SrasV1, PascalFrancis, Checkpoint, bibRecord, 000650

Expression and purification of N and E proteins from Severe Acute Respiratory Syndrome (SARS)-associated coronavirus : a comparative study

Identifieur interne : 000650 ( PascalFrancis/Checkpoint ); précédent : 000649; suivant : 000651

Expression and purification of N and E proteins from Severe Acute Respiratory Syndrome (SARS)-associated coronavirus : a comparative study

Auteurs : Chia-Wei Lai [Taïwan] ; Yao-Chi Chung [Taïwan] ; Yiu-Kay Lai [Taïwan] ; Margaret Dah-Tsyr Chang [Taïwan] ; Yu-Chen Hu [Taïwan]

Source :

Biotechnology letters [ 0141-5492 ] ; 2005.

RBID : Pascal:05-0390942

Descripteurs français

Pascal (Inist)
- Purification, Protéine, Syndrome, Etude comparative, Chromatographie IMAC, Coronavirus, Baculoviridae, Virus syndrome respiratoire aigu sévère.

English descriptors

KwdEn :
- Baculoviridae, Comparative study, Coronavirus, Immobilized metal affinity chromatography, Protein, Purification, Severe acute respiratory syndrome virus, Syndrome.

Abstract

Histidine-tagged N (rNH) and E (rEH) proteins of Severe Acute Respiratory Syndrome (SARS)-coronovirus were expressed in the baculovirus/insect cell system and purified by immobilized metal affinity chromatography. rNH and rEH proteins differed markedly with respect to expression levels, cell death kinetics and subcellular localizations that led to different extraction and purification schemes. The features of both proteins are compared and the potential applications of purified rNH and rEH are discussed.

Affiliations:

Taïwan

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Pascal:05-0390942

Le document en format XML

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Expression and purification of N and E proteins from Severe Acute Respiratory Syndrome (SARS)-associated coronavirus : a comparative study

Expression and purification of N and E proteins from Severe Acute Respiratory Syndrome (SARS)-associated coronavirus : a comparative study

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Abstract

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