SrasV1, PascalFrancis, Checkpoint, bibRecord, 000592

Rapid and sensitive detection of severe acute respiratory syndrome coronavirus by rolling circle amplification

Identifieur interne : 000592 ( PascalFrancis/Checkpoint ); précédent : 000591; suivant : 000593

Rapid and sensitive detection of severe acute respiratory syndrome coronavirus by rolling circle amplification

Auteurs : BIN WANG [Australie] ; Simon J. Potter [Australie] ; YIGUANG LIN [Australie] ; Anthony L. Cunningham [Australie] ; Dominic E. Dwyer [Australie] ; YUELONG SU [République populaire de Chine] ; XUEJUN MA [République populaire de Chine] ; YUNDE HOU [République populaire de Chine] ; Nitin K. Saksena [Australie]

Source :

Journal of clinical microbiology : (Print) [ 0095-1137 ] ; 2005.

RBID : Pascal:05-0242073

Descripteurs français

Pascal (Inist)
- Coronavirus, Détection, Amplification, Microbiologie, Syndrome respiratoire aigu sévère.

English descriptors

KwdEn :
- Amplification, Coronavirus, Detection, Microbiology, Severe acute respiratory syndrome.

Abstract

The severe acute respiratory syndrome (SARS) epidemic of 2003 was responsible for 774 deaths and caused significant economic damage worldwide. Since July 2003, a number of SARS cases have occurred in China, raising the possibility of future epidemics. We describe here a rapid, sensitive, and highly efficient assay for the detection of SARS coronavirus (SARS-CoV) in cultured material and a small number (n = 7) of clinical samples. Using rolling circle amplification (RCA), we were able to achieve sensitive detection levels of SARS-CoV RNA in both solid and liquid phases. The main advantage of RCA is that it can be performed under isothermal conditions with minimal reagents and avoids the generation of false-positive results, a problem that is frequently encountered in PCR-based assays. Furthermore, the RCA technology provides a faster, more sensitive, and economical option to currently available PCR-based methods.

Affiliations:

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Pascal:05-0242073

Le document en format XML

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<s5>06</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Amplificación</s0>
<s5>06</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Microbiologie</s0>
<s5>07</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Microbiology</s0>
<s5>07</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Microbiología</s0>
<s5>07</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Syndrome respiratoire aigu sévère</s0>
<s2>NM</s2>
<s5>14</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Severe acute respiratory syndrome</s0>
<s2>NM</s2>
<s5>14</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Síndrome respiratorio agudo severo</s0>
<s2>NM</s2>
<s5>14</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Coronaviridae</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Nidovirales</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Virus</s0>
<s2>NW</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Appareil respiratoire pathologie</s0>
<s5>13</s5>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Respiratory disease</s0>
<s5>13</s5>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Aparato respiratorio patología</s0>
<s5>13</s5>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Virose</s0>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Viral disease</s0>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Virosis</s0>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Infection</s0>
</fC07>
<fC07 i1="06" i2="X" l="ENG"><s0>Infection</s0>
</fC07>
<fC07 i1="06" i2="X" l="SPA"><s0>Infección</s0>
</fC07>
<fC07 i1="07" i2="X" l="FRE"><s0>Poumon pathologie</s0>
<s5>16</s5>
</fC07>
<fC07 i1="07" i2="X" l="ENG"><s0>Lung disease</s0>
<s5>16</s5>
</fC07>
<fC07 i1="07" i2="X" l="SPA"><s0>Pulmón patología</s0>
<s5>16</s5>
</fC07>
<fN21><s1>164</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
</standard>
</inist>
<affiliations><list><country><li>Australie</li>
<li>République populaire de Chine</li>
</country>
<region><li>Nouvelle-Galles du Sud</li>
</region>
<settlement><li>Pékin</li>
<li>Sydney</li>
</settlement>
<orgName><li>Université de Sydney</li>
</orgName>
</list>
<tree><country name="Australie"><region name="Nouvelle-Galles du Sud"><name sortKey="Bin Wang" sort="Bin Wang" uniqKey="Bin Wang" last="Bin Wang">BIN WANG</name>
</region>
<name sortKey="Cunningham, Anthony L" sort="Cunningham, Anthony L" uniqKey="Cunningham A" first="Anthony L." last="Cunningham">Anthony L. Cunningham</name>
<name sortKey="Dwyer, Dominic E" sort="Dwyer, Dominic E" uniqKey="Dwyer D" first="Dominic E." last="Dwyer">Dominic E. Dwyer</name>
<name sortKey="Dwyer, Dominic E" sort="Dwyer, Dominic E" uniqKey="Dwyer D" first="Dominic E." last="Dwyer">Dominic E. Dwyer</name>
<name sortKey="Potter, Simon J" sort="Potter, Simon J" uniqKey="Potter S" first="Simon J." last="Potter">Simon J. Potter</name>
<name sortKey="Saksena, Nitin K" sort="Saksena, Nitin K" uniqKey="Saksena N" first="Nitin K." last="Saksena">Nitin K. Saksena</name>
<name sortKey="Yiguang Lin" sort="Yiguang Lin" uniqKey="Yiguang Lin" last="Yiguang Lin">YIGUANG LIN</name>
</country>
<country name="République populaire de Chine"><noRegion><name sortKey="Yuelong Su" sort="Yuelong Su" uniqKey="Yuelong Su" last="Yuelong Su">YUELONG SU</name>
</noRegion>
<name sortKey="Xuejun Ma" sort="Xuejun Ma" uniqKey="Xuejun Ma" last="Xuejun Ma">XUEJUN MA</name>
<name sortKey="Yunde Hou" sort="Yunde Hou" uniqKey="Yunde Hou" last="Yunde Hou">YUNDE HOU</name>
</country>
</tree>
</affiliations>
</record>

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   |texte=   Rapid and sensitive detection of severe acute respiratory syndrome coronavirus by rolling circle amplification
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Rapid and sensitive detection of severe acute respiratory syndrome coronavirus by rolling circle amplification

Rapid and sensitive detection of severe acute respiratory syndrome coronavirus by rolling circle amplification

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