`	`FRE`	`@0 Nidovirales @2 NW`
C07	`03`	`X`	`ENG`	`@0 Nidovirales @2 NW`
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C07	`05`	`X`	`ENG`	`@0 Respiratory disease @5 13`
C07	`05`	`X`	`SPA`	`@0 Aparato respiratorio patología @5 13`
C07	`06`	`X`	`FRE`	`@0 Virose`
C07	`06`	`X`	`ENG`	`@0 Viral disease`
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N44	`01`			`@1 OTO`
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Format Inist (serveur)

NO :	PASCAL 05-0205858 INIST
ET :	Clinical evaluation of real-time PCR assays for rapid diagnosis of SARS coronavims during outbreak and post-epidemic periods
AU :	YAM (W. C.); CHAN (K. H.); CHOW (K. H.); POON (L. L. M.); LAM (H. Y.); YUEN (K. Y.); SETO (W. H.); PEIRIS (J. S. M.)
AF :	Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital Compound/Pokfulam/Hong-Kong (1 aut., 2 aut., 3 aut., 4 aut., 5 aut., 6 aut., 7 aut., 8 aut.)
DT :	Publication en série; Niveau analytique
SO :	Journal of clinical virology; ISSN 1386-6532; Pays-Bas; Da. 2005; Vol. 33; No. 1; Pp. 19-24; Bibl. 15 ref.
LA :	Anglais
EA :	Background: The protocols of WHO network laboratories facilitated development of rapid diagnosis for SARS coronavirus (CoV) using reverse transcription (RT)-PCR assays. However, several reports have shown that conventional and real-time PCR assays were very specific for SARS CoV but lack sensitivity depending on the assay, specimen, and time course of disease. Objective: To evaluate an automatic nucleic acid extraction system and two standardized real-time PCR assays for rapid diagnosis of SARS CoV during outbreak and post-epidemic periods in Hong Kong. Study design: Specimens from clinically suspected SARS patients collected during outbreak and post-epidemic periods were tested by an automatic nucleic acid extraction system followed by our first generation conventional RT-PCR and two standardized real-time PCR assays (Artus GmbH, Germany and Roche Diagnostics, Germany). Paired serum samples were assayed for increasing titer against SARS CoV. Results: In the SARS epidemic, Artus and Roche PCR assays exhibited sensitivities of 87% and 85% for respiratory specimens (n = 64), 91% and 88% for stool (n=44), and 82% for urine (n = 29). A specificity of 100% was exhibited by both PCR assays except Artus attained only a 92% specificity for stool. For post-epidemic period, no SARS CoV was identified among 56 respiratory specimens by all PCR assays. Inhibitors to PCR assays were detected at an average rate of 7-8% among 202 clinical specimens. Conclusion: This study highlights the high throughput and performance of automatic RNA extraction in coordination with standardized real-time PCR assays suitable for large-scale routine diagnosis in case of future SARS epidemic. As no SARS CoV was detected among specimens collected during post-epidemic period, the positive predictive value of real-time PCR assays for detection of SARS CoV during low epidemic requires further evaluation.
CC :	002A05C10; 002B05C02J
FD :	Virus syndrome respiratoire aigu sévère; Temps réel; Réaction chaîne polymérase; Diagnostic; Epidémie; Microbiologie; Virologie; Syndrome respiratoire aigu sévère
FG :	Coronavirus; Coronaviridae; Nidovirales; Virus; Appareil respiratoire pathologie; Virose; Infection; Poumon pathologie
ED :	Severe acute respiratory syndrome virus; Real time; Polymerase chain reaction; Diagnosis; Epidemic; Microbiology; Virology; Severe acute respiratory syndrome
EG :	Coronavirus; Coronaviridae; Nidovirales; Virus; Respiratory disease; Viral disease; Infection; Lung disease
SD :	Severe acute respiratory syndrome virus; Tiempo real; Reacción cadena polimerasa; Diagnóstico; Epidemia; Microbiología; Virología; Síndrome respiratorio agudo severo
LO :	INIST-26272.354000125656290040
ID :	05-0205858

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Pascal:05-0205858

Le document en format XML

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<series><title level="j" type="main">Journal of clinical virology</title>
<title level="j" type="abbreviated">J. clin. virol.</title>
<idno type="ISSN">1386-6532</idno>
<imprint><date when="2005">2005</date>
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<seriesStmt><title level="j" type="main">Journal of clinical virology</title>
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<term>Epidemic</term>
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<term>Polymerase chain reaction</term>
<term>Real time</term>
<term>Severe acute respiratory syndrome</term>
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<term>Temps réel</term>
<term>Réaction chaîne polymérase</term>
<term>Diagnostic</term>
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<front><div type="abstract" xml:lang="en">Background: The protocols of WHO network laboratories facilitated development of rapid diagnosis for SARS coronavirus (CoV) using reverse transcription (RT)-PCR assays. However, several reports have shown that conventional and real-time PCR assays were very specific for SARS CoV but lack sensitivity depending on the assay, specimen, and time course of disease. Objective: To evaluate an automatic nucleic acid extraction system and two standardized real-time PCR assays for rapid diagnosis of SARS CoV during outbreak and post-epidemic periods in Hong Kong. Study design: Specimens from clinically suspected SARS patients collected during outbreak and post-epidemic periods were tested by an automatic nucleic acid extraction system followed by our first generation conventional RT-PCR and two standardized real-time PCR assays (Artus GmbH, Germany and Roche Diagnostics, Germany). Paired serum samples were assayed for increasing titer against SARS CoV. Results: In the SARS epidemic, Artus and Roche PCR assays exhibited sensitivities of 87% and 85% for respiratory specimens (n = 64), 91% and 88% for stool (n=44), and 82% for urine (n = 29). A specificity of 100% was exhibited by both PCR assays except Artus attained only a 92% specificity for stool. For post-epidemic period, no SARS CoV was identified among 56 respiratory specimens by all PCR assays. Inhibitors to PCR assays were detected at an average rate of 7-8% among 202 clinical specimens. Conclusion: This study highlights the high throughput and performance of automatic RNA extraction in coordination with standardized real-time PCR assays suitable for large-scale routine diagnosis in case of future SARS epidemic. As no SARS CoV was detected among specimens collected during post-epidemic period, the positive predictive value of real-time PCR assays for detection of SARS CoV during low epidemic requires further evaluation.</div>
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<fA11 i1="01" i2="1"><s1>YAM (W. C.)</s1>
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<fA11 i1="02" i2="1"><s1>CHAN (K. H.)</s1>
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<fA11 i1="05" i2="1"><s1>LAM (H. Y.)</s1>
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<fA11 i1="08" i2="1"><s1>PEIRIS (J. S. M.)</s1>
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<fA14 i1="01"><s1>Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital Compound</s1>
<s2>Pokfulam</s2>
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<sZ>4 aut.</sZ>
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<fC01 i1="01" l="ENG"><s0>Background: The protocols of WHO network laboratories facilitated development of rapid diagnosis for SARS coronavirus (CoV) using reverse transcription (RT)-PCR assays. However, several reports have shown that conventional and real-time PCR assays were very specific for SARS CoV but lack sensitivity depending on the assay, specimen, and time course of disease. Objective: To evaluate an automatic nucleic acid extraction system and two standardized real-time PCR assays for rapid diagnosis of SARS CoV during outbreak and post-epidemic periods in Hong Kong. Study design: Specimens from clinically suspected SARS patients collected during outbreak and post-epidemic periods were tested by an automatic nucleic acid extraction system followed by our first generation conventional RT-PCR and two standardized real-time PCR assays (Artus GmbH, Germany and Roche Diagnostics, Germany). Paired serum samples were assayed for increasing titer against SARS CoV. Results: In the SARS epidemic, Artus and Roche PCR assays exhibited sensitivities of 87% and 85% for respiratory specimens (n = 64), 91% and 88% for stool (n=44), and 82% for urine (n = 29). A specificity of 100% was exhibited by both PCR assays except Artus attained only a 92% specificity for stool. For post-epidemic period, no SARS CoV was identified among 56 respiratory specimens by all PCR assays. Inhibitors to PCR assays were detected at an average rate of 7-8% among 202 clinical specimens. Conclusion: This study highlights the high throughput and performance of automatic RNA extraction in coordination with standardized real-time PCR assays suitable for large-scale routine diagnosis in case of future SARS epidemic. As no SARS CoV was detected among specimens collected during post-epidemic period, the positive predictive value of real-time PCR assays for detection of SARS CoV during low epidemic requires further evaluation.</s0>
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<server><NO>PASCAL 05-0205858 INIST</NO>
<ET>Clinical evaluation of real-time PCR assays for rapid diagnosis of SARS coronavims during outbreak and post-epidemic periods</ET>
<AU>YAM (W. C.); CHAN (K. H.); CHOW (K. H.); POON (L. L. M.); LAM (H. Y.); YUEN (K. Y.); SETO (W. H.); PEIRIS (J. S. M.)</AU>
<AF>Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital Compound/Pokfulam/Hong-Kong (1 aut., 2 aut., 3 aut., 4 aut., 5 aut., 6 aut., 7 aut., 8 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Journal of clinical virology; ISSN 1386-6532; Pays-Bas; Da. 2005; Vol. 33; No. 1; Pp. 19-24; Bibl. 15 ref.</SO>
<LA>Anglais</LA>
<EA>Background: The protocols of WHO network laboratories facilitated development of rapid diagnosis for SARS coronavirus (CoV) using reverse transcription (RT)-PCR assays. However, several reports have shown that conventional and real-time PCR assays were very specific for SARS CoV but lack sensitivity depending on the assay, specimen, and time course of disease. Objective: To evaluate an automatic nucleic acid extraction system and two standardized real-time PCR assays for rapid diagnosis of SARS CoV during outbreak and post-epidemic periods in Hong Kong. Study design: Specimens from clinically suspected SARS patients collected during outbreak and post-epidemic periods were tested by an automatic nucleic acid extraction system followed by our first generation conventional RT-PCR and two standardized real-time PCR assays (Artus GmbH, Germany and Roche Diagnostics, Germany). Paired serum samples were assayed for increasing titer against SARS CoV. Results: In the SARS epidemic, Artus and Roche PCR assays exhibited sensitivities of 87% and 85% for respiratory specimens (n = 64), 91% and 88% for stool (n=44), and 82% for urine (n = 29). A specificity of 100% was exhibited by both PCR assays except Artus attained only a 92% specificity for stool. For post-epidemic period, no SARS CoV was identified among 56 respiratory specimens by all PCR assays. Inhibitors to PCR assays were detected at an average rate of 7-8% among 202 clinical specimens. Conclusion: This study highlights the high throughput and performance of automatic RNA extraction in coordination with standardized real-time PCR assays suitable for large-scale routine diagnosis in case of future SARS epidemic. As no SARS CoV was detected among specimens collected during post-epidemic period, the positive predictive value of real-time PCR assays for detection of SARS CoV during low epidemic requires further evaluation.</EA>
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<ED>Severe acute respiratory syndrome virus; Real time; Polymerase chain reaction; Diagnosis; Epidemic; Microbiology; Virology; Severe acute respiratory syndrome</ED>
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<SD>Severe acute respiratory syndrome virus; Tiempo real; Reacción cadena polimerasa; Diagnóstico; Epidemia; Microbiología; Virología; Síndrome respiratorio agudo severo</SD>
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Clinical evaluation of real-time PCR assays for rapid diagnosis of SARS coronavims during outbreak and post-epidemic periods

Clinical evaluation of real-time PCR assays for rapid diagnosis of SARS coronavims during outbreak and post-epidemic periods

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