SrasV1, PascalFrancis, Corpus, bibRecord, 000697

Identification of novel subgenomic RNAs and noncanonical transcription initiation signals of severe acute respiratory syndrome coronavirus

Identifieur interne : 000697 ( PascalFrancis/Corpus ); précédent : 000696; suivant : 000698

Identification of novel subgenomic RNAs and noncanonical transcription initiation signals of severe acute respiratory syndrome coronavirus

Auteurs : Snawar Hussain ; JI'AN PAN ; YU CHEN ; YALIN YANG ; JING XU ; YU PENG ; YING WU ; ZHAOYANG LI ; YING ZHU ; Po Tien ; DEYIN GUO

Source :

Journal of virology [ 0022-538X ] ; 2005.

RBID : Pascal:05-0209520

Descripteurs français

Pascal (Inist)
- Coronavirus, Identification, Initiation transcription, Microbiologie, Virologie, Syndrome respiratoire aigu sévère.

English descriptors

KwdEn :
- Coronavirus, Identification, Microbiology, Severe acute respiratory syndrome, Transcription initiation, Virology.

Abstract

The expression of the genomic information of severe acute respiratory syndrome coronavirus (SARS CoV) involves synthesis of a nested set of subgenomic RNAs (sgRNAs) by discontinuous transcription. In SARS CoV-infected cells, 10 sgRNAs, including 2 novel ones, were identified, which were predicted to be functional in the expression of 12 open reading frames located in the 3' one-third of the genome. Surprisingly, one new sgRNA could lead to production of a truncated spike protein. Sequence analysis of the leader-body fusion sites of each sgRNA showed that the junction sequences and the corresponding transcription-regulatory sequence (TRS) are unique for each species of sgRNA and are consistent after virus passages. For the two novel sgRNAs, each used a variant of the TRS that has one nucleotide mismatch in the conserved hexanucleotide core (ACGAAC) in the TRS. Coexistence of both plus and minus strands of SARS CoV sgRNAs and evidence for derivation of the sgRNA core sequence from the body core sequence favor the model of discontinuous transcription during minus-strand synthesis. Moreover, one rare species of sgRNA has the junction sequence AAA, indicating that its transcription could result from a noncanonical transcription signal. Taken together, these results provide more insight into the molecular mechanisms of genome expression and subgenomic transcription of SARS CoV.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

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A03		`1`		`@0 J. virol.`
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A11	`01`	`1`		`@1 HUSSAIN (Snawar)`
A11	`02`	`1`		`@1 JI'AN PAN`
A11	`03`	`1`		`@1 YU CHEN`
A11	`04`	`1`		`@1 YALIN YANG`
A11	`05`	`1`		`@1 JING XU`
A11	`06`	`1`		`@1 YU PENG`
A11	`07`	`1`		`@1 YING WU`
A11	`08`	`1`		`@1 ZHAOYANG LI`
A11	`09`	`1`		`@1 YING ZHU`
A11	`10`	`1`		`@1 TIEN (Po)`
A11	`11`	`1`		`@1 DEYIN GUO`
A14	`01`			`@1 Modern Virology Research Centre and National Key Laboratory of urology, College of Life Sciences, Wuhan University @2 Wuhan @3 CHN @Z 1 aut. @Z 2 aut. @Z 3 aut. @Z 4 aut. @Z 5 aut. @Z 6 aut. @Z 7 aut. @Z 8 aut. @Z 9 aut. @Z 10 aut. @Z 11 aut.`
A20				`@1 5288-5295`
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A44				`@0 0000 @1 © 2005 INIST-CNRS. All rights reserved.`
A45				`@0 30 ref.`
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A61				`@0 A`
A64	`01`	`1`		`@0 Journal of virology`
A66	`01`			`@0 USA`
C01	`01`		`ENG`	@0 The expression of the genomic information of severe acute respiratory syndrome coronavirus (SARS CoV) involves synthesis of a nested set of subgenomic RNAs (sgRNAs) by discontinuous transcription. In SARS CoV-infected cells, 10 sgRNAs, including 2 novel ones, were identified, which were predicted to be functional in the expression of 12 open reading frames located in the 3' one-third of the genome. Surprisingly, one new sgRNA could lead to production of a truncated spike protein. Sequence analysis of the leader-body fusion sites of each sgRNA showed that the junction sequences and the corresponding transcription-regulatory sequence (TRS) are unique for each species of sgRNA and are consistent after virus passages. For the two novel sgRNAs, each used a variant of the TRS that has one nucleotide mismatch in the conserved hexanucleotide core (ACGAAC) in the TRS. Coexistence of both plus and minus strands of SARS CoV sgRNAs and evidence for derivation of the sgRNA core sequence from the body core sequence favor the model of discontinuous transcription during minus-strand synthesis. Moreover, one rare species of sgRNA has the junction sequence AAA, indicating that its transcription could result from a noncanonical transcription signal. Taken together, these results provide more insight into the molecular mechanisms of genome expression and subgenomic transcription of SARS CoV.
C02	`01`	`X`		`@0 002A05C10`
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C03	`01`	`X`	`ENG`	`@0 Coronavirus @2 NW @5 01`
C03	`01`	`X`	`SPA`	`@0 Coronavirus @2 NW @5 01`
C03	`02`	`X`	`FRE`	`@0 Identification @5 05`
C03	`02`	`X`	`ENG`	`@0 Identification @5 05`
C03	`02`	`X`	`SPA`	`@0 Identificación @5 05`
C03	`03`	`X`	`FRE`	`@0 Initiation transcription @5 06`
C03	`03`	`X`	`ENG`	`@0 Transcription initiation @5 06`
C03	`03`	`X`	`SPA`	`@0 Iniciación transcripción @5 06`
C03	`04`	`X`	`FRE`	`@0 Microbiologie @5 07`
C03	`04`	`X`	`ENG`	`@0 Microbiology @5 07`
C03	`04`	`X`	`SPA`	`@0 Microbiología @5 07`
C03	`05`	`X`	`FRE`	`@0 Virologie @5 08`
C03	`05`	`X`	`ENG`	`@0 Virology @5 08`
C03	`05`	`X`	`SPA`	`@0 Virología @5 08`
C03	`06`	`X`	`FRE`	`@0 Syndrome respiratoire aigu sévère @2 NM @5 14`
C03	`06`	`X`	`ENG`	`@0 Severe acute respiratory syndrome @2 NM @5 14`
C03	`06`	`X`	`SPA`	`@0 Síndrome respiratorio agudo severo @2 NM @5 14`
C07	`01`	`X`	`FRE`	`@0 Coronaviridae @2 NW`
C07	`01`	`X`	`ENG`	`@0 Coronaviridae @2 NW`
C07	`01`	`X`	`SPA`	`@0 Coronaviridae @2 NW`
C07	`02`	`X`	`FRE`	`@0 Nidovirales @2 NW`
C07	`02`	`X`	`ENG`	`@0 Nidovirales @2 NW`
C07	`02`	`X`	`SPA`	`@0 Nidovirales @2 NW`
C07	`03`	`X`	`FRE`	`@0 Virus @2 NW`
C07	`03`	`X`	`ENG`	`@0 Virus @2 NW`
C07	`03`	`X`	`SPA`	`@0 Virus @2 NW`
C07	`04`	`X`	`FRE`	`@0 Appareil respiratoire pathologie @5 13`
C07	`04`	`X`	`ENG`	`@0 Respiratory disease @5 13`
C07	`04`	`X`	`SPA`	`@0 Aparato respiratorio patología @5 13`
C07	`05`	`X`	`FRE`	`@0 Virose`
C07	`05`	`X`	`ENG`	`@0 Viral disease`
C07	`05`	`X`	`SPA`	`@0 Virosis`
C07	`06`	`X`	`FRE`	`@0 Infection`
C07	`06`	`X`	`ENG`	`@0 Infection`
C07	`06`	`X`	`SPA`	`@0 Infección`
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C07	`07`	`X`	`ENG`	`@0 Lung disease @5 16`
C07	`07`	`X`	`SPA`	`@0 Pulmón patología @5 16`
N21				`@1 143`
N44	`01`			`@1 OTO`
N82				`@1 OTO`

Format Inist (serveur)

NO :	PASCAL 05-0209520 INIST
ET :	Identification of novel subgenomic RNAs and noncanonical transcription initiation signals of severe acute respiratory syndrome coronavirus
AU :	HUSSAIN (Snawar); JI'AN PAN; YU CHEN; YALIN YANG; JING XU; YU PENG; YING WU; ZHAOYANG LI; YING ZHU; TIEN (Po); DEYIN GUO
AF :	Modern Virology Research Centre and National Key Laboratory of urology, College of Life Sciences, Wuhan University/Wuhan/Chine (1 aut., 2 aut., 3 aut., 4 aut., 5 aut., 6 aut., 7 aut., 8 aut., 9 aut., 10 aut., 11 aut.)
DT :	Publication en série; Niveau analytique
SO :	Journal of virology; ISSN 0022-538X; Etats-Unis; Da. 2005; Vol. 79; No. 9; Pp. 5288-5295; Bibl. 30 ref.
LA :	Anglais
EA :	The expression of the genomic information of severe acute respiratory syndrome coronavirus (SARS CoV) involves synthesis of a nested set of subgenomic RNAs (sgRNAs) by discontinuous transcription. In SARS CoV-infected cells, 10 sgRNAs, including 2 novel ones, were identified, which were predicted to be functional in the expression of 12 open reading frames located in the 3' one-third of the genome. Surprisingly, one new sgRNA could lead to production of a truncated spike protein. Sequence analysis of the leader-body fusion sites of each sgRNA showed that the junction sequences and the corresponding transcription-regulatory sequence (TRS) are unique for each species of sgRNA and are consistent after virus passages. For the two novel sgRNAs, each used a variant of the TRS that has one nucleotide mismatch in the conserved hexanucleotide core (ACGAAC) in the TRS. Coexistence of both plus and minus strands of SARS CoV sgRNAs and evidence for derivation of the sgRNA core sequence from the body core sequence favor the model of discontinuous transcription during minus-strand synthesis. Moreover, one rare species of sgRNA has the junction sequence AAA, indicating that its transcription could result from a noncanonical transcription signal. Taken together, these results provide more insight into the molecular mechanisms of genome expression and subgenomic transcription of SARS CoV.
CC :	002A05C10
FD :	Coronavirus; Identification; Initiation transcription; Microbiologie; Virologie; Syndrome respiratoire aigu sévère
FG :	Coronaviridae; Nidovirales; Virus; Appareil respiratoire pathologie; Virose; Infection; Poumon pathologie
ED :	Coronavirus; Identification; Transcription initiation; Microbiology; Virology; Severe acute respiratory syndrome
EG :	Coronaviridae; Nidovirales; Virus; Respiratory disease; Viral disease; Infection; Lung disease
SD :	Coronavirus; Identificación; Iniciación transcripción; Microbiología; Virología; Síndrome respiratorio agudo severo
LO :	INIST-13592.354000125695480060
ID :	05-0209520

Links to Exploration step

Pascal:05-0209520

Le document en format XML

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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Identification of novel subgenomic RNAs and noncanonical transcription initiation signals of severe acute respiratory syndrome coronavirus</title>
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<author><name sortKey="Zhaoyang Li" sort="Zhaoyang Li" uniqKey="Zhaoyang Li" last="Zhaoyang Li">ZHAOYANG LI</name>
<affiliation><inist:fA14 i1="01"><s1>Modern Virology Research Centre and National Key Laboratory of urology, College of Life Sciences, Wuhan University</s1>
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<author><name sortKey="Ying Zhu" sort="Ying Zhu" uniqKey="Ying Zhu" last="Ying Zhu">YING ZHU</name>
<affiliation><inist:fA14 i1="01"><s1>Modern Virology Research Centre and National Key Laboratory of urology, College of Life Sciences, Wuhan University</s1>
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<author><name sortKey="Tien, Po" sort="Tien, Po" uniqKey="Tien P" first="Po" last="Tien">Po Tien</name>
<affiliation><inist:fA14 i1="01"><s1>Modern Virology Research Centre and National Key Laboratory of urology, College of Life Sciences, Wuhan University</s1>
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<series><title level="j" type="main">Journal of virology</title>
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<front><div type="abstract" xml:lang="en">The expression of the genomic information of severe acute respiratory syndrome coronavirus (SARS CoV) involves synthesis of a nested set of subgenomic RNAs (sgRNAs) by discontinuous transcription. In SARS CoV-infected cells, 10 sgRNAs, including 2 novel ones, were identified, which were predicted to be functional in the expression of 12 open reading frames located in the 3' one-third of the genome. Surprisingly, one new sgRNA could lead to production of a truncated spike protein. Sequence analysis of the leader-body fusion sites of each sgRNA showed that the junction sequences and the corresponding transcription-regulatory sequence (TRS) are unique for each species of sgRNA and are consistent after virus passages. For the two novel sgRNAs, each used a variant of the TRS that has one nucleotide mismatch in the conserved hexanucleotide core (ACGAAC) in the TRS. Coexistence of both plus and minus strands of SARS CoV sgRNAs and evidence for derivation of the sgRNA core sequence from the body core sequence favor the model of discontinuous transcription during minus-strand synthesis. Moreover, one rare species of sgRNA has the junction sequence AAA, indicating that its transcription could result from a noncanonical transcription signal. Taken together, these results provide more insight into the molecular mechanisms of genome expression and subgenomic transcription of SARS CoV.</div>
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<ET>Identification of novel subgenomic RNAs and noncanonical transcription initiation signals of severe acute respiratory syndrome coronavirus</ET>
<AU>HUSSAIN (Snawar); JI'AN PAN; YU CHEN; YALIN YANG; JING XU; YU PENG; YING WU; ZHAOYANG LI; YING ZHU; TIEN (Po); DEYIN GUO</AU>
<AF>Modern Virology Research Centre and National Key Laboratory of urology, College of Life Sciences, Wuhan University/Wuhan/Chine (1 aut., 2 aut., 3 aut., 4 aut., 5 aut., 6 aut., 7 aut., 8 aut., 9 aut., 10 aut., 11 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
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<LA>Anglais</LA>
<EA>The expression of the genomic information of severe acute respiratory syndrome coronavirus (SARS CoV) involves synthesis of a nested set of subgenomic RNAs (sgRNAs) by discontinuous transcription. In SARS CoV-infected cells, 10 sgRNAs, including 2 novel ones, were identified, which were predicted to be functional in the expression of 12 open reading frames located in the 3' one-third of the genome. Surprisingly, one new sgRNA could lead to production of a truncated spike protein. Sequence analysis of the leader-body fusion sites of each sgRNA showed that the junction sequences and the corresponding transcription-regulatory sequence (TRS) are unique for each species of sgRNA and are consistent after virus passages. For the two novel sgRNAs, each used a variant of the TRS that has one nucleotide mismatch in the conserved hexanucleotide core (ACGAAC) in the TRS. Coexistence of both plus and minus strands of SARS CoV sgRNAs and evidence for derivation of the sgRNA core sequence from the body core sequence favor the model of discontinuous transcription during minus-strand synthesis. Moreover, one rare species of sgRNA has the junction sequence AAA, indicating that its transcription could result from a noncanonical transcription signal. Taken together, these results provide more insight into the molecular mechanisms of genome expression and subgenomic transcription of SARS CoV.</EA>
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<EG>Coronaviridae; Nidovirales; Virus; Respiratory disease; Viral disease; Infection; Lung disease</EG>
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Identification of novel subgenomic RNAs and noncanonical transcription initiation signals of severe acute respiratory syndrome coronavirus

Identification of novel subgenomic RNAs and noncanonical transcription initiation signals of severe acute respiratory syndrome coronavirus

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