Anti-SARS coronavirus 3C-like protease effects of Isatis indigotica root and plant-derived phenolic compounds
Identifieur interne : 000587 ( PascalFrancis/Corpus ); précédent : 000586; suivant : 000588Anti-SARS coronavirus 3C-like protease effects of Isatis indigotica root and plant-derived phenolic compounds
Auteurs : Cheng-Wen Lin ; Fuu-Jen Tsai ; Chang-Hai Tsai ; Chien-Chen Lai ; LEI WAN ; Tin-Yun Ho ; Chang-Chi Hsieh ; Pei-Dawn Lee ChaoSource :
- Antiviral research [ 0166-3542 ] ; 2005.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
The 3C-like protease (3CLpro) of SARS-coronavirus mediates the proteolytic processing of replicase polypeptides 1a and 1ab into functional proteins, becoming an important target for the drug development. In this study, Isatis indigotica root extract, five major compounds of I. indigotica root, and seven plant-derived phenolic compounds were tested for anti-SARS-CoV 3CLpro effects using cell-free and cell-based cleavage assays. Cleavage assays with the 3CLpro demonstrated that IC50 values were in micromolar ranges for I. indigotica root extract, indigo, sinigrin, aloe emodin and hesperetin. Sinigrin (IC50: 217 μM) was more efficient in blocking the cleavage processing of the 3CLpro than indigo (IC50: 752 μM) and beta-sitosterol (IC50: 1210 μM) in the cell-based assay. Only two phenolic compounds aloe emodin and hesperetin dose-dependently inhibited cleavage activity of the 3CLpro, in which the IC50 was 366 μM for aloe emodin and 8.3 μM for hesperetin in the cell-based assay.
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Pour connaître la documentation sur le format Inist Standard.
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Format Inist (serveur)
NO : | PASCAL 05-0472143 INIST |
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ET : | Anti-SARS coronavirus 3C-like protease effects of Isatis indigotica root and plant-derived phenolic compounds |
AU : | LIN (Cheng-Wen); TSAI (Fuu-Jen); TSAI (Chang-Hai); LAI (Chien-Chen); LEI WAN; HO (Tin-Yun); HSIEH (Chang-Chi); CHAO (Pei-Dawn Lee) |
AF : | Department of Medical Laboratory Science and Biotechnology, China Medical University, No. 91, Hsueh-Shih Road/Taichung 404/Taïwan (1 aut.); Clinical Virology Laboratory, Department of Laboratory Medicine, China Medical University Hospital/Taichung 404/Taïwan (1 aut.); Department of Medical Genetics and Medical Research, China Medical University Hospital/Taichung 404/Taïwan (2 aut., 3 aut., 4 aut., 5 aut.); Institute of Chinese Medical Science, China Medical University/Taichung 404/Taïwan (6 aut.); Institute of Integration Chinese and Western Medicine, China Medical University/Taichung 404/Taïwan (7 aut.); Department of Pharmacy, China Medical University/Taichung 404/Taïwan (8 aut.) |
DT : | Publication en série; Niveau analytique |
SO : | Antiviral research; ISSN 0166-3542; Coden ARSRDR; Pays-Bas; Da. 2005; Vol. 68; No. 1; Pp. 36-42; Bibl. 31 ref. |
LA : | Anglais |
EA : | The 3C-like protease (3CLpro) of SARS-coronavirus mediates the proteolytic processing of replicase polypeptides 1a and 1ab into functional proteins, becoming an important target for the drug development. In this study, Isatis indigotica root extract, five major compounds of I. indigotica root, and seven plant-derived phenolic compounds were tested for anti-SARS-CoV 3CLpro effects using cell-free and cell-based cleavage assays. Cleavage assays with the 3CLpro demonstrated that IC50 values were in micromolar ranges for I. indigotica root extract, indigo, sinigrin, aloe emodin and hesperetin. Sinigrin (IC50: 217 μM) was more efficient in blocking the cleavage processing of the 3CLpro than indigo (IC50: 752 μM) and beta-sitosterol (IC50: 1210 μM) in the cell-based assay. Only two phenolic compounds aloe emodin and hesperetin dose-dependently inhibited cleavage activity of the 3CLpro, in which the IC50 was 366 μM for aloe emodin and 8.3 μM for hesperetin in the cell-based assay. |
CC : | 002B02S05; 002B02A04 |
FD : | Virus syndrome respiratoire aigu sévère; Peptidases; Racine; Antiviral; Origine végétale; Cruciferae; Phénols; Isatis indigotica |
FG : | Coronavirus; Coronaviridae; Nidovirales; Virus; Hydrolases; Enzyme; Dicotyledones; Angiospermae; Spermatophyta |
ED : | Severe acute respiratory syndrome virus; Peptidases; Root; Antiviral; Plant origin; Cruciferae; Phenols |
EG : | Coronavirus; Coronaviridae; Nidovirales; Virus; Hydrolases; Enzyme; Dicotyledones; Angiospermae; Spermatophyta |
SD : | Severe acute respiratory syndrome virus; Peptidases; Raíz; Antiviral; Origen vegetal; Cruciferae; Fenoles |
LO : | INIST-18839.354000132724070050 |
ID : | 05-0472143 |
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Pascal:05-0472143Le document en format XML
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<front><div type="abstract" xml:lang="en">The 3C-like protease (3CL<sup>pro</sup>
) of SARS-coronavirus mediates the proteolytic processing of replicase polypeptides 1a and 1ab into functional proteins, becoming an important target for the drug development. In this study, Isatis indigotica root extract, five major compounds of I. indigotica root, and seven plant-derived phenolic compounds were tested for anti-SARS-CoV 3CL<sup>pro</sup>
effects using cell-free and cell-based cleavage assays. Cleavage assays with the 3CL<sup>pro</sup>
demonstrated that IC<sub>50</sub>
values were in micromolar ranges for I. indigotica root extract, indigo, sinigrin, aloe emodin and hesperetin. Sinigrin (IC<sub>50</sub>
: 217 μM) was more efficient in blocking the cleavage processing of the 3CL<sup>pro</sup>
than indigo (IC<sub>50</sub>
: 752 μM) and beta-sitosterol (IC<sub>50</sub>
: 1210 μM) in the cell-based assay. Only two phenolic compounds aloe emodin and hesperetin dose-dependently inhibited cleavage activity of the 3CL<sup>pro</sup>
, in which the IC<sub>50</sub>
was 366 μM for aloe emodin and 8.3 μM for hesperetin in the cell-based assay.</div>
</front>
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<fA11 i1="01" i2="1"><s1>LIN (Cheng-Wen)</s1>
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<fA11 i1="02" i2="1"><s1>TSAI (Fuu-Jen)</s1>
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<fA14 i1="03"><s1>Department of Medical Genetics and Medical Research, China Medical University Hospital</s1>
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<fA14 i1="05"><s1>Institute of Integration Chinese and Western Medicine, China Medical University</s1>
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<sZ>7 aut.</sZ>
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<fA14 i1="06"><s1>Department of Pharmacy, China Medical University</s1>
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effects using cell-free and cell-based cleavage assays. Cleavage assays with the 3CL<sup>pro</sup>
demonstrated that IC<sub>50</sub>
values were in micromolar ranges for I. indigotica root extract, indigo, sinigrin, aloe emodin and hesperetin. Sinigrin (IC<sub>50</sub>
: 217 μM) was more efficient in blocking the cleavage processing of the 3CL<sup>pro</sup>
than indigo (IC<sub>50</sub>
: 752 μM) and beta-sitosterol (IC<sub>50</sub>
: 1210 μM) in the cell-based assay. Only two phenolic compounds aloe emodin and hesperetin dose-dependently inhibited cleavage activity of the 3CL<sup>pro</sup>
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<s5>07</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG"><s0>Phenols</s0>
<s2>FX</s2>
<s5>07</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA"><s0>Fenoles</s0>
<s2>FX</s2>
<s5>07</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE"><s0>Isatis indigotica</s0>
<s4>INC</s4>
<s5>86</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Coronavirus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Coronavirus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Coronavirus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Hydrolases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Hydrolases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Hydrolases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Enzyme</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="06" i2="X" l="ENG"><s0>Enzyme</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="06" i2="X" l="SPA"><s0>Enzima</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="07" i2="X" l="FRE"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="07" i2="X" l="ENG"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="07" i2="X" l="SPA"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="08" i2="X" l="FRE"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="08" i2="X" l="ENG"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="08" i2="X" l="SPA"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="09" i2="X" l="FRE"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="09" i2="X" l="ENG"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="09" i2="X" l="SPA"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fN21><s1>332</s1>
</fN21>
</pA>
</standard>
<server><NO>PASCAL 05-0472143 INIST</NO>
<ET>Anti-SARS coronavirus 3C-like protease effects of Isatis indigotica root and plant-derived phenolic compounds</ET>
<AU>LIN (Cheng-Wen); TSAI (Fuu-Jen); TSAI (Chang-Hai); LAI (Chien-Chen); LEI WAN; HO (Tin-Yun); HSIEH (Chang-Chi); CHAO (Pei-Dawn Lee)</AU>
<AF>Department of Medical Laboratory Science and Biotechnology, China Medical University, No. 91, Hsueh-Shih Road/Taichung 404/Taïwan (1 aut.); Clinical Virology Laboratory, Department of Laboratory Medicine, China Medical University Hospital/Taichung 404/Taïwan (1 aut.); Department of Medical Genetics and Medical Research, China Medical University Hospital/Taichung 404/Taïwan (2 aut., 3 aut., 4 aut., 5 aut.); Institute of Chinese Medical Science, China Medical University/Taichung 404/Taïwan (6 aut.); Institute of Integration Chinese and Western Medicine, China Medical University/Taichung 404/Taïwan (7 aut.); Department of Pharmacy, China Medical University/Taichung 404/Taïwan (8 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Antiviral research; ISSN 0166-3542; Coden ARSRDR; Pays-Bas; Da. 2005; Vol. 68; No. 1; Pp. 36-42; Bibl. 31 ref.</SO>
<LA>Anglais</LA>
<EA>The 3C-like protease (3CL<sup>pro</sup>
) of SARS-coronavirus mediates the proteolytic processing of replicase polypeptides 1a and 1ab into functional proteins, becoming an important target for the drug development. In this study, Isatis indigotica root extract, five major compounds of I. indigotica root, and seven plant-derived phenolic compounds were tested for anti-SARS-CoV 3CL<sup>pro</sup>
effects using cell-free and cell-based cleavage assays. Cleavage assays with the 3CL<sup>pro</sup>
demonstrated that IC<sub>50</sub>
values were in micromolar ranges for I. indigotica root extract, indigo, sinigrin, aloe emodin and hesperetin. Sinigrin (IC<sub>50</sub>
: 217 μM) was more efficient in blocking the cleavage processing of the 3CL<sup>pro</sup>
than indigo (IC<sub>50</sub>
: 752 μM) and beta-sitosterol (IC<sub>50</sub>
: 1210 μM) in the cell-based assay. Only two phenolic compounds aloe emodin and hesperetin dose-dependently inhibited cleavage activity of the 3CL<sup>pro</sup>
, in which the IC<sub>50</sub>
was 366 μM for aloe emodin and 8.3 μM for hesperetin in the cell-based assay.</EA>
<CC>002B02S05; 002B02A04</CC>
<FD>Virus syndrome respiratoire aigu sévère; Peptidases; Racine; Antiviral; Origine végétale; Cruciferae; Phénols; Isatis indigotica</FD>
<FG>Coronavirus; Coronaviridae; Nidovirales; Virus; Hydrolases; Enzyme; Dicotyledones; Angiospermae; Spermatophyta</FG>
<ED>Severe acute respiratory syndrome virus; Peptidases; Root; Antiviral; Plant origin; Cruciferae; Phenols</ED>
<EG>Coronavirus; Coronaviridae; Nidovirales; Virus; Hydrolases; Enzyme; Dicotyledones; Angiospermae; Spermatophyta</EG>
<SD>Severe acute respiratory syndrome virus; Peptidases; Raíz; Antiviral; Origen vegetal; Cruciferae; Fenoles</SD>
<LO>INIST-18839.354000132724070050</LO>
<ID>05-0472143</ID>
</server>
</inist>
</record>
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