SrasV1, PascalFrancis, Corpus, bibRecord, 000582

Recombinant protein-based elisa and immuno-cytochemical assay for the diagnosis of sars

Identifieur interne : 000582 ( PascalFrancis/Corpus ); précédent : 000581; suivant : 000583

Recombinant protein-based elisa and immuno-cytochemical assay for the diagnosis of sars

Auteurs : Alessandra Carattoli ; Paola Di Bonito ; Felicia Grasso ; Colomba Giorgi ; Francesco Blasi ; Matthias Niedrig ; Antonio Cassone

Source :

Journal of medical virology [ 0146-6615 ] ; 2005.

RBID : Pascal:05-0484475

Descripteurs français

Pascal (Inist)
- Coronavirus, Protéine recombinante, Technique ELISA, Diagnostic, Anticorps, Sérologie, Syndrome respiratoire aigu sévère.

English descriptors

KwdEn :
- Antibody, Coronavirus, Diagnosis, ELISA assay, Recombinant protein, Serology, Severe acute respiratory syndrome.

Abstract

A new Coronavirus (SARS-CoV) is the aetiological agent of the severe acute respiratory syndrome (SARS). Because of the critical role played by serological assays for SARS diagnosis, an in-house ELISA based on SARS-CoV recombinant antigens was developed. The SARS-CoV nucleocapsid protein (N), three N fragments (N1, N2, and N3) and the intraviral domain of the membrane protein (M2) were cloned and expressed in Escherichia coli as histidine-tagged proteins. Six reference sera from SARS patients were used to detect virus-specific IgG in an ELISA using each recombinant protein as coating antigen. High-titre positive reactions were detected in all SARS positive sera. The specificity of the assay appears to be high as no positive reaction was detected in the sera of 20 healthy subjects and 73 patients with non-SARS, low-tract respiratory infections. Specific hyper-immune sera to SARS-CoV and the recombinant proteins, N, N1, N2, N3, and M2 were also generated in mice and rabbits. The specificity of these sera was confirmed by an immunocytochemical assay on biochips of SARS-CoV infected and uninfected cells.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

A01	`01`	`1`		`@0 0146-6615`
A02	`01`			`@0 JMVIDB`
A03		`1`		`@0 J. med. virol.`
A05				`@2 76`
A06				`@2 2`
A08	`01`	`1`	`ENG`	`@1 Recombinant protein-based elisa and immuno-cytochemical assay for the diagnosis of sars`
A11	`01`	`1`		`@1 CARATTOLI (Alessandra)`
A11	`02`	`1`		`@1 DI BONITO (Paola)`
A11	`03`	`1`		`@1 GRASSO (Felicia)`
A11	`04`	`1`		`@1 GIORGI (Colomba)`
A11	`05`	`1`		`@1 BLASI (Francesco)`
A11	`06`	`1`		`@1 NIEDRIG (Matthias)`
A11	`07`	`1`		`@1 CASSONE (Antonio)`
A14	`01`			`@1 Department of Infectious, Parasitic and Immuno-Mediated Diseases, Istituto Superiore di Sanità @2 Rome @3 ITA @Z 1 aut. @Z 2 aut. @Z 3 aut. @Z 4 aut. @Z 7 aut.`
A14	`02`			`@1 Ospedale Maggiore Policlinico, University of Milan @2 Milan @3 ITA @Z 5 aut.`
A14	`03`			`@1 Robert Koch Institut @2 Berlin @3 DEU @Z 6 aut.`
A20				`@1 137-142`
A21				`@1 2005`
A23	`01`			`@0 ENG`
A43	`01`			`@1 INIST @2 17422 @5 354000129811490010`
A44				`@0 0000 @1 © 2005 INIST-CNRS. All rights reserved.`
A45				`@0 17 ref.`
A47	`01`	`1`		`@0 05-0484475`
A60				`@1 P`
A61				`@0 A`
A64	`01`	`1`		`@0 Journal of medical virology`
A66	`01`			`@0 USA`
C01	`01`		`ENG`	@0 A new Coronavirus (SARS-CoV) is the aetiological agent of the severe acute respiratory syndrome (SARS). Because of the critical role played by serological assays for SARS diagnosis, an in-house ELISA based on SARS-CoV recombinant antigens was developed. The SARS-CoV nucleocapsid protein (N), three N fragments (N1, N2, and N3) and the intraviral domain of the membrane protein (M2) were cloned and expressed in Escherichia coli as histidine-tagged proteins. Six reference sera from SARS patients were used to detect virus-specific IgG in an ELISA using each recombinant protein as coating antigen. High-titre positive reactions were detected in all SARS positive sera. The specificity of the assay appears to be high as no positive reaction was detected in the sera of 20 healthy subjects and 73 patients with non-SARS, low-tract respiratory infections. Specific hyper-immune sera to SARS-CoV and the recombinant proteins, N, N1, N2, N3, and M2 were also generated in mice and rabbits. The specificity of these sera was confirmed by an immunocytochemical assay on biochips of SARS-CoV infected and uninfected cells.
C02	`01`	`X`		`@0 002A05C10`
C02	`02`	`X`		`@0 002B05C02J`
C03	`01`	`X`	`FRE`	`@0 Coronavirus @2 NW @5 01`
C03	`01`	`X`	`ENG`	`@0 Coronavirus @2 NW @5 01`
C03	`01`	`X`	`SPA`	`@0 Coronavirus @2 NW @5 01`
C03	`02`	`X`	`FRE`	`@0 Protéine recombinante @5 05`
C03	`02`	`X`	`ENG`	`@0 Recombinant protein @5 05`
C03	`02`	`X`	`SPA`	`@0 Proteína recombinante @5 05`
C03	`03`	`X`	`FRE`	`@0 Technique ELISA @5 06`
C03	`03`	`X`	`ENG`	`@0 ELISA assay @5 06`
C03	`03`	`X`	`SPA`	`@0 Técnica ELISA @5 06`
C03	`04`	`X`	`FRE`	`@0 Diagnostic @5 07`
C03	`04`	`X`	`ENG`	`@0 Diagnosis @5 07`
C03	`04`	`X`	`SPA`	`@0 Diagnóstico @5 07`
C03	`05`	`X`	`FRE`	`@0 Anticorps @5 08`
C03	`05`	`X`	`ENG`	`@0 Antibody @5 08`
C03	`05`	`X`	`SPA`	`@0 Anticuerpo @5 08`
C03	`06`	`X`	`FRE`	`@0 Sérologie @5 09`
C03	`06`	`X`	`ENG`	`@0 Serology @5 09`
C03	`06`	`X`	`SPA`	`@0 Serología @5 09`
C03	`07`	`X`	`FRE`	`@0 Syndrome respiratoire aigu sévère @2 NM @5 14`
C03	`07`	`X`	`ENG`	`@0 Severe acute respiratory syndrome @2 NM @5 14`
C03	`07`	`X`	`SPA`	`@0 Síndrome respiratorio agudo severo @2 NM @5 14`
C07	`01`	`X`	`FRE`	`@0 Coronaviridae @2 NW`
C07	`01`	`X`	`ENG`	`@0 Coronaviridae @2 NW`
C07	`01`	`X`	`SPA`	`@0 Coronaviridae @2 NW`
C07	`02`	`X`	`FRE`	`@0 Nidovirales @2 NW`
C07	`02`	`X`	`ENG`	`@0 Nidovirales @2 NW`
C07	`02`	`X`	`SPA`	`@0 Nidovirales @2 NW`
C07	`03`	`X`	`FRE`	`@0 Virus @2 NW`
C07	`03`	`X`	`ENG`	`@0 Virus @2 NW`
C07	`03`	`X`	`SPA`	`@0 Virus @2 NW`
C07	`04`	`X`	`FRE`	`@0 Appareil respiratoire pathologie @5 13`
C07	`04`	`X`	`ENG`	`@0 Respiratory disease @5 13`
C07	`04`	`X`	`SPA`	`@0 Aparato respiratorio patología @5 13`
C07	`05`	`X`	`FRE`	`@0 Virose`
C07	`05`	`X`	`ENG`	`@0 Viral disease`
C07	`05`	`X`	`SPA`	`@0 Virosis`
C07	`06`	`X`	`FRE`	`@0 Infection`
C07	`06`	`X`	`ENG`	`@0 Infection`
C07	`06`	`X`	`SPA`	`@0 Infección`
C07	`07`	`X`	`FRE`	`@0 Poumon pathologie @5 16`
C07	`07`	`X`	`ENG`	`@0 Lung disease @5 16`
C07	`07`	`X`	`SPA`	`@0 Pulmón patología @5 16`
N21				`@1 339`

Format Inist (serveur)

NO :	PASCAL 05-0484475 INIST
ET :	Recombinant protein-based elisa and immuno-cytochemical assay for the diagnosis of sars
AU :	CARATTOLI (Alessandra); DI BONITO (Paola); GRASSO (Felicia); GIORGI (Colomba); BLASI (Francesco); NIEDRIG (Matthias); CASSONE (Antonio)
AF :	Department of Infectious, Parasitic and Immuno-Mediated Diseases, Istituto Superiore di Sanità/Rome/Italie (1 aut., 2 aut., 3 aut., 4 aut., 7 aut.); Ospedale Maggiore Policlinico, University of Milan/Milan/Italie (5 aut.); Robert Koch Institut/Berlin/Allemagne (6 aut.)
DT :	Publication en série; Niveau analytique
SO :	Journal of medical virology; ISSN 0146-6615; Coden JMVIDB; Etats-Unis; Da. 2005; Vol. 76; No. 2; Pp. 137-142; Bibl. 17 ref.
LA :	Anglais
EA :	A new Coronavirus (SARS-CoV) is the aetiological agent of the severe acute respiratory syndrome (SARS). Because of the critical role played by serological assays for SARS diagnosis, an in-house ELISA based on SARS-CoV recombinant antigens was developed. The SARS-CoV nucleocapsid protein (N), three N fragments (N1, N2, and N3) and the intraviral domain of the membrane protein (M2) were cloned and expressed in Escherichia coli as histidine-tagged proteins. Six reference sera from SARS patients were used to detect virus-specific IgG in an ELISA using each recombinant protein as coating antigen. High-titre positive reactions were detected in all SARS positive sera. The specificity of the assay appears to be high as no positive reaction was detected in the sera of 20 healthy subjects and 73 patients with non-SARS, low-tract respiratory infections. Specific hyper-immune sera to SARS-CoV and the recombinant proteins, N, N1, N2, N3, and M2 were also generated in mice and rabbits. The specificity of these sera was confirmed by an immunocytochemical assay on biochips of SARS-CoV infected and uninfected cells.
CC :	002A05C10; 002B05C02J
FD :	Coronavirus; Protéine recombinante; Technique ELISA; Diagnostic; Anticorps; Sérologie; Syndrome respiratoire aigu sévère
FG :	Coronaviridae; Nidovirales; Virus; Appareil respiratoire pathologie; Virose; Infection; Poumon pathologie
ED :	Coronavirus; Recombinant protein; ELISA assay; Diagnosis; Antibody; Serology; Severe acute respiratory syndrome
EG :	Coronaviridae; Nidovirales; Virus; Respiratory disease; Viral disease; Infection; Lung disease
SD :	Coronavirus; Proteína recombinante; Técnica ELISA; Diagnóstico; Anticuerpo; Serología; Síndrome respiratorio agudo severo
LO :	INIST-17422.354000129811490010
ID :	05-0484475

Links to Exploration step

Pascal:05-0484475

Le document en format XML

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<front><div type="abstract" xml:lang="en">A new Coronavirus (SARS-CoV) is the aetiological agent of the severe acute respiratory syndrome (SARS). Because of the critical role played by serological assays for SARS diagnosis, an in-house ELISA based on SARS-CoV recombinant antigens was developed. The SARS-CoV nucleocapsid protein (N), three N fragments (N1, N2, and N3) and the intraviral domain of the membrane protein (M2) were cloned and expressed in Escherichia coli as histidine-tagged proteins. Six reference sera from SARS patients were used to detect virus-specific IgG in an ELISA using each recombinant protein as coating antigen. High-titre positive reactions were detected in all SARS positive sera. The specificity of the assay appears to be high as no positive reaction was detected in the sera of 20 healthy subjects and 73 patients with non-SARS, low-tract respiratory infections. Specific hyper-immune sera to SARS-CoV and the recombinant proteins, N, N1, N2, N3, and M2 were also generated in mice and rabbits. The specificity of these sera was confirmed by an immunocytochemical assay on biochips of SARS-CoV infected and uninfected cells.</div>
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<fC01 i1="01" l="ENG"><s0>A new Coronavirus (SARS-CoV) is the aetiological agent of the severe acute respiratory syndrome (SARS). Because of the critical role played by serological assays for SARS diagnosis, an in-house ELISA based on SARS-CoV recombinant antigens was developed. The SARS-CoV nucleocapsid protein (N), three N fragments (N1, N2, and N3) and the intraviral domain of the membrane protein (M2) were cloned and expressed in Escherichia coli as histidine-tagged proteins. Six reference sera from SARS patients were used to detect virus-specific IgG in an ELISA using each recombinant protein as coating antigen. High-titre positive reactions were detected in all SARS positive sera. The specificity of the assay appears to be high as no positive reaction was detected in the sera of 20 healthy subjects and 73 patients with non-SARS, low-tract respiratory infections. Specific hyper-immune sera to SARS-CoV and the recombinant proteins, N, N1, N2, N3, and M2 were also generated in mice and rabbits. The specificity of these sera was confirmed by an immunocytochemical assay on biochips of SARS-CoV infected and uninfected cells.</s0>
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<fC02 i1="02" i2="X"><s0>002B05C02J</s0>
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<s5>07</s5>
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<s5>08</s5>
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<s5>08</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Anticuerpo</s0>
<s5>08</s5>
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<s5>09</s5>
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<s5>09</s5>
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<s5>09</s5>
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<s2>NM</s2>
<s5>14</s5>
</fC03>
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<s5>14</s5>
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<s2>NW</s2>
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<s2>NW</s2>
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<s2>NW</s2>
</fC07>
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<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
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<s2>NW</s2>
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<s2>NW</s2>
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<s2>NW</s2>
</fC07>
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<s5>13</s5>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Respiratory disease</s0>
<s5>13</s5>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Aparato respiratorio patología</s0>
<s5>13</s5>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Virose</s0>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Viral disease</s0>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Virosis</s0>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Infection</s0>
</fC07>
<fC07 i1="06" i2="X" l="ENG"><s0>Infection</s0>
</fC07>
<fC07 i1="06" i2="X" l="SPA"><s0>Infección</s0>
</fC07>
<fC07 i1="07" i2="X" l="FRE"><s0>Poumon pathologie</s0>
<s5>16</s5>
</fC07>
<fC07 i1="07" i2="X" l="ENG"><s0>Lung disease</s0>
<s5>16</s5>
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<fC07 i1="07" i2="X" l="SPA"><s0>Pulmón patología</s0>
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<server><NO>PASCAL 05-0484475 INIST</NO>
<ET>Recombinant protein-based elisa and immuno-cytochemical assay for the diagnosis of sars</ET>
<AU>CARATTOLI (Alessandra); DI BONITO (Paola); GRASSO (Felicia); GIORGI (Colomba); BLASI (Francesco); NIEDRIG (Matthias); CASSONE (Antonio)</AU>
<AF>Department of Infectious, Parasitic and Immuno-Mediated Diseases, Istituto Superiore di Sanità/Rome/Italie (1 aut., 2 aut., 3 aut., 4 aut., 7 aut.); Ospedale Maggiore Policlinico, University of Milan/Milan/Italie (5 aut.); Robert Koch Institut/Berlin/Allemagne (6 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Journal of medical virology; ISSN 0146-6615; Coden JMVIDB; Etats-Unis; Da. 2005; Vol. 76; No. 2; Pp. 137-142; Bibl. 17 ref.</SO>
<LA>Anglais</LA>
<EA>A new Coronavirus (SARS-CoV) is the aetiological agent of the severe acute respiratory syndrome (SARS). Because of the critical role played by serological assays for SARS diagnosis, an in-house ELISA based on SARS-CoV recombinant antigens was developed. The SARS-CoV nucleocapsid protein (N), three N fragments (N1, N2, and N3) and the intraviral domain of the membrane protein (M2) were cloned and expressed in Escherichia coli as histidine-tagged proteins. Six reference sera from SARS patients were used to detect virus-specific IgG in an ELISA using each recombinant protein as coating antigen. High-titre positive reactions were detected in all SARS positive sera. The specificity of the assay appears to be high as no positive reaction was detected in the sera of 20 healthy subjects and 73 patients with non-SARS, low-tract respiratory infections. Specific hyper-immune sera to SARS-CoV and the recombinant proteins, N, N1, N2, N3, and M2 were also generated in mice and rabbits. The specificity of these sera was confirmed by an immunocytochemical assay on biochips of SARS-CoV infected and uninfected cells.</EA>
<CC>002A05C10; 002B05C02J</CC>
<FD>Coronavirus; Protéine recombinante; Technique ELISA; Diagnostic; Anticorps; Sérologie; Syndrome respiratoire aigu sévère</FD>
<FG>Coronaviridae; Nidovirales; Virus; Appareil respiratoire pathologie; Virose; Infection; Poumon pathologie</FG>
<ED>Coronavirus; Recombinant protein; ELISA assay; Diagnosis; Antibody; Serology; Severe acute respiratory syndrome</ED>
<EG>Coronaviridae; Nidovirales; Virus; Respiratory disease; Viral disease; Infection; Lung disease</EG>
<SD>Coronavirus; Proteína recombinante; Técnica ELISA; Diagnóstico; Anticuerpo; Serología; Síndrome respiratorio agudo severo</SD>
<LO>INIST-17422.354000129811490010</LO>
<ID>05-0484475</ID>
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Recombinant protein-based elisa and immuno-cytochemical assay for the diagnosis of sars

Recombinant protein-based elisa and immuno-cytochemical assay for the diagnosis of sars

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