SrasV1, PascalFrancis, Corpus, bibRecord, 000587

Anti-SARS coronavirus 3C-like protease effects of Isatis indigotica root and plant-derived phenolic compounds

Identifieur interne : 000587 ( PascalFrancis/Corpus ); précédent : 000586; suivant : 000588

Anti-SARS coronavirus 3C-like protease effects of Isatis indigotica root and plant-derived phenolic compounds

Auteurs : Cheng-Wen Lin ; Fuu-Jen Tsai ; Chang-Hai Tsai ; Chien-Chen Lai ; LEI WAN ; Tin-Yun Ho ; Chang-Chi Hsieh ; Pei-Dawn Lee Chao

Source :

Antiviral research [ 0166-3542 ] ; 2005.

RBID : Pascal:05-0472143

Descripteurs français

Pascal (Inist)
- Virus syndrome respiratoire aigu sévère, Peptidases, Racine, Antiviral, Origine végétale, Cruciferae, Phénols, Isatis indigotica.

English descriptors

KwdEn :
- Antiviral, Cruciferae, Peptidases, Phenols, Plant origin, Root, Severe acute respiratory syndrome virus.

Abstract

The 3C-like protease (3CL^pro) of SARS-coronavirus mediates the proteolytic processing of replicase polypeptides 1a and 1ab into functional proteins, becoming an important target for the drug development. In this study, Isatis indigotica root extract, five major compounds of I. indigotica root, and seven plant-derived phenolic compounds were tested for anti-SARS-CoV 3CL^pro effects using cell-free and cell-based cleavage assays. Cleavage assays with the 3CL^pro demonstrated that IC₅₀ values were in micromolar ranges for I. indigotica root extract, indigo, sinigrin, aloe emodin and hesperetin. Sinigrin (IC₅₀: 217 μM) was more efficient in blocking the cleavage processing of the 3CL^pro than indigo (IC₅₀: 752 μM) and beta-sitosterol (IC₅₀: 1210 μM) in the cell-based assay. Only two phenolic compounds aloe emodin and hesperetin dose-dependently inhibited cleavage activity of the 3CL^pro, in which the IC₅₀ was 366 μM for aloe emodin and 8.3 μM for hesperetin in the cell-based assay.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

A01	`01`	`1`		`@0 0166-3542`
A02	`01`			`@0 ARSRDR`
A03		`1`		`@0 Antivir. res.`
A05				`@2 68`
A06				`@2 1`
A08	`01`	`1`	`ENG`	`@1 Anti-SARS coronavirus 3C-like protease effects of Isatis indigotica root and plant-derived phenolic compounds`
A11	`01`	`1`		`@1 LIN (Cheng-Wen)`
A11	`02`	`1`		`@1 TSAI (Fuu-Jen)`
A11	`03`	`1`		`@1 TSAI (Chang-Hai)`
A11	`04`	`1`		`@1 LAI (Chien-Chen)`
A11	`05`	`1`		`@1 LEI WAN`
A11	`06`	`1`		`@1 HO (Tin-Yun)`
A11	`07`	`1`		`@1 HSIEH (Chang-Chi)`
A11	`08`	`1`		`@1 CHAO (Pei-Dawn Lee)`
A14	`01`			`@1 Department of Medical Laboratory Science and Biotechnology, China Medical University, No. 91, Hsueh-Shih Road @2 Taichung 404 @3 TWN @Z 1 aut.`
A14	`02`			`@1 Clinical Virology Laboratory, Department of Laboratory Medicine, China Medical University Hospital @2 Taichung 404 @3 TWN @Z 1 aut.`
A14	`03`			`@1 Department of Medical Genetics and Medical Research, China Medical University Hospital @2 Taichung 404 @3 TWN @Z 2 aut. @Z 3 aut. @Z 4 aut. @Z 5 aut.`
A14	`04`			`@1 Institute of Chinese Medical Science, China Medical University @2 Taichung 404 @3 TWN @Z 6 aut.`
A14	`05`			`@1 Institute of Integration Chinese and Western Medicine, China Medical University @2 Taichung 404 @3 TWN @Z 7 aut.`
A14	`06`			`@1 Department of Pharmacy, China Medical University @2 Taichung 404 @3 TWN @Z 8 aut.`
A20				`@1 36-42`
A21				`@1 2005`
A23	`01`			`@0 ENG`
A43	`01`			`@1 INIST @2 18839 @5 354000132724070050`
A44				`@0 0000 @1 © 2005 INIST-CNRS. All rights reserved.`
A45				`@0 31 ref.`
A47	`01`	`1`		`@0 05-0472143`
A60				`@1 P`
A61				`@0 A`
A64	`01`	`1`		`@0 Antiviral research`
A66	`01`			`@0 NLD`
C01	`01`		`ENG`	@0 The 3C-like protease (3CL^pro) of SARS-coronavirus mediates the proteolytic processing of replicase polypeptides 1a and 1ab into functional proteins, becoming an important target for the drug development. In this study, Isatis indigotica root extract, five major compounds of I. indigotica root, and seven plant-derived phenolic compounds were tested for anti-SARS-CoV 3CL^pro effects using cell-free and cell-based cleavage assays. Cleavage assays with the 3CL^pro demonstrated that IC₅₀ values were in micromolar ranges for I. indigotica root extract, indigo, sinigrin, aloe emodin and hesperetin. Sinigrin (IC₅₀: 217 μM) was more efficient in blocking the cleavage processing of the 3CL^pro than indigo (IC₅₀: 752 μM) and beta-sitosterol (IC₅₀: 1210 μM) in the cell-based assay. Only two phenolic compounds aloe emodin and hesperetin dose-dependently inhibited cleavage activity of the 3CL^pro, in which the IC₅₀ was 366 μM for aloe emodin and 8.3 μM for hesperetin in the cell-based assay.
C02	`01`	`X`		`@0 002B02S05`
C02	`02`	`X`		`@0 002B02A04`
C03	`01`	`X`	`FRE`	`@0 Virus syndrome respiratoire aigu sévère @2 NW @5 01`
C03	`01`	`X`	`ENG`	`@0 Severe acute respiratory syndrome virus @2 NW @5 01`
C03	`01`	`X`	`SPA`	`@0 Severe acute respiratory syndrome virus @2 NW @5 01`
C03	`02`	`X`	`FRE`	`@0 Peptidases @2 FE @5 02`
C03	`02`	`X`	`ENG`	`@0 Peptidases @2 FE @5 02`
C03	`02`	`X`	`SPA`	`@0 Peptidases @2 FE @5 02`
C03	`03`	`X`	`FRE`	`@0 Racine @5 03`
C03	`03`	`X`	`ENG`	`@0 Root @5 03`
C03	`03`	`X`	`SPA`	`@0 Raíz @5 03`
C03	`04`	`X`	`FRE`	`@0 Antiviral @5 04`
C03	`04`	`X`	`ENG`	`@0 Antiviral @5 04`
C03	`04`	`X`	`SPA`	`@0 Antiviral @5 04`
C03	`05`	`X`	`FRE`	`@0 Origine végétale @5 05`
C03	`05`	`X`	`ENG`	`@0 Plant origin @5 05`
C03	`05`	`X`	`SPA`	`@0 Origen vegetal @5 05`
C03	`06`	`X`	`FRE`	`@0 Cruciferae @2 NS @5 06`
C03	`06`	`X`	`ENG`	`@0 Cruciferae @2 NS @5 06`
C03	`06`	`X`	`SPA`	`@0 Cruciferae @2 NS @5 06`
C03	`07`	`X`	`FRE`	`@0 Phénols @2 FX @5 07`
C03	`07`	`X`	`ENG`	`@0 Phenols @2 FX @5 07`
C03	`07`	`X`	`SPA`	`@0 Fenoles @2 FX @5 07`
C03	`08`	`X`	`FRE`	`@0 Isatis indigotica @4 INC @5 86`
C07	`01`	`X`	`FRE`	`@0 Coronavirus @2 NW`
C07	`01`	`X`	`ENG`	`@0 Coronavirus @2 NW`
C07	`01`	`X`	`SPA`	`@0 Coronavirus @2 NW`
C07	`02`	`X`	`FRE`	`@0 Coronaviridae @2 NW`
C07	`02`	`X`	`ENG`	`@0 Coronaviridae @2 NW`
C07	`02`	`X`	`SPA`	`@0 Coronaviridae @2 NW`
C07	`03`	`X`	`FRE`	`@0 Nidovirales @2 NW`
C07	`03`	`X`	`ENG`	`@0 Nidovirales @2 NW`
C07	`03`	`X`	`SPA`	`@0 Nidovirales @2 NW`
C07	`04`	`X`	`FRE`	`@0 Virus @2 NW`
C07	`04`	`X`	`ENG`	`@0 Virus @2 NW`
C07	`04`	`X`	`SPA`	`@0 Virus @2 NW`
C07	`05`	`X`	`FRE`	`@0 Hydrolases @2 FE`
C07	`05`	`X`	`ENG`	`@0 Hydrolases @2 FE`
C07	`05`	`X`	`SPA`	`@0 Hydrolases @2 FE`
C07	`06`	`X`	`FRE`	`@0 Enzyme @2 FE`
C07	`06`	`X`	`ENG`	`@0 Enzyme @2 FE`
C07	`06`	`X`	`SPA`	`@0 Enzima @2 FE`
C07	`07`	`X`	`FRE`	`@0 Dicotyledones @2 NS`
C07	`07`	`X`	`ENG`	`@0 Dicotyledones @2 NS`
C07	`07`	`X`	`SPA`	`@0 Dicotyledones @2 NS`
C07	`08`	`X`	`FRE`	`@0 Angiospermae @2 NS`
C07	`08`	`X`	`ENG`	`@0 Angiospermae @2 NS`
C07	`08`	`X`	`SPA`	`@0 Angiospermae @2 NS`
C07	`09`	`X`	`FRE`	`@0 Spermatophyta @2 NS`
C07	`09`	`X`	`ENG`	`@0 Spermatophyta @2 NS`
C07	`09`	`X`	`SPA`	`@0 Spermatophyta @2 NS`
N21				`@1 332`

Format Inist (serveur)

NO :	PASCAL 05-0472143 INIST
ET :	Anti-SARS coronavirus 3C-like protease effects of Isatis indigotica root and plant-derived phenolic compounds
AU :	LIN (Cheng-Wen); TSAI (Fuu-Jen); TSAI (Chang-Hai); LAI (Chien-Chen); LEI WAN; HO (Tin-Yun); HSIEH (Chang-Chi); CHAO (Pei-Dawn Lee)
AF :	Department of Medical Laboratory Science and Biotechnology, China Medical University, No. 91, Hsueh-Shih Road/Taichung 404/Taïwan (1 aut.); Clinical Virology Laboratory, Department of Laboratory Medicine, China Medical University Hospital/Taichung 404/Taïwan (1 aut.); Department of Medical Genetics and Medical Research, China Medical University Hospital/Taichung 404/Taïwan (2 aut., 3 aut., 4 aut., 5 aut.); Institute of Chinese Medical Science, China Medical University/Taichung 404/Taïwan (6 aut.); Institute of Integration Chinese and Western Medicine, China Medical University/Taichung 404/Taïwan (7 aut.); Department of Pharmacy, China Medical University/Taichung 404/Taïwan (8 aut.)
DT :	Publication en série; Niveau analytique
SO :	Antiviral research; ISSN 0166-3542; Coden ARSRDR; Pays-Bas; Da. 2005; Vol. 68; No. 1; Pp. 36-42; Bibl. 31 ref.
LA :	Anglais
EA :	The 3C-like protease (3CL^pro) of SARS-coronavirus mediates the proteolytic processing of replicase polypeptides 1a and 1ab into functional proteins, becoming an important target for the drug development. In this study, Isatis indigotica root extract, five major compounds of I. indigotica root, and seven plant-derived phenolic compounds were tested for anti-SARS-CoV 3CL^pro effects using cell-free and cell-based cleavage assays. Cleavage assays with the 3CL^pro demonstrated that IC₅₀ values were in micromolar ranges for I. indigotica root extract, indigo, sinigrin, aloe emodin and hesperetin. Sinigrin (IC₅₀: 217 μM) was more efficient in blocking the cleavage processing of the 3CL^pro than indigo (IC₅₀: 752 μM) and beta-sitosterol (IC₅₀: 1210 μM) in the cell-based assay. Only two phenolic compounds aloe emodin and hesperetin dose-dependently inhibited cleavage activity of the 3CL^pro, in which the IC₅₀ was 366 μM for aloe emodin and 8.3 μM for hesperetin in the cell-based assay.
CC :	002B02S05; 002B02A04
FD :	Virus syndrome respiratoire aigu sévère; Peptidases; Racine; Antiviral; Origine végétale; Cruciferae; Phénols; Isatis indigotica
FG :	Coronavirus; Coronaviridae; Nidovirales; Virus; Hydrolases; Enzyme; Dicotyledones; Angiospermae; Spermatophyta
ED :	Severe acute respiratory syndrome virus; Peptidases; Root; Antiviral; Plant origin; Cruciferae; Phenols
EG :	Coronavirus; Coronaviridae; Nidovirales; Virus; Hydrolases; Enzyme; Dicotyledones; Angiospermae; Spermatophyta
SD :	Severe acute respiratory syndrome virus; Peptidases; Raíz; Antiviral; Origen vegetal; Cruciferae; Fenoles
LO :	INIST-18839.354000132724070050
ID :	05-0472143

Links to Exploration step

Pascal:05-0472143

Le document en format XML

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<author><name sortKey="Chao, Pei Dawn Lee" sort="Chao, Pei Dawn Lee" uniqKey="Chao P" first="Pei-Dawn Lee" last="Chao">Pei-Dawn Lee Chao</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Anti-SARS coronavirus 3C-like protease effects of Isatis indigotica root and plant-derived phenolic compounds</title>
<author><name sortKey="Lin, Cheng Wen" sort="Lin, Cheng Wen" uniqKey="Lin C" first="Cheng-Wen" last="Lin">Cheng-Wen Lin</name>
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<author><name sortKey="Tsai, Fuu Jen" sort="Tsai, Fuu Jen" uniqKey="Tsai F" first="Fuu-Jen" last="Tsai">Fuu-Jen Tsai</name>
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<author><name sortKey="Tsai, Chang Hai" sort="Tsai, Chang Hai" uniqKey="Tsai C" first="Chang-Hai" last="Tsai">Chang-Hai Tsai</name>
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<author><name sortKey="Lai, Chien Chen" sort="Lai, Chien Chen" uniqKey="Lai C" first="Chien-Chen" last="Lai">Chien-Chen Lai</name>
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<author><name sortKey="Lei Wan" sort="Lei Wan" uniqKey="Lei Wan" last="Lei Wan">LEI WAN</name>
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<author><name sortKey="Chao, Pei Dawn Lee" sort="Chao, Pei Dawn Lee" uniqKey="Chao P" first="Pei-Dawn Lee" last="Chao">Pei-Dawn Lee Chao</name>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antiviral</term>
<term>Cruciferae</term>
<term>Peptidases</term>
<term>Phenols</term>
<term>Plant origin</term>
<term>Root</term>
<term>Severe acute respiratory syndrome virus</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Virus syndrome respiratoire aigu sévère</term>
<term>Peptidases</term>
<term>Racine</term>
<term>Antiviral</term>
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<term>Cruciferae</term>
<term>Phénols</term>
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<front><div type="abstract" xml:lang="en">The 3C-like protease (3CL<sup>pro</sup>
) of SARS-coronavirus mediates the proteolytic processing of replicase polypeptides 1a and 1ab into functional proteins, becoming an important target for the drug development. In this study, Isatis indigotica root extract, five major compounds of I. indigotica root, and seven plant-derived phenolic compounds were tested for anti-SARS-CoV 3CL<sup>pro</sup>
 effects using cell-free and cell-based cleavage assays. Cleavage assays with the 3CL<sup>pro</sup>
 demonstrated that IC<sub>50</sub>
 values were in micromolar ranges for I. indigotica root extract, indigo, sinigrin, aloe emodin and hesperetin. Sinigrin (IC<sub>50</sub>
: 217 μM) was more efficient in blocking the cleavage processing of the 3CL<sup>pro</sup>
 than indigo (IC<sub>50</sub>
: 752 μM) and beta-sitosterol (IC<sub>50</sub>
: 1210 μM) in the cell-based assay. Only two phenolic compounds aloe emodin and hesperetin dose-dependently inhibited cleavage activity of the 3CL<sup>pro</sup>
, in which the IC<sub>50</sub>
 was 366 μM for aloe emodin and 8.3 μM for hesperetin in the cell-based assay.</div>
</front>
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<fA05><s2>68</s2>
</fA05>
<fA06><s2>1</s2>
</fA06>
<fA08 i1="01" i2="1" l="ENG"><s1>Anti-SARS coronavirus 3C-like protease effects of Isatis indigotica root and plant-derived phenolic compounds</s1>
</fA08>
<fA11 i1="01" i2="1"><s1>LIN (Cheng-Wen)</s1>
</fA11>
<fA11 i1="02" i2="1"><s1>TSAI (Fuu-Jen)</s1>
</fA11>
<fA11 i1="03" i2="1"><s1>TSAI (Chang-Hai)</s1>
</fA11>
<fA11 i1="04" i2="1"><s1>LAI (Chien-Chen)</s1>
</fA11>
<fA11 i1="05" i2="1"><s1>LEI WAN</s1>
</fA11>
<fA11 i1="06" i2="1"><s1>HO (Tin-Yun)</s1>
</fA11>
<fA11 i1="07" i2="1"><s1>HSIEH (Chang-Chi)</s1>
</fA11>
<fA11 i1="08" i2="1"><s1>CHAO (Pei-Dawn Lee)</s1>
</fA11>
<fA14 i1="01"><s1>Department of Medical Laboratory Science and Biotechnology, China Medical University, No. 91, Hsueh-Shih Road</s1>
<s2>Taichung 404</s2>
<s3>TWN</s3>
<sZ>1 aut.</sZ>
</fA14>
<fA14 i1="02"><s1>Clinical Virology Laboratory, Department of Laboratory Medicine, China Medical University Hospital</s1>
<s2>Taichung 404</s2>
<s3>TWN</s3>
<sZ>1 aut.</sZ>
</fA14>
<fA14 i1="03"><s1>Department of Medical Genetics and Medical Research, China Medical University Hospital</s1>
<s2>Taichung 404</s2>
<s3>TWN</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
</fA14>
<fA14 i1="04"><s1>Institute of Chinese Medical Science, China Medical University</s1>
<s2>Taichung 404</s2>
<s3>TWN</s3>
<sZ>6 aut.</sZ>
</fA14>
<fA14 i1="05"><s1>Institute of Integration Chinese and Western Medicine, China Medical University</s1>
<s2>Taichung 404</s2>
<s3>TWN</s3>
<sZ>7 aut.</sZ>
</fA14>
<fA14 i1="06"><s1>Department of Pharmacy, China Medical University</s1>
<s2>Taichung 404</s2>
<s3>TWN</s3>
<sZ>8 aut.</sZ>
</fA14>
<fA20><s1>36-42</s1>
</fA20>
<fA21><s1>2005</s1>
</fA21>
<fA23 i1="01"><s0>ENG</s0>
</fA23>
<fA43 i1="01"><s1>INIST</s1>
<s2>18839</s2>
<s5>354000132724070050</s5>
</fA43>
<fA44><s0>0000</s0>
<s1>© 2005 INIST-CNRS. All rights reserved.</s1>
</fA44>
<fA45><s0>31 ref.</s0>
</fA45>
<fA47 i1="01" i2="1"><s0>05-0472143</s0>
</fA47>
<fA60><s1>P</s1>
</fA60>
<fA61><s0>A</s0>
</fA61>
<fA64 i1="01" i2="1"><s0>Antiviral research</s0>
</fA64>
<fA66 i1="01"><s0>NLD</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>The 3C-like protease (3CL<sup>pro</sup>
) of SARS-coronavirus mediates the proteolytic processing of replicase polypeptides 1a and 1ab into functional proteins, becoming an important target for the drug development. In this study, Isatis indigotica root extract, five major compounds of I. indigotica root, and seven plant-derived phenolic compounds were tested for anti-SARS-CoV 3CL<sup>pro</sup>
 effects using cell-free and cell-based cleavage assays. Cleavage assays with the 3CL<sup>pro</sup>
 demonstrated that IC<sub>50</sub>
 values were in micromolar ranges for I. indigotica root extract, indigo, sinigrin, aloe emodin and hesperetin. Sinigrin (IC<sub>50</sub>
: 217 μM) was more efficient in blocking the cleavage processing of the 3CL<sup>pro</sup>
 than indigo (IC<sub>50</sub>
: 752 μM) and beta-sitosterol (IC<sub>50</sub>
: 1210 μM) in the cell-based assay. Only two phenolic compounds aloe emodin and hesperetin dose-dependently inhibited cleavage activity of the 3CL<sup>pro</sup>
, in which the IC<sub>50</sub>
 was 366 μM for aloe emodin and 8.3 μM for hesperetin in the cell-based assay.</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>002B02S05</s0>
</fC02>
<fC02 i1="02" i2="X"><s0>002B02A04</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Virus syndrome respiratoire aigu sévère</s0>
<s2>NW</s2>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>Severe acute respiratory syndrome virus</s0>
<s2>NW</s2>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Severe acute respiratory syndrome virus</s0>
<s2>NW</s2>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Peptidases</s0>
<s2>FE</s2>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Peptidases</s0>
<s2>FE</s2>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Peptidases</s0>
<s2>FE</s2>
<s5>02</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Racine</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Root</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Raíz</s0>
<s5>03</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Antiviral</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Antiviral</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Antiviral</s0>
<s5>04</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Origine végétale</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Plant origin</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Origen vegetal</s0>
<s5>05</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Cruciferae</s0>
<s2>NS</s2>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Cruciferae</s0>
<s2>NS</s2>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Cruciferae</s0>
<s2>NS</s2>
<s5>06</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE"><s0>Phénols</s0>
<s2>FX</s2>
<s5>07</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG"><s0>Phenols</s0>
<s2>FX</s2>
<s5>07</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA"><s0>Fenoles</s0>
<s2>FX</s2>
<s5>07</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE"><s0>Isatis indigotica</s0>
<s4>INC</s4>
<s5>86</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Coronavirus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Coronavirus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Coronavirus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Hydrolases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Hydrolases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Hydrolases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Enzyme</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="06" i2="X" l="ENG"><s0>Enzyme</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="06" i2="X" l="SPA"><s0>Enzima</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="07" i2="X" l="FRE"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="07" i2="X" l="ENG"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="07" i2="X" l="SPA"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="08" i2="X" l="FRE"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="08" i2="X" l="ENG"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="08" i2="X" l="SPA"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="09" i2="X" l="FRE"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="09" i2="X" l="ENG"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="09" i2="X" l="SPA"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fN21><s1>332</s1>
</fN21>
</pA>
</standard>
<server><NO>PASCAL 05-0472143 INIST</NO>
<ET>Anti-SARS coronavirus 3C-like protease effects of Isatis indigotica root and plant-derived phenolic compounds</ET>
<AU>LIN (Cheng-Wen); TSAI (Fuu-Jen); TSAI (Chang-Hai); LAI (Chien-Chen); LEI WAN; HO (Tin-Yun); HSIEH (Chang-Chi); CHAO (Pei-Dawn Lee)</AU>
<AF>Department of Medical Laboratory Science and Biotechnology, China Medical University, No. 91, Hsueh-Shih Road/Taichung 404/Taïwan (1 aut.); Clinical Virology Laboratory, Department of Laboratory Medicine, China Medical University Hospital/Taichung 404/Taïwan (1 aut.); Department of Medical Genetics and Medical Research, China Medical University Hospital/Taichung 404/Taïwan (2 aut., 3 aut., 4 aut., 5 aut.); Institute of Chinese Medical Science, China Medical University/Taichung 404/Taïwan (6 aut.); Institute of Integration Chinese and Western Medicine, China Medical University/Taichung 404/Taïwan (7 aut.); Department of Pharmacy, China Medical University/Taichung 404/Taïwan (8 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Antiviral research; ISSN 0166-3542; Coden ARSRDR; Pays-Bas; Da. 2005; Vol. 68; No. 1; Pp. 36-42; Bibl. 31 ref.</SO>
<LA>Anglais</LA>
<EA>The 3C-like protease (3CL<sup>pro</sup>
) of SARS-coronavirus mediates the proteolytic processing of replicase polypeptides 1a and 1ab into functional proteins, becoming an important target for the drug development. In this study, Isatis indigotica root extract, five major compounds of I. indigotica root, and seven plant-derived phenolic compounds were tested for anti-SARS-CoV 3CL<sup>pro</sup>
 effects using cell-free and cell-based cleavage assays. Cleavage assays with the 3CL<sup>pro</sup>
 demonstrated that IC<sub>50</sub>
 values were in micromolar ranges for I. indigotica root extract, indigo, sinigrin, aloe emodin and hesperetin. Sinigrin (IC<sub>50</sub>
: 217 μM) was more efficient in blocking the cleavage processing of the 3CL<sup>pro</sup>
 than indigo (IC<sub>50</sub>
: 752 μM) and beta-sitosterol (IC<sub>50</sub>
: 1210 μM) in the cell-based assay. Only two phenolic compounds aloe emodin and hesperetin dose-dependently inhibited cleavage activity of the 3CL<sup>pro</sup>
, in which the IC<sub>50</sub>
 was 366 μM for aloe emodin and 8.3 μM for hesperetin in the cell-based assay.</EA>
<CC>002B02S05; 002B02A04</CC>
<FD>Virus syndrome respiratoire aigu sévère; Peptidases; Racine; Antiviral; Origine végétale; Cruciferae; Phénols; Isatis indigotica</FD>
<FG>Coronavirus; Coronaviridae; Nidovirales; Virus; Hydrolases; Enzyme; Dicotyledones; Angiospermae; Spermatophyta</FG>
<ED>Severe acute respiratory syndrome virus; Peptidases; Root; Antiviral; Plant origin; Cruciferae; Phenols</ED>
<EG>Coronavirus; Coronaviridae; Nidovirales; Virus; Hydrolases; Enzyme; Dicotyledones; Angiospermae; Spermatophyta</EG>
<SD>Severe acute respiratory syndrome virus; Peptidases; Raíz; Antiviral; Origen vegetal; Cruciferae; Fenoles</SD>
<LO>INIST-18839.354000132724070050</LO>
<ID>05-0472143</ID>
</server>
</inist>
</record>

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Anti-SARS coronavirus 3C-like protease effects of Isatis indigotica root and plant-derived phenolic compounds

Anti-SARS coronavirus 3C-like protease effects of Isatis indigotica root and plant-derived phenolic compounds

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