Detection of Antibodies Against SARS-Coronavirus Using Recombinant Truncated Nucleocapsid Proteins by ELISA
Identifieur interne : 000289 ( PascalFrancis/Checkpoint ); précédent : 000288; suivant : 000290Detection of Antibodies Against SARS-Coronavirus Using Recombinant Truncated Nucleocapsid Proteins by ELISA
Auteurs : Hyun-Kyoung Lee [Corée du Sud] ; Byoung-Hee Lee [Corée du Sud] ; NOTONKUMAR DUTTA [Corée du Sud] ; Seung-Hyeok Seok [Corée du Sud] ; Min-Won Back [Corée du Sud] ; Hui-Young Lee [Corée du Sud] ; Dong-Jae Kim [Corée du Sud] ; Yi-Rang Na [Corée du Sud] ; Kyoung-Jin Noh [Corée du Sud] ; Sung-Hoon Park [Corée du Sud] ; Hiroaki Kariwa [Japon] ; Mina Nakauchi [Japon] ; LE QUYNH MAI [Viêt Nam] ; Suk-Jin Heo [Corée du Sud] ; Jae-Hak Park [Corée du Sud]Source :
- Journal of microbiology and biotechnology [ 1017-7825 ] ; 2008.
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Abstract
Severe acute respiratory syndrome (SARS) is a life-threatening emerging respiratory disease caused by the coronavirus, SARS-CoV. The nucleocapsid (N) protein of SARS-CoV is highly antigenic and may be a suitable candidate for diagnostic applications. We constructed truncated recombinant N proteins (N1 [1-422 aa], N2 [1-109 aa], and N3 [110-422 aa]) and determined their antigenicity by Western blotting using convalescent SARS serum. The recombinants containing N1 and N3 reacted with convalescent SARS serum in Western blotting. However, the recombinant with N2 did not. In ELISA using N1 or N3 as the antigens, positive results were observed in 10 of 10 (100%) SARS-CoV-positive human sera. None of 50 healthy sera gave positive results in either assay. These data indicate that the ELISA using N1 or N3 has high sensitivity and specificity. These results suggest that the middle or C-terminal region of the SARS N protein is important for eliciting antibodies against SARS-CoV during the immune response, and ELISA reactions using N1 or N3 may be a valuable tool for SARS diagnosis.
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sortKey="Lee, Byoung Hee" sort="Lee, Byoung Hee" uniqKey="Lee B" first="Byoung-Hee" last="Lee">Byoung-Hee Lee</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Division of Vertebrates Research, National Institute of Biological Resources</s1><s2>Incheon 404-170</s2><s3>KOR</s3><sZ>2 aut.</sZ></inist:fA14><country>Corée du Sud</country><wicri:noRegion>Incheon 404-170</wicri:noRegion></affiliation></author><author><name sortKey="Notonkumar Dutta" sort="Notonkumar Dutta" uniqKey="Notonkumar Dutta" last="Notonkumar Dutta">NOTONKUMAR DUTTA</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University</s1><s2>Seoul 151-742</s2><s3>KOR</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>15 aut.</sZ></inist:fA14><country>Corée du Sud</country><placeName><settlement 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type="city">Séoul</settlement></placeName></affiliation></author><author><name sortKey="Back, Min Won" sort="Back, Min Won" uniqKey="Back M" first="Min-Won" last="Back">Min-Won Back</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University</s1><s2>Seoul 151-742</s2><s3>KOR</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>15 aut.</sZ></inist:fA14><country>Corée du Sud</country><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName><orgName type="university">Université nationale de Séoul</orgName><placeName><settlement type="city">Séoul</settlement></placeName></affiliation></author><author><name sortKey="Lee, Hui Young" sort="Lee, Hui Young" uniqKey="Lee H" first="Hui-Young" last="Lee">Hui-Young Lee</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University</s1><s2>Seoul 151-742</s2><s3>KOR</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>15 aut.</sZ></inist:fA14><country>Corée du Sud</country><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName><orgName type="university">Université nationale de Séoul</orgName><placeName><settlement type="city">Séoul</settlement></placeName></affiliation></author><author><name sortKey="Kim, Dong Jae" sort="Kim, Dong Jae" uniqKey="Kim D" first="Dong-Jae" last="Kim">Dong-Jae Kim</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University</s1><s2>Seoul 151-742</s2><s3>KOR</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>15 aut.</sZ></inist:fA14><country>Corée du Sud</country><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName><orgName type="university">Université nationale de Séoul</orgName><placeName><settlement type="city">Séoul</settlement></placeName></affiliation></author><author><name sortKey="Na, Yi Rang" sort="Na, Yi Rang" uniqKey="Na Y" first="Yi-Rang" last="Na">Yi-Rang Na</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University</s1><s2>Seoul 151-742</s2><s3>KOR</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>15 aut.</sZ></inist:fA14><country>Corée du Sud</country><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName><orgName type="university">Université nationale de Séoul</orgName><placeName><settlement type="city">Séoul</settlement></placeName></affiliation></author><author><name sortKey="Noh, Kyoung Jin" sort="Noh, Kyoung Jin" uniqKey="Noh K" first="Kyoung-Jin" last="Noh">Kyoung-Jin Noh</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University</s1><s2>Seoul 151-742</s2><s3>KOR</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>15 aut.</sZ></inist:fA14><country>Corée du Sud</country><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName><orgName type="university">Université nationale de Séoul</orgName><placeName><settlement type="city">Séoul</settlement></placeName></affiliation></author><author><name sortKey="Park, Sung Hoon" sort="Park, Sung Hoon" uniqKey="Park S" first="Sung-Hoon" last="Park">Sung-Hoon Park</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University</s1><s2>Seoul 151-742</s2><s3>KOR</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>15 aut.</sZ></inist:fA14><country>Corée du Sud</country><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName><orgName type="university">Université nationale de Séoul</orgName><placeName><settlement type="city">Séoul</settlement></placeName></affiliation></author><author><name sortKey="Kariwa, Hiroaki" sort="Kariwa, Hiroaki" uniqKey="Kariwa H" first="Hiroaki" last="Kariwa">Hiroaki Kariwa</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Laboratory of Public Health, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University</s1><s2>Sapporo 060-0818</s2><s3>JPN</s3><sZ>11 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Sapporo 060-0818</wicri:noRegion></affiliation></author><author><name sortKey="Nakauchi, Mina" sort="Nakauchi, Mina" uniqKey="Nakauchi M" first="Mina" last="Nakauchi">Mina Nakauchi</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Laboratory of Public Health, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University</s1><s2>Sapporo 060-0818</s2><s3>JPN</s3><sZ>11 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Sapporo 060-0818</wicri:noRegion></affiliation></author><author><name sortKey="Le Quynh Mai" sort="Le Quynh Mai" uniqKey="Le Quynh Mai" last="Le Quynh Mai">LE QUYNH MAI</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Virology Department, National Institute of Hygiene and Epidemiology</s1><s2>Yersin Street, Hanoi 10000</s2><s3>VNM</s3><sZ>13 aut.</sZ></inist:fA14><country>Viêt Nam</country><wicri:noRegion>Yersin Street, Hanoi 10000</wicri:noRegion></affiliation></author><author><name sortKey="Heo, Suk Jin" sort="Heo, Suk Jin" uniqKey="Heo S" first="Suk-Jin" last="Heo">Suk-Jin Heo</name><affiliation wicri:level="1"><inist:fA14 i1="05"><s1>Biological Diagnostic Products Team, Biologics Headquarters, Korea Food and Drug Administration</s1><s2>Seoul 122-704</s2><s3>KOR</s3><sZ>14 aut.</sZ></inist:fA14><country>Corée du Sud</country><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Park, Jae Hak" sort="Park, Jae Hak" uniqKey="Park J" first="Jae-Hak" last="Park">Jae-Hak Park</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University</s1><s2>Seoul 151-742</s2><s3>KOR</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>15 aut.</sZ></inist:fA14><country>Corée du Sud</country><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName><orgName type="university">Université nationale de Séoul</orgName><placeName><settlement type="city">Séoul</settlement></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">09-0101127</idno><date when="2008">2008</date><idno type="stanalyst">PASCAL 09-0101127 INIST</idno><idno type="RBID">Pascal:09-0101127</idno><idno type="wicri:Area/PascalFrancis/Corpus">000235</idno><idno type="wicri:Area/PascalFrancis/Curation">000753</idno><idno 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type="university">Université nationale de Séoul</orgName><placeName><settlement type="city">Séoul</settlement></placeName></affiliation></author><author><name sortKey="Lee, Byoung Hee" sort="Lee, Byoung Hee" uniqKey="Lee B" first="Byoung-Hee" last="Lee">Byoung-Hee Lee</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Division of Vertebrates Research, National Institute of Biological Resources</s1><s2>Incheon 404-170</s2><s3>KOR</s3><sZ>2 aut.</sZ></inist:fA14><country>Corée du Sud</country><wicri:noRegion>Incheon 404-170</wicri:noRegion></affiliation></author><author><name sortKey="Notonkumar Dutta" sort="Notonkumar Dutta" uniqKey="Notonkumar Dutta" last="Notonkumar Dutta">NOTONKUMAR DUTTA</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University</s1><s2>Seoul 151-742</s2><s3>KOR</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>15 aut.</sZ></inist:fA14><country>Corée du Sud</country><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName><orgName type="university">Université nationale de Séoul</orgName><placeName><settlement type="city">Séoul</settlement></placeName></affiliation></author><author><name sortKey="Seok, Seung Hyeok" sort="Seok, Seung Hyeok" uniqKey="Seok S" first="Seung-Hyeok" last="Seok">Seung-Hyeok Seok</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University</s1><s2>Seoul 151-742</s2><s3>KOR</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>15 aut.</sZ></inist:fA14><country>Corée du Sud</country><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName><orgName type="university">Université nationale de Séoul</orgName><placeName><settlement type="city">Séoul</settlement></placeName></affiliation></author><author><name sortKey="Back, Min Won" sort="Back, Min Won" uniqKey="Back M" first="Min-Won" last="Back">Min-Won Back</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University</s1><s2>Seoul 151-742</s2><s3>KOR</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>15 aut.</sZ></inist:fA14><country>Corée du Sud</country><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName><orgName type="university">Université nationale de Séoul</orgName><placeName><settlement type="city">Séoul</settlement></placeName></affiliation></author><author><name sortKey="Lee, Hui Young" sort="Lee, Hui Young" uniqKey="Lee H" first="Hui-Young" last="Lee">Hui-Young Lee</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University</s1><s2>Seoul 151-742</s2><s3>KOR</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>15 aut.</sZ></inist:fA14><country>Corée du Sud</country><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName><orgName type="university">Université nationale de Séoul</orgName><placeName><settlement type="city">Séoul</settlement></placeName></affiliation></author><author><name sortKey="Kim, Dong Jae" sort="Kim, Dong Jae" uniqKey="Kim D" first="Dong-Jae" last="Kim">Dong-Jae Kim</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University</s1><s2>Seoul 151-742</s2><s3>KOR</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>15 aut.</sZ></inist:fA14><country>Corée du Sud</country><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName><orgName type="university">Université nationale de Séoul</orgName><placeName><settlement type="city">Séoul</settlement></placeName></affiliation></author><author><name sortKey="Na, Yi Rang" sort="Na, Yi Rang" uniqKey="Na Y" first="Yi-Rang" last="Na">Yi-Rang Na</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University</s1><s2>Seoul 151-742</s2><s3>KOR</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>15 aut.</sZ></inist:fA14><country>Corée du Sud</country><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName><orgName type="university">Université nationale de Séoul</orgName><placeName><settlement type="city">Séoul</settlement></placeName></affiliation></author><author><name sortKey="Noh, Kyoung Jin" sort="Noh, Kyoung Jin" uniqKey="Noh K" first="Kyoung-Jin" last="Noh">Kyoung-Jin Noh</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University</s1><s2>Seoul 151-742</s2><s3>KOR</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>15 aut.</sZ></inist:fA14><country>Corée du Sud</country><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName><orgName type="university">Université nationale de Séoul</orgName><placeName><settlement type="city">Séoul</settlement></placeName></affiliation></author><author><name sortKey="Park, Sung Hoon" sort="Park, Sung Hoon" uniqKey="Park S" first="Sung-Hoon" last="Park">Sung-Hoon Park</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University</s1><s2>Seoul 151-742</s2><s3>KOR</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>15 aut.</sZ></inist:fA14><country>Corée du Sud</country><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName><orgName type="university">Université nationale de Séoul</orgName><placeName><settlement type="city">Séoul</settlement></placeName></affiliation></author><author><name sortKey="Kariwa, Hiroaki" sort="Kariwa, Hiroaki" uniqKey="Kariwa H" first="Hiroaki" last="Kariwa">Hiroaki Kariwa</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Laboratory of Public Health, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University</s1><s2>Sapporo 060-0818</s2><s3>JPN</s3><sZ>11 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Sapporo 060-0818</wicri:noRegion></affiliation></author><author><name sortKey="Nakauchi, Mina" sort="Nakauchi, Mina" uniqKey="Nakauchi M" first="Mina" last="Nakauchi">Mina Nakauchi</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Laboratory of Public Health, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University</s1><s2>Sapporo 060-0818</s2><s3>JPN</s3><sZ>11 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Sapporo 060-0818</wicri:noRegion></affiliation></author><author><name sortKey="Le Quynh Mai" sort="Le Quynh Mai" uniqKey="Le Quynh Mai" last="Le Quynh Mai">LE QUYNH MAI</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Virology Department, National Institute of Hygiene and Epidemiology</s1><s2>Yersin Street, Hanoi 10000</s2><s3>VNM</s3><sZ>13 aut.</sZ></inist:fA14><country>Viêt Nam</country><wicri:noRegion>Yersin Street, Hanoi 10000</wicri:noRegion></affiliation></author><author><name sortKey="Heo, Suk Jin" sort="Heo, Suk Jin" uniqKey="Heo S" first="Suk-Jin" last="Heo">Suk-Jin Heo</name><affiliation wicri:level="1"><inist:fA14 i1="05"><s1>Biological Diagnostic Products Team, Biologics Headquarters, Korea Food and Drug Administration</s1><s2>Seoul 122-704</s2><s3>KOR</s3><sZ>14 aut.</sZ></inist:fA14><country>Corée du Sud</country><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Park, Jae Hak" sort="Park, Jae Hak" uniqKey="Park J" first="Jae-Hak" last="Park">Jae-Hak Park</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University</s1><s2>Seoul 151-742</s2><s3>KOR</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>15 aut.</sZ></inist:fA14><country>Corée du Sud</country><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName><orgName type="university">Université nationale de Séoul</orgName><placeName><settlement type="city">Séoul</settlement></placeName></affiliation></author></analytic><series><title level="j" type="main">Journal of microbiology and biotechnology</title><title level="j" type="abbreviated">J. microbiol. biotechnol.</title><idno type="ISSN">1017-7825</idno><imprint><date when="2008">2008</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of microbiology and biotechnology</title><title level="j" type="abbreviated">J. microbiol. biotechnol.</title><idno type="ISSN">1017-7825</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibody</term><term>Coronavirus</term><term>Detection</term><term>ELISA assay</term><term>Nucleocapsid</term><term>Recombinant protein</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Détection</term><term>Anticorps</term><term>Protéine recombinante</term><term>Nucléocapside</term><term>Technique ELISA</term><term>Coronavirus</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) is a life-threatening emerging respiratory disease caused by the coronavirus, SARS-CoV. The nucleocapsid (N) protein of SARS-CoV is highly antigenic and may be a suitable candidate for diagnostic applications. We constructed truncated recombinant N proteins (N1 [1-422 aa], N2 [1-109 aa], and N3 [110-422 aa]) and determined their antigenicity by Western blotting using convalescent SARS serum. The recombinants containing N1 and N3 reacted with convalescent SARS serum in Western blotting. However, the recombinant with N2 did not. In ELISA using N1 or N3 as the antigens, positive results were observed in 10 of 10 (100%) SARS-CoV-positive human sera. None of 50 healthy sera gave positive results in either assay. These data indicate that the ELISA using N1 or N3 has high sensitivity and specificity. These results suggest that the middle or C-terminal region of the SARS N protein is important for eliciting antibodies against SARS-CoV during the immune response, and ELISA reactions using N1 or N3 may be a valuable tool for SARS diagnosis.</div></front></TEI><inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>1017-7825</s0></fA01><fA03 i2="1"><s0>J. microbiol. biotechnol.</s0></fA03><fA05><s2>18</s2></fA05><fA06><s2>10</s2></fA06><fA08 i1="01" i2="1" l="ENG"><s1>Detection of Antibodies Against SARS-Coronavirus Using Recombinant Truncated Nucleocapsid Proteins by ELISA</s1></fA08><fA11 i1="01" i2="1"><s1>LEE (Hyun-Kyoung)</s1></fA11><fA11 i1="02" i2="1"><s1>LEE (Byoung-Hee)</s1></fA11><fA11 i1="03" i2="1"><s1>NOTONKUMAR DUTTA</s1></fA11><fA11 i1="04" i2="1"><s1>SEOK (Seung-Hyeok)</s1></fA11><fA11 i1="05" i2="1"><s1>BACK (Min-Won)</s1></fA11><fA11 i1="06" i2="1"><s1>LEE (Hui-Young)</s1></fA11><fA11 i1="07" i2="1"><s1>KIM (Dong-Jae)</s1></fA11><fA11 i1="08" i2="1"><s1>NA (Yi-Rang)</s1></fA11><fA11 i1="09" i2="1"><s1>NOH (Kyoung-Jin)</s1></fA11><fA11 i1="10" i2="1"><s1>PARK (Sung-Hoon)</s1></fA11><fA11 i1="11" i2="1"><s1>KARIWA (Hiroaki)</s1></fA11><fA11 i1="12" i2="1"><s1>NAKAUCHI (Mina)</s1></fA11><fA11 i1="13" i2="1"><s1>LE QUYNH MAI</s1></fA11><fA11 i1="14" i2="1"><s1>HEO (Suk-Jin)</s1></fA11><fA11 i1="15" i2="1"><s1>PARK (Jae-Hak)</s1></fA11><fA14 i1="01"><s1>Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University</s1><s2>Seoul 151-742</s2><s3>KOR</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>15 aut.</sZ></fA14><fA14 i1="02"><s1>Division of Vertebrates Research, National Institute of Biological Resources</s1><s2>Incheon 404-170</s2><s3>KOR</s3><sZ>2 aut.</sZ></fA14><fA14 i1="03"><s1>Laboratory of Public Health, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University</s1><s2>Sapporo 060-0818</s2><s3>JPN</s3><sZ>11 aut.</sZ><sZ>12 aut.</sZ></fA14><fA14 i1="04"><s1>Virology Department, National Institute of Hygiene and Epidemiology</s1><s2>Yersin Street, Hanoi 10000</s2><s3>VNM</s3><sZ>13 aut.</sZ></fA14><fA14 i1="05"><s1>Biological Diagnostic Products Team, Biologics Headquarters, Korea Food and Drug Administration</s1><s2>Seoul 122-704</s2><s3>KOR</s3><sZ>14 aut.</sZ></fA14><fA20><s1>1717-1721</s1></fA20><fA21><s1>2008</s1></fA21><fA23 i1="01"><s0>ENG</s0></fA23><fA43 i1="01"><s1>INIST</s1><s2>26482</s2><s5>354000186454660170</s5></fA43><fA44><s0>0000</s0><s1>© 2009 INIST-CNRS. All rights reserved.</s1></fA44><fA45><s0>18 ref.</s0></fA45><fA47 i1="01" i2="1"><s0>09-0101127</s0></fA47><fA60><s1>P</s1></fA60><fA61><s0>A</s0></fA61><fA64 i1="01" i2="1"><s0>Journal of microbiology and biotechnology</s0></fA64><fA66 i1="01"><s0>KOR</s0></fA66><fC01 i1="01" l="ENG"><s0>Severe acute respiratory syndrome (SARS) is a life-threatening emerging respiratory disease caused by the coronavirus, SARS-CoV. The nucleocapsid (N) protein of SARS-CoV is highly antigenic and may be a suitable candidate for diagnostic applications. We constructed truncated recombinant N proteins (N1 [1-422 aa], N2 [1-109 aa], and N3 [110-422 aa]) and determined their antigenicity by Western blotting using convalescent SARS serum. The recombinants containing N1 and N3 reacted with convalescent SARS serum in Western blotting. However, the recombinant with N2 did not. In ELISA using N1 or N3 as the antigens, positive results were observed in 10 of 10 (100%) SARS-CoV-positive human sera. None of 50 healthy sera gave positive results in either assay. These data indicate that the ELISA using N1 or N3 has high sensitivity and specificity. These results suggest that the middle or C-terminal region of the SARS N protein is important for eliciting antibodies against SARS-CoV during the immune response, and ELISA reactions using N1 or N3 may be a valuable tool for SARS diagnosis.</s0></fC01><fC02 i1="01" i2="X"><s0>002A31</s0></fC02><fC02 i1="02" i2="X"><s0>215</s0></fC02><fC03 i1="01" i2="X" l="FRE"><s0>Détection</s0><s5>01</s5></fC03><fC03 i1="01" i2="X" l="ENG"><s0>Detection</s0><s5>01</s5></fC03><fC03 i1="01" i2="X" l="SPA"><s0>Detección</s0><s5>01</s5></fC03><fC03 i1="02" i2="X" l="FRE"><s0>Anticorps</s0><s5>02</s5></fC03><fC03 i1="02" i2="X" l="ENG"><s0>Antibody</s0><s5>02</s5></fC03><fC03 i1="02" i2="X" l="SPA"><s0>Anticuerpo</s0><s5>02</s5></fC03><fC03 i1="03" i2="X" l="FRE"><s0>Protéine recombinante</s0><s5>10</s5></fC03><fC03 i1="03" i2="X" l="ENG"><s0>Recombinant protein</s0><s5>10</s5></fC03><fC03 i1="03" i2="X" l="SPA"><s0>Proteína recombinante</s0><s5>10</s5></fC03><fC03 i1="04" i2="X" l="FRE"><s0>Nucléocapside</s0><s5>11</s5></fC03><fC03 i1="04" i2="X" l="ENG"><s0>Nucleocapsid</s0><s5>11</s5></fC03><fC03 i1="04" i2="X" l="SPA"><s0>Nucleocápside</s0><s5>11</s5></fC03><fC03 i1="05" i2="X" l="FRE"><s0>Technique ELISA</s0><s5>12</s5></fC03><fC03 i1="05" i2="X" l="ENG"><s0>ELISA assay</s0><s5>12</s5></fC03><fC03 i1="05" i2="X" l="SPA"><s0>Técnica ELISA</s0><s5>12</s5></fC03><fC03 i1="06" i2="X" l="FRE"><s0>Coronavirus</s0><s2>NW</s2><s5>13</s5></fC03><fC03 i1="06" i2="X" l="ENG"><s0>Coronavirus</s0><s2>NW</s2><s5>13</s5></fC03><fC03 i1="06" i2="X" l="SPA"><s0>Coronavirus</s0><s2>NW</s2><s5>13</s5></fC03><fC07 i1="01" i2="X" l="FRE"><s0>Coronaviridae</s0><s2>NW</s2></fC07><fC07 i1="01" i2="X" l="ENG"><s0>Coronaviridae</s0><s2>NW</s2></fC07><fC07 i1="01" i2="X" l="SPA"><s0>Coronaviridae</s0><s2>NW</s2></fC07><fC07 i1="02" i2="X" l="FRE"><s0>Nidovirales</s0><s2>NW</s2></fC07><fC07 i1="02" i2="X" l="ENG"><s0>Nidovirales</s0><s2>NW</s2></fC07><fC07 i1="02" i2="X" l="SPA"><s0>Nidovirales</s0><s2>NW</s2></fC07><fC07 i1="03" i2="X" l="FRE"><s0>Virus</s0><s2>NW</s2></fC07><fC07 i1="03" i2="X" l="ENG"><s0>Virus</s0><s2>NW</s2></fC07><fC07 i1="03" i2="X" l="SPA"><s0>Virus</s0><s2>NW</s2></fC07><fN21><s1>068</s1></fN21><fN44 i1="01"><s1>OTO</s1></fN44><fN82><s1>OTO</s1></fN82></pA></standard></inist><affiliations><list><country><li>Corée du Sud</li><li>Japon</li><li>Viêt Nam</li></country><region><li>Région capitale de Séoul</li></region><settlement><li>Séoul</li></settlement><orgName><li>Université nationale de Séoul</li></orgName></list><tree><country name="Corée du Sud"><region name="Région capitale de Séoul"><name sortKey="Lee, Hyun Kyoung" sort="Lee, Hyun Kyoung" uniqKey="Lee H" first="Hyun-Kyoung" last="Lee">Hyun-Kyoung Lee</name></region><name sortKey="Back, Min Won" sort="Back, Min Won" uniqKey="Back M" first="Min-Won" last="Back">Min-Won Back</name><name sortKey="Heo, Suk Jin" sort="Heo, Suk Jin" uniqKey="Heo S" first="Suk-Jin" last="Heo">Suk-Jin Heo</name><name sortKey="Kim, Dong Jae" sort="Kim, Dong Jae" uniqKey="Kim D" first="Dong-Jae" last="Kim">Dong-Jae Kim</name><name sortKey="Lee, Byoung Hee" sort="Lee, Byoung Hee" uniqKey="Lee B" first="Byoung-Hee" last="Lee">Byoung-Hee Lee</name><name sortKey="Lee, Hui Young" sort="Lee, Hui Young" uniqKey="Lee H" first="Hui-Young" last="Lee">Hui-Young Lee</name><name sortKey="Na, Yi Rang" sort="Na, Yi Rang" uniqKey="Na Y" first="Yi-Rang" last="Na">Yi-Rang Na</name><name sortKey="Noh, Kyoung Jin" sort="Noh, Kyoung Jin" uniqKey="Noh K" first="Kyoung-Jin" last="Noh">Kyoung-Jin Noh</name><name sortKey="Notonkumar Dutta" sort="Notonkumar Dutta" uniqKey="Notonkumar Dutta" last="Notonkumar Dutta">NOTONKUMAR DUTTA</name><name sortKey="Park, Jae Hak" sort="Park, Jae Hak" uniqKey="Park J" first="Jae-Hak" last="Park">Jae-Hak Park</name><name sortKey="Park, Sung Hoon" sort="Park, Sung Hoon" uniqKey="Park S" first="Sung-Hoon" last="Park">Sung-Hoon Park</name><name sortKey="Seok, Seung Hyeok" sort="Seok, Seung Hyeok" uniqKey="Seok S" first="Seung-Hyeok" last="Seok">Seung-Hyeok Seok</name></country><country name="Japon"><noRegion><name sortKey="Kariwa, Hiroaki" sort="Kariwa, Hiroaki" uniqKey="Kariwa H" first="Hiroaki" last="Kariwa">Hiroaki Kariwa</name></noRegion><name sortKey="Nakauchi, Mina" sort="Nakauchi, Mina" uniqKey="Nakauchi M" first="Mina" last="Nakauchi">Mina Nakauchi</name></country><country name="Viêt Nam"><noRegion><name sortKey="Le Quynh Mai" sort="Le Quynh Mai" uniqKey="Le Quynh Mai" last="Le Quynh Mai">LE QUYNH MAI</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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