Translational control of the subgenomic RNAs of severe acute respiratory syndrome coronavirus.
Identifieur interne : 001E88 ( Ncbi/Merge ); précédent : 001E87; suivant : 001E89Translational control of the subgenomic RNAs of severe acute respiratory syndrome coronavirus.
Auteurs : Yaling Yang [République populaire de Chine] ; Snawar Hussain ; Hao Wang ; Min Ke ; Deyin GuoSource :
- Virus genes [ 1572-994X ] ; 2009.
Descripteurs français
- KwdFr :
- ARN viral (métabolisme), Animaux, Biosynthèse des protéines, Codon d'initiation, Cricetinae, Gènes rapporteurs, Lignée cellulaire, Luciferases (métabolisme), Protéines à fluorescence verte (métabolisme), Régions 5' non traduites, Régulation de l'expression des gènes viraux, Virus du SRAS (physiologie).
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Green Fluorescent Proteins, Luciferases, RNA, Viral.
- chemical : 5' Untranslated Regions, Codon, Initiator.
- physiology : SARS Virus.
- Animals, Cell Line, Cricetinae, Gene Expression Regulation, Viral, Genes, Reporter, Protein Biosynthesis.
Abstract
The 3'-one-third of the severe acute respiratory syndrome coronavirus (SARS-CoV) genome contains genes for four essential structural proteins and eight virus-specific genes. The expression of this genomic information of SARS-CoV involves synthesis of a nested set of subgenomic RNAs (sgRNAs). In this study, we showed that the translational levels of 10 SARS-CoV sgRNAs including the two low-abundance sgRNAs 2-1 and 3-1 varied considerably in translation reporter assays. We also demonstrated that the initiator AUG codon of sgRNA-8 was silent and the repressive control was most likely positioned in the upstream untranslated region (UTR) of sgRNA-8. The initiator AUG codons of most sgRNAs are in poor Kozak contexts and the translation of truncated proteins from downstream AUG codons by leaky scanning was common in our experimental settings. No significant correlation was found between complexity of 5'-UTR and the sequence context of AUG codon with the level of translation of SARS-CoV sgRNAs. These results will be helpful for further studies to reveal the biological functions and translation regulatory mechanisms of sgRNAs in the coronavirus life cycle and pathogenesis.
DOI: 10.1007/s11262-009-0357-y
PubMed: 19363699
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type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>5' Untranslated Regions</term><term>Animals</term><term>Cell Line</term><term>Codon, Initiator</term><term>Cricetinae</term><term>Gene Expression Regulation, Viral</term><term>Genes, Reporter</term><term>Green Fluorescent Proteins (metabolism)</term><term>Luciferases (metabolism)</term><term>Protein Biosynthesis</term><term>RNA, Viral (metabolism)</term><term>SARS Virus (physiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (métabolisme)</term><term>Animaux</term><term>Biosynthèse des protéines</term><term>Codon d'initiation</term><term>Cricetinae</term><term>Gènes rapporteurs</term><term>Lignée cellulaire</term><term>Luciferases (métabolisme)</term><term>Protéines à fluorescence verte (métabolisme)</term><term>Régions 5' non traduites</term><term>Régulation de l'expression des gènes viraux</term><term>Virus du SRAS (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Green Fluorescent Proteins</term><term>Luciferases</term><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>5' Untranslated Regions</term><term>Codon, Initiator</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN viral</term><term>Luciferases</term><term>Protéines à fluorescence verte</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Cricetinae</term><term>Gene Expression Regulation, Viral</term><term>Genes, Reporter</term><term>Protein Biosynthesis</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Biosynthèse des protéines</term><term>Codon d'initiation</term><term>Cricetinae</term><term>Gènes rapporteurs</term><term>Lignée cellulaire</term><term>Régions 5' non traduites</term><term>Régulation de l'expression des gènes viraux</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The 3'-one-third of the severe acute respiratory syndrome coronavirus (SARS-CoV) genome contains genes for four essential structural proteins and eight virus-specific genes. The expression of this genomic information of SARS-CoV involves synthesis of a nested set of subgenomic RNAs (sgRNAs). In this study, we showed that the translational levels of 10 SARS-CoV sgRNAs including the two low-abundance sgRNAs 2-1 and 3-1 varied considerably in translation reporter assays. We also demonstrated that the initiator AUG codon of sgRNA-8 was silent and the repressive control was most likely positioned in the upstream untranslated region (UTR) of sgRNA-8. The initiator AUG codons of most sgRNAs are in poor Kozak contexts and the translation of truncated proteins from downstream AUG codons by leaky scanning was common in our experimental settings. No significant correlation was found between complexity of 5'-UTR and the sequence context of AUG codon with the level of translation of SARS-CoV sgRNAs. These results will be helpful for further studies to reveal the biological functions and translation regulatory mechanisms of sgRNAs in the coronavirus life cycle and pathogenesis.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19363699</PMID><DateCompleted><Year>2009</Year><Month>09</Month><Day>08</Day></DateCompleted><DateRevised><Year>2020</Year><Month>03</Month><Day>24</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1572-994X</ISSN><JournalIssue CitedMedium="Internet"><Volume>39</Volume><Issue>1</Issue><PubDate><Year>2009</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Virus genes</Title><ISOAbbreviation>Virus Genes</ISOAbbreviation></Journal><ArticleTitle>Translational control of the subgenomic RNAs of severe acute respiratory syndrome coronavirus.</ArticleTitle><Pagination><MedlinePgn>10-8</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s11262-009-0357-y</ELocationID><Abstract><AbstractText>The 3'-one-third of the severe acute respiratory syndrome coronavirus (SARS-CoV) genome contains genes for four essential structural proteins and eight virus-specific genes. The expression of this genomic information of SARS-CoV involves synthesis of a nested set of subgenomic RNAs (sgRNAs). In this study, we showed that the translational levels of 10 SARS-CoV sgRNAs including the two low-abundance sgRNAs 2-1 and 3-1 varied considerably in translation reporter assays. We also demonstrated that the initiator AUG codon of sgRNA-8 was silent and the repressive control was most likely positioned in the upstream untranslated region (UTR) of sgRNA-8. The initiator AUG codons of most sgRNAs are in poor Kozak contexts and the translation of truncated proteins from downstream AUG codons by leaky scanning was common in our experimental settings. No significant correlation was found between complexity of 5'-UTR and the sequence context of AUG codon with the level of translation of SARS-CoV sgRNAs. These results will be helpful for further studies to reveal the biological functions and translation regulatory mechanisms of sgRNAs in the coronavirus life cycle and pathogenesis.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Yaling</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Virology and the Modern Virology Research Centre, College of Life Sciences, Wuhan University, Wuhan 430072, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hussain</LastName><ForeName>Snawar</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Hao</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Ke</LastName><ForeName>Min</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Guo</LastName><ForeName>Deyin</ForeName><Initials>D</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2009</Year><Month>04</Month><Day>12</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Virus Genes</MedlineTA><NlmUniqueID>8803967</NlmUniqueID><ISSNLinking>0920-8569</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D020121">5' Untranslated Regions</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018387">Codon, Initiator</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012367">RNA, 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IdType="pubmed">15744567</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Hubei</li></region><settlement><li>Wuhan</li></settlement><orgName><li>Université de Wuhan</li></orgName></list><tree><noCountry><name sortKey="Guo, Deyin" sort="Guo, Deyin" uniqKey="Guo D" first="Deyin" last="Guo">Deyin Guo</name><name sortKey="Hussain, Snawar" sort="Hussain, Snawar" uniqKey="Hussain S" first="Snawar" last="Hussain">Snawar Hussain</name><name sortKey="Ke, Min" sort="Ke, Min" uniqKey="Ke M" first="Min" last="Ke">Min Ke</name><name sortKey="Wang, Hao" sort="Wang, Hao" uniqKey="Wang H" first="Hao" last="Wang">Hao Wang</name></noCountry><country name="République populaire de Chine"><region name="Hubei"><name sortKey="Yang, Yaling" sort="Yang, Yaling" uniqKey="Yang Y" first="Yaling" last="Yang">Yaling Yang</name></region></country></tree></affiliations></record>Pour manipuler ce 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