SrasV1, Ncbi, Merge, bibRecord, 002644

Tropism of and innate immune responses to the novel human betacoronavirus lineage C virus in human ex vivo respiratory organ cultures.

Identifieur interne : 002644 ( Ncbi/Merge ); précédent : 002643; suivant : 002645

Tropism of and innate immune responses to the novel human betacoronavirus lineage C virus in human ex vivo respiratory organ cultures.

Auteurs : Renee W Y. Chan [République populaire de Chine] ; Michael C W. Chan ; Sudhakar Agnihothram ; Louisa L Y. Chan ; Denise I T. Kuok ; Joanne H M. Fong ; Y. Guan ; Leo L M. Poon ; Ralph S. Baric ; John M. Nicholls ; J S Malik Peiris

Source :

Journal of virology [ 1098-5514 ] ; 2013.

RBID : pubmed:23552422

Descripteurs français

KwdFr :
- Bronches (cytologie), Bronches (virologie), Cellules épithéliales (virologie), Coronavirus (), Coronavirus (génétique), Coronavirus (immunologie), Coronavirus (physiologie), Coronavirus humain 229E (physiologie), Humains, Immunité innée, Infections à coronavirus (immunologie), Infections à coronavirus (virologie), Interférons (pharmacologie), Lignée cellulaire, Poumon (cytologie), Poumon (virologie), Récepteurs viraux (métabolisme), Réplication virale, Syndrome respiratoire aigu sévère (immunologie), Syndrome respiratoire aigu sévère (virologie), Techniques de culture d'organes, Tropisme viral, Virus du SRAS (physiologie).
MESH :
- cytologie : Bronches, Poumon.
- génétique : Coronavirus.
- immunologie : Coronavirus, Infections à coronavirus, Syndrome respiratoire aigu sévère.
- métabolisme : Récepteurs viraux.
- pharmacologie : Interférons.
- physiologie : Coronavirus, Coronavirus humain 229E, Virus du SRAS.
- virologie : Bronches, Cellules épithéliales, Infections à coronavirus, Poumon, Syndrome respiratoire aigu sévère.
- Coronavirus, Humains, Immunité innée, Lignée cellulaire, Réplication virale, Techniques de culture d'organes, Tropisme viral.

English descriptors

KwdEn :
- Bronchi (cytology), Bronchi (virology), Cell Line, Coronavirus (drug effects), Coronavirus (genetics), Coronavirus (immunology), Coronavirus (physiology), Coronavirus 229E, Human (physiology), Coronavirus Infections (immunology), Coronavirus Infections (virology), Epithelial Cells (virology), Humans, Immunity, Innate, Interferons (pharmacology), Lung (cytology), Lung (virology), Organ Culture Techniques, Receptors, Virus (metabolism), SARS Virus (physiology), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (virology), Viral Tropism, Virus Replication.
MESH :
- chemical , metabolism : Receptors, Virus.
- chemical , pharmacology : Interferons.
- cytology : Bronchi, Lung.
- drug effects : Coronavirus.
- genetics : Coronavirus.
- immunology : Coronavirus, Coronavirus Infections, Severe Acute Respiratory Syndrome.
- physiology : Coronavirus, Coronavirus 229E, Human, SARS Virus.
- virology : Bronchi, Coronavirus Infections, Epithelial Cells, Lung, Severe Acute Respiratory Syndrome.
- Cell Line, Humans, Immunity, Innate, Organ Culture Techniques, Viral Tropism, Virus Replication.

Abstract

Since April 2012, there have been 17 laboratory-confirmed human cases of respiratory disease associated with newly recognized human betacoronavirus lineage C virus EMC (HCoV-EMC), and 7 of them were fatal. The transmissibility and pathogenesis of HCoV-EMC remain poorly understood, and elucidating its cellular tropism in human respiratory tissues will provide mechanistic insights into the key cellular targets for virus propagation and spread. We utilized ex vivo cultures of human bronchial and lung tissue specimens to investigate the tissue tropism and virus replication kinetics following experimental infection with HCoV-EMC compared with those following infection with human coronavirus 229E (HCoV-229E) and severe acute respiratory syndrome coronavirus (SARS-CoV). The innate immune responses elicited by HCoV-EMC were also investigated. HCoV-EMC productively replicated in human bronchial and lung ex vivo organ cultures. While SARS-CoV productively replicated in lung tissue, replication in human bronchial tissue was limited. Immunohistochemistry revealed that HCoV-EMC infected nonciliated bronchial epithelium, bronchiolar epithelial cells, alveolar epithelial cells, and endothelial cells. Transmission electron microscopy showed virions within the cytoplasm of bronchial epithelial cells and budding virions from alveolar epithelial cells (type II). In contrast, there was minimal HCoV-229E infection in these tissues. HCoV-EMC failed to elicit strong type I or III interferon (IFN) or proinflammatory innate immune responses in ex vivo respiratory tissue cultures. Treatment of human lung tissue ex vivo organ cultures with type I IFNs (alpha and beta IFNs) at 1 h postinfection reduced the replication of HCoV-EMC, suggesting a potential therapeutic use of IFNs for treatment of human infection.

DOI: 10.1128/JVI.00009-13
PubMed: 23552422

Links toward previous steps (curation, corpus...)

to stream PubMed, to step Corpus: 001228
to stream PubMed, to step Curation: 001228
to stream PubMed, to step Checkpoint: 001073

Links to Exploration step

pubmed:23552422

Le document en format XML

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<front><div type="abstract" xml:lang="en">Since April 2012, there have been 17 laboratory-confirmed human cases of respiratory disease associated with newly recognized human betacoronavirus lineage C virus EMC (HCoV-EMC), and 7 of them were fatal. The transmissibility and pathogenesis of HCoV-EMC remain poorly understood, and elucidating its cellular tropism in human respiratory tissues will provide mechanistic insights into the key cellular targets for virus propagation and spread. We utilized ex vivo cultures of human bronchial and lung tissue specimens to investigate the tissue tropism and virus replication kinetics following experimental infection with HCoV-EMC compared with those following infection with human coronavirus 229E (HCoV-229E) and severe acute respiratory syndrome coronavirus (SARS-CoV). The innate immune responses elicited by HCoV-EMC were also investigated. HCoV-EMC productively replicated in human bronchial and lung ex vivo organ cultures. While SARS-CoV productively replicated in lung tissue, replication in human bronchial tissue was limited. Immunohistochemistry revealed that HCoV-EMC infected nonciliated bronchial epithelium, bronchiolar epithelial cells, alveolar epithelial cells, and endothelial cells. Transmission electron microscopy showed virions within the cytoplasm of bronchial epithelial cells and budding virions from alveolar epithelial cells (type II). In contrast, there was minimal HCoV-229E infection in these tissues. HCoV-EMC failed to elicit strong type I or III interferon (IFN) or proinflammatory innate immune responses in ex vivo respiratory tissue cultures. Treatment of human lung tissue ex vivo organ cultures with type I IFNs (alpha and beta IFNs) at 1 h postinfection reduced the replication of HCoV-EMC, suggesting a potential therapeutic use of IFNs for treatment of human infection.</div>
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<Abstract><AbstractText>Since April 2012, there have been 17 laboratory-confirmed human cases of respiratory disease associated with newly recognized human betacoronavirus lineage C virus EMC (HCoV-EMC), and 7 of them were fatal. The transmissibility and pathogenesis of HCoV-EMC remain poorly understood, and elucidating its cellular tropism in human respiratory tissues will provide mechanistic insights into the key cellular targets for virus propagation and spread. We utilized ex vivo cultures of human bronchial and lung tissue specimens to investigate the tissue tropism and virus replication kinetics following experimental infection with HCoV-EMC compared with those following infection with human coronavirus 229E (HCoV-229E) and severe acute respiratory syndrome coronavirus (SARS-CoV). The innate immune responses elicited by HCoV-EMC were also investigated. HCoV-EMC productively replicated in human bronchial and lung ex vivo organ cultures. While SARS-CoV productively replicated in lung tissue, replication in human bronchial tissue was limited. Immunohistochemistry revealed that HCoV-EMC infected nonciliated bronchial epithelium, bronchiolar epithelial cells, alveolar epithelial cells, and endothelial cells. Transmission electron microscopy showed virions within the cytoplasm of bronchial epithelial cells and budding virions from alveolar epithelial cells (type II). In contrast, there was minimal HCoV-229E infection in these tissues. HCoV-EMC failed to elicit strong type I or III interferon (IFN) or proinflammatory innate immune responses in ex vivo respiratory tissue cultures. Treatment of human lung tissue ex vivo organ cultures with type I IFNs (alpha and beta IFNs) at 1 h postinfection reduced the replication of HCoV-EMC, suggesting a potential therapeutic use of IFNs for treatment of human infection.</AbstractText>
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<MeshHeading><DescriptorName UI="D028941" MajorTopicYN="N">Coronavirus 229E, Human</DescriptorName>
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Tropism of and innate immune responses to the novel human betacoronavirus lineage C virus in human ex vivo respiratory organ cultures.

Tropism of and innate immune responses to the novel human betacoronavirus lineage C virus in human ex vivo respiratory organ cultures.

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