Handling and Processing of Blood Specimens from Patients with COVID-19 for Safe Studies on Cell Phenotype and Cytokine Storm.
Identifieur interne : 003868 ( Ncbi/Merge ); précédent : 003867; suivant : 003869Handling and Processing of Blood Specimens from Patients with COVID-19 for Safe Studies on Cell Phenotype and Cytokine Storm.
Auteurs : Andrea Cossarizza [Italie] ; Lara Gibellini [Italie] ; Sara De Biasi [Italie] ; Domenico Lo Tartaro [Italie] ; Marco Mattioli [Italie] ; Annamaria Paolini [Italie] ; Lucia Fidanza [Italie] ; Caterina Bellinazzi [Italie] ; Rebecca Borella [Italie] ; Ivana Castaniere [Italie] ; Marianna Meschiari [Italie] ; Marco Sita [Italie] ; Gianrocco Manco [Italie] ; Enrico Clini [Italie] ; Roberta Gelmini [Italie] ; Massimo Girardis [Italie] ; Giovanni Guaraldi [Italie] ; Cristina Mussini [Italie]Source :
- Cytometry. Part A : the journal of the International Society for Analytical Cytology [ 1552-4930 ] ; 2020.
Abstract
The pandemic caused by severe acute respiratory syndrome coronavirus 2 heavily involves all those working in a laboratory. Samples from known infected patients or donors who are considered healthy can arrive, and a colleague might be asymptomatic but able to transmit the virus. Working in a clinical laboratory is posing several safety challenges. Few years ago, International Society for Advancement of Cytometry published guidelines to safely analyze and sort human samples that were revised in these days. We describe the procedures that we have been following since the first patient appeared in Italy, which have only slightly modified our standard one, being all human samples associated with risks. © 2020 International Society for Advancement of Cytometry.
DOI: 10.1002/cyto.a.24009
PubMed: 32275124
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Handling and Processing of Blood Specimens from Patients with COVID-19 for Safe Studies on Cell Phenotype and Cytokine Storm.</title><author><name sortKey="Cossarizza, Andrea" sort="Cossarizza, Andrea" uniqKey="Cossarizza A" first="Andrea" last="Cossarizza">Andrea Cossarizza</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author><author><name sortKey="Gibellini, Lara" sort="Gibellini, Lara" uniqKey="Gibellini L" first="Lara" last="Gibellini">Lara Gibellini</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author><author><name sortKey="De Biasi, Sara" sort="De Biasi, Sara" uniqKey="De Biasi S" first="Sara" last="De Biasi">Sara De Biasi</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, 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sortKey="Fidanza, Lucia" sort="Fidanza, Lucia" uniqKey="Fidanza L" first="Lucia" last="Fidanza">Lucia Fidanza</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author><author><name sortKey="Bellinazzi, Caterina" sort="Bellinazzi, Caterina" uniqKey="Bellinazzi C" first="Caterina" last="Bellinazzi">Caterina Bellinazzi</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author><author><name sortKey="Borella, Rebecca" sort="Borella, Rebecca" uniqKey="Borella R" first="Rebecca" last="Borella">Rebecca Borella</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author><author><name sortKey="Castaniere, Ivana" sort="Castaniere, Ivana" uniqKey="Castaniere I" first="Ivana" last="Castaniere">Ivana Castaniere</name><affiliation wicri:level="1"><nlm:affiliation>Respiratory Diseases Unit, AOU Policlinico and University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Respiratory Diseases Unit, AOU Policlinico and University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author><author><name sortKey="Meschiari, Marianna" sort="Meschiari, Marianna" uniqKey="Meschiari M" first="Marianna" last="Meschiari">Marianna Meschiari</name><affiliation wicri:level="1"><nlm:affiliation>Infectious Diseases Clinics, AOU Policlinico and University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Infectious Diseases Clinics, AOU Policlinico and University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author><author><name sortKey="Sita, Marco" sort="Sita, Marco" uniqKey="Sita M" first="Marco" last="Sita">Marco Sita</name><affiliation wicri:level="1"><nlm:affiliation>Department of Anesthesia and Intensive Care Unit, AOU Policlinico and University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Anesthesia and Intensive Care Unit, AOU Policlinico and University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author><author><name sortKey="Manco, Gianrocco" sort="Manco, Gianrocco" uniqKey="Manco G" first="Gianrocco" last="Manco">Gianrocco Manco</name><affiliation wicri:level="1"><nlm:affiliation>Department of Surgery, AOU Policlinico and University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Surgery, AOU Policlinico and University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author><author><name sortKey="Clini, Enrico" sort="Clini, Enrico" uniqKey="Clini E" first="Enrico" last="Clini">Enrico Clini</name><affiliation wicri:level="1"><nlm:affiliation>Respiratory Diseases Unit, AOU Policlinico and University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Respiratory Diseases Unit, AOU Policlinico and University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author><author><name sortKey="Gelmini, Roberta" sort="Gelmini, Roberta" uniqKey="Gelmini R" first="Roberta" last="Gelmini">Roberta Gelmini</name><affiliation wicri:level="1"><nlm:affiliation>Department of Surgery, AOU Policlinico and University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Surgery, AOU Policlinico and University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author><author><name sortKey="Girardis, Massimo" sort="Girardis, Massimo" uniqKey="Girardis M" first="Massimo" last="Girardis">Massimo Girardis</name><affiliation wicri:level="1"><nlm:affiliation>Department of Anesthesia and Intensive Care Unit, AOU Policlinico and University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Anesthesia and Intensive Care Unit, AOU Policlinico and University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author><author><name sortKey="Guaraldi, Giovanni" sort="Guaraldi, Giovanni" uniqKey="Guaraldi G" first="Giovanni" last="Guaraldi">Giovanni 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last="Cossarizza">Andrea Cossarizza</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author><author><name sortKey="Gibellini, Lara" sort="Gibellini, Lara" uniqKey="Gibellini L" first="Lara" last="Gibellini">Lara Gibellini</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author><author><name sortKey="De Biasi, Sara" sort="De Biasi, Sara" uniqKey="De Biasi S" first="Sara" last="De Biasi">Sara De Biasi</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author><author><name sortKey="Lo Tartaro, Domenico" sort="Lo Tartaro, Domenico" uniqKey="Lo Tartaro D" first="Domenico" last="Lo Tartaro">Domenico Lo Tartaro</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author><author><name sortKey="Mattioli, Marco" sort="Mattioli, Marco" uniqKey="Mattioli M" first="Marco" last="Mattioli">Marco Mattioli</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author><author><name sortKey="Paolini, Annamaria" sort="Paolini, Annamaria" uniqKey="Paolini A" first="Annamaria" last="Paolini">Annamaria Paolini</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author><author><name sortKey="Fidanza, Lucia" sort="Fidanza, Lucia" uniqKey="Fidanza L" first="Lucia" last="Fidanza">Lucia Fidanza</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author><author><name sortKey="Bellinazzi, Caterina" sort="Bellinazzi, Caterina" uniqKey="Bellinazzi C" first="Caterina" last="Bellinazzi">Caterina Bellinazzi</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author><author><name sortKey="Borella, Rebecca" sort="Borella, Rebecca" uniqKey="Borella R" first="Rebecca" last="Borella">Rebecca Borella</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author><author><name sortKey="Castaniere, Ivana" sort="Castaniere, Ivana" uniqKey="Castaniere I" first="Ivana" last="Castaniere">Ivana Castaniere</name><affiliation wicri:level="1"><nlm:affiliation>Respiratory Diseases Unit, AOU Policlinico and University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Respiratory Diseases Unit, AOU Policlinico and University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author><author><name sortKey="Meschiari, Marianna" sort="Meschiari, Marianna" uniqKey="Meschiari M" first="Marianna" last="Meschiari">Marianna Meschiari</name><affiliation wicri:level="1"><nlm:affiliation>Infectious Diseases Clinics, AOU Policlinico and University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Infectious Diseases Clinics, AOU Policlinico and University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author><author><name sortKey="Sita, Marco" sort="Sita, Marco" uniqKey="Sita M" first="Marco" last="Sita">Marco Sita</name><affiliation wicri:level="1"><nlm:affiliation>Department of Anesthesia and Intensive Care Unit, AOU Policlinico and University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Anesthesia and Intensive Care Unit, AOU Policlinico and University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author><author><name sortKey="Manco, Gianrocco" sort="Manco, Gianrocco" uniqKey="Manco G" first="Gianrocco" last="Manco">Gianrocco Manco</name><affiliation wicri:level="1"><nlm:affiliation>Department of Surgery, AOU Policlinico and University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Surgery, AOU Policlinico and University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author><author><name sortKey="Clini, Enrico" sort="Clini, Enrico" uniqKey="Clini E" first="Enrico" last="Clini">Enrico Clini</name><affiliation wicri:level="1"><nlm:affiliation>Respiratory Diseases Unit, AOU Policlinico and University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Respiratory Diseases Unit, AOU Policlinico and University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author><author><name sortKey="Gelmini, Roberta" sort="Gelmini, Roberta" uniqKey="Gelmini R" first="Roberta" last="Gelmini">Roberta Gelmini</name><affiliation wicri:level="1"><nlm:affiliation>Department of Surgery, AOU Policlinico and University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Surgery, AOU Policlinico and University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author><author><name sortKey="Girardis, Massimo" sort="Girardis, Massimo" uniqKey="Girardis M" first="Massimo" last="Girardis">Massimo Girardis</name><affiliation wicri:level="1"><nlm:affiliation>Department of Anesthesia and Intensive Care Unit, AOU Policlinico and University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Anesthesia and Intensive Care Unit, AOU Policlinico and University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author><author><name sortKey="Guaraldi, Giovanni" sort="Guaraldi, Giovanni" uniqKey="Guaraldi G" first="Giovanni" last="Guaraldi">Giovanni Guaraldi</name><affiliation wicri:level="1"><nlm:affiliation>Infectious Diseases Clinics, AOU Policlinico and University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Infectious Diseases Clinics, AOU Policlinico and University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author><author><name sortKey="Mussini, Cristina" sort="Mussini, Cristina" uniqKey="Mussini C" first="Cristina" last="Mussini">Cristina Mussini</name><affiliation wicri:level="1"><nlm:affiliation>Infectious Diseases Clinics, AOU Policlinico and University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Infectious Diseases Clinics, AOU Policlinico and University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author></analytic><series><title level="j">Cytometry. Part A : the journal of the International Society for Analytical Cytology</title><idno type="eISSN">1552-4930</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The pandemic caused by severe acute respiratory syndrome coronavirus 2 heavily involves all those working in a laboratory. Samples from known infected patients or donors who are considered healthy can arrive, and a colleague might be asymptomatic but able to transmit the virus. Working in a clinical laboratory is posing several safety challenges. Few years ago, International Society for Advancement of Cytometry published guidelines to safely analyze and sort human samples that were revised in these days. We describe the procedures that we have been following since the first patient appeared in Italy, which have only slightly modified our standard one, being all human samples associated with risks. © 2020 International Society for Advancement of Cytometry.</div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">32275124</PMID><DateRevised><Year>2020</Year><Month>04</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1552-4930</ISSN><JournalIssue CitedMedium="Internet"><PubDate><Year>2020</Year><Month>Apr</Month><Day>10</Day></PubDate></JournalIssue><Title>Cytometry. Part A : the journal of the International Society for Analytical Cytology</Title><ISOAbbreviation>Cytometry A</ISOAbbreviation></Journal><ArticleTitle>Handling and Processing of Blood Specimens from Patients with COVID-19 for Safe Studies on Cell Phenotype and Cytokine Storm.</ArticleTitle><ELocationID EIdType="doi" ValidYN="Y">10.1002/cyto.a.24009</ELocationID><Abstract><AbstractText>The pandemic caused by severe acute respiratory syndrome coronavirus 2 heavily involves all those working in a laboratory. Samples from known infected patients or donors who are considered healthy can arrive, and a colleague might be asymptomatic but able to transmit the virus. Working in a clinical laboratory is posing several safety challenges. Few years ago, International Society for Advancement of Cytometry published guidelines to safely analyze and sort human samples that were revised in these days. We describe the procedures that we have been following since the first patient appeared in Italy, which have only slightly modified our standard one, being all human samples associated with risks. © 2020 International Society for Advancement of Cytometry.</AbstractText><CopyrightInformation>© 2020 International Society for Advancement of Cytometry.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Cossarizza</LastName><ForeName>Andrea</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena, Italy.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>National Institute for Cardiovascular Research, Bologna, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gibellini</LastName><ForeName>Lara</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>De Biasi</LastName><ForeName>Sara</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lo Tartaro</LastName><ForeName>Domenico</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mattioli</LastName><ForeName>Marco</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Paolini</LastName><ForeName>Annamaria</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fidanza</LastName><ForeName>Lucia</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bellinazzi</LastName><ForeName>Caterina</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Borella</LastName><ForeName>Rebecca</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Castaniere</LastName><ForeName>Ivana</ForeName><Initials>I</Initials><AffiliationInfo><Affiliation>Respiratory Diseases Unit, AOU Policlinico and University of Modena and Reggio Emilia, Modena, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Meschiari</LastName><ForeName>Marianna</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Infectious Diseases Clinics, AOU Policlinico and University of Modena and Reggio Emilia, Modena, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sita</LastName><ForeName>Marco</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Anesthesia and Intensive Care Unit, AOU Policlinico and University of Modena and Reggio Emilia, Modena, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Manco</LastName><ForeName>Gianrocco</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Department of Surgery, AOU Policlinico and University of Modena and Reggio Emilia, Modena, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Clini</LastName><ForeName>Enrico</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Respiratory Diseases Unit, AOU Policlinico and University of Modena and Reggio Emilia, Modena, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gelmini</LastName><ForeName>Roberta</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Department of Surgery, AOU Policlinico and University of Modena and Reggio Emilia, Modena, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Girardis</LastName><ForeName>Massimo</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Anesthesia and Intensive Care Unit, AOU Policlinico and University of Modena and Reggio Emilia, Modena, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Guaraldi</LastName><ForeName>Giovanni</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Infectious Diseases Clinics, AOU Policlinico and University of Modena and Reggio Emilia, Modena, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mussini</LastName><ForeName>Cristina</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Infectious Diseases Clinics, AOU Policlinico and University of Modena and Reggio Emilia, Modena, Italy.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>04</Month><Day>10</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Cytometry A</MedlineTA><NlmUniqueID>101235694</NlmUniqueID><ISSNLinking>1552-4922</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Covid-19</Keyword><Keyword MajorTopicYN="N">SARS-CoV-2</Keyword><Keyword MajorTopicYN="N">biosafety</Keyword><Keyword MajorTopicYN="N">coronavirus</Keyword><Keyword MajorTopicYN="N">cytokines</Keyword><Keyword MajorTopicYN="N">cytometry</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>03</Month><Day>25</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>03</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>03</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>4</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>4</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>4</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32275124</ArticleId><ArticleId IdType="doi">10.1002/cyto.a.24009</ArticleId></ArticleIdList><ReferenceList><Title>References</Title><Reference><Citation>Zhou P, Yang XL, Wang XG, Hu B, Zhang L, Zhang W, Si HR, Zhu Y, Li B, Huang CL, et al. A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature 2020;579:270-273.</Citation></Reference><Reference><Citation>https://gisanddata.maps.arcgis.com/apps/opsdashboard/index.html#/bda7594740fd40299423467b48e9ecf6</Citation></Reference><Reference><Citation>Cossarizza A, De Biasi S, Guaraldi G, Girardis M, Mussini C. SARS-CoV-2, the virus that causes COVID-19: cytometry and the new challenge for global health. Cytometry A 2020;97:340-343. https://doi.org/10.1002/cyto.a.24002.</Citation></Reference><Reference><Citation>Khan S, Siddique R, Shereen MA, Ali A, Liu J, Bai Q, Bashir N, Xue M. The emergence of a novel coronavirus (SARS-CoV-2), their biology and therapeutic options. J Clin Microbiol 2020 (in press). https://doi.org/10.1128/JCM.00187-20.</Citation></Reference><Reference><Citation>Wang W, Xu Y, Gao R, Lu R, Han K, Wu G, Tan W. Detection of SARS-CoV-2 in different types of clinical specimens. JAMA 2020 (in press). https://doi.org/10.1001/jama.2020.3786.</Citation></Reference><Reference><Citation>Rothan HA, Byrareddy SN. The epidemiology and pathogenesis of coronavirus disease (COVID-19) outbreak. J Autoimmun 2020 (in press);102433. https://doi.org/10.1016/j.jaut.2020.102433.</Citation></Reference><Reference><Citation>Zou L, Ruan F, Huang M, Liang L, Huang H, Hong Z, Yu J, Xia J, Guo Q, Yen H-L, et al. SARS-CoV-2 viral load in upper respiratory specimens of infected patients. N Engl J Med 2020;382:1177-1179.</Citation></Reference><Reference><Citation>van Doremalen N, Bushmaker T, Morris DH, Holbrook MG, Gamble A, Williamson BN, Tamin A, Harcourt JL, Thornburg NJ, Gerber SI, et al. Aerosol and surface stability of SARS-CoV-2 as compared with SARS-CoV-1. N Engl J Med 2020 (in press). https://doi.org/10.1056/NEJMc2004973.</Citation></Reference><Reference><Citation>World Health Organization WHO Laboratory Biosfety Manual, 3rd ed. Geneva, Switzerland: WHO Library; 2004. https://www.who.int/csr/resources/publications/biosafety/Biosafety7.pdf?ua=1</Citation></Reference><Reference><Citation>Schmid I, Lambert C, Ambrozak D, Marti GE, Moss DM, Perfetto SP, International Society of Analytical Cytology. International Society for Analytical Cytology biosafety standard for sorting of unfixed cells. Cytometry A 2007;71:414-437.</Citation></Reference><Reference><Citation>Holmes KL, Fontes B, Hogarth P, Konz R, Monard S, Pletcher CH Jr, Wadley RB, Schmid I, Perfetto SP. International Society for the Advancement of Cytometry cell sorter biosafety standards. Cytometry A 2014;85:434-453.</Citation></Reference><Reference><Citation>Huang C, Wang Y, Li X, Ren L, Zhao J, Hu Y, Zhang L, Fan G, Xu J, Gu X, et al. Clinical features of patients infected with 2019 novel coronavirus in Wuhan. China Lancet 2020;395(10223):497-506.</Citation></Reference><Reference><Citation>Rubio I, Osuchowski MF, Shankar-Hari M, Skirecki T, Winkler MS, Lachmann G, La Rosée P, Monneret G, Venet F, Bauer M, et al. Current gaps in sepsis immunology: New opportunities for translational research. Lancet Infect Dis 2019;e422-e436. https://doi.org/10.1016/S1473-3099(19)30567-5.</Citation></Reference><Reference><Citation>Cossarizza A, Chang HD, Radbruch A, Andrä I, Annunziato F, Bacher P, Barnaba V, Battistini L, Bauer WM, et al. Guidelines for the use of flow cytometry and cell sorting in immunological studies. Eur J Immunol 2017;47:1584-1797.</Citation></Reference><Reference><Citation>Mousset CM, Hobo W, Woestenenk R, Preijers F, Dolstra H, van der Waart AB. Comprehensive phenotyping of T cells using flow cytometry. Cytometry A 2019;95:647-654.</Citation></Reference><Reference><Citation>Roederer M, Nozzi JL, Nason MC. SPICE: exploration and analysis of post-cytometric complex multivariate datasets. Cytometry A 2001;79:167-174.</Citation></Reference><Reference><Citation>Cossarizza A, Cousins D. Overcoming challenges in cellular analysis: Multiparameter analysis of rare cells (Webinar). Science 2015;347(6220):443. https://doi.org/10.1126/science.347.6220.443-c.</Citation></Reference><Reference><Citation>Duvall MG, Precopio ML, Ambrozak DA, Jaye A, McMichael AJ, Whittle HC, Roederer M, Rowland-Jones SL, Koup RA. Polyfunctional T Cell responses are a hallmark of HIV-2 infection. J Exp Med 2008; 38:350-363.</Citation></Reference><Reference><Citation>Cossarizza A, Bertoncelli L, Nemes E, Pinti M, Nasi M, De Biasi S, Gibellini L, Montagna JP, Vecchia M, Manzini L, et al. T cell activation but not polyfunctionality after primary HIV infection predicts control of viral load and length of the time without therapy. PLOS One 2012;7(12):e50728.</Citation></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Italie</li></country></list><tree><country name="Italie"><noRegion><name sortKey="Cossarizza, Andrea" sort="Cossarizza, Andrea" uniqKey="Cossarizza A" first="Andrea" last="Cossarizza">Andrea Cossarizza</name></noRegion><name sortKey="Bellinazzi, Caterina" sort="Bellinazzi, Caterina" uniqKey="Bellinazzi C" first="Caterina" last="Bellinazzi">Caterina Bellinazzi</name><name sortKey="Borella, Rebecca" sort="Borella, Rebecca" uniqKey="Borella R" first="Rebecca" last="Borella">Rebecca Borella</name><name sortKey="Castaniere, Ivana" sort="Castaniere, Ivana" uniqKey="Castaniere I" first="Ivana" last="Castaniere">Ivana Castaniere</name><name sortKey="Clini, Enrico" sort="Clini, Enrico" uniqKey="Clini E" first="Enrico" last="Clini">Enrico Clini</name><name sortKey="De Biasi, Sara" sort="De Biasi, Sara" uniqKey="De Biasi S" first="Sara" last="De Biasi">Sara De Biasi</name><name sortKey="Fidanza, Lucia" sort="Fidanza, Lucia" uniqKey="Fidanza L" first="Lucia" last="Fidanza">Lucia Fidanza</name><name sortKey="Gelmini, Roberta" sort="Gelmini, Roberta" uniqKey="Gelmini R" first="Roberta" last="Gelmini">Roberta Gelmini</name><name sortKey="Gibellini, Lara" sort="Gibellini, Lara" uniqKey="Gibellini L" first="Lara" last="Gibellini">Lara Gibellini</name><name sortKey="Girardis, Massimo" sort="Girardis, Massimo" uniqKey="Girardis M" first="Massimo" last="Girardis">Massimo Girardis</name><name sortKey="Guaraldi, Giovanni" sort="Guaraldi, Giovanni" uniqKey="Guaraldi G" first="Giovanni" last="Guaraldi">Giovanni Guaraldi</name><name sortKey="Lo Tartaro, Domenico" sort="Lo Tartaro, Domenico" uniqKey="Lo Tartaro D" first="Domenico" last="Lo Tartaro">Domenico Lo Tartaro</name><name sortKey="Manco, Gianrocco" sort="Manco, Gianrocco" uniqKey="Manco G" first="Gianrocco" last="Manco">Gianrocco Manco</name><name sortKey="Mattioli, Marco" sort="Mattioli, Marco" uniqKey="Mattioli M" first="Marco" last="Mattioli">Marco Mattioli</name><name sortKey="Meschiari, Marianna" sort="Meschiari, Marianna" uniqKey="Meschiari M" first="Marianna" last="Meschiari">Marianna Meschiari</name><name sortKey="Mussini, Cristina" sort="Mussini, Cristina" uniqKey="Mussini C" first="Cristina" last="Mussini">Cristina Mussini</name><name sortKey="Paolini, Annamaria" sort="Paolini, Annamaria" uniqKey="Paolini A" first="Annamaria" last="Paolini">Annamaria Paolini</name><name sortKey="Sita, Marco" sort="Sita, Marco" uniqKey="Sita M" first="Marco" last="Sita">Marco Sita</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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