SrasV1, PascalFrancis, Corpus, bibRecord, 000118

SARS coronavirus 8b reduces viral replication by down-regulating E via an ubiquitin-independent proteasome pathway

Identifieur interne : 000118 ( PascalFrancis/Corpus ); précédent : 000117; suivant : 000119

SARS coronavirus 8b reduces viral replication by down-regulating E via an ubiquitin-independent proteasome pathway

Auteurs : Choong-Tat Keng ; Sara Akerström ; Cynthia Sau-Wai Leung ; Leo L. M. Poon ; J. S. Malik Peiris ; Ali Mirazimi ; Yee-Joo Tan

Source :

Microbes and infection [ 1286-4579 ] ; 2011.

RBID : Pascal:11-0108132

Descripteurs français

Pascal (Inist)
- Virus syndrome respiratoire aigu sévère, Réplication, Ubiquitine, Multicatalytic endopeptidase complex, Protéine, Syndrome respiratoire aigu sévère.

English descriptors

KwdEn :
- Multicatalytic endopeptidase complex, Protein, Replication, Severe acute respiratory syndrome, Severe acute respiratory syndrome virus, Ubiquitin.

Abstract

The severe acute respiratory syndrome coronavirus (SARS-CoV) 8b protein, which is not expressed by other known coronaviruses, can down-regulate the envelope (E) protein via a proteasome-dependent pathway. Here, we showed that the down-regulation of E is not dependent on the lysine residues on 8b and the reduction of polyubiquitination of E mutants is not correlated with their down-regulation by 8b, suggesting an ubiquitin-independent proteasome pathway is involved. A time-course study revealed that 8b was expressed at late-stages of SARS-CoV infection. By using Vero E6 cells stably expressing green fluorescence protein-tagged 8b, ectopic expression of 8b was shown to significantly reduce the production of progeny virus and down-regulate E expression. Taken together, these results suggest that 8b negatively modulates virus replication by down-regulating E via an ubiquitin-independent proteasome pathway.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

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A08	`01`	`1`	`ENG`	`@1 SARS coronavirus 8b reduces viral replication by down-regulating E via an ubiquitin-independent proteasome pathway`
A11	`01`	`1`		`@1 KENG (Choong-Tat)`
A11	`02`	`1`		`@1 AKERSTRÖM (Sara)`
A11	`03`	`1`		`@1 LEUNG (Cynthia Sau-Wai)`
A11	`04`	`1`		`@1 POON (Leo L. M.)`
A11	`05`	`1`		`@1 PEIRIS (J. S. Malik)`
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A14	`02`			`@1 Center for Microbiological Preparedness, Swedish Institute for Infectious Disease Control @2 171 82 Solna @3 SWE @Z 2 aut. @Z 6 aut.`
A14	`03`			`@1 Department of Microbiology, Tumor and Cell Biology, Karolinska Institute @2 Stockholm @3 SWE @Z 2 aut. @Z 6 aut.`
A14	`04`			`@1 Department of Microbiology, The University of Hong Kong @3 HKG @Z 3 aut. @Z 4 aut. @Z 5 aut.`
A14	`05`			`@1 HKU-Pasteur Research Centre @3 HKG @Z 5 aut.`
A14	`06`			`@1 Department of Microbiology, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore @3 SGP @Z 7 aut.`
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A21				`@1 2011`
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A44				`@0 0000 @1 © 2011 INIST-CNRS. All rights reserved.`
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C01	`01`		`ENG`	@0 The severe acute respiratory syndrome coronavirus (SARS-CoV) 8b protein, which is not expressed by other known coronaviruses, can down-regulate the envelope (E) protein via a proteasome-dependent pathway. Here, we showed that the down-regulation of E is not dependent on the lysine residues on 8b and the reduction of polyubiquitination of E mutants is not correlated with their down-regulation by 8b, suggesting an ubiquitin-independent proteasome pathway is involved. A time-course study revealed that 8b was expressed at late-stages of SARS-CoV infection. By using Vero E6 cells stably expressing green fluorescence protein-tagged 8b, ectopic expression of 8b was shown to significantly reduce the production of progeny virus and down-regulate E expression. Taken together, these results suggest that 8b negatively modulates virus replication by down-regulating E via an ubiquitin-independent proteasome pathway.
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C03	`02`	`X`	`FRE`	`@0 Réplication @5 05`
C03	`02`	`X`	`ENG`	`@0 Replication @5 05`
C03	`02`	`X`	`SPA`	`@0 Replicación @5 05`
C03	`03`	`X`	`FRE`	`@0 Ubiquitine @5 06`
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C07	`02`	`X`	`ENG`	`@0 Coronaviridae @2 NW`
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C07	`03`	`X`	`FRE`	`@0 Nidovirales @2 NW`
C07	`03`	`X`	`ENG`	`@0 Nidovirales @2 NW`
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C07	`05`	`X`	`FRE`	`@0 Peptidases @2 FE`
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C07	`08`	`X`	`FRE`	`@0 Pathologie de l'appareil respiratoire @5 13`
C07	`08`	`X`	`ENG`	`@0 Respiratory disease @5 13`
C07	`08`	`X`	`SPA`	`@0 Aparato respiratorio patología @5 13`
C07	`09`	`X`	`FRE`	`@0 Virose`
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N44	`01`			`@1 OTO`
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Format Inist (serveur)

NO :	PASCAL 11-0108132 INIST
ET :	SARS coronavirus 8b reduces viral replication by down-regulating E via an ubiquitin-independent proteasome pathway
AU :	KENG (Choong-Tat); AKERSTRÖM (Sara); LEUNG (Cynthia Sau-Wai); POON (Leo L. M.); PEIRIS (J. S. Malik); MIRAZIMI (Ali); TAN (Yee-Joo)
AF :	Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology/Singapour (1 aut., 7 aut.); Center for Microbiological Preparedness, Swedish Institute for Infectious Disease Control/171 82 Solna/Suède (2 aut., 6 aut.); Department of Microbiology, Tumor and Cell Biology, Karolinska Institute/Stockholm/Suède (2 aut., 6 aut.); Department of Microbiology, The University of Hong Kong/Hong-Kong (3 aut., 4 aut., 5 aut.); HKU-Pasteur Research Centre/Hong-Kong (5 aut.); Department of Microbiology, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore/Singapour (7 aut.)
DT :	Publication en série; Niveau analytique
SO :	Microbes and infection; ISSN 1286-4579; Royaume-Uni; Da. 2011; Vol. 13; No. 2; Pp. 179-188; Bibl. 44 ref.
LA :	Anglais
EA :	The severe acute respiratory syndrome coronavirus (SARS-CoV) 8b protein, which is not expressed by other known coronaviruses, can down-regulate the envelope (E) protein via a proteasome-dependent pathway. Here, we showed that the down-regulation of E is not dependent on the lysine residues on 8b and the reduction of polyubiquitination of E mutants is not correlated with their down-regulation by 8b, suggesting an ubiquitin-independent proteasome pathway is involved. A time-course study revealed that 8b was expressed at late-stages of SARS-CoV infection. By using Vero E6 cells stably expressing green fluorescence protein-tagged 8b, ectopic expression of 8b was shown to significantly reduce the production of progeny virus and down-regulate E expression. Taken together, these results suggest that 8b negatively modulates virus replication by down-regulating E via an ubiquitin-independent proteasome pathway.
CC :	002A05
FD :	Virus syndrome respiratoire aigu sévère; Réplication; Ubiquitine; Multicatalytic endopeptidase complex; Protéine; Syndrome respiratoire aigu sévère
FG :	Coronavirus; Coronaviridae; Nidovirales; Virus; Peptidases; Hydrolases; Enzyme; Pathologie de l'appareil respiratoire; Virose; Infection; Pathologie des poumons
ED :	Severe acute respiratory syndrome virus; Replication; Ubiquitin; Multicatalytic endopeptidase complex; Protein; Severe acute respiratory syndrome
EG :	Coronavirus; Coronaviridae; Nidovirales; Virus; Peptidases; Hydrolases; Enzyme; Respiratory disease; Viral disease; Infection; Lung disease
SD :	Severe acute respiratory syndrome virus; Replicación; Ubiquitina; Multicatalytic endopeptidase complex; Proteína; Síndrome respiratorio agudo severo
LO :	INIST-26816.354000191918740080
ID :	11-0108132

Links to Exploration step

Pascal:11-0108132

Le document en format XML

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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">SARS coronavirus 8b reduces viral replication by down-regulating E via an ubiquitin-independent proteasome pathway</title>
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<author><name sortKey="Peiris, J S Malik" sort="Peiris, J S Malik" uniqKey="Peiris J" first="J. S. Malik" last="Peiris">J. S. Malik Peiris</name>
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<author><name sortKey="Mirazimi, Ali" sort="Mirazimi, Ali" uniqKey="Mirazimi A" first="Ali" last="Mirazimi">Ali Mirazimi</name>
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<s3>SWE</s3>
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<author><name sortKey="Tan, Yee Joo" sort="Tan, Yee Joo" uniqKey="Tan Y" first="Yee-Joo" last="Tan">Yee-Joo Tan</name>
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<series><title level="j" type="main">Microbes and infection</title>
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<front><div type="abstract" xml:lang="en">The severe acute respiratory syndrome coronavirus (SARS-CoV) 8b protein, which is not expressed by other known coronaviruses, can down-regulate the envelope (E) protein via a proteasome-dependent pathway. Here, we showed that the down-regulation of E is not dependent on the lysine residues on 8b and the reduction of polyubiquitination of E mutants is not correlated with their down-regulation by 8b, suggesting an ubiquitin-independent proteasome pathway is involved. A time-course study revealed that 8b was expressed at late-stages of SARS-CoV infection. By using Vero E6 cells stably expressing green fluorescence protein-tagged 8b, ectopic expression of 8b was shown to significantly reduce the production of progeny virus and down-regulate E expression. Taken together, these results suggest that 8b negatively modulates virus replication by down-regulating E via an ubiquitin-independent proteasome pathway.</div>
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<sZ>7 aut.</sZ>
</fA14>
<fA14 i1="02"><s1>Center for Microbiological Preparedness, Swedish Institute for Infectious Disease Control</s1>
<s2>171 82 Solna</s2>
<s3>SWE</s3>
<sZ>2 aut.</sZ>
<sZ>6 aut.</sZ>
</fA14>
<fA14 i1="03"><s1>Department of Microbiology, Tumor and Cell Biology, Karolinska Institute</s1>
<s2>Stockholm</s2>
<s3>SWE</s3>
<sZ>2 aut.</sZ>
<sZ>6 aut.</sZ>
</fA14>
<fA14 i1="04"><s1>Department of Microbiology, The University of Hong Kong</s1>
<s3>HKG</s3>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
</fA14>
<fA14 i1="05"><s1>HKU-Pasteur Research Centre</s1>
<s3>HKG</s3>
<sZ>5 aut.</sZ>
</fA14>
<fA14 i1="06"><s1>Department of Microbiology, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore</s1>
<s3>SGP</s3>
<sZ>7 aut.</sZ>
</fA14>
<fA20><s1>179-188</s1>
</fA20>
<fA21><s1>2011</s1>
</fA21>
<fA23 i1="01"><s0>ENG</s0>
</fA23>
<fA43 i1="01"><s1>INIST</s1>
<s2>26816</s2>
<s5>354000191918740080</s5>
</fA43>
<fA44><s0>0000</s0>
<s1>© 2011 INIST-CNRS. All rights reserved.</s1>
</fA44>
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</fA45>
<fA47 i1="01" i2="1"><s0>11-0108132</s0>
</fA47>
<fA60><s1>P</s1>
</fA60>
<fA61><s0>A</s0>
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<fA64 i1="01" i2="1"><s0>Microbes and infection</s0>
</fA64>
<fA66 i1="01"><s0>GBR</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>The severe acute respiratory syndrome coronavirus (SARS-CoV) 8b protein, which is not expressed by other known coronaviruses, can down-regulate the envelope (E) protein via a proteasome-dependent pathway. Here, we showed that the down-regulation of E is not dependent on the lysine residues on 8b and the reduction of polyubiquitination of E mutants is not correlated with their down-regulation by 8b, suggesting an ubiquitin-independent proteasome pathway is involved. A time-course study revealed that 8b was expressed at late-stages of SARS-CoV infection. By using Vero E6 cells stably expressing green fluorescence protein-tagged 8b, ectopic expression of 8b was shown to significantly reduce the production of progeny virus and down-regulate E expression. Taken together, these results suggest that 8b negatively modulates virus replication by down-regulating E via an ubiquitin-independent proteasome pathway.</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>002A05</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Virus syndrome respiratoire aigu sévère</s0>
<s2>NW</s2>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>Severe acute respiratory syndrome virus</s0>
<s2>NW</s2>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Severe acute respiratory syndrome virus</s0>
<s2>NW</s2>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Réplication</s0>
<s5>05</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Replication</s0>
<s5>05</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Replicación</s0>
<s5>05</s5>
</fC03>
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<s5>06</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Ubiquitin</s0>
<s5>06</s5>
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<fC03 i1="04" i2="X" l="FRE"><s0>Multicatalytic endopeptidase complex</s0>
<s2>FE</s2>
<s5>07</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Multicatalytic endopeptidase complex</s0>
<s2>FE</s2>
<s5>07</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Multicatalytic endopeptidase complex</s0>
<s2>FE</s2>
<s5>07</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Protéine</s0>
<s5>08</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Protein</s0>
<s5>08</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Proteína</s0>
<s5>08</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Syndrome respiratoire aigu sévère</s0>
<s2>NM</s2>
<s5>14</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Severe acute respiratory syndrome</s0>
<s2>NM</s2>
<s5>14</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Síndrome respiratorio agudo severo</s0>
<s2>NM</s2>
<s5>14</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Coronavirus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Coronavirus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Coronavirus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
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<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Peptidases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Peptidases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Peptidases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Hydrolases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="06" i2="X" l="ENG"><s0>Hydrolases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="06" i2="X" l="SPA"><s0>Hydrolases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="07" i2="X" l="FRE"><s0>Enzyme</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="07" i2="X" l="ENG"><s0>Enzyme</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="07" i2="X" l="SPA"><s0>Enzima</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="08" i2="X" l="FRE"><s0>Pathologie de l'appareil respiratoire</s0>
<s5>13</s5>
</fC07>
<fC07 i1="08" i2="X" l="ENG"><s0>Respiratory disease</s0>
<s5>13</s5>
</fC07>
<fC07 i1="08" i2="X" l="SPA"><s0>Aparato respiratorio patología</s0>
<s5>13</s5>
</fC07>
<fC07 i1="09" i2="X" l="FRE"><s0>Virose</s0>
</fC07>
<fC07 i1="09" i2="X" l="ENG"><s0>Viral disease</s0>
</fC07>
<fC07 i1="09" i2="X" l="SPA"><s0>Virosis</s0>
</fC07>
<fC07 i1="10" i2="X" l="FRE"><s0>Infection</s0>
</fC07>
<fC07 i1="10" i2="X" l="ENG"><s0>Infection</s0>
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<fC07 i1="10" i2="X" l="SPA"><s0>Infección</s0>
</fC07>
<fC07 i1="11" i2="X" l="FRE"><s0>Pathologie des poumons</s0>
<s5>16</s5>
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<fC07 i1="11" i2="X" l="ENG"><s0>Lung disease</s0>
<s5>16</s5>
</fC07>
<fC07 i1="11" i2="X" l="SPA"><s0>Pulmón patología</s0>
<s5>16</s5>
</fC07>
<fN21><s1>073</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
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<server><NO>PASCAL 11-0108132 INIST</NO>
<ET>SARS coronavirus 8b reduces viral replication by down-regulating E via an ubiquitin-independent proteasome pathway</ET>
<AU>KENG (Choong-Tat); AKERSTRÖM (Sara); LEUNG (Cynthia Sau-Wai); POON (Leo L. M.); PEIRIS (J. S. Malik); MIRAZIMI (Ali); TAN (Yee-Joo)</AU>
<AF>Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology/Singapour (1 aut., 7 aut.); Center for Microbiological Preparedness, Swedish Institute for Infectious Disease Control/171 82 Solna/Suède (2 aut., 6 aut.); Department of Microbiology, Tumor and Cell Biology, Karolinska Institute/Stockholm/Suède (2 aut., 6 aut.); Department of Microbiology, The University of Hong Kong/Hong-Kong (3 aut., 4 aut., 5 aut.); HKU-Pasteur Research Centre/Hong-Kong (5 aut.); Department of Microbiology, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore/Singapour (7 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Microbes and infection; ISSN 1286-4579; Royaume-Uni; Da. 2011; Vol. 13; No. 2; Pp. 179-188; Bibl. 44 ref.</SO>
<LA>Anglais</LA>
<EA>The severe acute respiratory syndrome coronavirus (SARS-CoV) 8b protein, which is not expressed by other known coronaviruses, can down-regulate the envelope (E) protein via a proteasome-dependent pathway. Here, we showed that the down-regulation of E is not dependent on the lysine residues on 8b and the reduction of polyubiquitination of E mutants is not correlated with their down-regulation by 8b, suggesting an ubiquitin-independent proteasome pathway is involved. A time-course study revealed that 8b was expressed at late-stages of SARS-CoV infection. By using Vero E6 cells stably expressing green fluorescence protein-tagged 8b, ectopic expression of 8b was shown to significantly reduce the production of progeny virus and down-regulate E expression. Taken together, these results suggest that 8b negatively modulates virus replication by down-regulating E via an ubiquitin-independent proteasome pathway.</EA>
<CC>002A05</CC>
<FD>Virus syndrome respiratoire aigu sévère; Réplication; Ubiquitine; Multicatalytic endopeptidase complex; Protéine; Syndrome respiratoire aigu sévère</FD>
<FG>Coronavirus; Coronaviridae; Nidovirales; Virus; Peptidases; Hydrolases; Enzyme; Pathologie de l'appareil respiratoire; Virose; Infection; Pathologie des poumons</FG>
<ED>Severe acute respiratory syndrome virus; Replication; Ubiquitin; Multicatalytic endopeptidase complex; Protein; Severe acute respiratory syndrome</ED>
<EG>Coronavirus; Coronaviridae; Nidovirales; Virus; Peptidases; Hydrolases; Enzyme; Respiratory disease; Viral disease; Infection; Lung disease</EG>
<SD>Severe acute respiratory syndrome virus; Replicación; Ubiquitina; Multicatalytic endopeptidase complex; Proteína; Síndrome respiratorio agudo severo</SD>
<LO>INIST-26816.354000191918740080</LO>
<ID>11-0108132</ID>
</server>
</inist>
</record>

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SARS coronavirus 8b reduces viral replication by down-regulating E via an ubiquitin-independent proteasome pathway

SARS coronavirus 8b reduces viral replication by down-regulating E via an ubiquitin-independent proteasome pathway

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