Lethal infection of K18-hACE2 mice infected with severe acute respiratory syndrome coronavirus
Identifieur interne : 000384 ( PascalFrancis/Corpus ); précédent : 000383; suivant : 000385Lethal infection of K18-hACE2 mice infected with severe acute respiratory syndrome coronavirus
Auteurs : Paul B. Jr Mccray ; Lecia Pewe ; Christine Wohlford-Lenane ; Melissa Hickey ; Lori Manzel ; LEI SHI ; Jason Netland ; HONG PENG JIA ; Carmen Halabi ; Curt D. Sigmund ; David K. Meyerholz ; Patricia Kirby ; Dwight C. Look ; Stanley PerlmanSource :
- Journal of virology [ 0022-538X ] ; 2007.
Descripteurs français
- Pascal (Inist)
English descriptors
Abstract
The severe acute respiratory syndrome (SARS), caused by a novel coronavirus (SARS-CoV), resulted in substantial morbidity, mortality, and economic losses during the 2003 epidemic. While SARS-CoV infection has not recurred to a significant extent since 2003, it still remains a potential threat. Understanding of SARS and development of therapeutic approaches have been hampered by the absence of an animal model that mimics the human disease and is reproducible. Here we show that transgenic mice that express the SARS-CoV receptor (human angiotensin-converting enzyme 2 [hACE2]) in airway and other epithelia develop a rapidly lethal infection after intranasal inoculation with a human strain of the virus. Infection begins in airway epithelia, with subsequent alveolar involvement and extrapulmonary virus spread to the brain. Infection results in macrophage and lymphocyte infiltration in the lungs and upregulation of proinflammatory cytokines and chemokines in both the lung and the brain. This model of lethal infection with SARS-CoV should be useful for studies of pathogenesis and for the development of antiviral therapies.
Notice en format standard (ISO 2709)
Pour connaître la documentation sur le format Inist Standard.
pA |
|
---|
Format Inist (serveur)
NO : | PASCAL 07-0182099 INIST |
---|---|
ET : | Lethal infection of K18-hACE2 mice infected with severe acute respiratory syndrome coronavirus |
AU : | MCCRAY (Paul B. JR); PEWE (Lecia); WOHLFORD-LENANE (Christine); HICKEY (Melissa); MANZEL (Lori); LEI SHI; NETLAND (Jason); HONG PENG JIA; HALABI (Carmen); SIGMUND (Curt D.); MEYERHOLZ (David K.); KIRBY (Patricia); LOOK (Dwight C.); PERLMAN (Stanley) |
AF : | Department of Pediatrics, University of Iowa/Iowa City, Iowa 52242/Etats-Unis (1 aut., 3 aut., 4 aut., 8 aut., 14 aut.); Department of Genetics, University of Iowa/Iowa City, Iowa 52242/Etats-Unis (1 aut., 9 aut., 10 aut.); Department of Microbiology, University of Iowa/Iowa City, Iowa 52242/Etats-Unis (2 aut., 9 aut., 10 aut., 14 aut.); Department of Internal Medicine, University of Iowa/Iowa City, Iowa 52242/Etats-Unis (5 aut., 6 aut., 13 aut.); Department of Interdisciplinary Programs in Immunology, University of Iowa/Iowa City, Iowa 52242/Etats-Unis (7 aut., 14 aut.); Department of Pathology, University of Iowa/Iowa City, Iowa 52242/Etats-Unis (11 aut., 12 aut.) |
DT : | Publication en série; Niveau analytique |
SO : | Journal of virology; ISSN 0022-538X; Etats-Unis; Da. 2007; Vol. 81; No. 2; Pp. 813-821; Bibl. 43 ref. |
LA : | Anglais |
EA : | The severe acute respiratory syndrome (SARS), caused by a novel coronavirus (SARS-CoV), resulted in substantial morbidity, mortality, and economic losses during the 2003 epidemic. While SARS-CoV infection has not recurred to a significant extent since 2003, it still remains a potential threat. Understanding of SARS and development of therapeutic approaches have been hampered by the absence of an animal model that mimics the human disease and is reproducible. Here we show that transgenic mice that express the SARS-CoV receptor (human angiotensin-converting enzyme 2 [hACE2]) in airway and other epithelia develop a rapidly lethal infection after intranasal inoculation with a human strain of the virus. Infection begins in airway epithelia, with subsequent alveolar involvement and extrapulmonary virus spread to the brain. Infection results in macrophage and lymphocyte infiltration in the lungs and upregulation of proinflammatory cytokines and chemokines in both the lung and the brain. This model of lethal infection with SARS-CoV should be useful for studies of pathogenesis and for the development of antiviral therapies. |
CC : | 002A05C10 |
FD : | Souris; Coronavirus; Virologie; Infection; Syndrome respiratoire aigu sévère |
FG : | Rodentia; Mammalia; Vertebrata; Coronaviridae; Nidovirales; Virus; Appareil respiratoire pathologie; Virose; Poumon pathologie |
ED : | Mouse; Coronavirus; Virology; Infection; Severe acute respiratory syndrome |
EG : | Rodentia; Mammalia; Vertebrata; Coronaviridae; Nidovirales; Virus; Respiratory disease; Viral disease; Lung disease |
SD : | Ratón; Coronavirus; Virología; Infección; Síndrome respiratorio agudo severo |
LO : | INIST-13592.354000159387240380 |
ID : | 07-0182099 |
Links to Exploration step
Pascal:07-0182099Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Lethal infection of K18-hACE2 mice infected with severe acute respiratory syndrome coronavirus</title>
<author><name sortKey="Mccray, Paul B Jr" sort="Mccray, Paul B Jr" uniqKey="Mccray P" first="Paul B. Jr" last="Mccray">Paul B. Jr Mccray</name>
<affiliation><inist:fA14 i1="01"><s1>Department of Pediatrics, University of Iowa</s1>
<s2>Iowa City, Iowa 52242</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>14 aut.</sZ>
</inist:fA14>
</affiliation>
<affiliation><inist:fA14 i1="02"><s1>Department of Genetics, University of Iowa</s1>
<s2>Iowa City, Iowa 52242</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Pewe, Lecia" sort="Pewe, Lecia" uniqKey="Pewe L" first="Lecia" last="Pewe">Lecia Pewe</name>
<affiliation><inist:fA14 i1="03"><s1>Department of Microbiology, University of Iowa</s1>
<s2>Iowa City, Iowa 52242</s2>
<s3>USA</s3>
<sZ>2 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>14 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Wohlford Lenane, Christine" sort="Wohlford Lenane, Christine" uniqKey="Wohlford Lenane C" first="Christine" last="Wohlford-Lenane">Christine Wohlford-Lenane</name>
<affiliation><inist:fA14 i1="01"><s1>Department of Pediatrics, University of Iowa</s1>
<s2>Iowa City, Iowa 52242</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>14 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Hickey, Melissa" sort="Hickey, Melissa" uniqKey="Hickey M" first="Melissa" last="Hickey">Melissa Hickey</name>
<affiliation><inist:fA14 i1="01"><s1>Department of Pediatrics, University of Iowa</s1>
<s2>Iowa City, Iowa 52242</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>14 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Manzel, Lori" sort="Manzel, Lori" uniqKey="Manzel L" first="Lori" last="Manzel">Lori Manzel</name>
<affiliation><inist:fA14 i1="04"><s1>Department of Internal Medicine, University of Iowa</s1>
<s2>Iowa City, Iowa 52242</s2>
<s3>USA</s3>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>13 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Lei Shi" sort="Lei Shi" uniqKey="Lei Shi" last="Lei Shi">LEI SHI</name>
<affiliation><inist:fA14 i1="04"><s1>Department of Internal Medicine, University of Iowa</s1>
<s2>Iowa City, Iowa 52242</s2>
<s3>USA</s3>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>13 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Netland, Jason" sort="Netland, Jason" uniqKey="Netland J" first="Jason" last="Netland">Jason Netland</name>
<affiliation><inist:fA14 i1="05"><s1>Department of Interdisciplinary Programs in Immunology, University of Iowa</s1>
<s2>Iowa City, Iowa 52242</s2>
<s3>USA</s3>
<sZ>7 aut.</sZ>
<sZ>14 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Hong Peng Jia" sort="Hong Peng Jia" uniqKey="Hong Peng Jia" last="Hong Peng Jia">HONG PENG JIA</name>
<affiliation><inist:fA14 i1="01"><s1>Department of Pediatrics, University of Iowa</s1>
<s2>Iowa City, Iowa 52242</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>14 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Halabi, Carmen" sort="Halabi, Carmen" uniqKey="Halabi C" first="Carmen" last="Halabi">Carmen Halabi</name>
<affiliation><inist:fA14 i1="02"><s1>Department of Genetics, University of Iowa</s1>
<s2>Iowa City, Iowa 52242</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
</affiliation>
<affiliation><inist:fA14 i1="03"><s1>Department of Microbiology, University of Iowa</s1>
<s2>Iowa City, Iowa 52242</s2>
<s3>USA</s3>
<sZ>2 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>14 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Sigmund, Curt D" sort="Sigmund, Curt D" uniqKey="Sigmund C" first="Curt D." last="Sigmund">Curt D. Sigmund</name>
<affiliation><inist:fA14 i1="02"><s1>Department of Genetics, University of Iowa</s1>
<s2>Iowa City, Iowa 52242</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
</affiliation>
<affiliation><inist:fA14 i1="03"><s1>Department of Microbiology, University of Iowa</s1>
<s2>Iowa City, Iowa 52242</s2>
<s3>USA</s3>
<sZ>2 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>14 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Meyerholz, David K" sort="Meyerholz, David K" uniqKey="Meyerholz D" first="David K." last="Meyerholz">David K. Meyerholz</name>
<affiliation><inist:fA14 i1="06"><s1>Department of Pathology, University of Iowa</s1>
<s2>Iowa City, Iowa 52242</s2>
<s3>USA</s3>
<sZ>11 aut.</sZ>
<sZ>12 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Kirby, Patricia" sort="Kirby, Patricia" uniqKey="Kirby P" first="Patricia" last="Kirby">Patricia Kirby</name>
<affiliation><inist:fA14 i1="06"><s1>Department of Pathology, University of Iowa</s1>
<s2>Iowa City, Iowa 52242</s2>
<s3>USA</s3>
<sZ>11 aut.</sZ>
<sZ>12 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Look, Dwight C" sort="Look, Dwight C" uniqKey="Look D" first="Dwight C." last="Look">Dwight C. Look</name>
<affiliation><inist:fA14 i1="04"><s1>Department of Internal Medicine, University of Iowa</s1>
<s2>Iowa City, Iowa 52242</s2>
<s3>USA</s3>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>13 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Perlman, Stanley" sort="Perlman, Stanley" uniqKey="Perlman S" first="Stanley" last="Perlman">Stanley Perlman</name>
<affiliation><inist:fA14 i1="01"><s1>Department of Pediatrics, University of Iowa</s1>
<s2>Iowa City, Iowa 52242</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>14 aut.</sZ>
</inist:fA14>
</affiliation>
<affiliation><inist:fA14 i1="03"><s1>Department of Microbiology, University of Iowa</s1>
<s2>Iowa City, Iowa 52242</s2>
<s3>USA</s3>
<sZ>2 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>14 aut.</sZ>
</inist:fA14>
</affiliation>
<affiliation><inist:fA14 i1="05"><s1>Department of Interdisciplinary Programs in Immunology, University of Iowa</s1>
<s2>Iowa City, Iowa 52242</s2>
<s3>USA</s3>
<sZ>7 aut.</sZ>
<sZ>14 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">07-0182099</idno>
<date when="2007">2007</date>
<idno type="stanalyst">PASCAL 07-0182099 INIST</idno>
<idno type="RBID">Pascal:07-0182099</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000384</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Lethal infection of K18-hACE2 mice infected with severe acute respiratory syndrome coronavirus</title>
<author><name sortKey="Mccray, Paul B Jr" sort="Mccray, Paul B Jr" uniqKey="Mccray P" first="Paul B. Jr" last="Mccray">Paul B. Jr Mccray</name>
<affiliation><inist:fA14 i1="01"><s1>Department of Pediatrics, University of Iowa</s1>
<s2>Iowa City, Iowa 52242</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>14 aut.</sZ>
</inist:fA14>
</affiliation>
<affiliation><inist:fA14 i1="02"><s1>Department of Genetics, University of Iowa</s1>
<s2>Iowa City, Iowa 52242</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Pewe, Lecia" sort="Pewe, Lecia" uniqKey="Pewe L" first="Lecia" last="Pewe">Lecia Pewe</name>
<affiliation><inist:fA14 i1="03"><s1>Department of Microbiology, University of Iowa</s1>
<s2>Iowa City, Iowa 52242</s2>
<s3>USA</s3>
<sZ>2 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>14 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Wohlford Lenane, Christine" sort="Wohlford Lenane, Christine" uniqKey="Wohlford Lenane C" first="Christine" last="Wohlford-Lenane">Christine Wohlford-Lenane</name>
<affiliation><inist:fA14 i1="01"><s1>Department of Pediatrics, University of Iowa</s1>
<s2>Iowa City, Iowa 52242</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>14 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Hickey, Melissa" sort="Hickey, Melissa" uniqKey="Hickey M" first="Melissa" last="Hickey">Melissa Hickey</name>
<affiliation><inist:fA14 i1="01"><s1>Department of Pediatrics, University of Iowa</s1>
<s2>Iowa City, Iowa 52242</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>14 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Manzel, Lori" sort="Manzel, Lori" uniqKey="Manzel L" first="Lori" last="Manzel">Lori Manzel</name>
<affiliation><inist:fA14 i1="04"><s1>Department of Internal Medicine, University of Iowa</s1>
<s2>Iowa City, Iowa 52242</s2>
<s3>USA</s3>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>13 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Lei Shi" sort="Lei Shi" uniqKey="Lei Shi" last="Lei Shi">LEI SHI</name>
<affiliation><inist:fA14 i1="04"><s1>Department of Internal Medicine, University of Iowa</s1>
<s2>Iowa City, Iowa 52242</s2>
<s3>USA</s3>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>13 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Netland, Jason" sort="Netland, Jason" uniqKey="Netland J" first="Jason" last="Netland">Jason Netland</name>
<affiliation><inist:fA14 i1="05"><s1>Department of Interdisciplinary Programs in Immunology, University of Iowa</s1>
<s2>Iowa City, Iowa 52242</s2>
<s3>USA</s3>
<sZ>7 aut.</sZ>
<sZ>14 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Hong Peng Jia" sort="Hong Peng Jia" uniqKey="Hong Peng Jia" last="Hong Peng Jia">HONG PENG JIA</name>
<affiliation><inist:fA14 i1="01"><s1>Department of Pediatrics, University of Iowa</s1>
<s2>Iowa City, Iowa 52242</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>14 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Halabi, Carmen" sort="Halabi, Carmen" uniqKey="Halabi C" first="Carmen" last="Halabi">Carmen Halabi</name>
<affiliation><inist:fA14 i1="02"><s1>Department of Genetics, University of Iowa</s1>
<s2>Iowa City, Iowa 52242</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
</affiliation>
<affiliation><inist:fA14 i1="03"><s1>Department of Microbiology, University of Iowa</s1>
<s2>Iowa City, Iowa 52242</s2>
<s3>USA</s3>
<sZ>2 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>14 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Sigmund, Curt D" sort="Sigmund, Curt D" uniqKey="Sigmund C" first="Curt D." last="Sigmund">Curt D. Sigmund</name>
<affiliation><inist:fA14 i1="02"><s1>Department of Genetics, University of Iowa</s1>
<s2>Iowa City, Iowa 52242</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
</affiliation>
<affiliation><inist:fA14 i1="03"><s1>Department of Microbiology, University of Iowa</s1>
<s2>Iowa City, Iowa 52242</s2>
<s3>USA</s3>
<sZ>2 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>14 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Meyerholz, David K" sort="Meyerholz, David K" uniqKey="Meyerholz D" first="David K." last="Meyerholz">David K. Meyerholz</name>
<affiliation><inist:fA14 i1="06"><s1>Department of Pathology, University of Iowa</s1>
<s2>Iowa City, Iowa 52242</s2>
<s3>USA</s3>
<sZ>11 aut.</sZ>
<sZ>12 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Kirby, Patricia" sort="Kirby, Patricia" uniqKey="Kirby P" first="Patricia" last="Kirby">Patricia Kirby</name>
<affiliation><inist:fA14 i1="06"><s1>Department of Pathology, University of Iowa</s1>
<s2>Iowa City, Iowa 52242</s2>
<s3>USA</s3>
<sZ>11 aut.</sZ>
<sZ>12 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Look, Dwight C" sort="Look, Dwight C" uniqKey="Look D" first="Dwight C." last="Look">Dwight C. Look</name>
<affiliation><inist:fA14 i1="04"><s1>Department of Internal Medicine, University of Iowa</s1>
<s2>Iowa City, Iowa 52242</s2>
<s3>USA</s3>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>13 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Perlman, Stanley" sort="Perlman, Stanley" uniqKey="Perlman S" first="Stanley" last="Perlman">Stanley Perlman</name>
<affiliation><inist:fA14 i1="01"><s1>Department of Pediatrics, University of Iowa</s1>
<s2>Iowa City, Iowa 52242</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>14 aut.</sZ>
</inist:fA14>
</affiliation>
<affiliation><inist:fA14 i1="03"><s1>Department of Microbiology, University of Iowa</s1>
<s2>Iowa City, Iowa 52242</s2>
<s3>USA</s3>
<sZ>2 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>14 aut.</sZ>
</inist:fA14>
</affiliation>
<affiliation><inist:fA14 i1="05"><s1>Department of Interdisciplinary Programs in Immunology, University of Iowa</s1>
<s2>Iowa City, Iowa 52242</s2>
<s3>USA</s3>
<sZ>7 aut.</sZ>
<sZ>14 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Journal of virology</title>
<title level="j" type="abbreviated">J. virol.</title>
<idno type="ISSN">0022-538X</idno>
<imprint><date when="2007">2007</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Journal of virology</title>
<title level="j" type="abbreviated">J. virol.</title>
<idno type="ISSN">0022-538X</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Coronavirus</term>
<term>Infection</term>
<term>Mouse</term>
<term>Severe acute respiratory syndrome</term>
<term>Virology</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Souris</term>
<term>Coronavirus</term>
<term>Virologie</term>
<term>Infection</term>
<term>Syndrome respiratoire aigu sévère</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The severe acute respiratory syndrome (SARS), caused by a novel coronavirus (SARS-CoV), resulted in substantial morbidity, mortality, and economic losses during the 2003 epidemic. While SARS-CoV infection has not recurred to a significant extent since 2003, it still remains a potential threat. Understanding of SARS and development of therapeutic approaches have been hampered by the absence of an animal model that mimics the human disease and is reproducible. Here we show that transgenic mice that express the SARS-CoV receptor (human angiotensin-converting enzyme 2 [hACE2]) in airway and other epithelia develop a rapidly lethal infection after intranasal inoculation with a human strain of the virus. Infection begins in airway epithelia, with subsequent alveolar involvement and extrapulmonary virus spread to the brain. Infection results in macrophage and lymphocyte infiltration in the lungs and upregulation of proinflammatory cytokines and chemokines in both the lung and the brain. This model of lethal infection with SARS-CoV should be useful for studies of pathogenesis and for the development of antiviral therapies.</div>
</front>
</TEI>
<inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0022-538X</s0>
</fA01>
<fA03 i2="1"><s0>J. virol.</s0>
</fA03>
<fA05><s2>81</s2>
</fA05>
<fA06><s2>2</s2>
</fA06>
<fA08 i1="01" i2="1" l="ENG"><s1>Lethal infection of K18-hACE2 mice infected with severe acute respiratory syndrome coronavirus</s1>
</fA08>
<fA11 i1="01" i2="1"><s1>MCCRAY (Paul B. JR)</s1>
</fA11>
<fA11 i1="02" i2="1"><s1>PEWE (Lecia)</s1>
</fA11>
<fA11 i1="03" i2="1"><s1>WOHLFORD-LENANE (Christine)</s1>
</fA11>
<fA11 i1="04" i2="1"><s1>HICKEY (Melissa)</s1>
</fA11>
<fA11 i1="05" i2="1"><s1>MANZEL (Lori)</s1>
</fA11>
<fA11 i1="06" i2="1"><s1>LEI SHI</s1>
</fA11>
<fA11 i1="07" i2="1"><s1>NETLAND (Jason)</s1>
</fA11>
<fA11 i1="08" i2="1"><s1>HONG PENG JIA</s1>
</fA11>
<fA11 i1="09" i2="1"><s1>HALABI (Carmen)</s1>
</fA11>
<fA11 i1="10" i2="1"><s1>SIGMUND (Curt D.)</s1>
</fA11>
<fA11 i1="11" i2="1"><s1>MEYERHOLZ (David K.)</s1>
</fA11>
<fA11 i1="12" i2="1"><s1>KIRBY (Patricia)</s1>
</fA11>
<fA11 i1="13" i2="1"><s1>LOOK (Dwight C.)</s1>
</fA11>
<fA11 i1="14" i2="1"><s1>PERLMAN (Stanley)</s1>
</fA11>
<fA14 i1="01"><s1>Department of Pediatrics, University of Iowa</s1>
<s2>Iowa City, Iowa 52242</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>8 aut.</sZ>
<sZ>14 aut.</sZ>
</fA14>
<fA14 i1="02"><s1>Department of Genetics, University of Iowa</s1>
<s2>Iowa City, Iowa 52242</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
</fA14>
<fA14 i1="03"><s1>Department of Microbiology, University of Iowa</s1>
<s2>Iowa City, Iowa 52242</s2>
<s3>USA</s3>
<sZ>2 aut.</sZ>
<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>14 aut.</sZ>
</fA14>
<fA14 i1="04"><s1>Department of Internal Medicine, University of Iowa</s1>
<s2>Iowa City, Iowa 52242</s2>
<s3>USA</s3>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>13 aut.</sZ>
</fA14>
<fA14 i1="05"><s1>Department of Interdisciplinary Programs in Immunology, University of Iowa</s1>
<s2>Iowa City, Iowa 52242</s2>
<s3>USA</s3>
<sZ>7 aut.</sZ>
<sZ>14 aut.</sZ>
</fA14>
<fA14 i1="06"><s1>Department of Pathology, University of Iowa</s1>
<s2>Iowa City, Iowa 52242</s2>
<s3>USA</s3>
<sZ>11 aut.</sZ>
<sZ>12 aut.</sZ>
</fA14>
<fA20><s1>813-821</s1>
</fA20>
<fA21><s1>2007</s1>
</fA21>
<fA23 i1="01"><s0>ENG</s0>
</fA23>
<fA43 i1="01"><s1>INIST</s1>
<s2>13592</s2>
<s5>354000159387240380</s5>
</fA43>
<fA44><s0>0000</s0>
<s1>© 2007 INIST-CNRS. All rights reserved.</s1>
</fA44>
<fA45><s0>43 ref.</s0>
</fA45>
<fA47 i1="01" i2="1"><s0>07-0182099</s0>
</fA47>
<fA60><s1>P</s1>
</fA60>
<fA61><s0>A</s0>
</fA61>
<fA64 i1="01" i2="1"><s0>Journal of virology</s0>
</fA64>
<fA66 i1="01"><s0>USA</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>The severe acute respiratory syndrome (SARS), caused by a novel coronavirus (SARS-CoV), resulted in substantial morbidity, mortality, and economic losses during the 2003 epidemic. While SARS-CoV infection has not recurred to a significant extent since 2003, it still remains a potential threat. Understanding of SARS and development of therapeutic approaches have been hampered by the absence of an animal model that mimics the human disease and is reproducible. Here we show that transgenic mice that express the SARS-CoV receptor (human angiotensin-converting enzyme 2 [hACE2]) in airway and other epithelia develop a rapidly lethal infection after intranasal inoculation with a human strain of the virus. Infection begins in airway epithelia, with subsequent alveolar involvement and extrapulmonary virus spread to the brain. Infection results in macrophage and lymphocyte infiltration in the lungs and upregulation of proinflammatory cytokines and chemokines in both the lung and the brain. This model of lethal infection with SARS-CoV should be useful for studies of pathogenesis and for the development of antiviral therapies.</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>002A05C10</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Souris</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>Mouse</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Ratón</s0>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>02</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Virologie</s0>
<s5>05</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Virology</s0>
<s5>05</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Virología</s0>
<s5>05</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Infection</s0>
<s5>14</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Infection</s0>
<s5>14</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Infección</s0>
<s5>14</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Syndrome respiratoire aigu sévère</s0>
<s2>NM</s2>
<s5>15</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Severe acute respiratory syndrome</s0>
<s2>NM</s2>
<s5>15</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Síndrome respiratorio agudo severo</s0>
<s2>NM</s2>
<s5>15</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Rodentia</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Rodentia</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Rodentia</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Mammalia</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Mammalia</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Mammalia</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Vertebrata</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Vertebrata</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Vertebrata</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="06" i2="X" l="ENG"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="06" i2="X" l="SPA"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="07" i2="X" l="FRE"><s0>Appareil respiratoire pathologie</s0>
<s5>13</s5>
</fC07>
<fC07 i1="07" i2="X" l="ENG"><s0>Respiratory disease</s0>
<s5>13</s5>
</fC07>
<fC07 i1="07" i2="X" l="SPA"><s0>Aparato respiratorio patología</s0>
<s5>13</s5>
</fC07>
<fC07 i1="08" i2="X" l="FRE"><s0>Virose</s0>
</fC07>
<fC07 i1="08" i2="X" l="ENG"><s0>Viral disease</s0>
</fC07>
<fC07 i1="08" i2="X" l="SPA"><s0>Virosis</s0>
</fC07>
<fC07 i1="09" i2="X" l="FRE"><s0>Poumon pathologie</s0>
<s5>16</s5>
</fC07>
<fC07 i1="09" i2="X" l="ENG"><s0>Lung disease</s0>
<s5>16</s5>
</fC07>
<fC07 i1="09" i2="X" l="SPA"><s0>Pulmón patología</s0>
<s5>16</s5>
</fC07>
<fN21><s1>122</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
</standard>
<server><NO>PASCAL 07-0182099 INIST</NO>
<ET>Lethal infection of K18-hACE2 mice infected with severe acute respiratory syndrome coronavirus</ET>
<AU>MCCRAY (Paul B. JR); PEWE (Lecia); WOHLFORD-LENANE (Christine); HICKEY (Melissa); MANZEL (Lori); LEI SHI; NETLAND (Jason); HONG PENG JIA; HALABI (Carmen); SIGMUND (Curt D.); MEYERHOLZ (David K.); KIRBY (Patricia); LOOK (Dwight C.); PERLMAN (Stanley)</AU>
<AF>Department of Pediatrics, University of Iowa/Iowa City, Iowa 52242/Etats-Unis (1 aut., 3 aut., 4 aut., 8 aut., 14 aut.); Department of Genetics, University of Iowa/Iowa City, Iowa 52242/Etats-Unis (1 aut., 9 aut., 10 aut.); Department of Microbiology, University of Iowa/Iowa City, Iowa 52242/Etats-Unis (2 aut., 9 aut., 10 aut., 14 aut.); Department of Internal Medicine, University of Iowa/Iowa City, Iowa 52242/Etats-Unis (5 aut., 6 aut., 13 aut.); Department of Interdisciplinary Programs in Immunology, University of Iowa/Iowa City, Iowa 52242/Etats-Unis (7 aut., 14 aut.); Department of Pathology, University of Iowa/Iowa City, Iowa 52242/Etats-Unis (11 aut., 12 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Journal of virology; ISSN 0022-538X; Etats-Unis; Da. 2007; Vol. 81; No. 2; Pp. 813-821; Bibl. 43 ref.</SO>
<LA>Anglais</LA>
<EA>The severe acute respiratory syndrome (SARS), caused by a novel coronavirus (SARS-CoV), resulted in substantial morbidity, mortality, and economic losses during the 2003 epidemic. While SARS-CoV infection has not recurred to a significant extent since 2003, it still remains a potential threat. Understanding of SARS and development of therapeutic approaches have been hampered by the absence of an animal model that mimics the human disease and is reproducible. Here we show that transgenic mice that express the SARS-CoV receptor (human angiotensin-converting enzyme 2 [hACE2]) in airway and other epithelia develop a rapidly lethal infection after intranasal inoculation with a human strain of the virus. Infection begins in airway epithelia, with subsequent alveolar involvement and extrapulmonary virus spread to the brain. Infection results in macrophage and lymphocyte infiltration in the lungs and upregulation of proinflammatory cytokines and chemokines in both the lung and the brain. This model of lethal infection with SARS-CoV should be useful for studies of pathogenesis and for the development of antiviral therapies.</EA>
<CC>002A05C10</CC>
<FD>Souris; Coronavirus; Virologie; Infection; Syndrome respiratoire aigu sévère</FD>
<FG>Rodentia; Mammalia; Vertebrata; Coronaviridae; Nidovirales; Virus; Appareil respiratoire pathologie; Virose; Poumon pathologie</FG>
<ED>Mouse; Coronavirus; Virology; Infection; Severe acute respiratory syndrome</ED>
<EG>Rodentia; Mammalia; Vertebrata; Coronaviridae; Nidovirales; Virus; Respiratory disease; Viral disease; Lung disease</EG>
<SD>Ratón; Coronavirus; Virología; Infección; Síndrome respiratorio agudo severo</SD>
<LO>INIST-13592.354000159387240380</LO>
<ID>07-0182099</ID>
</server>
</inist>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/PascalFrancis/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000384 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PascalFrancis/Corpus/biblio.hfd -nk 000384 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= SrasV1 |flux= PascalFrancis |étape= Corpus |type= RBID |clé= Pascal:07-0182099 |texte= Lethal infection of K18-hACE2 mice infected with severe acute respiratory syndrome coronavirus }}
This area was generated with Dilib version V0.6.33. |