Nasopharyngeal shedding of severe acute respiratory syndrome-associated coronavirus is associated with genetic polymorphisms
Identifieur interne : 000449 ( PascalFrancis/Checkpoint ); précédent : 000448; suivant : 000450Nasopharyngeal shedding of severe acute respiratory syndrome-associated coronavirus is associated with genetic polymorphisms
Auteurs : Wei-Ju Chen [Taïwan] ; Jyh-Yuan Yang [Taïwan] ; Jih-Hui Lin [Taïwan] ; Cathy S. J. Fann [Taïwan] ; Valeriy Osyetrov [Taïwan] ; Chwan-Chuen King [Taïwan] ; Yi-Ming Arthur Chen [Taïwan] ; Hsiao-Ling Chang [Taïwan] ; Hung-Wei Kuo [Taïwan] ; FONG LIAO [Taïwan] ; Mei-Shang Ho [Taïwan]Source :
- Clinical infectious diseases [ 1058-4838 ] ; 2006.
Descripteurs français
- Pascal (Inist)
English descriptors
Abstract
Background. A high initial or peak severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) load in nasopharyngeal specimens was shown to be associated with a high mortality rate. Because all infected individuals were devoid of preeexisting protective immunity against SARS-CoV, the biological basis for the variable virus burdens in different patients remains elusive. Methods. The nationwide SARS database in Taiwan was analyzed, and genotyping of 281 single-nucleotide polymorphisms (SNPs) of 65 genes was performed for 94 patients with SARS, to identify SNPs for which distribution between patients with or without detectable nasopharyngeal shedding of SARS-CoV was biased. Results. Titers of SARS-CoV shed in nasopharyngeal specimens varied widely, ranging from nondetectable to 108 SARS-CoV RNA copies/mL, and they were correlated positively with a high mortality rate (P<.0001, by trend test) and with early death (i.e., death occurring within 2 weeks of the onset of illness) (P =.0015, by trend test). Virus shedding was found to be higher among male patients (P =.0014, by multivariate logistic regression) and among older patients (P =.015, by multivariate logistic regression). Detectable nasopharyngeal shedding of SARS-CoV was associated with polymorphic alleles of interleukins 18 (P =.014) and 1A (P =.031) and a member of NFκB complex (reticuloendotheliosis viral oncogene homolog B [RelB]) (P =.034), all of which are proinflammatory in nature, as well as the procoagulation molecule fibrinogen-like protein 2 (P =.008). Conclusion. The SARS-CoV load is a determinant of clinical outcomes of SARS, and it is associated with polymorphisms of genes involved in innate immunity, which might be regulated in an age- and sex-dependent manner. The findings of the present study provided leads to genes involved in the host response to SARS-CoV infection; if substantiated with functional studies, these findings may be applicable to other newly emerged respiratory viruses (e.g., the influenza pandemic strain).
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
Pascal:06-0348647Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Nasopharyngeal shedding of severe acute respiratory syndrome-associated coronavirus is associated with genetic polymorphisms</title><author><name sortKey="Chen, Wei Ju" sort="Chen, Wei Ju" uniqKey="Chen W" first="Wei-Ju" last="Chen">Wei-Ju Chen</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute of Biomedical Science, Academia Sinica, National Taiwan University</s1><s3>TWN</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Institute of Biomedical Science, Academia Sinica, National Taiwan University</wicri:noRegion></affiliation></author><author><name sortKey="Yang, Jyh Yuan" sort="Yang, Jyh Yuan" uniqKey="Yang J" first="Jyh-Yuan" last="Yang">Jyh-Yuan Yang</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Center for Disease Control, National Taiwan University</s1><s3>TWN</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Center for Disease Control, National Taiwan University</wicri:noRegion></affiliation></author><author><name sortKey="Lin, Jih Hui" sort="Lin, Jih Hui" uniqKey="Lin J" first="Jih-Hui" last="Lin">Jih-Hui Lin</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Center for Disease Control, National Taiwan University</s1><s3>TWN</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Center for Disease Control, National Taiwan University</wicri:noRegion></affiliation></author><author><name sortKey="Fann, Cathy S J" sort="Fann, Cathy S J" uniqKey="Fann C" first="Cathy S. J." last="Fann">Cathy S. J. Fann</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute of Biomedical Science, Academia Sinica, National Taiwan University</s1><s3>TWN</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Institute of Biomedical Science, Academia Sinica, National Taiwan University</wicri:noRegion></affiliation></author><author><name sortKey="Osyetrov, Valeriy" sort="Osyetrov, Valeriy" uniqKey="Osyetrov V" first="Valeriy" last="Osyetrov">Valeriy Osyetrov</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute of Biomedical Science, Academia Sinica, National Taiwan University</s1><s3>TWN</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Institute of Biomedical Science, Academia Sinica, National Taiwan University</wicri:noRegion></affiliation></author><author><name sortKey="King, Chwan Chuen" sort="King, Chwan Chuen" uniqKey="King C" first="Chwan-Chuen" last="King">Chwan-Chuen King</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Graduate Institute of Epidemiology, National Taiwan University</s1><s3>TWN</s3><sZ>6 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Graduate Institute of Epidemiology, National Taiwan University</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Yi Ming Arthur" sort="Chen, Yi Ming Arthur" uniqKey="Chen Y" first="Yi-Ming Arthur" last="Chen">Yi-Ming Arthur Chen</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Institute of Public Health, National Yang-Ming University</s1><s2>Taipei</s2><s3>TWN</s3><sZ>7 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author><author><name sortKey="Chang, Hsiao Ling" sort="Chang, Hsiao Ling" uniqKey="Chang H" first="Hsiao-Ling" last="Chang">Hsiao-Ling Chang</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Center for Disease Control, National Taiwan University</s1><s3>TWN</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Center for Disease Control, National Taiwan University</wicri:noRegion></affiliation></author><author><name sortKey="Kuo, Hung Wei" sort="Kuo, Hung Wei" uniqKey="Kuo H" first="Hung-Wei" last="Kuo">Hung-Wei Kuo</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Center for Disease Control, National Taiwan University</s1><s3>TWN</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Center for Disease Control, National Taiwan University</wicri:noRegion></affiliation></author><author><name sortKey="Fong Liao" sort="Fong Liao" uniqKey="Fong Liao" last="Fong Liao">FONG LIAO</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute of Biomedical Science, Academia Sinica, National Taiwan University</s1><s3>TWN</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Institute of Biomedical Science, Academia Sinica, National Taiwan University</wicri:noRegion></affiliation></author><author><name sortKey="Ho, Mei Shang" sort="Ho, Mei Shang" uniqKey="Ho M" first="Mei-Shang" last="Ho">Mei-Shang Ho</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute of Biomedical Science, Academia Sinica, National Taiwan University</s1><s3>TWN</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Institute of Biomedical Science, Academia Sinica, National Taiwan University</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Graduate Institute of Epidemiology, National Taiwan University</s1><s3>TWN</s3><sZ>6 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Graduate Institute of Epidemiology, National Taiwan University</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Institute of Public Health, National Yang-Ming University</s1><s2>Taipei</s2><s3>TWN</s3><sZ>7 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">06-0348647</idno><date when="2006">2006</date><idno type="stanalyst">PASCAL 06-0348647 INIST</idno><idno type="RBID">Pascal:06-0348647</idno><idno type="wicri:Area/PascalFrancis/Corpus">000479</idno><idno type="wicri:Area/PascalFrancis/Curation">000511</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000449</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000449</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Nasopharyngeal shedding of severe acute respiratory syndrome-associated coronavirus is associated with genetic polymorphisms</title><author><name sortKey="Chen, Wei Ju" sort="Chen, Wei Ju" uniqKey="Chen W" first="Wei-Ju" last="Chen">Wei-Ju Chen</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute of Biomedical Science, Academia Sinica, National Taiwan University</s1><s3>TWN</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Institute of Biomedical Science, Academia Sinica, National Taiwan University</wicri:noRegion></affiliation></author><author><name sortKey="Yang, Jyh Yuan" sort="Yang, Jyh Yuan" uniqKey="Yang J" first="Jyh-Yuan" last="Yang">Jyh-Yuan Yang</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Center for Disease Control, National Taiwan University</s1><s3>TWN</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Center for Disease Control, National Taiwan University</wicri:noRegion></affiliation></author><author><name sortKey="Lin, Jih Hui" sort="Lin, Jih Hui" uniqKey="Lin J" first="Jih-Hui" last="Lin">Jih-Hui Lin</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Center for Disease Control, National Taiwan University</s1><s3>TWN</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Center for Disease Control, National Taiwan University</wicri:noRegion></affiliation></author><author><name sortKey="Fann, Cathy S J" sort="Fann, Cathy S J" uniqKey="Fann C" first="Cathy S. J." last="Fann">Cathy S. J. Fann</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute of Biomedical Science, Academia Sinica, National Taiwan University</s1><s3>TWN</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Institute of Biomedical Science, Academia Sinica, National Taiwan University</wicri:noRegion></affiliation></author><author><name sortKey="Osyetrov, Valeriy" sort="Osyetrov, Valeriy" uniqKey="Osyetrov V" first="Valeriy" last="Osyetrov">Valeriy Osyetrov</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute of Biomedical Science, Academia Sinica, National Taiwan University</s1><s3>TWN</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Institute of Biomedical Science, Academia Sinica, National Taiwan University</wicri:noRegion></affiliation></author><author><name sortKey="King, Chwan Chuen" sort="King, Chwan Chuen" uniqKey="King C" first="Chwan-Chuen" last="King">Chwan-Chuen King</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Graduate Institute of Epidemiology, National Taiwan University</s1><s3>TWN</s3><sZ>6 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Graduate Institute of Epidemiology, National Taiwan University</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Yi Ming Arthur" sort="Chen, Yi Ming Arthur" uniqKey="Chen Y" first="Yi-Ming Arthur" last="Chen">Yi-Ming Arthur Chen</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Institute of Public Health, National Yang-Ming University</s1><s2>Taipei</s2><s3>TWN</s3><sZ>7 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author><author><name sortKey="Chang, Hsiao Ling" sort="Chang, Hsiao Ling" uniqKey="Chang H" first="Hsiao-Ling" last="Chang">Hsiao-Ling Chang</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Center for Disease Control, National Taiwan University</s1><s3>TWN</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Center for Disease Control, National Taiwan University</wicri:noRegion></affiliation></author><author><name sortKey="Kuo, Hung Wei" sort="Kuo, Hung Wei" uniqKey="Kuo H" first="Hung-Wei" last="Kuo">Hung-Wei Kuo</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Center for Disease Control, National Taiwan University</s1><s3>TWN</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Center for Disease Control, National Taiwan University</wicri:noRegion></affiliation></author><author><name sortKey="Fong Liao" sort="Fong Liao" uniqKey="Fong Liao" last="Fong Liao">FONG LIAO</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute of Biomedical Science, Academia Sinica, National Taiwan University</s1><s3>TWN</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Institute of Biomedical Science, Academia Sinica, National Taiwan University</wicri:noRegion></affiliation></author><author><name sortKey="Ho, Mei Shang" sort="Ho, Mei Shang" uniqKey="Ho M" first="Mei-Shang" last="Ho">Mei-Shang Ho</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute of Biomedical Science, Academia Sinica, National Taiwan University</s1><s3>TWN</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Institute of Biomedical Science, Academia Sinica, National Taiwan University</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Graduate Institute of Epidemiology, National Taiwan University</s1><s3>TWN</s3><sZ>6 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Graduate Institute of Epidemiology, National Taiwan University</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Institute of Public Health, National Yang-Ming University</s1><s2>Taipei</s2><s3>TWN</s3><sZ>7 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Clinical infectious diseases</title><title level="j" type="abbreviated">Clin. infect. dis.</title><idno type="ISSN">1058-4838</idno><imprint><date when="2006">2006</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Clinical infectious diseases</title><title level="j" type="abbreviated">Clin. infect. dis.</title><idno type="ISSN">1058-4838</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Coronavirus</term><term>Nasopharynx</term><term>Polymorphism</term><term>Severe acute respiratory syndrome</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term><term>Nasopharynx</term><term>Polymorphisme</term><term>Coronavirus</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Background. A high initial or peak severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) load in nasopharyngeal specimens was shown to be associated with a high mortality rate. Because all infected individuals were devoid of preeexisting protective immunity against SARS-CoV, the biological basis for the variable virus burdens in different patients remains elusive. Methods. The nationwide SARS database in Taiwan was analyzed, and genotyping of 281 single-nucleotide polymorphisms (SNPs) of 65 genes was performed for 94 patients with SARS, to identify SNPs for which distribution between patients with or without detectable nasopharyngeal shedding of SARS-CoV was biased. Results. Titers of SARS-CoV shed in nasopharyngeal specimens varied widely, ranging from nondetectable to 10<sup>8</sup> SARS-CoV RNA copies/mL, and they were correlated positively with a high mortality rate (P<.0001, by trend test) and with early death (i.e., death occurring within 2 weeks of the onset of illness) (P =.0015, by trend test). Virus shedding was found to be higher among male patients (P =.0014, by multivariate logistic regression) and among older patients (P =.015, by multivariate logistic regression). Detectable nasopharyngeal shedding of SARS-CoV was associated with polymorphic alleles of interleukins 18 (P =.014) and 1A (P =.031) and a member of NFκB complex (reticuloendotheliosis viral oncogene homolog B [RelB]) (P =.034), all of which are proinflammatory in nature, as well as the procoagulation molecule fibrinogen-like protein 2 (P =.008). Conclusion. The SARS-CoV load is a determinant of clinical outcomes of SARS, and it is associated with polymorphisms of genes involved in innate immunity, which might be regulated in an age- and sex-dependent manner. The findings of the present study provided leads to genes involved in the host response to SARS-CoV infection; if substantiated with functional studies, these findings may be applicable to other newly emerged respiratory viruses (e.g., the influenza pandemic strain).</div></front></TEI><inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>1058-4838</s0></fA01><fA02 i1="01"><s0>CIDIEL</s0></fA02><fA03 i2="1"><s0>Clin. infect. dis.</s0></fA03><fA05><s2>42</s2></fA05><fA06><s2>11</s2></fA06><fA08 i1="01" i2="1" l="ENG"><s1>Nasopharyngeal shedding of severe acute respiratory syndrome-associated coronavirus is associated with genetic polymorphisms</s1></fA08><fA11 i1="01" i2="1"><s1>CHEN (Wei-Ju)</s1></fA11><fA11 i1="02" i2="1"><s1>YANG (Jyh-Yuan)</s1></fA11><fA11 i1="03" i2="1"><s1>LIN (Jih-Hui)</s1></fA11><fA11 i1="04" i2="1"><s1>FANN (Cathy S. J.)</s1></fA11><fA11 i1="05" i2="1"><s1>OSYETROV (Valeriy)</s1></fA11><fA11 i1="06" i2="1"><s1>KING (Chwan-Chuen)</s1></fA11><fA11 i1="07" i2="1"><s1>CHEN (Yi-Ming Arthur)</s1></fA11><fA11 i1="08" i2="1"><s1>CHANG (Hsiao-Ling)</s1></fA11><fA11 i1="09" i2="1"><s1>KUO (Hung-Wei)</s1></fA11><fA11 i1="10" i2="1"><s1>FONG LIAO</s1></fA11><fA11 i1="11" i2="1"><s1>HO (Mei-Shang)</s1></fA11><fA14 i1="01"><s1>Institute of Biomedical Science, Academia Sinica, National Taiwan University</s1><s3>TWN</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></fA14><fA14 i1="02"><s1>Center for Disease Control, National Taiwan University</s1><s3>TWN</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></fA14><fA14 i1="03"><s1>Graduate Institute of Epidemiology, National Taiwan University</s1><s3>TWN</s3><sZ>6 aut.</sZ><sZ>11 aut.</sZ></fA14><fA14 i1="04"><s1>Institute of Public Health, National Yang-Ming University</s1><s2>Taipei</s2><s3>TWN</s3><sZ>7 aut.</sZ><sZ>11 aut.</sZ></fA14><fA20><s1>1561-1569</s1></fA20><fA21><s1>2006</s1></fA21><fA23 i1="01"><s0>ENG</s0></fA23><fA43 i1="01"><s1>INIST</s1><s2>18407</s2><s5>354000142370600070</s5></fA43><fA44><s0>0000</s0><s1>© 2006 INIST-CNRS. All rights reserved.</s1></fA44><fA45><s0>46 ref.</s0></fA45><fA47 i1="01" i2="1"><s0>06-0348647</s0></fA47><fA60><s1>P</s1></fA60><fA61><s0>A</s0></fA61><fA64 i1="01" i2="1"><s0>Clinical infectious diseases</s0></fA64><fA66 i1="01"><s0>USA</s0></fA66><fC01 i1="01" l="ENG"><s0>Background. A high initial or peak severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) load in nasopharyngeal specimens was shown to be associated with a high mortality rate. Because all infected individuals were devoid of preeexisting protective immunity against SARS-CoV, the biological basis for the variable virus burdens in different patients remains elusive. Methods. The nationwide SARS database in Taiwan was analyzed, and genotyping of 281 single-nucleotide polymorphisms (SNPs) of 65 genes was performed for 94 patients with SARS, to identify SNPs for which distribution between patients with or without detectable nasopharyngeal shedding of SARS-CoV was biased. Results. Titers of SARS-CoV shed in nasopharyngeal specimens varied widely, ranging from nondetectable to 10<sup>8</sup> SARS-CoV RNA copies/mL, and they were correlated positively with a high mortality rate (P<.0001, by trend test) and with early death (i.e., death occurring within 2 weeks of the onset of illness) (P =.0015, by trend test). Virus shedding was found to be higher among male patients (P =.0014, by multivariate logistic regression) and among older patients (P =.015, by multivariate logistic regression). Detectable nasopharyngeal shedding of SARS-CoV was associated with polymorphic alleles of interleukins 18 (P =.014) and 1A (P =.031) and a member of NFκB complex (reticuloendotheliosis viral oncogene homolog B [RelB]) (P =.034), all of which are proinflammatory in nature, as well as the procoagulation molecule fibrinogen-like protein 2 (P =.008). Conclusion. The SARS-CoV load is a determinant of clinical outcomes of SARS, and it is associated with polymorphisms of genes involved in innate immunity, which might be regulated in an age- and sex-dependent manner. The findings of the present study provided leads to genes involved in the host response to SARS-CoV infection; if substantiated with functional studies, these findings may be applicable to other newly emerged respiratory viruses (e.g., the influenza pandemic strain).</s0></fC01><fC02 i1="01" i2="X"><s0>002B05C02C</s0></fC02><fC03 i1="01" i2="X" l="FRE"><s0>Syndrome respiratoire aigu sévère</s0><s2>NM</s2><s5>01</s5></fC03><fC03 i1="01" i2="X" l="ENG"><s0>Severe acute respiratory syndrome</s0><s2>NM</s2><s5>01</s5></fC03><fC03 i1="01" i2="X" l="SPA"><s0>Síndrome respiratorio agudo severo</s0><s2>NM</s2><s5>01</s5></fC03><fC03 i1="02" i2="X" l="FRE"><s0>Nasopharynx</s0><s5>07</s5></fC03><fC03 i1="02" i2="X" l="ENG"><s0>Nasopharynx</s0><s5>07</s5></fC03><fC03 i1="02" i2="X" l="SPA"><s0>Nasofaringe</s0><s5>07</s5></fC03><fC03 i1="03" i2="X" l="FRE"><s0>Polymorphisme</s0><s5>08</s5></fC03><fC03 i1="03" i2="X" l="ENG"><s0>Polymorphism</s0><s5>08</s5></fC03><fC03 i1="03" i2="X" l="SPA"><s0>Polimorfismo</s0><s5>08</s5></fC03><fC03 i1="04" i2="X" l="FRE"><s0>Coronavirus</s0><s2>NW</s2><s5>10</s5></fC03><fC03 i1="04" i2="X" l="ENG"><s0>Coronavirus</s0><s2>NW</s2><s5>10</s5></fC03><fC03 i1="04" i2="X" l="SPA"><s0>Coronavirus</s0><s2>NW</s2><s5>10</s5></fC03><fC07 i1="01" i2="X" l="FRE"><s0>Virose</s0></fC07><fC07 i1="01" i2="X" l="ENG"><s0>Viral disease</s0></fC07><fC07 i1="01" i2="X" l="SPA"><s0>Virosis</s0></fC07><fC07 i1="02" i2="X" l="FRE"><s0>Infection</s0></fC07><fC07 i1="02" i2="X" l="ENG"><s0>Infection</s0></fC07><fC07 i1="02" i2="X" l="SPA"><s0>Infección</s0></fC07><fC07 i1="03" i2="X" l="FRE"><s0>Coronaviridae</s0><s2>NW</s2></fC07><fC07 i1="03" i2="X" l="ENG"><s0>Coronaviridae</s0><s2>NW</s2></fC07><fC07 i1="03" i2="X" l="SPA"><s0>Coronaviridae</s0><s2>NW</s2></fC07><fC07 i1="04" i2="X" l="FRE"><s0>Nidovirales</s0><s2>NW</s2></fC07><fC07 i1="04" i2="X" l="ENG"><s0>Nidovirales</s0><s2>NW</s2></fC07><fC07 i1="04" i2="X" l="SPA"><s0>Nidovirales</s0><s2>NW</s2></fC07><fC07 i1="05" i2="X" l="FRE"><s0>Virus</s0><s2>NW</s2></fC07><fC07 i1="05" i2="X" l="ENG"><s0>Virus</s0><s2>NW</s2></fC07><fC07 i1="05" i2="X" l="SPA"><s0>Virus</s0><s2>NW</s2></fC07><fC07 i1="06" i2="X" l="FRE"><s0>Appareil respiratoire pathologie</s0><s5>37</s5></fC07><fC07 i1="06" i2="X" l="ENG"><s0>Respiratory disease</s0><s5>37</s5></fC07><fC07 i1="06" i2="X" l="SPA"><s0>Aparato respiratorio patología</s0><s5>37</s5></fC07><fC07 i1="07" i2="X" l="FRE"><s0>Poumon pathologie</s0><s5>38</s5></fC07><fC07 i1="07" i2="X" l="ENG"><s0>Lung disease</s0><s5>38</s5></fC07><fC07 i1="07" i2="X" l="SPA"><s0>Pulmón patología</s0><s5>38</s5></fC07><fN21><s1>227</s1></fN21><fN44 i1="01"><s1>OTO</s1></fN44><fN82><s1>OTO</s1></fN82></pA></standard></inist><affiliations><list><country><li>Taïwan</li></country></list><tree><country name="Taïwan"><noRegion><name sortKey="Chen, Wei Ju" sort="Chen, Wei Ju" uniqKey="Chen W" first="Wei-Ju" last="Chen">Wei-Ju Chen</name></noRegion><name sortKey="Chang, Hsiao Ling" sort="Chang, Hsiao Ling" uniqKey="Chang H" first="Hsiao-Ling" last="Chang">Hsiao-Ling Chang</name><name sortKey="Chen, Yi Ming Arthur" sort="Chen, Yi Ming Arthur" uniqKey="Chen Y" first="Yi-Ming Arthur" last="Chen">Yi-Ming Arthur Chen</name><name sortKey="Fann, Cathy S J" sort="Fann, Cathy S J" uniqKey="Fann C" first="Cathy S. J." last="Fann">Cathy S. J. Fann</name><name sortKey="Fong Liao" sort="Fong Liao" uniqKey="Fong Liao" last="Fong Liao">FONG LIAO</name><name sortKey="Ho, Mei Shang" sort="Ho, Mei Shang" uniqKey="Ho M" first="Mei-Shang" last="Ho">Mei-Shang Ho</name><name sortKey="Ho, Mei Shang" sort="Ho, Mei Shang" uniqKey="Ho M" first="Mei-Shang" last="Ho">Mei-Shang Ho</name><name sortKey="Ho, Mei Shang" sort="Ho, Mei Shang" uniqKey="Ho M" first="Mei-Shang" last="Ho">Mei-Shang Ho</name><name sortKey="King, Chwan Chuen" sort="King, Chwan Chuen" uniqKey="King C" first="Chwan-Chuen" last="King">Chwan-Chuen King</name><name sortKey="Kuo, Hung Wei" sort="Kuo, Hung Wei" uniqKey="Kuo H" first="Hung-Wei" last="Kuo">Hung-Wei Kuo</name><name sortKey="Lin, Jih Hui" sort="Lin, Jih Hui" uniqKey="Lin J" first="Jih-Hui" last="Lin">Jih-Hui Lin</name><name sortKey="Osyetrov, Valeriy" sort="Osyetrov, Valeriy" uniqKey="Osyetrov V" first="Valeriy" last="Osyetrov">Valeriy Osyetrov</name><name sortKey="Yang, Jyh Yuan" sort="Yang, Jyh Yuan" uniqKey="Yang J" first="Jyh-Yuan" last="Yang">Jyh-Yuan Yang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/PascalFrancis/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000449 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PascalFrancis/Checkpoint/biblio.hfd -nk 000449 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= PascalFrancis
|étape= Checkpoint
|type= RBID
|clé= Pascal:06-0348647
|texte= Nasopharyngeal shedding of severe acute respiratory syndrome-associated coronavirus is associated with genetic polymorphisms
}}
| This area was generated with Dilib version V0.6.33. |  |