SrasV1, PascalFrancis, Corpus, bibRecord, 000310

In vitro inhibition of coronavirus replications by the traditionally used medicinal herbal extracts, Cimicifuga rhizoma, Meliae cortex, Coptidis rhizoma, and Phellodendron cortex

Identifieur interne : 000310 ( PascalFrancis/Corpus ); précédent : 000309; suivant : 000311

In vitro inhibition of coronavirus replications by the traditionally used medicinal herbal extracts, Cimicifuga rhizoma, Meliae cortex, Coptidis rhizoma, and Phellodendron cortex

Auteurs : Hye-Young Kim ; Hyun-Soo Shin ; Hyun Park ; Youn-Chul Kim ; YONG GAB YUN ; Sun Park ; Ho-Joon Shin ; Kyongmin Kim

Source :

Journal of clinical virology [ 1386-6532 ] ; 2008.

RBID : Pascal:08-0139729

Descripteurs français

Pascal (Inist)
- Coronavirus, Replication in vitro, Phellodendron, Réplication, Antiviral, Microbiologie, Virologie.

English descriptors

KwdEn :
- Antiviral, Coronavirus, In vitro replication, Microbiology, Phellodendron, Replication, Virology.

Abstract

Background: A search for new anti-coronaviral drugs to treat coronaviral infections was motivated by an outbreak of severe acute respiratory syndrome (SARS). Objectives: In order to find drugs that treat coronavirus infections, including SARS, we screened traditional medicinal herbal extracts and evaluated their antiviral activities on coronavirus replication. Study design: We employed a plaque assay to evaluate the effect of 22 medicinal herbal extracts on virus replication. We determined the 50% effective concentration (EC₅₀) of each extract that was necessary to inhibit the replication of mouse hepatitis virus A59 (MHV-A59); we also determined 50% cytotoxic concentrations (CC₅₀) for each extract. Northern and Western blot analyzes were performed to investigate antiviral activity in MHV-infected DBT cells, including virus entry, viral RNA and protein expression, and virus release. Coronavirus specific inhibition was also demonstrated using porcine epidemic diarrhea virus (PEDV). Results: Cimicifuga rhizoma, Meliae cortex, Coptidis rhizoma, Phellodendron cortex and Sophora subprostrata radix decreased the MHV production and the intracellular viral RNA and protein expression with EC₅₀ values ranging from 2.0 to 27.5 μg/ml. These extracts also significantly decreased PEDV production and less dramatically decreased vesicular stomatitis virus (VSV) production in vitro. Conclusions: The extracts selected strongly inhibited MHV replication and could be potential candidates for new anti-coronavirus drugs.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

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A03		`1`		`@0 J. clin. virol.`
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A08	`01`	`1`	`ENG`	`@1 In vitro inhibition of coronavirus replications by the traditionally used medicinal herbal extracts, Cimicifuga rhizoma, Meliae cortex, Coptidis rhizoma, and Phellodendron cortex`
A11	`01`	`1`		`@1 KIM (Hye-Young)`
A11	`02`	`1`		`@1 SHIN (Hyun-Soo)`
A11	`03`	`1`		`@1 PARK (Hyun)`
A11	`04`	`1`		`@1 KIM (Youn-Chul)`
A11	`05`	`1`		`@1 YONG GAB YUN`
A11	`06`	`1`		`@1 PARK (Sun)`
A11	`07`	`1`		`@1 SHIN (Ho-Joon)`
A11	`08`	`1`		`@1 KIM (Kyongmin)`
A14	`01`			`@1 Department of Microbiology, Ajou University School of Medicine @2 Suwon @3 KOR @Z 1 aut. @Z 2 aut. @Z 6 aut. @Z 7 aut. @Z 8 aut.`
A14	`02`			`@1 Zoonosis Research Center, Wonkwang University @2 Iksan, Chonbuk @3 KOR @Z 3 aut. @Z 4 aut. @Z 5 aut. @Z 8 aut.`
A14	`03`			`@1 Department of Infection Biology, School of Medicine, Wonkwang University @2 Iksan, Chonbuk @3 KOR @Z 3 aut.`
A14	`04`			`@1 College of Pharmacy, Wonkwang University @2 Iksan, Chonbuk @3 KOR @Z 4 aut.`
A14	`05`			`@1 Department of Oriental Medicine, Wonkwang University @2 Iksan, Chonbuk @3 KOR @Z 5 aut.`
A20				`@1 122-128`
A21				`@1 2008`
A23	`01`			`@0 ENG`
A43	`01`			`@1 INIST @2 26272 @5 354000175006880120`
A44				`@0 0000 @1 © 2008 INIST-CNRS. All rights reserved.`
A45				`@0 3/4 p.`
A47	`01`	`1`		`@0 08-0139729`
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A61				`@0 A`
A64	`01`	`1`		`@0 Journal of clinical virology`
A66	`01`			`@0 NLD`
C01	`01`		`ENG`	@0 Background: A search for new anti-coronaviral drugs to treat coronaviral infections was motivated by an outbreak of severe acute respiratory syndrome (SARS). Objectives: In order to find drugs that treat coronavirus infections, including SARS, we screened traditional medicinal herbal extracts and evaluated their antiviral activities on coronavirus replication. Study design: We employed a plaque assay to evaluate the effect of 22 medicinal herbal extracts on virus replication. We determined the 50% effective concentration (EC₅₀) of each extract that was necessary to inhibit the replication of mouse hepatitis virus A59 (MHV-A59); we also determined 50% cytotoxic concentrations (CC₅₀) for each extract. Northern and Western blot analyzes were performed to investigate antiviral activity in MHV-infected DBT cells, including virus entry, viral RNA and protein expression, and virus release. Coronavirus specific inhibition was also demonstrated using porcine epidemic diarrhea virus (PEDV). Results: Cimicifuga rhizoma, Meliae cortex, Coptidis rhizoma, Phellodendron cortex and Sophora subprostrata radix decreased the MHV production and the intracellular viral RNA and protein expression with EC₅₀ values ranging from 2.0 to 27.5 μg/ml. These extracts also significantly decreased PEDV production and less dramatically decreased vesicular stomatitis virus (VSV) production in vitro. Conclusions: The extracts selected strongly inhibited MHV replication and could be potential candidates for new anti-coronavirus drugs.
C02	`01`	`X`		`@0 002A05C10`
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C03	`02`	`X`	`FRE`	`@0 Replication in vitro @5 05`
C03	`02`	`X`	`ENG`	`@0 In vitro replication @5 05`
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C03	`07`	`X`	`FRE`	`@0 Virologie @5 10`
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C07	`05`	`X`	`FRE`	`@0 Dicotyledones @2 NS`
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C07	`07`	`X`	`FRE`	`@0 Spermatophyta @2 NS`
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N21				`@1 084`
N44	`01`			`@1 OTO`
N82				`@1 OTO`

Format Inist (serveur)

NO :	PASCAL 08-0139729 INIST
ET :	In vitro inhibition of coronavirus replications by the traditionally used medicinal herbal extracts, Cimicifuga rhizoma, Meliae cortex, Coptidis rhizoma, and Phellodendron cortex
AU :	KIM (Hye-Young); SHIN (Hyun-Soo); PARK (Hyun); KIM (Youn-Chul); YONG GAB YUN; PARK (Sun); SHIN (Ho-Joon); KIM (Kyongmin)
AF :	Department of Microbiology, Ajou University School of Medicine/Suwon/Corée, République de (1 aut., 2 aut., 6 aut., 7 aut., 8 aut.); Zoonosis Research Center, Wonkwang University/Iksan, Chonbuk/Corée, République de (3 aut., 4 aut., 5 aut., 8 aut.); Department of Infection Biology, School of Medicine, Wonkwang University/Iksan, Chonbuk/Corée, République de (3 aut.); College of Pharmacy, Wonkwang University/Iksan, Chonbuk/Corée, République de (4 aut.); Department of Oriental Medicine, Wonkwang University/Iksan, Chonbuk/Corée, République de (5 aut.)
DT :	Publication en série; Niveau analytique
SO :	Journal of clinical virology; ISSN 1386-6532; Pays-Bas; Da. 2008; Vol. 41; No. 2; Pp. 122-128; Bibl. 3/4 p.
LA :	Anglais
EA :	Background: A search for new anti-coronaviral drugs to treat coronaviral infections was motivated by an outbreak of severe acute respiratory syndrome (SARS). Objectives: In order to find drugs that treat coronavirus infections, including SARS, we screened traditional medicinal herbal extracts and evaluated their antiviral activities on coronavirus replication. Study design: We employed a plaque assay to evaluate the effect of 22 medicinal herbal extracts on virus replication. We determined the 50% effective concentration (EC₅₀) of each extract that was necessary to inhibit the replication of mouse hepatitis virus A59 (MHV-A59); we also determined 50% cytotoxic concentrations (CC₅₀) for each extract. Northern and Western blot analyzes were performed to investigate antiviral activity in MHV-infected DBT cells, including virus entry, viral RNA and protein expression, and virus release. Coronavirus specific inhibition was also demonstrated using porcine epidemic diarrhea virus (PEDV). Results: Cimicifuga rhizoma, Meliae cortex, Coptidis rhizoma, Phellodendron cortex and Sophora subprostrata radix decreased the MHV production and the intracellular viral RNA and protein expression with EC₅₀ values ranging from 2.0 to 27.5 μg/ml. These extracts also significantly decreased PEDV production and less dramatically decreased vesicular stomatitis virus (VSV) production in vitro. Conclusions: The extracts selected strongly inhibited MHV replication and could be potential candidates for new anti-coronavirus drugs.
CC :	002A05C10; 002B05C02J
FD :	Coronavirus; Replication in vitro; Phellodendron; Réplication; Antiviral; Microbiologie; Virologie
FG :	Coronaviridae; Nidovirales; Virus; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta
ED :	Coronavirus; In vitro replication; Phellodendron; Replication; Antiviral; Microbiology; Virology
EG :	Coronaviridae; Nidovirales; Virus; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta
SD :	Coronavirus; Replicación in vitro; Phellodendron; Replicación; Antiviral; Microbiología; Virología
LO :	INIST-26272.354000175006880120
ID :	08-0139729

Links to Exploration step

Pascal:08-0139729

Le document en format XML

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<author><name sortKey="Park, Sun" sort="Park, Sun" uniqKey="Park S" first="Sun" last="Park">Sun Park</name>
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<author><name sortKey="Kim, Kyongmin" sort="Kim, Kyongmin" uniqKey="Kim K" first="Kyongmin" last="Kim">Kyongmin Kim</name>
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<series><title level="j" type="main">Journal of clinical virology</title>
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<seriesStmt><title level="j" type="main">Journal of clinical virology</title>
<title level="j" type="abbreviated">J. clin. virol.</title>
<idno type="ISSN">1386-6532</idno>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antiviral</term>
<term>Coronavirus</term>
<term>In vitro replication</term>
<term>Microbiology</term>
<term>Phellodendron</term>
<term>Replication</term>
<term>Virology</term>
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<keywords scheme="Pascal" xml:lang="fr"><term>Coronavirus</term>
<term>Replication in vitro</term>
<term>Phellodendron</term>
<term>Réplication</term>
<term>Antiviral</term>
<term>Microbiologie</term>
<term>Virologie</term>
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<front><div type="abstract" xml:lang="en">Background: A search for new anti-coronaviral drugs to treat coronaviral infections was motivated by an outbreak of severe acute respiratory syndrome (SARS). Objectives: In order to find drugs that treat coronavirus infections, including SARS, we screened traditional medicinal herbal extracts and evaluated their antiviral activities on coronavirus replication. Study design: We employed a plaque assay to evaluate the effect of 22 medicinal herbal extracts on virus replication. We determined the 50% effective concentration (EC<sub>50</sub>
) of each extract that was necessary to inhibit the replication of mouse hepatitis virus A59 (MHV-A59); we also determined 50% cytotoxic concentrations (CC<sub>50</sub>
) for each extract. Northern and Western blot analyzes were performed to investigate antiviral activity in MHV-infected DBT cells, including virus entry, viral RNA and protein expression, and virus release. Coronavirus specific inhibition was also demonstrated using porcine epidemic diarrhea virus (PEDV). Results: Cimicifuga rhizoma, Meliae cortex, Coptidis rhizoma, Phellodendron cortex and Sophora subprostrata radix decreased the MHV production and the intracellular viral RNA and protein expression with EC<sub>50</sub>
 values ranging from 2.0 to 27.5 μg/ml. These extracts also significantly decreased PEDV production and less dramatically decreased vesicular stomatitis virus (VSV) production in vitro. Conclusions: The extracts selected strongly inhibited MHV replication and could be potential candidates for new anti-coronavirus drugs.</div>
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<fA08 i1="01" i2="1" l="ENG"><s1>In vitro inhibition of coronavirus replications by the traditionally used medicinal herbal extracts, Cimicifuga rhizoma, Meliae cortex, Coptidis rhizoma, and Phellodendron cortex</s1>
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<fA11 i1="01" i2="1"><s1>KIM (Hye-Young)</s1>
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<fA11 i1="02" i2="1"><s1>SHIN (Hyun-Soo)</s1>
</fA11>
<fA11 i1="03" i2="1"><s1>PARK (Hyun)</s1>
</fA11>
<fA11 i1="04" i2="1"><s1>KIM (Youn-Chul)</s1>
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<fA11 i1="05" i2="1"><s1>YONG GAB YUN</s1>
</fA11>
<fA11 i1="06" i2="1"><s1>PARK (Sun)</s1>
</fA11>
<fA11 i1="07" i2="1"><s1>SHIN (Ho-Joon)</s1>
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<fA11 i1="08" i2="1"><s1>KIM (Kyongmin)</s1>
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<fA14 i1="01"><s1>Department of Microbiology, Ajou University School of Medicine</s1>
<s2>Suwon</s2>
<s3>KOR</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
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<fA14 i1="02"><s1>Zoonosis Research Center, Wonkwang University</s1>
<s2>Iksan, Chonbuk</s2>
<s3>KOR</s3>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>8 aut.</sZ>
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<fA14 i1="03"><s1>Department of Infection Biology, School of Medicine, Wonkwang University</s1>
<s2>Iksan, Chonbuk</s2>
<s3>KOR</s3>
<sZ>3 aut.</sZ>
</fA14>
<fA14 i1="04"><s1>College of Pharmacy, Wonkwang University</s1>
<s2>Iksan, Chonbuk</s2>
<s3>KOR</s3>
<sZ>4 aut.</sZ>
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<fA14 i1="05"><s1>Department of Oriental Medicine, Wonkwang University</s1>
<s2>Iksan, Chonbuk</s2>
<s3>KOR</s3>
<sZ>5 aut.</sZ>
</fA14>
<fA20><s1>122-128</s1>
</fA20>
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<fC01 i1="01" l="ENG"><s0>Background: A search for new anti-coronaviral drugs to treat coronaviral infections was motivated by an outbreak of severe acute respiratory syndrome (SARS). Objectives: In order to find drugs that treat coronavirus infections, including SARS, we screened traditional medicinal herbal extracts and evaluated their antiviral activities on coronavirus replication. Study design: We employed a plaque assay to evaluate the effect of 22 medicinal herbal extracts on virus replication. We determined the 50% effective concentration (EC<sub>50</sub>
) of each extract that was necessary to inhibit the replication of mouse hepatitis virus A59 (MHV-A59); we also determined 50% cytotoxic concentrations (CC<sub>50</sub>
) for each extract. Northern and Western blot analyzes were performed to investigate antiviral activity in MHV-infected DBT cells, including virus entry, viral RNA and protein expression, and virus release. Coronavirus specific inhibition was also demonstrated using porcine epidemic diarrhea virus (PEDV). Results: Cimicifuga rhizoma, Meliae cortex, Coptidis rhizoma, Phellodendron cortex and Sophora subprostrata radix decreased the MHV production and the intracellular viral RNA and protein expression with EC<sub>50</sub>
 values ranging from 2.0 to 27.5 μg/ml. These extracts also significantly decreased PEDV production and less dramatically decreased vesicular stomatitis virus (VSV) production in vitro. Conclusions: The extracts selected strongly inhibited MHV replication and could be potential candidates for new anti-coronavirus drugs.</s0>
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<server><NO>PASCAL 08-0139729 INIST</NO>
<ET>In vitro inhibition of coronavirus replications by the traditionally used medicinal herbal extracts, Cimicifuga rhizoma, Meliae cortex, Coptidis rhizoma, and Phellodendron cortex</ET>
<AU>KIM (Hye-Young); SHIN (Hyun-Soo); PARK (Hyun); KIM (Youn-Chul); YONG GAB YUN; PARK (Sun); SHIN (Ho-Joon); KIM (Kyongmin)</AU>
<AF>Department of Microbiology, Ajou University School of Medicine/Suwon/Corée, République de (1 aut., 2 aut., 6 aut., 7 aut., 8 aut.); Zoonosis Research Center, Wonkwang University/Iksan, Chonbuk/Corée, République de (3 aut., 4 aut., 5 aut., 8 aut.); Department of Infection Biology, School of Medicine, Wonkwang University/Iksan, Chonbuk/Corée, République de (3 aut.); College of Pharmacy, Wonkwang University/Iksan, Chonbuk/Corée, République de (4 aut.); Department of Oriental Medicine, Wonkwang University/Iksan, Chonbuk/Corée, République de (5 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Journal of clinical virology; ISSN 1386-6532; Pays-Bas; Da. 2008; Vol. 41; No. 2; Pp. 122-128; Bibl. 3/4 p.</SO>
<LA>Anglais</LA>
<EA>Background: A search for new anti-coronaviral drugs to treat coronaviral infections was motivated by an outbreak of severe acute respiratory syndrome (SARS). Objectives: In order to find drugs that treat coronavirus infections, including SARS, we screened traditional medicinal herbal extracts and evaluated their antiviral activities on coronavirus replication. Study design: We employed a plaque assay to evaluate the effect of 22 medicinal herbal extracts on virus replication. We determined the 50% effective concentration (EC<sub>50</sub>
) of each extract that was necessary to inhibit the replication of mouse hepatitis virus A59 (MHV-A59); we also determined 50% cytotoxic concentrations (CC<sub>50</sub>
) for each extract. Northern and Western blot analyzes were performed to investigate antiviral activity in MHV-infected DBT cells, including virus entry, viral RNA and protein expression, and virus release. Coronavirus specific inhibition was also demonstrated using porcine epidemic diarrhea virus (PEDV). Results: Cimicifuga rhizoma, Meliae cortex, Coptidis rhizoma, Phellodendron cortex and Sophora subprostrata radix decreased the MHV production and the intracellular viral RNA and protein expression with EC<sub>50</sub>
 values ranging from 2.0 to 27.5 μg/ml. These extracts also significantly decreased PEDV production and less dramatically decreased vesicular stomatitis virus (VSV) production in vitro. Conclusions: The extracts selected strongly inhibited MHV replication and could be potential candidates for new anti-coronavirus drugs.</EA>
<CC>002A05C10; 002B05C02J</CC>
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<ED>Coronavirus; In vitro replication; Phellodendron; Replication; Antiviral; Microbiology; Virology</ED>
<EG>Coronaviridae; Nidovirales; Virus; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta</EG>
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In vitro inhibition of coronavirus replications by the traditionally used medicinal herbal extracts, Cimicifuga rhizoma, Meliae cortex, Coptidis rhizoma, and Phellodendron cortex

In vitro inhibition of coronavirus replications by the traditionally used medicinal herbal extracts, Cimicifuga rhizoma, Meliae cortex, Coptidis rhizoma, and Phellodendron cortex

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