Discovery of Bat Coronaviruses through Surveillance and Probe Capture-Based Next-Generation Sequencing
Identifieur interne : 003134 ( Ncbi/Merge ); précédent : 003133; suivant : 003135Discovery of Bat Coronaviruses through Surveillance and Probe Capture-Based Next-Generation Sequencing
Auteurs : Bei Li [République populaire de Chine] ; Hao-Rui Si [République populaire de Chine] ; Yan Zhu [République populaire de Chine] ; Xing-Lou Yang [République populaire de Chine] ; Danielle E. Anderson [Singapour] ; Zheng-Li Shi [République populaire de Chine] ; Lin-Fa Wang [Singapour] ; Peng Zhou [République populaire de Chine]Source :
- mSphere [ 2379-5042 ] ; 2020.
Descripteurs français
- KwdFr :
- MESH :
- isolement et purification : Coronavirus.
- virologie : Chiroptera.
- Animaux, Coronavirus, Génome viral, Séquençage nucléotidique à haut débit, Variation génétique.
English descriptors
- KwdEn :
- MESH :
- classification : Coronavirus.
- isolation & purification : Coronavirus.
- methods : High-Throughput Nucleotide Sequencing.
- virology : Chiroptera.
- Animals, Genetic Variation, Genome, Viral.
Abstract
Active surveillance is both urgent and essential to predict and mitigate the emergence of bat-origin CoV in humans and livestock. However, great genetic diversity increases the chance of homologous recombination among CoVs. Performing targeted PCR, a common practice for many surveillance studies, would not reflect this diversity. NGS, on the other hand, is an expensive methodology and is prone to missing low-abundance CoV sequences. Here, we employ a capture-based NGS approach using baits targeting all CoVs. Our work demonstrates that targeted, cost-effective, large-scale, genome-level surveillance of bat CoVs is now highly feasible.
Url:
DOI: 10.1128/mSphere.00807-19
PubMed: 31996413
PubMed Central: 6992374
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 001640
- to stream Pmc, to step Curation: 001640
- to stream Pmc, to step Checkpoint: 000289
- to stream PubMed, to step Corpus: 000833
- to stream PubMed, to step Curation: 000833
- to stream PubMed, to step Checkpoint: 000564
Links to Exploration step
PMC:6992374Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Discovery of Bat Coronaviruses through Surveillance and Probe Capture-Based Next-Generation Sequencing</title><author><name sortKey="Li, Bei" sort="Li, Bei" uniqKey="Li B" first="Bei" last="Li">Bei Li</name><affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Si, Hao Rui" sort="Si, Hao Rui" uniqKey="Si H" first="Hao-Rui" last="Si">Hao-Rui Si</name><affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation><affiliation wicri:level="3"><nlm:aff id="aff2"><addr-line>University of Chinese Academy of Sciences, Beijing, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>University of Chinese Academy of Sciences, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhu, Yan" sort="Zhu, Yan" uniqKey="Zhu Y" first="Yan" last="Zhu">Yan Zhu</name><affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Yang, Xing Lou" sort="Yang, Xing Lou" uniqKey="Yang X" first="Xing-Lou" last="Yang">Xing-Lou Yang</name><affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Anderson, Danielle E" sort="Anderson, Danielle E" uniqKey="Anderson D" first="Danielle E." last="Anderson">Danielle E. Anderson</name><affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore, Singapore</addr-line></nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore</wicri:regionArea><wicri:noRegion>Singapore</wicri:noRegion></affiliation></author><author><name sortKey="Shi, Zheng Li" sort="Shi, Zheng Li" uniqKey="Shi Z" first="Zheng-Li" last="Shi">Zheng-Li Shi</name><affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Wang, Lin Fa" sort="Wang, Lin Fa" uniqKey="Wang L" first="Lin-Fa" last="Wang">Lin-Fa Wang</name><affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore, Singapore</addr-line></nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore</wicri:regionArea><wicri:noRegion>Singapore</wicri:noRegion></affiliation></author><author><name sortKey="Zhou, Peng" sort="Zhou, Peng" uniqKey="Zhou P" first="Peng" last="Zhou">Peng Zhou</name><affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">31996413</idno><idno type="pmc">6992374</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6992374</idno><idno type="RBID">PMC:6992374</idno><idno type="doi">10.1128/mSphere.00807-19</idno><date when="2020">2020</date><idno type="wicri:Area/Pmc/Corpus">001640</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001640</idno><idno type="wicri:Area/Pmc/Curation">001640</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">001640</idno><idno type="wicri:Area/Pmc/Checkpoint">000289</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000289</idno><idno type="wicri:source">PubMed</idno><idno type="RBID">pubmed:31996413</idno><idno type="wicri:Area/PubMed/Corpus">000833</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000833</idno><idno type="wicri:Area/PubMed/Curation">000833</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000833</idno><idno type="wicri:Area/PubMed/Checkpoint">000564</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000564</idno><idno type="wicri:Area/Ncbi/Merge">003134</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Discovery of Bat Coronaviruses through Surveillance and Probe Capture-Based Next-Generation Sequencing</title><author><name sortKey="Li, Bei" sort="Li, Bei" uniqKey="Li B" first="Bei" last="Li">Bei Li</name><affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Si, Hao Rui" sort="Si, Hao Rui" uniqKey="Si H" first="Hao-Rui" last="Si">Hao-Rui Si</name><affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation><affiliation wicri:level="3"><nlm:aff id="aff2"><addr-line>University of Chinese Academy of Sciences, Beijing, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>University of Chinese Academy of Sciences, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhu, Yan" sort="Zhu, Yan" uniqKey="Zhu Y" first="Yan" last="Zhu">Yan Zhu</name><affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Yang, Xing Lou" sort="Yang, Xing Lou" uniqKey="Yang X" first="Xing-Lou" last="Yang">Xing-Lou Yang</name><affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Anderson, Danielle E" sort="Anderson, Danielle E" uniqKey="Anderson D" first="Danielle E." last="Anderson">Danielle E. Anderson</name><affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore, Singapore</addr-line></nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore</wicri:regionArea><wicri:noRegion>Singapore</wicri:noRegion></affiliation></author><author><name sortKey="Shi, Zheng Li" sort="Shi, Zheng Li" uniqKey="Shi Z" first="Zheng-Li" last="Shi">Zheng-Li Shi</name><affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Wang, Lin Fa" sort="Wang, Lin Fa" uniqKey="Wang L" first="Lin-Fa" last="Wang">Lin-Fa Wang</name><affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore, Singapore</addr-line></nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore</wicri:regionArea><wicri:noRegion>Singapore</wicri:noRegion></affiliation></author><author><name sortKey="Zhou, Peng" sort="Zhou, Peng" uniqKey="Zhou P" first="Peng" last="Zhou">Peng Zhou</name><affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author></analytic><series><title level="j">mSphere</title><idno type="eISSN">2379-5042</idno><imprint><date when="2020">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Chiroptera (virology)</term><term>Coronavirus (classification)</term><term>Coronavirus (isolation & purification)</term><term>Genetic Variation</term><term>Genome, Viral</term><term>High-Throughput Nucleotide Sequencing (methods)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Chiroptera (virologie)</term><term>Coronavirus ()</term><term>Coronavirus (isolement et purification)</term><term>Génome viral</term><term>Séquençage nucléotidique à haut débit ()</term><term>Variation génétique</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>High-Throughput Nucleotide Sequencing</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Chiroptera</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Chiroptera</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Genetic Variation</term><term>Genome, Viral</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Coronavirus</term><term>Génome viral</term><term>Séquençage nucléotidique à haut débit</term><term>Variation génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Active surveillance is both urgent and essential to predict and mitigate the emergence of bat-origin CoV in humans and livestock. However, great genetic diversity increases the chance of homologous recombination among CoVs. Performing targeted PCR, a common practice for many surveillance studies, would not reflect this diversity. NGS, on the other hand, is an expensive methodology and is prone to missing low-abundance CoV sequences. Here, we employ a capture-based NGS approach using baits targeting all CoVs. Our work demonstrates that targeted, cost-effective, large-scale, genome-level surveillance of bat CoVs is now highly feasible.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Forni, D" uniqKey="Forni D">D Forni</name></author><author><name sortKey="Cagliani, R" uniqKey="Cagliani R">R Cagliani</name></author><author><name sortKey="Clerici, M" uniqKey="Clerici M">M Clerici</name></author><author><name sortKey="Sironi, M" uniqKey="Sironi M">M Sironi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Woo, Pc" uniqKey="Woo P">PC Woo</name></author><author><name sortKey="Lau, Sk" uniqKey="Lau S">SK Lau</name></author><author><name sortKey="Huang, Y" uniqKey="Huang Y">Y Huang</name></author><author><name sortKey="Yuen, Ky" uniqKey="Yuen K">KY Yuen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lau, Sk" uniqKey="Lau S">SK Lau</name></author><author><name sortKey="Woo, Pc" uniqKey="Woo P">PC Woo</name></author><author><name sortKey="Yip, Cc" uniqKey="Yip C">CC Yip</name></author><author><name sortKey="Tse, H" uniqKey="Tse H">H Tse</name></author><author><name sortKey="Tsoi, Hw" uniqKey="Tsoi H">HW Tsoi</name></author><author><name sortKey="Cheng, Vc" uniqKey="Cheng V">VC Cheng</name></author><author><name sortKey="Lee, P" uniqKey="Lee P">P Lee</name></author><author><name sortKey="Tang, Bs" uniqKey="Tang B">BS Tang</name></author><author><name sortKey="Cheung, Ch" uniqKey="Cheung C">CH Cheung</name></author><author><name sortKey="Lee, Ra" uniqKey="Lee R">RA Lee</name></author><author><name sortKey="So, Ly" uniqKey="So L">LY So</name></author><author><name sortKey="Lau, Yl" uniqKey="Lau Y">YL Lau</name></author><author><name sortKey="Chan, Kh" uniqKey="Chan K">KH Chan</name></author><author><name sortKey="Yuen, Ky" uniqKey="Yuen K">KY Yuen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Su, S" uniqKey="Su S">S Su</name></author><author><name sortKey="Wong, G" uniqKey="Wong G">G Wong</name></author><author><name sortKey="Shi, W" uniqKey="Shi W">W Shi</name></author><author><name sortKey="Liu, J" uniqKey="Liu J">J Liu</name></author><author><name sortKey="Lai, Ack" uniqKey="Lai A">ACK Lai</name></author><author><name sortKey="Zhou, J" uniqKey="Zhou J">J Zhou</name></author><author><name sortKey="Liu, W" uniqKey="Liu W">W Liu</name></author><author><name sortKey="Bi, Y" uniqKey="Bi Y">Y Bi</name></author><author><name sortKey="Gao, Gf" uniqKey="Gao G">GF Gao</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Woo, Pc" uniqKey="Woo P">PC Woo</name></author><author><name sortKey="Huang, Y" uniqKey="Huang Y">Y Huang</name></author><author><name sortKey="Lau, Sk" uniqKey="Lau S">SK Lau</name></author><author><name sortKey="Yuen, Ky" uniqKey="Yuen K">KY Yuen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hu, B" uniqKey="Hu B">B Hu</name></author><author><name sortKey="Zeng, Lp" uniqKey="Zeng L">LP Zeng</name></author><author><name sortKey="Yang, Xl" uniqKey="Yang X">XL Yang</name></author><author><name sortKey="Ge, Xy" uniqKey="Ge X">XY Ge</name></author><author><name sortKey="Zhang, W" uniqKey="Zhang W">W Zhang</name></author><author><name sortKey="Li, B" uniqKey="Li B">B Li</name></author><author><name sortKey="Xie, Jz" uniqKey="Xie J">JZ Xie</name></author><author><name sortKey="Shen, Xr" uniqKey="Shen X">XR Shen</name></author><author><name sortKey="Zhang, Yz" uniqKey="Zhang Y">YZ Zhang</name></author><author><name sortKey="Wang, N" uniqKey="Wang N">N Wang</name></author><author><name sortKey="Luo, Ds" uniqKey="Luo D">DS Luo</name></author><author><name sortKey="Zheng, Xs" uniqKey="Zheng X">XS Zheng</name></author><author><name sortKey="Wang, Mn" uniqKey="Wang M">MN Wang</name></author><author><name sortKey="Daszak, P" uniqKey="Daszak P">P Daszak</name></author><author><name sortKey="Wang, Lf" uniqKey="Wang L">LF Wang</name></author><author><name sortKey="Cui, J" uniqKey="Cui J">J Cui</name></author><author><name sortKey="Shi, Zl" uniqKey="Shi Z">ZL Shi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="De Souza Luna, Lk" uniqKey="De Souza Luna L">LK de Souza Luna</name></author><author><name sortKey="Heiser, V" uniqKey="Heiser V">V Heiser</name></author><author><name sortKey="Regamey, N" uniqKey="Regamey N">N Regamey</name></author><author><name sortKey="Panning, M" uniqKey="Panning M">M Panning</name></author><author><name sortKey="Drexler, Jf" uniqKey="Drexler J">JF Drexler</name></author><author><name sortKey="Mulangu, S" uniqKey="Mulangu S">S Mulangu</name></author><author><name sortKey="Poon, L" uniqKey="Poon L">L Poon</name></author><author><name sortKey="Baumgarte, S" uniqKey="Baumgarte S">S Baumgarte</name></author><author><name sortKey="Haijema, Bj" uniqKey="Haijema B">BJ Haijema</name></author><author><name sortKey="Kaiser, L" uniqKey="Kaiser L">L Kaiser</name></author><author><name sortKey="Drosten, C" uniqKey="Drosten C">C Drosten</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Drosten, C" uniqKey="Drosten C">C Drosten</name></author><author><name sortKey="Gunther, S" uniqKey="Gunther S">S Gunther</name></author><author><name sortKey="Preiser, W" uniqKey="Preiser W">W Preiser</name></author><author><name sortKey="Van Der Werf, S" uniqKey="Van Der Werf S">S van der Werf</name></author><author><name sortKey="Brodt, Hr" uniqKey="Brodt H">HR Brodt</name></author><author><name sortKey="Becker, S" uniqKey="Becker S">S Becker</name></author><author><name sortKey="Rabenau, H" uniqKey="Rabenau H">H Rabenau</name></author><author><name sortKey="Panning, M" uniqKey="Panning M">M Panning</name></author><author><name sortKey="Kolesnikova, L" uniqKey="Kolesnikova L">L Kolesnikova</name></author><author><name sortKey="Fouchier, Ram" uniqKey="Fouchier R">RAM Fouchier</name></author><author><name sortKey="Berger, A" uniqKey="Berger A">A Berger</name></author><author><name sortKey="Burguiere, Am" uniqKey="Burguiere A">AM Burguiere</name></author><author><name sortKey="Cinatl, J" uniqKey="Cinatl J">J Cinatl</name></author><author><name sortKey="Eickmann, M" uniqKey="Eickmann M">M Eickmann</name></author><author><name sortKey="Escriou, N" uniqKey="Escriou N">N Escriou</name></author><author><name sortKey="Grywna, K" uniqKey="Grywna K">K Grywna</name></author><author><name sortKey="Kramme, S" uniqKey="Kramme S">S Kramme</name></author><author><name sortKey="Manuguerra, Jc" uniqKey="Manuguerra J">JC Manuguerra</name></author><author><name sortKey="Muller, S" uniqKey="Muller S">S Muller</name></author><author><name sortKey="Rickerts, V" uniqKey="Rickerts V">V Rickerts</name></author><author><name sortKey="Sturmer, M" uniqKey="Sturmer M">M Sturmer</name></author><author><name sortKey="Vieth, S" uniqKey="Vieth S">S Vieth</name></author><author><name sortKey="Klenk, Hd" uniqKey="Klenk H">HD Klenk</name></author><author><name sortKey="Osterhaus, A" uniqKey="Osterhaus A">A Osterhaus</name></author><author><name sortKey="Schmitz, H" uniqKey="Schmitz H">H Schmitz</name></author><author><name sortKey="Doerr, Hw" uniqKey="Doerr H">HW Doerr</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zaki, Am" uniqKey="Zaki A">AM Zaki</name></author><author><name sortKey="Van Boheemen, S" uniqKey="Van Boheemen S">S van Boheemen</name></author><author><name sortKey="Bestebroer, Tm" uniqKey="Bestebroer T">TM Bestebroer</name></author><author><name sortKey="Osterhaus, A" uniqKey="Osterhaus A">A Osterhaus</name></author><author><name sortKey="Fouchier, R" uniqKey="Fouchier R">R Fouchier</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhou, P" uniqKey="Zhou P">P Zhou</name></author><author><name sortKey="Fan, H" uniqKey="Fan H">H Fan</name></author><author><name sortKey="Lan, T" uniqKey="Lan T">T Lan</name></author><author><name sortKey="Yang, Xl" uniqKey="Yang X">XL Yang</name></author><author><name sortKey="Shi, Wf" uniqKey="Shi W">WF Shi</name></author><author><name sortKey="Zhang, W" uniqKey="Zhang W">W Zhang</name></author><author><name sortKey="Zhu, Y" uniqKey="Zhu Y">Y Zhu</name></author><author><name sortKey="Zhang, Yw" uniqKey="Zhang Y">YW Zhang</name></author><author><name sortKey="Xie, Qm" uniqKey="Xie Q">QM Xie</name></author><author><name sortKey="Mani, S" uniqKey="Mani S">S Mani</name></author><author><name sortKey="Zheng, Xs" uniqKey="Zheng X">XS Zheng</name></author><author><name sortKey="Li, B" uniqKey="Li B">B Li</name></author><author><name sortKey="Li, Jm" uniqKey="Li J">JM Li</name></author><author><name sortKey="Guo, H" uniqKey="Guo H">H Guo</name></author><author><name sortKey="Pei, Gq" uniqKey="Pei G">GQ Pei</name></author><author><name sortKey="An, Xp" uniqKey="An X">XP An</name></author><author><name sortKey="Chen, Jw" uniqKey="Chen J">JW Chen</name></author><author><name sortKey="Zhou, L" uniqKey="Zhou L">L Zhou</name></author><author><name sortKey="Mai, Kj" uniqKey="Mai K">KJ Mai</name></author><author><name sortKey="Wu, Zx" uniqKey="Wu Z">ZX Wu</name></author><author><name sortKey="Li, D" uniqKey="Li D">D Li</name></author><author><name sortKey="Anderson, De" uniqKey="Anderson D">DE Anderson</name></author><author><name sortKey="Zhang, Lb" uniqKey="Zhang L">LB Zhang</name></author><author><name sortKey="Li, Sy" uniqKey="Li S">SY Li</name></author><author><name sortKey="Mi, Zq" uniqKey="Mi Z">ZQ Mi</name></author><author><name sortKey="He, Tt" uniqKey="He T">TT He</name></author><author><name sortKey="Cong, F" uniqKey="Cong F">F Cong</name></author><author><name sortKey="Guo, Pj" uniqKey="Guo P">PJ Guo</name></author><author><name sortKey="Huang, R" uniqKey="Huang R">R Huang</name></author><author><name sortKey="Luo, Y" uniqKey="Luo Y">Y Luo</name></author><author><name sortKey="Liu, Xl" uniqKey="Liu X">XL Liu</name></author><author><name sortKey="Chen, J" uniqKey="Chen J">J Chen</name></author><author><name sortKey="Huang, Y" uniqKey="Huang Y">Y Huang</name></author><author><name sortKey="Sun, Q" uniqKey="Sun Q">Q Sun</name></author><author><name sortKey="Zhang, Xll" uniqKey="Zhang X">XLL Zhang</name></author><author><name sortKey="Wang, Yy" uniqKey="Wang Y">YY Wang</name></author><author><name sortKey="Xing, Sz" uniqKey="Xing S">SZ Xing</name></author><author><name sortKey="Chen, Ys" uniqKey="Chen Y">YS Chen</name></author><author><name sortKey="Sun, Y" uniqKey="Sun Y">Y Sun</name></author><author><name sortKey="Li, J" uniqKey="Li J">J Li</name></author><author><name sortKey="Daszak, P" uniqKey="Daszak P">P Daszak</name></author><author><name sortKey="Wang, Lf" uniqKey="Wang L">LF Wang</name></author><author><name sortKey="Shi, Zl" uniqKey="Shi Z">ZL Shi</name></author><author><name sortKey="Tong, Yg" uniqKey="Tong Y">YG Tong</name></author><author><name sortKey="Ma, Jy" uniqKey="Ma J">JY Ma</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, L F" uniqKey="Wang L">L-F Wang</name></author><author><name sortKey="Cowled, C" uniqKey="Cowled C">C Cowled</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fan, Y" uniqKey="Fan Y">Y Fan</name></author><author><name sortKey="Zhao, K" uniqKey="Zhao K">K Zhao</name></author><author><name sortKey="Shi, Zl" uniqKey="Shi Z">ZL Shi</name></author><author><name sortKey="Zhou, P" uniqKey="Zhou P">P Zhou</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Carroll, D" uniqKey="Carroll D">D Carroll</name></author><author><name sortKey="Daszak, P" uniqKey="Daszak P">P Daszak</name></author><author><name sortKey="Wolfe, Nd" uniqKey="Wolfe N">ND Wolfe</name></author><author><name sortKey="Gao, Gf" uniqKey="Gao G">GF Gao</name></author><author><name sortKey="Morel, Cm" uniqKey="Morel C">CM Morel</name></author><author><name sortKey="Morzaria, S" uniqKey="Morzaria S">S Morzaria</name></author><author><name sortKey="Pablos Mendez, A" uniqKey="Pablos Mendez A">A Pablos-Mendez</name></author><author><name sortKey="Tomori, O" uniqKey="Tomori O">O Tomori</name></author><author><name sortKey="Mazet, J" uniqKey="Mazet J">J Mazet</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Briese, T" uniqKey="Briese T">T Briese</name></author><author><name sortKey="Kapoor, A" uniqKey="Kapoor A">A Kapoor</name></author><author><name sortKey="Mishra, N" uniqKey="Mishra N">N Mishra</name></author><author><name sortKey="Jain, K" uniqKey="Jain K">K Jain</name></author><author><name sortKey="Kumar, A" uniqKey="Kumar A">A Kumar</name></author><author><name sortKey="Jabado, Oj" uniqKey="Jabado O">OJ Jabado</name></author><author><name sortKey="Lipkin, Wi" uniqKey="Lipkin W">WI Lipkin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gaudin, M" uniqKey="Gaudin M">M Gaudin</name></author><author><name sortKey="Desnues, C" uniqKey="Desnues C">C Desnues</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wylie, Tn" uniqKey="Wylie T">TN Wylie</name></author><author><name sortKey="Wylie, Km" uniqKey="Wylie K">KM Wylie</name></author><author><name sortKey="Herter, Bn" uniqKey="Herter B">BN Herter</name></author><author><name sortKey="Storch, Ga" uniqKey="Storch G">GA Storch</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lim, Xf" uniqKey="Lim X">XF Lim</name></author><author><name sortKey="Lee, Cb" uniqKey="Lee C">CB Lee</name></author><author><name sortKey="Pascoe, Sm" uniqKey="Pascoe S">SM Pascoe</name></author><author><name sortKey="How, Cb" uniqKey="How C">CB How</name></author><author><name sortKey="Chan, S" uniqKey="Chan S">S Chan</name></author><author><name sortKey="Tan, Jh" uniqKey="Tan J">JH Tan</name></author><author><name sortKey="Yang, X" uniqKey="Yang X">X Yang</name></author><author><name sortKey="Zhou, P" uniqKey="Zhou P">P Zhou</name></author><author><name sortKey="Shi, Z" uniqKey="Shi Z">Z Shi</name></author><author><name sortKey="Sessions, Om" uniqKey="Sessions O">OM Sessions</name></author><author><name sortKey="Wang, Lf" uniqKey="Wang L">LF Wang</name></author><author><name sortKey="Ng, Lc" uniqKey="Ng L">LC Ng</name></author><author><name sortKey="Anderson, De" uniqKey="Anderson D">DE Anderson</name></author><author><name sortKey="Yap, G" uniqKey="Yap G">G Yap</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Noyes, Nr" uniqKey="Noyes N">NR Noyes</name></author><author><name sortKey="Weinroth, Me" uniqKey="Weinroth M">ME Weinroth</name></author><author><name sortKey="Parker, Jk" uniqKey="Parker J">JK Parker</name></author><author><name sortKey="Dean, Cj" uniqKey="Dean C">CJ Dean</name></author><author><name sortKey="Lakin, Sm" uniqKey="Lakin S">SM Lakin</name></author><author><name sortKey="Raymond, Ra" uniqKey="Raymond R">RA Raymond</name></author><author><name sortKey="Rovira, P" uniqKey="Rovira P">P Rovira</name></author><author><name sortKey="Doster, E" uniqKey="Doster E">E Doster</name></author><author><name sortKey="Abdo, Z" uniqKey="Abdo Z">Z Abdo</name></author><author><name sortKey="Martin, Jn" uniqKey="Martin J">JN Martin</name></author><author><name sortKey="Jones, Kl" uniqKey="Jones K">KL Jones</name></author><author><name sortKey="Ruiz, J" uniqKey="Ruiz J">J Ruiz</name></author><author><name sortKey="Boucher, Ca" uniqKey="Boucher C">CA Boucher</name></author><author><name sortKey="Belk, Ke" uniqKey="Belk K">KE Belk</name></author><author><name sortKey="Morley, Ps" uniqKey="Morley P">PS Morley</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Metsky, Hc" uniqKey="Metsky H">HC Metsky</name></author><author><name sortKey="Siddle, Kj" uniqKey="Siddle K">KJ Siddle</name></author><author><name sortKey="Gladden Young, A" uniqKey="Gladden Young A">A Gladden-Young</name></author><author><name sortKey="Qu, J" uniqKey="Qu J">J Qu</name></author><author><name sortKey="Yang, Dk" uniqKey="Yang D">DK Yang</name></author><author><name sortKey="Brehio, P" uniqKey="Brehio P">P Brehio</name></author><author><name sortKey="Goldfarb, A" uniqKey="Goldfarb A">A Goldfarb</name></author><author><name sortKey="Piantadosi, A" uniqKey="Piantadosi A">A Piantadosi</name></author><author><name sortKey="Wohl, S" uniqKey="Wohl S">S Wohl</name></author><author><name sortKey="Carter, A" uniqKey="Carter A">A Carter</name></author><author><name sortKey="Lin, Ae" uniqKey="Lin A">AE Lin</name></author><author><name sortKey="Barnes, Kg" uniqKey="Barnes K">KG Barnes</name></author><author><name sortKey="Tully, Dc" uniqKey="Tully D">DC Tully</name></author><author><name sortKey="Corleis, B" uniqKey="Corleis B">B Corleis</name></author><author><name sortKey="Hennigan, S" uniqKey="Hennigan S">S Hennigan</name></author><author><name sortKey="Barbosa Lima, G" uniqKey="Barbosa Lima G">G Barbosa-Lima</name></author><author><name sortKey="Vieira, Yr" uniqKey="Vieira Y">YR Vieira</name></author><author><name sortKey="Paul, Lm" uniqKey="Paul L">LM Paul</name></author><author><name sortKey="Tan, Al" uniqKey="Tan A">AL Tan</name></author><author><name sortKey="Garcia, Kf" uniqKey="Garcia K">KF Garcia</name></author><author><name sortKey="Parham, La" uniqKey="Parham L">LA Parham</name></author><author><name sortKey="Odia, I" uniqKey="Odia I">I Odia</name></author><author><name sortKey="Eromon, P" uniqKey="Eromon P">P Eromon</name></author><author><name sortKey="Folarin, Oa" uniqKey="Folarin O">OA Folarin</name></author><author><name sortKey="Goba, A" uniqKey="Goba A">A Goba</name></author><author><name sortKey="Simon Loriere, E" uniqKey="Simon Loriere E">E Simon-Loriere</name></author><author><name sortKey="Hensley, L" uniqKey="Hensley L">L Hensley</name></author><author><name sortKey="Balmaseda, A" uniqKey="Balmaseda A">A Balmaseda</name></author><author><name sortKey="Harris, E" uniqKey="Harris E">E Harris</name></author><author><name sortKey="Kwon, Ds" uniqKey="Kwon D">DS Kwon</name></author><author><name sortKey="Allen, Tm" uniqKey="Allen T">TM Allen</name></author><author><name sortKey="Runstadler, Ja" uniqKey="Runstadler J">JA Runstadler</name></author><author><name sortKey="Smole, S" uniqKey="Smole S">S Smole</name></author><author><name sortKey="Bozza, Fa" uniqKey="Bozza F">FA Bozza</name></author><author><name sortKey="Souza, Tml" uniqKey="Souza T">TML Souza</name></author><author><name sortKey="Isern, S" uniqKey="Isern S">S Isern</name></author><author><name sortKey="Michael, Sf" uniqKey="Michael S">SF Michael</name></author><author><name sortKey="Lorenzana, I" uniqKey="Lorenzana I">I Lorenzana</name></author><author><name sortKey="Gehrke, L" uniqKey="Gehrke L">L Gehrke</name></author><author><name sortKey="Bosch, I" uniqKey="Bosch I">I Bosch</name></author><author><name sortKey="Ebel, G" uniqKey="Ebel G">G Ebel</name></author><author><name sortKey="Grant, Ds" uniqKey="Grant D">DS Grant</name></author><author><name sortKey="Happi, Ct" uniqKey="Happi C">CT Happi</name></author><author><name sortKey="Park, Dj" uniqKey="Park D">DJ Park</name></author><author><name sortKey="Gnirke, A" uniqKey="Gnirke A">A Gnirke</name></author><author><name sortKey="Sabeti, Pc" uniqKey="Sabeti P">PC Sabeti</name></author><author><name sortKey="Matranga, Cb" uniqKey="Matranga C">CB Matranga</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lau, Sk" uniqKey="Lau S">SK Lau</name></author><author><name sortKey="Woo, Pc" uniqKey="Woo P">PC Woo</name></author><author><name sortKey="Li, Ks" uniqKey="Li K">KS Li</name></author><author><name sortKey="Huang, Y" uniqKey="Huang Y">Y Huang</name></author><author><name sortKey="Wang, M" uniqKey="Wang M">M Wang</name></author><author><name sortKey="Lam, Cs" uniqKey="Lam C">CS Lam</name></author><author><name sortKey="Xu, H" uniqKey="Xu H">H Xu</name></author><author><name sortKey="Guo, R" uniqKey="Guo R">R Guo</name></author><author><name sortKey="Chan, Kh" uniqKey="Chan K">KH Chan</name></author><author><name sortKey="Zheng, Bj" uniqKey="Zheng B">BJ Zheng</name></author><author><name sortKey="Yuen, Ky" uniqKey="Yuen K">KY Yuen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, W" uniqKey="Zhang W">W Zhang</name></author><author><name sortKey="Zheng, Xs" uniqKey="Zheng X">XS Zheng</name></author><author><name sortKey="Agwanda, B" uniqKey="Agwanda B">B Agwanda</name></author><author><name sortKey="Ommeh, S" uniqKey="Ommeh S">S Ommeh</name></author><author><name sortKey="Zhao, K" uniqKey="Zhao K">K Zhao</name></author><author><name sortKey="Lichoti, J" uniqKey="Lichoti J">J Lichoti</name></author><author><name sortKey="Wang, N" uniqKey="Wang N">N Wang</name></author><author><name sortKey="Chen, J" uniqKey="Chen J">J Chen</name></author><author><name sortKey="Li, B" uniqKey="Li B">B Li</name></author><author><name sortKey="Yang, Xl" uniqKey="Yang X">XL Yang</name></author><author><name sortKey="Mani, S" uniqKey="Mani S">S Mani</name></author><author><name sortKey="Ngeiywa, Kj" uniqKey="Ngeiywa K">KJ Ngeiywa</name></author><author><name sortKey="Zhu, Y" uniqKey="Zhu Y">Y Zhu</name></author><author><name sortKey="Hu, B" uniqKey="Hu B">B Hu</name></author><author><name sortKey="Onyuok, So" uniqKey="Onyuok S">SO Onyuok</name></author><author><name sortKey="Yan, B" uniqKey="Yan B">B Yan</name></author><author><name sortKey="Anderson, De" uniqKey="Anderson D">DE Anderson</name></author><author><name sortKey="Wang, Lf" uniqKey="Wang L">LF Wang</name></author><author><name sortKey="Zhou, P" uniqKey="Zhou P">P Zhou</name></author><author><name sortKey="Shi, Zl" uniqKey="Shi Z">ZL Shi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Luo, C M" uniqKey="Luo C">C-M Luo</name></author><author><name sortKey="Wang, N" uniqKey="Wang N">N Wang</name></author><author><name sortKey="Yang, X L" uniqKey="Yang X">X-L Yang</name></author><author><name sortKey="Liu, H Z" uniqKey="Liu H">H-Z Liu</name></author><author><name sortKey="Zhang, W" uniqKey="Zhang W">W Zhang</name></author><author><name sortKey="Li, B" uniqKey="Li B">B Li</name></author><author><name sortKey="Hu, B" uniqKey="Hu B">B Hu</name></author><author><name sortKey="Peng, C" uniqKey="Peng C">C Peng</name></author><author><name sortKey="Geng, Q B" uniqKey="Geng Q">Q-B Geng</name></author><author><name sortKey="Zhu, G J" uniqKey="Zhu G">G-J Zhu</name></author><author><name sortKey="Li, F" uniqKey="Li F">F Li</name></author><author><name sortKey="Shi, Z L" uniqKey="Shi Z">Z-L Shi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ge, Xy" uniqKey="Ge X">XY Ge</name></author><author><name sortKey="Wang, N" uniqKey="Wang N">N Wang</name></author><author><name sortKey="Zhang, W" uniqKey="Zhang W">W Zhang</name></author><author><name sortKey="Hu, B" uniqKey="Hu B">B Hu</name></author><author><name sortKey="Li, B" uniqKey="Li B">B Li</name></author><author><name sortKey="Zhang, Yz" uniqKey="Zhang Y">YZ Zhang</name></author><author><name sortKey="Zhou, Jh" uniqKey="Zhou J">JH Zhou</name></author><author><name sortKey="Luo, Cm" uniqKey="Luo C">CM Luo</name></author><author><name sortKey="Yang, Xl" uniqKey="Yang X">XL Yang</name></author><author><name sortKey="Wu, Lj" uniqKey="Wu L">LJ Wu</name></author><author><name sortKey="Wang, B" uniqKey="Wang B">B Wang</name></author><author><name sortKey="Zhang, Y" uniqKey="Zhang Y">Y Zhang</name></author><author><name sortKey="Li, Zx" uniqKey="Li Z">ZX Li</name></author><author><name sortKey="Shi, Zl" uniqKey="Shi Z">ZL Shi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Goecks, J" uniqKey="Goecks J">J Goecks</name></author><author><name sortKey="Nekrutenko, A" uniqKey="Nekrutenko A">A Nekrutenko</name></author><author><name sortKey="Taylor, J" uniqKey="Taylor J">J Taylor</name></author></analytic></biblStruct></listBibl></div1></back></TEI><double pmid="31996413"><pmc><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Discovery of Bat Coronaviruses through Surveillance and Probe Capture-Based Next-Generation Sequencing</title><author><name sortKey="Li, Bei" sort="Li, Bei" uniqKey="Li B" first="Bei" last="Li">Bei Li</name><affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Si, Hao Rui" sort="Si, Hao Rui" uniqKey="Si H" first="Hao-Rui" last="Si">Hao-Rui Si</name><affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation><affiliation wicri:level="3"><nlm:aff id="aff2"><addr-line>University of Chinese Academy of Sciences, Beijing, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>University of Chinese Academy of Sciences, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhu, Yan" sort="Zhu, Yan" uniqKey="Zhu Y" first="Yan" last="Zhu">Yan Zhu</name><affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Yang, Xing Lou" sort="Yang, Xing Lou" uniqKey="Yang X" first="Xing-Lou" last="Yang">Xing-Lou Yang</name><affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Anderson, Danielle E" sort="Anderson, Danielle E" uniqKey="Anderson D" first="Danielle E." last="Anderson">Danielle E. Anderson</name><affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore, Singapore</addr-line></nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore</wicri:regionArea><wicri:noRegion>Singapore</wicri:noRegion></affiliation></author><author><name sortKey="Shi, Zheng Li" sort="Shi, Zheng Li" uniqKey="Shi Z" first="Zheng-Li" last="Shi">Zheng-Li Shi</name><affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Wang, Lin Fa" sort="Wang, Lin Fa" uniqKey="Wang L" first="Lin-Fa" last="Wang">Lin-Fa Wang</name><affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore, Singapore</addr-line></nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore</wicri:regionArea><wicri:noRegion>Singapore</wicri:noRegion></affiliation></author><author><name sortKey="Zhou, Peng" sort="Zhou, Peng" uniqKey="Zhou P" first="Peng" last="Zhou">Peng Zhou</name><affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">31996413</idno><idno type="pmc">6992374</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6992374</idno><idno type="RBID">PMC:6992374</idno><idno type="doi">10.1128/mSphere.00807-19</idno><date when="2020">2020</date><idno type="wicri:Area/Pmc/Corpus">001640</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001640</idno><idno type="wicri:Area/Pmc/Curation">001640</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">001640</idno><idno type="wicri:Area/Pmc/Checkpoint">000289</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000289</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Discovery of Bat Coronaviruses through Surveillance and Probe Capture-Based Next-Generation Sequencing</title><author><name sortKey="Li, Bei" sort="Li, Bei" uniqKey="Li B" first="Bei" last="Li">Bei Li</name><affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Si, Hao Rui" sort="Si, Hao Rui" uniqKey="Si H" first="Hao-Rui" last="Si">Hao-Rui Si</name><affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation><affiliation wicri:level="3"><nlm:aff id="aff2"><addr-line>University of Chinese Academy of Sciences, Beijing, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>University of Chinese Academy of Sciences, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhu, Yan" sort="Zhu, Yan" uniqKey="Zhu Y" first="Yan" last="Zhu">Yan Zhu</name><affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Yang, Xing Lou" sort="Yang, Xing Lou" uniqKey="Yang X" first="Xing-Lou" last="Yang">Xing-Lou Yang</name><affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Anderson, Danielle E" sort="Anderson, Danielle E" uniqKey="Anderson D" first="Danielle E." last="Anderson">Danielle E. Anderson</name><affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore, Singapore</addr-line></nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore</wicri:regionArea><wicri:noRegion>Singapore</wicri:noRegion></affiliation></author><author><name sortKey="Shi, Zheng Li" sort="Shi, Zheng Li" uniqKey="Shi Z" first="Zheng-Li" last="Shi">Zheng-Li Shi</name><affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Wang, Lin Fa" sort="Wang, Lin Fa" uniqKey="Wang L" first="Lin-Fa" last="Wang">Lin-Fa Wang</name><affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore, Singapore</addr-line></nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore</wicri:regionArea><wicri:noRegion>Singapore</wicri:noRegion></affiliation></author><author><name sortKey="Zhou, Peng" sort="Zhou, Peng" uniqKey="Zhou P" first="Peng" last="Zhou">Peng Zhou</name><affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author></analytic><series><title level="j">mSphere</title><idno type="eISSN">2379-5042</idno><imprint><date when="2020">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Active surveillance is both urgent and essential to predict and mitigate the emergence of bat-origin CoV in humans and livestock. However, great genetic diversity increases the chance of homologous recombination among CoVs. Performing targeted PCR, a common practice for many surveillance studies, would not reflect this diversity. NGS, on the other hand, is an expensive methodology and is prone to missing low-abundance CoV sequences. Here, we employ a capture-based NGS approach using baits targeting all CoVs. Our work demonstrates that targeted, cost-effective, large-scale, genome-level surveillance of bat CoVs is now highly feasible.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Forni, D" uniqKey="Forni D">D Forni</name></author><author><name sortKey="Cagliani, R" uniqKey="Cagliani R">R Cagliani</name></author><author><name sortKey="Clerici, M" uniqKey="Clerici M">M Clerici</name></author><author><name sortKey="Sironi, M" uniqKey="Sironi M">M Sironi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Woo, Pc" uniqKey="Woo P">PC Woo</name></author><author><name sortKey="Lau, Sk" uniqKey="Lau S">SK Lau</name></author><author><name sortKey="Huang, Y" uniqKey="Huang Y">Y Huang</name></author><author><name sortKey="Yuen, Ky" uniqKey="Yuen K">KY Yuen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lau, Sk" uniqKey="Lau S">SK Lau</name></author><author><name sortKey="Woo, Pc" uniqKey="Woo P">PC Woo</name></author><author><name sortKey="Yip, Cc" uniqKey="Yip C">CC Yip</name></author><author><name sortKey="Tse, H" uniqKey="Tse H">H Tse</name></author><author><name sortKey="Tsoi, Hw" uniqKey="Tsoi H">HW Tsoi</name></author><author><name sortKey="Cheng, Vc" uniqKey="Cheng V">VC Cheng</name></author><author><name sortKey="Lee, P" uniqKey="Lee P">P Lee</name></author><author><name sortKey="Tang, Bs" uniqKey="Tang B">BS Tang</name></author><author><name sortKey="Cheung, Ch" uniqKey="Cheung C">CH Cheung</name></author><author><name sortKey="Lee, Ra" uniqKey="Lee R">RA Lee</name></author><author><name sortKey="So, Ly" uniqKey="So L">LY So</name></author><author><name sortKey="Lau, Yl" uniqKey="Lau Y">YL Lau</name></author><author><name sortKey="Chan, Kh" uniqKey="Chan K">KH Chan</name></author><author><name sortKey="Yuen, Ky" uniqKey="Yuen K">KY Yuen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Su, S" uniqKey="Su S">S Su</name></author><author><name sortKey="Wong, G" uniqKey="Wong G">G Wong</name></author><author><name sortKey="Shi, W" uniqKey="Shi W">W Shi</name></author><author><name sortKey="Liu, J" uniqKey="Liu J">J Liu</name></author><author><name sortKey="Lai, Ack" uniqKey="Lai A">ACK Lai</name></author><author><name sortKey="Zhou, J" uniqKey="Zhou J">J Zhou</name></author><author><name sortKey="Liu, W" uniqKey="Liu W">W Liu</name></author><author><name sortKey="Bi, Y" uniqKey="Bi Y">Y Bi</name></author><author><name sortKey="Gao, Gf" uniqKey="Gao G">GF Gao</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Woo, Pc" uniqKey="Woo P">PC Woo</name></author><author><name sortKey="Huang, Y" uniqKey="Huang Y">Y Huang</name></author><author><name sortKey="Lau, Sk" uniqKey="Lau S">SK Lau</name></author><author><name sortKey="Yuen, Ky" uniqKey="Yuen K">KY Yuen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hu, B" uniqKey="Hu B">B Hu</name></author><author><name sortKey="Zeng, Lp" uniqKey="Zeng L">LP Zeng</name></author><author><name sortKey="Yang, Xl" uniqKey="Yang X">XL Yang</name></author><author><name sortKey="Ge, Xy" uniqKey="Ge X">XY Ge</name></author><author><name sortKey="Zhang, W" uniqKey="Zhang W">W Zhang</name></author><author><name sortKey="Li, B" uniqKey="Li B">B Li</name></author><author><name sortKey="Xie, Jz" uniqKey="Xie J">JZ Xie</name></author><author><name sortKey="Shen, Xr" uniqKey="Shen X">XR Shen</name></author><author><name sortKey="Zhang, Yz" uniqKey="Zhang Y">YZ Zhang</name></author><author><name sortKey="Wang, N" uniqKey="Wang N">N Wang</name></author><author><name sortKey="Luo, Ds" uniqKey="Luo D">DS Luo</name></author><author><name sortKey="Zheng, Xs" uniqKey="Zheng X">XS Zheng</name></author><author><name sortKey="Wang, Mn" uniqKey="Wang M">MN Wang</name></author><author><name sortKey="Daszak, P" uniqKey="Daszak P">P Daszak</name></author><author><name sortKey="Wang, Lf" uniqKey="Wang L">LF Wang</name></author><author><name sortKey="Cui, J" uniqKey="Cui J">J Cui</name></author><author><name sortKey="Shi, Zl" uniqKey="Shi Z">ZL Shi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="De Souza Luna, Lk" uniqKey="De Souza Luna L">LK de Souza Luna</name></author><author><name sortKey="Heiser, V" uniqKey="Heiser V">V Heiser</name></author><author><name sortKey="Regamey, N" uniqKey="Regamey N">N Regamey</name></author><author><name sortKey="Panning, M" uniqKey="Panning M">M Panning</name></author><author><name sortKey="Drexler, Jf" uniqKey="Drexler J">JF Drexler</name></author><author><name sortKey="Mulangu, S" uniqKey="Mulangu S">S Mulangu</name></author><author><name sortKey="Poon, L" uniqKey="Poon L">L Poon</name></author><author><name sortKey="Baumgarte, S" uniqKey="Baumgarte S">S Baumgarte</name></author><author><name sortKey="Haijema, Bj" uniqKey="Haijema B">BJ Haijema</name></author><author><name sortKey="Kaiser, L" uniqKey="Kaiser L">L Kaiser</name></author><author><name sortKey="Drosten, C" uniqKey="Drosten C">C Drosten</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Drosten, C" uniqKey="Drosten C">C Drosten</name></author><author><name sortKey="Gunther, S" uniqKey="Gunther S">S Gunther</name></author><author><name sortKey="Preiser, W" uniqKey="Preiser W">W Preiser</name></author><author><name sortKey="Van Der Werf, S" uniqKey="Van Der Werf S">S van der Werf</name></author><author><name sortKey="Brodt, Hr" uniqKey="Brodt H">HR Brodt</name></author><author><name sortKey="Becker, S" uniqKey="Becker S">S Becker</name></author><author><name sortKey="Rabenau, H" uniqKey="Rabenau H">H Rabenau</name></author><author><name sortKey="Panning, M" uniqKey="Panning M">M Panning</name></author><author><name sortKey="Kolesnikova, L" uniqKey="Kolesnikova L">L Kolesnikova</name></author><author><name sortKey="Fouchier, Ram" uniqKey="Fouchier R">RAM Fouchier</name></author><author><name sortKey="Berger, A" uniqKey="Berger A">A Berger</name></author><author><name sortKey="Burguiere, Am" uniqKey="Burguiere A">AM Burguiere</name></author><author><name sortKey="Cinatl, J" uniqKey="Cinatl J">J Cinatl</name></author><author><name sortKey="Eickmann, M" uniqKey="Eickmann M">M Eickmann</name></author><author><name sortKey="Escriou, N" uniqKey="Escriou N">N Escriou</name></author><author><name sortKey="Grywna, K" uniqKey="Grywna K">K Grywna</name></author><author><name sortKey="Kramme, S" uniqKey="Kramme S">S Kramme</name></author><author><name sortKey="Manuguerra, Jc" uniqKey="Manuguerra J">JC Manuguerra</name></author><author><name sortKey="Muller, S" uniqKey="Muller S">S Muller</name></author><author><name sortKey="Rickerts, V" uniqKey="Rickerts V">V Rickerts</name></author><author><name sortKey="Sturmer, M" uniqKey="Sturmer M">M Sturmer</name></author><author><name sortKey="Vieth, S" uniqKey="Vieth S">S Vieth</name></author><author><name sortKey="Klenk, Hd" uniqKey="Klenk H">HD Klenk</name></author><author><name sortKey="Osterhaus, A" uniqKey="Osterhaus A">A Osterhaus</name></author><author><name sortKey="Schmitz, H" uniqKey="Schmitz H">H Schmitz</name></author><author><name sortKey="Doerr, Hw" uniqKey="Doerr H">HW Doerr</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zaki, Am" uniqKey="Zaki A">AM Zaki</name></author><author><name sortKey="Van Boheemen, S" uniqKey="Van Boheemen S">S van Boheemen</name></author><author><name sortKey="Bestebroer, Tm" uniqKey="Bestebroer T">TM Bestebroer</name></author><author><name sortKey="Osterhaus, A" uniqKey="Osterhaus A">A Osterhaus</name></author><author><name sortKey="Fouchier, R" uniqKey="Fouchier R">R Fouchier</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhou, P" uniqKey="Zhou P">P Zhou</name></author><author><name sortKey="Fan, H" uniqKey="Fan H">H Fan</name></author><author><name sortKey="Lan, T" uniqKey="Lan T">T Lan</name></author><author><name sortKey="Yang, Xl" uniqKey="Yang X">XL Yang</name></author><author><name sortKey="Shi, Wf" uniqKey="Shi W">WF Shi</name></author><author><name sortKey="Zhang, W" uniqKey="Zhang W">W Zhang</name></author><author><name sortKey="Zhu, Y" uniqKey="Zhu Y">Y Zhu</name></author><author><name sortKey="Zhang, Yw" uniqKey="Zhang Y">YW Zhang</name></author><author><name sortKey="Xie, Qm" uniqKey="Xie Q">QM Xie</name></author><author><name sortKey="Mani, S" uniqKey="Mani S">S Mani</name></author><author><name sortKey="Zheng, Xs" uniqKey="Zheng X">XS Zheng</name></author><author><name sortKey="Li, B" uniqKey="Li B">B Li</name></author><author><name sortKey="Li, Jm" uniqKey="Li J">JM Li</name></author><author><name sortKey="Guo, H" uniqKey="Guo H">H Guo</name></author><author><name sortKey="Pei, Gq" uniqKey="Pei G">GQ Pei</name></author><author><name sortKey="An, Xp" uniqKey="An X">XP An</name></author><author><name sortKey="Chen, Jw" uniqKey="Chen J">JW Chen</name></author><author><name sortKey="Zhou, L" uniqKey="Zhou L">L Zhou</name></author><author><name sortKey="Mai, Kj" uniqKey="Mai K">KJ Mai</name></author><author><name sortKey="Wu, Zx" uniqKey="Wu Z">ZX Wu</name></author><author><name sortKey="Li, D" uniqKey="Li D">D Li</name></author><author><name sortKey="Anderson, De" uniqKey="Anderson D">DE Anderson</name></author><author><name sortKey="Zhang, Lb" uniqKey="Zhang L">LB Zhang</name></author><author><name sortKey="Li, Sy" uniqKey="Li S">SY Li</name></author><author><name sortKey="Mi, Zq" uniqKey="Mi Z">ZQ Mi</name></author><author><name sortKey="He, Tt" uniqKey="He T">TT He</name></author><author><name sortKey="Cong, F" uniqKey="Cong F">F Cong</name></author><author><name sortKey="Guo, Pj" uniqKey="Guo P">PJ Guo</name></author><author><name sortKey="Huang, R" uniqKey="Huang R">R Huang</name></author><author><name sortKey="Luo, Y" uniqKey="Luo Y">Y Luo</name></author><author><name sortKey="Liu, Xl" uniqKey="Liu X">XL Liu</name></author><author><name sortKey="Chen, J" uniqKey="Chen J">J Chen</name></author><author><name sortKey="Huang, Y" uniqKey="Huang Y">Y Huang</name></author><author><name sortKey="Sun, Q" uniqKey="Sun Q">Q Sun</name></author><author><name sortKey="Zhang, Xll" uniqKey="Zhang X">XLL Zhang</name></author><author><name sortKey="Wang, Yy" uniqKey="Wang Y">YY Wang</name></author><author><name sortKey="Xing, Sz" uniqKey="Xing S">SZ Xing</name></author><author><name sortKey="Chen, Ys" uniqKey="Chen Y">YS Chen</name></author><author><name sortKey="Sun, Y" uniqKey="Sun Y">Y Sun</name></author><author><name sortKey="Li, J" uniqKey="Li J">J Li</name></author><author><name sortKey="Daszak, P" uniqKey="Daszak P">P Daszak</name></author><author><name sortKey="Wang, Lf" uniqKey="Wang L">LF Wang</name></author><author><name sortKey="Shi, Zl" uniqKey="Shi Z">ZL Shi</name></author><author><name sortKey="Tong, Yg" uniqKey="Tong Y">YG Tong</name></author><author><name sortKey="Ma, Jy" uniqKey="Ma J">JY Ma</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, L F" uniqKey="Wang L">L-F Wang</name></author><author><name sortKey="Cowled, C" uniqKey="Cowled C">C Cowled</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fan, Y" uniqKey="Fan Y">Y Fan</name></author><author><name sortKey="Zhao, K" uniqKey="Zhao K">K Zhao</name></author><author><name sortKey="Shi, Zl" uniqKey="Shi Z">ZL Shi</name></author><author><name sortKey="Zhou, P" uniqKey="Zhou P">P Zhou</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Carroll, D" uniqKey="Carroll D">D Carroll</name></author><author><name sortKey="Daszak, P" uniqKey="Daszak P">P Daszak</name></author><author><name sortKey="Wolfe, Nd" uniqKey="Wolfe N">ND Wolfe</name></author><author><name sortKey="Gao, Gf" uniqKey="Gao G">GF Gao</name></author><author><name sortKey="Morel, Cm" uniqKey="Morel C">CM Morel</name></author><author><name sortKey="Morzaria, S" uniqKey="Morzaria S">S Morzaria</name></author><author><name sortKey="Pablos Mendez, A" uniqKey="Pablos Mendez A">A Pablos-Mendez</name></author><author><name sortKey="Tomori, O" uniqKey="Tomori O">O Tomori</name></author><author><name sortKey="Mazet, J" uniqKey="Mazet J">J Mazet</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Briese, T" uniqKey="Briese T">T Briese</name></author><author><name sortKey="Kapoor, A" uniqKey="Kapoor A">A Kapoor</name></author><author><name sortKey="Mishra, N" uniqKey="Mishra N">N Mishra</name></author><author><name sortKey="Jain, K" uniqKey="Jain K">K Jain</name></author><author><name sortKey="Kumar, A" uniqKey="Kumar A">A Kumar</name></author><author><name sortKey="Jabado, Oj" uniqKey="Jabado O">OJ Jabado</name></author><author><name sortKey="Lipkin, Wi" uniqKey="Lipkin W">WI Lipkin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gaudin, M" uniqKey="Gaudin M">M Gaudin</name></author><author><name sortKey="Desnues, C" uniqKey="Desnues C">C Desnues</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wylie, Tn" uniqKey="Wylie T">TN Wylie</name></author><author><name sortKey="Wylie, Km" uniqKey="Wylie K">KM Wylie</name></author><author><name sortKey="Herter, Bn" uniqKey="Herter B">BN Herter</name></author><author><name sortKey="Storch, Ga" uniqKey="Storch G">GA Storch</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lim, Xf" uniqKey="Lim X">XF Lim</name></author><author><name sortKey="Lee, Cb" uniqKey="Lee C">CB Lee</name></author><author><name sortKey="Pascoe, Sm" uniqKey="Pascoe S">SM Pascoe</name></author><author><name sortKey="How, Cb" uniqKey="How C">CB How</name></author><author><name sortKey="Chan, S" uniqKey="Chan S">S Chan</name></author><author><name sortKey="Tan, Jh" uniqKey="Tan J">JH Tan</name></author><author><name sortKey="Yang, X" uniqKey="Yang X">X Yang</name></author><author><name sortKey="Zhou, P" uniqKey="Zhou P">P Zhou</name></author><author><name sortKey="Shi, Z" uniqKey="Shi Z">Z Shi</name></author><author><name sortKey="Sessions, Om" uniqKey="Sessions O">OM Sessions</name></author><author><name sortKey="Wang, Lf" uniqKey="Wang L">LF Wang</name></author><author><name sortKey="Ng, Lc" uniqKey="Ng L">LC Ng</name></author><author><name sortKey="Anderson, De" uniqKey="Anderson D">DE Anderson</name></author><author><name sortKey="Yap, G" uniqKey="Yap G">G Yap</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Noyes, Nr" uniqKey="Noyes N">NR Noyes</name></author><author><name sortKey="Weinroth, Me" uniqKey="Weinroth M">ME Weinroth</name></author><author><name sortKey="Parker, Jk" uniqKey="Parker J">JK Parker</name></author><author><name sortKey="Dean, Cj" uniqKey="Dean C">CJ Dean</name></author><author><name sortKey="Lakin, Sm" uniqKey="Lakin S">SM Lakin</name></author><author><name sortKey="Raymond, Ra" uniqKey="Raymond R">RA Raymond</name></author><author><name sortKey="Rovira, P" uniqKey="Rovira P">P Rovira</name></author><author><name sortKey="Doster, E" uniqKey="Doster E">E Doster</name></author><author><name sortKey="Abdo, Z" uniqKey="Abdo Z">Z Abdo</name></author><author><name sortKey="Martin, Jn" uniqKey="Martin J">JN Martin</name></author><author><name sortKey="Jones, Kl" uniqKey="Jones K">KL Jones</name></author><author><name sortKey="Ruiz, J" uniqKey="Ruiz J">J Ruiz</name></author><author><name sortKey="Boucher, Ca" uniqKey="Boucher C">CA Boucher</name></author><author><name sortKey="Belk, Ke" uniqKey="Belk K">KE Belk</name></author><author><name sortKey="Morley, Ps" uniqKey="Morley P">PS Morley</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Metsky, Hc" uniqKey="Metsky H">HC Metsky</name></author><author><name sortKey="Siddle, Kj" uniqKey="Siddle K">KJ Siddle</name></author><author><name sortKey="Gladden Young, A" uniqKey="Gladden Young A">A Gladden-Young</name></author><author><name sortKey="Qu, J" uniqKey="Qu J">J Qu</name></author><author><name sortKey="Yang, Dk" uniqKey="Yang D">DK Yang</name></author><author><name sortKey="Brehio, P" uniqKey="Brehio P">P Brehio</name></author><author><name sortKey="Goldfarb, A" uniqKey="Goldfarb A">A Goldfarb</name></author><author><name sortKey="Piantadosi, A" uniqKey="Piantadosi A">A Piantadosi</name></author><author><name sortKey="Wohl, S" uniqKey="Wohl S">S Wohl</name></author><author><name sortKey="Carter, A" uniqKey="Carter A">A Carter</name></author><author><name sortKey="Lin, Ae" uniqKey="Lin A">AE Lin</name></author><author><name sortKey="Barnes, Kg" uniqKey="Barnes K">KG Barnes</name></author><author><name sortKey="Tully, Dc" uniqKey="Tully D">DC Tully</name></author><author><name sortKey="Corleis, B" uniqKey="Corleis B">B Corleis</name></author><author><name sortKey="Hennigan, S" uniqKey="Hennigan S">S Hennigan</name></author><author><name sortKey="Barbosa Lima, G" uniqKey="Barbosa Lima G">G Barbosa-Lima</name></author><author><name sortKey="Vieira, Yr" uniqKey="Vieira Y">YR Vieira</name></author><author><name sortKey="Paul, Lm" uniqKey="Paul L">LM Paul</name></author><author><name sortKey="Tan, Al" uniqKey="Tan A">AL Tan</name></author><author><name sortKey="Garcia, Kf" uniqKey="Garcia K">KF Garcia</name></author><author><name sortKey="Parham, La" uniqKey="Parham L">LA Parham</name></author><author><name sortKey="Odia, I" uniqKey="Odia I">I Odia</name></author><author><name sortKey="Eromon, P" uniqKey="Eromon P">P Eromon</name></author><author><name sortKey="Folarin, Oa" uniqKey="Folarin O">OA Folarin</name></author><author><name sortKey="Goba, A" uniqKey="Goba A">A Goba</name></author><author><name sortKey="Simon Loriere, E" uniqKey="Simon Loriere E">E Simon-Loriere</name></author><author><name sortKey="Hensley, L" uniqKey="Hensley L">L Hensley</name></author><author><name sortKey="Balmaseda, A" uniqKey="Balmaseda A">A Balmaseda</name></author><author><name sortKey="Harris, E" uniqKey="Harris E">E Harris</name></author><author><name sortKey="Kwon, Ds" uniqKey="Kwon D">DS Kwon</name></author><author><name sortKey="Allen, Tm" uniqKey="Allen T">TM Allen</name></author><author><name sortKey="Runstadler, Ja" uniqKey="Runstadler J">JA Runstadler</name></author><author><name sortKey="Smole, S" uniqKey="Smole S">S Smole</name></author><author><name sortKey="Bozza, Fa" uniqKey="Bozza F">FA Bozza</name></author><author><name sortKey="Souza, Tml" uniqKey="Souza T">TML Souza</name></author><author><name sortKey="Isern, S" uniqKey="Isern S">S Isern</name></author><author><name sortKey="Michael, Sf" uniqKey="Michael S">SF Michael</name></author><author><name sortKey="Lorenzana, I" uniqKey="Lorenzana I">I Lorenzana</name></author><author><name sortKey="Gehrke, L" uniqKey="Gehrke L">L Gehrke</name></author><author><name sortKey="Bosch, I" uniqKey="Bosch I">I Bosch</name></author><author><name sortKey="Ebel, G" uniqKey="Ebel G">G Ebel</name></author><author><name sortKey="Grant, Ds" uniqKey="Grant D">DS Grant</name></author><author><name sortKey="Happi, Ct" uniqKey="Happi C">CT Happi</name></author><author><name sortKey="Park, Dj" uniqKey="Park D">DJ Park</name></author><author><name sortKey="Gnirke, A" uniqKey="Gnirke A">A Gnirke</name></author><author><name sortKey="Sabeti, Pc" uniqKey="Sabeti P">PC Sabeti</name></author><author><name sortKey="Matranga, Cb" uniqKey="Matranga C">CB Matranga</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lau, Sk" uniqKey="Lau S">SK Lau</name></author><author><name sortKey="Woo, Pc" uniqKey="Woo P">PC Woo</name></author><author><name sortKey="Li, Ks" uniqKey="Li K">KS Li</name></author><author><name sortKey="Huang, Y" uniqKey="Huang Y">Y Huang</name></author><author><name sortKey="Wang, M" uniqKey="Wang M">M Wang</name></author><author><name sortKey="Lam, Cs" uniqKey="Lam C">CS Lam</name></author><author><name sortKey="Xu, H" uniqKey="Xu H">H Xu</name></author><author><name sortKey="Guo, R" uniqKey="Guo R">R Guo</name></author><author><name sortKey="Chan, Kh" uniqKey="Chan K">KH Chan</name></author><author><name sortKey="Zheng, Bj" uniqKey="Zheng B">BJ Zheng</name></author><author><name sortKey="Yuen, Ky" uniqKey="Yuen K">KY Yuen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, W" uniqKey="Zhang W">W Zhang</name></author><author><name sortKey="Zheng, Xs" uniqKey="Zheng X">XS Zheng</name></author><author><name sortKey="Agwanda, B" uniqKey="Agwanda B">B Agwanda</name></author><author><name sortKey="Ommeh, S" uniqKey="Ommeh S">S Ommeh</name></author><author><name sortKey="Zhao, K" uniqKey="Zhao K">K Zhao</name></author><author><name sortKey="Lichoti, J" uniqKey="Lichoti J">J Lichoti</name></author><author><name sortKey="Wang, N" uniqKey="Wang N">N Wang</name></author><author><name sortKey="Chen, J" uniqKey="Chen J">J Chen</name></author><author><name sortKey="Li, B" uniqKey="Li B">B Li</name></author><author><name sortKey="Yang, Xl" uniqKey="Yang X">XL Yang</name></author><author><name sortKey="Mani, S" uniqKey="Mani S">S Mani</name></author><author><name sortKey="Ngeiywa, Kj" uniqKey="Ngeiywa K">KJ Ngeiywa</name></author><author><name sortKey="Zhu, Y" uniqKey="Zhu Y">Y Zhu</name></author><author><name sortKey="Hu, B" uniqKey="Hu B">B Hu</name></author><author><name sortKey="Onyuok, So" uniqKey="Onyuok S">SO Onyuok</name></author><author><name sortKey="Yan, B" uniqKey="Yan B">B Yan</name></author><author><name sortKey="Anderson, De" uniqKey="Anderson D">DE Anderson</name></author><author><name sortKey="Wang, Lf" uniqKey="Wang L">LF Wang</name></author><author><name sortKey="Zhou, P" uniqKey="Zhou P">P Zhou</name></author><author><name sortKey="Shi, Zl" uniqKey="Shi Z">ZL Shi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Luo, C M" uniqKey="Luo C">C-M Luo</name></author><author><name sortKey="Wang, N" uniqKey="Wang N">N Wang</name></author><author><name sortKey="Yang, X L" uniqKey="Yang X">X-L Yang</name></author><author><name sortKey="Liu, H Z" uniqKey="Liu H">H-Z Liu</name></author><author><name sortKey="Zhang, W" uniqKey="Zhang W">W Zhang</name></author><author><name sortKey="Li, B" uniqKey="Li B">B Li</name></author><author><name sortKey="Hu, B" uniqKey="Hu B">B Hu</name></author><author><name sortKey="Peng, C" uniqKey="Peng C">C Peng</name></author><author><name sortKey="Geng, Q B" uniqKey="Geng Q">Q-B Geng</name></author><author><name sortKey="Zhu, G J" uniqKey="Zhu G">G-J Zhu</name></author><author><name sortKey="Li, F" uniqKey="Li F">F Li</name></author><author><name sortKey="Shi, Z L" uniqKey="Shi Z">Z-L Shi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ge, Xy" uniqKey="Ge X">XY Ge</name></author><author><name sortKey="Wang, N" uniqKey="Wang N">N Wang</name></author><author><name sortKey="Zhang, W" uniqKey="Zhang W">W Zhang</name></author><author><name sortKey="Hu, B" uniqKey="Hu B">B Hu</name></author><author><name sortKey="Li, B" uniqKey="Li B">B Li</name></author><author><name sortKey="Zhang, Yz" uniqKey="Zhang Y">YZ Zhang</name></author><author><name sortKey="Zhou, Jh" uniqKey="Zhou J">JH Zhou</name></author><author><name sortKey="Luo, Cm" uniqKey="Luo C">CM Luo</name></author><author><name sortKey="Yang, Xl" uniqKey="Yang X">XL Yang</name></author><author><name sortKey="Wu, Lj" uniqKey="Wu L">LJ Wu</name></author><author><name sortKey="Wang, B" uniqKey="Wang B">B Wang</name></author><author><name sortKey="Zhang, Y" uniqKey="Zhang Y">Y Zhang</name></author><author><name sortKey="Li, Zx" uniqKey="Li Z">ZX Li</name></author><author><name sortKey="Shi, Zl" uniqKey="Shi Z">ZL Shi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Goecks, J" uniqKey="Goecks J">J Goecks</name></author><author><name sortKey="Nekrutenko, A" uniqKey="Nekrutenko A">A Nekrutenko</name></author><author><name sortKey="Taylor, J" uniqKey="Taylor J">J Taylor</name></author></analytic></biblStruct></listBibl></div1></back></TEI></pmc><pubmed><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Discovery of Bat Coronaviruses through Surveillance and Probe Capture-Based Next-Generation Sequencing.</title><author><name sortKey="Li, Bei" sort="Li, Bei" uniqKey="Li B" first="Bei" last="Li">Bei Li</name><affiliation wicri:level="3"><nlm:affiliation>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Si, Hao Rui" sort="Si, Hao Rui" uniqKey="Si H" first="Hao-Rui" last="Si">Hao-Rui Si</name><affiliation wicri:level="3"><nlm:affiliation>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Zhu, Yan" sort="Zhu, Yan" uniqKey="Zhu Y" first="Yan" last="Zhu">Yan Zhu</name><affiliation wicri:level="3"><nlm:affiliation>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Yang, Xing Lou" sort="Yang, Xing Lou" uniqKey="Yang X" first="Xing-Lou" last="Yang">Xing-Lou Yang</name><affiliation wicri:level="3"><nlm:affiliation>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Anderson, Danielle E" sort="Anderson, Danielle E" uniqKey="Anderson D" first="Danielle E" last="Anderson">Danielle E. Anderson</name><affiliation wicri:level="1"><nlm:affiliation>Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore, Singapore.</nlm:affiliation><country xml:lang="fr">Singapour</country><wicri:regionArea>Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore</wicri:regionArea><wicri:noRegion>Singapore</wicri:noRegion></affiliation></author><author><name sortKey="Shi, Zheng Li" sort="Shi, Zheng Li" uniqKey="Shi Z" first="Zheng-Li" last="Shi">Zheng-Li Shi</name><affiliation wicri:level="3"><nlm:affiliation>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Wang, Lin Fa" sort="Wang, Lin Fa" uniqKey="Wang L" first="Lin-Fa" last="Wang">Lin-Fa Wang</name><affiliation wicri:level="1"><nlm:affiliation>Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore, Singapore linfa.wang@duke-nus.edu.sg peng.zhou@wh.iov.cn.</nlm:affiliation><country wicri:rule="url">Singapour</country><wicri:regionArea>Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore</wicri:regionArea><wicri:noRegion>Singapore</wicri:noRegion></affiliation></author><author><name sortKey="Zhou, Peng" sort="Zhou, Peng" uniqKey="Zhou P" first="Peng" last="Zhou">Peng Zhou</name><affiliation wicri:level="3"><nlm:affiliation>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China linfa.wang@duke-nus.edu.sg peng.zhou@wh.iov.cn.</nlm:affiliation><country wicri:rule="url">Singapour</country><wicri:regionArea>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:31996413</idno><idno type="pmid">31996413</idno><idno type="doi">10.1128/mSphere.00807-19</idno><idno type="wicri:Area/PubMed/Corpus">000833</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000833</idno><idno type="wicri:Area/PubMed/Curation">000833</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000833</idno><idno type="wicri:Area/PubMed/Checkpoint">000564</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000564</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Discovery of Bat Coronaviruses through Surveillance and Probe Capture-Based Next-Generation Sequencing.</title><author><name sortKey="Li, Bei" sort="Li, Bei" uniqKey="Li B" first="Bei" last="Li">Bei Li</name><affiliation wicri:level="3"><nlm:affiliation>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Si, Hao Rui" sort="Si, Hao Rui" uniqKey="Si H" first="Hao-Rui" last="Si">Hao-Rui Si</name><affiliation wicri:level="3"><nlm:affiliation>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Zhu, Yan" sort="Zhu, Yan" uniqKey="Zhu Y" first="Yan" last="Zhu">Yan Zhu</name><affiliation wicri:level="3"><nlm:affiliation>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Yang, Xing Lou" sort="Yang, Xing Lou" uniqKey="Yang X" first="Xing-Lou" last="Yang">Xing-Lou Yang</name><affiliation wicri:level="3"><nlm:affiliation>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Anderson, Danielle E" sort="Anderson, Danielle E" uniqKey="Anderson D" first="Danielle E" last="Anderson">Danielle E. Anderson</name><affiliation wicri:level="1"><nlm:affiliation>Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore, Singapore.</nlm:affiliation><country xml:lang="fr">Singapour</country><wicri:regionArea>Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore</wicri:regionArea><wicri:noRegion>Singapore</wicri:noRegion></affiliation></author><author><name sortKey="Shi, Zheng Li" sort="Shi, Zheng Li" uniqKey="Shi Z" first="Zheng-Li" last="Shi">Zheng-Li Shi</name><affiliation wicri:level="3"><nlm:affiliation>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Wang, Lin Fa" sort="Wang, Lin Fa" uniqKey="Wang L" first="Lin-Fa" last="Wang">Lin-Fa Wang</name><affiliation wicri:level="1"><nlm:affiliation>Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore, Singapore linfa.wang@duke-nus.edu.sg peng.zhou@wh.iov.cn.</nlm:affiliation><country wicri:rule="url">Singapour</country><wicri:regionArea>Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore</wicri:regionArea><wicri:noRegion>Singapore</wicri:noRegion></affiliation></author><author><name sortKey="Zhou, Peng" sort="Zhou, Peng" uniqKey="Zhou P" first="Peng" last="Zhou">Peng Zhou</name><affiliation wicri:level="3"><nlm:affiliation>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China linfa.wang@duke-nus.edu.sg peng.zhou@wh.iov.cn.</nlm:affiliation><country wicri:rule="url">Singapour</country><wicri:regionArea>CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author></analytic><series><title level="j">mSphere</title><idno type="eISSN">2379-5042</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Chiroptera (virology)</term><term>Coronavirus (classification)</term><term>Coronavirus (isolation & purification)</term><term>Genetic Variation</term><term>Genome, Viral</term><term>High-Throughput Nucleotide Sequencing (methods)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Chiroptera (virologie)</term><term>Coronavirus ()</term><term>Coronavirus (isolement et purification)</term><term>Génome viral</term><term>Séquençage nucléotidique à haut débit ()</term><term>Variation génétique</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>High-Throughput Nucleotide Sequencing</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Chiroptera</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Chiroptera</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Genetic Variation</term><term>Genome, Viral</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Coronavirus</term><term>Génome viral</term><term>Séquençage nucléotidique à haut débit</term><term>Variation génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Coronaviruses (CoVs) of bat origin have caused two pandemics in this century. Severe acute respiratory syndrome (SARS)-CoV and Middle East respiratory syndrome (MERS)-CoV both originated from bats, and it is highly likely that bat coronaviruses will cause future outbreaks. Active surveillance is both urgent and essential to predict and mitigate the emergence of these viruses in humans. Next-generation sequencing (NGS) is currently the preferred methodology for virus discovery to ensure unbiased sequencing of bat CoVs, considering their high genetic diversity. However, unbiased NGS is an expensive methodology and is prone to missing low-abundance CoV sequences due to the high background level of nonviral sequences present in surveillance field samples. Here, we employ a capture-based NGS approach using baits targeting most of the CoV species. Using this technology, we effectively reduced sequencing costs by increasing the sensitivity of detection. We discovered nine full genomes of bat CoVs in this study and revealed great genetic diversity for eight of them.<b>IMPORTANCE</b> Active surveillance is both urgent and essential to predict and mitigate the emergence of bat-origin CoV in humans and livestock. However, great genetic diversity increases the chance of homologous recombination among CoVs. Performing targeted PCR, a common practice for many surveillance studies, would not reflect this diversity. NGS, on the other hand, is an expensive methodology and is prone to missing low-abundance CoV sequences. Here, we employ a capture-based NGS approach using baits targeting all CoVs. Our work demonstrates that targeted, cost-effective, large-scale, genome-level surveillance of bat CoVs is now highly feasible.</div></front></TEI></pubmed></double></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Ncbi/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003134 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd -nk 003134 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Ncbi
|étape= Merge
|type= RBID
|clé= PMC:6992374
|texte= Discovery of Bat Coronaviruses through Surveillance and Probe Capture-Based Next-Generation Sequencing
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/RBID.i -Sk "pubmed:31996413" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |