From Cells to Virus Particles: Quantitative Methods to Monitor RNA Packaging.
Identifieur interne : 002D00 ( Ncbi/Merge ); précédent : 002C99; suivant : 002D01From Cells to Virus Particles: Quantitative Methods to Monitor RNA Packaging.
Auteurs : Mireia Ferrer [France] ; Simon Henriet [Norvège] ; Célia Chamontin [France] ; Sébastien Lainé [France] ; Marylène Mougel [France]Source :
- Viruses [ 1999-4915 ] ; 2016.
Descripteurs français
- KwdFr :
- MESH :
- métabolisme : ARN viral, Virion.
- physiologie : Retroviridae.
- Animaux, Assemblage viral, Biologie moléculaire, Humains, Virologie.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : RNA, Viral.
- metabolism : Virion.
- methods : Molecular Biology, Virology.
- physiology : Retroviridae.
- Animals, Humans, Virus Assembly.
Abstract
In cells, positive strand RNA viruses, such as Retroviridae, must selectively recognize their full-length RNA genome among abundant cellular RNAs to assemble and release particles. How viruses coordinate the intracellular trafficking of both RNA and protein components to the assembly sites of infectious particles at the cell surface remains a long-standing question. The mechanisms ensuring packaging of genomic RNA are essential for viral infectivity. Since RNA packaging impacts on several essential functions of retroviral replication such as RNA dimerization, translation and recombination events, there are many studies that require the determination of RNA packaging efficiency and/or RNA packaging ability. Studies of RNA encapsidation rely upon techniques for the identification and quantification of RNA species packaged by the virus. This review focuses on the different approaches available to monitor RNA packaging: Northern blot analysis, ribonuclease protection assay and quantitative reverse transcriptase-coupled polymerase chain reaction as well as the most recent RNA imaging and sequencing technologies. Advantages, disadvantages and limitations of these approaches will be discussed in order to help the investigator to choose the most appropriate technique. Although the review was written with the prototypic simple murine leukemia virus (MLV) and complex human immunodeficiency virus type 1 (HIV-1) in mind, the techniques were described in order to benefit to a larger community.
DOI: 10.3390/v8080239
PubMed: 27556480
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Simon.Henriet@uib.no.</nlm:affiliation><country xml:lang="fr">Norvège</country><wicri:regionArea>Sars International Centre for Marine Molecular Biology, University of Bergen, Bergen 5018</wicri:regionArea><wicri:noRegion>Bergen 5018</wicri:noRegion></affiliation></author><author><name sortKey="Chamontin, Celia" sort="Chamontin, Celia" uniqKey="Chamontin C" first="Célia" last="Chamontin">Célia Chamontin</name><affiliation wicri:level="3"><nlm:affiliation>CPBS, CNRS, Université de Montpellier, 1919 Route de Mende, Montpellier 34293, France. celia.chamontin@cpbs.cnrs.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>CPBS, CNRS, Université de Montpellier, 1919 Route de Mende, Montpellier 34293</wicri:regionArea><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Languedoc-Roussillon</region></placeName></affiliation></author><author><name sortKey="Laine, Sebastien" sort="Laine, Sebastien" uniqKey="Laine S" first="Sébastien" last="Lainé">Sébastien Lainé</name><affiliation wicri:level="3"><nlm:affiliation>CPBS, CNRS, Université de Montpellier, 1919 Route de Mende, Montpellier 34293, France. sebastien.laine@cpbs.cnrs.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>CPBS, CNRS, Université de Montpellier, 1919 Route de Mende, Montpellier 34293</wicri:regionArea><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Languedoc-Roussillon</region></placeName></affiliation></author><author><name sortKey="Mougel, Marylene" sort="Mougel, Marylene" uniqKey="Mougel M" first="Marylène" last="Mougel">Marylène Mougel</name><affiliation wicri:level="3"><nlm:affiliation>CPBS, CNRS, Université de Montpellier, 1919 Route de Mende, Montpellier 34293, France. marylene.mougel@cpbs.cnrs.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>CPBS, CNRS, Université de Montpellier, 1919 Route de Mende, Montpellier 34293</wicri:regionArea><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Languedoc-Roussillon</region></placeName></affiliation></author></analytic><series><title level="j">Viruses</title><idno type="eISSN">1999-4915</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Humans</term><term>Molecular Biology (methods)</term><term>RNA, Viral (metabolism)</term><term>Retroviridae (physiology)</term><term>Virion (metabolism)</term><term>Virology (methods)</term><term>Virus Assembly</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (métabolisme)</term><term>Animaux</term><term>Assemblage viral</term><term>Biologie moléculaire ()</term><term>Humains</term><term>Retroviridae (physiologie)</term><term>Virion (métabolisme)</term><term>Virologie ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Virion</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Molecular Biology</term><term>Virology</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN viral</term><term>Virion</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Retroviridae</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Retroviridae</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Humans</term><term>Virus Assembly</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Assemblage viral</term><term>Biologie moléculaire</term><term>Humains</term><term>Virologie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In cells, positive strand RNA viruses, such as Retroviridae, must selectively recognize their full-length RNA genome among abundant cellular RNAs to assemble and release particles. How viruses coordinate the intracellular trafficking of both RNA and protein components to the assembly sites of infectious particles at the cell surface remains a long-standing question. The mechanisms ensuring packaging of genomic RNA are essential for viral infectivity. Since RNA packaging impacts on several essential functions of retroviral replication such as RNA dimerization, translation and recombination events, there are many studies that require the determination of RNA packaging efficiency and/or RNA packaging ability. Studies of RNA encapsidation rely upon techniques for the identification and quantification of RNA species packaged by the virus. This review focuses on the different approaches available to monitor RNA packaging: Northern blot analysis, ribonuclease protection assay and quantitative reverse transcriptase-coupled polymerase chain reaction as well as the most recent RNA imaging and sequencing technologies. Advantages, disadvantages and limitations of these approaches will be discussed in order to help the investigator to choose the most appropriate technique. Although the review was written with the prototypic simple murine leukemia virus (MLV) and complex human immunodeficiency virus type 1 (HIV-1) in mind, the techniques were described in order to benefit to a larger community.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27556480</PMID><DateCompleted><Year>2017</Year><Month>09</Month><Day>26</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1999-4915</ISSN><JournalIssue CitedMedium="Internet"><Volume>8</Volume><Issue>8</Issue><PubDate><Year>2016</Year><Month>08</Month><Day>22</Day></PubDate></JournalIssue><Title>Viruses</Title><ISOAbbreviation>Viruses</ISOAbbreviation></Journal><ArticleTitle>From Cells to Virus Particles: Quantitative Methods to Monitor RNA Packaging.</ArticleTitle><ELocationID EIdType="doi" ValidYN="Y">10.3390/v8080239</ELocationID><ELocationID EIdType="pii" ValidYN="Y">E239</ELocationID><Abstract><AbstractText>In cells, positive strand RNA viruses, such as Retroviridae, must selectively recognize their full-length RNA genome among abundant cellular RNAs to assemble and release particles. How viruses coordinate the intracellular trafficking of both RNA and protein components to the assembly sites of infectious particles at the cell surface remains a long-standing question. The mechanisms ensuring packaging of genomic RNA are essential for viral infectivity. Since RNA packaging impacts on several essential functions of retroviral replication such as RNA dimerization, translation and recombination events, there are many studies that require the determination of RNA packaging efficiency and/or RNA packaging ability. Studies of RNA encapsidation rely upon techniques for the identification and quantification of RNA species packaged by the virus. This review focuses on the different approaches available to monitor RNA packaging: Northern blot analysis, ribonuclease protection assay and quantitative reverse transcriptase-coupled polymerase chain reaction as well as the most recent RNA imaging and sequencing technologies. Advantages, disadvantages and limitations of these approaches will be discussed in order to help the investigator to choose the most appropriate technique. Although the review was written with the prototypic simple murine leukemia virus (MLV) and complex human immunodeficiency virus type 1 (HIV-1) in mind, the techniques were described in order to benefit to a larger community.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ferrer</LastName><ForeName>Mireia</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>CPBS, CNRS, Université de Montpellier, 1919 Route de Mende, Montpellier 34293, France. mireia.dream@gmail.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Henriet</LastName><ForeName>Simon</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Sars International Centre for Marine Molecular Biology, University of Bergen, Bergen 5018, Norway. Simon.Henriet@uib.no.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chamontin</LastName><ForeName>Célia</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>CPBS, CNRS, Université de Montpellier, 1919 Route de Mende, Montpellier 34293, France. celia.chamontin@cpbs.cnrs.fr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lainé</LastName><ForeName>Sébastien</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>CPBS, CNRS, Université de Montpellier, 1919 Route de Mende, Montpellier 34293, France. sebastien.laine@cpbs.cnrs.fr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mougel</LastName><ForeName>Marylène</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>CPBS, CNRS, Université de Montpellier, 1919 Route de Mende, Montpellier 34293, France. marylene.mougel@cpbs.cnrs.fr.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D016454">Review</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>08</Month><Day>22</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Viruses</MedlineTA><NlmUniqueID>101509722</NlmUniqueID><ISSNLinking>1999-4915</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012367">RNA, Viral</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008967" MajorTopicYN="N">Molecular Biology</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012367" MajorTopicYN="N">RNA, Viral</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012190" MajorTopicYN="N">Retroviridae</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014771" MajorTopicYN="N">Virion</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014773" MajorTopicYN="N">Virology</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019065" MajorTopicYN="Y">Virus Assembly</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Gag</Keyword><Keyword MajorTopicYN="Y">HIV-1</Keyword><Keyword MajorTopicYN="Y">Northern blot</Keyword><Keyword MajorTopicYN="Y">RNA</Keyword><Keyword MajorTopicYN="Y">RNA imaging</Keyword><Keyword MajorTopicYN="Y">RT-qPCR</Keyword><Keyword MajorTopicYN="Y">assembly</Keyword><Keyword MajorTopicYN="Y">fluorescence microscopy</Keyword><Keyword MajorTopicYN="Y">packaging</Keyword><Keyword MajorTopicYN="Y">retrovirus</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year><Month>07</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2016</Year><Month>08</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>08</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>8</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>8</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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(région administrative)</li></region></list><tree><country name="France"><region name="Occitanie (région administrative)"><name sortKey="Ferrer, Mireia" sort="Ferrer, Mireia" uniqKey="Ferrer M" first="Mireia" last="Ferrer">Mireia Ferrer</name></region><name sortKey="Chamontin, Celia" sort="Chamontin, Celia" uniqKey="Chamontin C" first="Célia" last="Chamontin">Célia Chamontin</name><name sortKey="Laine, Sebastien" sort="Laine, Sebastien" uniqKey="Laine S" first="Sébastien" last="Lainé">Sébastien Lainé</name><name sortKey="Mougel, Marylene" sort="Mougel, Marylene" uniqKey="Mougel M" first="Marylène" last="Mougel">Marylène Mougel</name></country><country name="Norvège"><noRegion><name sortKey="Henriet, Simon" sort="Henriet, Simon" uniqKey="Henriet S" first="Simon" last="Henriet">Simon Henriet</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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