Evaluation of a peptide-based enzyme immunoassay for anti-SARS coronavirus IgG antibody
Identifieur interne : 000616 ( PascalFrancis/Corpus ); précédent : 000615; suivant : 000617Evaluation of a peptide-based enzyme immunoassay for anti-SARS coronavirus IgG antibody
Auteurs : Paul K. S. Chan ; W. K. To ; Esther Y. M. Liu ; T. K. Ng ; John S. Tam ; Joseph J. Y. Sung ; Jean-Michel Lacroix ; Michel HoudeSource :
- Journal of medical virology [ 0146-6615 ] ; 2004.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
High throughput assays for anti-SARS-CoV IgG antibody detection are need for large-scale epidemiologic studies. The performance of a microplate enzyme immunoassay, DETECT-SARS, was evaluated for the detection of anti-SARS-CoV IgG antibody. This assay is based on synthetic peptides derived from the nucleocapsid and spike proteins. The results showed that the assay provided a high degree of sensitivity (95.9%) for convalescent serum samples. The level of specificity was close to 90%, and did not show significant variation among different control groups. The high degree of sensitivity together with the high-throughput nature makes it advantageous as a screening assay for studies where handling of a large number of specimens is required.
Notice en format standard (ISO 2709)
Pour connaître la documentation sur le format Inist Standard.
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Format Inist (serveur)
NO : | PASCAL 05-0395225 INIST |
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ET : | Evaluation of a peptide-based enzyme immunoassay for anti-SARS coronavirus IgG antibody |
AU : | CHAN (Paul K. S.); TO (W. K.); LIU (Esther Y. M.); NG (T. K.); TAM (John S.); SUNG (Joseph J. Y.); LACROIX (Jean-Michel); HOUDE (Michel) |
AF : | Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Prince of Wales Hospital/Shatin, New Territories/Hong-Kong (1 aut., 5 aut., 6 aut.); Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital/Shatin, New Territories/Hong-Kong (1 aut., 3 aut., 5 aut.); Department of Pathology, Princess Margaret Hospital/Kowloon, Shatin, New Territories/Hong-Kong (2 aut., 4 aut.); Adaltis Development, Inc./Montreal, Quebec/Canada (7 aut., 8 aut.) |
DT : | Publication en série; Niveau analytique |
SO : | Journal of medical virology; ISSN 0146-6615; Coden JMVIDB; Etats-Unis; Da. 2004; Vol. 74; No. 4; Pp. 517-520; Bibl. 17 ref. |
LA : | Anglais |
EA : | High throughput assays for anti-SARS-CoV IgG antibody detection are need for large-scale epidemiologic studies. The performance of a microplate enzyme immunoassay, DETECT-SARS, was evaluated for the detection of anti-SARS-CoV IgG antibody. This assay is based on synthetic peptides derived from the nucleocapsid and spike proteins. The results showed that the assay provided a high degree of sensitivity (95.9%) for convalescent serum samples. The level of specificity was close to 90%, and did not show significant variation among different control groups. The high degree of sensitivity together with the high-throughput nature makes it advantageous as a screening assay for studies where handling of a large number of specimens is required. |
CC : | 002A05C10; 002B05C02J; 002A05C08 |
FD : | Virus syndrome respiratoire aigu sévère; Peptide; Méthode immunoenzymatique; IgG; Anticorps; Sérologie; Syndrome respiratoire aigu sévère |
FG : | Coronavirus; Coronaviridae; Nidovirales; Virus; Appareil respiratoire pathologie; Virose; Infection; Poumon pathologie |
ED : | Severe acute respiratory syndrome virus; Peptides; Enzyme immunoassay; IgG; Antibody; Serology; Severe acute respiratory syndrome |
EG : | Coronavirus; Coronaviridae; Nidovirales; Virus; Respiratory disease; Viral disease; Infection; Lung disease |
SD : | Severe acute respiratory syndrome virus; Péptido; Método inmunoenzimático; IgG; Anticuerpo; Serología; Síndrome respiratorio agudo severo |
LO : | INIST-17422.354000122446470010 |
ID : | 05-0395225 |
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Pascal:05-0395225Le document en format XML
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<front><div type="abstract" xml:lang="en">High throughput assays for anti-SARS-CoV IgG antibody detection are need for large-scale epidemiologic studies. The performance of a microplate enzyme immunoassay, DETECT-SARS, was evaluated for the detection of anti-SARS-CoV IgG antibody. This assay is based on synthetic peptides derived from the nucleocapsid and spike proteins. The results showed that the assay provided a high degree of sensitivity (95.9%) for convalescent serum samples. The level of specificity was close to 90%, and did not show significant variation among different control groups. The high degree of sensitivity together with the high-throughput nature makes it advantageous as a screening assay for studies where handling of a large number of specimens is required.</div>
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<fC07 i1="03" i2="X" l="SPA"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Appareil respiratoire pathologie</s0>
<s5>13</s5>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Respiratory disease</s0>
<s5>13</s5>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Aparato respiratorio patología</s0>
<s5>13</s5>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Virose</s0>
</fC07>
<fC07 i1="06" i2="X" l="ENG"><s0>Viral disease</s0>
</fC07>
<fC07 i1="06" i2="X" l="SPA"><s0>Virosis</s0>
</fC07>
<fC07 i1="07" i2="X" l="FRE"><s0>Infection</s0>
</fC07>
<fC07 i1="07" i2="X" l="ENG"><s0>Infection</s0>
</fC07>
<fC07 i1="07" i2="X" l="SPA"><s0>Infección</s0>
</fC07>
<fC07 i1="08" i2="X" l="FRE"><s0>Poumon pathologie</s0>
<s5>16</s5>
</fC07>
<fC07 i1="08" i2="X" l="ENG"><s0>Lung disease</s0>
<s5>16</s5>
</fC07>
<fC07 i1="08" i2="X" l="SPA"><s0>Pulmón patología</s0>
<s5>16</s5>
</fC07>
<fN21><s1>276</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
</standard>
<server><NO>PASCAL 05-0395225 INIST</NO>
<ET>Evaluation of a peptide-based enzyme immunoassay for anti-SARS coronavirus IgG antibody</ET>
<AU>CHAN (Paul K. S.); TO (W. K.); LIU (Esther Y. M.); NG (T. K.); TAM (John S.); SUNG (Joseph J. Y.); LACROIX (Jean-Michel); HOUDE (Michel)</AU>
<AF>Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Prince of Wales Hospital/Shatin, New Territories/Hong-Kong (1 aut., 5 aut., 6 aut.); Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital/Shatin, New Territories/Hong-Kong (1 aut., 3 aut., 5 aut.); Department of Pathology, Princess Margaret Hospital/Kowloon, Shatin, New Territories/Hong-Kong (2 aut., 4 aut.); Adaltis Development, Inc./Montreal, Quebec/Canada (7 aut., 8 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Journal of medical virology; ISSN 0146-6615; Coden JMVIDB; Etats-Unis; Da. 2004; Vol. 74; No. 4; Pp. 517-520; Bibl. 17 ref.</SO>
<LA>Anglais</LA>
<EA>High throughput assays for anti-SARS-CoV IgG antibody detection are need for large-scale epidemiologic studies. The performance of a microplate enzyme immunoassay, DETECT-SARS, was evaluated for the detection of anti-SARS-CoV IgG antibody. This assay is based on synthetic peptides derived from the nucleocapsid and spike proteins. The results showed that the assay provided a high degree of sensitivity (95.9%) for convalescent serum samples. The level of specificity was close to 90%, and did not show significant variation among different control groups. The high degree of sensitivity together with the high-throughput nature makes it advantageous as a screening assay for studies where handling of a large number of specimens is required.</EA>
<CC>002A05C10; 002B05C02J; 002A05C08</CC>
<FD>Virus syndrome respiratoire aigu sévère; Peptide; Méthode immunoenzymatique; IgG; Anticorps; Sérologie; Syndrome respiratoire aigu sévère</FD>
<FG>Coronavirus; Coronaviridae; Nidovirales; Virus; Appareil respiratoire pathologie; Virose; Infection; Poumon pathologie</FG>
<ED>Severe acute respiratory syndrome virus; Peptides; Enzyme immunoassay; IgG; Antibody; Serology; Severe acute respiratory syndrome</ED>
<EG>Coronavirus; Coronaviridae; Nidovirales; Virus; Respiratory disease; Viral disease; Infection; Lung disease</EG>
<SD>Severe acute respiratory syndrome virus; Péptido; Método inmunoenzimático; IgG; Anticuerpo; Serología; Síndrome respiratorio agudo severo</SD>
<LO>INIST-17422.354000122446470010</LO>
<ID>05-0395225</ID>
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