SrasV1, PascalFrancis, Checkpoint, bibRecord, 000632

Identification of the membrane-active regions of the severe acute respiratory syndrome coronavirus spike membrane glycoprotein using a 16/18-mer peptide scan: Implications for the viral fusion mechanism

Identifieur interne : 000632 ( PascalFrancis/Checkpoint ); précédent : 000631; suivant : 000633

Identification of the membrane-active regions of the severe acute respiratory syndrome coronavirus spike membrane glycoprotein using a 16/18-mer peptide scan: Implications for the viral fusion mechanism

Auteurs : Jaime Guillen [Espagne] ; Ana J. Perez-Berna [Espagne] ; Miguel R. Moreno [Espagne] ; José Villalain [Espagne]

Source :

Journal of virology [ 0022-538X ] ; 2005.

RBID : Pascal:05-0133941

Descripteurs français

Pascal (Inist)
- Coronavirus, Identification, Glycoprotéine, Peptide, Microbiologie, Virologie, Syndrome respiratoire aigu sévère.

English descriptors

KwdEn :
- Coronavirus, Glycoprotein, Identification, Microbiology, Peptides, Severe acute respiratory syndrome, Virology.

Abstract

We have identified the membrane-active regions of the severe acute respiratory syndrome coronavirus (SARS CoV) spike glycoprotein by determining the effect on model membrane integrity of a 16/18-mer SARS CoV spike glycoprotein peptide library. By monitoring the effect of this peptide library on membrane leakage in model membranes, we have identified three regions on the SARS CoV spike glycoprotein with membrane-interacting capabilities: region 1, located immediately upstream of heptad repeat 1 (HR1) and suggested to be the fusion peptide; region 2, located between HR1 and HR2, which would be analogous to the loop domain of human immunodeficiency virus type 1; and region 3, which would correspond to the pretransmembrane region. The identification of these membrane-active regions, which are capable of modifying the biophysical properties of phospholipid membranes, supports their direct role in SARS CoV-mediated membrane fusion, as well as facilitating the future development of SARS CoV entry inhibitors.

Affiliations:

Espagne

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Pascal:05-0133941

Le document en format XML

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Identification of the membrane-active regions of the severe acute respiratory syndrome coronavirus spike membrane glycoprotein using a 16/18-mer peptide scan: Implications for the viral fusion mechanism

Identification of the membrane-active regions of the severe acute respiratory syndrome coronavirus spike membrane glycoprotein using a 16/18-mer peptide scan: Implications for the viral fusion mechanism

Source :

Descripteurs français

English descriptors

Abstract

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Links to Exploration step

Le document en format XML

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