Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2.
Identifieur interne : 003A82 ( Ncbi/Merge ); précédent : 003A81; suivant : 003A83Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2.
Auteurs : Jesse J. Waggoner ; Victoria Stittleburg ; Renee Pond ; Youssef Saklawi ; Malaya K. Sahoo ; Ahmed Babiker ; Laila Hussaini ; Colleen S. Kraft ; Benjamin A. Pinsky ; Evan J. Anderson ; Nadine RouphaelSource :
- Emerging infectious diseases [ 1080-6059 ] ; 2020.
Abstract
Most reverse transcription PCR protocols for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) include 2-3 targets for detection. We developed a triplex, real-time reverse transcription PCR for SARS-CoV-2 that maintained clinical performance compared with singleplex assays. This protocol could streamline detection and decrease reagent use during current high SARS-CoV-2 testing demands.
DOI: 10.3201/eid2607.201285
PubMed: 32294051
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pubmed:32294051Le document en format XML
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<front><div type="abstract" xml:lang="en">Most reverse transcription PCR protocols for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) include 2-3 targets for detection. We developed a triplex, real-time reverse transcription PCR for SARS-CoV-2 that maintained clinical performance compared with singleplex assays. This protocol could streamline detection and decrease reagent use during current high SARS-CoV-2 testing demands.</div>
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<tree><noCountry><name sortKey="Anderson, Evan J" sort="Anderson, Evan J" uniqKey="Anderson E" first="Evan J" last="Anderson">Evan J. Anderson</name>
<name sortKey="Babiker, Ahmed" sort="Babiker, Ahmed" uniqKey="Babiker A" first="Ahmed" last="Babiker">Ahmed Babiker</name>
<name sortKey="Hussaini, Laila" sort="Hussaini, Laila" uniqKey="Hussaini L" first="Laila" last="Hussaini">Laila Hussaini</name>
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