Antibody to severe acute respiratory syndrome (SARS)-associated coronavirus spike protein domain 2 cross-reacts with lung epithelial cells and causes cytotoxicity
Identifieur interne : 000705 ( PascalFrancis/Checkpoint ); précédent : 000704; suivant : 000706Antibody to severe acute respiratory syndrome (SARS)-associated coronavirus spike protein domain 2 cross-reacts with lung epithelial cells and causes cytotoxicity
Auteurs : Y. S. Lin [Taïwan] ; C. F. Lin [Taïwan] ; Y. T. Fang [Taïwan] ; Y. M. Kuo [Taïwan] ; P. C. Liao [Taïwan] ; T. M. Yeh [Taïwan] ; K. Y. Hwa [Taïwan] ; C. C. K. Shieh [Taïwan] ; J. H. Yen [Taïwan] ; H. J. Wang [Taïwan] ; I. J. Su [Taïwan] ; H. Y. Lei [Taïwan]Source :
- Clinical and experimental immunology : (Print) [ 0009-9104 ] ; 2005.
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Abstract
Both viral effect and immune-mediated mechanism are involved in the pathogenesis of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) infection. In this study, we showed that in SARS patient sera there were autoantibodies (autoAbs) that reacted with A549 cells, the type-2 pneumocytes, and that these autoAbs were mainly IgG. The autoAbs were detectable 20 days after fever onset. Tests of non-SARS-pneumonia patients did not show the same autoAb production as in SARS patients. After sera IgG bound to A549 cells, cytotoxicity was induced. Cell cytotoxicity and the anti-epithelial cell IgG level were positively correlated. Preabsorption and binding assays indicated the existence of cross-reactive epitopes on SARS-CoV spike protein domain 2 (S2). Furthermore, treatment of A549 cells with anti-S2 Abs and IFN-γ resulted in an increase in the adherence of human peripheral blood mononuclear cells to these epithelial cells. Taken together, we have demonstrated that the anti-S2 Abs in SARS patient sera cause cytotoxic injury as well as enhance immune cell adhesion to epithelial cells. The onset of autoimmune responses in SARS-CoV infection may be implicated in SARS pathogenesis.
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Antibody to severe acute respiratory syndrome (SARS)-associated coronavirus spike protein domain 2 cross-reacts with lung epithelial cells and causes cytotoxicity</title><author><name sortKey="Lin, Y S" sort="Lin, Y S" uniqKey="Lin Y" first="Y. S." last="Lin">Y. S. Lin</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology and Immunology, National Cheng Kung University Medical College</s1><s2>Tainan</s2><s3>TWN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Tainan</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="08"><s1>ScinoPharm Biotech Ltd</s1><s2>Tainan</s2><s3>TWN</s3></inist:fA14><country>Taïwan</country><wicri:noRegion>ScinoPharm Biotech Ltd</wicri:noRegion></affiliation></author><author><name sortKey="Lin, C F" sort="Lin, C F" uniqKey="Lin C" first="C. F." last="Lin">C. F. Lin</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology and Immunology, National Cheng Kung University Medical College</s1><s2>Tainan</s2><s3>TWN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Tainan</wicri:noRegion></affiliation></author><author><name sortKey="Fang, Y T" sort="Fang, Y T" uniqKey="Fang Y" first="Y. T." last="Fang">Y. T. Fang</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology and Immunology, National Cheng Kung University Medical College</s1><s2>Tainan</s2><s3>TWN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Tainan</wicri:noRegion></affiliation></author><author><name sortKey="Kuo, Y M" sort="Kuo, Y M" uniqKey="Kuo Y" first="Y. M." last="Kuo">Y. M. Kuo</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Cell Biology and Anatomy, National Cheng Kung University Medical College</s1><s2>Tainan</s2><s3>TWN</s3><sZ>4 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Tainan</wicri:noRegion></affiliation></author><author><name sortKey="Liao, P C" sort="Liao, P C" uniqKey="Liao P" first="P. C." last="Liao">P. C. Liao</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Departmnt of Environmental and Occupational Health, National Cheng Kung University Medical College</s1><s2>Tainan</s2><s3>TWN</s3><sZ>5 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Tainan</wicri:noRegion></affiliation></author><author><name sortKey="Yeh, T M" sort="Yeh, T M" uniqKey="Yeh T" first="T. M." last="Yeh">T. M. Yeh</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Department of Medical Technology, National Cheng Kung University Medical College</s1><s2>Tainan</s2><s3>TWN</s3><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Tainan</wicri:noRegion></affiliation></author><author><name sortKey="Hwa, K Y" sort="Hwa, K Y" uniqKey="Hwa K" first="K. Y." last="Hwa">K. Y. Hwa</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Department of Medical Technology, National Cheng Kung University Medical College</s1><s2>Tainan</s2><s3>TWN</s3><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Tainan</wicri:noRegion></affiliation></author><author><name sortKey="Shieh, C C K" sort="Shieh, C C K" uniqKey="Shieh C" first="C. C. K." last="Shieh">C. C. K. Shieh</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology and Immunology, National Cheng Kung University Medical College</s1><s2>Tainan</s2><s3>TWN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Tainan</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="05"><s1>Department of Paediatrics, National Cheng Kung University Medical College</s1><s2>Tainan</s2><s3>TWN</s3><sZ>8 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Tainan</wicri:noRegion></affiliation></author><author><name sortKey="Yen, J H" sort="Yen, J H" uniqKey="Yen J" first="J. H." last="Yen">J. H. Yen</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Departmnt of Environmental and Occupational Health, National Cheng Kung University Medical College</s1><s2>Tainan</s2><s3>TWN</s3><sZ>5 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Tainan</wicri:noRegion></affiliation></author><author><name sortKey="Wang, H J" sort="Wang, H J" uniqKey="Wang H" first="H. J." last="Wang">H. J. Wang</name><affiliation wicri:level="1"><inist:fA14 i1="06"><s1>Division of Clinical Research, National Health Research Institutes</s1><s2>Tainan</s2><s3>TWN</s3><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Tainan</wicri:noRegion></affiliation></author><author><name sortKey="Su, I J" sort="Su, I J" uniqKey="Su I" first="I. J." last="Su">I. J. Su</name><affiliation wicri:level="1"><inist:fA14 i1="06"><s1>Division of Clinical Research, National Health Research Institutes</s1><s2>Tainan</s2><s3>TWN</s3><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Tainan</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="07"><s1>Centre for Disease Control, Department of Health</s1><s2>Taipei</s2><s3>TWN</s3><sZ>11 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author><author><name sortKey="Lei, H Y" sort="Lei, H Y" uniqKey="Lei H" first="H. Y." last="Lei">H. Y. Lei</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology and Immunology, National Cheng Kung University Medical College</s1><s2>Tainan</s2><s3>TWN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Tainan</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">05-0334001</idno><date when="2005">2005</date><idno type="stanalyst">PASCAL 05-0334001 INIST</idno><idno type="RBID">Pascal:05-0334001</idno><idno type="wicri:Area/PascalFrancis/Corpus">000636</idno><idno type="wicri:Area/PascalFrancis/Curation">000354</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000705</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000705</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Antibody to severe acute respiratory syndrome (SARS)-associated coronavirus spike protein domain 2 cross-reacts with lung epithelial cells and causes cytotoxicity</title><author><name sortKey="Lin, Y S" sort="Lin, Y S" uniqKey="Lin Y" first="Y. S." last="Lin">Y. S. Lin</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology and Immunology, National Cheng Kung University Medical College</s1><s2>Tainan</s2><s3>TWN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Tainan</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="08"><s1>ScinoPharm Biotech Ltd</s1><s2>Tainan</s2><s3>TWN</s3></inist:fA14><country>Taïwan</country><wicri:noRegion>ScinoPharm Biotech Ltd</wicri:noRegion></affiliation></author><author><name sortKey="Lin, C F" sort="Lin, C F" uniqKey="Lin C" first="C. F." last="Lin">C. F. Lin</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology and Immunology, National Cheng Kung University Medical College</s1><s2>Tainan</s2><s3>TWN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Tainan</wicri:noRegion></affiliation></author><author><name sortKey="Fang, Y T" sort="Fang, Y T" uniqKey="Fang Y" first="Y. T." last="Fang">Y. T. Fang</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology and Immunology, National Cheng Kung University Medical College</s1><s2>Tainan</s2><s3>TWN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Tainan</wicri:noRegion></affiliation></author><author><name sortKey="Kuo, Y M" sort="Kuo, Y M" uniqKey="Kuo Y" first="Y. M." last="Kuo">Y. M. Kuo</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Cell Biology and Anatomy, National Cheng Kung University Medical College</s1><s2>Tainan</s2><s3>TWN</s3><sZ>4 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Tainan</wicri:noRegion></affiliation></author><author><name sortKey="Liao, P C" sort="Liao, P C" uniqKey="Liao P" first="P. C." last="Liao">P. C. Liao</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Departmnt of Environmental and Occupational Health, National Cheng Kung University Medical College</s1><s2>Tainan</s2><s3>TWN</s3><sZ>5 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Tainan</wicri:noRegion></affiliation></author><author><name sortKey="Yeh, T M" sort="Yeh, T M" uniqKey="Yeh T" first="T. M." last="Yeh">T. M. Yeh</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Department of Medical Technology, National Cheng Kung University Medical College</s1><s2>Tainan</s2><s3>TWN</s3><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Tainan</wicri:noRegion></affiliation></author><author><name sortKey="Hwa, K Y" sort="Hwa, K Y" uniqKey="Hwa K" first="K. Y." last="Hwa">K. Y. Hwa</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Department of Medical Technology, National Cheng Kung University Medical College</s1><s2>Tainan</s2><s3>TWN</s3><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Tainan</wicri:noRegion></affiliation></author><author><name sortKey="Shieh, C C K" sort="Shieh, C C K" uniqKey="Shieh C" first="C. C. K." last="Shieh">C. C. K. Shieh</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology and Immunology, National Cheng Kung University Medical College</s1><s2>Tainan</s2><s3>TWN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Tainan</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="05"><s1>Department of Paediatrics, National Cheng Kung University Medical College</s1><s2>Tainan</s2><s3>TWN</s3><sZ>8 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Tainan</wicri:noRegion></affiliation></author><author><name sortKey="Yen, J H" sort="Yen, J H" uniqKey="Yen J" first="J. H." last="Yen">J. H. Yen</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Departmnt of Environmental and Occupational Health, National Cheng Kung University Medical College</s1><s2>Tainan</s2><s3>TWN</s3><sZ>5 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Tainan</wicri:noRegion></affiliation></author><author><name sortKey="Wang, H J" sort="Wang, H J" uniqKey="Wang H" first="H. J." last="Wang">H. J. Wang</name><affiliation wicri:level="1"><inist:fA14 i1="06"><s1>Division of Clinical Research, National Health Research Institutes</s1><s2>Tainan</s2><s3>TWN</s3><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Tainan</wicri:noRegion></affiliation></author><author><name sortKey="Su, I J" sort="Su, I J" uniqKey="Su I" first="I. J." last="Su">I. J. Su</name><affiliation wicri:level="1"><inist:fA14 i1="06"><s1>Division of Clinical Research, National Health Research Institutes</s1><s2>Tainan</s2><s3>TWN</s3><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Tainan</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="07"><s1>Centre for Disease Control, Department of Health</s1><s2>Taipei</s2><s3>TWN</s3><sZ>11 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author><author><name sortKey="Lei, H Y" sort="Lei, H Y" uniqKey="Lei H" first="H. Y." last="Lei">H. Y. Lei</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology and Immunology, National Cheng Kung University Medical College</s1><s2>Tainan</s2><s3>TWN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Tainan</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Clinical and experimental immunology : (Print)</title><title level="j" type="abbreviated">Clin. exp. immunol. : (Print)</title><idno type="ISSN">0009-9104</idno><imprint><date when="2005">2005</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Clinical and experimental immunology : (Print)</title><title level="j" type="abbreviated">Clin. exp. immunol. : (Print)</title><idno type="ISSN">0009-9104</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibody</term><term>Autoantibody</term><term>Autoimmunity</term><term>Coronavirus</term><term>Cytotoxicity</term><term>Epithelial cell</term><term>Protein</term><term>Severe acute respiratory syndrome</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term><term>Anticorps</term><term>Coronavirus</term><term>Autoimmunité</term><term>Protéine</term><term>Cellule épithéliale</term><term>Cytotoxicité</term><term>Autoanticorps</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Both viral effect and immune-mediated mechanism are involved in the pathogenesis of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) infection. In this study, we showed that in SARS patient sera there were autoantibodies (autoAbs) that reacted with A549 cells, the type-2 pneumocytes, and that these autoAbs were mainly IgG. The autoAbs were detectable 20 days after fever onset. Tests of non-SARS-pneumonia patients did not show the same autoAb production as in SARS patients. After sera IgG bound to A549 cells, cytotoxicity was induced. Cell cytotoxicity and the anti-epithelial cell IgG level were positively correlated. Preabsorption and binding assays indicated the existence of cross-reactive epitopes on SARS-CoV spike protein domain 2 (S2). Furthermore, treatment of A549 cells with anti-S2 Abs and IFN-γ resulted in an increase in the adherence of human peripheral blood mononuclear cells to these epithelial cells. Taken together, we have demonstrated that the anti-S2 Abs in SARS patient sera cause cytotoxic injury as well as enhance immune cell adhesion to epithelial cells. The onset of autoimmune responses in SARS-CoV infection may be implicated in SARS pathogenesis.</div></front></TEI><inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0009-9104</s0></fA01><fA02 i1="01"><s0>CEXIAL</s0></fA02><fA03 i2="1"><s0>Clin. exp. immunol. : (Print)</s0></fA03><fA05><s2>141</s2></fA05><fA06><s2>3</s2></fA06><fA08 i1="01" i2="1" l="ENG"><s1>Antibody to severe acute respiratory syndrome (SARS)-associated coronavirus spike protein domain 2 cross-reacts with lung epithelial cells and causes cytotoxicity</s1></fA08><fA11 i1="01" i2="1"><s1>LIN (Y. S.)</s1></fA11><fA11 i1="02" i2="1"><s1>LIN (C. F.)</s1></fA11><fA11 i1="03" i2="1"><s1>FANG (Y. T.)</s1></fA11><fA11 i1="04" i2="1"><s1>KUO (Y. M.)</s1></fA11><fA11 i1="05" i2="1"><s1>LIAO (P. C.)</s1></fA11><fA11 i1="06" i2="1"><s1>YEH (T. M.)</s1></fA11><fA11 i1="07" i2="1"><s1>HWA (K. Y.)</s1></fA11><fA11 i1="08" i2="1"><s1>SHIEH (C. C. K.)</s1></fA11><fA11 i1="09" i2="1"><s1>YEN (J. H.)</s1></fA11><fA11 i1="10" i2="1"><s1>WANG (H. J.)</s1></fA11><fA11 i1="11" i2="1"><s1>SU (I. J.)</s1></fA11><fA11 i1="12" i2="1"><s1>LEI (H. Y.)</s1></fA11><fA14 i1="01"><s1>Department of Microbiology and Immunology, National Cheng Kung University Medical College</s1><s2>Tainan</s2><s3>TWN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ></fA14><fA14 i1="02"><s1>Department of Cell Biology and Anatomy, National Cheng Kung University Medical College</s1><s2>Tainan</s2><s3>TWN</s3><sZ>4 aut.</sZ></fA14><fA14 i1="03"><s1>Departmnt of Environmental and Occupational Health, National Cheng Kung University Medical College</s1><s2>Tainan</s2><s3>TWN</s3><sZ>5 aut.</sZ><sZ>9 aut.</sZ></fA14><fA14 i1="04"><s1>Department of Medical Technology, National Cheng Kung University Medical College</s1><s2>Tainan</s2><s3>TWN</s3><sZ>6 aut.</sZ><sZ>7 aut.</sZ></fA14><fA14 i1="05"><s1>Department of Paediatrics, National Cheng Kung University Medical College</s1><s2>Tainan</s2><s3>TWN</s3><sZ>8 aut.</sZ></fA14><fA14 i1="06"><s1>Division of Clinical Research, National Health Research Institutes</s1><s2>Tainan</s2><s3>TWN</s3><sZ>10 aut.</sZ><sZ>11 aut.</sZ></fA14><fA14 i1="07"><s1>Centre for Disease Control, Department of Health</s1><s2>Taipei</s2><s3>TWN</s3><sZ>11 aut.</sZ></fA14><fA14 i1="08"><s1>ScinoPharm Biotech Ltd</s1><s2>Tainan</s2><s3>TWN</s3></fA14><fA20><s1>500-508</s1></fA20><fA21><s1>2005</s1></fA21><fA23 i1="01"><s0>ENG</s0></fA23><fA43 i1="01"><s1>INIST</s1><s2>12690</s2><s5>354000137997970150</s5></fA43><fA44><s0>0000</s0><s1>© 2005 INIST-CNRS. All rights reserved.</s1></fA44><fA45><s0>41 ref.</s0></fA45><fA47 i1="01" i2="1"><s0>05-0334001</s0></fA47><fA60><s1>P</s1></fA60><fA61><s0>A</s0></fA61><fA64 i1="01" i2="1"><s0>Clinical and experimental immunology : (Print)</s0></fA64><fA66 i1="01"><s0>GBR</s0></fA66><fC01 i1="01" l="ENG"><s0>Both viral effect and immune-mediated mechanism are involved in the pathogenesis of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) infection. In this study, we showed that in SARS patient sera there were autoantibodies (autoAbs) that reacted with A549 cells, the type-2 pneumocytes, and that these autoAbs were mainly IgG. The autoAbs were detectable 20 days after fever onset. Tests of non-SARS-pneumonia patients did not show the same autoAb production as in SARS patients. After sera IgG bound to A549 cells, cytotoxicity was induced. Cell cytotoxicity and the anti-epithelial cell IgG level were positively correlated. Preabsorption and binding assays indicated the existence of cross-reactive epitopes on SARS-CoV spike protein domain 2 (S2). Furthermore, treatment of A549 cells with anti-S2 Abs and IFN-γ resulted in an increase in the adherence of human peripheral blood mononuclear cells to these epithelial cells. Taken together, we have demonstrated that the anti-S2 Abs in SARS patient sera cause cytotoxic injury as well as enhance immune cell adhesion to epithelial cells. The onset of autoimmune responses in SARS-CoV infection may be implicated in SARS pathogenesis.</s0></fC01><fC02 i1="01" i2="X"><s0>002B06</s0></fC02><fC02 i1="02" i2="X"><s0>002A06</s0></fC02><fC03 i1="01" i2="X" l="FRE"><s0>Syndrome respiratoire aigu sévère</s0><s2>NM</s2><s5>01</s5></fC03><fC03 i1="01" i2="X" l="ENG"><s0>Severe acute respiratory syndrome</s0><s2>NM</s2><s5>01</s5></fC03><fC03 i1="01" i2="X" l="SPA"><s0>Síndrome respiratorio agudo severo</s0><s2>NM</s2><s5>01</s5></fC03><fC03 i1="02" i2="X" l="FRE"><s0>Anticorps</s0><s5>02</s5></fC03><fC03 i1="02" i2="X" l="ENG"><s0>Antibody</s0><s5>02</s5></fC03><fC03 i1="02" i2="X" l="SPA"><s0>Anticuerpo</s0><s5>02</s5></fC03><fC03 i1="03" i2="X" l="FRE"><s0>Coronavirus</s0><s2>NW</s2><s5>03</s5></fC03><fC03 i1="03" i2="X" l="ENG"><s0>Coronavirus</s0><s2>NW</s2><s5>03</s5></fC03><fC03 i1="03" i2="X" l="SPA"><s0>Coronavirus</s0><s2>NW</s2><s5>03</s5></fC03><fC03 i1="04" i2="X" l="FRE"><s0>Autoimmunité</s0><s5>04</s5></fC03><fC03 i1="04" i2="X" l="ENG"><s0>Autoimmunity</s0><s5>04</s5></fC03><fC03 i1="04" i2="X" l="SPA"><s0>Autoinmunidad</s0><s5>04</s5></fC03><fC03 i1="05" i2="X" l="FRE"><s0>Protéine</s0><s5>05</s5></fC03><fC03 i1="05" i2="X" l="ENG"><s0>Protein</s0><s5>05</s5></fC03><fC03 i1="05" i2="X" l="SPA"><s0>Proteína</s0><s5>05</s5></fC03><fC03 i1="06" i2="X" l="FRE"><s0>Cellule épithéliale</s0><s5>06</s5></fC03><fC03 i1="06" i2="X" l="ENG"><s0>Epithelial cell</s0><s5>06</s5></fC03><fC03 i1="06" i2="X" l="SPA"><s0>Célula epitelial</s0><s5>06</s5></fC03><fC03 i1="07" i2="X" l="FRE"><s0>Cytotoxicité</s0><s5>08</s5></fC03><fC03 i1="07" i2="X" l="ENG"><s0>Cytotoxicity</s0><s5>08</s5></fC03><fC03 i1="07" i2="X" l="SPA"><s0>Citotoxicidad</s0><s5>08</s5></fC03><fC03 i1="08" i2="X" l="FRE"><s0>Autoanticorps</s0><s5>09</s5></fC03><fC03 i1="08" i2="X" l="ENG"><s0>Autoantibody</s0><s5>09</s5></fC03><fC03 i1="08" i2="X" l="SPA"><s0>Autoanticuerpo</s0><s5>09</s5></fC03><fC07 i1="01" i2="X" l="FRE"><s0>Virose</s0></fC07><fC07 i1="01" i2="X" l="ENG"><s0>Viral disease</s0></fC07><fC07 i1="01" i2="X" l="SPA"><s0>Virosis</s0></fC07><fC07 i1="02" i2="X" l="FRE"><s0>Infection</s0></fC07><fC07 i1="02" i2="X" l="ENG"><s0>Infection</s0></fC07><fC07 i1="02" i2="X" l="SPA"><s0>Infección</s0></fC07><fC07 i1="03" i2="X" l="FRE"><s0>Coronaviridae</s0><s2>NW</s2></fC07><fC07 i1="03" i2="X" l="ENG"><s0>Coronaviridae</s0><s2>NW</s2></fC07><fC07 i1="03" i2="X" l="SPA"><s0>Coronaviridae</s0><s2>NW</s2></fC07><fC07 i1="04" i2="X" l="FRE"><s0>Nidovirales</s0><s2>NW</s2></fC07><fC07 i1="04" i2="X" l="ENG"><s0>Nidovirales</s0><s2>NW</s2></fC07><fC07 i1="04" i2="X" l="SPA"><s0>Nidovirales</s0><s2>NW</s2></fC07><fC07 i1="05" i2="X" l="FRE"><s0>Virus</s0><s2>NW</s2></fC07><fC07 i1="05" i2="X" l="ENG"><s0>Virus</s0><s2>NW</s2></fC07><fC07 i1="05" i2="X" l="SPA"><s0>Virus</s0><s2>NW</s2></fC07><fC07 i1="06" i2="X" l="FRE"><s0>Poumon pathologie</s0><s5>37</s5></fC07><fC07 i1="06" i2="X" l="ENG"><s0>Lung disease</s0><s5>37</s5></fC07><fC07 i1="06" i2="X" l="SPA"><s0>Pulmón patología</s0><s5>37</s5></fC07><fC07 i1="07" i2="X" l="FRE"><s0>Immunologie</s0><s5>38</s5></fC07><fC07 i1="07" i2="X" l="ENG"><s0>Immunology</s0><s5>38</s5></fC07><fC07 i1="07" i2="X" l="SPA"><s0>Inmunología</s0><s5>38</s5></fC07><fC07 i1="08" i2="X" l="FRE"><s0>Immunopathologie</s0><s5>39</s5></fC07><fC07 i1="08" i2="X" l="ENG"><s0>Immunopathology</s0><s5>39</s5></fC07><fC07 i1="08" i2="X" l="SPA"><s0>Inmunopatología</s0><s5>39</s5></fC07><fC07 i1="09" i2="X" l="FRE"><s0>Appareil respiratoire pathologie</s0><s5>40</s5></fC07><fC07 i1="09" i2="X" l="ENG"><s0>Respiratory disease</s0><s5>40</s5></fC07><fC07 i1="09" i2="X" l="SPA"><s0>Aparato respiratorio patología</s0><s5>40</s5></fC07><fN21><s1>234</s1></fN21><fN44 i1="01"><s1>OTO</s1></fN44><fN82><s1>OTO</s1></fN82></pA></standard></inist><affiliations><list><country><li>Taïwan</li></country></list><tree><country name="Taïwan"><noRegion><name sortKey="Lin, Y S" sort="Lin, Y S" uniqKey="Lin Y" first="Y. S." last="Lin">Y. S. Lin</name></noRegion><name sortKey="Fang, Y T" sort="Fang, Y T" uniqKey="Fang Y" first="Y. T." last="Fang">Y. T. Fang</name><name sortKey="Hwa, K Y" sort="Hwa, K Y" uniqKey="Hwa K" first="K. Y." last="Hwa">K. Y. Hwa</name><name sortKey="Kuo, Y M" sort="Kuo, Y M" uniqKey="Kuo Y" first="Y. M." last="Kuo">Y. M. Kuo</name><name sortKey="Lei, H Y" sort="Lei, H Y" uniqKey="Lei H" first="H. Y." last="Lei">H. Y. Lei</name><name sortKey="Liao, P C" sort="Liao, P C" uniqKey="Liao P" first="P. C." last="Liao">P. C. Liao</name><name sortKey="Lin, C F" sort="Lin, C F" uniqKey="Lin C" first="C. F." last="Lin">C. F. Lin</name><name sortKey="Lin, Y S" sort="Lin, Y S" uniqKey="Lin Y" first="Y. S." last="Lin">Y. S. Lin</name><name sortKey="Shieh, C C K" sort="Shieh, C C K" uniqKey="Shieh C" first="C. C. K." last="Shieh">C. C. K. Shieh</name><name sortKey="Shieh, C C K" sort="Shieh, C C K" uniqKey="Shieh C" first="C. C. K." last="Shieh">C. C. K. Shieh</name><name sortKey="Su, I J" sort="Su, I J" uniqKey="Su I" first="I. J." last="Su">I. J. Su</name><name sortKey="Su, I J" sort="Su, I J" uniqKey="Su I" first="I. J." last="Su">I. J. Su</name><name sortKey="Wang, H J" sort="Wang, H J" uniqKey="Wang H" first="H. J." last="Wang">H. J. Wang</name><name sortKey="Yeh, T M" sort="Yeh, T M" uniqKey="Yeh T" first="T. M." last="Yeh">T. M. Yeh</name><name sortKey="Yen, J H" sort="Yen, J H" uniqKey="Yen J" first="J. H." last="Yen">J. H. Yen</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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