SrasV1, PascalFrancis, Corpus, bibRecord, 000645

Differential sensitivities of severe acute respiratory syndrome (SARS) coronavirus spike polypeptide enzyme-linked immunosorbent assay (ELISA) and SARS coronavirus nucleocapsid protein ELISA for serodiagnosis of SARS coronavirus pneumonia

Identifieur interne : 000645 ( PascalFrancis/Corpus ); précédent : 000644; suivant : 000646

Differential sensitivities of severe acute respiratory syndrome (SARS) coronavirus spike polypeptide enzyme-linked immunosorbent assay (ELISA) and SARS coronavirus nucleocapsid protein ELISA for serodiagnosis of SARS coronavirus pneumonia

Auteurs : Patrick C. Y. Woo ; Susanna K. P. Lau ; Beatrice H. L. Wong ; Hoi-Wah Tsoi ; Ami M. Y. Fung ; Richard Y. T. Kao ; Kwok-Hung Chan ; J. S. Malik Peiris ; Kwok-Yung Yuen

Source :

Journal of clinical microbiology : (Print) [ 0095-1137 ] ; 2005.

RBID : Pascal:05-0322075

Descripteurs français

Pascal (Inist)
- Virus syndrome respiratoire aigu sévère, Sensibilité, Polypeptide, Technique ELISA, Nucléocapside, Protéine, Microbiologie, Syndrome respiratoire aigu sévère, Pneumonie.

English descriptors

KwdEn :
- ELISA assay, Microbiology, Nucleocapsid, Pneumonia, Polypeptide, Protein, Sensitivity, Severe acute respiratory syndrome, Severe acute respiratory syndrome virus.

Abstract

The use of recombinant severe acute respiratory syndrome-coronavirus (SARS-CoV) nucleocapsid protein (N) enzyme-linked immunosorbent assay (ELISA)-based antibody and antigen tests for diagnosis of SARS-CoV infections have been widely reported. However, no recombinant SARS-CoV spike protein (S)-based ELISA is currently available. In this article, we describe the problems and solutions of setting up the recombinant SARS-CoV S-based ELISA for antibody detection. The SARS-CoV S-based immunoglobulin M (IgM) and IgG ELISAs were evaluated and compared with the corresponding N-based ELISA for serodiagnosis of SARS-CoV pneumonia, using sera from 148 healthy blood donors who donated blood 3 years ago as controls and 95 SARS-CoV pneumonia patients in Hong Kong. Results obtained by the recombinant S (rS)-based IgG ELISA using the regenerated S prepared by dialysis with decreasing concentrations of urea or direct addition of different coating buffers, followed by addition of different regeneration buffer, identified 4 M urea and 1 M sarcosine for plate coating and no regeneration buffer as the most optimal conditions for antibody detection. The specificities of the S-based ELISA for IgG and IgM detection were 98.6% and 93.9%, with corresponding sensitivities of 58.9% and 74.7%, respectively. The sensitivity of the rN IgG ELISA (94.7%) is significantly higher than that of the rS IgG ELISA (P < 0.001), whereas the sensitivity of the rS IgM ELISA is significantly higher than that of the rN IgM ELISA (55.2%) (P < 0.01). An ELISA for detection of IgM against S and N could be more sensitive than one that detects IgM against N alone for serodiagnosis of SARS-CoV pneumonia.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

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C01	`01`		`ENG`	@0 The use of recombinant severe acute respiratory syndrome-coronavirus (SARS-CoV) nucleocapsid protein (N) enzyme-linked immunosorbent assay (ELISA)-based antibody and antigen tests for diagnosis of SARS-CoV infections have been widely reported. However, no recombinant SARS-CoV spike protein (S)-based ELISA is currently available. In this article, we describe the problems and solutions of setting up the recombinant SARS-CoV S-based ELISA for antibody detection. The SARS-CoV S-based immunoglobulin M (IgM) and IgG ELISAs were evaluated and compared with the corresponding N-based ELISA for serodiagnosis of SARS-CoV pneumonia, using sera from 148 healthy blood donors who donated blood 3 years ago as controls and 95 SARS-CoV pneumonia patients in Hong Kong. Results obtained by the recombinant S (rS)-based IgG ELISA using the regenerated S prepared by dialysis with decreasing concentrations of urea or direct addition of different coating buffers, followed by addition of different regeneration buffer, identified 4 M urea and 1 M sarcosine for plate coating and no regeneration buffer as the most optimal conditions for antibody detection. The specificities of the S-based ELISA for IgG and IgM detection were 98.6% and 93.9%, with corresponding sensitivities of 58.9% and 74.7%, respectively. The sensitivity of the rN IgG ELISA (94.7%) is significantly higher than that of the rS IgG ELISA (P < 0.001), whereas the sensitivity of the rS IgM ELISA is significantly higher than that of the rN IgM ELISA (55.2%) (P < 0.01). An ELISA for detection of IgM against S and N could be more sensitive than one that detects IgM against N alone for serodiagnosis of SARS-CoV pneumonia.
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Format Inist (serveur)

NO :	PASCAL 05-0322075 INIST
ET :	Differential sensitivities of severe acute respiratory syndrome (SARS) coronavirus spike polypeptide enzyme-linked immunosorbent assay (ELISA) and SARS coronavirus nucleocapsid protein ELISA for serodiagnosis of SARS coronavirus pneumonia
AU :	WOO (Patrick C. Y.); LAU (Susanna K. P.); WONG (Beatrice H. L.); TSOI (Hoi-Wah); FUNG (Ami M. Y.); KAO (Richard Y. T.); CHAN (Kwok-Hung); MALIK PEIRIS (J. S.); YUEN (Kwok-Yung)
AF :	Department of Microbiology, Faculty of Medicine, The University of Hong Kong/Hong-Kong (1 aut., 2 aut., 3 aut., 4 aut., 5 aut., 6 aut., 7 aut., 8 aut., 9 aut.); Research Centre of Infection and Immunology, Faculty of Medicine, The University of Hong Kong/Hong-Kong (1 aut., 2 aut., 6 aut., 8 aut., 9 aut.)
DT :	Publication en série; Niveau analytique
SO :	Journal of clinical microbiology : (Print); ISSN 0095-1137; Coden JCMIDW; Etats-Unis; Da. 2005; Vol. 43; No. 7; Pp. 3054-3058; Bibl. 27 ref.
LA :	Anglais
EA :	The use of recombinant severe acute respiratory syndrome-coronavirus (SARS-CoV) nucleocapsid protein (N) enzyme-linked immunosorbent assay (ELISA)-based antibody and antigen tests for diagnosis of SARS-CoV infections have been widely reported. However, no recombinant SARS-CoV spike protein (S)-based ELISA is currently available. In this article, we describe the problems and solutions of setting up the recombinant SARS-CoV S-based ELISA for antibody detection. The SARS-CoV S-based immunoglobulin M (IgM) and IgG ELISAs were evaluated and compared with the corresponding N-based ELISA for serodiagnosis of SARS-CoV pneumonia, using sera from 148 healthy blood donors who donated blood 3 years ago as controls and 95 SARS-CoV pneumonia patients in Hong Kong. Results obtained by the recombinant S (rS)-based IgG ELISA using the regenerated S prepared by dialysis with decreasing concentrations of urea or direct addition of different coating buffers, followed by addition of different regeneration buffer, identified 4 M urea and 1 M sarcosine for plate coating and no regeneration buffer as the most optimal conditions for antibody detection. The specificities of the S-based ELISA for IgG and IgM detection were 98.6% and 93.9%, with corresponding sensitivities of 58.9% and 74.7%, respectively. The sensitivity of the rN IgG ELISA (94.7%) is significantly higher than that of the rS IgG ELISA (P < 0.001), whereas the sensitivity of the rS IgM ELISA is significantly higher than that of the rN IgM ELISA (55.2%) (P < 0.01). An ELISA for detection of IgM against S and N could be more sensitive than one that detects IgM against N alone for serodiagnosis of SARS-CoV pneumonia.
CC :	002A05; 002B05
FD :	Virus syndrome respiratoire aigu sévère; Sensibilité; Polypeptide; Technique ELISA; Nucléocapside; Protéine; Microbiologie; Syndrome respiratoire aigu sévère; Pneumonie
FG :	Coronavirus; Coronaviridae; Nidovirales; Virus; Appareil respiratoire pathologie; Virose; Infection; Poumon pathologie
ED :	Severe acute respiratory syndrome virus; Sensitivity; Polypeptide; ELISA assay; Nucleocapsid; Protein; Microbiology; Severe acute respiratory syndrome; Pneumonia
EG :	Coronavirus; Coronaviridae; Nidovirales; Virus; Respiratory disease; Viral disease; Infection; Lung disease
SD :	Severe acute respiratory syndrome virus; Sensibilidad; Polipéptido; Técnica ELISA; Nucleocápside; Proteína; Microbiología; Síndrome respiratorio agudo severo; Neumonía
LO :	INIST-17088.354000132226670050
ID :	05-0322075

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Pascal:05-0322075

Le document en format XML

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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Differential sensitivities of severe acute respiratory syndrome (SARS) coronavirus spike polypeptide enzyme-linked immunosorbent assay (ELISA) and SARS coronavirus nucleocapsid protein ELISA for serodiagnosis of SARS coronavirus pneumonia</title>
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<author><name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok-Hung" last="Chan">Kwok-Hung Chan</name>
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<author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name>
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<series><title level="j" type="main">Journal of clinical microbiology : (Print)</title>
<title level="j" type="abbreviated">J. clin. microbiol. : (Print)</title>
<idno type="ISSN">0095-1137</idno>
<imprint><date when="2005">2005</date>
</imprint>
</series>
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<seriesStmt><title level="j" type="main">Journal of clinical microbiology : (Print)</title>
<title level="j" type="abbreviated">J. clin. microbiol. : (Print)</title>
<idno type="ISSN">0095-1137</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>ELISA assay</term>
<term>Microbiology</term>
<term>Nucleocapsid</term>
<term>Pneumonia</term>
<term>Polypeptide</term>
<term>Protein</term>
<term>Sensitivity</term>
<term>Severe acute respiratory syndrome</term>
<term>Severe acute respiratory syndrome virus</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Virus syndrome respiratoire aigu sévère</term>
<term>Sensibilité</term>
<term>Polypeptide</term>
<term>Technique ELISA</term>
<term>Nucléocapside</term>
<term>Protéine</term>
<term>Microbiologie</term>
<term>Syndrome respiratoire aigu sévère</term>
<term>Pneumonie</term>
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<front><div type="abstract" xml:lang="en">The use of recombinant severe acute respiratory syndrome-coronavirus (SARS-CoV) nucleocapsid protein (N) enzyme-linked immunosorbent assay (ELISA)-based antibody and antigen tests for diagnosis of SARS-CoV infections have been widely reported. However, no recombinant SARS-CoV spike protein (S)-based ELISA is currently available. In this article, we describe the problems and solutions of setting up the recombinant SARS-CoV S-based ELISA for antibody detection. The SARS-CoV S-based immunoglobulin M (IgM) and IgG ELISAs were evaluated and compared with the corresponding N-based ELISA for serodiagnosis of SARS-CoV pneumonia, using sera from 148 healthy blood donors who donated blood 3 years ago as controls and 95 SARS-CoV pneumonia patients in Hong Kong. Results obtained by the recombinant S (rS)-based IgG ELISA using the regenerated S prepared by dialysis with decreasing concentrations of urea or direct addition of different coating buffers, followed by addition of different regeneration buffer, identified 4 M urea and 1 M sarcosine for plate coating and no regeneration buffer as the most optimal conditions for antibody detection. The specificities of the S-based ELISA for IgG and IgM detection were 98.6% and 93.9%, with corresponding sensitivities of 58.9% and 74.7%, respectively. The sensitivity of the rN IgG ELISA (94.7%) is significantly higher than that of the rS IgG ELISA (P < 0.001), whereas the sensitivity of the rS IgM ELISA is significantly higher than that of the rN IgM ELISA (55.2%) (P < 0.01). An ELISA for detection of IgM against S and N could be more sensitive than one that detects IgM against N alone for serodiagnosis of SARS-CoV pneumonia.</div>
</front>
</TEI>
<inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0095-1137</s0>
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<fA03 i2="1"><s0>J. clin. microbiol. : (Print)</s0>
</fA03>
<fA05><s2>43</s2>
</fA05>
<fA06><s2>7</s2>
</fA06>
<fA08 i1="01" i2="1" l="ENG"><s1>Differential sensitivities of severe acute respiratory syndrome (SARS) coronavirus spike polypeptide enzyme-linked immunosorbent assay (ELISA) and SARS coronavirus nucleocapsid protein ELISA for serodiagnosis of SARS coronavirus pneumonia</s1>
</fA08>
<fA11 i1="01" i2="1"><s1>WOO (Patrick C. Y.)</s1>
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<fA11 i1="02" i2="1"><s1>LAU (Susanna K. P.)</s1>
</fA11>
<fA11 i1="03" i2="1"><s1>WONG (Beatrice H. L.)</s1>
</fA11>
<fA11 i1="04" i2="1"><s1>TSOI (Hoi-Wah)</s1>
</fA11>
<fA11 i1="05" i2="1"><s1>FUNG (Ami M. Y.)</s1>
</fA11>
<fA11 i1="06" i2="1"><s1>KAO (Richard Y. T.)</s1>
</fA11>
<fA11 i1="07" i2="1"><s1>CHAN (Kwok-Hung)</s1>
</fA11>
<fA11 i1="08" i2="1"><s1>MALIK PEIRIS (J. S.)</s1>
</fA11>
<fA11 i1="09" i2="1"><s1>YUEN (Kwok-Yung)</s1>
</fA11>
<fA14 i1="01"><s1>Department of Microbiology, Faculty of Medicine, The University of Hong Kong</s1>
<s3>HKG</s3>
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<fA14 i1="02"><s1>Research Centre of Infection and Immunology, Faculty of Medicine, The University of Hong Kong</s1>
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<fA20><s1>3054-3058</s1>
</fA20>
<fA21><s1>2005</s1>
</fA21>
<fA23 i1="01"><s0>ENG</s0>
</fA23>
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<s2>17088</s2>
<s5>354000132226670050</s5>
</fA43>
<fA44><s0>0000</s0>
<s1>© 2005 INIST-CNRS. All rights reserved.</s1>
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<fA45><s0>27 ref.</s0>
</fA45>
<fA47 i1="01" i2="1"><s0>05-0322075</s0>
</fA47>
<fA60><s1>P</s1>
</fA60>
<fA61><s0>A</s0>
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<fA64 i1="01" i2="1"><s0>Journal of clinical microbiology : (Print)</s0>
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<fA66 i1="01"><s0>USA</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>The use of recombinant severe acute respiratory syndrome-coronavirus (SARS-CoV) nucleocapsid protein (N) enzyme-linked immunosorbent assay (ELISA)-based antibody and antigen tests for diagnosis of SARS-CoV infections have been widely reported. However, no recombinant SARS-CoV spike protein (S)-based ELISA is currently available. In this article, we describe the problems and solutions of setting up the recombinant SARS-CoV S-based ELISA for antibody detection. The SARS-CoV S-based immunoglobulin M (IgM) and IgG ELISAs were evaluated and compared with the corresponding N-based ELISA for serodiagnosis of SARS-CoV pneumonia, using sera from 148 healthy blood donors who donated blood 3 years ago as controls and 95 SARS-CoV pneumonia patients in Hong Kong. Results obtained by the recombinant S (rS)-based IgG ELISA using the regenerated S prepared by dialysis with decreasing concentrations of urea or direct addition of different coating buffers, followed by addition of different regeneration buffer, identified 4 M urea and 1 M sarcosine for plate coating and no regeneration buffer as the most optimal conditions for antibody detection. The specificities of the S-based ELISA for IgG and IgM detection were 98.6% and 93.9%, with corresponding sensitivities of 58.9% and 74.7%, respectively. The sensitivity of the rN IgG ELISA (94.7%) is significantly higher than that of the rS IgG ELISA (P < 0.001), whereas the sensitivity of the rS IgM ELISA is significantly higher than that of the rN IgM ELISA (55.2%) (P < 0.01). An ELISA for detection of IgM against S and N could be more sensitive than one that detects IgM against N alone for serodiagnosis of SARS-CoV pneumonia.</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>002A05</s0>
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<fC02 i1="02" i2="X"><s0>002B05</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Virus syndrome respiratoire aigu sévère</s0>
<s2>NW</s2>
<s5>01</s5>
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<s2>NW</s2>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Severe acute respiratory syndrome virus</s0>
<s2>NW</s2>
<s5>01</s5>
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<s5>06</s5>
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<fC03 i1="03" i2="X" l="ENG"><s0>Polypeptide</s0>
<s5>06</s5>
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<fC03 i1="03" i2="X" l="SPA"><s0>Polipéptido</s0>
<s5>06</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Technique ELISA</s0>
<s5>07</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>ELISA assay</s0>
<s5>07</s5>
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<fC03 i1="04" i2="X" l="SPA"><s0>Técnica ELISA</s0>
<s5>07</s5>
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<s5>08</s5>
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<fC03 i1="05" i2="X" l="ENG"><s0>Nucleocapsid</s0>
<s5>08</s5>
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<s5>08</s5>
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<s5>09</s5>
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<s5>09</s5>
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<fC03 i1="06" i2="X" l="SPA"><s0>Proteína</s0>
<s5>09</s5>
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<s5>10</s5>
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<s5>10</s5>
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<fC03 i1="08" i2="X" l="FRE"><s0>Syndrome respiratoire aigu sévère</s0>
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<s5>14</s5>
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<s2>NM</s2>
<s5>14</s5>
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<fC03 i1="08" i2="X" l="SPA"><s0>Síndrome respiratorio agudo severo</s0>
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<s5>14</s5>
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<s5>15</s5>
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<s5>15</s5>
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<s5>15</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Coronavirus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Coronavirus</s0>
<s2>NW</s2>
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<fC07 i1="01" i2="X" l="SPA"><s0>Coronavirus</s0>
<s2>NW</s2>
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<s2>NW</s2>
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<fC07 i1="02" i2="X" l="ENG"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Coronaviridae</s0>
<s2>NW</s2>
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<s2>NW</s2>
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<s2>NW</s2>
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<s2>NW</s2>
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<s2>NW</s2>
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<s5>13</s5>
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<fC07 i1="06" i2="X" l="FRE"><s0>Virose</s0>
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<fC07 i1="06" i2="X" l="ENG"><s0>Viral disease</s0>
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<s5>16</s5>
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<fC07 i1="08" i2="X" l="SPA"><s0>Pulmón patología</s0>
<s5>16</s5>
</fC07>
<fN21><s1>227</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
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<server><NO>PASCAL 05-0322075 INIST</NO>
<ET>Differential sensitivities of severe acute respiratory syndrome (SARS) coronavirus spike polypeptide enzyme-linked immunosorbent assay (ELISA) and SARS coronavirus nucleocapsid protein ELISA for serodiagnosis of SARS coronavirus pneumonia</ET>
<AU>WOO (Patrick C. Y.); LAU (Susanna K. P.); WONG (Beatrice H. L.); TSOI (Hoi-Wah); FUNG (Ami M. Y.); KAO (Richard Y. T.); CHAN (Kwok-Hung); MALIK PEIRIS (J. S.); YUEN (Kwok-Yung)</AU>
<AF>Department of Microbiology, Faculty of Medicine, The University of Hong Kong/Hong-Kong (1 aut., 2 aut., 3 aut., 4 aut., 5 aut., 6 aut., 7 aut., 8 aut., 9 aut.); Research Centre of Infection and Immunology, Faculty of Medicine, The University of Hong Kong/Hong-Kong (1 aut., 2 aut., 6 aut., 8 aut., 9 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Journal of clinical microbiology : (Print); ISSN 0095-1137; Coden JCMIDW; Etats-Unis; Da. 2005; Vol. 43; No. 7; Pp. 3054-3058; Bibl. 27 ref.</SO>
<LA>Anglais</LA>
<EA>The use of recombinant severe acute respiratory syndrome-coronavirus (SARS-CoV) nucleocapsid protein (N) enzyme-linked immunosorbent assay (ELISA)-based antibody and antigen tests for diagnosis of SARS-CoV infections have been widely reported. However, no recombinant SARS-CoV spike protein (S)-based ELISA is currently available. In this article, we describe the problems and solutions of setting up the recombinant SARS-CoV S-based ELISA for antibody detection. The SARS-CoV S-based immunoglobulin M (IgM) and IgG ELISAs were evaluated and compared with the corresponding N-based ELISA for serodiagnosis of SARS-CoV pneumonia, using sera from 148 healthy blood donors who donated blood 3 years ago as controls and 95 SARS-CoV pneumonia patients in Hong Kong. Results obtained by the recombinant S (rS)-based IgG ELISA using the regenerated S prepared by dialysis with decreasing concentrations of urea or direct addition of different coating buffers, followed by addition of different regeneration buffer, identified 4 M urea and 1 M sarcosine for plate coating and no regeneration buffer as the most optimal conditions for antibody detection. The specificities of the S-based ELISA for IgG and IgM detection were 98.6% and 93.9%, with corresponding sensitivities of 58.9% and 74.7%, respectively. The sensitivity of the rN IgG ELISA (94.7%) is significantly higher than that of the rS IgG ELISA (P < 0.001), whereas the sensitivity of the rS IgM ELISA is significantly higher than that of the rN IgM ELISA (55.2%) (P < 0.01). An ELISA for detection of IgM against S and N could be more sensitive than one that detects IgM against N alone for serodiagnosis of SARS-CoV pneumonia.</EA>
<CC>002A05; 002B05</CC>
<FD>Virus syndrome respiratoire aigu sévère; Sensibilité; Polypeptide; Technique ELISA; Nucléocapside; Protéine; Microbiologie; Syndrome respiratoire aigu sévère; Pneumonie</FD>
<FG>Coronavirus; Coronaviridae; Nidovirales; Virus; Appareil respiratoire pathologie; Virose; Infection; Poumon pathologie</FG>
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   |texte=   Differential sensitivities of severe acute respiratory syndrome (SARS) coronavirus spike polypeptide enzyme-linked immunosorbent assay (ELISA) and SARS coronavirus nucleocapsid protein ELISA for serodiagnosis of SARS coronavirus pneumonia
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Differential sensitivities of severe acute respiratory syndrome (SARS) coronavirus spike polypeptide enzyme-linked immunosorbent assay (ELISA) and SARS coronavirus nucleocapsid protein ELISA for serodiagnosis of SARS coronavirus pneumonia

Differential sensitivities of severe acute respiratory syndrome (SARS) coronavirus spike polypeptide enzyme-linked immunosorbent assay (ELISA) and SARS coronavirus nucleocapsid protein ELISA for serodiagnosis of SARS coronavirus pneumonia

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