SrasV1, PascalFrancis, Corpus, bibRecord, 000580

The nsp2 replicase proteins of murine hepatitis virus and severe acute respiratory syndrome coronavirus are dispensable for viral replication

Identifieur interne : 000580 ( PascalFrancis/Corpus ); précédent : 000579; suivant : 000581

The nsp2 replicase proteins of murine hepatitis virus and severe acute respiratory syndrome coronavirus are dispensable for viral replication

Auteurs : Rachel L. Graham ; Amy C. Sims ; Sarah M. Brockway ; Ralph S. Baric ; Mark R. Denison

Source :

Journal of virology [ 0022-538X ] ; 2005.

RBID : Pascal:05-0485339

Descripteurs français

Pascal (Inist)
- Virus hépatite murine, Virus syndrome respiratoire aigu sévère, RNA-directed RNA polymerase, Protéine, Réplication, Microbiologie, Virologie.

English descriptors

KwdEn :
- Microbiology, Murine hepatitis virus, Protein, RNA-directed RNA polymerase, Replication, Severe acute respiratory syndrome virus, Virology.

Abstract

The positive-stranded RNA genome of the coronaviruses is translated from ORF1 to yield polyproteins that are proteolytically processed into intermediate and mature nonstructural proteins (nsps). Murine hepatitis virus (MHV) and severe acute respiratory syndrome coronavirus (SARS-CoV) polyproteins incorporate 16 protein domains (nsps), with nspl and nsp2 being the most variable among the coronaviruses and having no experimentally confirmed or predicted functions in replication. To determine if nsp2 is essential for viral replication, MHV and SARS-CoV genome RNA was generated with deletions of the nsp2 coding sequence (MHVAnsp2 and SARSAnsp2, respectively). Infectious MHVAnsp2 and SARSAnsp2 viruses recovered from electroporated cells had 0.5 to 1 logic reductions in peak titers in single-cycle growth assays, as well as a reduction in viral RNA synthesis that was not specific for any positive-stranded RNA species. The Ansp2 mutant viruses lacked expression of both nsp2 and an nsp2-nsp3 precursor, but cleaved the engineered chimeric nspl-nsp3 cleavage site as efficiently as the native nsp1-nsp2 cleavage site. Replication complexes in MHVAnsp2-infected cells lacked nsp2 but were morphologically indistinguishable from those of wild-type MHV by immunofluorescence. nsp2 expressed in cells by stable retroviral transduction was specifically recruited to viral replication complexes upon infection with MHVAnsp2. These results demonstrate that while nsp2 of MHV and SARS-CoV is dispensable for viral replication in cell culture, deletion of the nsp2 coding sequence attenuates viral growth and RNA synthesis. These findings also provide a system for the study of determinants of nsp targeting and function.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

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A11	`01`	`1`		`@1 GRAHAM (Rachel L.)`
A11	`02`	`1`		`@1 SIMS (Amy C.)`
A11	`03`	`1`		`@1 BROCKWAY (Sarah M.)`
A11	`04`	`1`		`@1 BARIC (Ralph S.)`
A11	`05`	`1`		`@1 DENISON (Mark R.)`
A14	`01`			`@1 Department of Microbiology and Immunology, Vanderbilt University Medical Center @2 Nashville, Tennessee @3 USA @Z 1 aut. @Z 3 aut. @Z 5 aut.`
A14	`02`			`@1 Elizabeth B. Lamb Center for Pediatric Research, Vanderbilt University Medical Center @2 Nashville, Tennessee @3 USA @Z 1 aut. @Z 3 aut. @Z 5 aut.`
A14	`03`			`@1 Department of Epidemiology, School of Public Health, University of North Carolina @2 Chapel Hill, North Carolina @3 USA @Z 2 aut. @Z 4 aut.`
A14	`04`			`@1 Department of Pediatrics, Vanderbilt University Medical Center @2 Nashville, Tennessee @3 USA @Z 5 aut.`
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A44				`@0 0000 @1 © 2005 INIST-CNRS. All rights reserved.`
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C01	`01`		`ENG`	@0 The positive-stranded RNA genome of the coronaviruses is translated from ORF1 to yield polyproteins that are proteolytically processed into intermediate and mature nonstructural proteins (nsps). Murine hepatitis virus (MHV) and severe acute respiratory syndrome coronavirus (SARS-CoV) polyproteins incorporate 16 protein domains (nsps), with nspl and nsp2 being the most variable among the coronaviruses and having no experimentally confirmed or predicted functions in replication. To determine if nsp2 is essential for viral replication, MHV and SARS-CoV genome RNA was generated with deletions of the nsp2 coding sequence (MHVAnsp2 and SARSAnsp2, respectively). Infectious MHVAnsp2 and SARSAnsp2 viruses recovered from electroporated cells had 0.5 to 1 logic reductions in peak titers in single-cycle growth assays, as well as a reduction in viral RNA synthesis that was not specific for any positive-stranded RNA species. The Ansp2 mutant viruses lacked expression of both nsp2 and an nsp2-nsp3 precursor, but cleaved the engineered chimeric nspl-nsp3 cleavage site as efficiently as the native nsp1-nsp2 cleavage site. Replication complexes in MHVAnsp2-infected cells lacked nsp2 but were morphologically indistinguishable from those of wild-type MHV by immunofluorescence. nsp2 expressed in cells by stable retroviral transduction was specifically recruited to viral replication complexes upon infection with MHVAnsp2. These results demonstrate that while nsp2 of MHV and SARS-CoV is dispensable for viral replication in cell culture, deletion of the nsp2 coding sequence attenuates viral growth and RNA synthesis. These findings also provide a system for the study of determinants of nsp targeting and function.
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C03	`01`	`X`	`SPA`	`@0 Murine hepatitis virus @2 NW @5 01`
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C03	`06`	`X`	`ENG`	`@0 Microbiology @5 08`
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C03	`07`	`X`	`SPA`	`@0 Virología @5 09`
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C07	`01`	`X`	`ENG`	`@0 Coronavirus @2 NW`
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C07	`02`	`X`	`ENG`	`@0 Coronaviridae @2 NW`
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C07	`05`	`X`	`SPA`	`@0 Nucleotidyltransferases @2 FE`
C07	`06`	`X`	`FRE`	`@0 Transferases @2 FE`
C07	`06`	`X`	`ENG`	`@0 Transferases @2 FE`
C07	`06`	`X`	`SPA`	`@0 Transferases @2 FE`
C07	`07`	`X`	`FRE`	`@0 Enzyme @2 FE`
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N44	`01`			`@1 OTO`
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Format Inist (serveur)

NO :	PASCAL 05-0485339 INIST
ET :	The nsp2 replicase proteins of murine hepatitis virus and severe acute respiratory syndrome coronavirus are dispensable for viral replication
AU :	GRAHAM (Rachel L.); SIMS (Amy C.); BROCKWAY (Sarah M.); BARIC (Ralph S.); DENISON (Mark R.)
AF :	Department of Microbiology and Immunology, Vanderbilt University Medical Center/Nashville, Tennessee/Etats-Unis (1 aut., 3 aut., 5 aut.); Elizabeth B. Lamb Center for Pediatric Research, Vanderbilt University Medical Center/Nashville, Tennessee/Etats-Unis (1 aut., 3 aut., 5 aut.); Department of Epidemiology, School of Public Health, University of North Carolina/Chapel Hill, North Carolina/Etats-Unis (2 aut., 4 aut.); Department of Pediatrics, Vanderbilt University Medical Center/Nashville, Tennessee/Etats-Unis (5 aut.)
DT :	Publication en série; Niveau analytique
SO :	Journal of virology; ISSN 0022-538X; Etats-Unis; Da. 2005; Vol. 79; No. 21; Pp. 13399-13411; Bibl. 54 ref.
LA :	Anglais
EA :	The positive-stranded RNA genome of the coronaviruses is translated from ORF1 to yield polyproteins that are proteolytically processed into intermediate and mature nonstructural proteins (nsps). Murine hepatitis virus (MHV) and severe acute respiratory syndrome coronavirus (SARS-CoV) polyproteins incorporate 16 protein domains (nsps), with nspl and nsp2 being the most variable among the coronaviruses and having no experimentally confirmed or predicted functions in replication. To determine if nsp2 is essential for viral replication, MHV and SARS-CoV genome RNA was generated with deletions of the nsp2 coding sequence (MHVAnsp2 and SARSAnsp2, respectively). Infectious MHVAnsp2 and SARSAnsp2 viruses recovered from electroporated cells had 0.5 to 1 logic reductions in peak titers in single-cycle growth assays, as well as a reduction in viral RNA synthesis that was not specific for any positive-stranded RNA species. The Ansp2 mutant viruses lacked expression of both nsp2 and an nsp2-nsp3 precursor, but cleaved the engineered chimeric nspl-nsp3 cleavage site as efficiently as the native nsp1-nsp2 cleavage site. Replication complexes in MHVAnsp2-infected cells lacked nsp2 but were morphologically indistinguishable from those of wild-type MHV by immunofluorescence. nsp2 expressed in cells by stable retroviral transduction was specifically recruited to viral replication complexes upon infection with MHVAnsp2. These results demonstrate that while nsp2 of MHV and SARS-CoV is dispensable for viral replication in cell culture, deletion of the nsp2 coding sequence attenuates viral growth and RNA synthesis. These findings also provide a system for the study of determinants of nsp targeting and function.
CC :	002A05C10
FD :	Virus hépatite murine; Virus syndrome respiratoire aigu sévère; RNA-directed RNA polymerase; Protéine; Réplication; Microbiologie; Virologie
FG :	Coronavirus; Coronaviridae; Nidovirales; Virus; Nucleotidyltransferases; Transferases; Enzyme
ED :	Murine hepatitis virus; Severe acute respiratory syndrome virus; RNA-directed RNA polymerase; Protein; Replication; Microbiology; Virology
EG :	Coronavirus; Coronaviridae; Nidovirales; Virus; Nucleotidyltransferases; Transferases; Enzyme
SD :	Murine hepatitis virus; Severe acute respiratory syndrome virus; RNA-directed RNA polymerase; Proteína; Replicación; Microbiología; Virología
LO :	INIST-13592.354000131986280190
ID :	05-0485339

Links to Exploration step

Pascal:05-0485339

Le document en format XML

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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Microbiology</term>
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<term>Severe acute respiratory syndrome virus</term>
<term>Virology</term>
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<keywords scheme="Pascal" xml:lang="fr"><term>Virus hépatite murine</term>
<term>Virus syndrome respiratoire aigu sévère</term>
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<front><div type="abstract" xml:lang="en">The positive-stranded RNA genome of the coronaviruses is translated from ORF1 to yield polyproteins that are proteolytically processed into intermediate and mature nonstructural proteins (nsps). Murine hepatitis virus (MHV) and severe acute respiratory syndrome coronavirus (SARS-CoV) polyproteins incorporate 16 protein domains (nsps), with nspl and nsp2 being the most variable among the coronaviruses and having no experimentally confirmed or predicted functions in replication. To determine if nsp2 is essential for viral replication, MHV and SARS-CoV genome RNA was generated with deletions of the nsp2 coding sequence (MHVAnsp2 and SARSAnsp2, respectively). Infectious MHVAnsp2 and SARSAnsp2 viruses recovered from electroporated cells had 0.5 to 1 logic reductions in peak titers in single-cycle growth assays, as well as a reduction in viral RNA synthesis that was not specific for any positive-stranded RNA species. The Ansp2 mutant viruses lacked expression of both nsp2 and an nsp2-nsp3 precursor, but cleaved the engineered chimeric nspl-nsp3 cleavage site as efficiently as the native nsp1-nsp2 cleavage site. Replication complexes in MHVAnsp2-infected cells lacked nsp2 but were morphologically indistinguishable from those of wild-type MHV by immunofluorescence. nsp2 expressed in cells by stable retroviral transduction was specifically recruited to viral replication complexes upon infection with MHVAnsp2. These results demonstrate that while nsp2 of MHV and SARS-CoV is dispensable for viral replication in cell culture, deletion of the nsp2 coding sequence attenuates viral growth and RNA synthesis. These findings also provide a system for the study of determinants of nsp targeting and function.</div>
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<fA08 i1="01" i2="1" l="ENG"><s1>The nsp2 replicase proteins of murine hepatitis virus and severe acute respiratory syndrome coronavirus are dispensable for viral replication</s1>
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<fA11 i1="01" i2="1"><s1>GRAHAM (Rachel L.)</s1>
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<fA11 i1="02" i2="1"><s1>SIMS (Amy C.)</s1>
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<fA11 i1="03" i2="1"><s1>BROCKWAY (Sarah M.)</s1>
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<fA11 i1="04" i2="1"><s1>BARIC (Ralph S.)</s1>
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<fA11 i1="05" i2="1"><s1>DENISON (Mark R.)</s1>
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<fA14 i1="01"><s1>Department of Microbiology and Immunology, Vanderbilt University Medical Center</s1>
<s2>Nashville, Tennessee</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>5 aut.</sZ>
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<fA14 i1="02"><s1>Elizabeth B. Lamb Center for Pediatric Research, Vanderbilt University Medical Center</s1>
<s2>Nashville, Tennessee</s2>
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<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>5 aut.</sZ>
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<fA14 i1="03"><s1>Department of Epidemiology, School of Public Health, University of North Carolina</s1>
<s2>Chapel Hill, North Carolina</s2>
<s3>USA</s3>
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<sZ>4 aut.</sZ>
</fA14>
<fA14 i1="04"><s1>Department of Pediatrics, Vanderbilt University Medical Center</s1>
<s2>Nashville, Tennessee</s2>
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<sZ>5 aut.</sZ>
</fA14>
<fA20><s1>13399-13411</s1>
</fA20>
<fA21><s1>2005</s1>
</fA21>
<fA23 i1="01"><s0>ENG</s0>
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<fA43 i1="01"><s1>INIST</s1>
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<fA44><s0>0000</s0>
<s1>© 2005 INIST-CNRS. All rights reserved.</s1>
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<fA45><s0>54 ref.</s0>
</fA45>
<fA47 i1="01" i2="1"><s0>05-0485339</s0>
</fA47>
<fA60><s1>P</s1>
</fA60>
<fA61><s0>A</s0>
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<fA64 i1="01" i2="1"><s0>Journal of virology</s0>
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<fA66 i1="01"><s0>USA</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>The positive-stranded RNA genome of the coronaviruses is translated from ORF1 to yield polyproteins that are proteolytically processed into intermediate and mature nonstructural proteins (nsps). Murine hepatitis virus (MHV) and severe acute respiratory syndrome coronavirus (SARS-CoV) polyproteins incorporate 16 protein domains (nsps), with nspl and nsp2 being the most variable among the coronaviruses and having no experimentally confirmed or predicted functions in replication. To determine if nsp2 is essential for viral replication, MHV and SARS-CoV genome RNA was generated with deletions of the nsp2 coding sequence (MHVAnsp2 and SARSAnsp2, respectively). Infectious MHVAnsp2 and SARSAnsp2 viruses recovered from electroporated cells had 0.5 to 1 logic reductions in peak titers in single-cycle growth assays, as well as a reduction in viral RNA synthesis that was not specific for any positive-stranded RNA species. The Ansp2 mutant viruses lacked expression of both nsp2 and an nsp2-nsp3 precursor, but cleaved the engineered chimeric nspl-nsp3 cleavage site as efficiently as the native nsp1-nsp2 cleavage site. Replication complexes in MHVAnsp2-infected cells lacked nsp2 but were morphologically indistinguishable from those of wild-type MHV by immunofluorescence. nsp2 expressed in cells by stable retroviral transduction was specifically recruited to viral replication complexes upon infection with MHVAnsp2. These results demonstrate that while nsp2 of MHV and SARS-CoV is dispensable for viral replication in cell culture, deletion of the nsp2 coding sequence attenuates viral growth and RNA synthesis. These findings also provide a system for the study of determinants of nsp targeting and function.</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>002A05C10</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Virus hépatite murine</s0>
<s2>NW</s2>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>Murine hepatitis virus</s0>
<s2>NW</s2>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Murine hepatitis virus</s0>
<s2>NW</s2>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Virus syndrome respiratoire aigu sévère</s0>
<s2>NW</s2>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Severe acute respiratory syndrome virus</s0>
<s2>NW</s2>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Severe acute respiratory syndrome virus</s0>
<s2>NW</s2>
<s5>02</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>RNA-directed RNA polymerase</s0>
<s2>FE</s2>
<s5>05</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>RNA-directed RNA polymerase</s0>
<s2>FE</s2>
<s5>05</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>RNA-directed RNA polymerase</s0>
<s2>FE</s2>
<s5>05</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Protéine</s0>
<s5>06</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Protein</s0>
<s5>06</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Proteína</s0>
<s5>06</s5>
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<fC03 i1="05" i2="X" l="FRE"><s0>Réplication</s0>
<s5>07</s5>
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<s5>07</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Replicación</s0>
<s5>07</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Microbiologie</s0>
<s5>08</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Microbiology</s0>
<s5>08</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Microbiología</s0>
<s5>08</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE"><s0>Virologie</s0>
<s5>09</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG"><s0>Virology</s0>
<s5>09</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA"><s0>Virología</s0>
<s5>09</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Coronavirus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Coronavirus</s0>
<s2>NW</s2>
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<s2>NW</s2>
</fC07>
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<s2>NW</s2>
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<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Nucleotidyltransferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Nucleotidyltransferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Nucleotidyltransferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Transferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="06" i2="X" l="ENG"><s0>Transferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="06" i2="X" l="SPA"><s0>Transferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="07" i2="X" l="FRE"><s0>Enzyme</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="07" i2="X" l="ENG"><s0>Enzyme</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="07" i2="X" l="SPA"><s0>Enzima</s0>
<s2>FE</s2>
</fC07>
<fN21><s1>339</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
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<server><NO>PASCAL 05-0485339 INIST</NO>
<ET>The nsp2 replicase proteins of murine hepatitis virus and severe acute respiratory syndrome coronavirus are dispensable for viral replication</ET>
<AU>GRAHAM (Rachel L.); SIMS (Amy C.); BROCKWAY (Sarah M.); BARIC (Ralph S.); DENISON (Mark R.)</AU>
<AF>Department of Microbiology and Immunology, Vanderbilt University Medical Center/Nashville, Tennessee/Etats-Unis (1 aut., 3 aut., 5 aut.); Elizabeth B. Lamb Center for Pediatric Research, Vanderbilt University Medical Center/Nashville, Tennessee/Etats-Unis (1 aut., 3 aut., 5 aut.); Department of Epidemiology, School of Public Health, University of North Carolina/Chapel Hill, North Carolina/Etats-Unis (2 aut., 4 aut.); Department of Pediatrics, Vanderbilt University Medical Center/Nashville, Tennessee/Etats-Unis (5 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Journal of virology; ISSN 0022-538X; Etats-Unis; Da. 2005; Vol. 79; No. 21; Pp. 13399-13411; Bibl. 54 ref.</SO>
<LA>Anglais</LA>
<EA>The positive-stranded RNA genome of the coronaviruses is translated from ORF1 to yield polyproteins that are proteolytically processed into intermediate and mature nonstructural proteins (nsps). Murine hepatitis virus (MHV) and severe acute respiratory syndrome coronavirus (SARS-CoV) polyproteins incorporate 16 protein domains (nsps), with nspl and nsp2 being the most variable among the coronaviruses and having no experimentally confirmed or predicted functions in replication. To determine if nsp2 is essential for viral replication, MHV and SARS-CoV genome RNA was generated with deletions of the nsp2 coding sequence (MHVAnsp2 and SARSAnsp2, respectively). Infectious MHVAnsp2 and SARSAnsp2 viruses recovered from electroporated cells had 0.5 to 1 logic reductions in peak titers in single-cycle growth assays, as well as a reduction in viral RNA synthesis that was not specific for any positive-stranded RNA species. The Ansp2 mutant viruses lacked expression of both nsp2 and an nsp2-nsp3 precursor, but cleaved the engineered chimeric nspl-nsp3 cleavage site as efficiently as the native nsp1-nsp2 cleavage site. Replication complexes in MHVAnsp2-infected cells lacked nsp2 but were morphologically indistinguishable from those of wild-type MHV by immunofluorescence. nsp2 expressed in cells by stable retroviral transduction was specifically recruited to viral replication complexes upon infection with MHVAnsp2. These results demonstrate that while nsp2 of MHV and SARS-CoV is dispensable for viral replication in cell culture, deletion of the nsp2 coding sequence attenuates viral growth and RNA synthesis. These findings also provide a system for the study of determinants of nsp targeting and function.</EA>
<CC>002A05C10</CC>
<FD>Virus hépatite murine; Virus syndrome respiratoire aigu sévère; RNA-directed RNA polymerase; Protéine; Réplication; Microbiologie; Virologie</FD>
<FG>Coronavirus; Coronaviridae; Nidovirales; Virus; Nucleotidyltransferases; Transferases; Enzyme</FG>
<ED>Murine hepatitis virus; Severe acute respiratory syndrome virus; RNA-directed RNA polymerase; Protein; Replication; Microbiology; Virology</ED>
<EG>Coronavirus; Coronaviridae; Nidovirales; Virus; Nucleotidyltransferases; Transferases; Enzyme</EG>
<SD>Murine hepatitis virus; Severe acute respiratory syndrome virus; RNA-directed RNA polymerase; Proteína; Replicación; Microbiología; Virología</SD>
<LO>INIST-13592.354000131986280190</LO>
<ID>05-0485339</ID>
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The nsp2 replicase proteins of murine hepatitis virus and severe acute respiratory syndrome coronavirus are dispensable for viral replication

The nsp2 replicase proteins of murine hepatitis virus and severe acute respiratory syndrome coronavirus are dispensable for viral replication

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