Recombinant protein-based elisa and immuno-cytochemical assay for the diagnosis of sars
Identifieur interne : 000582 ( PascalFrancis/Corpus ); précédent : 000581; suivant : 000583Recombinant protein-based elisa and immuno-cytochemical assay for the diagnosis of sars
Auteurs : Alessandra Carattoli ; Paola Di Bonito ; Felicia Grasso ; Colomba Giorgi ; Francesco Blasi ; Matthias Niedrig ; Antonio CassoneSource :
- Journal of medical virology [ 0146-6615 ] ; 2005.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
A new Coronavirus (SARS-CoV) is the aetiological agent of the severe acute respiratory syndrome (SARS). Because of the critical role played by serological assays for SARS diagnosis, an in-house ELISA based on SARS-CoV recombinant antigens was developed. The SARS-CoV nucleocapsid protein (N), three N fragments (N1, N2, and N3) and the intraviral domain of the membrane protein (M2) were cloned and expressed in Escherichia coli as histidine-tagged proteins. Six reference sera from SARS patients were used to detect virus-specific IgG in an ELISA using each recombinant protein as coating antigen. High-titre positive reactions were detected in all SARS positive sera. The specificity of the assay appears to be high as no positive reaction was detected in the sera of 20 healthy subjects and 73 patients with non-SARS, low-tract respiratory infections. Specific hyper-immune sera to SARS-CoV and the recombinant proteins, N, N1, N2, N3, and M2 were also generated in mice and rabbits. The specificity of these sera was confirmed by an immunocytochemical assay on biochips of SARS-CoV infected and uninfected cells.
Notice en format standard (ISO 2709)
Pour connaître la documentation sur le format Inist Standard.
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Format Inist (serveur)
NO : | PASCAL 05-0484475 INIST |
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ET : | Recombinant protein-based elisa and immuno-cytochemical assay for the diagnosis of sars |
AU : | CARATTOLI (Alessandra); DI BONITO (Paola); GRASSO (Felicia); GIORGI (Colomba); BLASI (Francesco); NIEDRIG (Matthias); CASSONE (Antonio) |
AF : | Department of Infectious, Parasitic and Immuno-Mediated Diseases, Istituto Superiore di Sanità/Rome/Italie (1 aut., 2 aut., 3 aut., 4 aut., 7 aut.); Ospedale Maggiore Policlinico, University of Milan/Milan/Italie (5 aut.); Robert Koch Institut/Berlin/Allemagne (6 aut.) |
DT : | Publication en série; Niveau analytique |
SO : | Journal of medical virology; ISSN 0146-6615; Coden JMVIDB; Etats-Unis; Da. 2005; Vol. 76; No. 2; Pp. 137-142; Bibl. 17 ref. |
LA : | Anglais |
EA : | A new Coronavirus (SARS-CoV) is the aetiological agent of the severe acute respiratory syndrome (SARS). Because of the critical role played by serological assays for SARS diagnosis, an in-house ELISA based on SARS-CoV recombinant antigens was developed. The SARS-CoV nucleocapsid protein (N), three N fragments (N1, N2, and N3) and the intraviral domain of the membrane protein (M2) were cloned and expressed in Escherichia coli as histidine-tagged proteins. Six reference sera from SARS patients were used to detect virus-specific IgG in an ELISA using each recombinant protein as coating antigen. High-titre positive reactions were detected in all SARS positive sera. The specificity of the assay appears to be high as no positive reaction was detected in the sera of 20 healthy subjects and 73 patients with non-SARS, low-tract respiratory infections. Specific hyper-immune sera to SARS-CoV and the recombinant proteins, N, N1, N2, N3, and M2 were also generated in mice and rabbits. The specificity of these sera was confirmed by an immunocytochemical assay on biochips of SARS-CoV infected and uninfected cells. |
CC : | 002A05C10; 002B05C02J |
FD : | Coronavirus; Protéine recombinante; Technique ELISA; Diagnostic; Anticorps; Sérologie; Syndrome respiratoire aigu sévère |
FG : | Coronaviridae; Nidovirales; Virus; Appareil respiratoire pathologie; Virose; Infection; Poumon pathologie |
ED : | Coronavirus; Recombinant protein; ELISA assay; Diagnosis; Antibody; Serology; Severe acute respiratory syndrome |
EG : | Coronaviridae; Nidovirales; Virus; Respiratory disease; Viral disease; Infection; Lung disease |
SD : | Coronavirus; Proteína recombinante; Técnica ELISA; Diagnóstico; Anticuerpo; Serología; Síndrome respiratorio agudo severo |
LO : | INIST-17422.354000129811490010 |
ID : | 05-0484475 |
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Pascal:05-0484475Le document en format XML
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<front><div type="abstract" xml:lang="en">A new Coronavirus (SARS-CoV) is the aetiological agent of the severe acute respiratory syndrome (SARS). Because of the critical role played by serological assays for SARS diagnosis, an in-house ELISA based on SARS-CoV recombinant antigens was developed. The SARS-CoV nucleocapsid protein (N), three N fragments (N1, N2, and N3) and the intraviral domain of the membrane protein (M2) were cloned and expressed in Escherichia coli as histidine-tagged proteins. Six reference sera from SARS patients were used to detect virus-specific IgG in an ELISA using each recombinant protein as coating antigen. High-titre positive reactions were detected in all SARS positive sera. The specificity of the assay appears to be high as no positive reaction was detected in the sera of 20 healthy subjects and 73 patients with non-SARS, low-tract respiratory infections. Specific hyper-immune sera to SARS-CoV and the recombinant proteins, N, N1, N2, N3, and M2 were also generated in mice and rabbits. The specificity of these sera was confirmed by an immunocytochemical assay on biochips of SARS-CoV infected and uninfected cells.</div>
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<server><NO>PASCAL 05-0484475 INIST</NO>
<ET>Recombinant protein-based elisa and immuno-cytochemical assay for the diagnosis of sars</ET>
<AU>CARATTOLI (Alessandra); DI BONITO (Paola); GRASSO (Felicia); GIORGI (Colomba); BLASI (Francesco); NIEDRIG (Matthias); CASSONE (Antonio)</AU>
<AF>Department of Infectious, Parasitic and Immuno-Mediated Diseases, Istituto Superiore di Sanità/Rome/Italie (1 aut., 2 aut., 3 aut., 4 aut., 7 aut.); Ospedale Maggiore Policlinico, University of Milan/Milan/Italie (5 aut.); Robert Koch Institut/Berlin/Allemagne (6 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Journal of medical virology; ISSN 0146-6615; Coden JMVIDB; Etats-Unis; Da. 2005; Vol. 76; No. 2; Pp. 137-142; Bibl. 17 ref.</SO>
<LA>Anglais</LA>
<EA>A new Coronavirus (SARS-CoV) is the aetiological agent of the severe acute respiratory syndrome (SARS). Because of the critical role played by serological assays for SARS diagnosis, an in-house ELISA based on SARS-CoV recombinant antigens was developed. The SARS-CoV nucleocapsid protein (N), three N fragments (N1, N2, and N3) and the intraviral domain of the membrane protein (M2) were cloned and expressed in Escherichia coli as histidine-tagged proteins. Six reference sera from SARS patients were used to detect virus-specific IgG in an ELISA using each recombinant protein as coating antigen. High-titre positive reactions were detected in all SARS positive sera. The specificity of the assay appears to be high as no positive reaction was detected in the sera of 20 healthy subjects and 73 patients with non-SARS, low-tract respiratory infections. Specific hyper-immune sera to SARS-CoV and the recombinant proteins, N, N1, N2, N3, and M2 were also generated in mice and rabbits. The specificity of these sera was confirmed by an immunocytochemical assay on biochips of SARS-CoV infected and uninfected cells.</EA>
<CC>002A05C10; 002B05C02J</CC>
<FD>Coronavirus; Protéine recombinante; Technique ELISA; Diagnostic; Anticorps; Sérologie; Syndrome respiratoire aigu sévère</FD>
<FG>Coronaviridae; Nidovirales; Virus; Appareil respiratoire pathologie; Virose; Infection; Poumon pathologie</FG>
<ED>Coronavirus; Recombinant protein; ELISA assay; Diagnosis; Antibody; Serology; Severe acute respiratory syndrome</ED>
<EG>Coronaviridae; Nidovirales; Virus; Respiratory disease; Viral disease; Infection; Lung disease</EG>
<SD>Coronavirus; Proteína recombinante; Técnica ELISA; Diagnóstico; Anticuerpo; Serología; Síndrome respiratorio agudo severo</SD>
<LO>INIST-17422.354000129811490010</LO>
<ID>05-0484475</ID>
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