SrasV1, PascalFrancis, Corpus, bibRecord, 000687

Differential maturation and subcellular localization of severe acute respiratory syndrome coronavirus surface proteins S, M and E

Identifieur interne : 000687 ( PascalFrancis/Corpus ); précédent : 000686; suivant : 000688

Differential maturation and subcellular localization of severe acute respiratory syndrome coronavirus surface proteins S, M and E

Auteurs : Baatrice Nal ; Cheman Chan ; Francois Kien ; Lewis Siu ; Jane Tse ; Kid Chu ; Jason Kam ; Isabelle Staropoli ; Bernadette Crescenzo-Chaigne ; Nicolas Escriou ; Sylvie Van Der Werf ; Kwok-Yung Yuen ; Ralf Altmeyer

Source :

Journal of general virology [ 0022-1317 ] ; 2005.

RBID : Pascal:05-0229117

Descripteurs français

Pascal (Inist)
- Coronavirus, Localisation, Protéine, Microbiologie, Virologie, Syndrome respiratoire aigu sévère.

English descriptors

KwdEn :
- Coronavirus, Localization, Microbiology, Protein, Severe acute respiratory syndrome, Virology.

Abstract

Post-translational modifications and correct subcellular localization of viral structural proteins are prerequisites for assembly and budding of enveloped viruses. Coronaviruses, like the severe acute respiratory syndrome-associated virus (SARS-CoV), bud from the endoplasmic reticulum-Golgi intermediate compartment. In this study, the subcellular distribution and maturation of SARS-CoV surface proteins S, M and E were analysed by using C-terminally tagged proteins. As early as 30 min post-entry into the endoplasmic reticulum, high-mannosylated S assembles into trimers prior to acquisition of complex N-glycans in the Golgi. Like S, M acquires high-mannose N-glycans that are subsequently modified into complex N-glycans in the Golgi. The N-glycosylation profile and the absence of O-glycosylation on M protein relate SARS-CoV to the previously described group 1 and 3 coronaviruses. Immunofluorescence analysis shows that S is detected in several compartments along the secretory pathway from the endoplasmic reticulum to the plasma membrane while M predominantly localizes in the Golgi, where it accumulates, and in trafficking vesicles. The E protein is not glycosylated. Pulse-chase labelling and confocal microscopy in the presence of protein translation inhibitor cycloheximide revealed that the E protein has a short half-life of 30 min. E protein is found in bright perinuclear patches colocalizing with endoplasmic reticulum markers. In conclusion, SARS-CoV surface proteins S, M and E show differential subcellular localizations when expressed alone suggesting that additional cellular or viral factors might be required for coordinated trafficking to the virus assembly site in the endoplasmic reticulum-Golgi intermediate compartment.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

A01	`01`	`1`		`@0 0022-1317`
A02	`01`			`@0 JGVIAY`
A03		`1`		`@0 J. gen. virol.`
A05				`@2 86`
A06				`@3 p.5`
A08	`01`	`1`	`ENG`	`@1 Differential maturation and subcellular localization of severe acute respiratory syndrome coronavirus surface proteins S, M and E`
A11	`01`	`1`		`@1 NAL (Baatrice)`
A11	`02`	`1`		`@1 CHAN (Cheman)`
A11	`03`	`1`		`@1 KIEN (Francois)`
A11	`04`	`1`		`@1 SIU (Lewis)`
A11	`05`	`1`		`@1 TSE (Jane)`
A11	`06`	`1`		`@1 CHU (Kid)`
A11	`07`	`1`		`@1 KAM (Jason)`
A11	`08`	`1`		`@1 STAROPOLI (Isabelle)`
A11	`09`	`1`		`@1 CRESCENZO-CHAIGNE (Bernadette)`
A11	`10`	`1`		`@1 ESCRIOU (Nicolas)`
A11	`11`	`1`		`@1 VAN DER WERF (Sylvie)`
A11	`12`	`1`		`@1 YUEN (Kwok-Yung)`
A11	`13`	`1`		`@1 ALTMEYER (Ralf)`
A14	`01`			`@1 HKU-Pasteur Research Centre, 8 Sassoon Road @3 HKG @Z 1 aut. @Z 2 aut. @Z 3 aut. @Z 4 aut. @Z 5 aut. @Z 6 aut. @Z 7 aut. @Z 13 aut.`
A14	`02`			`@1 Unité d'lmmunologie Virale, Institut Pasteur, 25 rue du Dr Roux @2 Paris @3 FRA @Z 8 aut.`
A14	`03`			`@1 Unité de Génétique Moléculaire des Virus Respiratoires, Institut Pasteur, 25 rue du Dr Roux @2 Paris @3 FRA @Z 9 aut. @Z 10 aut. @Z 11 aut.`
A14	`04`			`@1 Department of Microbiology, The University of Hong Kong @3 HKG @Z 12 aut.`
A20				`@1 1423-1434`
A21				`@1 2005`
A23	`01`			`@0 ENG`
A43	`01`			`@1 INIST @2 13533 @5 354000129444440210`
A44				`@0 0000 @1 © 2005 INIST-CNRS. All rights reserved.`
A45				`@0 2 p.1/4`
A47	`01`	`1`		`@0 05-0229117`
A60				`@1 P`
A61				`@0 A`
A64	`01`	`1`		`@0 Journal of general virology`
A66	`01`			`@0 GBR`
C01	`01`		`ENG`	@0 Post-translational modifications and correct subcellular localization of viral structural proteins are prerequisites for assembly and budding of enveloped viruses. Coronaviruses, like the severe acute respiratory syndrome-associated virus (SARS-CoV), bud from the endoplasmic reticulum-Golgi intermediate compartment. In this study, the subcellular distribution and maturation of SARS-CoV surface proteins S, M and E were analysed by using C-terminally tagged proteins. As early as 30 min post-entry into the endoplasmic reticulum, high-mannosylated S assembles into trimers prior to acquisition of complex N-glycans in the Golgi. Like S, M acquires high-mannose N-glycans that are subsequently modified into complex N-glycans in the Golgi. The N-glycosylation profile and the absence of O-glycosylation on M protein relate SARS-CoV to the previously described group 1 and 3 coronaviruses. Immunofluorescence analysis shows that S is detected in several compartments along the secretory pathway from the endoplasmic reticulum to the plasma membrane while M predominantly localizes in the Golgi, where it accumulates, and in trafficking vesicles. The E protein is not glycosylated. Pulse-chase labelling and confocal microscopy in the presence of protein translation inhibitor cycloheximide revealed that the E protein has a short half-life of 30 min. E protein is found in bright perinuclear patches colocalizing with endoplasmic reticulum markers. In conclusion, SARS-CoV surface proteins S, M and E show differential subcellular localizations when expressed alone suggesting that additional cellular or viral factors might be required for coordinated trafficking to the virus assembly site in the endoplasmic reticulum-Golgi intermediate compartment.
C02	`01`	`X`		`@0 002A05C10`
C03	`01`	`X`	`FRE`	`@0 Coronavirus @2 NW @5 01`
C03	`01`	`X`	`ENG`	`@0 Coronavirus @2 NW @5 01`
C03	`01`	`X`	`SPA`	`@0 Coronavirus @2 NW @5 01`
C03	`02`	`X`	`FRE`	`@0 Localisation @5 05`
C03	`02`	`X`	`ENG`	`@0 Localization @5 05`
C03	`02`	`X`	`SPA`	`@0 Localización @5 05`
C03	`03`	`X`	`FRE`	`@0 Protéine @5 06`
C03	`03`	`X`	`ENG`	`@0 Protein @5 06`
C03	`03`	`X`	`SPA`	`@0 Proteína @5 06`
C03	`04`	`X`	`FRE`	`@0 Microbiologie @5 07`
C03	`04`	`X`	`ENG`	`@0 Microbiology @5 07`
C03	`04`	`X`	`SPA`	`@0 Microbiología @5 07`
C03	`05`	`X`	`FRE`	`@0 Virologie @5 08`
C03	`05`	`X`	`ENG`	`@0 Virology @5 08`
C03	`05`	`X`	`SPA`	`@0 Virología @5 08`
C03	`06`	`X`	`FRE`	`@0 Syndrome respiratoire aigu sévère @2 NM @5 14`
C03	`06`	`X`	`ENG`	`@0 Severe acute respiratory syndrome @2 NM @5 14`
C03	`06`	`X`	`SPA`	`@0 Síndrome respiratorio agudo severo @2 NM @5 14`
C07	`01`	`X`	`FRE`	`@0 Coronaviridae @2 NW`
C07	`01`	`X`	`ENG`	`@0 Coronaviridae @2 NW`
C07	`01`	`X`	`SPA`	`@0 Coronaviridae @2 NW`
C07	`02`	`X`	`FRE`	`@0 Nidovirales @2 NW`
C07	`02`	`X`	`ENG`	`@0 Nidovirales @2 NW`
C07	`02`	`X`	`SPA`	`@0 Nidovirales @2 NW`
C07	`03`	`X`	`FRE`	`@0 Virus @2 NW`
C07	`03`	`X`	`ENG`	`@0 Virus @2 NW`
C07	`03`	`X`	`SPA`	`@0 Virus @2 NW`
C07	`04`	`X`	`FRE`	`@0 Appareil respiratoire pathologie @5 13`
C07	`04`	`X`	`ENG`	`@0 Respiratory disease @5 13`
C07	`04`	`X`	`SPA`	`@0 Aparato respiratorio patología @5 13`
C07	`05`	`X`	`FRE`	`@0 Virose`
C07	`05`	`X`	`ENG`	`@0 Viral disease`
C07	`05`	`X`	`SPA`	`@0 Virosis`
C07	`06`	`X`	`FRE`	`@0 Infection`
C07	`06`	`X`	`ENG`	`@0 Infection`
C07	`06`	`X`	`SPA`	`@0 Infección`
C07	`07`	`X`	`FRE`	`@0 Poumon pathologie @5 16`
C07	`07`	`X`	`ENG`	`@0 Lung disease @5 16`
C07	`07`	`X`	`SPA`	`@0 Pulmón patología @5 16`
N21				`@1 157`
N44	`01`			`@1 OTO`
N82				`@1 OTO`

Format Inist (serveur)

NO :	PASCAL 05-0229117 INIST
ET :	Differential maturation and subcellular localization of severe acute respiratory syndrome coronavirus surface proteins S, M and E
AU :	NAL (Baatrice); CHAN (Cheman); KIEN (Francois); SIU (Lewis); TSE (Jane); CHU (Kid); KAM (Jason); STAROPOLI (Isabelle); CRESCENZO-CHAIGNE (Bernadette); ESCRIOU (Nicolas); VAN DER WERF (Sylvie); YUEN (Kwok-Yung); ALTMEYER (Ralf)
AF :	HKU-Pasteur Research Centre, 8 Sassoon Road/Hong-Kong (1 aut., 2 aut., 3 aut., 4 aut., 5 aut., 6 aut., 7 aut., 13 aut.); Unité d'lmmunologie Virale, Institut Pasteur, 25 rue du Dr Roux/Paris/France (8 aut.); Unité de Génétique Moléculaire des Virus Respiratoires, Institut Pasteur, 25 rue du Dr Roux/Paris/France (9 aut., 10 aut., 11 aut.); Department of Microbiology, The University of Hong Kong/Hong-Kong (12 aut.)
DT :	Publication en série; Niveau analytique
SO :	Journal of general virology; ISSN 0022-1317; Coden JGVIAY; Royaume-Uni; Da. 2005; Vol. 86; No. p.5; Pp. 1423-1434; Bibl. 2 p.1/4
LA :	Anglais
EA :	Post-translational modifications and correct subcellular localization of viral structural proteins are prerequisites for assembly and budding of enveloped viruses. Coronaviruses, like the severe acute respiratory syndrome-associated virus (SARS-CoV), bud from the endoplasmic reticulum-Golgi intermediate compartment. In this study, the subcellular distribution and maturation of SARS-CoV surface proteins S, M and E were analysed by using C-terminally tagged proteins. As early as 30 min post-entry into the endoplasmic reticulum, high-mannosylated S assembles into trimers prior to acquisition of complex N-glycans in the Golgi. Like S, M acquires high-mannose N-glycans that are subsequently modified into complex N-glycans in the Golgi. The N-glycosylation profile and the absence of O-glycosylation on M protein relate SARS-CoV to the previously described group 1 and 3 coronaviruses. Immunofluorescence analysis shows that S is detected in several compartments along the secretory pathway from the endoplasmic reticulum to the plasma membrane while M predominantly localizes in the Golgi, where it accumulates, and in trafficking vesicles. The E protein is not glycosylated. Pulse-chase labelling and confocal microscopy in the presence of protein translation inhibitor cycloheximide revealed that the E protein has a short half-life of 30 min. E protein is found in bright perinuclear patches colocalizing with endoplasmic reticulum markers. In conclusion, SARS-CoV surface proteins S, M and E show differential subcellular localizations when expressed alone suggesting that additional cellular or viral factors might be required for coordinated trafficking to the virus assembly site in the endoplasmic reticulum-Golgi intermediate compartment.
CC :	002A05C10
FD :	Coronavirus; Localisation; Protéine; Microbiologie; Virologie; Syndrome respiratoire aigu sévère
FG :	Coronaviridae; Nidovirales; Virus; Appareil respiratoire pathologie; Virose; Infection; Poumon pathologie
ED :	Coronavirus; Localization; Protein; Microbiology; Virology; Severe acute respiratory syndrome
EG :	Coronaviridae; Nidovirales; Virus; Respiratory disease; Viral disease; Infection; Lung disease
SD :	Coronavirus; Localización; Proteína; Microbiología; Virología; Síndrome respiratorio agudo severo
LO :	INIST-13533.354000129444440210
ID :	05-0229117

Links to Exploration step

Pascal:05-0229117

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Differential maturation and subcellular localization of severe acute respiratory syndrome coronavirus surface proteins S, M and E</title>
<author><name sortKey="Nal, Baatrice" sort="Nal, Baatrice" uniqKey="Nal B" first="Baatrice" last="Nal">Baatrice Nal</name>
<affiliation><inist:fA14 i1="01"><s1>HKU-Pasteur Research Centre, 8 Sassoon Road</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>13 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Chan, Cheman" sort="Chan, Cheman" uniqKey="Chan C" first="Cheman" last="Chan">Cheman Chan</name>
<affiliation><inist:fA14 i1="01"><s1>HKU-Pasteur Research Centre, 8 Sassoon Road</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>13 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Kien, Francois" sort="Kien, Francois" uniqKey="Kien F" first="Francois" last="Kien">Francois Kien</name>
<affiliation><inist:fA14 i1="01"><s1>HKU-Pasteur Research Centre, 8 Sassoon Road</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>13 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Siu, Lewis" sort="Siu, Lewis" uniqKey="Siu L" first="Lewis" last="Siu">Lewis Siu</name>
<affiliation><inist:fA14 i1="01"><s1>HKU-Pasteur Research Centre, 8 Sassoon Road</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>13 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Tse, Jane" sort="Tse, Jane" uniqKey="Tse J" first="Jane" last="Tse">Jane Tse</name>
<affiliation><inist:fA14 i1="01"><s1>HKU-Pasteur Research Centre, 8 Sassoon Road</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>13 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Chu, Kid" sort="Chu, Kid" uniqKey="Chu K" first="Kid" last="Chu">Kid Chu</name>
<affiliation><inist:fA14 i1="01"><s1>HKU-Pasteur Research Centre, 8 Sassoon Road</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>13 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Kam, Jason" sort="Kam, Jason" uniqKey="Kam J" first="Jason" last="Kam">Jason Kam</name>
<affiliation><inist:fA14 i1="01"><s1>HKU-Pasteur Research Centre, 8 Sassoon Road</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>13 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Staropoli, Isabelle" sort="Staropoli, Isabelle" uniqKey="Staropoli I" first="Isabelle" last="Staropoli">Isabelle Staropoli</name>
<affiliation><inist:fA14 i1="02"><s1>Unité d'lmmunologie Virale, Institut Pasteur, 25 rue du Dr Roux</s1>
<s2>Paris</s2>
<s3>FRA</s3>
<sZ>8 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Crescenzo Chaigne, Bernadette" sort="Crescenzo Chaigne, Bernadette" uniqKey="Crescenzo Chaigne B" first="Bernadette" last="Crescenzo-Chaigne">Bernadette Crescenzo-Chaigne</name>
<affiliation><inist:fA14 i1="03"><s1>Unité de Génétique Moléculaire des Virus Respiratoires, Institut Pasteur, 25 rue du Dr Roux</s1>
<s2>Paris</s2>
<s3>FRA</s3>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Escriou, Nicolas" sort="Escriou, Nicolas" uniqKey="Escriou N" first="Nicolas" last="Escriou">Nicolas Escriou</name>
<affiliation><inist:fA14 i1="03"><s1>Unité de Génétique Moléculaire des Virus Respiratoires, Institut Pasteur, 25 rue du Dr Roux</s1>
<s2>Paris</s2>
<s3>FRA</s3>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Van Der Werf, Sylvie" sort="Van Der Werf, Sylvie" uniqKey="Van Der Werf S" first="Sylvie" last="Van Der Werf">Sylvie Van Der Werf</name>
<affiliation><inist:fA14 i1="03"><s1>Unité de Génétique Moléculaire des Virus Respiratoires, Institut Pasteur, 25 rue du Dr Roux</s1>
<s2>Paris</s2>
<s3>FRA</s3>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name>
<affiliation><inist:fA14 i1="04"><s1>Department of Microbiology, The University of Hong Kong</s1>
<s3>HKG</s3>
<sZ>12 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Altmeyer, Ralf" sort="Altmeyer, Ralf" uniqKey="Altmeyer R" first="Ralf" last="Altmeyer">Ralf Altmeyer</name>
<affiliation><inist:fA14 i1="01"><s1>HKU-Pasteur Research Centre, 8 Sassoon Road</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>13 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">05-0229117</idno>
<date when="2005">2005</date>
<idno type="stanalyst">PASCAL 05-0229117 INIST</idno>
<idno type="RBID">Pascal:05-0229117</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000687</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Differential maturation and subcellular localization of severe acute respiratory syndrome coronavirus surface proteins S, M and E</title>
<author><name sortKey="Nal, Baatrice" sort="Nal, Baatrice" uniqKey="Nal B" first="Baatrice" last="Nal">Baatrice Nal</name>
<affiliation><inist:fA14 i1="01"><s1>HKU-Pasteur Research Centre, 8 Sassoon Road</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>13 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Chan, Cheman" sort="Chan, Cheman" uniqKey="Chan C" first="Cheman" last="Chan">Cheman Chan</name>
<affiliation><inist:fA14 i1="01"><s1>HKU-Pasteur Research Centre, 8 Sassoon Road</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>13 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Kien, Francois" sort="Kien, Francois" uniqKey="Kien F" first="Francois" last="Kien">Francois Kien</name>
<affiliation><inist:fA14 i1="01"><s1>HKU-Pasteur Research Centre, 8 Sassoon Road</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>13 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Siu, Lewis" sort="Siu, Lewis" uniqKey="Siu L" first="Lewis" last="Siu">Lewis Siu</name>
<affiliation><inist:fA14 i1="01"><s1>HKU-Pasteur Research Centre, 8 Sassoon Road</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>13 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Tse, Jane" sort="Tse, Jane" uniqKey="Tse J" first="Jane" last="Tse">Jane Tse</name>
<affiliation><inist:fA14 i1="01"><s1>HKU-Pasteur Research Centre, 8 Sassoon Road</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>13 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Chu, Kid" sort="Chu, Kid" uniqKey="Chu K" first="Kid" last="Chu">Kid Chu</name>
<affiliation><inist:fA14 i1="01"><s1>HKU-Pasteur Research Centre, 8 Sassoon Road</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>13 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Kam, Jason" sort="Kam, Jason" uniqKey="Kam J" first="Jason" last="Kam">Jason Kam</name>
<affiliation><inist:fA14 i1="01"><s1>HKU-Pasteur Research Centre, 8 Sassoon Road</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>13 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Staropoli, Isabelle" sort="Staropoli, Isabelle" uniqKey="Staropoli I" first="Isabelle" last="Staropoli">Isabelle Staropoli</name>
<affiliation><inist:fA14 i1="02"><s1>Unité d'lmmunologie Virale, Institut Pasteur, 25 rue du Dr Roux</s1>
<s2>Paris</s2>
<s3>FRA</s3>
<sZ>8 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Crescenzo Chaigne, Bernadette" sort="Crescenzo Chaigne, Bernadette" uniqKey="Crescenzo Chaigne B" first="Bernadette" last="Crescenzo-Chaigne">Bernadette Crescenzo-Chaigne</name>
<affiliation><inist:fA14 i1="03"><s1>Unité de Génétique Moléculaire des Virus Respiratoires, Institut Pasteur, 25 rue du Dr Roux</s1>
<s2>Paris</s2>
<s3>FRA</s3>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Escriou, Nicolas" sort="Escriou, Nicolas" uniqKey="Escriou N" first="Nicolas" last="Escriou">Nicolas Escriou</name>
<affiliation><inist:fA14 i1="03"><s1>Unité de Génétique Moléculaire des Virus Respiratoires, Institut Pasteur, 25 rue du Dr Roux</s1>
<s2>Paris</s2>
<s3>FRA</s3>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Van Der Werf, Sylvie" sort="Van Der Werf, Sylvie" uniqKey="Van Der Werf S" first="Sylvie" last="Van Der Werf">Sylvie Van Der Werf</name>
<affiliation><inist:fA14 i1="03"><s1>Unité de Génétique Moléculaire des Virus Respiratoires, Institut Pasteur, 25 rue du Dr Roux</s1>
<s2>Paris</s2>
<s3>FRA</s3>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name>
<affiliation><inist:fA14 i1="04"><s1>Department of Microbiology, The University of Hong Kong</s1>
<s3>HKG</s3>
<sZ>12 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Altmeyer, Ralf" sort="Altmeyer, Ralf" uniqKey="Altmeyer R" first="Ralf" last="Altmeyer">Ralf Altmeyer</name>
<affiliation><inist:fA14 i1="01"><s1>HKU-Pasteur Research Centre, 8 Sassoon Road</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>13 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Journal of general virology</title>
<title level="j" type="abbreviated">J. gen. virol.</title>
<idno type="ISSN">0022-1317</idno>
<imprint><date when="2005">2005</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Journal of general virology</title>
<title level="j" type="abbreviated">J. gen. virol.</title>
<idno type="ISSN">0022-1317</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Coronavirus</term>
<term>Localization</term>
<term>Microbiology</term>
<term>Protein</term>
<term>Severe acute respiratory syndrome</term>
<term>Virology</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Coronavirus</term>
<term>Localisation</term>
<term>Protéine</term>
<term>Microbiologie</term>
<term>Virologie</term>
<term>Syndrome respiratoire aigu sévère</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Post-translational modifications and correct subcellular localization of viral structural proteins are prerequisites for assembly and budding of enveloped viruses. Coronaviruses, like the severe acute respiratory syndrome-associated virus (SARS-CoV), bud from the endoplasmic reticulum-Golgi intermediate compartment. In this study, the subcellular distribution and maturation of SARS-CoV surface proteins S, M and E were analysed by using C-terminally tagged proteins. As early as 30 min post-entry into the endoplasmic reticulum, high-mannosylated S assembles into trimers prior to acquisition of complex N-glycans in the Golgi. Like S, M acquires high-mannose N-glycans that are subsequently modified into complex N-glycans in the Golgi. The N-glycosylation profile and the absence of O-glycosylation on M protein relate SARS-CoV to the previously described group 1 and 3 coronaviruses. Immunofluorescence analysis shows that S is detected in several compartments along the secretory pathway from the endoplasmic reticulum to the plasma membrane while M predominantly localizes in the Golgi, where it accumulates, and in trafficking vesicles. The E protein is not glycosylated. Pulse-chase labelling and confocal microscopy in the presence of protein translation inhibitor cycloheximide revealed that the E protein has a short half-life of 30 min. E protein is found in bright perinuclear patches colocalizing with endoplasmic reticulum markers. In conclusion, SARS-CoV surface proteins S, M and E show differential subcellular localizations when expressed alone suggesting that additional cellular or viral factors might be required for coordinated trafficking to the virus assembly site in the endoplasmic reticulum-Golgi intermediate compartment.</div>
</front>
</TEI>
<inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0022-1317</s0>
</fA01>
<fA02 i1="01"><s0>JGVIAY</s0>
</fA02>
<fA03 i2="1"><s0>J. gen. virol.</s0>
</fA03>
<fA05><s2>86</s2>
</fA05>
<fA06><s3>p.5</s3>
</fA06>
<fA08 i1="01" i2="1" l="ENG"><s1>Differential maturation and subcellular localization of severe acute respiratory syndrome coronavirus surface proteins S, M and E</s1>
</fA08>
<fA11 i1="01" i2="1"><s1>NAL (Baatrice)</s1>
</fA11>
<fA11 i1="02" i2="1"><s1>CHAN (Cheman)</s1>
</fA11>
<fA11 i1="03" i2="1"><s1>KIEN (Francois)</s1>
</fA11>
<fA11 i1="04" i2="1"><s1>SIU (Lewis)</s1>
</fA11>
<fA11 i1="05" i2="1"><s1>TSE (Jane)</s1>
</fA11>
<fA11 i1="06" i2="1"><s1>CHU (Kid)</s1>
</fA11>
<fA11 i1="07" i2="1"><s1>KAM (Jason)</s1>
</fA11>
<fA11 i1="08" i2="1"><s1>STAROPOLI (Isabelle)</s1>
</fA11>
<fA11 i1="09" i2="1"><s1>CRESCENZO-CHAIGNE (Bernadette)</s1>
</fA11>
<fA11 i1="10" i2="1"><s1>ESCRIOU (Nicolas)</s1>
</fA11>
<fA11 i1="11" i2="1"><s1>VAN DER WERF (Sylvie)</s1>
</fA11>
<fA11 i1="12" i2="1"><s1>YUEN (Kwok-Yung)</s1>
</fA11>
<fA11 i1="13" i2="1"><s1>ALTMEYER (Ralf)</s1>
</fA11>
<fA14 i1="01"><s1>HKU-Pasteur Research Centre, 8 Sassoon Road</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>13 aut.</sZ>
</fA14>
<fA14 i1="02"><s1>Unité d'lmmunologie Virale, Institut Pasteur, 25 rue du Dr Roux</s1>
<s2>Paris</s2>
<s3>FRA</s3>
<sZ>8 aut.</sZ>
</fA14>
<fA14 i1="03"><s1>Unité de Génétique Moléculaire des Virus Respiratoires, Institut Pasteur, 25 rue du Dr Roux</s1>
<s2>Paris</s2>
<s3>FRA</s3>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</fA14>
<fA14 i1="04"><s1>Department of Microbiology, The University of Hong Kong</s1>
<s3>HKG</s3>
<sZ>12 aut.</sZ>
</fA14>
<fA20><s1>1423-1434</s1>
</fA20>
<fA21><s1>2005</s1>
</fA21>
<fA23 i1="01"><s0>ENG</s0>
</fA23>
<fA43 i1="01"><s1>INIST</s1>
<s2>13533</s2>
<s5>354000129444440210</s5>
</fA43>
<fA44><s0>0000</s0>
<s1>© 2005 INIST-CNRS. All rights reserved.</s1>
</fA44>
<fA45><s0>2 p.1/4</s0>
</fA45>
<fA47 i1="01" i2="1"><s0>05-0229117</s0>
</fA47>
<fA60><s1>P</s1>
</fA60>
<fA61><s0>A</s0>
</fA61>
<fA64 i1="01" i2="1"><s0>Journal of general virology</s0>
</fA64>
<fA66 i1="01"><s0>GBR</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>Post-translational modifications and correct subcellular localization of viral structural proteins are prerequisites for assembly and budding of enveloped viruses. Coronaviruses, like the severe acute respiratory syndrome-associated virus (SARS-CoV), bud from the endoplasmic reticulum-Golgi intermediate compartment. In this study, the subcellular distribution and maturation of SARS-CoV surface proteins S, M and E were analysed by using C-terminally tagged proteins. As early as 30 min post-entry into the endoplasmic reticulum, high-mannosylated S assembles into trimers prior to acquisition of complex N-glycans in the Golgi. Like S, M acquires high-mannose N-glycans that are subsequently modified into complex N-glycans in the Golgi. The N-glycosylation profile and the absence of O-glycosylation on M protein relate SARS-CoV to the previously described group 1 and 3 coronaviruses. Immunofluorescence analysis shows that S is detected in several compartments along the secretory pathway from the endoplasmic reticulum to the plasma membrane while M predominantly localizes in the Golgi, where it accumulates, and in trafficking vesicles. The E protein is not glycosylated. Pulse-chase labelling and confocal microscopy in the presence of protein translation inhibitor cycloheximide revealed that the E protein has a short half-life of 30 min. E protein is found in bright perinuclear patches colocalizing with endoplasmic reticulum markers. In conclusion, SARS-CoV surface proteins S, M and E show differential subcellular localizations when expressed alone suggesting that additional cellular or viral factors might be required for coordinated trafficking to the virus assembly site in the endoplasmic reticulum-Golgi intermediate compartment.</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>002A05C10</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Localisation</s0>
<s5>05</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Localization</s0>
<s5>05</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Localización</s0>
<s5>05</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Protéine</s0>
<s5>06</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Protein</s0>
<s5>06</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Proteína</s0>
<s5>06</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Microbiologie</s0>
<s5>07</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Microbiology</s0>
<s5>07</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Microbiología</s0>
<s5>07</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Virologie</s0>
<s5>08</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Virology</s0>
<s5>08</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Virología</s0>
<s5>08</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Syndrome respiratoire aigu sévère</s0>
<s2>NM</s2>
<s5>14</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Severe acute respiratory syndrome</s0>
<s2>NM</s2>
<s5>14</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Síndrome respiratorio agudo severo</s0>
<s2>NM</s2>
<s5>14</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Appareil respiratoire pathologie</s0>
<s5>13</s5>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Respiratory disease</s0>
<s5>13</s5>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Aparato respiratorio patología</s0>
<s5>13</s5>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Virose</s0>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Viral disease</s0>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Virosis</s0>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Infection</s0>
</fC07>
<fC07 i1="06" i2="X" l="ENG"><s0>Infection</s0>
</fC07>
<fC07 i1="06" i2="X" l="SPA"><s0>Infección</s0>
</fC07>
<fC07 i1="07" i2="X" l="FRE"><s0>Poumon pathologie</s0>
<s5>16</s5>
</fC07>
<fC07 i1="07" i2="X" l="ENG"><s0>Lung disease</s0>
<s5>16</s5>
</fC07>
<fC07 i1="07" i2="X" l="SPA"><s0>Pulmón patología</s0>
<s5>16</s5>
</fC07>
<fN21><s1>157</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
</standard>
<server><NO>PASCAL 05-0229117 INIST</NO>
<ET>Differential maturation and subcellular localization of severe acute respiratory syndrome coronavirus surface proteins S, M and E</ET>
<AU>NAL (Baatrice); CHAN (Cheman); KIEN (Francois); SIU (Lewis); TSE (Jane); CHU (Kid); KAM (Jason); STAROPOLI (Isabelle); CRESCENZO-CHAIGNE (Bernadette); ESCRIOU (Nicolas); VAN DER WERF (Sylvie); YUEN (Kwok-Yung); ALTMEYER (Ralf)</AU>
<AF>HKU-Pasteur Research Centre, 8 Sassoon Road/Hong-Kong (1 aut., 2 aut., 3 aut., 4 aut., 5 aut., 6 aut., 7 aut., 13 aut.); Unité d'lmmunologie Virale, Institut Pasteur, 25 rue du Dr Roux/Paris/France (8 aut.); Unité de Génétique Moléculaire des Virus Respiratoires, Institut Pasteur, 25 rue du Dr Roux/Paris/France (9 aut., 10 aut., 11 aut.); Department of Microbiology, The University of Hong Kong/Hong-Kong (12 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Journal of general virology; ISSN 0022-1317; Coden JGVIAY; Royaume-Uni; Da. 2005; Vol. 86; No. p.5; Pp. 1423-1434; Bibl. 2 p.1/4</SO>
<LA>Anglais</LA>
<EA>Post-translational modifications and correct subcellular localization of viral structural proteins are prerequisites for assembly and budding of enveloped viruses. Coronaviruses, like the severe acute respiratory syndrome-associated virus (SARS-CoV), bud from the endoplasmic reticulum-Golgi intermediate compartment. In this study, the subcellular distribution and maturation of SARS-CoV surface proteins S, M and E were analysed by using C-terminally tagged proteins. As early as 30 min post-entry into the endoplasmic reticulum, high-mannosylated S assembles into trimers prior to acquisition of complex N-glycans in the Golgi. Like S, M acquires high-mannose N-glycans that are subsequently modified into complex N-glycans in the Golgi. The N-glycosylation profile and the absence of O-glycosylation on M protein relate SARS-CoV to the previously described group 1 and 3 coronaviruses. Immunofluorescence analysis shows that S is detected in several compartments along the secretory pathway from the endoplasmic reticulum to the plasma membrane while M predominantly localizes in the Golgi, where it accumulates, and in trafficking vesicles. The E protein is not glycosylated. Pulse-chase labelling and confocal microscopy in the presence of protein translation inhibitor cycloheximide revealed that the E protein has a short half-life of 30 min. E protein is found in bright perinuclear patches colocalizing with endoplasmic reticulum markers. In conclusion, SARS-CoV surface proteins S, M and E show differential subcellular localizations when expressed alone suggesting that additional cellular or viral factors might be required for coordinated trafficking to the virus assembly site in the endoplasmic reticulum-Golgi intermediate compartment.</EA>
<CC>002A05C10</CC>
<FD>Coronavirus; Localisation; Protéine; Microbiologie; Virologie; Syndrome respiratoire aigu sévère</FD>
<FG>Coronaviridae; Nidovirales; Virus; Appareil respiratoire pathologie; Virose; Infection; Poumon pathologie</FG>
<ED>Coronavirus; Localization; Protein; Microbiology; Virology; Severe acute respiratory syndrome</ED>
<EG>Coronaviridae; Nidovirales; Virus; Respiratory disease; Viral disease; Infection; Lung disease</EG>
<SD>Coronavirus; Localización; Proteína; Microbiología; Virología; Síndrome respiratorio agudo severo</SD>
<LO>INIST-13533.354000129444440210</LO>
<ID>05-0229117</ID>
</server>
</inist>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/PascalFrancis/Corpus

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000687 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/PascalFrancis/Corpus/biblio.hfd -nk 000687 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    SrasV1
   |flux=    PascalFrancis
   |étape=   Corpus
   |type=    RBID
   |clé=     Pascal:05-0229117
   |texte=   Differential maturation and subcellular localization of severe acute respiratory syndrome coronavirus surface proteins S, M and E
}}

This area was generated with Dilib version V0.6.33.
Data generation: Tue Apr 28 14:49:16 2020. Site generation: Sat Mar 27 22:06:49 2021

	Serveur d'exploration SRAS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration SRAS

Differential maturation and subcellular localization of severe acute respiratory syndrome coronavirus surface proteins S, M and E

Differential maturation and subcellular localization of severe acute respiratory syndrome coronavirus surface proteins S, M and E

Source :

Descripteurs français

English descriptors

Abstract

Notice en format standard (ISO 2709)

Format Inist (serveur)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri