RNA aptamer-based sensitive detection of SARS coronavirus nucleocapsid protein
Identifieur interne : 000174 ( PascalFrancis/Corpus ); précédent : 000173; suivant : 000175RNA aptamer-based sensitive detection of SARS coronavirus nucleocapsid protein
Auteurs : Dae-Gyun Ahn ; Il-Ji Jeon ; JUNG DONG KIM ; Min-Sun Song ; Seung-Ryul Han ; Seong-Wook Lee ; Hyungil Jung ; Jong-Won OhSource :
- Analyst : (London. 1877. Print) [ 0003-2654 ] ; 2009.
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- Pascal (Inist)
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- KwdEn :
Abstract
Severe acute respiratory syndrome coronavirus (SARS-CoV) is the etiological agent of a newly emerged disease SARS. The SARS-CoV nucleocapsid (N) protein is one of the most abundant structural proteins and serves as a diagnostic marker for accurate and sensitive detection of the virus. Using a SELEX (systematic evolution of ligand by exponential enrichment) procedure and recombinant N protein, we selected a high-affinity RNA aptamer capable of binding to N protein with a dissociation constant of 1.65 nM. Electrophoretic mobility shift assays and RNA competition experiments showed that the selected aptamer recognized selectively the C-terminal region of N protein with high specificity. Using a chemiluminescence immunosorbent assay and a nanoarray aptamer chip with the selected aptamer as an antigen-capturing agent, we could sensitively detect N protein at a concentration as low as 2 pg/ml. These aptamer-antibody hybrid immunoassays may be useful for rapid, sensitive detection of SARS-CoV N protein.
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Format Inist (serveur)
NO : | PASCAL 09-0485565 INIST |
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ET : | RNA aptamer-based sensitive detection of SARS coronavirus nucleocapsid protein |
AU : | AHN (Dae-Gyun); JEON (Il-Ji); JUNG DONG KIM; SONG (Min-Sun); HAN (Seung-Ryul); LEE (Seong-Wook); JUNG (Hyungil); OH (Jong-Won) |
AF : | Department of Biotechnology, Yonsei University/Seoul 120-749/Corée, République de (1 aut., 2 aut., 3 aut., 7 aut., 8 aut.); Department of Molecular Biology, Dankook University/448-701/Corée, République de (4 aut., 5 aut., 6 aut.) |
DT : | Publication en série; Niveau analytique |
SO : | Analyst : (London. 1877. Print); ISSN 0003-2654; Coden ANALAO; Royaume-Uni; Da. 2009; Vol. 134; No. 9; Pp. 1896-1901; Bibl. 30 ref. |
LA : | Anglais |
EA : | Severe acute respiratory syndrome coronavirus (SARS-CoV) is the etiological agent of a newly emerged disease SARS. The SARS-CoV nucleocapsid (N) protein is one of the most abundant structural proteins and serves as a diagnostic marker for accurate and sensitive detection of the virus. Using a SELEX (systematic evolution of ligand by exponential enrichment) procedure and recombinant N protein, we selected a high-affinity RNA aptamer capable of binding to N protein with a dissociation constant of 1.65 nM. Electrophoretic mobility shift assays and RNA competition experiments showed that the selected aptamer recognized selectively the C-terminal region of N protein with high specificity. Using a chemiluminescence immunosorbent assay and a nanoarray aptamer chip with the selected aptamer as an antigen-capturing agent, we could sensitively detect N protein at a concentration as low as 2 pg/ml. These aptamer-antibody hybrid immunoassays may be useful for rapid, sensitive detection of SARS-CoV N protein. |
CC : | 001C04F; 001C04G |
FD : | Marqueur; Enrichissement chimique; Constante dissociation; Mobilité électrophorétique; Analyse chimique; Chimiluminescence; Composé ultratrace; Méthode immunologique; Protéine; Anticorps |
ED : | Marker; Chemical enrichment; Dissociation constant; Electrophoretic mobility; Chemical analysis; Chemiluminescence; Ultratrace compound; Immunological method; Protein; Antibody |
SD : | Marcador; Enriquecimiento químico; Constante disociación; Movilidad electroforética; Análisis químico; Quimioluminiscencia; Compuesto ultrahuella; Método inmunológico; Proteína; Anticuerpo |
LO : | INIST-1036.354000196204190230 |
ID : | 09-0485565 |
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Pascal:09-0485565Le document en format XML
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<front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome coronavirus (SARS-CoV) is the etiological agent of a newly emerged disease SARS. The SARS-CoV nucleocapsid (N) protein is one of the most abundant structural proteins and serves as a diagnostic marker for accurate and sensitive detection of the virus. Using a SELEX (systematic evolution of ligand by exponential enrichment) procedure and recombinant N protein, we selected a high-affinity RNA aptamer capable of binding to N protein with a dissociation constant of 1.65 nM. Electrophoretic mobility shift assays and RNA competition experiments showed that the selected aptamer recognized selectively the C-terminal region of N protein with high specificity. Using a chemiluminescence immunosorbent assay and a nanoarray aptamer chip with the selected aptamer as an antigen-capturing agent, we could sensitively detect N protein at a concentration as low as 2 pg/ml. These aptamer-antibody hybrid immunoassays may be useful for rapid, sensitive detection of SARS-CoV N protein.</div>
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<server><NO>PASCAL 09-0485565 INIST</NO>
<ET>RNA aptamer-based sensitive detection of SARS coronavirus nucleocapsid protein</ET>
<AU>AHN (Dae-Gyun); JEON (Il-Ji); JUNG DONG KIM; SONG (Min-Sun); HAN (Seung-Ryul); LEE (Seong-Wook); JUNG (Hyungil); OH (Jong-Won)</AU>
<AF>Department of Biotechnology, Yonsei University/Seoul 120-749/Corée, République de (1 aut., 2 aut., 3 aut., 7 aut., 8 aut.); Department of Molecular Biology, Dankook University/448-701/Corée, République de (4 aut., 5 aut., 6 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Analyst : (London. 1877. Print); ISSN 0003-2654; Coden ANALAO; Royaume-Uni; Da. 2009; Vol. 134; No. 9; Pp. 1896-1901; Bibl. 30 ref.</SO>
<LA>Anglais</LA>
<EA>Severe acute respiratory syndrome coronavirus (SARS-CoV) is the etiological agent of a newly emerged disease SARS. The SARS-CoV nucleocapsid (N) protein is one of the most abundant structural proteins and serves as a diagnostic marker for accurate and sensitive detection of the virus. Using a SELEX (systematic evolution of ligand by exponential enrichment) procedure and recombinant N protein, we selected a high-affinity RNA aptamer capable of binding to N protein with a dissociation constant of 1.65 nM. Electrophoretic mobility shift assays and RNA competition experiments showed that the selected aptamer recognized selectively the C-terminal region of N protein with high specificity. Using a chemiluminescence immunosorbent assay and a nanoarray aptamer chip with the selected aptamer as an antigen-capturing agent, we could sensitively detect N protein at a concentration as low as 2 pg/ml. These aptamer-antibody hybrid immunoassays may be useful for rapid, sensitive detection of SARS-CoV N protein.</EA>
<CC>001C04F; 001C04G</CC>
<FD>Marqueur; Enrichissement chimique; Constante dissociation; Mobilité électrophorétique; Analyse chimique; Chimiluminescence; Composé ultratrace; Méthode immunologique; Protéine; Anticorps</FD>
<ED>Marker; Chemical enrichment; Dissociation constant; Electrophoretic mobility; Chemical analysis; Chemiluminescence; Ultratrace compound; Immunological method; Protein; Antibody</ED>
<SD>Marcador; Enriquecimiento químico; Constante disociación; Movilidad electroforética; Análisis químico; Quimioluminiscencia; Compuesto ultrahuella; Método inmunológico; Proteína; Anticuerpo</SD>
<LO>INIST-1036.354000196204190230</LO>
<ID>09-0485565</ID>
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