SrasV1, PascalFrancis, Corpus, bibRecord, 000565

Subcellular localization of the severe acute respiratory syndrome coronavirus nucleocapsid protein

Identifieur interne : 000565 ( PascalFrancis/Corpus ); précédent : 000564; suivant : 000566

Subcellular localization of the severe acute respiratory syndrome coronavirus nucleocapsid protein

Auteurs : Jaehwan You ; Brian K. Dove ; Luis Enjuanes ; Marta L. Dediego ; Enrique Alvarez ; Gareth Howell ; Paul Heinen ; Maria Zambon ; Julian A. Hiscox

Source :

Journal of general virology [ 0022-1317 ] ; 2005.

RBID : Pascal:06-0028823

Descripteurs français

Pascal (Inist)
- Coronavirus, Localisation, Nucléocapside, Protéine, Microbiologie, Virologie, Syndrome respiratoire aigu sévère.

English descriptors

KwdEn :
- Coronavirus, Localization, Microbiology, Nucleocapsid, Protein, Severe acute respiratory syndrome, Virology.

Abstract

The coronavirus nucleocapsid (N) protein is a viral RNA-binding protein with multiple functions in terms of virus replication and modulating cell signalling pathways. N protein is composed of three distinct regions containing RNA-binding motif(s), and appropriate signals for modulating cell signalling. The subcellular localization of severe acute respiratory syndrome coronavirus (SARS-CoV) N protein was studied. In infected cells, SARS-CoV N protein localized exclusively to the cytoplasm. In contrast to the avian coronavirus N protein, overexpressed SARS-CoV N protein remained principally localized to the cytoplasm, with very few cells exhibiting nucleolar localization. Bioinformatic analysis and deletion mutagenesis coupled to confocal microscopy and live-cell imaging, revealed that SARS-CoV N protein regions I and III contained nuclear localization signals and region II contained a nucleolar retention signal. However, cytoplasmic localization was directed by region III and was the dominant localization signal in the protein.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

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A11	`02`	`1`		`@1 DOVE (Brian K.)`
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A14	`03`			`@1 Astbury Centre for Structural Molecular Biology, University of Leeds @2 Leeds LS2 9JT @3 GBR @Z 6 aut. @Z 9 aut.`
A14	`04`			`@1 Health Protection Agency @2 London NW9 5HT @3 GBR @Z 7 aut. @Z 8 aut.`
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C01	`01`		`ENG`	@0 The coronavirus nucleocapsid (N) protein is a viral RNA-binding protein with multiple functions in terms of virus replication and modulating cell signalling pathways. N protein is composed of three distinct regions containing RNA-binding motif(s), and appropriate signals for modulating cell signalling. The subcellular localization of severe acute respiratory syndrome coronavirus (SARS-CoV) N protein was studied. In infected cells, SARS-CoV N protein localized exclusively to the cytoplasm. In contrast to the avian coronavirus N protein, overexpressed SARS-CoV N protein remained principally localized to the cytoplasm, with very few cells exhibiting nucleolar localization. Bioinformatic analysis and deletion mutagenesis coupled to confocal microscopy and live-cell imaging, revealed that SARS-CoV N protein regions I and III contained nuclear localization signals and region II contained a nucleolar retention signal. However, cytoplasmic localization was directed by region III and was the dominant localization signal in the protein.
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Format Inist (serveur)

NO :	PASCAL 06-0028823 INIST
ET :	Subcellular localization of the severe acute respiratory syndrome coronavirus nucleocapsid protein
AU :	YOU (Jaehwan); DOVE (Brian K.); ENJUANES (Luis); DEDIEGO (Marta L.); ALVAREZ (Enrique); HOWELL (Gareth); HEINEN (Paul); ZAMBON (Maria); HISCOX (Julian A.)
AF :	Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, Garstang Building, University of Leeds/Leeds LS2 9JT/Royaume-Uni (1 aut., 2 aut., 9 aut.); Department of Molecular and Cell Biology, Centro Nacional de Biotecnología (CNB, CSIC), Campus Univ. Autonoma, 3 Darwin Street/Cantoblanco, 28049 Madrid/Espagne (3 aut., 4 aut., 5 aut.); Astbury Centre for Structural Molecular Biology, University of Leeds/Leeds LS2 9JT/Royaume-Uni (6 aut., 9 aut.); Health Protection Agency/London NW9 5HT/Royaume-Uni (7 aut., 8 aut.)
DT :	Publication en série; Courte communication, note brève; Niveau analytique
SO :	Journal of general virology; ISSN 0022-1317; Coden JGVIAY; Royaume-Uni; Da. 2005; Vol. 86; No. p.12; Pp. 3303-3310; Bibl. 39 ref.
LA :	Anglais
EA :	The coronavirus nucleocapsid (N) protein is a viral RNA-binding protein with multiple functions in terms of virus replication and modulating cell signalling pathways. N protein is composed of three distinct regions containing RNA-binding motif(s), and appropriate signals for modulating cell signalling. The subcellular localization of severe acute respiratory syndrome coronavirus (SARS-CoV) N protein was studied. In infected cells, SARS-CoV N protein localized exclusively to the cytoplasm. In contrast to the avian coronavirus N protein, overexpressed SARS-CoV N protein remained principally localized to the cytoplasm, with very few cells exhibiting nucleolar localization. Bioinformatic analysis and deletion mutagenesis coupled to confocal microscopy and live-cell imaging, revealed that SARS-CoV N protein regions I and III contained nuclear localization signals and region II contained a nucleolar retention signal. However, cytoplasmic localization was directed by region III and was the dominant localization signal in the protein.
CC :	002A05C10
FD :	Coronavirus; Localisation; Nucléocapside; Protéine; Microbiologie; Virologie; Syndrome respiratoire aigu sévère
FG :	Coronaviridae; Nidovirales; Virus; Appareil respiratoire pathologie; Virose; Infection; Poumon pathologie
ED :	Coronavirus; Localization; Nucleocapsid; Protein; Microbiology; Virology; Severe acute respiratory syndrome
EG :	Coronaviridae; Nidovirales; Virus; Respiratory disease; Viral disease; Infection; Lung disease
SD :	Coronavirus; Localización; Nucleocápside; Proteína; Microbiología; Virología; Síndrome respiratorio agudo severo
LO :	INIST-13533.354000135461720130
ID :	06-0028823

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Pascal:06-0028823

Le document en format XML

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<series><title level="j" type="main">Journal of general virology</title>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Coronavirus</term>
<term>Localization</term>
<term>Microbiology</term>
<term>Nucleocapsid</term>
<term>Protein</term>
<term>Severe acute respiratory syndrome</term>
<term>Virology</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Coronavirus</term>
<term>Localisation</term>
<term>Nucléocapside</term>
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<term>Virologie</term>
<term>Syndrome respiratoire aigu sévère</term>
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<front><div type="abstract" xml:lang="en">The coronavirus nucleocapsid (N) protein is a viral RNA-binding protein with multiple functions in terms of virus replication and modulating cell signalling pathways. N protein is composed of three distinct regions containing RNA-binding motif(s), and appropriate signals for modulating cell signalling. The subcellular localization of severe acute respiratory syndrome coronavirus (SARS-CoV) N protein was studied. In infected cells, SARS-CoV N protein localized exclusively to the cytoplasm. In contrast to the avian coronavirus N protein, overexpressed SARS-CoV N protein remained principally localized to the cytoplasm, with very few cells exhibiting nucleolar localization. Bioinformatic analysis and deletion mutagenesis coupled to confocal microscopy and live-cell imaging, revealed that SARS-CoV N protein regions I and III contained nuclear localization signals and region II contained a nucleolar retention signal. However, cytoplasmic localization was directed by region III and was the dominant localization signal in the protein.</div>
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<sZ>9 aut.</sZ>
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<sZ>4 aut.</sZ>
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<sZ>6 aut.</sZ>
<sZ>9 aut.</sZ>
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<s3>GBR</s3>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
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<s2>NW</s2>
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<fN21><s1>009</s1>
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<server><NO>PASCAL 06-0028823 INIST</NO>
<ET>Subcellular localization of the severe acute respiratory syndrome coronavirus nucleocapsid protein</ET>
<AU>YOU (Jaehwan); DOVE (Brian K.); ENJUANES (Luis); DEDIEGO (Marta L.); ALVAREZ (Enrique); HOWELL (Gareth); HEINEN (Paul); ZAMBON (Maria); HISCOX (Julian A.)</AU>
<AF>Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, Garstang Building, University of Leeds/Leeds LS2 9JT/Royaume-Uni (1 aut., 2 aut., 9 aut.); Department of Molecular and Cell Biology, Centro Nacional de Biotecnología (CNB, CSIC), Campus Univ. Autonoma, 3 Darwin Street/Cantoblanco, 28049 Madrid/Espagne (3 aut., 4 aut., 5 aut.); Astbury Centre for Structural Molecular Biology, University of Leeds/Leeds LS2 9JT/Royaume-Uni (6 aut., 9 aut.); Health Protection Agency/London NW9 5HT/Royaume-Uni (7 aut., 8 aut.)</AF>
<DT>Publication en série; Courte communication, note brève; Niveau analytique</DT>
<SO>Journal of general virology; ISSN 0022-1317; Coden JGVIAY; Royaume-Uni; Da. 2005; Vol. 86; No. p.12; Pp. 3303-3310; Bibl. 39 ref.</SO>
<LA>Anglais</LA>
<EA>The coronavirus nucleocapsid (N) protein is a viral RNA-binding protein with multiple functions in terms of virus replication and modulating cell signalling pathways. N protein is composed of three distinct regions containing RNA-binding motif(s), and appropriate signals for modulating cell signalling. The subcellular localization of severe acute respiratory syndrome coronavirus (SARS-CoV) N protein was studied. In infected cells, SARS-CoV N protein localized exclusively to the cytoplasm. In contrast to the avian coronavirus N protein, overexpressed SARS-CoV N protein remained principally localized to the cytoplasm, with very few cells exhibiting nucleolar localization. Bioinformatic analysis and deletion mutagenesis coupled to confocal microscopy and live-cell imaging, revealed that SARS-CoV N protein regions I and III contained nuclear localization signals and region II contained a nucleolar retention signal. However, cytoplasmic localization was directed by region III and was the dominant localization signal in the protein.</EA>
<CC>002A05C10</CC>
<FD>Coronavirus; Localisation; Nucléocapside; Protéine; Microbiologie; Virologie; Syndrome respiratoire aigu sévère</FD>
<FG>Coronaviridae; Nidovirales; Virus; Appareil respiratoire pathologie; Virose; Infection; Poumon pathologie</FG>
<ED>Coronavirus; Localization; Nucleocapsid; Protein; Microbiology; Virology; Severe acute respiratory syndrome</ED>
<EG>Coronaviridae; Nidovirales; Virus; Respiratory disease; Viral disease; Infection; Lung disease</EG>
<SD>Coronavirus; Localización; Nucleocápside; Proteína; Microbiología; Virología; Síndrome respiratorio agudo severo</SD>
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Subcellular localization of the severe acute respiratory syndrome coronavirus nucleocapsid protein

Subcellular localization of the severe acute respiratory syndrome coronavirus nucleocapsid protein

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