Rapid and real-time detection technologies for emerging viruses of biomedical importance
Identifieur interne : 001E47 ( Ncbi/Merge ); précédent : 001E46; suivant : 001E48Rapid and real-time detection technologies for emerging viruses of biomedical importance
Auteurs : M. M. ParidaSource :
- Journal of Biosciences [ 0250-5991 ] ; 2008.
Descripteurs français
- KwdFr :
- ADN viral (), ARN viral (), Amorces ADN, Humains, Maladies transmissibles émergentes (diagnostic), Maladies transmissibles émergentes (virologie), Maladies virales (diagnostic), RT-PCR, Sensibilité et spécificité, Sérotypage (), Techniques d'amplification d'acides nucléiques, Techniques et procédures diagnostiques, Virus (génétique), Virus (isolement et purification).
- MESH :
- diagnostic : Maladies transmissibles émergentes, Maladies virales.
- génétique : Virus.
- isolement et purification : Virus.
- virologie : Maladies transmissibles émergentes.
- ADN viral, ARN viral, Amorces ADN, Humains, RT-PCR, Sensibilité et spécificité, Sérotypage, Techniques d'amplification d'acides nucléiques, Techniques et procédures diagnostiques.
English descriptors
- KwdEn :
- Communicable Diseases, Emerging (diagnosis), Communicable Diseases, Emerging (virology), DNA Primers, DNA, Viral (chemistry), Diagnostic Techniques and Procedures, Humans, Nucleic Acid Amplification Techniques, RNA, Viral (chemistry), Reverse Transcriptase Polymerase Chain Reaction, Sensitivity and Specificity, Serotyping (methods), Virus Diseases (diagnosis), Viruses (genetics), Viruses (isolation & purification).
- MESH :
- chemical , chemistry : DNA, Viral, RNA, Viral.
- chemical : DNA Primers.
- diagnosis : Communicable Diseases, Emerging, Virus Diseases.
- genetics : Viruses.
- isolation & purification : Viruses.
- methods : Serotyping.
- virology : Communicable Diseases, Emerging.
- Diagnostic Techniques and Procedures, Humans, Nucleic Acid Amplification Techniques, Reverse Transcriptase Polymerase Chain Reaction, Sensitivity and Specificity.
Abstract
The development of technologies with rapid and sensitive detection capabilities and increased throughput have become crucial for responding to greater number threats posed by emerging and re-emerging viruses in the recent past. The conventional identification methods require time-consuming culturing, and/ or detection of antibodies, which are not very sensitive and specific. The recent advances in molecular biology techniques in the field of genomics and proteomics greatly facilitate the rapid identification with more accuracy. We have developed two real-time assays ie., SYBR green I based real time reverse transcription polymerase chain reaction (RT-PCR) and RT-loop-mediated isothermal amplification (LAMP) assay for rapid detection as well as typing of some of the emerging viruses of biomedical importance viz. dengue, Japanese encephalitis, chikungunya, west Nile, severe acute respiratory syndrome virus (SARS) etc. Both these techniques are capable of detection and differentiation as well as quantifying viral load with higher sensitivity, rapidity, specificity. One of the most important advantages of LAMP is its field applicability, without requirement of any sophisticated equipments. Both these assays have been extensively evaluated and validated with clinical samples of recent epidemics from different parts of India. The establishment of these real time molecular assays will certainly facilitate the rapid detection of viruses with high degree of precision and accuracy in future.
Url:
DOI: 10.1007/s12038-008-0079-7
PubMed: 19208986
PubMed Central: 7090734
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PMC:7090734Le document en format XML
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M." last="Parida">M. M. Parida</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author></analytic><series><title level="j">Journal of Biosciences</title><idno type="ISSN">0250-5991</idno><idno type="eISSN">0973-7138</idno><imprint><date when="2008">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Communicable Diseases, Emerging (diagnosis)</term><term>Communicable Diseases, Emerging (virology)</term><term>DNA Primers</term><term>DNA, Viral (chemistry)</term><term>Diagnostic Techniques and Procedures</term><term>Humans</term><term>Nucleic Acid Amplification Techniques</term><term>RNA, Viral (chemistry)</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Sensitivity and Specificity</term><term>Serotyping (methods)</term><term>Virus Diseases (diagnosis)</term><term>Viruses (genetics)</term><term>Viruses (isolation & purification)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN viral ()</term><term>ARN viral ()</term><term>Amorces ADN</term><term>Humains</term><term>Maladies transmissibles émergentes (diagnostic)</term><term>Maladies transmissibles émergentes (virologie)</term><term>Maladies virales (diagnostic)</term><term>RT-PCR</term><term>Sensibilité et spécificité</term><term>Sérotypage ()</term><term>Techniques d'amplification d'acides nucléiques</term><term>Techniques et procédures diagnostiques</term><term>Virus (génétique)</term><term>Virus (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA, Viral</term><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA Primers</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Communicable Diseases, Emerging</term><term>Virus Diseases</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Maladies transmissibles émergentes</term><term>Maladies virales</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Viruses</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Virus</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Viruses</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Serotyping</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Maladies transmissibles émergentes</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Communicable Diseases, Emerging</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Diagnostic Techniques and Procedures</term><term>Humans</term><term>Nucleic Acid Amplification Techniques</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ADN viral</term><term>ARN viral</term><term>Amorces ADN</term><term>Humains</term><term>RT-PCR</term><term>Sensibilité et spécificité</term><term>Sérotypage</term><term>Techniques d'amplification d'acides nucléiques</term><term>Techniques et procédures diagnostiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The development of technologies with rapid and sensitive detection capabilities and increased throughput have become crucial for responding to greater number threats posed by emerging and re-emerging viruses in the recent past. The conventional identification methods require time-consuming culturing, and/ or detection of antibodies, which are not very sensitive and specific. The recent advances in molecular biology techniques in the field of genomics and proteomics greatly facilitate the rapid identification with more accuracy. We have developed two real-time assays ie., SYBR green I based real time reverse transcription polymerase chain reaction (RT-PCR) and RT-loop-mediated isothermal amplification (LAMP) assay for rapid detection as well as typing of some of the emerging viruses of biomedical importance viz. dengue, Japanese encephalitis, chikungunya, west Nile, severe acute respiratory syndrome virus (SARS) etc. Both these techniques are capable of detection and differentiation as well as quantifying viral load with higher sensitivity, rapidity, specificity. One of the most important advantages of LAMP is its field applicability, without requirement of any sophisticated equipments. Both these assays have been extensively evaluated and validated with clinical samples of recent epidemics from different parts of India. The establishment of these real time molecular assays will certainly facilitate the rapid detection of viruses with high degree of precision and accuracy in future.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Beuret, C" uniqKey="Beuret C">C. Beuret</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chan, A B" uniqKey="Chan A">A. B. Chan</name></author><author><name sortKey="Fox, J D" uniqKey="Fox J">J. D. Fox</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chien, L J" uniqKey="Chien L">L. J. Chien</name></author><author><name sortKey="Liao, T L" uniqKey="Liao T">T. L. Liao</name></author><author><name sortKey="Shu, P Y" uniqKey="Shu P">P. Y. 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Lion</name></author></analytic></biblStruct></listBibl></div1></back></TEI><double pmid="19208986"><pmc><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Rapid and real-time detection technologies for emerging viruses of biomedical importance</title><author><name sortKey="Parida, M M" sort="Parida, M M" uniqKey="Parida M" first="M. M." last="Parida">M. M. Parida</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">19208986</idno><idno type="pmc">7090734</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7090734</idno><idno type="RBID">PMC:7090734</idno><idno type="doi">10.1007/s12038-008-0079-7</idno><date when="2008">2008</date><idno type="wicri:Area/Pmc/Corpus">000410</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000410</idno><idno type="wicri:Area/Pmc/Curation">000410</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000410</idno><idno type="wicri:Area/Pmc/Checkpoint">000D92</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000D92</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Rapid and real-time detection technologies for emerging viruses of biomedical importance</title><author><name sortKey="Parida, M M" sort="Parida, M M" uniqKey="Parida M" first="M. M." last="Parida">M. M. Parida</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author></analytic><series><title level="j">Journal of Biosciences</title><idno type="ISSN">0250-5991</idno><idno type="eISSN">0973-7138</idno><imprint><date when="2008">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The development of technologies with rapid and sensitive detection capabilities and increased throughput have become crucial for responding to greater number threats posed by emerging and re-emerging viruses in the recent past. The conventional identification methods require time-consuming culturing, and/ or detection of antibodies, which are not very sensitive and specific. The recent advances in molecular biology techniques in the field of genomics and proteomics greatly facilitate the rapid identification with more accuracy. We have developed two real-time assays ie., SYBR green I based real time reverse transcription polymerase chain reaction (RT-PCR) and RT-loop-mediated isothermal amplification (LAMP) assay for rapid detection as well as typing of some of the emerging viruses of biomedical importance viz. dengue, Japanese encephalitis, chikungunya, west Nile, severe acute respiratory syndrome virus (SARS) etc. Both these techniques are capable of detection and differentiation as well as quantifying viral load with higher sensitivity, rapidity, specificity. One of the most important advantages of LAMP is its field applicability, without requirement of any sophisticated equipments. Both these assays have been extensively evaluated and validated with clinical samples of recent epidemics from different parts of India. 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Lion</name></author></analytic></biblStruct></listBibl></div1></back></TEI></pmc><pubmed><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Rapid and real-time detection technologies for emerging viruses of biomedical importance.</title><author><name sortKey="Parida, M M" sort="Parida, M M" uniqKey="Parida M" first="M M" last="Parida">M M Parida</name><affiliation wicri:level="1"><nlm:affiliation>Department of Virology, Defence R and D Establishment, Gwalior 474 002, India. paridamm@rediffmail.com</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Virology, Defence R and D Establishment, Gwalior 474 002</wicri:regionArea><wicri:noRegion>Gwalior 474 002</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2008">2008</date><idno type="RBID">pubmed:19208986</idno><idno type="pmid">19208986</idno><idno type="doi">10.1007/s12038-008-0079-7</idno><idno type="wicri:Area/PubMed/Corpus">001963</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001963</idno><idno type="wicri:Area/PubMed/Curation">001963</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001963</idno><idno type="wicri:Area/PubMed/Checkpoint">001A52</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001A52</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Rapid and real-time detection technologies for emerging viruses of biomedical importance.</title><author><name sortKey="Parida, M M" sort="Parida, M M" uniqKey="Parida M" first="M M" last="Parida">M M Parida</name><affiliation wicri:level="1"><nlm:affiliation>Department of Virology, Defence R and D Establishment, Gwalior 474 002, India. paridamm@rediffmail.com</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Virology, Defence R and D Establishment, Gwalior 474 002</wicri:regionArea><wicri:noRegion>Gwalior 474 002</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of biosciences</title><idno type="ISSN">0250-5991</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Communicable Diseases, Emerging (diagnosis)</term><term>Communicable Diseases, Emerging (virology)</term><term>DNA Primers</term><term>DNA, Viral (chemistry)</term><term>Diagnostic Techniques and Procedures</term><term>Humans</term><term>Nucleic Acid Amplification Techniques</term><term>RNA, Viral (chemistry)</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Sensitivity and Specificity</term><term>Serotyping (methods)</term><term>Virus Diseases (diagnosis)</term><term>Viruses (genetics)</term><term>Viruses (isolation & purification)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN viral ()</term><term>ARN viral ()</term><term>Amorces ADN</term><term>Humains</term><term>Maladies transmissibles émergentes (diagnostic)</term><term>Maladies transmissibles émergentes (virologie)</term><term>Maladies virales (diagnostic)</term><term>RT-PCR</term><term>Sensibilité et spécificité</term><term>Sérotypage ()</term><term>Techniques d'amplification d'acides nucléiques</term><term>Techniques et procédures diagnostiques</term><term>Virus (génétique)</term><term>Virus (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA, Viral</term><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA Primers</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Communicable Diseases, Emerging</term><term>Virus Diseases</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Maladies transmissibles émergentes</term><term>Maladies virales</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Viruses</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Virus</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Viruses</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Serotyping</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Maladies transmissibles émergentes</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Communicable Diseases, Emerging</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Diagnostic Techniques and Procedures</term><term>Humans</term><term>Nucleic Acid Amplification Techniques</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ADN viral</term><term>ARN viral</term><term>Amorces ADN</term><term>Humains</term><term>RT-PCR</term><term>Sensibilité et spécificité</term><term>Sérotypage</term><term>Techniques d'amplification d'acides nucléiques</term><term>Techniques et procédures diagnostiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The development of technologies with rapid and sensitive detection capabilities and increased throughput have become crucial for responding to greater number threats posed by emerging and re-emerging viruses in the recent past. The conventional identification methods require time-consuming culturing, and/or detection of antibodies,which are not very sensitive and specific. The recent advances in molecular biology techniques in the field of genomics and proteomics greatly facilitate the rapid identification with more accuracy. We have developed two real-time assays ie., SYBR green I based real time reverse transcription polymerase chain reaction (RT-PCR) and RT-loop-mediated isothermal amplification (LAMP) assay for rapid detection as well as typing of some of the emerging viruses of biomedical importance viz. dengue, Japanese encephalitis, chikungunya, west Nile, severe acute respiratory syndrome virus (SARS) etc. Both these techniques are capable of detection and differentiation as well as quantifying viral load with higher sensitivity, rapidity, specificity. One of the most important advantages of LAMP is its field applicability, without requirement of any sophisticated equipments. Both these assays have been extensively evaluated and validated with clinical samples of recent epidemics from different parts of India. The establishment of these real time molecular assays will certainly facilitate the rapid detection of viruses with high degree of precision and accuracy in future.</div></front></TEI></pubmed></double></record>Pour manipuler ce document sous Unix (Dilib)
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