A rapid point of care immunoswab assay for SARS-CoV detection
Identifieur interne : 000300 ( PascalFrancis/Checkpoint ); précédent : 000299; suivant : 000301A rapid point of care immunoswab assay for SARS-CoV detection
Auteurs : Sriram Kammila [Canada] ; Dipankar Das [Canada] ; Pravin K. Bhatnagar [Canada] ; Hoon H. Sunwoo [Canada] ; Gustavo Zayas-Zamora [Canada] ; Malcolm King [Canada] ; Mavanur R. Suresh [Canada]Source :
- Journal of virological methods [ 0166-0934 ] ; 2008.
Descripteurs français
- Pascal (Inist)
English descriptors
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Abstract
The emergence of severe acute respiratory syndrome (SARS) resulted in several outbreaks worldwide. Early tests for diagnosis were not always conclusive in identifying a SARS suspected patient Nucleocapsid protein (NP) is the most predominant virus derived structural protein which is shed in high amounts in serum and nasopharyngeal aspirate during the first week of infection. As part of such efforts, a simple, easy to use immunoswab method was developed by generating a panel of monoclonal antibodies (MAbs), Bispecific MAbs and chicken polyclonal IgY antibody against the SARS-CoV nucleocapsid protein (NP). Employing the MAb-based immunoswab, an NP concentration of 200 pg/mL in saline and pig nasopharyngeal aspirate, and 500 pg/mL in rabbit serum were detected. BsMAb-based immunoswabs detected an NP concentration of 20 pg/mL in saline, 500 pg/mL in rabbit serum and 20-200 pg/mL in pig nasopharyngeal aspirate. Polyclonal IgY-based immunoswabs detected an NP concentration of 10 pg/mL in pig nasopharyngeal aspirate providing the most sensitive SARS point of care assay. Results show that the robust immunoswab method of detecting SARS-CoV NP antigen can be developed into an easy and effective way of identifying SARS suspected individuals during a future SARS epidemic, thereby reducing and containing the transmission. The key feature of this simple immunoswab diagnostic assay is its ability to detect the presence of the SARS-CoV antigen within 45-60 min with the availability of the body fluid samples.
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Sunwoo</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, 11304-89 Avenue</s1><s2>Edmonton, Alberta, T6G 2N8</s2><s3>CAN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Edmonton, Alberta, T6G 2N8</wicri:noRegion></affiliation></author><author><name sortKey="Zayas Zamora, Gustavo" sort="Zayas Zamora, Gustavo" uniqKey="Zayas Zamora G" first="Gustavo" last="Zayas-Zamora">Gustavo Zayas-Zamora</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Mucophysiology Laboratory, 173 Heritage Medical Research Centre, Department of Medicine, Faculty of Medicine & Dentistry, University of Alberta</s1><s2>Alberta, T6G 2S2</s2><s3>CAN</s3><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Alberta, T6G 2S2</wicri:noRegion></affiliation></author><author><name sortKey="King, Malcolm" sort="King, Malcolm" uniqKey="King M" first="Malcolm" last="King">Malcolm King</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Mucophysiology Laboratory, 173 Heritage Medical Research Centre, Department of Medicine, Faculty of Medicine & Dentistry, University of Alberta</s1><s2>Alberta, T6G 2S2</s2><s3>CAN</s3><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Alberta, T6G 2S2</wicri:noRegion></affiliation></author><author><name sortKey="Suresh, Mavanur R" sort="Suresh, Mavanur R" uniqKey="Suresh M" first="Mavanur R." last="Suresh">Mavanur R. Suresh</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, 11304-89 Avenue</s1><s2>Edmonton, Alberta, T6G 2N8</s2><s3>CAN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Edmonton, Alberta, T6G 2N8</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">08-0429312</idno><date when="2008">2008</date><idno type="stanalyst">PASCAL 08-0429312 INIST</idno><idno type="RBID">Pascal:08-0429312</idno><idno type="wicri:Area/PascalFrancis/Corpus">000254</idno><idno type="wicri:Area/PascalFrancis/Curation">000734</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000300</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000300</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">A rapid point of care immunoswab assay for SARS-CoV detection</title><author><name sortKey="Kammila, Sriram" sort="Kammila, Sriram" uniqKey="Kammila S" first="Sriram" last="Kammila">Sriram Kammila</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, 11304-89 Avenue</s1><s2>Edmonton, Alberta, T6G 2N8</s2><s3>CAN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Edmonton, Alberta, T6G 2N8</wicri:noRegion></affiliation></author><author><name sortKey="Das, Dipankar" sort="Das, Dipankar" uniqKey="Das D" first="Dipankar" last="Das">Dipankar Das</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, 11304-89 Avenue</s1><s2>Edmonton, Alberta, T6G 2N8</s2><s3>CAN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Edmonton, Alberta, T6G 2N8</wicri:noRegion></affiliation></author><author><name sortKey="Bhatnagar, Pravin K" sort="Bhatnagar, Pravin K" uniqKey="Bhatnagar P" first="Pravin K." last="Bhatnagar">Pravin K. Bhatnagar</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, 11304-89 Avenue</s1><s2>Edmonton, Alberta, T6G 2N8</s2><s3>CAN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Edmonton, Alberta, T6G 2N8</wicri:noRegion></affiliation></author><author><name sortKey="Sunwoo, Hoon H" sort="Sunwoo, Hoon H" uniqKey="Sunwoo H" first="Hoon H." last="Sunwoo">Hoon H. Sunwoo</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, 11304-89 Avenue</s1><s2>Edmonton, Alberta, T6G 2N8</s2><s3>CAN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Edmonton, Alberta, T6G 2N8</wicri:noRegion></affiliation></author><author><name sortKey="Zayas Zamora, Gustavo" sort="Zayas Zamora, Gustavo" uniqKey="Zayas Zamora G" first="Gustavo" last="Zayas-Zamora">Gustavo Zayas-Zamora</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Mucophysiology Laboratory, 173 Heritage Medical Research Centre, Department of Medicine, Faculty of Medicine & Dentistry, University of Alberta</s1><s2>Alberta, T6G 2S2</s2><s3>CAN</s3><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Alberta, T6G 2S2</wicri:noRegion></affiliation></author><author><name sortKey="King, Malcolm" sort="King, Malcolm" uniqKey="King M" first="Malcolm" last="King">Malcolm King</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Mucophysiology Laboratory, 173 Heritage Medical Research Centre, Department of Medicine, Faculty of Medicine & Dentistry, University of Alberta</s1><s2>Alberta, T6G 2S2</s2><s3>CAN</s3><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Alberta, T6G 2S2</wicri:noRegion></affiliation></author><author><name sortKey="Suresh, Mavanur R" sort="Suresh, Mavanur R" uniqKey="Suresh M" first="Mavanur R." last="Suresh">Mavanur R. Suresh</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, 11304-89 Avenue</s1><s2>Edmonton, Alberta, T6G 2N8</s2><s3>CAN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Edmonton, Alberta, T6G 2N8</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Journal of virological methods</title><title level="j" type="abbreviated">J. virol. methods</title><idno type="ISSN">0166-0934</idno><imprint><date when="2008">2008</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of virological methods</title><title level="j" type="abbreviated">J. virol. methods</title><idno type="ISSN">0166-0934</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bispecific antibody</term><term>Detection</term><term>Method</term><term>Microbiology</term><term>Monoclonal antibody</term><term>Nucleocapsid</term><term>Protein</term><term>Severe acute respiratory syndrome virus</term><term>Virology</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Virus syndrome respiratoire aigu sévère</term><term>Détection</term><term>Anticorps bispécifique</term><term>Anticorps monoclonal</term><term>Nucléocapside</term><term>Protéine</term><term>Microbiologie</term><term>Méthode</term><term>Virologie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The emergence of severe acute respiratory syndrome (SARS) resulted in several outbreaks worldwide. Early tests for diagnosis were not always conclusive in identifying a SARS suspected patient Nucleocapsid protein (NP) is the most predominant virus derived structural protein which is shed in high amounts in serum and nasopharyngeal aspirate during the first week of infection. As part of such efforts, a simple, easy to use immunoswab method was developed by generating a panel of monoclonal antibodies (MAbs), Bispecific MAbs and chicken polyclonal IgY antibody against the SARS-CoV nucleocapsid protein (NP). Employing the MAb-based immunoswab, an NP concentration of 200 pg/mL in saline and pig nasopharyngeal aspirate, and 500 pg/mL in rabbit serum were detected. BsMAb-based immunoswabs detected an NP concentration of 20 pg/mL in saline, 500 pg/mL in rabbit serum and 20-200 pg/mL in pig nasopharyngeal aspirate. Polyclonal IgY-based immunoswabs detected an NP concentration of 10 pg/mL in pig nasopharyngeal aspirate providing the most sensitive SARS point of care assay. Results show that the robust immunoswab method of detecting SARS-CoV NP antigen can be developed into an easy and effective way of identifying SARS suspected individuals during a future SARS epidemic, thereby reducing and containing the transmission. The key feature of this simple immunoswab diagnostic assay is its ability to detect the presence of the SARS-CoV antigen within 45-60 min with the availability of the body fluid samples.</div></front></TEI><inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0166-0934</s0></fA01><fA02 i1="01"><s0>JVMEDH</s0></fA02><fA03 i2="1"><s0>J. virol. methods</s0></fA03><fA05><s2>152</s2></fA05><fA06><s2>1-2</s2></fA06><fA08 i1="01" i2="1" l="ENG"><s1>A rapid point of care immunoswab assay for SARS-CoV detection</s1></fA08><fA11 i1="01" i2="1"><s1>KAMMILA (Sriram)</s1></fA11><fA11 i1="02" i2="1"><s1>DAS (Dipankar)</s1></fA11><fA11 i1="03" i2="1"><s1>BHATNAGAR (Pravin K.)</s1></fA11><fA11 i1="04" i2="1"><s1>SUNWOO (Hoon H.)</s1></fA11><fA11 i1="05" i2="1"><s1>ZAYAS-ZAMORA (Gustavo)</s1></fA11><fA11 i1="06" i2="1"><s1>KING (Malcolm)</s1></fA11><fA11 i1="07" i2="1"><s1>SURESH (Mavanur R.)</s1></fA11><fA14 i1="01"><s1>Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, 11304-89 Avenue</s1><s2>Edmonton, Alberta, T6G 2N8</s2><s3>CAN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>7 aut.</sZ></fA14><fA14 i1="02"><s1>Mucophysiology Laboratory, 173 Heritage Medical Research Centre, Department of Medicine, Faculty of Medicine & Dentistry, University of Alberta</s1><s2>Alberta, T6G 2S2</s2><s3>CAN</s3><sZ>5 aut.</sZ><sZ>6 aut.</sZ></fA14><fA20><s1>77-84</s1></fA20><fA21><s1>2008</s1></fA21><fA23 i1="01"><s0>ENG</s0></fA23><fA43 i1="01"><s1>INIST</s1><s2>18295</s2><s5>354000197460570130</s5></fA43><fA44><s0>0000</s0><s1>© 2008 INIST-CNRS. All rights reserved.</s1></fA44><fA45><s0>1 p.1/2</s0></fA45><fA47 i1="01" i2="1"><s0>08-0429312</s0></fA47><fA60><s1>P</s1></fA60><fA61><s0>A</s0></fA61><fA64 i1="01" i2="1"><s0>Journal of virological methods</s0></fA64><fA66 i1="01"><s0>NLD</s0></fA66><fC01 i1="01" l="ENG"><s0>The emergence of severe acute respiratory syndrome (SARS) resulted in several outbreaks worldwide. Early tests for diagnosis were not always conclusive in identifying a SARS suspected patient Nucleocapsid protein (NP) is the most predominant virus derived structural protein which is shed in high amounts in serum and nasopharyngeal aspirate during the first week of infection. As part of such efforts, a simple, easy to use immunoswab method was developed by generating a panel of monoclonal antibodies (MAbs), Bispecific MAbs and chicken polyclonal IgY antibody against the SARS-CoV nucleocapsid protein (NP). Employing the MAb-based immunoswab, an NP concentration of 200 pg/mL in saline and pig nasopharyngeal aspirate, and 500 pg/mL in rabbit serum were detected. BsMAb-based immunoswabs detected an NP concentration of 20 pg/mL in saline, 500 pg/mL in rabbit serum and 20-200 pg/mL in pig nasopharyngeal aspirate. Polyclonal IgY-based immunoswabs detected an NP concentration of 10 pg/mL in pig nasopharyngeal aspirate providing the most sensitive SARS point of care assay. Results show that the robust immunoswab method of detecting SARS-CoV NP antigen can be developed into an easy and effective way of identifying SARS suspected individuals during a future SARS epidemic, thereby reducing and containing the transmission. The key feature of this simple immunoswab diagnostic assay is its ability to detect the presence of the SARS-CoV antigen within 45-60 min with the availability of the body fluid samples.</s0></fC01><fC02 i1="01" i2="X"><s0>002A05C09</s0></fC02><fC03 i1="01" i2="X" l="FRE"><s0>Virus syndrome respiratoire aigu sévère</s0><s2>NW</s2><s5>01</s5></fC03><fC03 i1="01" i2="X" l="ENG"><s0>Severe acute respiratory syndrome virus</s0><s2>NW</s2><s5>01</s5></fC03><fC03 i1="01" i2="X" l="SPA"><s0>Severe acute respiratory syndrome virus</s0><s2>NW</s2><s5>01</s5></fC03><fC03 i1="02" i2="X" l="FRE"><s0>Détection</s0><s5>05</s5></fC03><fC03 i1="02" i2="X" l="ENG"><s0>Detection</s0><s5>05</s5></fC03><fC03 i1="02" i2="X" l="SPA"><s0>Detección</s0><s5>05</s5></fC03><fC03 i1="03" i2="X" l="FRE"><s0>Anticorps bispécifique</s0><s5>06</s5></fC03><fC03 i1="03" i2="X" l="ENG"><s0>Bispecific antibody</s0><s5>06</s5></fC03><fC03 i1="03" i2="X" l="SPA"><s0>Anticuerpo biespecífico</s0><s5>06</s5></fC03><fC03 i1="04" i2="X" l="FRE"><s0>Anticorps monoclonal</s0><s5>07</s5></fC03><fC03 i1="04" i2="X" l="ENG"><s0>Monoclonal antibody</s0><s5>07</s5></fC03><fC03 i1="04" i2="X" l="SPA"><s0>Anticuerpo monoclonal</s0><s5>07</s5></fC03><fC03 i1="05" i2="X" l="FRE"><s0>Nucléocapside</s0><s5>08</s5></fC03><fC03 i1="05" i2="X" l="ENG"><s0>Nucleocapsid</s0><s5>08</s5></fC03><fC03 i1="05" i2="X" l="SPA"><s0>Nucleocápside</s0><s5>08</s5></fC03><fC03 i1="06" i2="X" l="FRE"><s0>Protéine</s0><s5>09</s5></fC03><fC03 i1="06" i2="X" l="ENG"><s0>Protein</s0><s5>09</s5></fC03><fC03 i1="06" i2="X" l="SPA"><s0>Proteína</s0><s5>09</s5></fC03><fC03 i1="07" i2="X" l="FRE"><s0>Microbiologie</s0><s5>10</s5></fC03><fC03 i1="07" i2="X" l="ENG"><s0>Microbiology</s0><s5>10</s5></fC03><fC03 i1="07" i2="X" l="SPA"><s0>Microbiología</s0><s5>10</s5></fC03><fC03 i1="08" i2="X" l="FRE"><s0>Méthode</s0><s5>11</s5></fC03><fC03 i1="08" i2="X" l="ENG"><s0>Method</s0><s5>11</s5></fC03><fC03 i1="08" i2="X" l="SPA"><s0>Método</s0><s5>11</s5></fC03><fC03 i1="09" i2="X" l="FRE"><s0>Virologie</s0><s5>12</s5></fC03><fC03 i1="09" i2="X" l="ENG"><s0>Virology</s0><s5>12</s5></fC03><fC03 i1="09" i2="X" l="SPA"><s0>Virología</s0><s5>12</s5></fC03><fC07 i1="01" i2="X" l="FRE"><s0>Coronavirus</s0><s2>NW</s2></fC07><fC07 i1="01" i2="X" l="ENG"><s0>Coronavirus</s0><s2>NW</s2></fC07><fC07 i1="01" i2="X" l="SPA"><s0>Coronavirus</s0><s2>NW</s2></fC07><fC07 i1="02" i2="X" l="FRE"><s0>Coronaviridae</s0><s2>NW</s2></fC07><fC07 i1="02" i2="X" l="ENG"><s0>Coronaviridae</s0><s2>NW</s2></fC07><fC07 i1="02" i2="X" l="SPA"><s0>Coronaviridae</s0><s2>NW</s2></fC07><fC07 i1="03" i2="X" l="FRE"><s0>Nidovirales</s0><s2>NW</s2></fC07><fC07 i1="03" i2="X" l="ENG"><s0>Nidovirales</s0><s2>NW</s2></fC07><fC07 i1="03" i2="X" l="SPA"><s0>Nidovirales</s0><s2>NW</s2></fC07><fC07 i1="04" i2="X" l="FRE"><s0>Virus</s0><s2>NW</s2></fC07><fC07 i1="04" i2="X" l="ENG"><s0>Virus</s0><s2>NW</s2></fC07><fC07 i1="04" i2="X" l="SPA"><s0>Virus</s0><s2>NW</s2></fC07><fN21><s1>280</s1></fN21><fN44 i1="01"><s1>OTO</s1></fN44><fN82><s1>OTO</s1></fN82></pA></standard></inist><affiliations><list><country><li>Canada</li></country></list><tree><country name="Canada"><noRegion><name sortKey="Kammila, Sriram" sort="Kammila, Sriram" uniqKey="Kammila S" first="Sriram" last="Kammila">Sriram Kammila</name></noRegion><name sortKey="Bhatnagar, Pravin K" sort="Bhatnagar, Pravin K" uniqKey="Bhatnagar P" first="Pravin K." last="Bhatnagar">Pravin K. Bhatnagar</name><name sortKey="Das, Dipankar" sort="Das, Dipankar" uniqKey="Das D" first="Dipankar" last="Das">Dipankar Das</name><name sortKey="King, Malcolm" sort="King, Malcolm" uniqKey="King M" first="Malcolm" last="King">Malcolm King</name><name sortKey="Sunwoo, Hoon H" sort="Sunwoo, Hoon H" uniqKey="Sunwoo H" first="Hoon H." last="Sunwoo">Hoon H. Sunwoo</name><name sortKey="Suresh, Mavanur R" sort="Suresh, Mavanur R" uniqKey="Suresh M" first="Mavanur R." last="Suresh">Mavanur R. Suresh</name><name sortKey="Zayas Zamora, Gustavo" sort="Zayas Zamora, Gustavo" uniqKey="Zayas Zamora G" first="Gustavo" last="Zayas-Zamora">Gustavo Zayas-Zamora</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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