SrasV1, PascalFrancis, Corpus, bibRecord, 000402

Lipid rafts play an important role in the early stage of severe acute respiratory syndrome-coronavirus life cycle

Identifieur interne : 000402 ( PascalFrancis/Corpus ); précédent : 000401; suivant : 000403

Lipid rafts play an important role in the early stage of severe acute respiratory syndrome-coronavirus life cycle

Auteurs : Gui-Mei Li ; Yong-Gang Li ; Masanobu Yamate ; Shu-Ming Li ; Kazuyoshi Ikuta

Source :

Microbes and infection [ 1286-4579 ] ; 2007.

RBID : Pascal:07-0134374

Descripteurs français

Pascal (Inist)
- Coronavirus, Stade précoce, Cycle développement, Syndrome respiratoire aigu sévère, Microdomaine de type raft.

English descriptors

KwdEn :
- Coronavirus, Early stage, Life cycle, Lipid rafts, Severe acute respiratory syndrome.

Abstract

Lipid rafts are involved in the life cycle of many viruses. In this study, we showed that lipid rafts also play an important role in the life cycle of severe acute respiratory syndrome (SARS)-coronavirus (CoV). Cholesterol depletion by pretreatment of Vero E6 cells with methyl-β-cyclodextrin (MβCD) inhibited the production of SARS-CoV particles released from the infected cells. This inhibition was prevented by addition of cholesterol to the culture medium, indicating that the reduction of virus particle release was caused by the loss of cholesterol in the cell membrane. In contrast, cholesterol depletion at the post-entry stage (3 h post-infection) caused only a limited effect on virus particle release. Northern blot analysis revealed that the levels of viral mRNAs were significantly affected by pretreatment with MpCD, but not by treatment at 3 h post-infection. Interestingly, no apparent evidence for colocalization of angiotensin converting enzyme 2 with lipid rafts in the membrane of Vero E6 cells was obtained. These results suggest that lipid rafts could contribute to SARS-CoV infection in the early replication process in Vero E6 cells.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

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A11	`02`	`1`		`@1 LI (Yong-Gang)`
A11	`03`	`1`		`@1 YAMATE (Masanobu)`
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A14	`01`			`@1 Department of Virology, Research Institute for Microhial Diseases (RIMD), Osaka University @2 Suita, Osaka 565-0871 @3 JPN @Z 1 aut. @Z 2 aut. @Z 3 aut. @Z 4 aut. @Z 5 aut.`
A14	`02`			`@1 Research Collaboration Center on Emerging and Re-emerging Infections (RCC-ERI) of National Institute of Health-Department of Medical Sciences and RIMD-Osaka University @2 Nonthaburi 11000 @3 THA @Z 2 aut. @Z 5 aut.`
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A44				`@0 0000 @1 © 2007 INIST-CNRS. All rights reserved.`
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C01	`01`		`ENG`	@0 Lipid rafts are involved in the life cycle of many viruses. In this study, we showed that lipid rafts also play an important role in the life cycle of severe acute respiratory syndrome (SARS)-coronavirus (CoV). Cholesterol depletion by pretreatment of Vero E6 cells with methyl-β-cyclodextrin (MβCD) inhibited the production of SARS-CoV particles released from the infected cells. This inhibition was prevented by addition of cholesterol to the culture medium, indicating that the reduction of virus particle release was caused by the loss of cholesterol in the cell membrane. In contrast, cholesterol depletion at the post-entry stage (3 h post-infection) caused only a limited effect on virus particle release. Northern blot analysis revealed that the levels of viral mRNAs were significantly affected by pretreatment with MpCD, but not by treatment at 3 h post-infection. Interestingly, no apparent evidence for colocalization of angiotensin converting enzyme 2 with lipid rafts in the membrane of Vero E6 cells was obtained. These results suggest that lipid rafts could contribute to SARS-CoV infection in the early replication process in Vero E6 cells.
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Format Inist (serveur)

NO :	PASCAL 07-0134374 INIST
ET :	Lipid rafts play an important role in the early stage of severe acute respiratory syndrome-coronavirus life cycle
AU :	LI (Gui-Mei); LI (Yong-Gang); YAMATE (Masanobu); LI (Shu-Ming); IKUTA (Kazuyoshi)
AF :	Department of Virology, Research Institute for Microhial Diseases (RIMD), Osaka University/Suita, Osaka 565-0871/Japon (1 aut., 2 aut., 3 aut., 4 aut., 5 aut.); Research Collaboration Center on Emerging and Re-emerging Infections (RCC-ERI) of National Institute of Health-Department of Medical Sciences and RIMD-Osaka University/Nonthaburi 11000/Thaïlande (2 aut., 5 aut.)
DT :	Publication en série; Niveau analytique
SO :	Microbes and infection; ISSN 1286-4579; France; Da. 2007; Vol. 9; No. 1; Pp. 96-102; Bibl. 30 ref.
LA :	Anglais
EA :	Lipid rafts are involved in the life cycle of many viruses. In this study, we showed that lipid rafts also play an important role in the life cycle of severe acute respiratory syndrome (SARS)-coronavirus (CoV). Cholesterol depletion by pretreatment of Vero E6 cells with methyl-β-cyclodextrin (MβCD) inhibited the production of SARS-CoV particles released from the infected cells. This inhibition was prevented by addition of cholesterol to the culture medium, indicating that the reduction of virus particle release was caused by the loss of cholesterol in the cell membrane. In contrast, cholesterol depletion at the post-entry stage (3 h post-infection) caused only a limited effect on virus particle release. Northern blot analysis revealed that the levels of viral mRNAs were significantly affected by pretreatment with MpCD, but not by treatment at 3 h post-infection. Interestingly, no apparent evidence for colocalization of angiotensin converting enzyme 2 with lipid rafts in the membrane of Vero E6 cells was obtained. These results suggest that lipid rafts could contribute to SARS-CoV infection in the early replication process in Vero E6 cells.
CC :	002A05C10
FD :	Coronavirus; Stade précoce; Cycle développement; Syndrome respiratoire aigu sévère; Microdomaine de type raft
FG :	Coronaviridae; Nidovirales; Virus; Appareil respiratoire pathologie; Virose; Infection; Poumon pathologie
ED :	Coronavirus; Early stage; Life cycle; Severe acute respiratory syndrome; Lipid rafts
EG :	Coronaviridae; Nidovirales; Virus; Respiratory disease; Viral disease; Infection; Lung disease
SD :	Coronavirus; Estadio precoz; Ciclo desarrollo; Síndrome respiratorio agudo severo
LO :	INIST-26816.354000159747110140
ID :	07-0134374

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Pascal:07-0134374

Le document en format XML

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<front><div type="abstract" xml:lang="en">Lipid rafts are involved in the life cycle of many viruses. In this study, we showed that lipid rafts also play an important role in the life cycle of severe acute respiratory syndrome (SARS)-coronavirus (CoV). Cholesterol depletion by pretreatment of Vero E6 cells with methyl-β-cyclodextrin (MβCD) inhibited the production of SARS-CoV particles released from the infected cells. This inhibition was prevented by addition of cholesterol to the culture medium, indicating that the reduction of virus particle release was caused by the loss of cholesterol in the cell membrane. In contrast, cholesterol depletion at the post-entry stage (3 h post-infection) caused only a limited effect on virus particle release. Northern blot analysis revealed that the levels of viral mRNAs were significantly affected by pretreatment with MpCD, but not by treatment at 3 h post-infection. Interestingly, no apparent evidence for colocalization of angiotensin converting enzyme 2 with lipid rafts in the membrane of Vero E6 cells was obtained. These results suggest that lipid rafts could contribute to SARS-CoV infection in the early replication process in Vero E6 cells.</div>
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<s5>06</s5>
</fC03>
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<s5>06</s5>
</fC03>
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<s5>06</s5>
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<s2>NW</s2>
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<fC07 i1="04" i2="X" l="FRE"><s0>Appareil respiratoire pathologie</s0>
<s5>13</s5>
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<s5>13</s5>
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<s5>13</s5>
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<fC07 i1="05" i2="X" l="ENG"><s0>Viral disease</s0>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Virosis</s0>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Infection</s0>
</fC07>
<fC07 i1="06" i2="X" l="ENG"><s0>Infection</s0>
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<fC07 i1="06" i2="X" l="SPA"><s0>Infección</s0>
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<fC07 i1="07" i2="X" l="FRE"><s0>Poumon pathologie</s0>
<s5>16</s5>
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<s5>16</s5>
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<fC07 i1="07" i2="X" l="SPA"><s0>Pulmón patología</s0>
<s5>16</s5>
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<server><NO>PASCAL 07-0134374 INIST</NO>
<ET>Lipid rafts play an important role in the early stage of severe acute respiratory syndrome-coronavirus life cycle</ET>
<AU>LI (Gui-Mei); LI (Yong-Gang); YAMATE (Masanobu); LI (Shu-Ming); IKUTA (Kazuyoshi)</AU>
<AF>Department of Virology, Research Institute for Microhial Diseases (RIMD), Osaka University/Suita, Osaka 565-0871/Japon (1 aut., 2 aut., 3 aut., 4 aut., 5 aut.); Research Collaboration Center on Emerging and Re-emerging Infections (RCC-ERI) of National Institute of Health-Department of Medical Sciences and RIMD-Osaka University/Nonthaburi 11000/Thaïlande (2 aut., 5 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Microbes and infection; ISSN 1286-4579; France; Da. 2007; Vol. 9; No. 1; Pp. 96-102; Bibl. 30 ref.</SO>
<LA>Anglais</LA>
<EA>Lipid rafts are involved in the life cycle of many viruses. In this study, we showed that lipid rafts also play an important role in the life cycle of severe acute respiratory syndrome (SARS)-coronavirus (CoV). Cholesterol depletion by pretreatment of Vero E6 cells with methyl-β-cyclodextrin (MβCD) inhibited the production of SARS-CoV particles released from the infected cells. This inhibition was prevented by addition of cholesterol to the culture medium, indicating that the reduction of virus particle release was caused by the loss of cholesterol in the cell membrane. In contrast, cholesterol depletion at the post-entry stage (3 h post-infection) caused only a limited effect on virus particle release. Northern blot analysis revealed that the levels of viral mRNAs were significantly affected by pretreatment with MpCD, but not by treatment at 3 h post-infection. Interestingly, no apparent evidence for colocalization of angiotensin converting enzyme 2 with lipid rafts in the membrane of Vero E6 cells was obtained. These results suggest that lipid rafts could contribute to SARS-CoV infection in the early replication process in Vero E6 cells.</EA>
<CC>002A05C10</CC>
<FD>Coronavirus; Stade précoce; Cycle développement; Syndrome respiratoire aigu sévère; Microdomaine de type raft</FD>
<FG>Coronaviridae; Nidovirales; Virus; Appareil respiratoire pathologie; Virose; Infection; Poumon pathologie</FG>
<ED>Coronavirus; Early stage; Life cycle; Severe acute respiratory syndrome; Lipid rafts</ED>
<EG>Coronaviridae; Nidovirales; Virus; Respiratory disease; Viral disease; Infection; Lung disease</EG>
<SD>Coronavirus; Estadio precoz; Ciclo desarrollo; Síndrome respiratorio agudo severo</SD>
<LO>INIST-26816.354000159747110140</LO>
<ID>07-0134374</ID>
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Lipid rafts play an important role in the early stage of severe acute respiratory syndrome-coronavirus life cycle

Lipid rafts play an important role in the early stage of severe acute respiratory syndrome-coronavirus life cycle

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