Identification of a Novel Small Molecule Inhibitor Against SARS Coronavirus Helicase.
Identifieur interne : 002B34 ( Ncbi/Merge ); précédent : 002B33; suivant : 002B35Identification of a Novel Small Molecule Inhibitor Against SARS Coronavirus Helicase.
Auteurs : Jin-Beom Cho [Corée du Sud] ; Jin-Moo Lee [Corée du Sud] ; Hee-Chul Ahn [Corée du Sud] ; Yong-Joo Jeong [Corée du Sud]Source :
- Journal of microbiology and biotechnology [ 1738-8872 ] ; 2015.
Descripteurs français
- KwdFr :
- MESH :
- antagonistes et inhibiteurs : Helicase.
- enzymologie : Virus du SRAS.
- isolement et purification : Antienzymes, Antiviraux.
- métabolisme : Adénosine triphosphate.
- pharmacologie : Antienzymes, Antiviraux.
- Concentration inhibitrice 50, Hydrolyse.
English descriptors
- KwdEn :
- Adenosine Triphosphate (metabolism), Antiviral Agents (isolation & purification), Antiviral Agents (pharmacology), DNA Helicases (antagonists & inhibitors), Enzyme Inhibitors (isolation & purification), Enzyme Inhibitors (pharmacology), Hydrolysis, Inhibitory Concentration 50, SARS Virus (enzymology).
- MESH :
- chemical , antagonists & inhibitors : DNA Helicases.
- chemical , isolation & purification : Antiviral Agents, Enzyme Inhibitors.
- chemical , metabolism : Adenosine Triphosphate.
- chemical , pharmacology : Antiviral Agents, Enzyme Inhibitors.
- enzymology : SARS Virus.
- Hydrolysis, Inhibitory Concentration 50.
Abstract
A new chemical inhibitor against severe acute respiratory syndrome (SARS) coronavirus helicase, 7-ethyl-8-mercapto-3-methyl-3,7-dihydro-1H-purine-2,6-dione, was identified. We investigated the inhibitory effect of the compound by conducting colorimetry-based ATP hydrolysis assay and fluorescence resonance energy transfer-based double-stranded DNA unwinding assay. The compound suppressed both ATP hydrolysis and double-stranded DNA unwinding activities of helicase with IC50 values of 8.66 ± 0.26 μM and 41.6 ± 2.3 μM, respectively. Moreover, we observed that the compound did not show cytotoxicity up to 80 µMconcentration. Our results suggest that the compound might serve as a SARS coronavirus inhibitor.
DOI: 10.4014/jmb.1507.07078
PubMed: 26387819
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Y" first="Yong-Joo" last="Jeong">Yong-Joo Jeong</name><affiliation wicri:level="1"><nlm:affiliation>Department of Bio and Nanochemistry, Kookmin University, Seoul 136-702, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Bio and Nanochemistry, Kookmin University, Seoul 136-702</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author></analytic><series><title level="j">Journal of microbiology and biotechnology</title><idno type="eISSN">1738-8872</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adenosine Triphosphate (metabolism)</term><term>Antiviral Agents (isolation & purification)</term><term>Antiviral Agents (pharmacology)</term><term>DNA Helicases (antagonists & 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50</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Concentration inhibitrice 50</term><term>Hydrolyse</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A new chemical inhibitor against severe acute respiratory syndrome (SARS) coronavirus helicase, 7-ethyl-8-mercapto-3-methyl-3,7-dihydro-1H-purine-2,6-dione, was identified. We investigated the inhibitory effect of the compound by conducting colorimetry-based ATP hydrolysis assay and fluorescence resonance energy transfer-based double-stranded DNA unwinding assay. The compound suppressed both ATP hydrolysis and double-stranded DNA unwinding activities of helicase with IC50 values of 8.66 ± 0.26 μM and 41.6 ± 2.3 μM, respectively. Moreover, we observed that the compound did not show cytotoxicity up to 80 µMconcentration. Our results suggest that the compound might serve as a SARS coronavirus inhibitor. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26387819</PMID><DateCompleted><Year>2016</Year><Month>10</Month><Day>06</Day></DateCompleted><DateRevised><Year>2016</Year><Month>12</Month><Day>30</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1738-8872</ISSN><JournalIssue CitedMedium="Internet"><Volume>25</Volume><Issue>12</Issue><PubDate><Year>2015</Year><Month>Dec</Month><Day>28</Day></PubDate></JournalIssue><Title>Journal of microbiology and biotechnology</Title><ISOAbbreviation>J. Microbiol. 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Our results suggest that the compound might serve as a SARS coronavirus inhibitor. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Cho</LastName><ForeName>Jin-Beom</ForeName><Initials>JB</Initials><AffiliationInfo><Affiliation>Department of Bio and Nanochemistry, Kookmin University, Seoul 136-702, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Jin-Moo</ForeName><Initials>JM</Initials><AffiliationInfo><Affiliation>Department of Bio and Nanochemistry, Kookmin University, Seoul 136-702, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ahn</LastName><ForeName>Hee-Chul</ForeName><Initials>HC</Initials><AffiliationInfo><Affiliation>Department of Pharmacy, Dongguk University-Seoul, Goyang 410-820, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author 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PubStatus="pubmed"><Year>2015</Year><Month>9</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>10</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26387819</ArticleId><ArticleId IdType="pii">10.4014/jmb.1507.07078</ArticleId><ArticleId IdType="doi">10.4014/jmb.1507.07078</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Corée du Sud</li></country><region><li>Région capitale de Séoul</li></region><settlement><li>Séoul</li></settlement></list><tree><country name="Corée du Sud"><region name="Région capitale de Séoul"><name sortKey="Cho, Jin Beom" sort="Cho, Jin Beom" uniqKey="Cho J" first="Jin-Beom" last="Cho">Jin-Beom Cho</name></region><name sortKey="Ahn, Hee Chul" sort="Ahn, Hee Chul" uniqKey="Ahn H" first="Hee-Chul" last="Ahn">Hee-Chul Ahn</name><name sortKey="Jeong, Yong Joo" sort="Jeong, Yong Joo" uniqKey="Jeong Y" first="Yong-Joo" last="Jeong">Yong-Joo Jeong</name><name sortKey="Lee, Jin Moo" sort="Lee, Jin Moo" uniqKey="Lee J" first="Jin-Moo" last="Lee">Jin-Moo Lee</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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