SrasV1, PascalFrancis, Checkpoint, bibRecord, 000656

Evaluation of a recombinant nucleocapsid protein-based assay for anti-SARS-CoV IgG detection

Identifieur interne : 000656 ( PascalFrancis/Checkpoint ); précédent : 000655; suivant : 000657

Evaluation of a recombinant nucleocapsid protein-based assay for anti-SARS-CoV IgG detection

Auteurs : Paul K. S. Chan [Hong Kong] ; Esther Y. M. Liu [Hong Kong] ; Danny T. M. Leung [Hong Kong] ; Jo L. K. Cheung [Hong Kong] ; C. H. Ma [Hong Kong] ; Frankie C. H. Tam [Hong Kong] ; Mamie Hui [Hong Kong] ; John S. Tam [Hong Kong] ; PAK LEONG LIM [Hong Kong]

Source :

Journal of medical virology [ 0146-6615 ] ; 2005.

RBID : Pascal:05-0458730

Descripteurs français

Pascal (Inist)
- Coronavirus, Protéine recombinante, Nucléocapside, IgG, Détection, Diagnostic, Méthode immunoenzymatique, Enzyme, Sérologie, Syndrome respiratoire aigu sévère.
Wicri :
- topic : Enzyme.

English descriptors

KwdEn :
- Coronavirus, Detection, Diagnosis, Enzyme, Enzyme immunoassay, IgG, Nucleocapsid, Recombinant protein, Serology, Severe acute respiratory syndrome.

Abstract

A high throughput accurate assay for anti-SARS-CoV IgG detection is needed for large-scale epidemiological studies. The evaluation of a commercial recombinant nucleocapsid protein-based microtitre plate enzyme immunoassay, ELISARS is described. The results on 150 sera from SARS patients and 450 sera from non-SARS controls showed that this assay had a high level of sensitivity (96.2% for late serum samples) and specificity (97.8%). The performance and setup of this assay fulfills the requirement as a screening test for large-scale studies. A vast majority of SARS patients developed antibodies against the nucleocapsid protein. In some patients (10/45), a high level of anti-nucleocapsid antibody appeared very early in the course of the illness. In contrast, a minority (4 of 105 patients) never developed these antibodies. The implication of differences in antibody response to the nucleocapsid protein deserves further investigation.

Affiliations:

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Pascal:05-0458730

Le document en format XML

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<term>Detection</term>
<term>Diagnosis</term>
<term>Enzyme</term>
<term>Enzyme immunoassay</term>
<term>IgG</term>
<term>Nucleocapsid</term>
<term>Recombinant protein</term>
<term>Serology</term>
<term>Severe acute respiratory syndrome</term>
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<term>Protéine recombinante</term>
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<term>IgG</term>
<term>Détection</term>
<term>Diagnostic</term>
<term>Méthode immunoenzymatique</term>
<term>Enzyme</term>
<term>Sérologie</term>
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<front><div type="abstract" xml:lang="en">A high throughput accurate assay for anti-SARS-CoV IgG detection is needed for large-scale epidemiological studies. The evaluation of a commercial recombinant nucleocapsid protein-based microtitre plate enzyme immunoassay, ELISARS is described. The results on 150 sera from SARS patients and 450 sera from non-SARS controls showed that this assay had a high level of sensitivity (96.2% for late serum samples) and specificity (97.8%). The performance and setup of this assay fulfills the requirement as a screening test for large-scale studies. A vast majority of SARS patients developed antibodies against the nucleocapsid protein. In some patients (10/45), a high level of anti-nucleocapsid antibody appeared very early in the course of the illness. In contrast, a minority (4 of 105 patients) never developed these antibodies. The implication of differences in antibody response to the nucleocapsid protein deserves further investigation.</div>
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</fA01>
<fA02 i1="01"><s0>JMVIDB</s0>
</fA02>
<fA03 i2="1"><s0>J. med. virol.</s0>
</fA03>
<fA05><s2>75</s2>
</fA05>
<fA06><s2>2</s2>
</fA06>
<fA08 i1="01" i2="1" l="ENG"><s1>Evaluation of a recombinant nucleocapsid protein-based assay for anti-SARS-CoV IgG detection</s1>
</fA08>
<fA11 i1="01" i2="1"><s1>CHAN (Paul K. S.)</s1>
</fA11>
<fA11 i1="02" i2="1"><s1>LIU (Esther Y. M.)</s1>
</fA11>
<fA11 i1="03" i2="1"><s1>LEUNG (Danny T. M.)</s1>
</fA11>
<fA11 i1="04" i2="1"><s1>CHEUNG (Jo L. K.)</s1>
</fA11>
<fA11 i1="05" i2="1"><s1>MA (C. H.)</s1>
</fA11>
<fA11 i1="06" i2="1"><s1>TAM (Frankie C. H.)</s1>
</fA11>
<fA11 i1="07" i2="1"><s1>HUI (Mamie)</s1>
</fA11>
<fA11 i1="08" i2="1"><s1>TAM (John S.)</s1>
</fA11>
<fA11 i1="09" i2="1"><s1>PAK LEONG LIM</s1>
</fA11>
<fA14 i1="01"><s1>Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Prince of Wales Hospital</s1>
<s2>Shatin, New Territories</s2>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
</fA14>
<fA14 i1="02"><s1>Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital</s1>
<s2>Shatin, New Territories</s2>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
</fA14>
<fA14 i1="03"><s1>Clinical Immunology Unit, The Chinese University of Hong Kong, Prince of Wales Hospital</s1>
<s2>Shatin, New Territories</s2>
<s3>HKG</s3>
<sZ>3 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>9 aut.</sZ>
</fA14>
<fA20><s1>181-184</s1>
</fA20>
<fA21><s1>2005</s1>
</fA21>
<fA23 i1="01"><s0>ENG</s0>
</fA23>
<fA43 i1="01"><s1>INIST</s1>
<s2>17422</s2>
<s5>354000127052660010</s5>
</fA43>
<fA44><s0>0000</s0>
<s1>© 2005 INIST-CNRS. All rights reserved.</s1>
</fA44>
<fA45><s0>24 ref.</s0>
</fA45>
<fA47 i1="01" i2="1"><s0>05-0458730</s0>
</fA47>
<fA60><s1>P</s1>
</fA60>
<fA61><s0>A</s0>
</fA61>
<fA64 i1="01" i2="1"><s0>Journal of medical virology</s0>
</fA64>
<fA66 i1="01"><s0>USA</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>A high throughput accurate assay for anti-SARS-CoV IgG detection is needed for large-scale epidemiological studies. The evaluation of a commercial recombinant nucleocapsid protein-based microtitre plate enzyme immunoassay, ELISARS is described. The results on 150 sera from SARS patients and 450 sera from non-SARS controls showed that this assay had a high level of sensitivity (96.2% for late serum samples) and specificity (97.8%). The performance and setup of this assay fulfills the requirement as a screening test for large-scale studies. A vast majority of SARS patients developed antibodies against the nucleocapsid protein. In some patients (10/45), a high level of anti-nucleocapsid antibody appeared very early in the course of the illness. In contrast, a minority (4 of 105 patients) never developed these antibodies. The implication of differences in antibody response to the nucleocapsid protein deserves further investigation.</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>002A05C10</s0>
</fC02>
<fC02 i1="02" i2="X"><s0>002B05C02J</s0>
</fC02>
<fC02 i1="03" i2="X"><s0>002A05C08</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Protéine recombinante</s0>
<s5>05</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Recombinant protein</s0>
<s5>05</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Proteína recombinante</s0>
<s5>05</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Nucléocapside</s0>
<s5>06</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Nucleocapsid</s0>
<s5>06</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Nucleocápside</s0>
<s5>06</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>IgG</s0>
<s5>07</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>IgG</s0>
<s5>07</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>IgG</s0>
<s5>07</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Détection</s0>
<s5>08</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Detection</s0>
<s5>08</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Detección</s0>
<s5>08</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Diagnostic</s0>
<s5>09</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Diagnosis</s0>
<s5>09</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Diagnóstico</s0>
<s5>09</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE"><s0>Méthode immunoenzymatique</s0>
<s5>10</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG"><s0>Enzyme immunoassay</s0>
<s5>10</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA"><s0>Método inmunoenzimático</s0>
<s5>10</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE"><s0>Enzyme</s0>
<s2>FE</s2>
<s5>11</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG"><s0>Enzyme</s0>
<s2>FE</s2>
<s5>11</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA"><s0>Enzima</s0>
<s2>FE</s2>
<s5>11</s5>
</fC03>
<fC03 i1="09" i2="X" l="FRE"><s0>Sérologie</s0>
<s5>12</s5>
</fC03>
<fC03 i1="09" i2="X" l="ENG"><s0>Serology</s0>
<s5>12</s5>
</fC03>
<fC03 i1="09" i2="X" l="SPA"><s0>Serología</s0>
<s5>12</s5>
</fC03>
<fC03 i1="10" i2="X" l="FRE"><s0>Syndrome respiratoire aigu sévère</s0>
<s2>NM</s2>
<s5>14</s5>
</fC03>
<fC03 i1="10" i2="X" l="ENG"><s0>Severe acute respiratory syndrome</s0>
<s2>NM</s2>
<s5>14</s5>
</fC03>
<fC03 i1="10" i2="X" l="SPA"><s0>Síndrome respiratorio agudo severo</s0>
<s2>NM</s2>
<s5>14</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Appareil respiratoire pathologie</s0>
<s5>13</s5>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Respiratory disease</s0>
<s5>13</s5>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Aparato respiratorio patología</s0>
<s5>13</s5>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Virose</s0>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Viral disease</s0>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Virosis</s0>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Infection</s0>
</fC07>
<fC07 i1="06" i2="X" l="ENG"><s0>Infection</s0>
</fC07>
<fC07 i1="06" i2="X" l="SPA"><s0>Infección</s0>
</fC07>
<fC07 i1="07" i2="X" l="FRE"><s0>Poumon pathologie</s0>
<s5>16</s5>
</fC07>
<fC07 i1="07" i2="X" l="ENG"><s0>Lung disease</s0>
<s5>16</s5>
</fC07>
<fC07 i1="07" i2="X" l="SPA"><s0>Pulmón patología</s0>
<s5>16</s5>
</fC07>
<fN21><s1>325</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
</standard>
</inist>
<affiliations><list><country><li>Hong Kong</li>
</country>
<settlement><li>Sha Tin</li>
</settlement>
<orgName><li>Université chinoise de Hong Kong</li>
</orgName>
</list>
<tree><country name="Hong Kong"><noRegion><name sortKey="Chan, Paul K S" sort="Chan, Paul K S" uniqKey="Chan P" first="Paul K. S." last="Chan">Paul K. S. Chan</name>
</noRegion>
<name sortKey="Chan, Paul K S" sort="Chan, Paul K S" uniqKey="Chan P" first="Paul K. S." last="Chan">Paul K. S. Chan</name>
<name sortKey="Cheung, Jo L K" sort="Cheung, Jo L K" uniqKey="Cheung J" first="Jo L. K." last="Cheung">Jo L. K. Cheung</name>
<name sortKey="Hui, Mamie" sort="Hui, Mamie" uniqKey="Hui M" first="Mamie" last="Hui">Mamie Hui</name>
<name sortKey="Hui, Mamie" sort="Hui, Mamie" uniqKey="Hui M" first="Mamie" last="Hui">Mamie Hui</name>
<name sortKey="Leung, Danny T M" sort="Leung, Danny T M" uniqKey="Leung D" first="Danny T. M." last="Leung">Danny T. M. Leung</name>
<name sortKey="Liu, Esther Y M" sort="Liu, Esther Y M" uniqKey="Liu E" first="Esther Y. M." last="Liu">Esther Y. M. Liu</name>
<name sortKey="Ma, C H" sort="Ma, C H" uniqKey="Ma C" first="C. H." last="Ma">C. H. Ma</name>
<name sortKey="Pak Leong Lim" sort="Pak Leong Lim" uniqKey="Pak Leong Lim" last="Pak Leong Lim">PAK LEONG LIM</name>
<name sortKey="Tam, Frankie C H" sort="Tam, Frankie C H" uniqKey="Tam F" first="Frankie C. H." last="Tam">Frankie C. H. Tam</name>
<name sortKey="Tam, John S" sort="Tam, John S" uniqKey="Tam J" first="John S." last="Tam">John S. Tam</name>
<name sortKey="Tam, John S" sort="Tam, John S" uniqKey="Tam J" first="John S." last="Tam">John S. Tam</name>
</country>
</tree>
</affiliations>
</record>

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Evaluation of a recombinant nucleocapsid protein-based assay for anti-SARS-CoV IgG detection

Evaluation of a recombinant nucleocapsid protein-based assay for anti-SARS-CoV IgG detection

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