Expression and purification of N and E proteins from Severe Acute Respiratory Syndrome (SARS)-associated coronavirus : a comparative study
Identifieur interne : 000650 ( PascalFrancis/Checkpoint ); précédent : 000649; suivant : 000651Expression and purification of N and E proteins from Severe Acute Respiratory Syndrome (SARS)-associated coronavirus : a comparative study
Auteurs : Chia-Wei Lai [Taïwan] ; Yao-Chi Chung [Taïwan] ; Yiu-Kay Lai [Taïwan] ; Margaret Dah-Tsyr Chang [Taïwan] ; Yu-Chen Hu [Taïwan]Source :
- Biotechnology letters [ 0141-5492 ] ; 2005.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
Histidine-tagged N (rNH) and E (rEH) proteins of Severe Acute Respiratory Syndrome (SARS)-coronovirus were expressed in the baculovirus/insect cell system and purified by immobilized metal affinity chromatography. rNH and rEH proteins differed markedly with respect to expression levels, cell death kinetics and subcellular localizations that led to different extraction and purification schemes. The features of both proteins are compared and the potential applications of purified rNH and rEH are discussed.
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
Pascal:05-0390942Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Expression and purification of N and E proteins from Severe Acute Respiratory Syndrome (SARS)-associated coronavirus : a comparative study</title>
<author><name sortKey="Lai, Chia Wei" sort="Lai, Chia Wei" uniqKey="Lai C" first="Chia-Wei" last="Lai">Chia-Wei Lai</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Chemical Engineering, National Tsing Hua University</s1>
<s2>300, Hsinchu</s2>
<s3>TWN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>Taïwan</country>
<wicri:noRegion>300, Hsinchu</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Institute of Molecular and Cellular Biology, National Tsing Hua University</s1>
<s2>300, Hsinchu</s2>
<s3>TWN</s3>
</inist:fA14>
<country>Taïwan</country>
<wicri:noRegion>300, Hsinchu</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Chung, Yao Chi" sort="Chung, Yao Chi" uniqKey="Chung Y" first="Yao-Chi" last="Chung">Yao-Chi Chung</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Chemical Engineering, National Tsing Hua University</s1>
<s2>300, Hsinchu</s2>
<s3>TWN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>Taïwan</country>
<wicri:noRegion>300, Hsinchu</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lai, Yiu Kay" sort="Lai, Yiu Kay" uniqKey="Lai Y" first="Yiu-Kay" last="Lai">Yiu-Kay Lai</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Institute of Biotechnology, National Tsing Hua University</s1>
<s2>300, Hsinchu</s2>
<s3>TWN</s3>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>Taïwan</country>
<wicri:noRegion>300, Hsinchu</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Chang, Margaret Dah Tsyr" sort="Chang, Margaret Dah Tsyr" uniqKey="Chang M" first="Margaret Dah-Tsyr" last="Chang">Margaret Dah-Tsyr Chang</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Institute of Biotechnology, National Tsing Hua University</s1>
<s2>300, Hsinchu</s2>
<s3>TWN</s3>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>Taïwan</country>
<wicri:noRegion>300, Hsinchu</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hu, Yu Chen" sort="Hu, Yu Chen" uniqKey="Hu Y" first="Yu-Chen" last="Hu">Yu-Chen Hu</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Chemical Engineering, National Tsing Hua University</s1>
<s2>300, Hsinchu</s2>
<s3>TWN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>Taïwan</country>
<wicri:noRegion>300, Hsinchu</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">05-0390942</idno>
<date when="2005">2005</date>
<idno type="stanalyst">PASCAL 05-0390942 INIST</idno>
<idno type="RBID">Pascal:05-0390942</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000620</idno>
<idno type="wicri:Area/PascalFrancis/Curation">000370</idno>
<idno type="wicri:Area/PascalFrancis/Checkpoint">000650</idno>
<idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000650</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Expression and purification of N and E proteins from Severe Acute Respiratory Syndrome (SARS)-associated coronavirus : a comparative study</title>
<author><name sortKey="Lai, Chia Wei" sort="Lai, Chia Wei" uniqKey="Lai C" first="Chia-Wei" last="Lai">Chia-Wei Lai</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Chemical Engineering, National Tsing Hua University</s1>
<s2>300, Hsinchu</s2>
<s3>TWN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>Taïwan</country>
<wicri:noRegion>300, Hsinchu</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Institute of Molecular and Cellular Biology, National Tsing Hua University</s1>
<s2>300, Hsinchu</s2>
<s3>TWN</s3>
</inist:fA14>
<country>Taïwan</country>
<wicri:noRegion>300, Hsinchu</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Chung, Yao Chi" sort="Chung, Yao Chi" uniqKey="Chung Y" first="Yao-Chi" last="Chung">Yao-Chi Chung</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Chemical Engineering, National Tsing Hua University</s1>
<s2>300, Hsinchu</s2>
<s3>TWN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>Taïwan</country>
<wicri:noRegion>300, Hsinchu</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lai, Yiu Kay" sort="Lai, Yiu Kay" uniqKey="Lai Y" first="Yiu-Kay" last="Lai">Yiu-Kay Lai</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Institute of Biotechnology, National Tsing Hua University</s1>
<s2>300, Hsinchu</s2>
<s3>TWN</s3>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>Taïwan</country>
<wicri:noRegion>300, Hsinchu</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Chang, Margaret Dah Tsyr" sort="Chang, Margaret Dah Tsyr" uniqKey="Chang M" first="Margaret Dah-Tsyr" last="Chang">Margaret Dah-Tsyr Chang</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Institute of Biotechnology, National Tsing Hua University</s1>
<s2>300, Hsinchu</s2>
<s3>TWN</s3>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>Taïwan</country>
<wicri:noRegion>300, Hsinchu</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hu, Yu Chen" sort="Hu, Yu Chen" uniqKey="Hu Y" first="Yu-Chen" last="Hu">Yu-Chen Hu</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Chemical Engineering, National Tsing Hua University</s1>
<s2>300, Hsinchu</s2>
<s3>TWN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>Taïwan</country>
<wicri:noRegion>300, Hsinchu</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Biotechnology letters</title>
<title level="j" type="abbreviated">Biotechnol. lett.</title>
<idno type="ISSN">0141-5492</idno>
<imprint><date when="2005">2005</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Biotechnology letters</title>
<title level="j" type="abbreviated">Biotechnol. lett.</title>
<idno type="ISSN">0141-5492</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Baculoviridae</term>
<term>Comparative study</term>
<term>Coronavirus</term>
<term>Immobilized metal affinity chromatography</term>
<term>Protein</term>
<term>Purification</term>
<term>Severe acute respiratory syndrome virus</term>
<term>Syndrome</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Purification</term>
<term>Protéine</term>
<term>Syndrome</term>
<term>Etude comparative</term>
<term>Chromatographie IMAC</term>
<term>Coronavirus</term>
<term>Baculoviridae</term>
<term>Virus syndrome respiratoire aigu sévère</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Histidine-tagged N (rNH) and E (rEH) proteins of Severe Acute Respiratory Syndrome (SARS)-coronovirus were expressed in the baculovirus/insect cell system and purified by immobilized metal affinity chromatography. rNH and rEH proteins differed markedly with respect to expression levels, cell death kinetics and subcellular localizations that led to different extraction and purification schemes. The features of both proteins are compared and the potential applications of purified rNH and rEH are discussed.</div>
</front>
</TEI>
<inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0141-5492</s0>
</fA01>
<fA02 i1="01"><s0>BILED3</s0>
</fA02>
<fA03 i2="1"><s0>Biotechnol. lett.</s0>
</fA03>
<fA05><s2>27</s2>
</fA05>
<fA06><s2>13</s2>
</fA06>
<fA08 i1="01" i2="1" l="ENG"><s1>Expression and purification of N and E proteins from Severe Acute Respiratory Syndrome (SARS)-associated coronavirus : a comparative study</s1>
</fA08>
<fA11 i1="01" i2="1"><s1>LAI (Chia-Wei)</s1>
</fA11>
<fA11 i1="02" i2="1"><s1>CHUNG (Yao-Chi)</s1>
</fA11>
<fA11 i1="03" i2="1"><s1>LAI (Yiu-Kay)</s1>
</fA11>
<fA11 i1="04" i2="1"><s1>CHANG (Margaret Dah-Tsyr)</s1>
</fA11>
<fA11 i1="05" i2="1"><s1>HU (Yu-Chen)</s1>
</fA11>
<fA14 i1="01"><s1>Department of Chemical Engineering, National Tsing Hua University</s1>
<s2>300, Hsinchu</s2>
<s3>TWN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
</fA14>
<fA14 i1="02"><s1>Institute of Biotechnology, National Tsing Hua University</s1>
<s2>300, Hsinchu</s2>
<s3>TWN</s3>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</fA14>
<fA14 i1="03"><s1>Institute of Molecular and Cellular Biology, National Tsing Hua University</s1>
<s2>300, Hsinchu</s2>
<s3>TWN</s3>
</fA14>
<fA20><s1>883-891</s1>
</fA20>
<fA21><s1>2005</s1>
</fA21>
<fA23 i1="01"><s0>ENG</s0>
</fA23>
<fA43 i1="01"><s1>INIST</s1>
<s2>18225</s2>
<s5>354000138473070010</s5>
</fA43>
<fA44><s0>0000</s0>
<s1>© 2005 INIST-CNRS. All rights reserved.</s1>
</fA44>
<fA45><s0>20 ref.</s0>
</fA45>
<fA47 i1="01" i2="1"><s0>05-0390942</s0>
</fA47>
<fA60><s1>P</s1>
</fA60>
<fA61><s0>A</s0>
</fA61>
<fA64 i1="01" i2="1"><s0>Biotechnology letters</s0>
</fA64>
<fA66 i1="01"><s0>NLD</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>Histidine-tagged N (rNH) and E (rEH) proteins of Severe Acute Respiratory Syndrome (SARS)-coronovirus were expressed in the baculovirus/insect cell system and purified by immobilized metal affinity chromatography. rNH and rEH proteins differed markedly with respect to expression levels, cell death kinetics and subcellular localizations that led to different extraction and purification schemes. The features of both proteins are compared and the potential applications of purified rNH and rEH are discussed.</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>002A31</s0>
</fC02>
<fC02 i1="02" i2="X"><s0>215</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Purification</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>Purification</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Purificación</s0>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Protéine</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Protein</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Proteína</s0>
<s5>02</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Syndrome</s0>
<s5>10</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Syndrome</s0>
<s5>10</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Síndrome</s0>
<s5>10</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Etude comparative</s0>
<s5>11</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Comparative study</s0>
<s5>11</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Estudio comparativo</s0>
<s5>11</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Chromatographie IMAC</s0>
<s5>12</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Immobilized metal affinity chromatography</s0>
<s5>12</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Cromatografía IMAC</s0>
<s5>12</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>13</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>13</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Coronavirus</s0>
<s2>NW</s2>
<s5>13</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE"><s0>Baculoviridae</s0>
<s2>NW</s2>
<s5>14</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG"><s0>Baculoviridae</s0>
<s2>NW</s2>
<s5>14</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA"><s0>Baculoviridae</s0>
<s2>NW</s2>
<s5>14</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE"><s0>Virus syndrome respiratoire aigu sévère</s0>
<s2>NW</s2>
<s5>15</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG"><s0>Severe acute respiratory syndrome virus</s0>
<s2>NW</s2>
<s5>15</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA"><s0>Severe acute respiratory syndrome virus</s0>
<s2>NW</s2>
<s5>15</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fN21><s1>276</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
</standard>
</inist>
<affiliations><list><country><li>Taïwan</li>
</country>
</list>
<tree><country name="Taïwan"><noRegion><name sortKey="Lai, Chia Wei" sort="Lai, Chia Wei" uniqKey="Lai C" first="Chia-Wei" last="Lai">Chia-Wei Lai</name>
</noRegion>
<name sortKey="Chang, Margaret Dah Tsyr" sort="Chang, Margaret Dah Tsyr" uniqKey="Chang M" first="Margaret Dah-Tsyr" last="Chang">Margaret Dah-Tsyr Chang</name>
<name sortKey="Chung, Yao Chi" sort="Chung, Yao Chi" uniqKey="Chung Y" first="Yao-Chi" last="Chung">Yao-Chi Chung</name>
<name sortKey="Hu, Yu Chen" sort="Hu, Yu Chen" uniqKey="Hu Y" first="Yu-Chen" last="Hu">Yu-Chen Hu</name>
<name sortKey="Lai, Chia Wei" sort="Lai, Chia Wei" uniqKey="Lai C" first="Chia-Wei" last="Lai">Chia-Wei Lai</name>
<name sortKey="Lai, Yiu Kay" sort="Lai, Yiu Kay" uniqKey="Lai Y" first="Yiu-Kay" last="Lai">Yiu-Kay Lai</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/PascalFrancis/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000650 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PascalFrancis/Checkpoint/biblio.hfd -nk 000650 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= SrasV1 |flux= PascalFrancis |étape= Checkpoint |type= RBID |clé= Pascal:05-0390942 |texte= Expression and purification of N and E proteins from Severe Acute Respiratory Syndrome (SARS)-associated coronavirus : a comparative study }}
![]() | This area was generated with Dilib version V0.6.33. | ![]() |