SrasV1, Ncbi, Merge, bibRecord, 001E28

Peptide nanoparticles as novel immunogens: design and analysis of a prototypic severe acute respiratory syndrome vaccine.

Identifieur interne : 001E28 ( Ncbi/Merge ); précédent : 001E27; suivant : 001E29

Peptide nanoparticles as novel immunogens: design and analysis of a prototypic severe acute respiratory syndrome vaccine.

Auteurs : Tais A P F. Pimentel [États-Unis] ; Zhe Yan ; Scott A. Jeffers ; Kathryn V. Holmes ; Robert S. Hodges ; Peter Burkhard

Source :

Chemical biology & drug design [ 1747-0285 ] ; 2009.

RBID : pubmed:19152635

Descripteurs français

English descriptors

KwdEn :
- Amino Acid Sequence, Animals, Antibodies (immunology), Enzyme-Linked Immunosorbent Assay (methods), Epitopes (chemistry), Epitopes (immunology), Humans, Mice, Mice, Inbred BALB C, Models, Molecular, Molecular Sequence Data, Nanoparticles (chemistry), Nanoparticles (ultrastructure), Peptides (chemistry), Peptides (genetics), Peptides (immunology), Protein Conformation, Protein Subunits (chemistry), Protein Subunits (immunology), Recombinant Fusion Proteins (chemistry), Recombinant Fusion Proteins (genetics), Recombinant Fusion Proteins (metabolism), SARS Virus (immunology), Sequence Homology, Amino Acid, Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (prevention & control), Vaccines (chemistry), Vaccines (immunology).
MESH :
- chemical , chemistry : Epitopes, Peptides, Protein Subunits, Recombinant Fusion Proteins, Vaccines.
- chemical , genetics : Peptides, Recombinant Fusion Proteins.
- chemical , immunology : Antibodies, Epitopes, Peptides, Protein Subunits, Vaccines.
- chemistry : Nanoparticles.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome.
- chemical , metabolism : Recombinant Fusion Proteins.
- methods : Enzyme-Linked Immunosorbent Assay.
- prevention & control : Severe Acute Respiratory Syndrome.
- ultrastructure : Nanoparticles.
- Amino Acid Sequence, Animals, Humans, Mice, Mice, Inbred BALB C, Models, Molecular, Molecular Sequence Data, Protein Conformation, Sequence Homology, Amino Acid.

Abstract

Severe acute respiratory syndrome (SARS) is an infectious disease caused by a novel coronavirus that cost nearly 800 lives. While there have been no recent outbreaks of the disease, the threat remains as SARS coronavirus (SARS-CoV) like strains still exist in animal reservoirs. Therefore, the development of a vaccine against SARS is in grave need. Here, we have designed and produced a prototypic SARS vaccine: a self-assembling polypeptide nanoparticle that repetitively displays a SARS B-cell epitope from the C-terminal heptad repeat of the virus' spike protein. Biophysical analyses with circular dichroism, transmission electron microscopy and dynamic light scattering confirmed the computational design showing alpha-helcial nanoparticles with sizes of about 25 nm. Immunization experiments with no adjuvants were performed with BALB/c mice. An investigation of the binding properties of the elicited antibodies showed that they were highly conformation specific for the coiled-coil epitope because they specifically recognized the native trimeric conformation of C-terminal heptad repeat region. Consequently, the antisera exhibited neutralization activity in an in vitro infection inhibition assay. We conclude that these peptide nanoparticles represent a promising platform for vaccine design, in particular for diseases that are characterized by neutralizing epitopes with coiled-coil conformation such as SARS-CoV or other enveloped viruses.

DOI: 10.1111/j.1747-0285.2008.00746.x
PubMed: 19152635

Links toward previous steps (curation, corpus...)

to stream PubMed, to step Corpus: 001980
to stream PubMed, to step Curation: 001980
to stream PubMed, to step Checkpoint: 001821

Links to Exploration step

pubmed:19152635

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Peptide nanoparticles as novel immunogens: design and analysis of a prototypic severe acute respiratory syndrome vaccine.</title>
<author><name sortKey="Pimentel, Tais A P F" sort="Pimentel, Tais A P F" uniqKey="Pimentel T" first="Tais A P F" last="Pimentel">Tais A P F. Pimentel</name>
<affiliation wicri:level="2"><nlm:affiliation>Institute of Materials Science, University of Connecticut, Storrs, CT 06269-3136, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Institute of Materials Science, University of Connecticut, Storrs, CT 06269-3136</wicri:regionArea>
<placeName><region type="state">Connecticut</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Yan, Zhe" sort="Yan, Zhe" uniqKey="Yan Z" first="Zhe" last="Yan">Zhe Yan</name>
</author>
<author><name sortKey="Jeffers, Scott A" sort="Jeffers, Scott A" uniqKey="Jeffers S" first="Scott A" last="Jeffers">Scott A. Jeffers</name>
</author>
<author><name sortKey="Holmes, Kathryn V" sort="Holmes, Kathryn V" uniqKey="Holmes K" first="Kathryn V" last="Holmes">Kathryn V. Holmes</name>
</author>
<author><name sortKey="Hodges, Robert S" sort="Hodges, Robert S" uniqKey="Hodges R" first="Robert S" last="Hodges">Robert S. Hodges</name>
</author>
<author><name sortKey="Burkhard, Peter" sort="Burkhard, Peter" uniqKey="Burkhard P" first="Peter" last="Burkhard">Peter Burkhard</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2009">2009</date>
<idno type="RBID">pubmed:19152635</idno>
<idno type="pmid">19152635</idno>
<idno type="doi">10.1111/j.1747-0285.2008.00746.x</idno>
<idno type="wicri:Area/PubMed/Corpus">001980</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001980</idno>
<idno type="wicri:Area/PubMed/Curation">001980</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001980</idno>
<idno type="wicri:Area/PubMed/Checkpoint">001821</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001821</idno>
<idno type="wicri:Area/Ncbi/Merge">001E28</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Peptide nanoparticles as novel immunogens: design and analysis of a prototypic severe acute respiratory syndrome vaccine.</title>
<author><name sortKey="Pimentel, Tais A P F" sort="Pimentel, Tais A P F" uniqKey="Pimentel T" first="Tais A P F" last="Pimentel">Tais A P F. Pimentel</name>
<affiliation wicri:level="2"><nlm:affiliation>Institute of Materials Science, University of Connecticut, Storrs, CT 06269-3136, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Institute of Materials Science, University of Connecticut, Storrs, CT 06269-3136</wicri:regionArea>
<placeName><region type="state">Connecticut</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Yan, Zhe" sort="Yan, Zhe" uniqKey="Yan Z" first="Zhe" last="Yan">Zhe Yan</name>
</author>
<author><name sortKey="Jeffers, Scott A" sort="Jeffers, Scott A" uniqKey="Jeffers S" first="Scott A" last="Jeffers">Scott A. Jeffers</name>
</author>
<author><name sortKey="Holmes, Kathryn V" sort="Holmes, Kathryn V" uniqKey="Holmes K" first="Kathryn V" last="Holmes">Kathryn V. Holmes</name>
</author>
<author><name sortKey="Hodges, Robert S" sort="Hodges, Robert S" uniqKey="Hodges R" first="Robert S" last="Hodges">Robert S. Hodges</name>
</author>
<author><name sortKey="Burkhard, Peter" sort="Burkhard, Peter" uniqKey="Burkhard P" first="Peter" last="Burkhard">Peter Burkhard</name>
</author>
</analytic>
<series><title level="j">Chemical biology & drug design</title>
<idno type="eISSN">1747-0285</idno>
<imprint><date when="2009" type="published">2009</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Antibodies (immunology)</term>
<term>Enzyme-Linked Immunosorbent Assay (methods)</term>
<term>Epitopes (chemistry)</term>
<term>Epitopes (immunology)</term>
<term>Humans</term>
<term>Mice</term>
<term>Mice, Inbred BALB C</term>
<term>Models, Molecular</term>
<term>Molecular Sequence Data</term>
<term>Nanoparticles (chemistry)</term>
<term>Nanoparticles (ultrastructure)</term>
<term>Peptides (chemistry)</term>
<term>Peptides (genetics)</term>
<term>Peptides (immunology)</term>
<term>Protein Conformation</term>
<term>Protein Subunits (chemistry)</term>
<term>Protein Subunits (immunology)</term>
<term>Recombinant Fusion Proteins (chemistry)</term>
<term>Recombinant Fusion Proteins (genetics)</term>
<term>Recombinant Fusion Proteins (metabolism)</term>
<term>SARS Virus (immunology)</term>
<term>Sequence Homology, Amino Acid</term>
<term>Severe Acute Respiratory Syndrome (immunology)</term>
<term>Severe Acute Respiratory Syndrome (prevention & control)</term>
<term>Vaccines (chemistry)</term>
<term>Vaccines (immunology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Anticorps (immunologie)</term>
<term>Conformation des protéines</term>
<term>Données de séquences moléculaires</term>
<term>Humains</term>
<term>Modèles moléculaires</term>
<term>Nanoparticules ()</term>
<term>Nanoparticules (ultrastructure)</term>
<term>Peptides ()</term>
<term>Peptides (génétique)</term>
<term>Peptides (immunologie)</term>
<term>Protéines de fusion recombinantes ()</term>
<term>Protéines de fusion recombinantes (génétique)</term>
<term>Protéines de fusion recombinantes (métabolisme)</term>
<term>Similitude de séquences d'acides aminés</term>
<term>Souris</term>
<term>Souris de lignée BALB C</term>
<term>Sous-unités de protéines ()</term>
<term>Sous-unités de protéines (immunologie)</term>
<term>Syndrome respiratoire aigu sévère ()</term>
<term>Syndrome respiratoire aigu sévère (immunologie)</term>
<term>Séquence d'acides aminés</term>
<term>Test ELISA ()</term>
<term>Vaccins ()</term>
<term>Vaccins (immunologie)</term>
<term>Virus du SRAS (immunologie)</term>
<term>Épitopes ()</term>
<term>Épitopes (immunologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Epitopes</term>
<term>Peptides</term>
<term>Protein Subunits</term>
<term>Recombinant Fusion Proteins</term>
<term>Vaccines</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Peptides</term>
<term>Recombinant Fusion Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies</term>
<term>Epitopes</term>
<term>Peptides</term>
<term>Protein Subunits</term>
<term>Vaccines</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Nanoparticles</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Peptides</term>
<term>Protéines de fusion recombinantes</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps</term>
<term>Peptides</term>
<term>Sous-unités de protéines</term>
<term>Syndrome respiratoire aigu sévère</term>
<term>Vaccins</term>
<term>Virus du SRAS</term>
<term>Épitopes</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term>
<term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Recombinant Fusion Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines de fusion recombinantes</term>
</keywords>
<keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Nanoparticles</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Humans</term>
<term>Mice</term>
<term>Mice, Inbred BALB C</term>
<term>Models, Molecular</term>
<term>Molecular Sequence Data</term>
<term>Protein Conformation</term>
<term>Sequence Homology, Amino Acid</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Conformation des protéines</term>
<term>Données de séquences moléculaires</term>
<term>Humains</term>
<term>Modèles moléculaires</term>
<term>Nanoparticules</term>
<term>Peptides</term>
<term>Protéines de fusion recombinantes</term>
<term>Similitude de séquences d'acides aminés</term>
<term>Souris</term>
<term>Souris de lignée BALB C</term>
<term>Sous-unités de protéines</term>
<term>Syndrome respiratoire aigu sévère</term>
<term>Séquence d'acides aminés</term>
<term>Test ELISA</term>
<term>Vaccins</term>
<term>Épitopes</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) is an infectious disease caused by a novel coronavirus that cost nearly 800 lives. While there have been no recent outbreaks of the disease, the threat remains as SARS coronavirus (SARS-CoV) like strains still exist in animal reservoirs. Therefore, the development of a vaccine against SARS is in grave need. Here, we have designed and produced a prototypic SARS vaccine: a self-assembling polypeptide nanoparticle that repetitively displays a SARS B-cell epitope from the C-terminal heptad repeat of the virus' spike protein. Biophysical analyses with circular dichroism, transmission electron microscopy and dynamic light scattering confirmed the computational design showing alpha-helcial nanoparticles with sizes of about 25 nm. Immunization experiments with no adjuvants were performed with BALB/c mice. An investigation of the binding properties of the elicited antibodies showed that they were highly conformation specific for the coiled-coil epitope because they specifically recognized the native trimeric conformation of C-terminal heptad repeat region. Consequently, the antisera exhibited neutralization activity in an in vitro infection inhibition assay. We conclude that these peptide nanoparticles represent a promising platform for vaccine design, in particular for diseases that are characterized by neutralizing epitopes with coiled-coil conformation such as SARS-CoV or other enveloped viruses.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19152635</PMID>
<DateCompleted><Year>2009</Year>
<Month>02</Month>
<Day>19</Day>
</DateCompleted>
<DateRevised><Year>2018</Year>
<Month>11</Month>
<Day>13</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1747-0285</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>73</Volume>
<Issue>1</Issue>
<PubDate><Year>2009</Year>
<Month>Jan</Month>
</PubDate>
</JournalIssue>
<Title>Chemical biology & drug design</Title>
<ISOAbbreviation>Chem Biol Drug Des</ISOAbbreviation>
</Journal>
<ArticleTitle>Peptide nanoparticles as novel immunogens: design and analysis of a prototypic severe acute respiratory syndrome vaccine.</ArticleTitle>
<Pagination><MedlinePgn>53-61</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1111/j.1747-0285.2008.00746.x</ELocationID>
<Abstract><AbstractText>Severe acute respiratory syndrome (SARS) is an infectious disease caused by a novel coronavirus that cost nearly 800 lives. While there have been no recent outbreaks of the disease, the threat remains as SARS coronavirus (SARS-CoV) like strains still exist in animal reservoirs. Therefore, the development of a vaccine against SARS is in grave need. Here, we have designed and produced a prototypic SARS vaccine: a self-assembling polypeptide nanoparticle that repetitively displays a SARS B-cell epitope from the C-terminal heptad repeat of the virus' spike protein. Biophysical analyses with circular dichroism, transmission electron microscopy and dynamic light scattering confirmed the computational design showing alpha-helcial nanoparticles with sizes of about 25 nm. Immunization experiments with no adjuvants were performed with BALB/c mice. An investigation of the binding properties of the elicited antibodies showed that they were highly conformation specific for the coiled-coil epitope because they specifically recognized the native trimeric conformation of C-terminal heptad repeat region. Consequently, the antisera exhibited neutralization activity in an in vitro infection inhibition assay. We conclude that these peptide nanoparticles represent a promising platform for vaccine design, in particular for diseases that are characterized by neutralizing epitopes with coiled-coil conformation such as SARS-CoV or other enveloped viruses.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Pimentel</LastName>
<ForeName>Tais A P F</ForeName>
<Initials>TA</Initials>
<AffiliationInfo><Affiliation>Institute of Materials Science, University of Connecticut, Storrs, CT 06269-3136, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Yan</LastName>
<ForeName>Zhe</ForeName>
<Initials>Z</Initials>
</Author>
<Author ValidYN="Y"><LastName>Jeffers</LastName>
<ForeName>Scott A</ForeName>
<Initials>SA</Initials>
</Author>
<Author ValidYN="Y"><LastName>Holmes</LastName>
<ForeName>Kathryn V</ForeName>
<Initials>KV</Initials>
</Author>
<Author ValidYN="Y"><LastName>Hodges</LastName>
<ForeName>Robert S</ForeName>
<Initials>RS</Initials>
</Author>
<Author ValidYN="Y"><LastName>Burkhard</LastName>
<ForeName>Peter</ForeName>
<Initials>P</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<GrantList CompleteYN="Y"><Grant><GrantID>P01 AI059576</GrantID>
<Acronym>AI</Acronym>
<Agency>NIAID NIH HHS</Agency>
<Country>United States</Country>
</Grant>
<Grant><GrantID>P01 AI059576-050003</GrantID>
<Acronym>AI</Acronym>
<Agency>NIAID NIH HHS</Agency>
<Country>United States</Country>
</Grant>
<Grant><GrantID>AI059576</GrantID>
<Acronym>AI</Acronym>
<Agency>NIAID NIH HHS</Agency>
<Country>United States</Country>
</Grant>
</GrantList>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D052061">Research Support, N.I.H., Extramural</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>Chem Biol Drug Des</MedlineTA>
<NlmUniqueID>101262549</NlmUniqueID>
<ISSNLinking>1747-0277</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D000906">Antibodies</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D000939">Epitopes</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D010455">Peptides</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D021122">Protein Subunits</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D011993">Recombinant Fusion Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D014612">Vaccines</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000906" MajorTopicYN="N">Antibodies</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004797" MajorTopicYN="N">Enzyme-Linked Immunosorbent Assay</DescriptorName>
<QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000939" MajorTopicYN="N">Epitopes</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008807" MajorTopicYN="N">Mice, Inbred BALB C</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008958" MajorTopicYN="N">Models, Molecular</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D053758" MajorTopicYN="Y">Nanoparticles</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010455" MajorTopicYN="N">Peptides</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011487" MajorTopicYN="N">Protein Conformation</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D021122" MajorTopicYN="N">Protein Subunits</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011993" MajorTopicYN="N">Recombinant Fusion Proteins</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017386" MajorTopicYN="N">Sequence Homology, Amino Acid</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D045169" MajorTopicYN="N">Severe Acute Respiratory Syndrome</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
<QualifierName UI="Q000517" MajorTopicYN="Y">prevention & control</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014612" MajorTopicYN="N">Vaccines</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2009</Year>
<Month>1</Month>
<Day>21</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2009</Year>
<Month>1</Month>
<Day>21</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2009</Year>
<Month>2</Month>
<Day>20</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">19152635</ArticleId>
<ArticleId IdType="pii">JPP746</ArticleId>
<ArticleId IdType="doi">10.1111/j.1747-0285.2008.00746.x</ArticleId>
<ArticleId IdType="pmc">PMC2756483</ArticleId>
<ArticleId IdType="mid">NIHMS133156</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2004 Apr 27;101(17):6641-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15096611</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 2004 May 14;279(20):20836-49</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14996844</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Lancet. 2004 Jun 26;363(9427):2122-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15220033</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Immunol. 2004 Sep 1;173(5):3148-54</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15322175</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochem Biophys Res Commun. 2004 Nov 12;324(2):773-81</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15474494</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 1988 Aug 25;263(24):11768-75</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2457026</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 1992 Nov;66(11):6695-705</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1383568</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Anal Biochem. 1992 Jun;203(2):295-301</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1416025</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 1993 Nov 26;262(5138):1401-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8248779</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Vet Microbiol. 1993 Nov;37(3-4):285-97</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8116187</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 1994 Sep 1;371(6492):80-3</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8072533</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 2004 Nov 19;279(47):49414-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15345712</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Vaccine. 2005 Mar 18;23(17-18):2273-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15755610</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2005 Oct 28;310(5748):676-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16195424</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Vaccine. 2006 Apr 24;24(17):3624-31</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16497416</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Anal Biochem. 2006 Jul 15;354(2):238-46</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16730633</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Struct Biol. 2006 Aug;155(2):176-94</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16697221</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Methods. 2006 Sep;40(1):1-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16997708</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Vaccine. 2007 Jan 8;25(4):630-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17011679</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Vaccine. 2007 Jan 8;25(4):729-40</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17049691</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nanomedicine. 2006 Jun;2(2):95-102</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17292121</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Vaccine. 2007 Apr 12;25(15):2832-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17092615</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Vaccine. 2007 Sep 28;25(39-40):6981-91</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17709158</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biophys J. 2000 Mar;78(3):1606-19</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10692345</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Anal Biochem. 2000 Mar 15;279(2):151-63</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10706784</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Protein Sci. 2000 Dec;9(12):2294-301</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11206050</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2001 Feb 27;98(5):2217-21</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11226219</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2003 May 30;300(5624):1399-404</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12730501</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2003 Oct 10;302(5643):276-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12958366</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Respirology. 2003 Nov;8 Suppl:S6-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15018126</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Vaccine. 2008 Feb 6;26(6):797-808</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18191004</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Vaccine. 2008 Apr 16;26(17):2164-74</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18346823</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochemistry. 2008 Jul 1;47(26):6802-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18540634</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Struct Mol Biol. 2008 Jul;15(7):690-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18596815</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>États-Unis</li>
</country>
<region><li>Connecticut</li>
</region>
</list>
<tree><noCountry><name sortKey="Burkhard, Peter" sort="Burkhard, Peter" uniqKey="Burkhard P" first="Peter" last="Burkhard">Peter Burkhard</name>
<name sortKey="Hodges, Robert S" sort="Hodges, Robert S" uniqKey="Hodges R" first="Robert S" last="Hodges">Robert S. Hodges</name>
<name sortKey="Holmes, Kathryn V" sort="Holmes, Kathryn V" uniqKey="Holmes K" first="Kathryn V" last="Holmes">Kathryn V. Holmes</name>
<name sortKey="Jeffers, Scott A" sort="Jeffers, Scott A" uniqKey="Jeffers S" first="Scott A" last="Jeffers">Scott A. Jeffers</name>
<name sortKey="Yan, Zhe" sort="Yan, Zhe" uniqKey="Yan Z" first="Zhe" last="Yan">Zhe Yan</name>
</noCountry>
<country name="États-Unis"><region name="Connecticut"><name sortKey="Pimentel, Tais A P F" sort="Pimentel, Tais A P F" uniqKey="Pimentel T" first="Tais A P F" last="Pimentel">Tais A P F. Pimentel</name>
</region>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Ncbi/Merge

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001E28 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd -nk 001E28 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    SrasV1
   |flux=    Ncbi
   |étape=   Merge
   |type=    RBID
   |clé=     pubmed:19152635
   |texte=   Peptide nanoparticles as novel immunogens: design and analysis of a prototypic severe acute respiratory syndrome vaccine.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/RBID.i   -Sk "pubmed:19152635" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd   \
       | NlmPubMed2Wicri -a SrasV1

This area was generated with Dilib version V0.6.33.
Data generation: Tue Apr 28 14:49:16 2020. Site generation: Sat Mar 27 22:06:49 2021

	Serveur d'exploration SRAS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration SRAS

Peptide nanoparticles as novel immunogens: design and analysis of a prototypic severe acute respiratory syndrome vaccine.

Peptide nanoparticles as novel immunogens: design and analysis of a prototypic severe acute respiratory syndrome vaccine.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki