[Development of serological detection assays for human coronavirus HKU1 infection and its application].
Identifieur interne : 002285 ( Ncbi/Merge ); précédent : 002284; suivant : 002286[Development of serological detection assays for human coronavirus HKU1 infection and its application].
Auteurs : Wei-Min Zhou [République populaire de Chine] ; Wen-Ling Wang ; Wen-Jie Tan ; Ling-Lin Zhang ; Xiao Yin ; Rou-Jian Lu ; Hui-Juan WangSource :
- Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology [ 1003-9279 ] ; 2010.
Descripteurs français
- KwdFr :
- Anticorps antiviraux (immunologie), Anticorps antiviraux (sang), Coronavirus (génétique), Coronavirus (immunologie), Coronavirus (isolement et purification), Coronavirus (physiologie), Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (immunologie), Humains, Infections à coronavirus (diagnostic), Infections à coronavirus (immunologie), Infections à coronavirus (sang), Infections à coronavirus (virologie), Lignée cellulaire, Protéines de capside (génétique), Protéines de capside (immunologie), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (immunologie), Tests sérologiques ().
- MESH :
- diagnostic : Infections à coronavirus.
- génétique : Coronavirus, Glycoprotéines membranaires, Protéines de capside, Protéines de l'enveloppe virale.
- immunologie : Anticorps antiviraux, Coronavirus, Glycoprotéines membranaires, Infections à coronavirus, Protéines de capside, Protéines de l'enveloppe virale.
- isolement et purification : Coronavirus.
- physiologie : Coronavirus.
- sang : Anticorps antiviraux, Infections à coronavirus.
- virologie : Infections à coronavirus.
- Glycoprotéine de spicule des coronavirus, Humains, Lignée cellulaire, Tests sérologiques.
English descriptors
- KwdEn :
- Antibodies, Viral (blood), Antibodies, Viral (immunology), Capsid Proteins (genetics), Capsid Proteins (immunology), Cell Line, Coronavirus (genetics), Coronavirus (immunology), Coronavirus (isolation & purification), Coronavirus (physiology), Coronavirus Infections (blood), Coronavirus Infections (diagnosis), Coronavirus Infections (immunology), Coronavirus Infections (virology), Humans, Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Serologic Tests (methods), Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology).
- MESH :
- chemical , blood : Antibodies, Viral.
- chemical , genetics : Capsid Proteins, Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , immunology : Antibodies, Viral, Capsid Proteins, Membrane Glycoproteins, Viral Envelope Proteins.
- blood : Coronavirus Infections.
- diagnosis : Coronavirus Infections.
- genetics : Coronavirus.
- immunology : Coronavirus, Coronavirus Infections.
- isolation & purification : Coronavirus.
- methods : Serologic Tests.
- physiology : Coronavirus.
- virology : Coronavirus Infections.
- Cell Line, Humans, Spike Glycoprotein, Coronavirus.
Abstract
To express the nuclear capsid protein (N protein) and the spike protein (S protein) of HCoV-HKU1, and to develop the corresponding serum assay for antibody detection.
PubMed: 21280330
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pubmed:21280330Le document en format XML
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uniqKey="Tan W" first="Wen-Jie" last="Tan">Wen-Jie Tan</name></author><author><name sortKey="Zhang, Ling Lin" sort="Zhang, Ling Lin" uniqKey="Zhang L" first="Ling-Lin" last="Zhang">Ling-Lin Zhang</name></author><author><name sortKey="Yin, Xiao" sort="Yin, Xiao" uniqKey="Yin X" first="Xiao" last="Yin">Xiao Yin</name></author><author><name sortKey="Lu, Rou Jian" sort="Lu, Rou Jian" uniqKey="Lu R" first="Rou-Jian" last="Lu">Rou-Jian Lu</name></author><author><name sortKey="Wang, Hui Juan" sort="Wang, Hui Juan" uniqKey="Wang H" first="Hui-Juan" last="Wang">Hui-Juan Wang</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010</date><idno type="RBID">pubmed:21280330</idno><idno type="pmid">21280330</idno><idno type="wicri:Area/PubMed/Corpus">001556</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001556</idno><idno type="wicri:Area/PubMed/Curation">001556</idno><idno type="wicri:explorRef" 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type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Wang, Wen Ling" sort="Wang, Wen Ling" uniqKey="Wang W" first="Wen-Ling" last="Wang">Wen-Ling Wang</name></author><author><name sortKey="Tan, Wen Jie" sort="Tan, Wen Jie" uniqKey="Tan W" first="Wen-Jie" last="Tan">Wen-Jie Tan</name></author><author><name sortKey="Zhang, Ling Lin" sort="Zhang, Ling Lin" uniqKey="Zhang L" first="Ling-Lin" last="Zhang">Ling-Lin Zhang</name></author><author><name sortKey="Yin, Xiao" sort="Yin, Xiao" uniqKey="Yin X" first="Xiao" last="Yin">Xiao Yin</name></author><author><name sortKey="Lu, Rou Jian" sort="Lu, Rou Jian" uniqKey="Lu R" first="Rou-Jian" last="Lu">Rou-Jian Lu</name></author><author><name sortKey="Wang, Hui Juan" sort="Wang, Hui Juan" uniqKey="Wang H" first="Hui-Juan" last="Wang">Hui-Juan Wang</name></author></analytic><series><title level="j">Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology</title><idno type="ISSN">1003-9279</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibodies, Viral (blood)</term><term>Antibodies, Viral (immunology)</term><term>Capsid Proteins (genetics)</term><term>Capsid Proteins (immunology)</term><term>Cell Line</term><term>Coronavirus (genetics)</term><term>Coronavirus (immunology)</term><term>Coronavirus (isolation & purification)</term><term>Coronavirus (physiology)</term><term>Coronavirus Infections (blood)</term><term>Coronavirus Infections (diagnosis)</term><term>Coronavirus Infections (immunology)</term><term>Coronavirus Infections (virology)</term><term>Humans</term><term>Membrane Glycoproteins (genetics)</term><term>Membrane Glycoproteins (immunology)</term><term>Serologic Tests (methods)</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Envelope Proteins (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Anticorps antiviraux (immunologie)</term><term>Anticorps antiviraux (sang)</term><term>Coronavirus (génétique)</term><term>Coronavirus (immunologie)</term><term>Coronavirus (isolement et purification)</term><term>Coronavirus (physiologie)</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (génétique)</term><term>Glycoprotéines membranaires (immunologie)</term><term>Humains</term><term>Infections à coronavirus (diagnostic)</term><term>Infections à coronavirus (immunologie)</term><term>Infections à coronavirus (sang)</term><term>Infections à coronavirus (virologie)</term><term>Lignée cellulaire</term><term>Protéines de capside (génétique)</term><term>Protéines de capside (immunologie)</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Tests sérologiques ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Capsid Proteins</term><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Viral</term><term>Capsid Proteins</term><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="blood" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Coronavirus</term><term>Glycoprotéines membranaires</term><term>Protéines de capside</term><term>Protéines de l'enveloppe virale</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiviraux</term><term>Coronavirus</term><term>Glycoprotéines membranaires</term><term>Infections à coronavirus</term><term>Protéines de capside</term><term>Protéines de l'enveloppe virale</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Coronavirus</term><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Serologic Tests</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cell Line</term><term>Humans</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Glycoprotéine de spicule des coronavirus</term><term>Humains</term><term>Lignée cellulaire</term><term>Tests sérologiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To express the nuclear capsid protein (N protein) and the spike protein (S protein) of HCoV-HKU1, and to develop the corresponding serum assay for antibody detection.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21280330</PMID><DateCompleted><Year>2011</Year><Month>02</Month><Day>24</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1003-9279</ISSN><JournalIssue CitedMedium="Print"><Volume>24</Volume><Issue>5</Issue><PubDate><Year>2010</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology</Title><ISOAbbreviation>Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi</ISOAbbreviation></Journal><ArticleTitle>[Development of serological detection assays for human coronavirus HKU1 infection and its application].</ArticleTitle><Pagination><MedlinePgn>376-9</MedlinePgn></Pagination><Abstract><AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">To express the nuclear capsid protein (N protein) and the spike protein (S protein) of HCoV-HKU1, and to develop the corresponding serum assay for antibody detection.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">The N protein of HCoV-HKU1 was expressed in E. Coli, anti-N antibody assay was established using Western Blotting with turn-based membrane. HCoV-HKU1 S protein was constructed in the eukaryotic expression plasmids, and confirmed by Western Blotting, S antibody assay was established using indirect immunofluorescence assay (IFA). We analyzed anti-S and anti-N antibody among 100 normal adult serum.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">Expression of S and N protein were confirmed; 100 normal adult serum were analyzed using the established serological detection assay, in which HCoV-HKU1 S antibody positive rate was 47%, N antibody positive rate was 48%, Both S and N antibodies positive were 21%, Both S and N antibodies negative were 22%. Co-detection S and N antibody was achieved 74% positive rate.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">The methods we established here could be used for serological analysis of HCoV-HKU1. Either detection of HCoV-HKU1 S or N antibodies achieved good results. Higher positive detection rate of anti-S or anti-N antibody was found in the normal adults.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhou</LastName><ForeName>Wei-min</ForeName><Initials>WM</Initials><AffiliationInfo><Affiliation>National Institute for Viral Disease Control and Prevention, China Center for Disease Control and Prevention, Beijing 100052, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Wen-ling</ForeName><Initials>WL</Initials></Author><Author ValidYN="Y"><LastName>Tan</LastName><ForeName>Wen-jie</ForeName><Initials>WJ</Initials></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Ling-lin</ForeName><Initials>LL</Initials></Author><Author ValidYN="Y"><LastName>Yin</LastName><ForeName>Xiao</ForeName><Initials>X</Initials></Author><Author ValidYN="Y"><LastName>Lu</LastName><ForeName>Rou-jian</ForeName><Initials>RJ</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Hui-juan</ForeName><Initials>HJ</Initials></Author></AuthorList><Language>chi</Language><PublicationTypeList><PublicationType UI="D023362">Evaluation Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi</MedlineTA><NlmUniqueID>9602873</NlmUniqueID><ISSNLinking>1003-9279</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000914">Antibodies, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D036022">Capsid Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C578553">MHV surface projection glycoprotein</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008562">Membrane Glycoproteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D064370">Spike Glycoprotein, Coronavirus</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014759">Viral Envelope Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C578557">spike glycoprotein, SARS-CoV</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000914" MajorTopicYN="N">Antibodies, Viral</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="N">blood</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D036022" MajorTopicYN="N">Capsid Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002460" MajorTopicYN="N">Cell Line</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017934" MajorTopicYN="N">Coronavirus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018352" MajorTopicYN="N">Coronavirus Infections</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="N">blood</QualifierName><QualifierName UI="Q000175" MajorTopicYN="N">diagnosis</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008562" MajorTopicYN="N">Membrane Glycoproteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012698" MajorTopicYN="N">Serologic Tests</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D064370" MajorTopicYN="N">Spike Glycoprotein, Coronavirus</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014759" MajorTopicYN="N">Viral Envelope Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2011</Year><Month>2</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2011</Year><Month>2</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2011</Year><Month>2</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">21280330</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><noCountry><name sortKey="Lu, Rou Jian" sort="Lu, Rou Jian" uniqKey="Lu R" first="Rou-Jian" last="Lu">Rou-Jian Lu</name><name sortKey="Tan, Wen Jie" sort="Tan, Wen Jie" uniqKey="Tan W" first="Wen-Jie" last="Tan">Wen-Jie Tan</name><name sortKey="Wang, Hui Juan" sort="Wang, Hui Juan" uniqKey="Wang H" first="Hui-Juan" last="Wang">Hui-Juan Wang</name><name sortKey="Wang, Wen Ling" sort="Wang, Wen Ling" uniqKey="Wang W" first="Wen-Ling" last="Wang">Wen-Ling Wang</name><name sortKey="Yin, Xiao" sort="Yin, Xiao" uniqKey="Yin X" first="Xiao" last="Yin">Xiao Yin</name><name sortKey="Zhang, Ling Lin" sort="Zhang, Ling Lin" uniqKey="Zhang L" first="Ling-Lin" last="Zhang">Ling-Lin Zhang</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Zhou, Wei Min" sort="Zhou, Wei Min" uniqKey="Zhou W" first="Wei-Min" last="Zhou">Wei-Min Zhou</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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