Amino acid substitutions in the S2 region enhance severe acute respiratory syndrome coronavirus infectivity in rat angiotensin-converting enzyme 2-expressing cells
Identifieur interne : 000401 ( PascalFrancis/Checkpoint ); précédent : 000400; suivant : 000402Amino acid substitutions in the S2 region enhance severe acute respiratory syndrome coronavirus infectivity in rat angiotensin-converting enzyme 2-expressing cells
Auteurs : Shuetsu Fukushi [Japon] ; Tetsuya Mizutani [Japon] ; Kouji Sakai [Japon] ; Masayuki Saijo [Japon] ; Fumihiro Taguchi [Japon] ; Masaru Yokoyama [Japon] ; Ichiro Kurane [Japon] ; Shigeru Morikawa [Japon]Source :
- Journal of virology [ 0022-538X ] ; 2007.
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Abstract
To clarify the molecular basis of severe acute respiratory syndrome coronavirus (SARS-CoV) adaptation to different host species, we serially passaged SARS-CoV in rat angiotensin-converting enzyme 2 (ACE2)-express-ing cells. After 15 passages, the virus (Rat-P15) came to replicate effectively in rat ACE2-expressing cells. Two amino acid substitutions in the S2 region were found on the Rat-P15 S gene. Analyses of the infectivity of the pseudotype-bearing S protein indicated that the two substitutions in the S2 region, especially the S950F substitution, were responsible for efficient infection. Therefore, virus adaptation to different host species can be induced by amino acid substitutions in the S2 region.
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wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology I, National Institute of Infectious Diseases, Gakuen 4-7-1</s1><s2>Musashimurayama, Tokyo 208-0011</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Sakai, Kouji" sort="Sakai, Kouji" uniqKey="Sakai K" first="Kouji" last="Sakai">Kouji Sakai</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology I, National Institute of Infectious Diseases, Gakuen 4-7-1</s1><s2>Musashimurayama, Tokyo 208-0011</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de 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aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Yokoyama, Masaru" sort="Yokoyama, Masaru" uniqKey="Yokoyama M" first="Masaru" last="Yokoyama">Masaru Yokoyama</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Center for Pathogen Genomics, National Institute of Infectious Diseases, Gakuen 4-7-1</s1><s2>Musashimurayama, Tokyo 208-0011</s2><s3>JPN</s3><sZ>6 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Kurane, Ichiro" sort="Kurane, Ichiro" uniqKey="Kurane I" first="Ichiro" last="Kurane">Ichiro Kurane</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology I, National Institute of Infectious Diseases, Gakuen 4-7-1</s1><s2>Musashimurayama, Tokyo 208-0011</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Morikawa, Shigeru" sort="Morikawa, Shigeru" uniqKey="Morikawa S" first="Shigeru" last="Morikawa">Shigeru Morikawa</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology I, National Institute of Infectious Diseases, Gakuen 4-7-1</s1><s2>Musashimurayama, Tokyo 208-0011</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">07-0486461</idno><date when="2007">2007</date><idno type="stanalyst">PASCAL 07-0486461 INIST</idno><idno type="RBID">Pascal:07-0486461</idno><idno type="wicri:Area/PascalFrancis/Corpus">000335</idno><idno type="wicri:Area/PascalFrancis/Curation">000654</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000401</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000401</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Amino acid substitutions in the S2 region enhance severe acute respiratory syndrome coronavirus infectivity in rat angiotensin-converting enzyme 2-expressing cells</title><author><name sortKey="Fukushi, Shuetsu" sort="Fukushi, Shuetsu" uniqKey="Fukushi S" first="Shuetsu" last="Fukushi">Shuetsu Fukushi</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology I, National Institute of Infectious Diseases, Gakuen 4-7-1</s1><s2>Musashimurayama, Tokyo 208-0011</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Mizutani, Tetsuya" sort="Mizutani, Tetsuya" uniqKey="Mizutani T" first="Tetsuya" last="Mizutani">Tetsuya Mizutani</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology I, National Institute of Infectious Diseases, Gakuen 4-7-1</s1><s2>Musashimurayama, Tokyo 208-0011</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Sakai, Kouji" sort="Sakai, Kouji" uniqKey="Sakai K" first="Kouji" last="Sakai">Kouji Sakai</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology I, National Institute of Infectious Diseases, Gakuen 4-7-1</s1><s2>Musashimurayama, Tokyo 208-0011</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Saijo, Masayuki" sort="Saijo, Masayuki" uniqKey="Saijo M" first="Masayuki" last="Saijo">Masayuki Saijo</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology I, National Institute of Infectious Diseases, Gakuen 4-7-1</s1><s2>Musashimurayama, Tokyo 208-0011</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Taguchi, Fumihiro" sort="Taguchi, Fumihiro" uniqKey="Taguchi F" first="Fumihiro" last="Taguchi">Fumihiro Taguchi</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Virology III, National Institute of Infectious Diseases, Gakuen 4-7-1</s1><s2>Musashimurayama, Tokyo 208-0011</s2><s3>JPN</s3><sZ>5 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Yokoyama, Masaru" sort="Yokoyama, Masaru" uniqKey="Yokoyama M" first="Masaru" last="Yokoyama">Masaru Yokoyama</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Center for Pathogen Genomics, National Institute of Infectious Diseases, Gakuen 4-7-1</s1><s2>Musashimurayama, Tokyo 208-0011</s2><s3>JPN</s3><sZ>6 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Kurane, Ichiro" sort="Kurane, Ichiro" uniqKey="Kurane I" first="Ichiro" last="Kurane">Ichiro Kurane</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology I, National Institute of Infectious Diseases, Gakuen 4-7-1</s1><s2>Musashimurayama, Tokyo 208-0011</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Morikawa, Shigeru" sort="Morikawa, Shigeru" uniqKey="Morikawa S" first="Shigeru" last="Morikawa">Shigeru Morikawa</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology I, National Institute of Infectious Diseases, Gakuen 4-7-1</s1><s2>Musashimurayama, Tokyo 208-0011</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author></analytic><series><title level="j" type="main">Journal of virology</title><title level="j" type="abbreviated">J. virol.</title><idno type="ISSN">0022-538X</idno><imprint><date when="2007">2007</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of virology</title><title level="j" type="abbreviated">J. virol.</title><idno type="ISSN">0022-538X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acute</term><term>In vitro</term><term>Infectivity</term><term>Rat coronavirus</term><term>Virology</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Coronavirus rat</term><term>Aigu</term><term>Pouvoir infectant</term><term>In vitro</term><term>Virologie</term><term>Angiotensin converting enzyme 2</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To clarify the molecular basis of severe acute respiratory syndrome coronavirus (SARS-CoV) adaptation to different host species, we serially passaged SARS-CoV in rat angiotensin-converting enzyme 2 (ACE2)-express-ing cells. After 15 passages, the virus (Rat-P15) came to replicate effectively in rat ACE2-expressing cells. Two amino acid substitutions in the S2 region were found on the Rat-P15 S gene. Analyses of the infectivity of the pseudotype-bearing S protein indicated that the two substitutions in the S2 region, especially the S950F substitution, were responsible for efficient infection. Therefore, virus adaptation to different host species can be induced by amino acid substitutions in the S2 region.</div></front></TEI><inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0022-538X</s0></fA01><fA03 i2="1"><s0>J. virol.</s0></fA03><fA05><s2>81</s2></fA05><fA06><s2>19</s2></fA06><fA08 i1="01" i2="1" l="ENG"><s1>Amino acid substitutions in the S2 region enhance severe acute respiratory syndrome coronavirus infectivity in rat angiotensin-converting enzyme 2-expressing cells</s1></fA08><fA11 i1="01" i2="1"><s1>FUKUSHI (Shuetsu)</s1></fA11><fA11 i1="02" i2="1"><s1>MIZUTANI (Tetsuya)</s1></fA11><fA11 i1="03" i2="1"><s1>SAKAI (Kouji)</s1></fA11><fA11 i1="04" i2="1"><s1>SAIJO (Masayuki)</s1></fA11><fA11 i1="05" i2="1"><s1>TAGUCHI (Fumihiro)</s1></fA11><fA11 i1="06" i2="1"><s1>YOKOYAMA (Masaru)</s1></fA11><fA11 i1="07" i2="1"><s1>KURANE (Ichiro)</s1></fA11><fA11 i1="08" i2="1"><s1>MORIKAWA (Shigeru)</s1></fA11><fA14 i1="01"><s1>Department of Virology I, National Institute of Infectious Diseases, Gakuen 4-7-1</s1><s2>Musashimurayama, Tokyo 208-0011</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></fA14><fA14 i1="02"><s1>Department of Virology III, National Institute of Infectious Diseases, Gakuen 4-7-1</s1><s2>Musashimurayama, Tokyo 208-0011</s2><s3>JPN</s3><sZ>5 aut.</sZ></fA14><fA14 i1="03"><s1>Center for Pathogen Genomics, National Institute of Infectious Diseases, Gakuen 4-7-1</s1><s2>Musashimurayama, Tokyo 208-0011</s2><s3>JPN</s3><sZ>6 aut.</sZ></fA14><fA20><s1>10831-10834</s1></fA20><fA21><s1>2007</s1></fA21><fA23 i1="01"><s0>ENG</s0></fA23><fA43 i1="01"><s1>INIST</s1><s2>13592</s2><s5>354000160854590620</s5></fA43><fA44><s0>0000</s0><s1>© 2007 INIST-CNRS. All rights reserved.</s1></fA44><fA45><s0>29 ref.</s0></fA45><fA47 i1="01" i2="1"><s0>07-0486461</s0></fA47><fA60><s1>P</s1></fA60><fA61><s0>A</s0></fA61><fA64 i1="01" i2="1"><s0>Journal of virology</s0></fA64><fA66 i1="01"><s0>USA</s0></fA66><fC01 i1="01" l="ENG"><s0>To clarify the molecular basis of severe acute respiratory syndrome coronavirus (SARS-CoV) adaptation to different host species, we serially passaged SARS-CoV in rat angiotensin-converting enzyme 2 (ACE2)-express-ing cells. After 15 passages, the virus (Rat-P15) came to replicate effectively in rat ACE2-expressing cells. Two amino acid substitutions in the S2 region were found on the Rat-P15 S gene. Analyses of the infectivity of the pseudotype-bearing S protein indicated that the two substitutions in the S2 region, especially the S950F substitution, were responsible for efficient infection. Therefore, virus adaptation to different host species can be induced by amino acid substitutions in the S2 region.</s0></fC01><fC02 i1="01" i2="X"><s0>002A05C10</s0></fC02><fC03 i1="01" i2="X" l="FRE"><s0>Coronavirus rat</s0><s2>NW</s2><s5>01</s5></fC03><fC03 i1="01" i2="X" l="ENG"><s0>Rat coronavirus</s0><s2>NW</s2><s5>01</s5></fC03><fC03 i1="01" i2="X" l="SPA"><s0>Rat coronavirus</s0><s2>NW</s2><s5>01</s5></fC03><fC03 i1="02" i2="X" l="FRE"><s0>Aigu</s0><s5>05</s5></fC03><fC03 i1="02" i2="X" l="ENG"><s0>Acute</s0><s5>05</s5></fC03><fC03 i1="02" i2="X" l="SPA"><s0>Agudo</s0><s5>05</s5></fC03><fC03 i1="03" i2="X" l="FRE"><s0>Pouvoir infectant</s0><s5>06</s5></fC03><fC03 i1="03" i2="X" l="ENG"><s0>Infectivity</s0><s5>06</s5></fC03><fC03 i1="03" i2="X" l="SPA"><s0>Poder infectante</s0><s5>06</s5></fC03><fC03 i1="04" i2="X" l="FRE"><s0>In vitro</s0><s5>07</s5></fC03><fC03 i1="04" i2="X" l="ENG"><s0>In vitro</s0><s5>07</s5></fC03><fC03 i1="04" i2="X" l="SPA"><s0>In vitro</s0><s5>07</s5></fC03><fC03 i1="05" i2="X" l="FRE"><s0>Virologie</s0><s5>08</s5></fC03><fC03 i1="05" i2="X" l="ENG"><s0>Virology</s0><s5>08</s5></fC03><fC03 i1="05" i2="X" l="SPA"><s0>Virología</s0><s5>08</s5></fC03><fC03 i1="06" i2="X" l="FRE"><s0>Angiotensin converting enzyme 2</s0><s4>INC</s4><s5>79</s5></fC03><fC07 i1="01" i2="X" l="FRE"><s0>Coronavirus</s0><s2>NW</s2></fC07><fC07 i1="01" i2="X" l="ENG"><s0>Coronavirus</s0><s2>NW</s2></fC07><fC07 i1="01" i2="X" l="SPA"><s0>Coronavirus</s0><s2>NW</s2></fC07><fC07 i1="02" i2="X" l="FRE"><s0>Coronaviridae</s0><s2>NW</s2></fC07><fC07 i1="02" i2="X" l="ENG"><s0>Coronaviridae</s0><s2>NW</s2></fC07><fC07 i1="02" i2="X" l="SPA"><s0>Coronaviridae</s0><s2>NW</s2></fC07><fC07 i1="03" i2="X" l="FRE"><s0>Nidovirales</s0><s2>NW</s2></fC07><fC07 i1="03" i2="X" l="ENG"><s0>Nidovirales</s0><s2>NW</s2></fC07><fC07 i1="03" i2="X" l="SPA"><s0>Nidovirales</s0><s2>NW</s2></fC07><fC07 i1="04" i2="X" l="FRE"><s0>Virus</s0><s2>NW</s2></fC07><fC07 i1="04" i2="X" l="ENG"><s0>Virus</s0><s2>NW</s2></fC07><fC07 i1="04" i2="X" l="SPA"><s0>Virus</s0><s2>NW</s2></fC07><fN21><s1>316</s1></fN21><fN44 i1="01"><s1>OTO</s1></fN44><fN82><s1>OTO</s1></fN82></pA></standard></inist><affiliations><list><country><li>Japon</li></country><region><li>Région de Kantō</li></region><settlement><li>Tokyo</li></settlement></list><tree><country name="Japon"><region name="Région de Kantō"><name sortKey="Fukushi, Shuetsu" sort="Fukushi, Shuetsu" uniqKey="Fukushi S" first="Shuetsu" last="Fukushi">Shuetsu Fukushi</name></region><name sortKey="Kurane, Ichiro" sort="Kurane, Ichiro" uniqKey="Kurane I" first="Ichiro" last="Kurane">Ichiro Kurane</name><name sortKey="Mizutani, Tetsuya" sort="Mizutani, Tetsuya" uniqKey="Mizutani T" first="Tetsuya" last="Mizutani">Tetsuya Mizutani</name><name sortKey="Morikawa, Shigeru" sort="Morikawa, Shigeru" uniqKey="Morikawa S" first="Shigeru" last="Morikawa">Shigeru Morikawa</name><name sortKey="Saijo, Masayuki" sort="Saijo, Masayuki" uniqKey="Saijo M" first="Masayuki" last="Saijo">Masayuki Saijo</name><name sortKey="Sakai, Kouji" sort="Sakai, Kouji" uniqKey="Sakai K" first="Kouji" last="Sakai">Kouji Sakai</name><name sortKey="Taguchi, Fumihiro" sort="Taguchi, Fumihiro" uniqKey="Taguchi F" first="Fumihiro" last="Taguchi">Fumihiro Taguchi</name><name sortKey="Yokoyama, Masaru" sort="Yokoyama, Masaru" uniqKey="Yokoyama M" first="Masaru" last="Yokoyama">Masaru Yokoyama</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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